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Protein Corona

Ibrahim Javed, Tianyu Yu, Guotao Peng, Antoni Sanchez-Ferrer, Ava Faridi, Aleksandr Kakinen, Mei Zhao, Raffaele Mezzenga, Thomas P Davis, Sijie Lin, Pu Chun Ke
Amyloid diseases are global epidemics with no cure available. Herein we report a first demonstration of in vivo mitigation of amyloidogenesis using a biomimetic nanotechnology. Specifically, the amyloid fragments (ba) of beta-lactoglobulin, a whey protein, were deposited onto the surfaces of carbon nanotubes (baCNT), which subsequently sequestered human islet amyloid polypeptide (IAPP) through functional-pathogenic double protein coronae. Conformational changes at the ba-IAPP interface were studied by Fourier transform infrared, circular dichroism, as well as X-ray scattering spectroscopies...
August 8, 2018: Nano Letters
Satoshi Furota, Nanako O Ogawa, Yoshinori Takano, Toshihiro Yoshimura, Naohiko Ohkouchi
The separation and quantification of underivatized naturally occurring amino acids (AAs) by high-performance liquid chromatography (HPLC) is advantageous for reducing preparation time, running costs, and analytical errors, and is compatible with the isolation of intact AAs. This study establishes and validates an analytical method for the separation and quantification of underivatized AAs and taurine (Tau) in the sub- to several-nanomolar range (ca. 0.1-1.6 nmol) by optimizing ion-pair HPLC coupled to a corona charged aerosol detector (corona CAD)...
July 25, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Fitya Syarifa Mozar, Ezharul Hoque Chowdhury
PEG-functionalized nanoparticles as carriers of chemotherapeutics agents have been explored with notable successes in preclinical and clinical stages of cancer treatment, with some already approved by FDA, namely PEGylated liposomes and polymers. Half-life extension of therapeutic agents through PEGylation process improves their pharmacokinetic (PK) profiles, thereby reducing their dosing frequency. Protein corona composition of PEGylated nanoparticles (NPs) confers tremendous influence on their surface characteristics which directly impact tumor accumulation and clearance properties of the drugs...
July 30, 2018: Current Pharmaceutical Design
Raphael Trösch, Rouhollah Barahimipour, Yang Gao, Jesús Agustín Badillo-Corona, Vincent Leon Gotsmann, David Zimmer, Timo Mühlhaus, Reimo Zoschke, Felix Willmund
Chloroplast gene expression is a fascinating and highly regulated process, which was mainly studied on specific genes in a few model organisms including the unicellular green alga Chlamydomonas (Chlamydomonas reinhardtii) and the embryophyte (land) plants tobacco (Nicotiana tabacum) and Arabidopsis (Arabidopsis thaliana). However, a direct plastid genome-wide interspecies comparison of chloroplast gene expression that includes translation was missing. We adapted a targeted chloroplast ribosome profiling approach to quantitatively compare RNA abundance and translation output between Chlamydomonas, tobacco and Arabidopsis...
July 30, 2018: Nature Plants
Juan Guan, Qing Shen, Zui Zhang, Zhuxuan Jiang, Yang Yang, Meiqing Lou, Jun Qian, Weiyue Lu, Changyou Zhan
Targeting ligands are anticipated to facilitate the precise delivery of therapeutic agents to diseased tissues; however, they may also severely affect the interaction of nanocarriers with plasma proteins. Here, we study the immunocompatibility of brain-targeted liposomes, which inversely correlates with absorbed natural IgM. Modification of long, stable positively charged peptide ligands on liposomes is inclined to absorb natural IgM, leading to rapid clearance and enhanced immunogenicity. Small peptidomimetic D8 developed by computer-aided peptide design exhibits improved immunocompatibility by attenuating natural IgM absorption...
July 30, 2018: Nature Communications
Marianna Barbalinardo, Federico Caicci, Massimiliano Cavallini, Denis Gentili
Medical applications of nanoparticles (NPs) require understanding of their interactions with living systems in order to control their physiological response, such as cellular uptake and cytotoxicity. When NPs are exposed to biological fluids, the adsorption of extracellular proteins on the surface of NPs, creating the so-called protein corona, can critically affect their interactions with cells. Here, the effect of surface coating of silver nanoparticles (AgNPs) on the adsorption of serum proteins (SPs) and its consequence on cellular uptake and cytotoxicity in mouse embryonic fibroblasts are shown...
July 29, 2018: Small
Fangyingkai Wang, Jingyi Gao, Jiangang Xiao, Jianzhong Du
An ideal gene carrier requires an excellent gating system to efficiently load, protect, deliver and release environmentally sensitive nucleic acids on demand. Presented in this communication is a polymersome with a "boarding gate" and a "debarkation gate" in the membrane to complete the above important missions. This dually gated polymersome is self-assembled from a block copolymer, poly(ethylene oxide)-block-poly[N-isopropyl acrylamide-stat-7-(2-methacryloyloxyethoxy)-4-methylcoumarin-stat-2-(diethylamino)ethyl methacrylate] [PEO-b-P(NIPAM-stat-CMA-stat-DEA)]...
