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Medical Parasitology

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https://www.readbyqxmd.com/read/30094540/lyophilisation-as-a-simple-and-safe-method-for-long-term-storage-of-free-living-amoebae-at-ambient-temperature
#1
Carsten Balczun, Patrick L Scheid
Free-living amoebae (FLA) are protozoa ubiquitously found in nature. As some species or strains of these FLA are pathogenic for humans and animals, they represent objects of medical and parasitological research worldwide. Storage of valuable FLA strains in laboratories is often time- and energy-consuming and expensive. The shipment of such strains as frozen stocks is cumbersome and challenging in terms of cooling requirements as well as of transport regulations. To overcome these difficulties and challenges in maintenance and transport, we present a new method to generate lyophilised samples of non-cyst-forming FLA (Ripella (Vannella) spp...
August 9, 2018: Parasitology Research
https://www.readbyqxmd.com/read/29983512/prevalence-of-opportunistic-intestinal-parasites-and-associated-factors-among-hiv-patients-while-receiving-art-at-arba-minch-hospital-in-southern-ethiopia-a-cross-sectional-study
#2
Getaneh Alemu, Dagninet Alelign, Ashenafi Abossie
Background: Opportunistic Intestinal Parasites (OIPs) commonly cause morbidity in HIV/AIDS patients due to the decline of CD4+ T-cells. The burden of Opportunistic Intestinal Parasitic Infections (OIPIs) in Ethiopia is expected to be high due to poor sanitation and co-pandemicity of HIV/AIDs. Therefore, frequent assessment of the magnitude and associated factors for intestinal parasitosis is essential for the management of HIV/AIDS patients. Methods: A hospital based cross-sectional study was conducted among patients attending Arba Minch Hospital Antiretroviral Therapy (ART) Clinic from March to June 2016...
March 2018: Ethiopian Journal of Health Sciences
https://www.readbyqxmd.com/read/29963088/induction-of-apoptosis-by-alcoholic-extract-of-combination-verbascum-thapsus-and-ginger-officinale-on-iranian-isolate-of-trichomonas-vaginalis
#3
Zohreh Fakhrieh-Kashan, Mohsen Arbabi, Mahdi Delavari, Mahdi Mohebali, Hossein Hooshyar
Background: The protozoan Trichomonas vaginalis is a sexually transmitted disease (STD). Metronidazole is a chosen drug for the treatment. This study evaluated the anti trichomonal activity of alcoholic extracts of combination Verbascum thapsus and Ginger officinale. Methods: This experimental study was conducted in the Parasitology Laboratory, Kashan University of Medical Sciences, Kashan, Iran in 2015, on 23 women with suspected trichomoniasis referring to Kashan clinical centers...
January 2018: Iranian Journal of Parasitology
https://www.readbyqxmd.com/read/29922619/molecular-evidence-of-human-fasciolosis-due-to-fasciola-gigantica-in-iran-a-case-report
#4
Mohammad Bagher Rokni, Arezoo Bozorgomid, Peyman Heydarian, Mojgan Aryaeipour
Fascioliasis is a foodborne zoonotic disease caused by the two parasite species Fasciola hepatica and F. gigantica . In spite of the presence of both species of Fasciola in the livestock, to our knowledge, to date, no cases of human F. gigantica infection have been reported in Iran officially. Here, we report such a case in a 25 yr old woman referred to The Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran in 2016. CT imaging and MRCP revealed an ill-defined lesion of segments of liver...
May 2018: Iranian Journal of Public Health
https://www.readbyqxmd.com/read/29914556/occurrence-of-health-compromising-protozoan-and-helminth-infections-in-tortoises-kept-as-pet-animals-in-germany
#5
Malek J Hallinger, Anja Taubert, Carlos Hermosilla, Frank Mutschmann
BACKGROUND: Exotic reptiles such as tortoises, have become increasingly common domestic pets worldwide and are known to host different gastrointestinal parasites. Some of these parasites bear zoonotic potential. In the present survey, we parasitologically examined tortoise faecal samples (n = 1005) from 19 different species held as pets in private German households and German zoological gardens. METHODS: Saline faecal smears were used to generate prevalence data for potentially health-compromising gastrointestinal parasites...
