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cytometri of flow

Inês Pereira, Flávia Sousa, Patrick Kennedy, Bruno Sarmento
Bioengineered functionalized nanoparticles have extensively been proposed in recent years to efficiently deliver anti-cancer drugs to the tumour site, by targeting the cancer cells and improving the therapeutic efficiency of active molecules. In this work, polymeric poly (lactic-co- glycolic)-polyethyleneglycol (PLGA-PEG) nanoparticles were produced by nanoprecipitation and loaded with paclitaxel, following surface-functionalized with a monoclonal antibody targeting the carcinoembryonic antigen (CEA) of intestinal epithelial cells...
August 12, 2018: International Journal of Pharmaceutics
Thao V Nguyen, Andrea C Alfaro, Fabrice Merien, Tim Young, Roffi Grandiosa
Massive mortalities due to pathogens are routinely reported in bivalve cultivation that have significant economic consequences for the global aquaculture industry. However, host-pathogen interactions and infection mechanisms that mediate these interactions are poorly understood. In addition, gender-specific immunological responses have been reported for some species, but the reasons for such differences have not been elucidated. In this study, we used a GC/MS-based metabolomics platform and flow cytometry approach to characterize metabolic and immunological responses in haemolymph of male and female mussels (Perna canaliculus) experimentally infected with Vibrio sp...
August 12, 2018: Journal of Invertebrate Pathology
Bjarne Larsen, Kyle Gardner, Carsten Pedersen, Marian Ørgaard, Zoë Migicovsky, Sean Myles, Torben Bo Toldam-Andersen
In recent years, new genome-wide marker systems have provided highly informative alternatives to low density marker systems for evaluating plant populations. To date, most apple germplasm collections have been genotyped using low-density markers such as simple sequence repeats (SSRs), whereas only a few have been explored using high-density genome-wide marker information. We explored the genetic diversity of the Pometum gene bank collection (University of Copenhagen, Denmark) of 349 apple accessions using over 15,000 genome-wide single nucleotide polymorphisms (SNPs) and 15 SSR markers, in order to compare the strength of the two approaches for describing population structure...
2018: PloS One
Miroslav Koblížek, Anastasia Lebedeva, Karel Fišer
The Flow Cytometry Standard (FCS) format is a widely accepted norm for storing Flow Cytometry (FCM) data. Its goal as a standard is to allow FCM data sharing and re-analysis. Over more than three decades of its existence FCS has evolved into a well-defined, flexible file format reflecting technical changes in the FCM field. Its flexibility as well as rising numbers of instrument vendors leads to suboptimal implementations of FCS in some cases. Such situations compromise the primary goal of the standard and hinder the ability to reproduce FCM analyses...
August 15, 2018: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
Giovanna Radicchio, Valeria Colicchia, Ramona Marrapodi, Maurizio Carbonari
The use of formamide for the study in flow cytometry of cell cycle phases, by DNA content measurement in human cancer cell lines, was recently published. In this manuscript, we verify the possibility of extending the procedure to simultaneous analysis of other parameters. The results obtained, here reported, show that the treatment of samples by formamide is compatible with the simultaneous detection of DNA content and surface phenotypes, with quantification of replicating DNA and with measurement of cells with fractional content of DNA...
August 15, 2018: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
Irina V Khalo, Viktoriya S Kozyreva, Roman V Vakhrushev, Daria S Patlai, Anna N Shilova, Andrei A Karpenko, Maxim A Yurkin, Alexander E Moskalensky, Dmitry I Strokotov, Valeri P Maltsev, Andrei V Chernyshev
We propose a calibration-free method to determine the number of receptors per cell, as well as the direct and the reverse reaction rate constants for a single receptor. The method is based on the analysis of the temporal evolution of the cells mean fluorescent intensity measured by a flow cytometer during the ligand-receptor (antigen-antibody) binding under the conditions of their comparable concentrations. We developed the kinetic approach accounting both for the delay between the dilution and the measurement and for the practical duration of the measurement itself...
