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Fibroblast Like Synoviocyte

Zhe Yun, Hua-Zhi Peng, Wei Wang, Qiong Ma, Jie Chen, Xin-Li Wang, Bao-An Ma
A growing number of studies suggest that synovitis plays an important role in the pathogenesis and progression of osteoarthritis (OA). As a negative mediator of the nuclear factor-kappa B (NF-κB) signaling pathway, the zinc finger protein A20 has significant anti-inflammatory properties. In this study, the differential expression of A20 was investigated at the mRNA and protein levels in human normal OA fibroblast-like synoviocytes (FLSs) and normal FLSs pretreated with TNF-α. We then measured the activation of the NF-κB pathway and expression of pro-inflammatory cytokines in the above three groups by western blotting, a human cytokine array and ELISA...
December 8, 2018: Biochemical and Biophysical Research Communications
Hu-Nan Sun, Ying-Hua Luo, Ling-Qi Meng, Xian-Ji Piao, Yue Wang, Jia-Ru Wang, Hao Wang, Yi Zhang, Jin-Qian Li, Wan-Ting Xu, Yang Liu, Yu Zhang, Tong Zhang, Ying-Hao Han, Mei-Hua Jin, Gui-Nan Shen, Yan-Qing Zang, Long-Kui Cao, Dong-Jie Zhang, Cheng-Hao Jin
The present study investigated the mechanisms of apoptosis induced by cryptotanshinone (CT) in human rheumatoid arthritis fibroblast‑like synoviocytes (RA‑FLSs). Cell Counting kit‑8 assay was performed to determine the cytotoxic effects of CT in human RA‑FLSs, including primary RA‑FLS, HFLS‑RA and MH7A cells, and in HFLS cells derived from normal synovial tissue. Annexin V‑FITC/PI staining was used to detect the apoptotic effects of CT in HFLS‑RA and MH7A cells. Flow cytometry was performed to detect the apoptotic and reactive oxygen species (ROS) levels induced by CT in HFLS‑RA cells...
December 3, 2018: International Journal of Molecular Medicine
Viktor S Simons, Guenter Lochnit, Jochen Wilhelm, Bernd Ishaque, Markus Rickert, Juergen Steinmeyer
Collagen hydrolysates (CHs) are heterogeneous mixtures of collagen peptides that are often used as nutraceuticals for osteoarthritis (OA). In this study, we compared the peptide composition and pharmacological effects of three different CH preparations (CH-Alpha® , Peptan® B 2000 and Mobiforte® ) as well as their production batches. Our biochemical analysis using MALDI-TOF mass spectrometry and the ICPL™-isotope labelling method revealed marked differences between different CH preparations and even between some production batches of the same preparation...
December 7, 2018: Scientific Reports
Jing Wu, Fu-Tao Zhao, Kai-Jian Fan, Jun Zhang, Bing-Xing Xu, Qi-Shan Wang, Ting-Ting Tang, Ting-Yu Wang
Dihydromyricetin (DMY), the main flavonoid of Ampelopsis grossedentata, has a potent anti-inflammation activity. However, the effect of DMY on the chronic autoimmune arthritis remains undefined. In this study we investigated the therapeutic effects of DMY on collagen-induced arthritis (CIA). Wistar rats were immunized with bovine type II collagen to establish CIA and then administered intraperitoneally (i.p.) with DMY (5, 25 and 50 mg/kg) every other day for five weeks. Paw swelling, clinical scoring and histologic analysis were performed to determine the therapeutic effects of DMY on arthritis development in CIA rats...
December 10, 2018: Journal of Pharmacology and Experimental Therapeutics
Taorong Wang, Gaoyuan Wang, Ye Zhang, Junqiang Zhang, Wei Cao, Xiaoyu Chen
Fibroblast-like synoviocytes in rheumatoid arthritis (RA-FLSs) play a key role in cartilage destruction. We previously found that resveratrol (Res) could promote FLSs apoptosis in adjuvant arthritis rats, but the underlying mechanism was unclear. According to our latest study, Res can suppress the expression of mitochondrial superoxide dismutase (MnSOD) and RA-FLSs proliferation. It was associated with elevated mitochondrial reactive oxygen species levels. Therefore, we hypothesized that Res-mediated RA-FLSs apoptosis might occur via the MnSOD- mitochondrial reactive oxygen species pathway...
