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Eric Miller, Amit Vikram, Getahun E Agga, Terrance M Arthur, John W Schmidt
Approximately 20% of U.S. beef cattle receive prophylactic in-feed administration of chlortetracycline (CTC) to reduce bovine respiratory disease (BRD) incidence during the transition into feedlots. To determine the impact of prophylaxis on selected antimicrobial resistance genes (ARGs), 300 beef cattle were placed into 10 pens (30 head/pen). Five "CTC group" pens received in-feed CTC (10 mg/lb of body weight/d) from the fifth to ninth day after feedlot arrival, whereas the five "Control group" pens received no CTC...
August 15, 2018: Foodborne Pathogens and Disease
Manuel Garcia-Algar, Ana Fernandez-Carrascal, Ana Olano-Daza, Luca Guerrini, Neus Feliu, Wolfgang J Parak, Roger Guimera, Eduardo Garcia-Rico, Ramon A Alvarez-Puebla
In this manuscript, we demonstrate the applicability of a metabolic liquid biopsy for the monitoring and staging of patients with lung cancer. This method provides an unbiased detection strategy to establish a more precise correlation between CTC quantification and the actual burden of disease, therefore improving the accuracy of staging based on current imaging techniques. Also, by applying statistical analysis techniques and probabilistic models to the metabolic status and distribution of peripheral blood mononuclear cell (PBMC) populations "perturbed" by the presence of CTCs, a new category of adaptive metabolic pattern biomarker (AMPB) is described and unambiguously correlated to the different clinical stages of the patients...
2018: NPJ Precision Oncology
M Craig Miller, Peggy S Robinson, Christopher Wagner, Daniel J O'Shannessy
Cancer cells from solid tumors can enter the circulatory system and survive to subsequently form distant metastases. The CellSearch® system (Menarini-Silicon Biosystems, Huntingdon Valley, PA) was the first, FDA-cleared system that provided a reliable tool for the investigation of circulating tumor cells (CTCs), which have been shown to be strongly associated with poor survival and therapy failure. Since that time, a number of new technologies have been introduced to improve CTC detection and/or isolation for further characterization...
August 14, 2018: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
Takashi Ohnaga, Yoshinori Takei, Takuya Nagata, Yutaka Shimada
Epidermal growth factor receptor (EGFR) was evaluated as a target antigen for cancer cell capture by microfluidic methods based on antigen-antibody association. A polymer CTC-chip microfluidic device was surface-functionalized with three different anti-EGFR antibodies and used to capture EGFR-expressing cancer cells. Capture efficacy depended on the type of antibody used, and cetuximab efficiently captured cancer cell lines that had a wide range of EGFR expression. Capture efficiency was analyzed from the viewpoint of antigen-antibody association in a kinetic process, i...
August 13, 2018: Scientific Reports
Tanya T Kwan, Aditya Bardia, Laura M Spring, Anita Giobbie-Hurder, Mark Kalinich, Taronish Dubash, Tilak Sundaresan, Xin Hong, Joseph A LiCausi, Uyen Ho, Erin J Silva, Ben S Wittner, Lecia V Sequist, Ravi Kapur, David T Miyamoto, Mehmet Toner, Daniel A Haber, Shyamala Maheswaran
The multiplicity of new therapies for breast cancer presents a challenge for treatment selection. We describe a 17-gene digital signature of breast circulating tumor cell (CTC)-derived transcripts enriched from blood, enabling high-sensitivity early monitoring of response. In a prospective cohort of localized breast cancer, an elevated CTC-Score after three cycles of neoadjuvant therapy is associated with residual disease at surgery (p=0.047). In a second prospective cohort with metastatic breast cancer, baseline CTC-Score correlates with overall survival (p= 0...
