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Protein Corona

Xitong Liu, Chenxu Yan, Kai Loon Chen
The influence of solution chemistry on the adsorption of human serum albumin (HSA) proteins on graphene oxide (GO) was investigated through batch adsorption experiments and the use of a quartz crystal microbalance with dissipation (QCM-D). The conformation of HSA layers on GO was also examined with the QCM-D. Our results show that an increase in ionic strength under neutral pH conditions resulted in stronger binding between HSA and GO, as well as more compact HSA layers on GO, emphasizing the key role of electrostatic interactions in controlling HSA-GO interactions...
December 4, 2018: Environmental Science & Technology
Debolina Chakraborty, Lavanya Mohan, Sruthi Ann Alex, Natarajan Chandrasekaran, Amitava Mukherjee
The biomedical applicability of gold nanorods (AuNRs) arises due to their interesting optical and photothermal properties, which can result in the formation of a protein corona layer when exposed to the physiological system. The current study focuses on the effect of bimetallic coatings of AuNRs (AuNRs@Pd and AuNRs@Cu) on protein corona formation, and the potential application of protein-coronated bimetallic AuNRs was investigated for doxorubicin (dox) loading, release, and in vitro cytotoxicity analysis. Two significant proteins in blood serum, namely, human serum albumin (HSA) and transferrin, were chosen for the protein coronation...
December 4, 2018: Biomaterials Science
Marilena Hadjidemetriou, Sarah McAdam, Grace Garner, Chelsey Thackeray, David Knight, Duncan Smith, Zahraa Al-Ahmady, Mariarosa Mazza, Jane Rogan, Andrew Clamp, Kostas Kostarelos
The self-assembled layered adsorption of proteins onto nanoparticle (NP) surfaces, once in contact with biological fluids, is termed the "protein corona" and it is gradually seen as a determinant factor for the overall biological behavior of NPs. Here, the previously unreported in vivo protein corona formed in human systemic circulation is described. The human-derived protein corona formed onto PEGylated doxorubicin-encapsulated liposomes (Caelyx) is thoroughly characterized following the recovery of liposomes from the blood circulation of ovarian carcinoma patients...
November 28, 2018: Advanced Materials
Aohua Wang, Tiantian Yang, Weiwei Fan, Yiwei Yang, Quanlei Zhu, Shiyan Guo, Chunliu Zhu, Yongchun Yuan, Tao Zhang, Yong Gan
Oral delivery of peptide/protein drugs has attracted worldwide attention due to its good patient compliance and convenience of administration. Orally administered nanocarriers always encounter the rigorous defenses of the gastrointestinal tract, which mainly consist of mucus and epithelium barriers. However, diametrically opposite surface properties of nanocarriers are required for good mucus penetration and high epithelial uptake. Here, bovine serum albumin (BSA) is adsorbed to cationic liposomes (CLs) to form protein corona liposomes (PcCLs)...
November 28, 2018: Advanced Healthcare Materials
Regina Holm, Marcel Douverne, Benjamin Weber, Tobias Bauer, Andreas Best, Patrick Ahlers, Kaloian Koynov, Pol Besenius, Matthias Barz
The size control of nanomedicines for tumor diagnosis and therapy is of high importance, since it enables or disables deep and sufficient tumor penetration. Amphiphilic star shaped block copolypept(o)ides offer substantial promise to precisely adjust the hydrophobic core and the hydrophilic corona, independent of each other, and therefore, simultaneously control the size dimension in the interesting size range from 10 - 30 nm. To gain access to core-shell structures of such sizes, 3-arm and 6-arm PeptoStars, based on poly(γ-t-butyloxycarbonyl-L-glutamate)-b-polysarcosine (pGlu(OtBu)-b-pSar), were prepared via controlled living ring-opening polymerization (ROP) of the corresponding N-carboxyanhydrides...
