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bioanalytical chemistry

Mingtai Sun, Xin Yang, Yuannian Zhang, Suhua Wang, Ming Wah Wong, Runyan Ni, Dejian Huang
Herein we report on fluorophore-tagged Grubbs catalysts as turn-on fluorescent probes for the sensitive detection and quantitation of ethylene, a plant hormone that plays a critical role in many phases of plant growth and fruit ripening. The ruthenium (Ru) based weakly fluorescent probes were prepared handily through metathesis reaction between the first generation Grubbs catalyst and selected fluorophores that have high quantum yields and contain terminal vinyl groups. Upon exposure to ethylene, fluorescence enhancement was observed via the release of fluorophore from the probe...
December 3, 2018: Journal of Agricultural and Food Chemistry
Ke Yang, Huizhen Wang, Ning Ma, Ming Zeng, Huaiqing Luo, Dinggeng He
Synthetic DNA machines that operate on the nanoscale three-dimensional (3D) track have attracted rapidly increasing interest because of their potential in biocomputing, drug delivery, and biosensing applications. Current nanoscale 3D DNA tracks are typically created by self-assembling thiolated oligonucleotides at gold nanoparticles (AuNPs) surface via the strong Au-S chemistry. However, it remains challenging to precisely control the orientation and conformation of 3D DNA track on AuNPs surface and finely tune the hybridization ability of 3D track...
November 29, 2018: ACS Applied Materials & Interfaces
Mohammad Pousti, Maxime Joly, Patrice Roberge, Mehran Abbaszadeh Amirdehi, Andre Begin-Drolet, Jesse Greener
A fully automated linear scanning attenuated total reflection (ATR) accessory is presented for Fourier transform infrared (FTIR) spectrosco-py. The approach is based on the accurate displacement of a multi-bounce ATR crystal relative to a stationary infrared beam. To ensure accu-rate positioning and to provide a second sample characterization mode, a custom-built microscope was integrated into the system and the computerized work flow. Custom software includes automated control and measurement routines with a straightforward user interface for selecting parameters and monitoring experimental progress...
November 18, 2018: Analytical Chemistry
Evangelia G Chronopoulou, Nikolaos E Labrou
Glutathione transferases (GSTs) catalyze the conjugation of glutathione (GSH) to endogenous and xenobiotic electrophilic compounds and have been involved in the development of resistance toward cancer chemotherapeutic drugs and in the etiology, pathology and progression of several other diseases. In the present work, the human isoenzyme GSTA1-1 (hGSTA1-1) was used to assemble a microplate-based platform for high-throughput screening of inhibitors from plant extracts. The enzyme was immobilized using sol-gel chemistry and deposited as a layer at the bottom surface of 96-well format ELISA microplate...
October 28, 2018: Current Pharmaceutical Biotechnology
Selvakumar Palanisamy, Vijayalakshmi Velusamy, Shih-Wen Chen, Thomas C K Yang, Sridharan Balu, Craig E Banks
In recent years, the carbohydrate polymers incorporated composite materials have shown significant interest in the bioanalytical chemistry due to their enhanced catalytic performances of various enzymes or mimics. This paper reports the fabrication of novel H2 O2 biosensor using a hemin immobilized reduced graphene oxide-cellulose microfiber composite (hemin/RGO-CMF). The RGO-CMF composite was prepared by the reduction of GO-CMF composite using vitamin C as a reducing agent. Various physio-chemical methods have applied for the characterization of RGO-CMF composite...
January 15, 2019: Carbohydrate Polymers
Pradip Das, Abootaleb Sedighi, Ulrich J Krull
The development of bioanalytical methods that provide early detection of the presence of cancer by sensitive and specific determination of biomarkers such as small biomolecules, nucleic acids, proteins, enzymes, and even whole cells are essential to improve opportunity for improved patient treatment and to diminish the rate of cancer mortality. Förster resonance energy transfer (FRET) methods have been increasingly used to develop bioassays that offer speed, selectivity and low detection levels with practicality that is appropriate for providing point-of-care measurements for screening...
December 24, 2018: Analytica Chimica Acta
Guanyue Gao, Dengchao Wang, Ricardo Brocenschi, Jinfang Zhi, Michael V Mirkin
Fast and cost-efficient detection and identification of bacteria in food and water samples and biological fluids is an important challenge in bioanalytical chemistry. It was shown recently that bacteria can be detected by measuring the decrease in the diffusion current to the ultramicroelectrode caused by cell collisions with its surface. To add selectivity to the bacteria detection, herein we show the possibility of collision experiments with the signal produced by electrochemical activity of bacterial cells reducing (or oxidizing) redox species...
