Cailin E Deal, Angelene F Richards, Tracy Yeung, Max J Maron, Ziqiu Wang, Yen-Ting Lai, Brian R Fritz, Sunny Himansu, Elisabeth Narayanan, Ding Liu, Rositsa Koleva, Stuart Licht, Chiaowen J Hsiao, Ivana L Rajlic, Hillary Koch, Michael Kleyman, Mark E Pulse, William J Weiss, Jennifer E Doering, Samantha K Lindberg, Nicholas J Mantis, Andrea Carfi, Obadiah J Plante
Colonization of the gut and airways by pathogenic bacteria can lead to local tissue destruction and life-threatening systemic infections, especially in immunologically compromised individuals. Here, we describe an mRNA-based platform enabling delivery of pathogen-specific immunoglobulin A (IgA) monoclonal antibodies into mucosal secretions. The platform consists of synthetic mRNA encoding IgA heavy, light, and joining (J) chains, packaged in lipid nanoparticles (LNPs) that express glycosylated, dimeric IgA with functional activity in vitro and in vivo...
October 27, 2023: Cell reports medicine