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Protein Corona

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https://www.readbyqxmd.com/read/30336593/physicochemical-characterization-of-fret-labelled-chitosan-nanocapsules-and-model-degradation-studies
#1
Stefan Hoffmann, Christian Gorzelanny, Bruno Moerschbacher, Francisco M Goycoolea
Sub-micron o/w emulsions coated with chitosan have been used for drug delivery, quorum sensing inhibition, and vaccine development. To study interactions with biological systems, nanocapsules have been fluorescently labelled in previous works, but it is often difficult to distinguish the released label from intact nanocapsules. In this study, we present advanced-labelling strategies based on Förster Resonance Energy Transfer (FRET) measurements for chitosan-coated nanocapsules and investigate their dissolution and degradation...
October 17, 2018: Nanomaterials
https://www.readbyqxmd.com/read/30334470/understanding-the-elusive-protein-corona-of-thermoresponsive-nanogels
#2
Enrico Miceli, Benno Kuropka, Christine Rosenauer, Ernesto R Osorio Blanco, Loryn E Theune, Mrityunjoy Kar, Christoph Weise, Svenja Morsbach, Christian Freund, Marcelo Calderón
AIM: We analyzed the protein corona of thermoresponsive, poly(N-isopropylacrylamide)- or poly(N-isopropylmethacrylamide)-based nanogels. MATERIALS & METHODS: Traces of protein corona detected after incubation in human serum were characterized by proteomics and dynamic light scattering in undiluted serum. RESULTS: Apolipoprotein B-100 and albumin were the main components of the protein coronae. For dendritic polyglycerol-poly(N-isopropylacrylamide) nanogels at 37°C, an increase in adsorbed immunoglobulin light chains was detected, followed by partially reversible nanogel aggregation...
October 18, 2018: Nanomedicine
https://www.readbyqxmd.com/read/30322787/prevalence-of-pica-in-patients-on-dialysis-and-its-association-with-nutritional-status
#3
Claudia N Orozco-González, Laura Cortés-Sanabria, Alfonso M Cueto-Manzano, Beatriz Corona-Figueroa, Héctor R Martínez-Ramírez, Jorge López-Leal, Fabiola Martín-Del-Campo, Enrique Rojas-Campos, Benjamín Gómez-Navarro
OBJECTIVE: Pica could be strongly implicated in nutritional status of patients on dialysis; however, very scarce data are currently available. The objective of this study was to evaluate the prevalence of pica and its association with nutritional status in dialysis patients. DESIGN AND METHODS: This is a cross-sectional study in a tertiary care teaching hospital. Four-hundred patients on dialysis, without previous pica diagnosis or transplant, pregnancy, mental illness, or infection, were included in the study...
October 12, 2018: Journal of Renal Nutrition
https://www.readbyqxmd.com/read/30321272/impact-of-a-poly-phenol-rich-extract-from-the-brown-algae-ascophyllum-nodosum-on-dna-damage-and-antioxidant-activity-in-an-overweight-or-obese-population-a-randomized-controlled-trial
#4
Francina R Baldrick, Kevin McFadden, Maria Ibars, Chris Sung, Tanya Moffatt, Kate Megarry, Keith Thomas, Peter Mitchell, Julie M W Wallace, L Kirsty Pourshahidi, Nigel G Ternan, Giulia Corona, Jeremy Spencer, Parveen Yaqoob, Sarah Hotchkiss, Ross Campbell, José Manuel Moreno-Rojas, Francisco Julián Cuevas, Gema Pereira-Caro, Ian Rowland, Chris I R Gill
Background: Epidemiologic evidence suggests that a diet rich in (poly)phenols has beneficial effects on many chronic diseases. Brown seaweed is a rich source of (poly)phenols. Objective: The aim of this study was to investigate the bioavailability and effect of a brown seaweed (Ascophyllum nodosum) (poly)phenol extract on DNA damage, oxidative stress, and inflammation in vivo. Design: A randomized, double-blind, placebo-controlled crossover trial was conducted in 80 participants aged 30-65 y with a body mass index (in kg/m2) ≥25...
October 1, 2018: American Journal of Clinical Nutrition
https://www.readbyqxmd.com/read/30317887/uptake-and-molecular-impact-of-aluminum-containing-nanomaterials-on-human-intestinal-caco-2-cells
#5
Holger Sieg, Caroline Braeuning, Birgitta Maria Kunz, Hannes Daher, Claudia Kästner, Benjamin-Christoph Krause, Thomas Meyer, Pégah Jalili, Kevin Hogeveen, Linda Böhmert, Dajana Lichtenstein, Agnès Burel, Soizic Chevance, Harald Jungnickel, Jutta Tentschert, Peter Laux, Albert Braeuning, Fabienne Gauffre, Valérie Fessard, Jan Meijer, Irina Estrela-Lopis, Andreas F Thünemann, Andreas Luch, Alfonso Lampen
Aluminum (Al) is one of the most common elements in the earth crust and increasingly used in food, consumer products and packaging. Its hazard potential for humans is still not completely understood. Besides the metallic form, Al also exists as mineral, including the insoluble oxide, and in soluble ionic forms. Representatives of these three species, namely a metallic and an oxidic species of Al-containing nanoparticles and soluble aluminum chloride, were applied to human intestinal cell lines as models for the intestinal barrier...
