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Ph monitoring

Cheng Chen, Yang Xiang, Ya Liu, Xiangdong Hu, Ke-Wu Yang
The 'superbug' infection caused by metallo-β-lactamases (MβLs) including L1 has grown into an emerging threat. To probe whether mercaptoacetate thioesters inhibiting L1 is a contribution of the thioester itself or its hydrolysate, ten mercaptoacetate thioesters 1-10 were synthesized, which specifically inhibited L1, exhibiting IC50 values ranging from 0.17 to 1.2 μM, and 8 was found to be the best inhibitor (IC50 = 0.17 μM). These thioesters restored the antimicrobial activity of cefazolin against E. coli expressing L1 by 2-4-fold...
July 1, 2018: MedChemComm
Lucienne Nouchikian, Cristopher Roque, Jimmy Y Song, Nausheen Rahman, Salvador F Ausar
Determination of protein concentration in vaccines containing aluminum salt adjuvant typically necessitates desorption of the protein prior to analysis. Here we describe a method based on the intrinsic fluorescence of tyrosine and tryptophan that requires no desorption of proteins. Adjuvanted formulations of three model Bordetella pertussis antigens were excited at 280 nm and their emission spectra collected from 290 to 400 nm. Emission spectra of protein antigens in the presence of aluminum salt adjuvants were able to be detected, the effects of adjuvants on the spectra were analyzed, and linear regressions were calculated...
August 11, 2018: Vaccine
Teuta Pilizota, Ya-Tang Yang
With the emergence of inexpensive 3D printing technology, open-source platforms for electronic prototyping and single-board computers, "Do it Yourself" (DIY) approaches to the cultivation of microbial cultures are becoming more feasible, user-friendly, and thus wider spread. In this perspective, we survey some of these approaches, as well as add-on solutions to commercial instruments for synthetic and system biology applications. We discuss different cultivation designs, including capabilities and limitations...
2018: Frontiers in Microbiology
Victoire M C Rérolle, Eric P Achterberg, Mariana Ribas-Ribas, Vassilis Kitidis, Ian Brown, Dorothee C E Bakker, Gareth A Lee, Matthew C Mowlem
Increasing atmospheric CO₂ concentrations are resulting in a reduction in seawater pH, with potential detrimental consequences for marine organisms. Improved efforts are required to monitor the anthropogenically driven pH decrease in the context of natural pH variations. We present here a high resolution surface water pH data set obtained in summer 2011 in North West European Shelf Seas. The aim of our paper is to demonstrate the successful deployment of the pH sensor, and discuss the carbonate chemistry dynamics of surface waters of Northwest European Shelf Seas using pH and ancillary data...
August 10, 2018: Sensors
Daniel Feliciano, Jonathon Nixon-Abell, Jennifer Lippincott-Schwartz
Different multicellular organisms undergo cell-cell fusion to form functional syncytia that support specialized functions necessary for proper development and survival. For years, monitoring the structural consequences of this process using live-cell imaging has been challenging due to the unpredictable timing of cell fusion events in tissue systems. Here we present a triggered vesicular stomatitis virus G-protein (VSV-G)-mediated cell-cell fusion assay that can be used to synchronize fusion between cells. This allows the study of cellular changes that occur during cell fusion...
August 13, 2018: Current Protocols in Cell Biology
Yukihiro Esaka, Kenji Hisato, Takuhei Yamamoto, Hiroya Murakami, Bunji Uno
The substrate selectivities of three endonucleases were studied quantitatively using capillary zone electrophoresis to find one giving N2 -ethyl(Et)-2'-deoxyguanosine-5'-monophosphate (5'-dGMP) and cyclic 1,N2 -propano(CPr)-5'-dGMP from DNAs damaged by acetaldehyde (AA). Six 2'-deoxyribonucleoside-5'-monophosphates to be quantified in the hydrolysis solutions of DNAs, namely, Et-5'-dGMP, CPr-5'-dGMP, and four authentic ones, were completely separated using a 100 mM borate running buffer solution having an optimized pH of 9...
2018: Analytical Sciences: the International Journal of the Japan Society for Analytical Chemistry
Long T Ho, Duy T Pham, Wout Van Echelpoel, Andres Alvarado, Juan E Espinoza-Palacios, Maria B Arevalo-Durazno, Peter L M Goethals
Algal photosynthesis plays a key role in the removal mechanisms of waste stabilization ponds (WSPs), which is indicated in the variations of three parameters, dissolved oxygen, pH, and chlorophyll a. These variations can be considerably affected by extreme climatic conditions at high altitude. To investigate these effects, three sampling campaigns were conducted in a high-altitude WSP in Cuenca (Ecuador). From the collected data, the first application of structure equation modeling (SEM) on a pond system was fitted to analyze the influence of high-altitude characteristics on pond performance, especially on the three indicators...