November 26, 2018: Biomacromolecules
G Cozza, M Fortuna, F Meggio, S Sarno, M H G Kubbutat, F Totzke, C Schaechtele, L A Pinna, E N Olsufyeva, M N Preobrazhenskaya
As key regulators of cell signaling, protein kinases (PKs) are attractive targets for therapeutic intervention in a variety of diseases. Herein, we report for the first time the inhibitory activity of polycyclic peptides, particularly, derivatives of glycopeptide antibiotics teicoplanin and eremomycin, against a panel of 12 recombinant human protein kinases and two protein kinases (CK1 and CK2) isolated from rat liver. Several of the investigated compounds inhibited various PKs with IC50 values below 10 μM and caused >90% suppression of the enzyme activity at 10 µM concentration...
October 2018: Biochemistry. Biokhimii︠a︡
Paola Crociara, Maria Novella Chieppa, Elena Vallino Costassa, Elena Berrone, Marina Gallo, Monica Lo Faro, Maria Domenica Pintore, Barbara Iulini, Antonio D'Angelo, Giovanni Perona, Alberto Botter, Donato Formicola, Alberto Rainoldi, Marianna Paulis, Paolo Vezzoni, Federica Meli, Fiorenzo A Peverali, Caterina Bendotti, Maria Chiara Trolese, Laura Pasetto, Valentina Bonetto, Giovanna Lazzari, Roberto Duchi, Andrea Perota, Irina Lagutina, Corinne Quadalti, Maria Silvia Gennero, Daniela Dezzutto, Rosanna Desiato, Marina Boido, Matilde Ghibaudi, Maria Consuelo Valentini, Maria Caramelli, Cesare Galli, Cristina Casalone, Cristiano Corona
Amyotrophic Lateral Sclerosis (ALS) is a neural disorder gradually leading to paralysis of the whole body. Alterations in superoxide dismutase SOD1 gene have been linked with several variants of familial ALS. Here, we investigated a transgenic (Tg) cloned swine model expressing the human pathological hSOD1G93A allele. As in patients, these Tg pigs transmitted the disease to the progeny with an autosomal dominant trait and showed ALS onset from about 27 months of age. Post mortem analysis revealed motor neuron (MN) degeneration, gliosis and hSOD1 protein aggregates in brainstem and spinal cord...
November 21, 2018: Neurobiology of Disease
Karen S González-Ponce, Luz E Casados-Vázquez, Paulina Lozano-Sotomayor, Dennis K Bideshi, Ma Cristina Del Rincón-Castro, José E Barboza-Corona
Our objective was to determine whether a recombinant chitinase ChiA74∆sp of Bacillus thuringiensis and its truncated versions (ChiA74∆sp-60, ChiA74∆sp-50) could be produced in B. thuringiensis HD1 with no detrimental effect on the size and insecticidal activity of the native bipyramidal Cry crystal. chiA-p, the promoter used to drive chitinase gene expression, was active during vegetative growth of Cry- B. HD1 recombinants showed increases from ~7- to 12-fold in chitinase activity when compared with parental HD1 and negligible or no effect on the volume of bipyramidal crystals was observed...
November 22, 2018: International Journal of Biological Macromolecules
Elisa Fanunza, Aldo Frau, Angela Corona, Enzo Tramontano
Upon viral infection, the interferon (IFN) system triggers potent antiviral mechanisms limiting viral growth and spread. Hence, to sustain their infection, viruses evolved efficient counteracting strategies to evade IFN control. Ebola virus (EBOV), member of the family Filoviridae, is one of the most virulent and deadly pathogen ever faced by humans. Etiological agent of the Ebola virus disease (EVD), EBOV can be undoubtedly considered the perfect example of a powerful inhibitor of the host organism immune response activation...