December 4, 2018: European Journal of Pharmacology
Wenting Liao, Jiadong Sun, Wenjing Liu, Wenyu Li, Jiaxin Jia, Farong Ou, Kai Su, Youhua Zheng, Zhiguang Zhang, Yangpeng Sun
Histone deacetylases (HDACs) are important in chronic inflammation, and inflammatory responses affect synovium-derived mesenchymal stem cell (SMSC) function in temporomandibular joint repair. However, the effect of HDACs on SMSC inflammatory activation remains unclear. In this study, temporomandibular joint fibroblast-like synoviocytes obtained from osteoarthritis patients met the minimal mesenchymal stem cell criteria. Interleukin 1β (IL-1β) upregulated IL-6 and IL-8 expression in SMSCs through nuclear factor-κB (NF-κB) pathway activation...
December 4, 2018: Journal of Cellular Physiology
Ryoji Fujii, Rie Komatsu, Tomoo Sato, Iwao Seki, Koji Konomi, Hiroyuki Aono, Hisateru Niki, Kazuo Yudoh, Kusuki Nishioka, Toshihiro Nakajima
This study was performed to elucidate the molecular function of the synoviocyte proliferation-associated in collagen-induced arthritis (CIA) 1/serum amyloid A-like 1 (SPACIA1/SAAL1) in mice CIA, an animal model of rheumatoid arthritis (RA), and human RA-synovial fibroblasts (RASFs). SPACIA1/SAAL1 -deficient mice were generated and used to create mouse models of CIA in mild or severe disease conditions. Cell cycle-related genes, whose expression levels were affected by SPACIA1/SAAL1 small interfering RNA (siRNA), were screened...
November 30, 2018: International Journal of Molecular Sciences
Xiaofeng Guo, Dongmei Zhang, Xing Zhang, Jiawei Jiang, Pengfei Xue, Chunshuai Wu, Jinlong Zhang, Guohua Jin, Zhen Huang, Jian Yang, Xinhui Zhu, Wei Liu, Guanhua Xu, Zhiming Cui, Guofeng Bao
Fibroblast-like synoviocytes (FLSs) play an essential role in rheumatoid arthritis (RA) by promoting synovitis, pannus growth and cartilage/bone destruction. Increased proliferation, migration and invasion of FLSs greatly contribute to RA initiation and progression. Dual-specificity tyrosine-regulated kinase 1A (Dyrk1A), a serine/threonine kinase, regulates MAPK pathway activation, and governs the proliferation and differentiation of neuronal progenitor cells and cancer cells. Till now, the expression and possible function of Dyrk1A in RA FLSs have not been explored...
December 2018: Tissue & Cell
Yu Li, Jian-Zhong Xu, Chen-Xi Gu, Guan-Lei Liu, Ke Tian
BACKGROUND: Fibroblast-like synoviocytes (FLSs) play an essential role in the chronic inflammatory process of rheumatoid arthritis (RA). Carvacrol is a natural monoterpenic phenol that retains significant anti-inflammatory activity. However, the effect of carvacrol on inflammatory response in RA-FLSs has not yet been reported. The present study aimed to investigate the role of carvacrol in lipopolysaccharides (LPS)-induced inflammatory response in human RA-FLSs. METHODS: Cell viability and proliferation were measured by MTT and Cell Counting Kit-8 assays, respectively...
November 28, 2018: Journal of Cellular Biochemistry
Changzhi Song, Xiaozu Xu, Ya Wu, Biao Ji, Xiaoye Zhou, Ling Qin
Osteoarthritis (OA) is a degenerative joint disease characterized by the degradation of joint cartilage, the formation of osteophyma at joint margins, and synovial changes. Whereas lesions of the joint cartilage were the key point of the research and treatment of osteoarthritis before, a recent study showed that the synovium plays a crucial role in the pathological progress of OA. The inflammatory environment in the joints of OA patients always results in the overactivation of fibroblast-like synoviocytes (FLSs), which produce a multitude of inflammatory factors and media, not only leading to the degradation and injury of the cartilage tissue and promoting the development of osteoarthritis but also resulting in synovial fibrosis and joint stiffness...