August 2018: Water Science and Technology: a Journal of the International Association on Water Pollution Research
Yifan Zhou, Luis Alejandro Mur, Arwyn Edwards, John Davies, Jiwan Han, Huawei Qin, Ying Ye
Freshwater quality detection is important for pollution control. Three important components of water quality are pH, ammonia and dissolved H2 S and there is an urgent need for a high-precision sensor for simultaneous and continuous measurement. In this study, all-solid-state electrodes of Eh, pH, NH4 + and S2- were manufactured and mounted to a wireless chemical sensor with multiple parameters. Calibration indicated that the pH electrode had a Nernst response with slope of 53.174 mV; the NH4 + electrode had a detection limit of 10-5 mol/L (Nernst response slope of 53...
August 2018: Water Science and Technology: a Journal of the International Association on Water Pollution Research
Zaher Fanari, Asim A Mohammed, Jaya D Bathina, Desiree T Hodges, Kelsey Doorey, Nicholas Gagliano, Kirk N Garratt, William S Weintraub, Andrew J Doorey
BACKGROUND: Benzodiazepines and opioids are commonly used for conscious sedation (CS) in cardiac catheterization laboratory (CCL) patients. Both drugs are known to predispose to hypoxemia, apnea and decreased responsiveness to PCO2 , resulting in decreased arterial pH and PO2 , as well as increased PCO2 . We want to determine the effects of CS on arterial blood gas (ABG) in CCL patient, and identify if pulse oximetry monitoring is adequate. METHODS: We enrolled 18 subjects undergoing elective catheterization...
August 1, 2018: Cardiovascular Revascularization Medicine: Including Molecular Interventions
Ganesh Raghu, Carlos A Pellegrini, Eric Yow, Kevin R Flaherty, Keith Meyer, Imre Noth, Mary Beth Scholand, John Cello, Lawrence A Ho, Sudhakar Pipavath, Joyce S Lee, Jules Lin, James Maloney, Fernando J Martinez, Ellen Morrow, Marco G Patti, Stan Rogers, Paul J Wolters, Robert Yates, Kevin J Anstrom, Harold R Collard
BACKGROUND: Abnormal acid gastro-oesophageal reflux (GER) is hypothesised to play a role in progression of idiopathic pulmonary fibrosis (IPF). We aimed to determine whether treatment of abnormal acid GER with laparoscopic anti-reflux surgery reduces the rate of disease progression. METHODS: The WRAP-IPF trial was a randomised controlled trial of laparoscopic anti-reflux surgery in patients with IPF and abnormal acid GER recruited from six academic centres in the USA...
August 9, 2018: Lancet Respiratory Medicine
Filip Vasilev, Nunzia Limatola, Dae-Ryoung Park, Uh-Hyun Kim, Luigia Santella, Jong Tai Chun
BACKGROUND/AIMS: Eggs of all animal species display intense cytoplasmic Ca2+ increases at fertilization. Previously, we reported that unfertilized eggs of Astropecten aranciacus exposed to an actin drug latrunculin A (LAT-A) exhibit similar Ca2+ waves and cortical flashes after 5-10 min time lag. Here, we have explored the molecular mechanisms underlying this unique phenomenon. METHODS: Starfish eggs were pretreated with various agents such as other actin drugs or inhibitors of phospholipase C (PLC), and the changes of the intracellular Ca2+ levels were monitored by use of Calcium Green in the presence or absence of LAT-A...
August 10, 2018: Cellular Physiology and Biochemistry
Fangfang Qu, Dong Ren, Yong He, Pengcheng Nie, Lei Lin, Chengyong Cai, Tao Dong
In this work, the near infrared spectroscopy (NIR) technology was applied to nondestructively evaluate the freshness of pork. The total volatile basic-nitrogen (TVB-N) and pH value of pork were detected as freshness evaluation indicators. A multi-index statistical information fusion (MSIF) modeling method based on variable selection was proposed to evaluate pork freshness. In the experiment, the proposed MSIF was compared with other state-of-the-art variable selection methods. Results showed that the proposed method achieved the best generalization performance and stability...
July 25, 2018: Meat Science
Adriano Evandir Marchello, Natalia Lombardi Oliveira, Ana Teresa Lombardi, Adriano Polpo
Aquatic ecosystems are composed by a myriad of dissolved organic materials that can be assimilated by microalgae, while they can perform photosynthesis, this is refereed as mixotrophy. However, ecotoxicological tests usually consider only the photoautotrophic metabolism. This research investigated the ecotoxicological differences between photoautotrophy and mixotrophy in Chlorella sorokiniana exposed to cadmium (Cd). Chlorophyll a, photosynthetic efficiency (Fv/Fm), cell viability, biochemical composition and pH were used to monitor possible toxic effects at 72 h cultures...