June 18, 2018: Parasites & Vectors
https://www.readbyqxmd.com/read/29753336/an-important-milestone-in-parasitology-celebrating-a-hundred-volumes-of-advances-in-parasitology
#6
J Russell Stothard, David Rollinson
Beginning in 1963, the founding rationale of Advances in Parasitology was to provide authentic, well-documented reviews by leading experts, about the progress being made in their area of specialism to inform the wider cadre of parasitologists, disseminating this information across allied disciplines and all users. Some 55 years later, the Series has accumulated over 667 published articles, with just over 650 authors contributing either alone or in collaboration, and has successfully served the parasitological needs of medical, veterinary and wildlife scientific communities with equity, notwithstanding treatises on vectors or intermediate hosts, as well as 'honorary parasites' such as viruses, bacteria and fungi...
2018: Advances in Parasitology
https://www.readbyqxmd.com/read/29665876/diversity-distribution-and-parasitism-rates-of-fleas-insecta-siphonaptera-on-sigmodontine-rodents-cricetidae-from-argentinian-patagonia
#7
J Sanchez, M Lareschi
Fleas have great medical relevance as vectors of the causative agents of several diseases in animals and humans and rodents are the principal reservoirs for these pathogens. Argentinian Patagonia has the highest diversity of rodent fleas in South America. However, parasitism rates of rodents by fleas, the factors that influence them and the ecological aspects that modulate geographical distributions of flea-host association remain unknown for this region. This is the first study to record the diversity, prevalence, abundance, geographical distributions and host ranges of fleas in Argentinian Patagonia...
April 18, 2018: Bulletin of Entomological Research
https://www.readbyqxmd.com/read/29606364/pyronaridine-artesunate-or-dihydroartemisinin-piperaquine-versus-current-first-line-therapies-for-repeated-treatment-of-uncomplicated-malaria-a-randomised-multicentre-open-label-longitudinal-controlled-phase-3b-4-trial
#8
(no author information available yet)
BACKGROUND: Artemether-lumefantrine and artesunate-amodiaquine are used as first-line artemisinin-based combination therapies (ACTs) in west Africa. Pyronaridine-artesunate and dihydroartemisinin-piperaquine are potentially useful for diversification of ACTs in this region, but further safety and efficacy data are required on malaria retreatment. METHODS: We did a randomised, multicentre, open-label, longitudinal, controlled phase 3b/4 clinical trial at seven tertiary centres in Burkina Faso, Guinea, and Mali...
April 7, 2018: Lancet
https://www.readbyqxmd.com/read/29601572/brugia-malayi-infection-in-ferrets-a-small-mammal-model-of-lymphatic-filariasis
#9
Belinda M Jackson-Thompson, So Young Kim, Shalini Jaiswal, Jessica R Scott, Scott R Jones, C Paul Morris, J Judd Fite, Karen Laurie, Andrew R Hoy, Bernard J Dardzinski, Edward Mitre
BACKGROUND: The lack of effective short-course therapies for treatment of the adult stage of filarial worms is a major limitation in the global effort to eliminate lymphatic filariasis. Studies using current small mammal models of lymphatic filariasis are limited by difficulties in quantifying adult worm numbers and in assessing lymphatic anatomy and function. METHODOLOGY/PRINCIPAL FINDINGS: Here, we re-established Brugia malayi infection of ferrets as a model for lymphatic filariasis and demonstrated parasitological, immunological, and histological parallels with human infection...
March 2018: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/29498738/blastocystis-hominis-transmission-by-non-potable-water-a-case-report-in-italy
#10
Maria Cristina Angelici, Chiara Nardis, Riccardo Scarpelli, Paola Ade
In the reported case, a 41-year-old Italian man came to the clinician's observation reporting cramps, bloating and watery diarrhoea a few days after drinking water indicated as unpotable from a fountain in a farm area. The medical suspicion was directed at both gluten intolerance and enteric infection, eventually of waterborne origin. Gluten intolerance was investigated by intestinal biopsy and excluded, while stool analyses ruled out infective bacteriological or viral agents and parasites. Subsequently, a persistent eosinophilia was revealed and a parasitological analysis was again suggested, planning for a more sensitive molecular method...
April 2018: New Microbiologica
https://www.readbyqxmd.com/read/29469536/-experience-and-analysis-of-excellent-works-of-human-parasitology-in-national-medical-colleges-and-universities
#11
Yang Xiao-di, Fang Qiang, Cheng Yang, Chang Xue-Lian, Tao Zhi-Yong, Wang Yuan-Yuan, Wang Xiao-Li, Li Jiang-Yan, Huang Shi-Ya, Jiao Yu-Meng, Wang Xue-Mei, Xia Hui, Chen Xing-Zhi
Teaching competition is an effective way for college and university teachers to improve their teaching skills. Based on the teaching practice and experience in medical parasitology, this paper discusses several key issues in teaching competition including topics, teaching designs and teaching methods. It provides references for the teachers in department of parasitology of universities and colleges to improve the quality of classroom teaching.
