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https://www.readbyqxmd.com/read/30538497/the-effect-of-piperlongumine-on-endothelial-and-lung-adenocarcinoma-cells-with-regulated-expression-of-profilin-1
#1
Maciej Gagat, Marta Hałas-Wiśniewska, Wioletta Zielińska, Magdalena Izdebska, Dariusz Grzanka, Alina Grzanka
Purpose: The aim of the study was to evaluate the effect of piperlongumine (2 and 4 µM) on endothelial EA.hy926 and lung adenocarcinoma A549 cells with regulated expression of profilin-1 (PFN1). Material and methods: The cytotoxicity of alkaloid was evaluated by MTT assay, while cell death was assessed using double staining with annexin V and propidium iodide. Subsequently, the level of PFN1 1) upregulation in EA.hy926 endothelial cells and 2) downregulation in A549 lung adenocarcinoma cells...
2018: OncoTargets and Therapy
https://www.readbyqxmd.com/read/30537737/the-transient-receptor-potential-channel-vanilloid-5-induces-chondrocyte-apoptosis-via-ca2-camkii-dependent-mapk-and-akt-mtor-pathways-in-a-rat-osteoarthritis-model
#2
Yingliang Wei, Zhaofeng Jin, He Zhang, Shang Piao, Jinghan Lu, Lunhao Bai
BACKGROUND/AIMS: Chondrocyte apoptosis is a central pathological feature of cartilage in osteoarthritis (OA). Accumulating evidence suggests that calcium ions (Ca2+) are an important regulator of apoptosis. Previously, we reported that the transient receptor potential channel vanilloid (TRPV5) is upregulated in monoiodoacetic acid (MIA)-induced OA articular cartilage. METHODS: The protein levels of TRPV5, phosphorylated Ca2+/calmodulin-dependent kinase II (p-CaMKII), and total CaMKII were detected in vivo using western blotting techniques...
2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/30537677/dexamethasone-induces-osteoblast-apoptosis-through-ros-pi3k-akt-gsk3%C3%AE-signaling-pathway
#3
Shuang Deng, Guo Dai, Sen Chen, Zhigang Nie, Jianlin Zhou, Hongsong Fang, Hao Peng
BACKGROUND: Osteoblasts play important roles in the process of osteogenesis and prevention of osteonecrosis. Dexamethasone (Dex), a type of glucocorticoids (GCs), induces apoptosis of osteoblasts and leads to the occurrence of non-traumatic osteonecrosis. This study aimed to explore the effects of phosphatidylinositol 3-kinase/Protein kinase 3 (PI3K/AKT) and glycogen synthase kinase 3β (GSK3β) on Dex-induced osteoblasts apoptosis. METHODS: Viabilities, proliferation, and apoptosis of primary osteoblasts and pre-osteoblast MC3T3-E1 cells after Dex treatment were detected using cell counting kit-8 (CCK-8) assay, 5-bromo-2'-deoxyuridine (BrdU) incorporation assay, FITC-Annexin V/PI staining and western blotting, respectively...
December 8, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/30536399/in-vitro-modulatory-effect-of-dehydroepiandrosterone-sulfate-on-apoptosis-and-expression-of-apoptosis-related-genes-in-patients-with-systemic-lupus-erythematosus
#4
Bahman Yousefi, Maryam Rastin, Mohammad Reza Hatef, Jaleh Shariati, Reza Alimohammadi, Mahmoud Mahmoudi
OBJECTIVES: Systemic lupus erythematosus‌ (SLE) is an autoimmune disease characterized by hyperactive B cells that produce various autoantibodies. Sex hormones have been documented to influence the development of SLE, in which women with SLE have low plasma level of dehydroepiandrosterone sulfate (DHEAS). A strong conclusion about the effect of DHEAS on apoptosis in SLE patients has not been provided. The aim of this study was to assess apoptotic effects of DHEAS on peripheral blood lymphocytes (PBLs) from SLE patients...
