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Salmonella AND cancer

Luis Humberto De Loera Rodríguez, Genaro Gabriel Ortiz, Paloma Rivero Moragrega, Irma Ernestina Velázquez Brizuela, Juan Francisco Santoscoy Gutiérrez, Ana Rosa Rincón Sánchez, Claudia Lisette Charles Niño, José Antonio Cruz Serrano, Alfredo De Jesús Celis de la Rosa, Fermín Paul Pacheco Moisés, María Del Refugio Medrano González
BACKGROUND: patients with cervical cancer (CC) receiving chemotherapy and radiotherapy have several gastrointestinal adverse effects. OBJECTIVE: to evaluate the effect of dietary symbiotic supplementation on fecal calprotectin (FCP), bacterial DNA levels, and gastrointestinal adverse effects in patients with CC. METHODS: clinical, controlled, randomized, double-blind trial. Patients consumed symbiotics or placebo three times a day for seven weeks...
December 3, 2018: Nutrición Hospitalaria: Organo Oficial de la Sociedad Española de Nutrición Parenteral y Enteral
Muhammad Shahid Riaz Rajoka, Hafiza Mahreen Mehwish, Hafiz Fakhar Hayat, Nazim Hussain, Sobia Sarwar, Humaira Aslam, Ahmad Nadeem, Junling Shi
The natural antioxidant agent is urgently needed to prevent the negative effects of newly generated free radicals and chronic disorders. Recently, the microbial exopolysaccharide (EPS) is currently used as a potential biopolymer due to its unique biological characteristics. In this study, the biological potential was carried out on the EPSs produced by Lactobacillus reuteri SHA101 (EPS-lr) and Lactobacillus vaginalis SHA110 (EPS-lvg) isolated from gut cecum samples of healthy poultry birds (hen). As results, the EPS-lr and EPS-lvg showed the emulsifying activity of 37...
December 3, 2018: Probiotics and Antimicrobial Proteins
K Karthik, S Dhanuskodi, C Gobinath, S Prabukumar, S Sivaramakrishnan
Magnesium oxide (MgO) nanostructures were prepared using microwave-assisted (M 1) and hydrothermal (M 2) methods and characterized by XRD, SEM and FT-IR. It exhibits cubic structure with an average crystallite size of 20 nm (M 1) and 14 nm (M 2) and the lattice strain (WH plot) is 0.0017 (M 1), 0.0037 (M 2). It's spherical and rods like structures are confirmed through SEM and TEM. The vibrational stretching mode of MgO is 439 (M 1) and 449 cm-1 (M 2). The optical bandgap is estimated as 5.93 eV (M 1) and 5...
November 12, 2018: Journal of Photochemistry and Photobiology. B, Biology
Tingtao Chen, Xiaoxiao Zhao, Yimeng Ren, Yuqing Wang, Xianyao Tang, Puyuan Tian, Huan Wang, Hongbo Xin
The strong human immunity and the associated toxicities of attenuated Salmonella severely limit the clinical use of Salmonella in tumour suppression. In the present study, we constructed an engineered VNP20009-DNase I strain and evaluated the synergistic effects of triptolide (TPL) and VNP20009-DNase I against melanoma in mice. Our results indicated that TPL could significantly inhibit the cell growth and cell migration and significantly enhanced the apoptosis rate of B16F10 cells in vitro. The in vivo results indicated that TPL markedly improved tumour colonisation of VNP20009-DNase I and led to a larger necrotic area in the melanoma...
November 17, 2018: Applied Microbiology and Biotechnology
Crislaine Maria Carvalho da Cruz Brambilla, Ana Leticia Hilario Garcia, Fernanda Rabaioli da Silva, Silvio Roberto Taffarel, Ivana Grivicich, Jaqueline Nascimento Picada, Amanda Scotti, Daiana Dalberto, Miroslav Mišík, Siegfried Knasmüller, Juliana da Silva
Acid Black 10B (AB10B) is widely used for the production of textiles, leather and prints. It is a representative of azo dyes and it is well documented that some of these compounds are mutagenic per se, and that cleavage products (in particular aromatic amines) may cause damage of the genetic material and cancer. Since no toxicological data on AB10B have been published, we evaluated its mutagenic activity in Salmonella/microsome assays and studied its acute toxic and genotoxic properties in a human derived liver cell line (HepG2) which retained the activities of drug metabolizing enzymes...