August 15, 2018: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
Zheng Wang, Jingsong Ding, Shengjuan Luo, Peiqi Wang, Qi Liang
To prepare AS1411 targeted nano-ultrasonic contrast agent with liquid core, and to evaluate its ability for ultrasonic contrast enhancement and targeting MCF-7 cell in vitro.
 Methods: The modified solvent evaporation, self-synthesized membrane material and perfluorobrominane (PFOB) was used to form nano-ultrasonic contrast agent with PFOB core (nanoparticles, NP); then N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride and N-hydroxysuccinimide (EDC/NHS) catalysis was used to connect AS1411 to the surface of NP to prepare NP-AS1411...
June 28, 2018: Zhong Nan da Xue Xue Bao. Yi Xue Ban, Journal of Central South University. Medical Sciences
Yi-Ju Pan, Wei-Hsun Wang, Tzu-Yao Huang, Wei-Hsiang Weng, Chun-Kai Fang, Yu-Chan Chen, Jeng-Jong Hwang
OBJECTIVE AND DESIGN: To investigate the amelioration effects of quetiapine on rheumatoid arthritis with RAW 264.7 macrophage and collagen-induced arthritis (CIA) DBA/1J mouse model. SUBJECTS: RAW 264.7 macrophage and DBA/1J mice. TREATMENT: Lipopolysaccharide and collagen. METHODS: RAW 264.7 macrophages stimulated by lipopolysaccharide (LPS) followed by quetiapine treatments were investigated. Activations of CD80 and CD86 were analyzed by flow cytometry...
August 14, 2018: Inflammation Research: Official Journal of the European Histamine Research Society ... [et Al.]
Pallabita Chowdhury, Prashanth K B Nagesh, Sheema Khan, Bilal B Hafeez, Subhash C Chauhan, Meena Jaggi, Murali M Yallapu
The goal of this investigation was to develop and demonstrate a polymer/paclitaxel self-assembly (PTX-SA) formulation. Polymer/PTX-SAs were screened based on smaller size of formulation using dynamic light scattering analysis. Additionally, fluorescence microscopy and flow cytometry studies exhibited that polyvinylpyrrolidone (PVP)-based PTX-SAs (PVP/PTX-SAs) had superior cellular internalization capability in MCF7 and MDA-MB-231 breast cancer cells. The optimized PVP/PTX-SAs exhibited less toxicity to human red blood cells indicating a suitable formulation for reducing systemic toxicity...
July 2018: Acta Pharmaceutica Sinica. B
Itamar Luís Gonçalves, Liliana Rockenbach, Gustavo Machado das Neves, Gabriela Göethel, Fabiana Nascimento, Luciano Porto Kagami, Fabrício Figueiró, Gabriel Oliveira de Azambuja, Amanda de Fraga Dias, Andressa Amaro, Lauro Mera de Souza, Ivan da Rocha Pitta, Daiana Silva Avila, Daniel Fábio Kawano, Solange Cristina Garcia, Ana Maria Oliveira Battastini, Vera Lucia Eifler-Lima
An original and focused library of two sets of dihydropyrimidin-2-thiones (DHPMs) substituted with N-1 aryl groups derived from monastrol was designed and synthesized in order to discover a more effective Eg5 ligand than the template. Based on molecular docking studies, four ligands were selected to perform pharmacological investigations against two glioma cell lines. The results led to the discovery of two original compounds, called 20h and 20e , with an anti-proliferative effects, achieving IC50 values of about half that of the IC50 of monastrol in both cell lines...
June 1, 2018: MedChemComm
Jelena M Mašković, Antonios Hatzidimitriou, Ana Damjanović, Tatjana P Stanojković, Srećko R Trifunović, Athina A Geronikaki, Dionysia Papagiannopoulou
A new thiazole-containing multidentate ligand 2-((2-phenylthiazol-4-yl)methylthio)ethanamine, L, was synthesized and used to prepare new complexes of the formula PdII LCl2 (Pd-L), CuII L2 Cl2 (Cu-L) and fac -[Re/99m TcI (CO)3 (L)]+ (Re/99m Tc-L). The ligand L and the metal complexes were characterized spectroscopically. Furthermore, the structures of Re-L and Cu-L were elucidated by X-ray crystallography. Ligand L acts as a bidentate (Nth , S) chelator in Pd-L, as a bidentate (N, S) chelator in Cu-L and as a tridentate (Nth , S, N) chelator in Re-L...