July 27, 2018: Nano Letters
Dongdong Li, Junjie Wu, Xun Xu, Xingwei Wang, Shijia Yang, Ziqi Tang, Heng Shen, Xiaofang Liu, Ning Zhao, Jian Xu
Mussels exhibit robust adhesion capability with varied materials mainly due to the strong affinity of catechol moieties in their adhesive proteins. Nanoscale ionic materials (NIMs) are special organic-inorganic hybrid materials comprising a charged oligomer corona attached to inorganic nanoparticle cores, which can behave from glassy solids to liquids in the absence of any solvent. Herein, Fe3 O4 nanoscale ionic materials (NIMs) exhibiting inorganic-organic core-shell structure and liquid-like behavior were obtained by using a mussel-inspired bifunctional ligand of 3,4-dihydroxybenzenepropanoic acid (DHPA), which could link Fe3 O4 nanoparticles core and cationic organic shell, respectively...
July 23, 2018: Journal of Colloid and Interface Science
Maria Nicoletti, Claudia Capodanno, Cristian Gambarotti, Elisa Fasoli
BACKGROUND: the formation of bio-corona, due to adsorption of biomolecules onto carbon nanotubes (CNTs) surface in a physiological environment, may lead to a modified biological "identity" of CNTs, contributing to determination of their biocompatibility and toxicity. METHODS: multi-walled carbon nanotubes surfaces (f-MWCNTs) were modified attaching acid and basic chemical functions such as carboxyl (MWCNTs-COOH) and ammonium (MWCNTs-N) groups respectively...
July 23, 2018: Biochimica et Biophysica Acta
Atul Dhall, William Self
Cerium oxide nanoparticles (CeNPs) exhibit antioxidant properties both in vitro and in vivo. This is due to the self-regeneration of their surface, which is based on redox-cycling between 3+ and 4+ states for cerium, in response to their immediate environment. Additionally, oxygen vacancies in the lattice structure allow for alternating between CeO₂ and CeO2- x during redox reactions. Research to identify and characterize the biomedical applications of CeNPs has been heavily focused on investigating their use in treating diseases that are characterized by higher levels of reactive oxygen species (ROS)...
July 24, 2018: Antioxidants (Basel, Switzerland)
Iztok Urbančič, Maja Garvas, Boštjan Kokot, Hana Majaron, Polona Umek, Hilary Cassidy, Miha Škarabot, Falk Schneider, Silvia Galiani, Zoran Arsov, Tilen Koklic, David Matallanas, Miran Čeh, Igor Muševič, Christian Eggeling, Janez Štrancar
Although the link between the inhalation of nanoparticles and cardiovascular disease is well established, the causal pathway between nanoparticle exposure and increased activity of blood coagulation factors remains unexplained. To initiate coagulation tissue factor bearing epithelial cell membranes should be exposed to blood, on the other side of the less than a micrometre thin air-blood barrier. For the inhaled nanoparticles to promote coagulation, they need to bind lung epithelial-cell membrane parts and relocate them into the blood...
August 8, 2018: Nano Letters
Amanda N Abraham, Tarun K Sharma, Vipul Bansal, Ravi Shukla
The rapid formation of the protein corona on to the nanoparticle (NP) surface is the key that confers biological identity to NPs and subsequently dictates their fate both in vitro and in vivo. Despite significant efforts, the inability to control the spontaneous interaction of serum proteins with the administered NPs remains a major constraint in clinical translation of nanomedicines. The ligands present on the NP surface offer promise in controlling their biological interactions; however, their influence on the NP-protein interaction is not well-understood...
February 28, 2018: ACS Omega
Dong Zhang, Jincheng Yang, Jibin Guan, Bin Yang, Shenwu Zhang, Mengchi Sun, Ruitao Yang, Tao Zhang, Ruoshi Zhang, Qiming Kan, Haotian Zhang, Zhonggui He, Lei Shang, Jin Sun
Prodrug self-nanoassemblies have many advantages for anticancer drug delivery, including high drug loading rate, resistance to recrystallization, and on-demand drug release. However, few studies have focused on their protein corona, which is inevitably formed after entering the blood and determines their subsequent fates in vivo. To actively tune the protein corona of prodrug nanoassemblies, three maleimide-paclitaxel prodrugs were synthesized via different redox-sensitive linkers (ester bond, thioether bond and disulfide bond)...
July 18, 2018: Biomaterials Science
Naihao Lu, Yinhua Sui, Lizeng Zeng, Rong Tian, Yi-Yuan Peng
The interactions between proteins and bioactive ligands (such as flavonoids and nanomaterials) are vital to the design of effective protein carriers for the protection of bioactive molecules and reduction of the cytotoxicity of nanotubes. Bovine serum albumin (BSA) can bind various bioactive components and subsequently form protein-ligand complexes. Herein, the binding of BSA to quercetin and single-walled carbon nanotubes (SWCNTs) was investigated by using experimental and molecular-docking methods. The fluorescence intensity of BSA was decreased by both quercetin and SWCNTs in static quenching mode (i...