September 27, 2018: Analytical Chemistry
Lisa A Holland, Jeffrey S Carver, Lindsay M Veltri, Rachel J Henderson, Kimberly D Quedado
Nanotechnology is a broad field combining traditional scientific disciplines; however, analytical chemistry plays an important role in material design, synthesis, characterization, and application. This article emphasizes the uniqueness of nanotechnology and the importance of providing high-quality undergraduate research experiences to both attract and retain talented individuals to the field of nanotechnology. In response to this need to develop a strong and sustainable nanotechnology work force, strategies to create authentic research experiences are considered within the framework of an interdisciplinary nanotechnology environment at West Virginia University...
September 2018: Analytical and Bioanalytical Chemistry
Takashi Kaneta
Laser-induced fluorometry (LIF) has achieved the detection of single molecules, which ranks it among the most sensitive of detection techniques, whereas capillary electrophoresis (CE) is known as a powerful separation method with resolution that is beyond the reach of many other types of chromatography. Therefore, a coupling of LIF with CE has established an unrivaled analytical technique in terms of sensitivity and resolution. CE-LIF has demonstrated excellent performance in bioanalytical chemistry for the high-resolution separation and highly sensitive detection of DNAs, proteins, and small bioactive molecules...
August 6, 2018: Chemical Record: An Official Publication of the Chemical Society of Japan ... [et Al.]
Aafrin M Pettiwala, Prabhat K Singh
Constructing sensor systems for rapid and selective detection of small biomolecules such as amino acids is a major area of focus in bioanalytical chemistry. Considering the biological relevance of arginine and lysine, significant efforts have been directed to develop fluorescent sensors for their detection. However, these developed sensors suffer from certain disadvantages such as poor aqueous solubility, technically demanding and time-consuming synthetic protocols, and more importantly, most of them operate through single wavelength measurements, making their performance prone to small variations in experimental conditions...
December 31, 2017: ACS Omega
Lei Chen, Biwu Liu, Zhangrun Xu, Juewen Liu
Selective extraction of a small amount of nucleic acids from complex biological samples containing a high concentration of proteins is critical for bioanalytical chemistry. A number of previously published studies have focused on long, double-stranded DNA such as plasmid DNA. On the other hand, we are interested in short oligonucleotides. Nucleic acids have a negatively charged phosphate backbone that interacts with metal oxides strongly, and this may be used to distinguish them from proteins. In this work, a few metal oxide nanoparticles were screened, including NiO, CoO, ZnO, TiO2 , CeO2 , and Fe3 O4 for DNA recovery...
August 7, 2018: Langmuir: the ACS Journal of Surfaces and Colloids
S Mukherjee, J Á Martínez-González, D P Dowling, A A Gowen
The static water contact angle (CA) quantifies the degree of wetting that occurs when a surface encounters a liquid, e.g. water. This property is a result of factors such as surface chemistry and local roughness and is an important analytical parameter linked to the suitability of a surface for a given bioanalytical process. Monitoring the spatial variation in wettability over surfaces is increasingly critical to analysts and manufacturers for improved quality control. However, CA acquisition is often time-consuming because it involves measurements over multiple spatial locations, independent sampling and the need for a single instrument operator...
July 23, 2018: Analyst
Natalia Miękus, Ilona Olędzka, Darya Harshkova, Ivan Liakh, Alina Plenis, Piotr Kowalski, Tomasz Bączek
The determination of neurotransmitters (NTs) as relevant potential biomarkers in the study of various central nervous system (CNS) pathologies has been demonstrated. Knowing that NTs-related diseases mostly occupy individual regions of the nervous system, as observed, for instance, in neurodegenerative diseases (Alzheimer’s and Parkinson’s Diseases), the analysis of brain slices is preferred to whole-brain analysis. In this report, we present sample preparation approaches, such as solid-phase extraction, solid-phase microextraction, and dispersive liquid⁻liquid microextraction, and discuss the pitfalls and advantages of each extraction method...
May 24, 2018: International Journal of Molecular Sciences
Michael Z Kamrath, Thomas R Rizzo
Ion mobility spectrometry (IMS) has become a valuable tool in biophysical and bioanalytical chemistry because of its ability to separate and characterize the structure of gas-phase biomolecular ions on the basis of their collisional cross section (CCS). Its importance has grown with the realization that in many cases, biomolecular ions retain important structural characteristics when produced in the gas phase by electrospray ionization (ESI). While a CCS can help distinguish between structures of radically different types, one cannot expect a single number to differentiate similar conformations of a complex molecule...