October 14, 2018: Nanotoxicology
https://www.readbyqxmd.com/read/30317444/profiling-of-nanoparticle-protein-interactions-by-electrophoresis-techniques
#6
REVIEW
Mohammad Zarei, Jamal Aalaie
The use of nanomaterials in the chemical, biomedical, and biotechnological sciences is growing, increasing the possible release of these materials into the environment and contact with living organisms. Because of their large surface area, biomolecules can be adsorbed on the surface of nanomaterials. Proteins bind to the surface of nanoparticles (NPs), forming a biological layer around the NP, the "protein corona," which gives new characteristics to the NPs in biological milieu and may affect biomedical applications or induce nanotoxicological effects...
October 13, 2018: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/30316298/polymer-coated-gold-ferric-oxide-superparamagnetic-nanoparticles-for-theranostic-applications
#7
Muhammad Raisul Abedin, Siddesh Umapathi, Harika Mahendrakar, Tunyaboon Laemthong, Holly Coleman, Denise Muchangi, Santimukul Santra, Manashi Nath, Sutapa Barua
BACKGROUND: Engineered inorganic nanoparticles (NPs) are essential components in the development of nanotechnologies. For applications in nanomedicine, particles need to be functionalized to ensure a good dispersibility in biological fluids. In many cases however, functionalization is not sufficient: the particles become either coated by a corona of serum proteins or precipitate out of the solvent. We show that by changing the coating of magnetic iron oxide NPs using poly-L-lysine (PLL) polymer the colloidal stability of the dispersion is improved in aqueous solutions including water, phosphate buffered saline (PBS), PBS with 10% fetal bovine serum (FBS) and cell culture medium, and the internalization of the NPs toward living mammalian cells is profoundly affected...
October 13, 2018: Journal of Nanobiotechnology
https://www.readbyqxmd.com/read/30308270/the-impact-of-protein-corona-on-the-behavior-and-targeting-capability-of-nanoparticle-based-delivery-system
#8
Wei Xiao, Huile Gao
Once introduced into physiological environment, nanoparticles (NPs) are immediately coated with proteins, resulting in formation of what is known as protein corona. The formation of protein corona can be affected by many factors. Likewise, the addition of protein corona can alter the physicochemical properties and biodistribution of NPs. NPs with the coating protein corona can be considered as a biological identity that recognized by cells in biological system. Thus, to understand and regulate the effect of protein corona on targeting capability of NPs in vivo, it is necessary to elucidate the interaction between the NPs and the biological fluid...
October 9, 2018: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/30298725/identification-of-myricetin-as-ebolavirus-vp35-dsrna-interaction-inhibitor-through-a-novel-fluorescence-based-assay
#9
Gian Luca Daino, Aldo Frau, Cinzia Sanna, Daniela Rigano, Simona Distinto, Veronica Madau, Francesca Esposito, Elisa Fanunza, Giulia Bianco, Orazio Taglialatela-Scafati, Luca Zinzula, Elias Maccioni, Angela Corona, Enzo Tramontano
The Filoviridae Ebola virus (EBOV) causes a severe and rapidly progressing hemorrhagic syndrome whose recent epidemic enlightened the urgent need of novel therapeutic agents, since no drug is currently approved. A key contribution to EBOV high lethality comes from viral evasion of the host antiviral innate immune response in which the viral protein VP35 plays a crucial role, blocking the interferon type I production, firstly by masking the viral dsRNA and preventing its detection by the pattern recognition receptor RIG-I...
October 9, 2018: Biochemistry
https://www.readbyqxmd.com/read/30274317/pegylation-of-superparamagnetic-iron-oxide-nanoparticles-with-self-organizing-polyacrylate-peg-brushes-for-contrast-enhancement-in-mri-diagnosis
#10
Erzsébet Illés, Márta Szekeres, Ildikó Y Tóth, Katalin Farkas, Imre Földesi, Ákos Szabó, Béla Iván, Etelka Tombácz
For biomedical applications, superparamagnetic nanoparticles (MNPs) have to be coated with a stealth layer that provides colloidal stability in biological media, long enough persistence and circulation times for reaching the expected medical aims, and anchor sites for further attachment of bioactive agents. One of such stealth molecules designed and synthesized by us, poly(polyethylene glycol methacrylate- co -acrylic acid) referred to as P(PEGMA-AA), was demonstrated to make MNPs reasonably resistant to cell internalization, and be an excellent candidate for magnetic hyperthermia treatments in addition to possessing the necessary colloidal stability under physiological conditions (Illés et al...