November 23, 2018: Infectious Disorders Drug Targets
Sheyda Shakiba, Alireza Hakimian, Luis R Barco, Stacey Marie Louie
Engineered nanoparticles (NPs) will obtain macromolecular coatings in environmental systems, changing their subsequent interactions. The matrix complexity inherent in natural waters and wastewaters greatly complicates prediction of the corona formation. Here, we investigate corona formation on titanium dioxide (TiO2 ) NPs from mixtures of natural organic matter (NOM) and a protein, bovine serum albumin (BSA), to thoroughly probe the role of mixture interactions in the adsorption process. Fundamentally different coronas were observed under different NP exposure conditions and time scales...
November 21, 2018: Environmental Science & Technology
Mahtab Asadian, Maarten Dhaenens, Iuliia Onyshchenko, Stijn De Waele, Heidi Declercq, Pieter Cools, Bart Devreese, Dieter Deforce, Rino Morent, Nathalie De Geyter
The surface properties of electrospun scaffolds can greatly influence protein adsorption and thus strongly dictate cell-material interactions. In this study, we aim to investigate possible correlations between the surface properties of argon, nitrogen and ammonia/helium plasma-functionalized polycaprolactone (PCL) nanofibers (NFs) and their cellular interactions by examining the protein corona patterns of the plasma-treated NFs as well as the cell membrane proteins involved in cell proliferation. As a result of the performed plasma treatments, PCL NFs morphology was preserved while wettability was improved profoundly after all treatments because of the incorporation of polar surface groups...
November 16, 2018: ACS Applied Materials & Interfaces
Fernando Corona, Jose Antonio Reales-Calderón, Concha Gil, José Luis Martínez
Bacterial physiology is regulated at different levels, from mRNA synthesis to translational regulation and protein modification. Herein, we propose a parameter, dubbed post-transcriptional variation (PTV), that allows extracting information on post-transcriptional regulation from the combined analysis of transcriptomic and proteomic data. We have applied this parameter for getting a deeper insight in the regulon of the Pseudomonas aeruginosa post-transcriptional regulator Crc. P. aeruginosa is a free-living microorganism, and part of its ecological success relies on its capability of using a large number of carbon sources...
November 14, 2018: Scientific Reports
Johanna Simon, Julius Müller, Artur Ghazaryan, Svenja Morsbach, Volker Mailänder, Katharina Landfester
Adsorption of blood proteins to the surface of nanocarriers is known to be the critical factor influencing cellular interactions and eventually determining the successful application of nanocarriers as drug carriers in vivo. There is an increasing number of reports summarizing large data sets of all identified corona proteins. However, to date our knowledge about the multiple mechanisms mediating interactions between proteins and nanocarriers is still limited. In this study, we investigate the influence of protein structure on the adsorption process and focus on the effect of heat inactivation of serum and plasma, which is a common cell culture procedure used to inactivate the complement system...
November 14, 2018: Nanoscale
Xi Zhang, Arun Kumar Pandiakumar, Robert J Hamers, Catherine J Murphy
Formation of a protein corona around nanoparticles when immersed into biological fluids is well-known; less studied is the formation of lipid coronas around nanoparticles. In many cases, the identity of a nanoparticle-acquired corona determines nanoparticle fate within a biological system and its interactions with cells and organisms. This work systematically explores the impact of nanoparticle surface chemistry and lipid character on the formation of lipid coronas for 3 different nanoparticle surface chemistries (2 cationic, 1 anionic) on 14 nm gold nanoparticles exposed to a series of lipid vesicles of 4 different compositions...
November 20, 2018: Analytical Chemistry
Jian Zhao, Shengming Wu, Jingwen Qin, Donglu Shi, Yilong Wang
A critical issue in nanomedicine is on the understanding of nano-bio interface behaviors, particularly when the nanoparticles are inevitably decorated by protein coronas in the physiological fluids. In this study, the effects of particle surface corona on cancer cell targeting were investigated in simulated physiological fluids. Cell targeting was achieved by two strategies: (1) using conventional EpCAM antibody-functionalized Fe3O4 nanoparticles, and (2) rendering the same but naked magnetic nanoparticles electrically-positively charged enabling them to electrostatically bind onto the negatively-charged cancer cells...
