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oocyte specific

Amanda L Minogue, Michael R Tackett, Elnaz Atabakhsh, Genesis Tejada, Swathi Arur
Oocyte-specific miRNA function remains unclear in mice and worms because loss of Dgcr8 and Dicer from mouse and worm oocytes, respectively, does not yield oogenic defects. These data lead to several models: (a) miRNAs are not generated in oocytes; (b) miRNAs are generated but do not perform an oogenic function; (c) functional oocyte miRNAs are generated in a manner independent of these enzymes. Here, we test these models using a combination of genomic, expression and functional analyses on the C. elegans germline...
December 14, 2018: Nature Communications
Xingliang Wang, Andrias O O Reilly, Martin S Williamson, Alin M Puinean, Yihua Yang, Shuwen Wu, Yidong Wu
Glutamate-gated chloride channels (GluCls) are found only in invertebrates and mediate fast inhibitory neurotransmission. The structural and functional diversity of GluCls are produced through assembly of multiple subunits and via posttranscriptional alternations. Alternative splicing is the most common way to achieve this in insect GluCls and splicing occurs primarily at exons 3 and 9. As expression pattern and pharmacological properties of exon 9 alternative splices in invertebrate GluCls remain poorly understood, the cDNAs encoding three alternative splice variants (9a, 9b and 9c) of the PxGluCl gene from the diamondback moth Plutella xylostella were constructed and their pharmacological characterizations were examined using electrophysiological studies...
December 11, 2018: Insect Biochemistry and Molecular Biology
Julian A Poush, Nicolas A Blouin, Kristin R Di Bona, Vladimir Lažetić, David S Fay
RING-between-RING (RBR) E3 ubiquitin ligases are implicated in various developmental processes, and mutations in genes encoding RBR proteins HHARI/ARIH1 and Parkin are associated with human diseases. Here we show by phylogenetic analysis that the ARI1 family has undergone a dramatic expansion within the Caenorhabditis clade in recent history, a characteristic shared by some genes involved in germline development. We then examined the effects of deleting all ARI1 family members in the nematode Caenorhabditis elegans, which to our knowledge represents the first complete knockout of ARI1 function in a metazoan...
December 10, 2018: Scientific Reports
Yi Luan, Maxwell E Edmonds, Teresa K Woodruff, So-Youn Kim
Cancer therapy can cause off-target effects including ovarian damage, which may result in primary ovarian insufficiency in girls and premenopausal women. Loss of ovarian follicles within the ovarian reserve leads to ovarian endocrine dysfunction and impaired fertility. Cyclophosphamide (CPA), a commonly used chemotherapeutic and immunosuppressant agent, is a gonadotoxic agent that destroys ovarian cells by crosslinking DNA. To protect the ovary against CPA damage, we sought to precisely map the mechanism by which the ovarian reserve is depleted by CPA...
February 1, 2019: Journal of Endocrinology
C Jean, F Haghighirad, Y Zhu, M Chalbi, A Ziyyat, E Rubinstein, C Gourier, P Yip, J P Wolf, J E Lee, C Boucheix, V Barraud-Lange
STUDY QUESTION: Is JUNO protein present at the surface membrane of human oocytes and involved in the fertilisation process? SUMMARY ANSWER: JUNO protein is expressed on the plasma membrane of human oocytes and its inhibition by a monoclonal antibody completely blocks gamete fusion. WHAT IS KNOWN ALREADY: Fusion of gamete membranes is the culminating event of the fertilisation process, but its molecular mechanisms are poorly understood. Until now, three molecules have been shown to be essential: CD9 tetraspanin in the oocyte, Izumo1 protein on the sperm and Juno, its corresponding receptor on the oocyte...
December 5, 2018: Human Reproduction
D Haouzi, I Boumela, K Chebli, S Hamamah
Survival and cell death signals are crucial for mammalian embryo preimplantation development. However, the knowledge on the molecular mechanisms underlying their regulation is still limited. Mouse studies are widely used to understand preimplantation embryo development, but extrapolation of these results to humans is questionable. Therefore, we wanted to analyse the global expression profiles during early mouse and human development with a special focus on genes involved in the regulation of the apoptotic and survival pathways...
2018: BioMed Research International
Jun-Yu Ma, Li-Ying Yan, Zhen-Bo Wang, Shi-Ming Luo, William S B Yeung, Xiang-Hong Ou, Qing-Yuan Sun, Jie Qiao
BACKGROUND: The human oocyte transmits one set of haploid genome into female pronucleus (FPN) while discards the remaining genome into the first polar body (PB1) and the second polar body (PB2). The FPN genome carries an assembly of maternal and paternal genome that resulted from homologous recombination during the prophase of the first meiosis. However, how parental genome has been shuffled and transmitted is difficult to assess by analysing only the progeny's genome. OBJECTIVE: To assess meiotic chromatid recombination and segregation in human oocytes...
