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Colistin resistant klebsiella

Yu-Han Liu, Shu-Chen Kuo, Bing-Yu Yao, Zih-Syun Fang, Yi-Tzu Lee, Yuan-Chih Chang, Te-Li Chen, Che-Ming Jack Hu
Amidst the ever-rising threat of antibiotics resistance, colistin, a decade-old antibiotic with lingering toxicity concern, is increasingly prescribed to treat many drug-resistant, gram-negative bacteria. With the aim of improving the safety profile while preserving the antimicrobial activity of colistin, a nanoformulation is herein developed through coacervate complexation with polyanionic peptides. Upon controlled mixing of cationic colistin with polyglutamic acids, formation of liquid coacervates was demonstrated...
October 10, 2018: Acta Biomaterialia
Vinícius Victor Lorenzoni, Franciéli da Costa Rubert, Roberta Filipini Rampelotto, Rosmari Hörner
INTRODUCTION: The spread of multidrug-resistant Gram-negative bacilli is a health threat, limiting therapeutic options and increasing morbimortality rates. METHODS: This study aimed to evaluate the antimicrobial susceptibility profile of 1805 Klebsiella pneumoniae isolates collected from Hospital Universitário de Santa Maria between January 2015 and December 2016. RESULTS: Resistance to colistin (239.3%), meropenem (74.2%), ciprofloxacin (68%), gentamicin (35...
September 2018: Revista da Sociedade Brasileira de Medicina Tropical
Mutasim E Ibrahim
To determine the distribution and resistance profiles of Gram-negative bacteria (GNB) in intensive care units (ICUs) at King Abdullah Hospital in Bisha, Saudi Arabia. Methods: A record based retrospective study was conducted from December 2016 to January 2018. In total, 3736 non-duplicate clinical specimens from the general intensive care unit (ICU), neonatal ICU (NICU), and coronary CU (CCU) were analyzed for pathogens. Results: Of 3736 specimens, 9.6% (358) were positive for pathogens, and GNB constituted the majority (290/358; 81%)...
October 2018: Saudi Medical Journal
Lu Liu, Yu Feng, Yiyi Hu, Mei Kang, Yi Xie, Zhiyong Zong
Klebsiella grimontii is a newly identified species closely related to Klebsiella oxytoca , but carbapenem resistance was not identified in the species before. We found a carbapenem-resistant K. oxytoca -like clinical strain, WCHKG020121. The strain was subjected to whole genome sequencing using Illumina HiSeq X10. The precise species identification was established based on average nucleotide identity (ANI) and in silico DNA-DNA hybridization (isDDH) between strain WCHKG020121 and type strains of Klebsiella species...
2018: Frontiers in Microbiology
Hugo Barragán-Prada, Paula Ruiz-Hueso, Ana P Tedim, Fernando González-Candelas, Juan Carlos Galán, Rafael Cantón, María-Isabel Morosini
Dissemination of multidrug-resistant Klebsiella pneumoniae in the hospital environment represents a primary target of resistance containment and stewardship programs. At present, polymyxins, mostly in combination, exemplify a last-resort alternative. Colistin-resistant K. pneumoniae isolates harboring OXA-48 plus CTX-M-15 (n = 21) with the simultaneous colistin-susceptible counterparts (n = 9) were recovered from 14 hospitalized patients (January 2014-January 2015) admitted in different wards. In most cases, patients had not previously received colistin...
September 8, 2018: Diagnostic Microbiology and Infectious Disease
Hanane Yousfi, Linda Hadjadj, Iman Dandachi, Rym Lalaoui, Adil Merah, Kamel Amoura, Ahlem Dahi, Mazouz Dekhil, Naima Messalhi, Seydina M Diene, Sophie Baron, Jean-Marc Rolain
This study investigates the molecular mechanisms of colistin and carbapenem resistance in Klebsiella pneumoniae ST101 strains. The three K. pneumoniae carried blaCTX-M-15 , blaTEM-183 , and blaSHV-106 genes and two coharbored blaOXA-48 . As for colistin resistance, the isolates had amino acid substitutions in PmrA/B and a truncated mgrB gene in one isolate.
September 25, 2018: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
Kuntaman Kuntaman, Katsumi Shigemura, Kayo Osawa, Koichi Kitagawa, Koharu Sato, Naoki Yamada, Kento Nishimoto, Fukashi Yamamichi, Dadik Rahardjo, Usman Hadi, Ni Made Mertaniasih, Shohiro Kinoshita, Masato Fujisawa, Toshiro Shirakawa
OBJECTIVES: To explore the occurrence and characterization of carbapenemase-producing pathogens among carbapenem-resistant Gram-negative bacilli isolated from hospitalized patients with urinary tract infection in Indonesia. METHODS: This was a study promoted by the Japanese-Indonesian collaborative research program in the Japan Initiative for Global Research Network on Infectious Diseases. Bacterial pathogens were prospectively isolated from urine specimens of hospitalized urinary tract infection patients at Dr...