May 1, 2018: MedChemComm
Zhe Wang, Xiangping Deng, Runde Xiong, Shujuan Xiong, Juan Liu, Xuan Cao, Xiaoyong Lei, Yanming Chen, Xing Zheng, Guotao Tang
A series of 3',4',5'-trimethoxy flavonoids with benzimidazole linked by different chain alkanes have been designed and synthesized. The potential activity of these compounds as anti-tumor agents was evaluated by cytotoxicity assay in MGC-803 (human gastric cancer), MCF-7 (human breast cancer), HepG-2 (human hepatoma) and MFC (mouse gastric cancer) tumor cell lines. Among them, compound 15 7-(3-(2-chloro-1 H -benzo[ d ]imidazol-1-yl)propoxy)-2-(3,4,5-trimethoxyphenyl)-4 H -chromen-4-one displayed the most potent antiproliferative activity, with IC50 values of 20...
February 1, 2018: MedChemComm
Faezeh Taghavi, Mostafa Gholizadeh, Amir Sh Saljooghi, Mohammad Ramezani
Cu(ii) immobilized on deferasirox loaded amine functionalized magnetic nanoparticles (Cu(ii)/Fe3 O4 @APTMS-DFX) as a novel magnetically recyclable heterogeneous catalyst is able to catalyze the [3 + 2] cycloaddition reactions of various organic nitriles with sodium azide. Using this method, a series of 5-substituted-1 H -tetrazoles under mild conditions in DMSO were prepared. The reaction involves mild reaction conditions with efficient transformation capability. The developed catalyst could be easily separated by applying an external magnetic field...
October 1, 2017: MedChemComm
Xu Wang, Long Jiang, Li-Yuan Sun, Yue Wu, Wen-Hui Wen, Xi-Fu Wang, Wei Liu, Yu-Jie Zhou, Lu-Ya Wang
Background: Familial hypercholesterolemia (FH) is an autosomal dominant disorder of lipoprotein metabolism which can lead to premature coronary heart disease (pCHD). There are about 3.8 million potential FH patients in China, whereas the clinical and genetic data of FH are limited. Methods: Dutch Lipid Clinic Network (DLCN) criteria was used to diagnose FH in outpatients with hypercholesterolemia. Resequencing chip analysis combined with Sanger sequencing validation were used to identify mutations in the definite FH patients according to DLCN criteria...
June 2018: Journal of Geriatric Cardiology: JGC
Roberto Moreno, Patricia Castro, Jan Vrána, Marie Kubaláková, Petr Cápal, Verónica García, Juan Gil, Teresa Millán, Jaroslav Doležel
A genetic linkage map of dioecious garden asparagus ( Asparagus officinalis L., 2 n = 2 x = 20) was constructed using F1 population, simple sequence repeat (SSR) and single nucleotide polymorphism (SNP) markers. In total, 1376 SNPs and 27 SSRs were used for genetic mapping. Two resulting parental maps contained 907 and 678 markers spanning 1947 and 1814 cM, for female and male parent, respectively, over ten linkage groups representing ten haploid chromosomes of the species. With the aim to anchor the ten genetic linkage groups to individual chromosomes and develop a tool to facilitate genome analysis and gene cloning, we have optimized a protocol for flow cytometric chromosome analysis and sorting in asparagus...