November 3, 2018: Chemosphere
Jasneet Kaur, Manjot Singh, Carmela Dell'Aversana, Rosaria Benedetti, Paola Giardina, Manuela Rossi, Mohammadhassan Valadan, Alessandro Vergara, Anna Cutarelli, Angela Michela Immacolata Montone, Lucia Altucci, Federica Corrado, Angela Nebbioso, Carlo Altucci
Two dimensional materials beyond graphene such as MoS2 and WS2 are novel and interesting class of materials whose unique physico-chemical properties can be exploited in applications ranging from leading edge nanoelectronics to the frontiers between biomedicine and biotechnology. To unravel the potential of TMD crystals in biomedicine, control over their production through green and scalable routes in biocompatible solvents is critically important. Furthermore, considering multiple applications of eco-friendly 2D dispersions and their potential impact onto live matter, their toxicity and antimicrobial activity still remain an open issue...
November 6, 2018: Scientific Reports
David Quintero, Jamie Carrafa, Lena Vincent, Hee Jong Kim, James Wohlschlegel, David Bermudes
Sunflower trypsin inhibitor (SFTI) is a 14 amino acid bicyclic peptide that contains a single internal disulfide bond. We initially constructed chimeras of SFTI with N-terminal secretion signals from the Escherichia coli and the Pseudomonas aeruginosa , but only detected small amounts of protease inhibition resulting from these constructs. A substantially higher degree of protease inhibition was detected from a C-terminal SFTI fusion with E. coli YebF, which radiated more than a centimeter from a single colony of E...
October 19, 2018: Journal of Microbiology and Biotechnology
Kentaro Miyake, Tasuku Kiyuna, Masuyo Miyake, Ming Zhao, Sintawat Wangsiricharoen, Kei Kawaguchi, Zhiying Zhang, Takashi Higuchi, Sahar Razmjooei, Yunfeng Li, Scott D Nelson, Tara Russell, Arun Singh, Takashi Murakami, Yukihiko Hiroshima, Masashi Momiyama, Ryusei Matsuyama, Takashi Chishima, Shree Ram Singh, Sant P Chawla, Fritz C Eilber, Itaru Endo, Robert M Hoffman
Cancer of unknown primary (CUP) is metastatic disease without a known primary and therefore very difficult to identify effective therapy. Previously, we demonstrated partial efficacy of Salmonella typhimurium A1-R (S. typhimurium A1-R) alone and carboplatinum alone (CAR) on a CUP patient tumor in the patient-derived xenograft (PDOX) model. The aim of the present study was to investigate the efficacy of S. typhimurium A1-R combined with CAR on the CUP PDOX model. The CUP tumors were implanted orthotopically into the left supraclavicular fossa of nude mice to match the site from which they were resected from the patient...
October 2018: Tissue & Cell
Marco Antonio Hernández-Luna, Rosendo Luria-Pérez
In recent years, cancer immunotherapy has undergone great advances because of our understanding of the immune response and the mechanisms through which tumor cells evade it. A century after the first immunotherapy attempt based on bacterial products described by William Coley, the use of live attenuated bacterial vectors has become a promising alternative in the fight against cancer. This review describes the role of live attenuated Salmonella enterica as an oncolytic and immunotherapeutic agent, due to its high affinity for tumor tissue and its ability to activate innate and adaptive antitumor immune response...
2018: Journal of Immunology Research
Ines Rodrigues Lopes, Ricardo Jorge Silva, Ines Caramelo, Ana Eulalio, Miguel Mano
MicroRNAs (miRNAs) are small non-coding RNAs that post-transcriptionally modulate gene expression and orchestrate a wide range of biological and pathological processes. The use of high-throughput screening technologies, in particular microscopy-based screenings (also known as high-content screenings), coupled with genome-wide libraries for modulation of miRNA levels, allow for comprehensive functional analysis of each member of the miRNome in different phenotypic cell-based assays. The wealth of information obtained from such screenings spans across various fields of research, including cancer, cardiovascular, cell reprogramming, and infection biology...