December 4, 2018: Journal of Medical Genetics
Zhiyun Shi, Juan Xu, Xuemei Jia
OBJECTIVE: To study the effect of Wdr1 deletion in germ cells on ovarian function of mice. METHODS: Oocyte-specific gene knockout mouse model was constructed by crossing Wdr1fl/fl female mice with Cre recombinase transgenic male mice which was driven by a germ cell-specific promoter. Wdr1fl/- ; Ddx4-Cre mice and control mice were sacrificed at 14 days, 28 days and 4 months after birth, whose ovaries were subjected to photography, paraffin sectioning and Hematoxylin-Eosin (HE) staining...
December 10, 2018: Zhonghua Yi Xue Yi Chuan Xue za Zhi, Zhonghua Yixue Yichuanxue Zazhi, Chinese Journal of Medical Genetics
Yukiko Nakamura, Makoto Kondo, Yoshihisa Koyama, Shoichi Shimada
The serotonin (5-hydroxytryptamine) type 3 (5-HT3 ) receptors are transmembrane ligand-gated ion channels. Although several 5-HT3 receptor agonists have been used as preclinical tools, SR 57227A is the most commonly used 5-HT3 receptor agonist with the ability to cross the blood brain barrier. However, the precise pharmacological profile of SR 57227A remains unclear. Therefore, we examined the pharmacological profile of SR 57227A at the 5-HT3 A and 5-HT3 AB receptors. We microinjected Xenopus laevis oocytes with human 5-HT3 A complementary RNA (cRNA) or a combination of human 5-HT3 A and human 5-HT3 AB cRNA and performed two electrode voltage clamp recordings of 5-HT3 A and 5-HT3 AB receptor current in the presence of SR 57227A...
December 1, 2018: Biochemical and Biophysical Research Communications
Arpana Rawat, Radha Chaube, Keerrikkattil P Joy
In the catfish Heteropneustes fossilis, three vasotocin (VT) receptor subtype genes, v1a1, v1a2, and v2a, were cloned and characterized previously. In the present study, using RNA probes, we localized the distribution of the gene transcripts in the brain-pituitary-gonadal (BPG) axis. The V1a-type receptor, v1a1 and v1a2, genes showed similar and overlapping distribution in the brain. The gene paralogs are distributed in the radial glial cells (RGCs) of the telencephalic ventricle and around the third ventricle in the hypothalamus and thalamus, olfactory tract, nucleus preopticus, nucleus lateralis tuberis, nucleus recessus lateralis and posterioris, nucleus saccus vasculosi, thalamic nuclei, habenular nucleus, habenular commissure, basal part of pineal stalk, accessory pretectal nucleus, optic tectum, corpus and valvula of the cerebellum, and facial and vagal lobes...
December 3, 2018: Fish Physiology and Biochemistry
Jing Xu, Maralee S Lawson, Fuhua Xu, Yongrui Du, Olena Y Tkachenko, Cecily V Bishop, Lucas Pejovic-Nezhat, David B Seifer, Jon D Hennebold
There is an increasing recognition that vitamin D plays important roles in female reproduction. Recent studies demonstrated that 1α,25-dihydroxyvitamin D3 (VD3), the biologically active form of vitamin D, improved ovarian follicle survival and growth in vitro . Therefore, we investigated the direct effects of VD3 at the specific preantral and antral stages of follicular development, and tested the hypothesis that vitamin D receptor (VDR) and enzymes critical for vitamin D biosynthesis are expressed in the primate ovary...
2018: Frontiers in Physiology
Yander L Diez, Claudia Sanjuan HernÁndez, Patrick Reygel, Paulien Roosen, Tom Artois
The first records of Polycystididae from Cuba are provided and discussed. In total nine species have been collected, five of which are new to science, one representing a new genus. Polycydora intermedia gen. n. sp. n. shows intermediate features between Polycystis Kölliker, 1845 and Paulodora Marcus, 1948. The ovaries are kidney shaped, with the oocytes arranged in a row, and lack the hard "nozzles" of Paulodora. A female bursa as in Polycystis is present. In P. intermedia gen. n. sp. n. lacks the strong muscle bulb at the male bursa and the accessory glands type I in the male atrium, which are present in Polycystis...
November 5, 2018: Zootaxa
Francesca Filippi, Fabio Martinelli, Alessio Paffoni, Marco Reschini, Francesco Raspagliesi, Edgardo Somigliana
PURPOSE: To evaluate the capacity of random antral follicle count (AFC), i.e., AFC recorded at any time during the menstrual cycle, to predict the number of retrieved mature oocytes in women with malignancies undergoing random start ovarian hyperstimulation METHODS: A consecutive series of 72 women with malignancies who underwent ovarian hyperstimulation aimed at egg freezing between July 2014 and December 2016 was retrospectively reviewed. A standardized random start protocol was used for all women...