August 6, 2018: Chemosphere
Lucy Haque, Sutanwi Bhuiya, Indrajit Giri, Susmita Chowdhury, Suman Das
Polymorphism of DNA plays a very important part of research relating to the drug-DNA interactions. Here main focus of our investigation is to monitor the interaction of the benzophenanthridine plant alkaloid, nitidine (NIT) with two different forms of DNA i.e. B-DNA and protonated form of DNA maintaining proper temperatures and buffer conditions. Binding interaction of NIT was ascertained from the UV-Visible spectroscopic and spectrofluorimetric titration experiments. Binding constants of the interactions of NIT with different polymorphic forms were calculated from UV-absorption study...
August 8, 2018: International Journal of Biological Macromolecules
Mara Reifenrath, Eckhard Boles
Many cellular processes are regulated via pH, and maintaining the pH of different organelles is crucial for cell survival. A pH-sensitive GFP variant, the so-called pHluorin, has proven to be a valuable tool to study the pH of the cytosol, mitochondria and other organelles in vivo. We found that the fluorescence intensity of Endoplasmic Reticulum (ER)-targeted pHluorin in the yeast Saccharomyces cerevisiae was very low and barely showed pH sensitivity, probably due to misfolding in the oxidative environment of the ER...
August 10, 2018: Scientific Reports
F M M Meijer, P Kies, M R M Jongbloed, S E van Wijngaarden, C A Swenne, S Man, M J Schalij, J K de Vries-Bouwstra, H W Vliegen
BACKGROUND: Patients with systemic sclerosis (SSc) are at risk for developing pulmonary hypertension (PH) which is a major cause of death in this population. Echocardiographic (TTE) derived pulmonary arterial pressure (PAP) can be unreliable for the early detection of PH. Previous studies demonstrate that the ECG derived ventricular gradient optimized for right ventricular pressure overload (VG-RVPO) can detect PH in a heterogeneous population suspected of PH. The aim of this study is to assess the use of the VG-RVPO as a screening and monitoring instrument of early PH in SSc patients...
July 24, 2018: International Journal of Cardiology
Amal A El-Masry, Mohammed E A Hammouda, Dalia R El-Wasseef, Saadia M El-Ashry
Background: Green solvents such as microemulsion were used in the proposed method because they play a vital role in the analytical method's influence on the environment. Objective: A highly sensitive, specific, and validated stability-indicating eco-friendly green microemulsion liquid chromatography (MELC) method was developed for separation of the antihistaminic drug Azelastine HCl (AZL) from its degradation products with application to degradation kinetics. Methods: Chromatographic separation was operated on a C18 column with a microemulsion mobile phase, which consists of 0...
August 10, 2018: Journal of AOAC International
Tal Weissbach, Ishai Heusler, Michal Ovadia, Liron David, Yair Daykan, Faye Schreiber, Tal Biron-Shental
OBJECTIVE: To correlate the duration of Category II cardiotocograms (CTG) with adverse neonatal outcomes associated with perinatal asphyxia and determine the duration before fetal compromise. STUDY DESIGN: This retrospective, observational study used electronic medical record data from a cohort of 271 patients, delivered by C-section due to non-reassuring fetal heart rate, at a tertiary medical center, from 2015 through 2017. Duration of Category II CTG, variability, tachycardia and deceleration frequency were analyzed and correlated to immediate postnatal outcomes...
July 30, 2018: European Journal of Obstetrics, Gynecology, and Reproductive Biology
Robin J Reash
Mercury (Hg) is a neurotoxin that can cause debilitating effects to human and environmental receptors under high exposure conditions. For industrial and municipal point sources that discharge Hg, wastewater limitations on total mercury (THg) concentrations or loads are typical. While this regulatory practice provides simplicity for regulated industry and water resource agencies (re: analytical detection and reporting purposes), it ignores the important considerations of speciation and bioavailability. In this study water samples were collected from multiple power plant wastewater, simulated mixing zone, and ambient river locations (N = 10 to 20) and analyzed for bioavailable Hg (methylmercury and acid-labile mercury, or BHg) forms, THg, and dissolved Hg...
August 10, 2018: Integrated Environmental Assessment and Management
Hande E Cingil, Nicky C H Meertens, Ilja K Voets
Responsive materials, which can adapt and operate autonomously under dynamic conditions, are a stepping stone towards functional, life-like systems inspired by fueled self-assembly processes in nature. Complex coacervate core micelles (C3Ms) comprising oppositely charged macromolecules constitute a novel class of polymeric micelles ideally suited for use as responsive nanoscopic delivery vehicles of hydrophilic and hydrophobic cargo. To fully exploit their potential, it is important that the C3Ms form and fall apart in an autonomous fashion as orchestrated by dynamic cues in their environment...
August 10, 2018: Small
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