December 7, 2018: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/30536377/loss-of-lgr4-inhibits-differentiation-migration-and-apoptosis-and-promotes-proliferation-in-bone-mesenchymal-stem-cells
#5
Peng Sun, Kunhang Jia, Chunbing Zheng, Xinlei Zhu, Jing Li, Liang He, Stefan Siwko, Feng Xue, Mingyao Liu, Jian Luo
The key signaling networks regulating bone marrow mesenchymal stem cells (BMSCs) are poorly defined. Lgr4, which belongs to the leucine-rich repeat-containing G protein-coupled receptor (LGR) family, is widely expressed in multiple tissues from early embryogenesis to adulthood. We investigated whether Lgr4 functions in BMSCs and in osteogenesis, adipogenesis, and skeletal myoblasts, using mice with a β-geo gene trap inserted into the Lgr4 gene. Abundant Lgr4 expression was detected in skeletal, adipose and muscular tissue of Lgr4+/- mice at E16...
December 7, 2018: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/30536061/annexin-a1-mimetic-peptide-ac2-26-inhibits-sepsis-induced-cardiomyocyte-apoptosis-through-lxa4-pi3k-akt-signaling-pathway
#6
Li Zhang, Yan-Lei Zheng, Rong-Hua Hu, Li Zhu, Chen-Chen Hu, Fei Cheng, Shi Li, Jian-Guo Li
The aim of the present study was to explore the effects of annexin A1 (ANXA1) mimetic peptide AC2-26 on sepsis-induced cardiomyocyte apoptosis in vivo and in vitro and the underlying mechanisms. In the in vivo study, a rat septic model was established by the cecal ligation and puncture (CLP). The rats were divided into control group, sepsis group and AC2-26 group. The rats in the AC2-26 group were intraperitoneally injected with AC2-26 (1 mg/kg) 2 h before CLP, and those in the control group and sepsis group were injected with the same volume of normal saline...
December 2018: Current medical science
https://www.readbyqxmd.com/read/30535477/cryptotanshinone-induces-reactive-oxygen-species%C3%A2-mediated-apoptosis-in-human-rheumatoid-arthritis-fibroblast%C3%A2-like-synoviocytes
#7
Hu-Nan Sun, Ying-Hua Luo, Ling-Qi Meng, Xian-Ji Piao, Yue Wang, Jia-Ru Wang, Hao Wang, Yi Zhang, Jin-Qian Li, Wan-Ting Xu, Yang Liu, Yu Zhang, Tong Zhang, Ying-Hao Han, Mei-Hua Jin, Gui-Nan Shen, Yan-Qing Zang, Long-Kui Cao, Dong-Jie Zhang, Cheng-Hao Jin
The present study investigated the mechanisms of apoptosis induced by cryptotanshinone (CT) in human rheumatoid arthritis fibroblast‑like synoviocytes (RA‑FLSs). Cell Counting kit‑8 assay was performed to determine the cytotoxic effects of CT in human RA‑FLSs, including primary RA‑FLS, HFLS‑RA and MH7A cells, and in HFLS cells derived from normal synovial tissue. Annexin V‑FITC/PI staining was used to detect the apoptotic effects of CT in HFLS‑RA and MH7A cells. Flow cytometry was performed to detect the apoptotic and reactive oxygen species (ROS) levels induced by CT in HFLS‑RA cells...
December 3, 2018: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/30535471/microrna-497-inhibits-multiple-myeloma-growth-and-increases-susceptibility-to-bortezomib-by-targeting-bcl-2
#8
Faqing Tian, Yong Zhan, Wei Zhu, Juheng Li, Meiqin Tang, Xiaohui Chen, Jian Jiang
Multiple myeloma (MM) is a common severe hematopoietic malignancy occuring in aged population. MicroRNA (miR)‑497 was previously reported to contribute to the apoptosis of other cell types, presumably through targeting B‑cell lymphoma 2 (Bcl‑2). In the present study, miRNA and protein expression levels were detected by reverse transcription‑quantitative polymerase chain reaction and western blot analyses, respectively. The cell proliferation and viability was measured using 3‑(4,5‑dimethylthiazol‑2‑yl)‑2, 5‑diphenyltetrazolium bromide and plate clonality assays, and the cell growth cycle was measured with a flow cytometer...