May 17, 2017: Zhongguo Xue Xi Chong Bing Fang Zhi za Zhi, Chinese Journal of Schistosomiasis Control
https://www.readbyqxmd.com/read/29440898/nanosilver-new-ageless-and-versatile-biomedical-therapeutic-scaffold
#12
REVIEW
Shahid Ullah Khan, Tawfik A Saleh, Abdul Wahab, Muhammad Hafeez Ullah Khan, Dilfaraz Khan, Wasim Ullah Khan, Abdur Rahim, Sajid Kamal, Farman Ullah Khan, Shah Fahad
Silver nanotechnology has received tremendous attention in recent years, owing to its wide range of applications in various fields and its intrinsic therapeutic properties. In this review, an attempt is made to critically evaluate the chemical, physical, and biological synthesis of silver nanoparticles (AgNPs) as well as their efficacy in the field of theranostics including microbiology and parasitology. Moreover, an outlook is also provided regarding the performance of AgNPs against different biological systems such as bacteria, fungi, viruses, and parasites (leishmanial and malarial parasites) in curing certain fatal human diseases, with a special focus on cancer...
2018: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/29318992/cutaneous-leishmaniasis-in-pediatric-patients-in-a-single-tertiary-hospital-in-ankara
#13
Ayşe Kaman, Gönül Tanır, Zeynep Gökçe Gayretli Aydın, Özge Metin, Türkan Aydın Teke, Fatma Nur Öz, Mesut Mungan
OBJECTIVE: Leishmaniasis is an infectious disease that is caused by a protozoan parasite of the Leishmania genus and that occurs worldwide. Leishmaniasis is endemic in southeastern Turkey and the neighboring Middle Eastern countries. The purpose of this study was to describe the clinical characteristics of patients admitted to our hospital with a diagnosis of cutaneous leishmaniasis (CL). METHODS: A total of 16 CL patients [11 (69%) boys and five (31%) girls] were admitted between January 2014 and December 2015...
December 2017: Türkiye Parazitolojii Dergisi
https://www.readbyqxmd.com/read/29290581/first-molecular-detection-of-babesia-canis-in-dogs-from-bosnia-and-herzegovina
#14
Agnesa Ćoralić, Simona Gabrielli, Amir Zahirović, Nikola M Stojanović, Giovanni Luigi Milardi, Adnan Jažić, Almedina Zuko, Denis Čamo, Suzana Otašević
Babesia spp. are tick-transmitted protozoan haemoparasites of great economic, veterinary and medical impact worldwide. Herein we reported the very high prevalence of autochthonous babesiosis in symptomatic dogs from Bosnia and Herzegovina in the period from 2014 to 2016. Eighty dogs that did not leave the country were examined using parasitological and molecular analyses and babesiosis was diagnosed in 82.5% and 85.0% of them, respectively (p < 0.001). One species, Babesia canis was identified using molecular methodology (PCR and sequence analysis)...
February 2018: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/29281988/intravenous-artesunate-plus-artemisnin-based-combination-therapy-act-or-intravenous-quinine-plus-act-for-treatment-of-severe-malaria-in-ugandan-children-a-randomized-controlled-clinical-trial
#15
RANDOMIZED CONTROLLED TRIAL
Pauline Byakika-Kibwika, Jane Achan, Mohammed Lamorde, Carine Karera-Gonahasa, Agnes N Kiragga, Harriet Mayanja-Kizza, Noah Kiwanuka, Sam Nsobya, Ambrose O Talisuna, Concepta Merry
BACKGROUND: Severe malaria is a medical emergency associated with high mortality. Adequate treatment requires initial parenteral therapy for fast parasite clearance followed by longer acting oral antimalarial drugs for cure and prevention of recrudescence. METHODS: In a randomized controlled clinical trial, we evaluated the 42-day parasitological outcomes of severe malaria treatment with intravenous artesunate (AS) or intravenous quinine (QNN) followed by oral artemisinin based combination therapy (ACT) in children living in a high malaria transmission setting in Eastern Uganda...