August 8, 2018: Journal of Agricultural and Food Chemistry
Antonietta Arcella, Sara Palchetti, Luca Digiacomo, Daniela Pozzi, Anna Laura Capriotti, Luigi Frati, Maria Antonietta Oliva, Georgia Tsaouli, Rossella Rota, Isabella Screpanti, Morteza Mahmoudi, Giulio Caracciolo
Temozolomide (TMZ) is the current first-line chemotherapy for treatment of glioblastoma multiforme (GBM). However, similar to other brain therapeutic compounds, access of TMZ to brain tumors is impaired by the blood-brain barrier (BBB) leading to poor response for GBM patients. To overcome this major hurdle, we have synthesized a set of TMZ-encapsulating nanomedicines made of four cationic liposome (CL) formulations with systematic changes in lipid composition and physical-chemical properties. The targeting nature of this nanomedicine is provided by the recruitment of proteins, with natural targeting capacity, in the biomolecular corona (BC) layer that forms around CLs after exposure to human plasma (HP)...
July 31, 2018: ACS Chemical Neuroscience
Luz E Casados-Vázquez, Dennis K Bideshi, José E Barboza-Corona
The structural gene that encodes thurincin H, a bacteriocin produced by Bacillus thuringiensis, is harboured in a genetic cluster (thnP, E, D, R, A1, A2, A3, B, T, I) that controls its synthesis, modification, secretion and autoimmunity. The specific genes in the cassette that confer immunity in B. thuringiensis to thurincin H are unknown. To identify these immunity determinants, we generated constructs that were used to transform a natural thurincin H-sensitive B. thuringiensis strain (i.e. Btk 404), and resistance or susceptibility to the bacteriocin in resultant recombinants was evaluated...
July 13, 2018: Antonie Van Leeuwenhoek
Tatiana Borisova
Nerve cells take a special place among other cells in organisms because of their unique function mechanism. The plasma membrane of nerve cells from the one hand performs a classical barrier function, thereby being foremost targeted during contact with micro- and nano-sized particles, and from the other hand it is very intensively involved in nerve signal transmission, i.e., depolarization-induced calcium-dependent compound exocytosis realized via vesicle fusion following by their retrieval and calcium-independent permanent neurotransmitter turnover via plasma membrane neurotransmitter transporters that utilize Na+ /K+ electrochemical gradient as a driving force...
2018: Frontiers in Physiology
Dzmitry Shcharbin, Elzbieta Pedziwiatr-Werbicka, Tatyana Serchenya, Sylwia Cyboran-Mikolajczyk, Lena Prakhira, Viktar Abashkin, Volha Dzmitruk, Maksim Ionov, Svetlana Loznikova, Iryna Shyrochyna, Oleg Sviridov, Cornelia E Peña-González, Andrea Barrios Gumiel, Rafael Gómez, F Javier de la Mata, Maria Bryszewska
Functionalization of gold nanoparticles by different chemical groups is an important issue regarding the biomedical applications of such particles. Therefore we have analyzed the interaction between gold nanoparticles functionalized by carbosilane dendrons with human serum albumin at different pHs, and in the presence of the protein unfolding agent, guanidine hydrochloride, using circular dichroism, zeta-potential and fluorescence quenching. The effect of a nanoparticle dendronization and pure dendrons on the immunoreactivity of albumin was estimated using ELISA...
July 8, 2018: International Journal of Biological Macromolecules
Luca Guerrini, Ramon A Alvarez-Puebla, Nicolas Pazos-Perez
Due to the high surface: volume ratio and the extraordinary properties arising from the nanoscale (optical, electric, magnetic, etc.), nanoparticles (NPs) are excellent candidates for multiple applications. In this context, nanoscience is opening a wide range of modern technologies in biological and biomedical fields, among others. However, one of the main drawbacks that still delays its fast evolution and effectiveness is related to the behavior of nanomaterials in the presence of biological fluids. Unfortunately, biological fluids are characterized by high ionic strengths which usually induce NP aggregation...
July 6, 2018: Materials
Chang Tian, Jie Feng, H Jeremy Cho, Sujit S Datta, Robert K Prud'homme
Nanoparticles (NPs) have been widely applied in fields as diverse as energy conversion, photovoltaics, environment remediation, and human health. However, the adsorption and trapping of NPs interfaces is still poorly understood, and few studies have characterized the kinetics quantitatively. In many applications, such as drug delivery, understanding NP interactions at an interface is essential to determine and control adsorption onto targeted areas. Therapeutic NPs are especially interesting because their structures involve somewhat hydrophilic surface coronas, to prevent protein adsorption, and much more hydrophobic core phases...
July 12, 2018: Nano Letters
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