October 5, 2018: Methods: a Companion to Methods in Enzymology
Tasuku Kiyuna, Yasunori Tome, Takashi Murakami, Kentaro Miyake, Kentaro Igarashi, Kei Kawaguchi, Hiromichi Oshiro, Takashi Higuchi, Masuyo Miyake, Norihiko Sugisawa, Zhiying Zhang, Sahar Razmjooei, Sintawat Wangsiricharoen, Bartosz Chmielowski, Scott D Nelson, Tara A Russell, Sarah M Dry, Yunfeng Li, Mark A Eckardt, Arun S Singh, Sant Chawla, Fuminori Kanaya, Fritz C Eilber, Shree Ram Singh, Ming Zhao, Robert M Hoffman
Myxofibrosarcoma (MFS) is the most common sarcomas in elderly patients and is either chemo-resistant or recurs with metastasis after chemotherapy. This recalcitrant cancer in need of improved treatment. We have established a patient-derived orthotopic xenograft (PDOX) of MFS. The MFS PDOX model was established in the biceps femoris of nude mice and randomized into 7 groups of 7 mice each: control; doxorubicin (DOX); pazopanib (PAZ); temozolomide (TEM); Irinotecan (IRN); IRN combined with TEM; IRN combined with cisplatinum (CDDP) and Salmonella typhimurium A1-R (S...
November 2, 2018: Biochemical and Biophysical Research Communications
Sandhanasamy Devanesan, Mohamad S AlSalhi, Radhakrishnan Vishnu Balaji, Amirtham Jacob A Ranjitsingh, Anis Ahamed, Akram A Alfuraydi, Fulwah Y AlQahtani, Fadilah S Aleanizy, Ahmed H Othman
To address the growing challenges from drug-resistant microbes and tumor incidence, approaches are being undertaken to phytosynthesize metal nanoparticles, particularly silver nanoparticles, to get remedial measure. In this study, an attempt has been made to utilize a major biowaste product, pomegranate fruit peel (Punica granatum), to synthesize silver nanoparticles. The silver nanoparticles (AgNPs) were synthesized using the aqueous extract of pomegranate peel. The formation of synthesized AgNPs was confirmed through UV-Vis spectroscopy, X-ray diffraction (XRD), transmission electron microscopy (TEM), scanning electron microscopy (SEM), and energy-dispersive X-ray spectroscopy (EDX) as well as through the change of the colorless aqueous solution to a dark brown solution...
October 4, 2018: Nanoscale Research Letters
Jelena Đorđević, Stoimir Kolarević, Jovana Jovanović, Jovana Kostić-Vuković, Irena Novaković, Marko Jeremić, Dušan Sladić, Branka Vuković-Gačić
Tert-butylquinone (TBQ) and its alkylamino and aralkylamino derivatives are of high interest as a potential antitumor agent. Therefore, it was necessary to investigate if the compounds exert undesirable activities such as interaction with DNA molecule which could result in negative side effects in the case of their use in the diseases treatment. The major aim of this study was to investigate genotoxic potential of TBQ and selected derivatives in an acellular model by using plasmid DNA, in the prokaryotic model by the SOS/umuC assay in Salmonella typhimurium TA1535/pSK1002 and in eukaryotic models by using comet assay in human fetal lung cell line (MRC-5) and human liver cancer cell line (HepG2)...
September 27, 2018: Drug and Chemical Toxicology
Abraham Eisenstark
Prostate cancer remains a life-threatening disease of men. While early detection has been helpful to reduce the mortality rate, we currently do not have a desired therapy. In recent years, new strategies have been proposed to treat prostate cancers with poor prognosis by utilizing genetically modified bacteria, including Salmonella typhimurium that preferentially replicate within solid tumors (1000:1 and up to 10,000:1 compared to non-cancerous tissue) destroying cancer cells without causing septic shock that is typically associated with wild-type S...
2018: Advances in Experimental Medicine and Biology
Kalyani Ektate, Maria Cristina Munteanu, Harshini Ashar, Jerry Malayer, Ashish Ranjan
Using attenuated Salmonella that efficiently homes in solid tumors, here we developed thermobots that actively transported membrane attached low-temperature sensitive liposome (LTSL) inside colon cancer cells for triggered doxorubicin release and simultaneous polarized macrophages to M1 phenotype with high intensity focused ultrasound (HIFU) heating (40-42 °C). Biocompatibility studies showed that the synthesized thermobots were highly efficient in LTSL loading without impacting its viability. Thermobots demonstrated efficient intracellular trafficking, high nuclear localization of doxorubicin, and induced pro-inflammatory cytokine expression in colon cancer cells in vitro...