September 29, 2018: Nanomaterials
https://www.readbyqxmd.com/read/30248990/metallic-nanoparticles-general-research-approaches-to-immunological-characterization
#11
REVIEW
Francesca Gatto, Giuseppe Bardi
Our immunity is guaranteed by a complex system that includes specialized cells and active molecules working in a spatially and temporally coordinated manner. Interaction of nanomaterials with the immune system and their potential immunotoxicity are key aspects for an exhaustive biological characterization. Several assays can be used to unravel the immunological features of nanoparticles, each one giving information on specific pathways leading to immune activation or immune suppression. Size, shape, and surface chemistry determine the surrounding corona, mainly formed by soluble proteins, hence, the biological identity of nanoparticles released in cell culture conditions or in a living organism...
September 22, 2018: Nanomaterials
https://www.readbyqxmd.com/read/30243217/interference-of-engineered-nanomaterials-in-flow-cytometry-a-case-study
#12
Nils Bohmer, Alexandra Rippl, Sarah May, Aurélie Walter, Min Beom Heo, Minjeong Kwak, Matthias Roesslein, Nam Woong Song, Peter Wick, Cordula Hirsch
Nanotechnology is regarded as the enabling technology of the 21st century. However, only a relatively small number of nano-enabled medical and healthcare products finally made their way to the market. There are several reasons why such innovative approaches fail in translation, with one key factor being the uncertainty surrounding their safety assessment. Although well described, interference reactions of engineered nanomaterials (ENM) with classical cytotoxicity assays remain a major source of uncertainty...
September 12, 2018: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/30239120/size-shape-and-protein-corona-determine-cellular-uptake-and-removal-mechanisms-of-gold-nanoparticles
#13
Lin Ding, Chenjie Yao, Xiaofeng Yin, Chenchen Li, Yanan Huang, Min Wu, Bin Wang, Xiaoya Guo, Yanli Wang, Minghong Wu
Size, shape, and protein corona play a key role in cellular uptake and removal mechanisms of gold nanoparticles (Au NPs). The 15 nm nanoparticles (NP1), the 45 nm nanoparticles (NP2), and the rod-shaped nanoparticles (NR) enter into cells via a receptor-mediated endocytosis (RME) pathway. The star-shaped nanoparticles (NS) adopt not only clathrin-mediated, but also caveolin-mediated endocytosis pathways. However, the 80 nm nanoparitcles (NP3) mainly enter into the cells by macropinocytosis pathway due to the big size...
October 2018: Small
https://www.readbyqxmd.com/read/30229788/serum-protein-corona-responsive-autophagy-tuning-in-cells
#14
Huating Kong, Kai Xia, Ning Ren, Yunzhi Cui, Renduo Liu, Qingnuan Li, Min Lv, Jiye Shi, Qinglong Yan, Zhifen Cui, Chunhai Fan, Ying Zhu, Lihua Wang
Autophagy represents an important cellular response to nanoparticles (NPs), whose modulation holds great promise for developing nanomedicine. Here, we systematically studied cell autophagy responses elicited by the NP-protein corona with diverse protein corona types surrounding NPs with different sizes, shapes, and compositions. We demonstrated that these physicochemical properties of NP-protein coronas exerted a remarkable influence on cell autophagy responses. Particularly, for surface protein type-associated modulation of cell autophagy, we correlated the autophagy level to adsorbed protein type on Fe3O4 NPs...
September 27, 2018: Nanoscale
https://www.readbyqxmd.com/read/30225747/intercistronic-expression-elements-iee-from-the-chloroplast-of-chlamydomonas-reinhardtii-can-be-used-for-the-expression-of-foreign-genes-in-synthetic-operons
#15
Karla S Macedo-Osorio, Víctor H Pérez-España, Claudio Garibay-Orijel, Daniel Guzmán-Zapata, Noé V Durán-Figueroa, Jesús A Badillo-Corona
Two intercistronic regions were identified as functional intercistronic expression elements (IEE) for the simultaneous expression of aphA-6 and gfp in a synthetic operon in the chloroplast of C. reinhardtii. Chlamydomonas reinhardtii, a biflagellate photosynthetic microalga, has been widely used in basic and applied science. Already three decades ago, Chlamydomonas had its chloroplast genome transformed and to this day constitutes the only alga routinely used in transplastomic technology. Despite the fact that over a 100 foreign genes have been expressed from the chloroplast genome, little has been done to address the challenge of expressing multiple genes in the form of operons, a development that is needed and crucial to push forward metabolic engineering and synthetic biology in this organism...