June 19, 2018: Accounts of Chemical Research
Andreas Bietenbeck, Wolf J Geilenkeuser, Frank Klawonn, Michael Spannagl, Matthias Nauck, Astrid Petersmann, Markus A Thaler, Christof Winter, Peter B Luppa
BACKGROUND: Determination of blood glucose concentration is one of the most important measurements in clinical chemistry worldwide. Analyzers in central laboratories (CL) and point-of-care tests (POCT) are both frequently used. In Germany, regular participation in external quality assessment (EQA) schemes is mandatory for laboratories performing glucose testing. METHODS: Glucose testing data from the two German EQAs "Reference Institute for Bioanalytics" (RfB) and "INSTAND - Gesellschaft zur Förderung der Qualitätssicherung in medizinischen Laboratorien" (Instand) were analyzed from 2012 to 2016...
July 26, 2018: Clinical Chemistry and Laboratory Medicine: CCLM
Igor A Kaltashov, Jake W Pawlowski, Wenhua Yang, Khaja Muneeruddin, Honglin Yao, Cedric E Bobst, Andrei N Lipatnikov
Interfacing liquid chromatography (LC) with electrospray ionization (ESI) to enable on-line MS detection had been initially implemented using reversed phase LC, which in the past three decades remained the default type of chromatography used for LC/MS and LC/MS/MS studies of protein structure. In contrast, the advantages of other types of LC as front-ends for ESI MS, particularly those that allow biopolymer higher order structure to be preserved throughout the separation process, enjoyed relatively little appreciation until recently...
July 15, 2018: Methods: a Companion to Methods in Enzymology
Linlin Dong, Chao Li, Charles Locuson, Susan Chen, Mark G Qian
Plasma stability assessment under physiological temperature is an essential step for developing and optimizing antibody drug conjugate (ADC) molecules, especially those with cleavable linkers. The assessment of plasma stability often requires monitoring multiple analytes using a combination of bioanalytical assays for free payloads, conjugated payloads (or conjugated antibodies), total antibodies, and payloads that have migrated from antibodies to plasma constituent proteins. Bioanalytical assays are needed in early drug discovery to quickly screen diverse ADC candidates of different antibody constructs, linker variants, and antibody anchor sites...
May 15, 2018: Analytical Chemistry
Bo Zhang, Mouzhe Xie, Lei Bruschweiler-Li, Rafael Brüschweiler
Understanding and harnessing the interactions between nanoparticles and biological molecules is at the forefront of applications of nanotechnology to modern biology. Metabolomics has emerged as a prominent player in systems biology as a complement to genomics, transcriptomics and proteomics. Its focus is the systematic study of metabolite identities and concentration changes in living systems. Despite significant progress over the recent past, important challenges in metabolomics remain, such as the deconvolution of the spectra of complex mixtures with strong overlaps, the sensitive detection of metabolites at low abundance, unambiguous identification of known metabolites, structure determination of unknown metabolites and standardized sample preparation for quantitative comparisons...
March 13, 2018: Metabolites
Vincent Desfontaine, Francesca Capetti, Raul Nicoli, Tiia Kuuranne, Jean-Luc Veuthey, Davy Guillarme
Matrix effects (ME) is acknowledged as being one of the major drawbacks of quantitative bioanalytical methods, involving the use of liquid chromatography coupled to mass spectrometry (LC-MS). In the present study, the incidence of ME in SFC-MS/MS and LC-MS/MS in the positive mode electrospray ionization (ESI+) was systematically compared for the analysis of urine and plasma samples using two representative sets of 40 doping agents and 38 pharmaceutical compounds, respectively. Three different SFC stationary phase chemistries were employed, to highlight the importance of the column in terms of selectivity...
March 15, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Kevin D Clark, María J Trujillo-Rodríguez, Jared L Anderson
Ionic liquids are a class of solvents and materials that hold great promise in bioanalytical chemistry. Task-specific ionic liquids have recently been designed for the selective extraction, separation, and detection of proteins, peptides, nucleic acids, and other physiologically relevant analytes from complex biological samples. To facilitate rapid bioanalysis, ionic liquids have been integrated in miniaturized and automated procedures. Bioanalytical separations have also benefited from the modification of nonspecific magnetic materials with ionic liquids or the implementation of ionic liquids with inherent magnetic properties...
July 2018: Analytical and Bioanalytical Chemistry
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