September 25, 2018: International Journal of Urology: Official Journal of the Japanese Urological Association
Marta Ferranti, Elisabetta Schiaroli, Melissa Ida Palmieri, Antonella Repetto, Anna Vecchiarelli, Daniela Francisci, Antonella Mencacci, Claudia Monari
The global dissemination of carbapenemase-producing Enterobacteriaceae (CPE) is of great concern for public health. These bacteria have the potential for rapid dissemination in healthcare settings and cause infections associated with high rates of morbidity and mortality. A total of 221 carbapenem non-susceptible Enterobacteriaceae isolates were collected from patients admitted to an Italian general hospital from January 2016 to March 2017. Among these isolates, 78.3% were carbapenemase producers: 96% were positive for the blaKPC gene and the remainder for the blaVIM gene (allelic variant VIM-1)...
September 25, 2018: New Microbiologica
Amira A Moawad, Helmut Hotzel, Heinrich Neubauer, Ralf Ehricht, Stefan Monecke, Herbert Tomaso, Hafez M Hafez, Uwe Roesler, Hosny El-Adawy
Background: Poultry remains one of the most important reservoir for zoonotic multidrug resistant pathogens. The global rise of antimicrobial resistance in Gram-negative bacteria is of reasonable concern and demands intensified surveillance. Methods: In 2016, 576 cloacal swabs were collected from 48 broiler farms located in five governorates in northern Egypt. Isolates of Enterobacteriaceae could be cultivated on different media and were identified by MALDI-TOF MS and PCR...
2018: Gut Pathogens
Abdul Ghafur, Chaitra Shankar, Palani GnanaSoundari, Manigandan Venkatesan, M A Thirunarayanan, Deepa Mani, Balaji Veeraraghavan
OBJECTIVES: Multiple earlier publications on detection of mcr-1 in animals and human isolates, strongly suggested an underlying route of food chain transmission of colistin resistance (Col-R). Aim of our study was to investigate the presence of Col-R bacteria in Indian food samples and to identify the underlying mechanisms conferring colistin resistance. METHODS: Raw food materials including poultry meat, mutton meat, fish and vegetables collected from food outlets of Chennai, India, were processed to identify Col-R bacteria using Eosin Methylene Blue agar supplemented with colistin...
September 20, 2018: Journal of Global Antimicrobial Resistance
Govindan Rajivgandhi, Thillaichidambaram Muneeswaran, Muthuchamy Maruthupandy, Chockalingam Muthiah Ramakritinan, Kandasamy Saravanan, Vilwanathan Ravikumar, Natesan Manoharan
The aim of the current study is to identify bioactive compound from marine endophytic actinomycetes (MEA) isolated from Gulf of Mannar region, Southeast coast of India. Among the isolated actinomycetes, strain GRG 4 exhibited excellent ability to inhibit isolated colistin resistant (CR) Pseudomonas aeruginosa (P. aeruginosa) and Klebsiella pneumoniae (K. pneumoniae), which is a emerging threat to the world. The strain was identified as Streptomyces coeruleorubidus GRG 4 (KY457708), based on morphological, biochemical, phenotypic and genotypic characters...
September 19, 2018: Microbial Pathogenesis
Raad Jasim, Mark A Baker, Yan Zhu, Meiling Han, Elena K Schneider-Futschik, Maytham Hussein, Daniel Hoyer, Jian Li, Tony Velkov
In the present report we characterized the outer membrane proteome, genomic, and lipid A remodelling changes following the evolution of a colistin-susceptible K. pneumoniae ATCC 13883 strain into an extremely colistin-resistant strain. Lipid A profiling revealed the outer membrane of the colistin-susceptible strain is decorated primarily by hexa- and hepta-acylated lipid A species and a minor tetra-acylated species. In the lipid A profile of the extremely colistin-resistant strain, in addition to the aforementioned lipid A species, the obligatory 4-amino-4-deoxy-l-arabinose modification of the hexa-acylated lipid A was detected...
October 5, 2018: ACS Infectious Diseases
Wadha Alfouzan, Rita Dhar, David P Nicolau
Limited data are available on susceptibilities of these organisms to some of the recently made accessible antimicrobial agents. The in vitro activities of newer antibiotics, such as, ceftolozane/tazobactam (C/T) and ceftazidime/avibactam (CZA) along with some "older" antibiotics, for example fosfomycin (FOS) and colistin (CL) were determined against selected strains (resistant to ≥ 3 antimicrobial agents) of Escherichia coli , Klebsiella pneumoniae , and Pseudomonas aeruginosa . Minimum inhibitory concentrations (MIC) were determined by Clinical and Laboratory Standards Institute microbroth dilution...
September 17, 2018: Pathogens
A Kumar, L Biswas, N Omgy, K Mohan, V Vinod, A Sajeev, P Nair, S Singh, R Biswas
OBJECTIVE: Mutations in mgrB, phoP/phoQ, pmrA, pmrB, pmrC, and crrABC regulatory systems have been found responsible for colistin resistance. The aim of our study was to investigate the role of alteration in mgrB gene and plasmid mediate mcr-1 and mcr-2 genes as a source of colistin resistance in 17 non duplicate Klebsiella pneumoniae clinical isolates. METHODS: All isolates classified as resistant to colistin by VITEK 2 system (BioMerieux, Marcy I' Etoile, France) were included...