December 28, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/29260753/-clinical-and-epidemiological-features-of-visceral-leishmaniasis-in-the-republic-of-crimea
#16
M S Baranets, T N Ermak, E N Ponirovsky
The paper analyzes official statistical data, as well as visceral leishmaniasis (VL) cases who have visited the Clinical Center, I.M. Sechenov First Moscow State Medical University, and the results of parasitological studies of the materials submitted to the Reference Center for Monitoring of Leishmaniasis, E.I. Martsinovsky Institute of Medical Parasitology, Tropical and Vector-Borne Diseases. Account is taken of the observations made by the Central Research Institute of Epidemiology, Russian Federal Service for Supervision of Consumer Rights Protection and Human Welfare, and of the literature data available in Russian and foreign (Ukraine) scientific journals and monographs...
2017: Terapevticheskiĭ Arkhiv
https://www.readbyqxmd.com/read/29216181/case-report-a-rare-case-of-urinary-myiasis-induced-by-the-fourth-instar-larvae-of-telmatoscopus-albipunctatus
#17
Beibei Zhang, Lifu Wang, Jiahua Liu, Lian Xu, Langui Song, Xiaoying Wu, Xi Sun, Zhongdao Wu
Telmatoscopus albipunctatus, a cosmopolitan fly, is widely distributed throughout moist environments. It is one of the most medically important insects (especially in urban environments) that may potentially cause myiasis. Urinary myiasis and other sites of infestation, including the intestine, nasal passages, lung, and derma, have been reported. This is the first case report of a Chinese middlescent woman infected with T. albipunctatus in Guangzhou, China. In the present report, a 50-year-old woman came to The Third Affiliated Hospital of Southern Medical University, Guangzhou, China, because larvae were found when urinating in the morning; this had occurred every two days within the past two months...
December 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/29196277/clinical-and-epidemiological-features-of-chronic-trypanosoma-cruzi-infection-in-patients-with-hiv-aids-in-buenos-aires-argentina
#18
Andrés Guillermo Benchetrit, Marisa Fernández, Amadeo Javier Bava, Marcelo Corti, Norma Porteiro, Liliana Martínez Peralta
OBJECTIVES: Trypanosoma cruzi reactivation in HIV patients is considered an opportunistic infection, usually with a fatal outcome. The aim of this study was to describe the epidemiological and clinical features of T. cruzi infection in HIV patients and to compare these findings between patients with and without Chagas disease reactivation. METHODS: The medical records of T. cruzi-HIV co-infected patients treated at the Muñiz Infectious Diseases Hospital from January 2005 to December 2014 were reviewed retrospectively...
February 2018: International Journal of Infectious Diseases: IJID
https://www.readbyqxmd.com/read/29155816/liposomal-amphotericin-b-in-travelers-with-cutaneous-and-muco-cutaneous-leishmaniasis-not-a-panacea
#19
Romain Guery, Benoit Henry, Guillaume Martin-Blondel, Claire Rouzaud, Florence Cordoliani, Gundel Harms, Jean-Pierre Gangneux, Françoise Foulet, Emmanuelle Bourrat, Michel Baccard, Gloria Morizot, Paul-Henri Consigny, Antoine Berry, Johannes Blum, Olivier Lortholary, Pierre Buffet
BACKGROUND: Complex cutaneous and muco-cutaneous leishmaniasis (CL and MCL) often requires systemic therapy. Liposomal amphotericin B (L-AmB) has a strong potential for a solid clinical benefit in this indication. METHODS: We conducted a retrospective analysis of data from a French centralized referral treatment program and from the "LeishMan" European consortium database. All patients with parasitologically proven CL or MCL who received at least one dose of L-AmB were included...
November 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/29140309/biosensors-to-diagnose-chagas-disease-a-brief-review
#20
REVIEW
María-Isabel Rocha-Gaso, Luis-Jesús Villarreal-Gómez, Denis Beyssen, Frédéric Sarry, Marco-Antonio Reyna, Carlos-Napoleón Ibarra-Cerdeña
Chagas disease (CD), which mostly affects those living in deprived areas, has become one of Latin America's main public health problems. Effective prevention of the disease requires early diagnosis, initiation of therapy, and regular blood monitoring of the infected individual. However, the majority of the Trypanosoma cruzi infections go undiagnosed because of mild symptoms, limited access to medical attention and to a high variability in the sensitivity and specificity of diagnostic tests. Consequently, more affordable and accessible detection technologies capable of providing early diagnosis and T...
November 15, 2017: Sensors
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