December 7, 2018: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/30531680/curcumin-mediated-induction-of-apoptosis-in-human-glioma-chme-cells
#9
Yunqiang Zhang, Lijun Tu, Xiuhong Zhou, Bin Li
BACKGROUND Curcumin has clear anti-tumor activity in various carcinomas. It regulates various signaling pathways like Wnt/β-catenin and JAK2/STAT3, which play vital roles in cell proliferation of several carcinomas, but to the best of our knowledge, there are currently no published reports on human glioma CHME cells. Therefore, the aim of this study was to explore the effect of curcumin on human glioma CHME cells. MATERIAL AND METHODS The CHME cell line was purchased from American Type Culture Collection (ATCC)...
December 10, 2018: Medical Science Monitor Basic Research
https://www.readbyqxmd.com/read/30530178/structure-activity-relationships-of-2%C3%A2-quinolinecarboxaldehyde-thiosemicarbazone-gallium-iii-complexes-with-potent-and-selective-anticancer-activity
#10
Wanbao Cao, Jinxu Qi, Kun Qian, Liang Tian, Zhen Cheng, Yihong Wang
Six gallium(III) complexes (Ga1-Ga6) with 2‑quinolinecarboxaldehyde thiosemicarbazone analogues were synthesized and characterized. These gallium(III) complexes exhibited potent anticancer activity and exceeded that of the corresponding metal free ligands. Importantly, these gallium(III) complexes have a strong selectivity for tumor cells. Through the study of cellular mechanisms, we have found that the lipophilicity of ligands is closely linked to the antitumor activity of gallium(III) complexes. Additionally, we have chosen Ga6 with the best anti-tumor activity to study the mechanism of apoptosis...
November 28, 2018: Journal of Inorganic Biochemistry
https://www.readbyqxmd.com/read/30530072/new-tirucallane-triterpenoids-from-picrasma-quassioides-with-their-potential-antiproliferative-activities-on-hepatoma-cells
#11
Wen-Yu Zhao, Jing-Jie Chen, Chun-Xin Zou, Ying-Ying Zhang, Guo-Dong Yao, Xiao-Bo Wang, Xiao-Xiao Huang, Bin Lin, Shao-Jiang Song
Seven new tirucallane-type triterpenoids (1-7), kumuquassin A-G, along with 20 known analogues (8-27) were isolated from the stems of Picrasma quassioides. The structures and the absolute configurations of new compounds were elucidated by spectroscopic data, electronic circular dichroism (ECD) spectroscopic analyses and quantum ECD calculations. Notably, kumuquassin A (1) contains a rare Δ17, 20 double bond, kumuquassin B (2) is the first example of tirucallane triterpenoid possessing a 5/3 biheterocyclic ring system at the side chain...
November 28, 2018: Bioorganic Chemistry
https://www.readbyqxmd.com/read/30528439/induction-of-human-aortic-myofibroblast-mediated-extracellular-matrix-dysregulation-a-potential-mechanism-of-fluoroquinolone-associated-aortopathy
#12
David G Guzzardi, Guoqi Teng, Sean Kang, Patrick J Geeraert, Simranjit S Pattar, Daniyil A Svystonyuk, Darrell D Belke, Paul W M Fedak
OBJECTIVES: Fluoroquinolone (FQ) antibiotics are associated with adverse aortic clinical events. We assessed human aortic myofibroblast-mediated extracellular matrix (ECM) dysregulation as a possible cellular mechanism underlying FQ-associated aortopathy. METHODS: Human aortic myofibroblasts were isolated from patients with aortopathy undergoing elective ascending aortic resection (N = 9). The capacity for extracellular matrix degradation in cells exposed to FQ was assessed by multiplex analysis of secreted matrix metalloproteinases relative to tissue inhibitors of matrix metalloproteinases (TIMPs)...