September 14, 2018: Revista Española de Quimioterapia: Publicación Oficial de la Sociedad Española de Quimioterapia
Verónica E Álvarez, Josefina Campos, Antonio Galiana, Graciela Borthagaray, Daniela Centrón, Carolina Márquez Villalba
OBJECTIVES: Since KPC-2-producing Klebsiella pneumoniae are associated with successful dissemination of a major clone, defined as sequence type 258 (ST258), the aim of this study was to perform whole-genome sequencing (WGS) of the first colistin-resistant K. pneumoniae strain (Kpn666) carrying blaKPC-2 identified in Uruguay in 2011 in order to identify genomic and phylogenetic traits. METHODS: WGS of strain Kpn666 isolated from an asymptomatic urinary tract infection was performed using Illumina MiSeq, and de novo assembly was performed using SPADES v...
September 10, 2018: Journal of Global Antimicrobial Resistance
K Szymanek-Majchrzak, A Mlynarczyk, R Kuthan, A Sawicka-Grzelak, K Majchrzak, D Kawecki, M Kosieradzki, M Durlik, D Deborska-Materkowska, L Paczek, G Mlynarczyk
BACKGROUND: The first New Delhi metallo-beta-lactamase (NDM)-producing bacteria were isolated in 2008 in the world, and in 2011 in Poland. Due to the high clonal diversity (17 types) of their blaNDM gene, encoded on (Tn125-like) mobile genetic elements, these strains usually exhibit resistance to nearly all available antibiotics, which is particularly dangerous for organ transplant recipients. PURPOSE: To assess of the prevalence of Gram-negative NDM-positive bacilli in surgery/transplantation wards of a teaching hospital in Warsaw and to ascertain the significance of screening tests on the rates and nature of colonization...
September 2018: Transplantation Proceedings
Farah Ben Tanfous, Anis Raddaoui, Yosra Chebbi, Wafa Achour
The aim of our retrospective study was to investigate the resistance determinants and the genetic relatedness of colistin-resistant Klebsiella pneumoniae isolates in the National Bone Marrow Transplant Center of Tunis (NBMTC) between 2002 and 2013. Colistin-resistant K. pneumoniae isolates were investigated. The minimal inhibitory concentration (MIC) of colistin was assessed using the UMIC broth microdilution kit. All isolates were screened by PCR assay for resistance genes: mcr-1, blaCTX-M1 , blaSHV , blaTEM , blaOXA-1 blaOXA-48 , blaKPC , blaIMP , blaVIM , blaNDM , blaGES , blaIMI and AmpC group as well as class 1, 2 and 3 integrons...
August 31, 2018: International Journal of Antimicrobial Agents
Xiaojuan Wang, Chunjiang Zhao, Henan Li, Hongbin Chen, Longyang Jin, Zhanwei Wang, Kang Liao, Ji Zeng, Xiuli Xu, Yan Jin, Danhong Su, Wenen Liu, Zhidong Hu, Bin Cao, Yunzhuo Chu, Rong Zhang, Yanping Luo, Bijie Hu, Hui Wang
To dynamically investigate the distribution and antimicrobial resistance profiles of bacteremia pathogens isolated from different regions in China in 2011, 2013 and 2016. Non-repetitive isolates from nosocomial bloodstream infections were retrospectively collected and detected for antimicrobial susceptibility tests (AST) by agar dilution or microbroth dilution methods. Whonet 5.6 was used to analyze the AST data. Among 2 248 isolates, 1 657 (73.7%) were Gram-negative bacilli and 591 (26.3%) were Gram-positive cocci...
August 25, 2018: Sheng Wu Gong Cheng Xue Bao, Chinese Journal of Biotechnology
Tatsuya Tada, Kohei Uechi, Isamu Nakasone, Masashi Nakamatsu, Kazuhito Satou, Takashi Hirano, Teruo Kirikae, Jiro Fujita
The mcr-1 gene encodes a phosphoethanolamine transferase that confers resistance to colistin by transferring phosphoethanolamine to lipid A. A 33-kb IncX4 plasmid harboring mcr-1 was detected in a Klebsiella pneumoniae isolate and two Escherichia coli isolates, and a 66-kb IncI2 plasmid was detected in three E. coli isolates in hospitals in Okinawa, Japan.
October 2018: International Journal of Infectious Diseases: IJID
Taher Uz Zaman, Maha Albladi, Mohammed Ismail Siddique, Sameera M Aljohani, Hanan H Balkhy
Background: In Klebsiella pneumoniae, mgrB and components of pmrHFIJKLM operon play a major role in colistin resistance. Methods: We analyzed 23 nonduplicating colistin-resistant K. pneumoniae isolates, collected during the years 2011-2015, for the possible mechanism underlying their nonsusceptibility to colistin. Isolates were tested for their minimum inhibitory concentrations and antibiotic resistance determinants and genotyped by multilocus sequence typing (MLST)...
2018: Infection and Drug Resistance
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