November 14, 2018: ACS Applied Materials & Interfaces
Jessica Fernanda Affonso de Oliveira, Francine Scheffer, Ryan F Landis, Erico Teixeira-Neto, Vincent M Rotello, Mateus Borba Cardoso
Protein coronas form on the surfaces of nanomaterials in biological fluids. This layer of proteins affects the properties of nanomaterials, altering their behavior and masking engineered functionality. The use of non-fouling moieties reduces or prevents corona formation, however these ligands typically complicate functionalization. We present here a surface modification strategy for silica nanoparticles using specific molar ratios of zwitterionic and amine moieties. Through proper balance of ligands, we were able to generate particles that featured reactive 'handles', while retaining non-fouling properties, high hemocompatibility and low cytotoxicity...
November 14, 2018: ACS Applied Materials & Interfaces
Fernando Corona, José Luis Martínez, Pablo I Nikel
The remarkable metabolic versatility of bacteria of the genus Pseudomonas enable their survival across very diverse environmental conditions. P. aeruginosa, one of the most relevant opportunistic pathogens, is a prime example of this adaptability. The interplay between regulatory networks that mediate these metabolic and physiological features is just starting to be explored in detail. Carbon catabolite repression, governed by the Crc protein, controls the availability of several enzymes and transporters involved in the assimilation of secondary carbon sources...
November 8, 2018: Environmental Microbiology
Yawo A Mondjinou, Bradley P Loren, Christopher J Collins, Seok-Hee Hyun, Asher Demoret, Joseph Skulsky, Cheyenne Chaplain, Vivek Badwaik, David H Thompson
A family of five water-soluble Gd3+ :1,4,7,10-tetraazacyclododecane-1,4,7-tetraacetic acid-modified polyrotaxane (PR) magnetic resonance contrast agents bearing mixtures of 2-hydroxypropyl-β-cyclodextrin and 4-sulfobutylether-β-cyclodextrin macrocycles threaded onto Pluronic cores were developed as long circulating magnetic resonance contrast agents. Short diethylene glycol diamine spacers were utilized for linking the macrocyclic chelator to the PR scaffold prior to Gd3+ chelation. The PR products were characterized by 1 H NMR, gel permeation chromatography/multiangle light scattering, dynamic light scattering, and analytical ultracentrifugation...
November 7, 2018: Bioconjugate Chemistry
Bradley B Stocks, Jeremy E Melanson
Disulfide bonds are critical linkages for maintaining protein structure and enzyme activity. These linkages, however, can limit peptide sequencing efforts by mass spectrometry (MS) and often require chemical reduction and alkylation. Under such conditions, information regarding cysteine connectivity is lost. Online partial disulfide reduction within the electrospray (ESI) source has recently been established as a means to identify complex cysteine linkage patterns in a liquid chromatography-MS experiment without the need for sample pre-treatment...
November 6, 2018: Analytical and Bioanalytical Chemistry
Jun Yong Oh, Han Sol Kim, L Palanikumar, Eun Min Go, Batakrishna Jana, Soo Ah Park, Ho Young Kim, Kibeom Kim, Jeong Kon Seo, Sang Kyu Kwak, Chaekyu Kim, Sebyung Kang, Ja-Hyoung Ryu
Targeted drug delivery using nanoparticles can minimize the side effects of conventional pharmaceutical agents and enhance their efficacy. However, translating nanoparticle-based agents into clinical applications still remains a challenge due to the difficulty in regulating interactions on the interfaces between nanoparticles and biological systems. Here, we present a targeting strategy for nanoparticles incorporated with a supramolecularly pre-coated recombinant fusion protein in which HER2-binding affibody combines with glutathione-S-transferase...
October 31, 2018: Nature Communications
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