September 21, 2018: Journal of Thoracic and Cardiovascular Surgery
https://www.readbyqxmd.com/read/30524652/metallothionein-i-ii-reduces-oxidative-damage-and-apoptosis-after-traumatic-spinal-cord-injury-in-rats
#13
Camilo Rios, Iván Santander, Marisela Méndez-Armenta, Concepción Nava-Ruiz, Sandra Orozco-Suárez, Marcela Islas, Verónica Barón-Flores, Araceli Diaz-Ruiz
After spinal cord injury (SCI), some self-destructive mechanisms start leading to irreversible neurological deficits. It is known that oxidative stress and apoptosis play a major role in increasing damage after SCI. Metallothioneins I and II (MT) are endogenous peptides with known antioxidant, neuroprotective capacities. Taking advantage of those capacities, we administered exogenous MT to rats after SCI in order to evaluate the protective effects of MT on the production of reactive oxygen species (ROS) and lipid peroxidation (LP), as markers of oxidative stress...
2018: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/30523224/-effect-of-parkinson-s-disease-relevant-protein-dj-1-on-cell-proliferation-apoptosis-invasion-and-migration-in-human-osteosarcoma-cells
#14
Hongwei Li, Xuchang Hu, Bing Ma, Haihong Zhang
To investigate the effect of Parkinson's disease related protein DJ-1 on the cell proliferation, apoptosis, invasion and migration in human osteosarcoma cells and the underlying molecular mechanisms. 
 Methods: The protein expression levels of DJ-1 were detected in human osteosarcoma cell lines (MG-63, Saos-2, and U2OS) and human osteoblast cell line hFOB1.19 with or without deficiency in phosphatase and tensin homolog deleted from chromosome 10 (PTEN) were detected by Western blot. Osteosarcoma cells were treated with DJ-1 siRNA, and then the protein expression levels of DJ-1 were detected by Western blot...
October 28, 2018: Zhong Nan da Xue Xue Bao. Yi Xue Ban, Journal of Central South University. Medical Sciences
https://www.readbyqxmd.com/read/30521543/human-bone-marrow-contains-high-levels-of-extracellular-vesicles-with-a-tissue-specific-subtype-distribution
#15
Andreas Rank, Rienk Nieuwland, Anton Köhler, Cordula Franz, Johanna Waidhauser, Bettina Toth
INTRODUCTION: Extracellular vesicles (EV) are shed from a broad variety of cells and play an important role in activation of coagulation, cell to cell interaction and transport of membrane components. They are usually measured as circulating EV in peripheral blood (PB) and other body fluids. However, little is known about the distribution, presence and impact of EV and their subpopulations in bone marrow (BM). In our study, we focused on the analysis of different EV subtypes in human BM as compared to EV subsets in PB...
2018: PloS One
https://www.readbyqxmd.com/read/30507084/in-vitro-cytotoxicity-of-folate-silica-gold-nanorods-on-mouse-acute-lymphoblastic-leukemia-and-spermatogonial-cells
#16
Neda Eslahi, Ali Shakeri-Zadeh, Khadijeh Ashtari, Vahid Pirhajati-Mahabadi, Tahereh Tohidi Moghadam, Ronak Shabani, Kamran Kamrava, Zahra Madjd, Chad Maki, Hamid Reza Asgari, Morteza Koruji
Objective: The purpose of this study was to evaluate in vitro cytotoxicity of gold nanorods (GNRs) on the viability of spermatogonial cells (SSCs) and mouse acute lymphoblastic leukemia cells (EL4s). Materials and Methods: In this experimental study, SSCs were isolated from the neonate mice, following enzymatic digestion and differential plating. GNRs were synthesized, then modified by silica and finally conjugated with folic acid to form F-Si-GNRs. Different doses of F-Si-GNRs (25, 50, 75, 100, 125 and 140 μM) were used on SSCs and EL4s...