August 13, 2018: Cancer Discovery
Joan Carles, Daniel Castellano, María-José Méndez-Vidal, Begoña Mellado, María-Isabel Saez, Aránzazu González Del Alba, José-Luis Perez-Gracia, José Jimenez, Cristina Suárez, Juan M Sepúlveda, Ray Manneh, Ignacio Porras, Cristina López, Rafael Morales-Barrera, José-Ángel Arranz
INTRODUCTION: Although increasing numbers of therapies with proven survival benefits have become available for metastatic castration-resistant prostate cancer (mCRPC), including radium-223, there is still a need for reliable biomarkers that provide information about clinically meaningful outcomes and treatment responses. MATERIALS AND METHODS: This study was a translational study that was conducted prospectively by the Spanish Oncology Genito-Urinary Group and included 45 patients with histologically confirmed mCRPC who were treated with radium-223...
July 21, 2018: Clinical Genitourinary Cancer
Benjamin J DiPardo, Paul Winograd, Colin M Court, James S Tomlinson
Pancreatic cancer (PC) is a highly lethal disease, in part because of early metastasis, late diagnosis, and limited treatment options. Circulating tumor cells (CTCs) are cancer cells that have achieved the metastatic step of intravasation, and are thus a unique source of biomarkers with potential applications in the staging, prognostication, and treatment of PC. Areas covered: This review describes the use of CTCs in PC, including isolation methods, the significance of CTC enumeration, and studies examining phenotypic and molecular characteristics of CTCs...
August 11, 2018: Expert Review of Molecular Diagnostics
Wan-Ming Li, Lin-Lin Zhou, Min Zheng, Jin Fang
Circulating tumor cells (CTCs) have the potential to predict metastasis, and the capture of CTCs based on their surface markers is mostly applied for CTC detection. Considering that the CTCs with a metastatic phenotype preferably form a metastatic focus and that aptamers have the ability to bind targets with high specificity and affinity, we selected aptamers directed toward metastatic cells by subtractive Cell-SELEX technology using highly metastatic MDA-MB-231 cells as the target cell and low-metastatic MCF-7 cells as the negative cell for the capture of metastatic CTCs...
July 19, 2018: Molecular Therapy. Nucleic Acids
Maryou Bk Lambros, George Seed, Semini Sumanasuriya, Veronica Gil, Mateus Crespo, Mariane Sousa Fontes, Rob Chandler, Niven Mehra, Gemma Fowler, Berni Ebbs, Penelope Ruth Flohr, Susana Miranda, Wei Yuan, Alan Mackay, Ana Ferreira, Rita Pereira, Claudia Bertan, Ines Figueiredo, Ruth Riisnaes, Daniel Nava Rodrigues, Adam Sharp, Jane Goodall, Gunther Boysen, Suzanne Carreira, Diletta Bianchini, Pasquale Rescigno, Zafeiris Zafeiriou, Joanne Hunt, Deirdre Moloney, Lucy Hamilton, Rui P L Neves, Joost F Swennenhuis, Kiki C Andree, Nikolas H Stoecklein, Leon Wmm Terstappen, Johann S de Bono
PURPOSE: Circulating tumor cells (CTCs) have clinical relevance, but their study has been limited by their low frequency. EXPERIMENTAL DESIGN: We evaluated liquid biopsies by apheresis to increase CTC yield from patients suffering from metastatic prostate cancer, allow precise gene copy number calls, and study disease heterogeneity. RESULTS: Apheresis was well-tolerated and allowed the separation of large numbers of CTCs; the average CTC yield from 7...
August 9, 2018: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
Junwei Song, Yan Zhao, Yingyi Zhang, Pingqing Fu, Lishan Zheng, Qi Yuan, Shan Wang, Xiaofeng Huang, Weihai Xu, Zhixiang Cao, Sergey Gromov, Senchao Lai
Total suspended particle (TSP) samples were collected during a cruise campaign over the western South China Sea (SCS) from August to September 2014. Ten water-soluble ions (WSI), organic carbon (OC), elemental carbon (EC) and stable carbon isotope ratios of total carbon (δ13 CTC ) were measured. The average concentrations of total WSI, OC and EC were 7.91 ± 3.44 μg/m3 , 2.04 ± 1.25 μg/m3 and 0.30 ± 0.22 μg/m3 , respectively. Among the investigated WSI, sulfate (SO4 2- ), sodium (Na+ ) and chloride (Cl- ) were the most abundant species, accounting for 39...