2018: Frontiers in Plant Science
Azadeh Mohammadi-Farsani, Mehryar Habibi-Roudkenar, Majid Golkar, Mohammad Ali Shokrgozar, Ali Jahanian-Najafabadi, Hossein KhanAhmad, Samira Valiyari, Saeid Bouzari
The NGR peptide is one of the well-known peptides for targeting tumor cells. It has the ability to target aminopeptidase N (CD13) on tumor cells or the tumor vascular endothelium. In this study, the NGR peptide was used for targeting A subunit of the Shiga toxin to cancer cells. The cytotoxic effect of the A-NGR fusion protein was assessed on HT1080, U937, HT29 cancer cells and MRC-5 normal cells. For this purpose, cells were treated with different concentrations of A-NGR (0.5-40 µg/ml). The evaluation of cell viability was achieved by MTT assay...
2018: EXCLI Journal
Jing Chen, Hai Liang Li, Bo Bo Li, Wei Li, Dong Ma, Yong He Li, Tao Liu
INTRODUCTION: Serum- and glucocorticoid-inducible kinase 3, a serine/threonine kinase that functions downstream of the PI3K signaling pathway, plays a critical role in neoplastic processes. It is expressed by various tumors and contributes to carcinogenesis. OBJECTIVE: The objective was to investigate serum- and glucocorticoid-inducible kinase 3 expression in nasopharyngeal carcinoma, to study the anti-tumor effects of serum- and glucocorticoid-inducible kinase 3 shRNA by inhibiting its expression in nasopharyngeal carcinoma cells and to discuss the potential implications of our findings...
July 18, 2018: Brazilian Journal of Otorhinolaryngology
Yaser Moghaddam, Alireza Andalib, Maryam Mohammad-Ganji, Vida Homayouni, Mohammadreza Sharifi, Mazdak Ganjalikhani-Hakemi
BACKGROUND: Acute Myeloid Leukemia (AML) is a Cancer of hematopoietic stem cells with a rapid progression. TIM-3 is expressed on leukemic stem cells (LSCs) in most types of AML and might have a positive effect on maintenance of malignant phenotype. MicroRNAs play important roles in either cancer progression or suppression. In this study were evaluated, the inhibitory effect of miR-498 on TIM-3 expression and its impact on proliferation and survival of HL-60 cell line. METHODS: Firstly, the probable inhibitory effect of miR-498 on TIM-3 expression was predicted...
July 24, 2018: Pathology, Research and Practice
Shengnuo Fan, Yuqiu Zheng, Xuan Liu, Wenli Fang, Xiaoyu Chen, Wang Liao, Xiuna Jing, Ming Lei, Enxiang Tao, Qiulan Ma, Xingmei Zhang, Rui Guo, Jun Liu
Alzheimer's disease is a neurodegenerative disorder mainly characterized by β-amyloid deposit and tau hyperphosphorylation with no curative treatments. Curcumin (Cur) has been proved to have potential use in Alzheimer's disease with its anti-amyloid, anti-inflammatory, and anti-oxidant properties, etc. However, its hydrophobicity and low bioavailability hinder its application. In this paper, we designed a novel brain-target nanoparticle, poly(lactide-co-glycolide)-block-poly(ethylene glycol) (PLGA-PEG) conjugated with B6 peptide and was loaded with Cur (PLGA-PEG-B6/Cur) and administered it into HT22 cells and APP/PS1 Al transgenic mice...
November 2018: Drug Delivery
Luping Zheng, Xiaolong Zhang, Yunfei Wang, Fangjun Liu, Jinlei Peng, Xuezhi Zhao, Huiru Yang, Liwei Ma, Baoyang Wang, Cong Chang, Hua Wei
The preparation of tumor acidic pH-cleavable polymers generally requires tedious post-polymerization modifications, leading to batch-to-batch variation and scale-up complexity. To develop a facile and universal strategy, we reported in this study design and successful synthesis of a dual functional monomer, a-OEGMA that bridges a methacrylate structure and oligo(ethylene glycol) (OEG) units via an acidic pH-cleavable acetal link. Therefore a-OEGMA integrates (i) the merits of commercially available oligo(ethylene glycol) monomethyl ether methacrylate (OEGMA) monomer, i...
August 14, 2018: Biomacromolecules
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