April 2019: Cell Journal
https://www.readbyqxmd.com/read/30507008/targeting-of-high-mobility-group-a2-by-small-interfering-rna-loaded-nanoliposome-induced-apoptosis-and-migration-inhibition-in-gastrointestinal-cancer-cells
#17
Ali Mohammadi, Behzad Mansoori, Pouria Savadi, Vahid Khaze, Mahsa Minouei, Nigel A J McMillan, Somayeh Hallaj-Nezhadi, Behzad Baradaran
BACKGROUND: Considering the complex nature of gastrointestinal cancer, different methods including surgery, radiotherapy, and chemotherapy are considered for the treatment. Novel strategies including silencing of oncogenes using safe delivery systems could be considered as a novel approach in colorectal cancer treatment. The aim of this study was to investigate the silencing effect of high mobility group A2 (HMGA2) small interfering RNA (siRNA)-loaded nanoliposomes on gastrointestinal cancers...
December 2, 2018: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/30506718/microrna-193a-5p-inhibits-migration-of-human-ht-29-colon-cancer-cells-via-suppression-of-metastasis-pathway
#18
Naghmeh Shirafkan, Navid Shomali, Tohid Kazemi, Dariush Shanehbandi, Mehri Ghasabi, Elham Baghbani, Maziar Ganji, Vahid Khaze, Behzad Mansoori, Behzad Baradaran
PURPOSE: Altered expression of microRNAs (miRNAs) is indicated strongly in colorectal cancer (CRC). This study aims to evaluate the inhibitory role of miR-193a-5p on epithelial-mesenchymal transition markers in CRC lines. The cellular effects and potential mechanisms of miR-193a-5p were also examined. METHODS: Quantitative reverse-transcription polymerase chain reaction (RT-PCR) was performed to determine the expression of miR-193a-5p in three CRC cell lines (HCT-116, SW-480, and HT-29) and its impact on metastasis-related genes ( vimentin and CXCR4) before and after mimic transfection...
December 2, 2018: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/30504377/glutamine-deprivation-enhances-acetyl-coa-carboxylase-inhibitor-induced-death-of-human-pancreatic-cancer-cells
#19
Koji Nishi, Mina Suzuki, Noriko Yamamoto, Ayako Matsumoto, Yumiko Iwase, Keishi Yamasaki, Masaki Otagiri, Nagahiko Yumita
BACKGROUND/AIM: Acetyl-CoA carboxylase (ACC) is a rate-limiting enzyme in fatty acid synthesis. In this study, we investigated the effect of ACC inhibition on survival of pancreatic cancer cells. MATERIAL AND METHODS: AsPC-1, BxPC-3 and PANC-1 were used as human pancreatic cancer cell lines. 5-(etradecyloxy)-2-furoic acid (TOFA) and bis-2-(5-phenylacetamido-1,2,4-thiadiazol-2-yl) ethyl sulfide (BPTES) were used as inhibitors of ACC and glutaminase (GLS) respectively...
December 2018: Anticancer Research
https://www.readbyqxmd.com/read/30500850/proteomic-analysis-of-liver-tissue-from-dogs-with-chronic-hepatitis
#20
Yuri A Lawrence, Lawrence J Dangott, Aline Rodrigues-Hoffmann, Jörg M Steiner, Jan S Suchodolski, Jonathan A Lidbury
Chronic hepatitis is the most common hepatic disease in dogs. Copper accumulation is an important cause of chronic hepatitis in dogs; however, the etiology in most dogs cannot be determined. Clinical signs of chronic hepatitis are often non-specific; therefore, this disease is frequently diagnosed in an advanced stage that makes successful intervention less likely. Early diagnosis of chronic hepatitis in dogs would thus be beneficial. The identification of proteins that are differentially expressed in dogs with chronic hepatitis could contribute to the development of novel diagnostic markers for this disease and provide insight into its pathogenesis...
2018: PloS One
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