September 17, 2018: Plant Molecular Biology
https://www.readbyqxmd.com/read/30222356/effects-of-protein-corona-on-active-and-passive-targeting-of-cyclic-rgd-peptide-functionalized-pegylation-nanoparticles
#16
Gaoxing Su, Huaqiao Jiang, Bohui Xu, Yanyan Yu, Xueqin Chen
Protein corona can alter the physiochemical properties of targeting nanoparticles (NPs), as well as their physiological responses and targeting functionality. Herein, we synthesized 20 types of NPs with diverse surface chemistry in order to study the impacts of protein corona on targeting functionality of NPs functionalized with cyclic RGD peptides and their relationships to the polyethylene glycol (PEG) length and grafting density of targeting ligands. After protein adsorption, cyclic RGD on the surface of NP was still able to bind its receptors with increased targeted cellular uptake, even at a relatively low density...
October 1, 2018: Molecular Pharmaceutics
https://www.readbyqxmd.com/read/30219059/impact-of-nanoparticle-surface-functionalization-on-the-protein-corona-and-cellular-adhesion-uptake-and-transport
#17
Ashraf Abdelkhaliq, Meike van der Zande, Ans Punt, Richard Helsdingen, Sjef Boeren, Jacques J M Vervoort, Ivonne M C M Rietjens, Hans Bouwmeester
BACKGROUND: Upon ingestion, nanoparticles can interact with the intestinal epithelial barrier potentially resulting in systemic uptake of nanoparticles. Nanoparticle properties have been described to influence the protein corona formation and subsequent cellular adhesion, uptake and transport. Here, we aimed to study the effects of nanoparticle size and surface chemistry on the protein corona formation and subsequent cellular adhesion, uptake and transport. Caco-2 intestinal cells, were exposed to negatively charged polystyrene nanoparticles (PSNPs) (50 and 200 nm), functionalized with sulfone or carboxyl groups, at nine nominal concentrations (15-250 μg/ml) for 10 up to 120 min...
September 15, 2018: Journal of Nanobiotechnology
https://www.readbyqxmd.com/read/30212621/how-entanglement-of-different-physicochemical-properties-complicates-the-prediction-of-in-vitro-and-in-vivo-interactions-of-gold-nanoparticles
#18
Ming Xu, Mahmoud G Soliman, Xing Sun, Beatriz Pelaz, Neus Feliu, Wolfgang J Parak, Sijin Liu
The physicochemical properties of a set of 21 different gold nanoparticles (spherical and rod-shaped nanoparticles (NPs) of different diameters with three different surface coatings) were studied. Protein corona formation, in vitro uptake, effect on cell viability and proliferation, and in vivo biodistribution of these NPs were determined. The relation of the results of the different NPs was analyzed by hierarchical cluster analysis, which will tell which NPs have the most similar physicochemical properties and biological effects, without having to specify individual physicochemical parameters...
September 21, 2018: ACS Nano
https://www.readbyqxmd.com/read/30209704/modeling-of-a-single-nanoparticle-interaction-with-the-human-blood-plasma-proteins
#19
Vladas Skakauskas, Pranas Katauskis
When nanoparticles are introduced into a physiological environment, proteins and lipids immediately cover their surface, forming a protein "corona". It is well recognized that the corona structure influences the biological response of the body. Two deterministic models for corona formation of the human blood serum proteins around a single nanoparticle are presented and studied numerically in this paper. One of them is based on a coupled system of PDEs and involves diffusion of proteins toward the nanoparticle surface...
September 12, 2018: Journal of Biological Physics
https://www.readbyqxmd.com/read/30198074/beyond-global-charge-role-of-amine-bulkiness-and-protein-fingerprint-on-nanoparticle-cell-interaction
#20
David Burnand, Ana Milosevic, Sandor Balog, Miguel Spuch-Calvar, Barbara Rothen-Rutishauser, Jörn Dengjel, Calum Kinnear, Thomas L Moore, Alke Petri-Fink
Amino groups presented on the surface of nanoparticles are well-known to be a predominant factor in the formation of the protein corona and subsequent cellular uptake. However, the molecular mechanism underpinning this relationship is poorly defined. This study investigates how amine type and density affect the protein corona and cellular association of gold nanoparticles with cells in vitro. Four specific poly(vinyl alcohol-co-N-vinylamine) copolymers are synthesized containing primary, secondary, or tertiary amines...
September 10, 2018: Small
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