August 30, 2018: Scientific Reports
Kei Kawaguchi, Takashi Higuchi, Shukuan Li, Qinghong Han, Yuying Tan, Kentaro Igarashi, Ming Zhao, Kentaro Miyake, Tasuku Kiyuna, Masuyo Miyake, Hiromichi Ohshiro, Norihiko Sugisawa, Zhiying Zhang, Sahar Razmjooei, Sintawat Wangsiricharoen, Bartosz Chmielowski, Scott D Nelson, Tara A Russell, Sarah M Dry, Yunfeng Li, Mark A Eckardt, Arun S Singh, Shree Ram Singh, Fritz C Eilber, Michiaki Unno, Robert M Hoffman
Melanoma is a recalcitrant cancer. To improve and individualize treatment for this disease, we previously developed a patient-derived orthotopic xenograft (PDOX) model for melanoma. We previously reported the individual efficacy of tumor-targeting Salmonella typhimurium A1-R (S. typhimurium A1-R) and recombinant methioninase (rMETase) for melanoma in the PDOX models of this disease. In the present study, we evaluated the efficacy of the combination of S. typhimurium A1-R with orally-administered rMETase (o-rMETase) for BRAF-V600E-negative melanoma in a PDOX model...
September 18, 2018: Biochemical and Biophysical Research Communications
Saloni Kakkar, Sumit Tahlan, Siong Meng Lim, Kalavathy Ramasamy, Vasudevan Mani, Syed Adnan Ali Shah, Balasubramanian Narasimhan
BACKGROUND: A new series of benzoxazole analogues was synthesized and checked for their in vitro antibacterial, antifungal and anticancer activities. RESULTS AND DISCUSSION: The synthesized benzoxazole compounds were confirmed by IR, 1 H/13 C-NMR, mass and screened for their in vitro antimicrobial activity against Gram-positive bacterium: Bacillus subtilis, four Gram-negative bacteria: Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Salmonella typhi and two fungal strains: Candida albicans and Aspergillus niger using tube dilution technique and minimum inhibitory concentration (MIC) was noted in µM and compared to ofloxacin and fluconazole...
August 12, 2018: Chemistry Central Journal
Síle A Johnson, Michael J Ormsby, Anne McIntosh, Stephen W G Tait, Karen Blyth, Daniel M Wall
Bacterial-mediated cancer therapy has shown great promise in in vivo tumour models with increased survival rates post-bacterial treatment. Improving efficiency of bacterial-mediated tumour regression has focused on controlling and exacerbating bacterial cytotoxicity towards tumours. One mechanism that has been used to carry this out is the process of bactofection where post-invasion, bacteria deliver plasmid-borne mammalian genes into target cells for expression. Here we utilised the cancer-targeting Salmonella Typhimurium strain, SL7207, to carry out bactofection into triple negative breast cancer MDA-MB-231 cells...
August 13, 2018: Cancer Gene Therapy
Leon M T Dicks, Lasse S Mikkelsen, Erik Brandsborg, Harold Marcotte
Clostridium difficile is normally present in low numbers in a healthy adult gastro-intestinal tract (GIT). Drastic changes in the microbial population, e.g., dysbiosis caused by extensive treatment with antibiotics, stimulates the growth of resistant strains and the onset of C. difficile infection (CDI). Symptoms of infection varies from mild diarrhea to colitis (associated with dehydration and bleeding), pseudomembranous colitis with yellow ulcerations in the mucosa of the colon, to fulminant colitis (perforation of the gut membrane), and multiple organ failure...
August 6, 2018: Current Microbiology
Christiane Fueldner, Janine Kohlschmidt, Sina Riemschneider, Felix Schulze, Katharina Zoldan, Charlotte Esser, Sunna Hauschildt, Jörg Lehmann
Polycyclic aromatic hydrocarbons such as benzo(a)pyrene (BaP) are environmental contaminants known to be immunosuppressive. Most effects of BaP towards immune cells are thought to be mediated through activation of the aryl hydrocarbon receptor (AhR). The AhR is a ligand-activated transcription factor, which plays a critical modulatory role in various cells during immune response. Macrophages are key players in innate immunity against intracellular bacteria and are discussed to be a target of AhR-mediated immune regulation...
November 1, 2018: Toxicology
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