November 27, 2018: Molecular Biology Reports
Kui Yang, Qin Yin, Qingcheng Mao, Sheng Dai, Lin Wang, Jiyang Dong, Jian Zuo
α-Mangostin (MAN) is a bioactive compound isolated from pericarp of mangosteen (Garcinia mangostana Linn.) with significant anti-rheumatic potentials. The purpose of this study was to explore the mechanisms underlying its therapeutic effects on collagen-induced arthritis (CIA) in rats with metabolomics approaches. Therapeutic effects of MAN on CIA were assessed by radiographic, histological, and immunohistochemical methods. Metabolic profiles of rats were characterized based on UPLC-MS/MS analysis of urine samples, followed by verification in HFLS-RA cells using a variety of toxicological and biochemical assays...
November 27, 2018: Inflammation
Le Chang, Xin Feng, Wei Gao
Fibroblast-like synoviocytes (FLSs), with their tumor-like proliferation, play an important role in rheumatoid arthritis (RA), and interleukin-17 (IL-17) participates in RA pathology by affecting FLSs. The aims of this study were to investigate the effects of IL-17 on the proliferation and autophagy of FLSs and the role of signal transducer and activator of transcription-3 (STAT3) in RA. FLSs were treated with IL-17 at different concentrations (0, 1, 10, and 20 ng/mL); then, autophagy was assayed with western blotting, immunofluorescence and transmission electron microscopy...
November 26, 2018: Connective Tissue Research
Peian Cai, Tongmeng Jiang, Bo Li, Xiong Qin, Zhenhui Lu, Yiguan Le, Chong Shen, Yifeng Yang, Li Zheng, Jinmin Zhao
The purpose of the present study was to investigate the underlying molecular mechanism of osteoarthritis (OA) and rheumatoid arthritis (RA) based on microarray profiles. Three human joint fibroblast-like synoviocytes (FLSs) microarray profiles including 26 OA samples, 33 RA samples, and 20 healthy control (HC) samples were downloaded from the GEO database. Differentially expressed genes (DEGs) between OA and HC (DEGsOA) and RA and HC (DEGsRA) were identified. Co-expressed and specific genes were analysed between DEGsOA and DEGsRA...
November 23, 2018: Cell Biochemistry and Function
Siqi Zhou, Haiyan Wen, Weisong Cai, Yubiao Zhang, Haohuan Li
Hypoxia/reoxygenation (H/R) plays an important role in the pathogenesis of osteoarthritis. Fibroblast-like synoviocytes (FLS), which are highly sensitive to H/R, are thought to be associated with cartilage degradation during osteoarthritis development. In this study, we investigated the biological effects of insulin-like growth factor (IGF) system and the expression of inflammatory mediators in FLS. We also pretreated FLS with tumor necrosis factor-α (TNF-α) before H/R in order to observe the response of FLS with the background of inflammatory cytokines...
November 19, 2018: Biochemical and Biophysical Research Communications
Oliver Frey, Marion Hückel, Mieczyslaw Gajda, Peter K Petrow, Rolf Bräuer
BACKGROUND: Fibroblast-like synoviocytes (FLSs) from patients with rheumatoid arthritis (RA) are autonomously activated to maintain inflammation and joint destruction in co-transplantation models. To elucidate inducing mechanisms involved in this altered behavior, the arthritogenic potential of FLSs from murine antigen-induced arthritis (AIA) were investigated in a transfer model. METHODS: FLSs were isolated, expanded in vitro, and transferred into knee joint cavities of severe combined immunodeficient (SCID) mice...