July 24, 2018: Environmental Pollution
Xuehui Zhang, Hui Li, Xiuyan Yu, Shanxin Li, Zhen Lei, Chang Li, Qun Zhang, Qing Han, Yuan Li, Kun Zhang, Yuxiang Wang, Congrong Liu, Yiqing Mao, Xi Wang, David M Irwin, Hongyan Guo, Gang Niu, Huanran Tan
BACKGROUND/AIMS: Monitoring the appearance and progression of tumors are important for improving the survival rate of patients with ovarian cancer. This study aims to examine circulating tumor cells (CTCs) in epithelial ovarian cancer (EOC) patients to evaluate their clinical significance in comparison to the existing biomarker CA125. METHODS: Immuomagnetic bead screening, targeting epithelial antigens on ovarian cancer cells, combined with multiplex reverse transcriptase-polymerase chain reaction (Multiplex RT-PCR) was used to detect CTCs in 211 samples of peripheral blood (5 ml) from 109 EOC patients...
August 9, 2018: Cellular Physiology and Biochemistry
Ling-Ling Wu, Man Tang, Zhi-Ling Zhang, Chu-Bo Qi, Jiao Hu, Xu-Yan Ma, Dai-Wen Pang
Profiling the heterogeneous phenotypes of single circulating tumor cells (CTCs) from patients is a very challenging task which paves new ways for cancer management, especially personalized anti-cancer therapy. Herein, we propose a chip-assisted multifunctional nanosphere system for efficient and reliable biomarker phenotype analysis of single heterogeneous CTCs. Red fluorescent-magnetic-biotargeting multifunctional nanospheres and green fluorescent-biotargeting nanospheres targeting to two kinds of CTC biomarkers, are used for convenient dual-fluorescence labeling of CTCs along with magnetic tags...
August 8, 2018: Analytical Chemistry
T M Morgan, X Wang, X Qian, J M Switchenko, S Nie, K R Patel, R J Cassidy, D M Shin, J J Beitler
OBJECTIVES: We report the outcomes of patients with squamous cell carcinoma of the head and neck (HNSCC) whose circulating tumor cells (CTCs) were quantified using surface-enhanced Raman scattering (SERS) nanotechnology. METHODS: SERS tagged with EGF was used to directly measure targeted CTCs. Patient charts were retrospectively reviewed. An optimal cut point for CTCs in 7.5 ml of peripheral blood predictive of for distant metastasis-free survival (DMFS) was identified by maximizing the log-rank statistic...
August 6, 2018: Clinical & Translational Oncology
Costanza Paoletti, Gaia Schiavon, Emily M Dolce, Elizabeth P Darga, Thomas H Carr, Joseph Geradts, Matthias Hoch, Teresa Klinowska, Justin P O Lindemann, Gayle Marshall, Shethah Morgan, Parul Patel, Vicky Rowlands, Nitharsan Sathiyayogan, Kimberly Aung, Erika P Hamilton, Manish R Patel, Anne C Armstrong, Komal Jhaveri, Seock-Ah Im, Nadia Iqbal, Fouziah Butt, Caroline Dive, Elizabeth A Harrington, J Carl Barrett, Richard D Baird, Daniel F Hayes
BACKGROUND: Common resistance mechanisms to endocrine therapy (ET) in estrogen receptor (ER) positive metastatic breast cancers include, among others, ER loss and acquired activating mutations in the ligand-binding domain of the ER gene ( ESR1LBD m ). ESR1 mutational mediated resistance may be overcome by selective ER degraders (SERDs). During the first-in-human study of oral SERD AZD9496, early changes in circulating tumor cells (CTC) and circulating tumor DNA (ctDNA) were explored as potential non-invasive tools, alongside paired tumor biopsies, to assess pharmacodynamics and early efficacy...