November 26, 2018: Journal of Assisted Reproduction and Genetics
Qian-Qian Sha, Jia-Li Yu, Jing-Xin Guo, Xing-Xing Dai, Jun-Chao Jiang, Yin-Li Zhang, Chao Yu, Shu-Yan Ji, Yu Jiang, Song-Ying Zhang, Li Shen, Xiang-Hong Ou, Heng-Yu Fan
Meiotic resumption-coupled degradation of maternal transcripts occurs during oocyte maturation in the absence of mRNA transcription. The CCR4-NOT complex has been identified as the main eukaryotic mRNA deadenylase. In vivo functional and mechanistic information regarding its multiple subunits remains insufficient. Cnot6l , one of four genes encoding CCR4-NOT catalytic subunits, is preferentially expressed in mouse oocytes. Genetic deletion of Cnot6l impaired deadenylation and degradation of a subset of maternal mRNAs during oocyte maturation...
November 26, 2018: EMBO Journal
Nunzia Zanotta, Lorenzo Monasta, Kristina Skerk, Stefania Luppi, Monica Martinelli, Giuseppe Ricci, Manola Comar
PROBLEM: Cytokines have a significant role in the process of embryo implantation, trophoblast growth, and differentiation by modulating the immune and endocrine system. The aim of this study was to investigate the profile of a large set of cytokines in the cervico-vaginal washing of women undergoing IVF, to explore the association of these proteins with a good receptive endometrium. METHOD OF STUDY: A cohort of 155 women scheduled for IVF cycle was recruited. All patients were asymptomatic for genitourinary infections and had been screened for chlamydia, mycoplasma, and other bacterial infections...
November 26, 2018: American Journal of Reproductive Immunology: AJRI
Maria Giorgia Morselli, Monica Loiacono, Martina Colombo, Michele Mortarino, Gaia Cecilia Luvoni
To evaluate the ability of a 3D culture system in improving the nuclear and molecular competence of canine oocytes, barium alginate microcapsules were used for in vitro maturation (IVM) and the expression profile of one selected oocyte-secreted factor, the growth differentiation factor-9 (GDF-9) was analysed. In Experiment I, canine grade I cumulus-oocyte complexes (COCs) were in vitro matured in 3D microcapsules in a controlled atmosphere for 72 hr, and meiosis resumption rates were compared to those of oocytes cultured in traditional 2D microdrops of medium...
November 2018: Reproduction in Domestic Animals, Zuchthygiene
Yaoyao Zhang, Zhiqiang Yan, Qingyuan Qin, Vicki Nisenblat, Hsun-Ming Chang, Yang Yu, Tianren Wang, Cuiling Lu, Ming Yang, Shuo Yang, Ying Yao, Xiaohui Zhu, Xi Xia, Yujiao Dang, Yixin Ren, Peng Yuan, Rong Li, Ping Liu, Hongyan Guo, Jinsong Han, Haojie He, Kun Zhang, Yiting Wang, Yu Wu, Meng Li, Jie Qiao, Jie Yan, Liying Yan
The dynamic transcriptional regulation and interactions of human germlines and surrounding somatic cells during folliculogenesis remain unknown. Using RNA sequencing (RNA-seq) analysis of human oocytes and corresponding granulosa cells (GCs) spanning five follicular stages, we revealed unique features in transcriptional machinery, transcription factor networks, and reciprocal interactions in human oocytes and GCs that displayed developmental-stage-specific expression patterns. Notably, we identified specific gene signatures of two cell types in particular developmental stage that may reflect developmental competency and ovarian reserve...
November 15, 2018: Molecular Cell
D Abadjieva, M Petkova, S Grigorova, E Kistanova
The aim of this study was to assess the biological effect of the product Jodis Concentrate (JC) on the rabbit ovaries by evaluating the folliculogenesis and expression of oocyte-specific growth differentiation factor 9 (GDF9).The experiment was conducted with 30 female two month old New Zealand rabbits that were the F1 offspring born to mothers differently treated with Jodis concentrate. The control group (n=10), consisted of F1 offspring born to mothers without iodine treatment, and was not supple- mented with JC...
September 2018: Polish Journal of Veterinary Sciences
Jorge Merlet, Karinna Rubio-Peña, Sara Al Rawi, Vincent Galy
The nematode C. elegans represents a powerful experimental system with key properties and advantages to study the mechanisms underlying mitochondrial DNA maternal inheritance and paternal components sorting. First, the transmission is uniparental and maternal as in many animal species; second, at fertilization sperm cells contain both mitochondria and mtDNA; and third, the worm allows powerful genetics and cell biology approaches to characterize the mechanisms underlying the uniparental and maternal transmission of mtDNA...
November 23, 2018: Advances in Anatomy, Embryology, and Cell Biology
Julia Uraji, Kathleen Scheffler, Melina Schuh
Gametes undergo a specialized and reductional cell division termed meiosis. Female gametes (oocytes) undergo two rounds of meiosis; the first meiotic division produces the fertilizable egg, while the second meiotic division occurs upon fertilization. Both meiotic divisions are highly asymmetric, producing a large egg and small polar bodies. Actin takes over various essential function during oocyte meiosis, many of which commonly rely on microtubules in mitotic cells. Specifically, the actin network has been linked to long-range vesicle transport, nuclear positioning, spindle migration and anchorage, polar body extrusion and accurate chromosome segregation in mammalian oocytes...
November 22, 2018: Journal of Cell Science
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