November 22, 2018: Arthritis Research & Therapy
David Bar-Or, Gregory Thomas, Leonard T Rael, Elizabeth Frederick, Melissa Hausburg, Raphael Bar-Or, Edward Brody
The low molecular weight fraction of commercial human serum albumin (LMWF5A) has been shown to successfully relieve pain and inflammation in severe osteoarthritis of the knee (OAK). LMWF5A contains at least three active components that could account for these anti-inflammatory and analgesic effects. We summarize in vitro experiments in bone marrow-derived mesenchymal stem cells, monocytic cell lines, chondrocytes, peripheral blood mononuclear cells, fibroblast-like synoviocytes, and endothelial cells on the biochemistry of anti-inflammatory changes induced by LMWF5A...
November 19, 2018: Current Rheumatology Reviews
Maria Sole Chimenti, Flavia Sunzini, Laura Fiorucci, Elisabetta Botti, Giulia Lavinia Fonti, Paola Conigliaro, Paola Triggianese, Luisa Costa, Francesco Caso, Alessandro Giunta, Maria Esposito, Luca Bianchi, Roberto Santucci, Roberto Perricone
Psoriasis (PsO) is an autoimmune disease characterized by keratinocyte proliferation, chronic inflammation and mast cell activation. Up to 42% of patients with PsO may present psoriatic arthritis (PsA). PsO and PsA share common pathophysiological mechanisms: keratinocytes and fibroblast-like synoviocytes are resistant to apoptosis: this is one of the mechanism facilitating their hyperplasic growth, and at joint level, the destruction of articular cartilage, and bone erosion and/or proliferation. Several clinical studies regarding diseases characterized by impairment of cell death, either due to apoptosis or necrosis, reported cytochrome c release from the mitochondria into the extracellular space and finally into the circulation...
2018: Frontiers in Immunology
Junxian Ma, Xinbo Wang, Tingting Lv, Jie Liu, Ying Ren, Jinshan Zhang, Yan Zhang
Ghrelin is a circulating peptide hormone, which involved in promoting feeding and regulating energy metabolism in human and rodents. Abnormal synovial hyperplasia is the most important pathologic hallmark of rheumatoid arthritis (RA), which is characterised by tumor-like expansion. Existing studies indicated that there may exist some relation between the decreased ghrelin and the abnormally proliferating synovial cells in RA. Therefore, the aim of this study is to explore the apoptotic effects of ghrelin on MH7A synovial cells in vitro...
November 15, 2018: Biological & Pharmaceutical Bulletin
Xiaodong Wen, Xun Chen, Xiaojun Liang, Hongmou Zhao, Yi Li, Xiangxiang Sun, Jun Lu
The activation of TNF-α/NF-кB signaling is involved in the regulation of a wide range of biological processes, such as cell proliferation, differentiation and apoptosis, eventually causing a number of diseases, such as cancer and inflammation. Here, we found that TNF-α/NF-кB signaling was activated in a large number of blood samples taken from foot and ankle rheumatoid arthritis (RA) patients. By applying a microarray assay to the human synovial sarcoma cell line SW982 and the human fibroblast-like synoviocyte cell line HFLS-RA, as well as in their corresponding p65 knockdown and -overexpressing cells, we identified and verified the activation of many p65 targets, including cytokines (e...
2018: International Journal of Biological Sciences
Ankit Mahendra, Xingyu Yang, Shaza Abnouf, Jay R T Adolacion, Daechan Park, Sanam Soomro, Jason Roszik, Cristian Coarfa, Gabrielle Romain, Keith Wanzeck, S Louis Bridges, Amita Aggarwal, Peng Qiu, Sandeep K Agarwal, Chandra Mohan, Navin Varadarajan
OBJECTIVE: To obtain the comprehensive transcriptome profile of human citrulline-specific B cells from patients diagnosed with rheumatoid arthritis (RA). METHODS: Citrulline and hemagglutinin (HA) specific B cells were flow-sorted using peptide-streptavidin conjugates from peripheral blood of RA patients and healthy individuals respectively. Transcriptome profile of the sorted cells was obtained by RNA-sequencing, and expression of key protein molecules was evaluated by aptamer-based SOMAscan assay and flow cytometry...
November 8, 2018: Arthritis & Rheumatology
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