August 6, 2018: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
Diane Goéré, Jean-Pierre Pignon, Maximiliano Gelli, Dominique Elias, Léonor Benhaim, Frédéric Deschamps, Caroline Caramella, Valérie Boige, Michel Ducreux, Thierry de Baere, David Malka
BACKGROUND: After curative-intent surgery for colorectal liver metastases (CRLM), liver recurrence occurs in more than 60% of patients, despite the administration of perioperative or adjuvant chemotherapy. This risk is even higher after resection of more than three CRLM. As CRLM are mostly supplied by arterial blood flow, hepatic arterial infusion (HAI) of chemotherapeutic agents after resection of CRLM is an attractive approach. Oxaliplatin-based HAI chemotherapy, in association with systemic fluoropyrimidines, has been shown to be safe and highly active in patients with CRLM...
August 6, 2018: BMC Cancer
Margret Ehlers, Stephanie Allelein, Franziska Schwarz, Hubertus Hautzel, Anne Kuebart, Mathias Schmidt, Matthias Haase, Till Dringenberg, Matthias Schott
Circulating tumor cells (CTCs) have been shown to be a valuable prognostic marker for different solid cancers. Within the present study we quantified CTCs in thyroid cancer (TC) patients. Special focus was given to disease-free PTC patients with undetectable serum thyroglobulin (Tg) levels. Altogether, 67 TC patients (33 papillary, 20 follicular, 14 medullary) were included in the study. CTC numbers, which were normalized to 3.3×105 peripheral blood mononuclear cells, were correlated with clinical outcome...
August 2018: Hormone and Metabolic Research, Hormon- und Stoffwechselforschung, Hormones et Métabolisme
Hongbo Chen, Yefeng Zhou, Xiayi Hu, Ke Tian, Junfeng Zhang
Due to the widespread use of antibiotics in healthcare and livestock production, antibiotic resistance genes and residual antimicrobials would enter environment and further discharge into the municipal sewage system. The objective of this work was to explore the potential effect of chlortetracycline (CTC) on biological nutrient removal from wastewater. Thus, the effects of CTC on biological phosphorus and nitrogen removal were investigated with respect to the viability of bacteria, the activities of key metabolic enzymes, and the transformations of intermediate metabolites...
August 1, 2018: Science of the Total Environment
Hillary Ganek, Ron Smyth, Stephanie Nixon, Alice Eriks-Brophy
Purpose: This study investigates how the variables of culture and hearing status might influence the amount of parent-child talk families engage in throughout an average day. Method: Seventeen Vietnamese and 8 Canadian families of children with hearing loss and 17 Vietnamese and 13 Canadian families with typically hearing children between the ages of 18 and 48 months old participated in this cross-comparison design study. Each child wore a Language ENvironment Analysis system digital language processor for 3 days...
August 3, 2018: Journal of Speech, Language, and Hearing Research: JSLHR
Dennis Woo, Min Yu
Circulating tumor cells (CTCs) are a subset of cancer cells that are shed from the primary or metastatic tumors into the bloodstream. CTCs are responsible for the establishment of blood-borne distant metastases but their rarity, estimated at one CTC per billion blood cells, presents the biggest technical barrier to their functional studies. Recent advances in CTC isolation technology have allowed for the reliable capture of CTCs from the whole blood of cancer patients. The ability to derive clinically relevant information from CTCs isolated through a blood draw allows for the monitoring of active disease, avoiding the invasiveness inherent to traditional biopsy techniques...
July 17, 2018: Translational Research: the Journal of Laboratory and Clinical Medicine
Sathya Neelature Sriramareddy, Etienne Hamoir, Marcela Chavez, Renaud Louis, Albert Beckers, Luc Willems
Early detection of tumor relapse is a major issue in patients with medullary thyroid carcinoma. Calcitonin has been reported as a sensitive and accurate marker for recurrence of medullary thyroid carcinoma after thyroidectomy. Recent evidence nevertheless reveals pitfalls in calcitonin immunoassays due to the presence of heterophilic antibodies or macroaggregates. Calcitonin can also remain undetectable despite metastasis of rare tumor cells in thyroidectomized patients. In this context, we designed a sensitive and specific technique to identify calcitonin-positive circulating tumor cells (CTC) in medullary thyroid carcinoma...
August 2, 2018: Endocrine-related Cancer
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