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https://www.readbyqxmd.com/read/30315711/adipose-derived-mesenchymal-stem-cells-alleviated-osteoarthritis-and-chondrocyte-apoptosis-through-autophagy-inducing
#1
Jun Zhou, Yu Wang, Yiming Liu, Hanjiang Zeng, Hanshi Xu, Fan Lian
OBJECTIVE: We aim to explore the effect of adipose derived mesenchymal stem cells (ADMSCs) on a knee osteoarthritis rat model and analyze how ADMSCs affect chondrocyte apoptosis. MATERIALS AND METHODS: A surgically induced rat knee osteoarthritis (OA) model was constructed. ADMSCs were engrafted into the right knee cavity. Hematoxylin and eosin (H&E), Masson, and Safranin O were used to compare the histopathology of synovial membrane and cartilage. Immunohistochemical (IHC) was used to measure MMP-13, Collagen 2 (Col-2), Caspase-3 (Cas-3), PARP, p62, LC3b, DDR-2, FGFR-1, Wnt, P-AKT/AKT, p-CAMKII/CAMKII, and p-Smad1/Smad1 expression in the articular cartilage...
October 13, 2018: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/30314700/alisol-b-23-acetate-a-tetracyclic-triterpenoid-isolated-from-alisma-orientale-induces-apoptosis-in-human-lung-cancer-cells-via-the-mitochondrial-pathway
#2
Jinxia Wang, Haizhen Li, Xiaoning Wang, Tao Shen, Shuqi Wang, Dongmei Ren
Alisol B-23-acetate (AB23A), a tetracyclic triterpenoid isolated from the rhizome of Alisma orientale, has been reported to exert anti-proliferative activities in human colon, ovarian and gastric cancer cells. However, the anti-cancer effect of this compound on human lung cancer cells has not yet been thoroughly elucidated. In the present study, we investigated the effects of AB23A on the cell viability and apoptosis in human lung cancer A549 and NCI-H292 cells. The results indicated that AB23A inhibited the growth of A549 and NCI-H292 cells in dose- and time-dependent manner, however, there was only weak cytotoxicity on normal bronchial epithelial cells...
October 9, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/30314496/mir-218-produces-anti-tumor-effects-on-cervical-cancer-cells-in-vitro
#3
Li Zhu, Huaidong Tu, Yanmei Liang, Dihong Tang
BACKGROUND: As indoleamine-2,3-dioxygenase 1 (IDO1) is critical in tumor immune escape, we determined to study the regulatory mechanism of miR-218 on IDO1 in cervical cancer. METHODS: Real-time PCR (RT-qPCR) was carried out to measure the expression of miR-218. RT-qPCR and Western blot were performed to detect the expression of IDO1 in cervical cancer. Dual-luciferase reporter assay was used to determine the binding of miR-218 on the IDO1 3'UTR. Cell viability, apoptosis, and related factors were determined using cell counting kit-8 (CCK-8), Annexin-V/PI (propidium) assay, enzyme-linked immunosorbnent assay (ELISA), RT-qPCR, and Western blot assays after miR-218 mimics has been transfected to HeLa cervical cancer cells...
October 12, 2018: World Journal of Surgical Oncology
https://www.readbyqxmd.com/read/30314336/synthesis-and-biological-evaluation-of-novel-dehydroabietic-acid-oxazolidinone-hybrids-for-antitumor-properties
#4
Xiu Wang, Fu-Hua Pang, Lin Huang, Xin-Ping Yang, Xian-Li Ma, Cai-Na Jiang, Fang-Yao Li, Fu-Hou Lei
Novel representatives of the important group of biologically-active, dehydroabietic acid-bearing oxazolidinone moiety were synthesized to explore more efficacious and less toxic antitumor agents. Structures of all the newly target molecules were confirmed by IR, ¹H-NMR, 13 C-NMR, and HR-MS. The inhibitory activities of these compounds against different human cancer cell lines (MGC-803, CNE-2, SK-OV-3, NCI-H460) and human normal liver cell line LO2 were evaluated and compared with the commercial anticancer drug cisplatin, using standard MTT (methyl thiazolytetrazolium) assay in vitro...
October 11, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/30312390/astaxanthin-enhances-the-longevity-of-saccharomyces-cerevisiae-by-decreasing-oxidative-stress-and-apoptosis
#5
Sudharshan Sj, Bhavana Veerabhadrappa, Subasri Subramaniyan, Madhu Dyavaiah
The budding yeast, Saccharomyces cerevisiae, is an efficient model for studying oxidative stress, programmed cell death, and aging. The present study was carried out to investigate antioxidant, the anti-apoptotic and antiaging activity of a natural compound, astaxanthin, in S. cerevisiae model. The survivability of yeast antioxidant deficient strains (sod1Δ, sod2Δ, cta1Δ, ctt1Δ and tsa1Δ) increased by 20-40% when cells were pre-treated with astaxanthin, compared to hydrogen peroxide alone, as demonstrated in spot and colony forming unit assays...
October 12, 2018: FEMS Yeast Research
https://www.readbyqxmd.com/read/30311181/sodium-valproate-ameliorates-neuronal-apoptosis-in-a-kainic-acid-model-of-epilepsy-via-enhancing-pkc-dependent-gaba-a-r-%C3%AE-2-serine-327-phosphorylation
#6
Qin Li, Qiu-Qi Li, Ji-Ning Jia, Shan Cao, Zhi-Bin Wang, Xu Wang, Chao Luo, Hong-Hao Zhou, Zhao-Qian Liu, Xiao-Yuan Mao
GABA is a dominant inhibitory neurotransmitter in the brain and A type GABA receptor (GABAA R) phosphorylation is critical for GABA-mediated inhibitory effect. However, its role in the neuroprotective effect of sodium valproate (VPA), a prevalent drug for treating patients with epilepsy, remains elusive. The present study was conducted to explore the role of GABAA R phosphorylation in the neuroprotection of VPA against a kainic acid-induced epileptic rat model and the potential molecular mechanisms. Neuronal apoptosis was evaluated by TUNEL assay, PI/Annexin V double staining, caspase-3 activity detection and Bax and Bcl-2 proteins expression via Western blot analysis...
October 11, 2018: Neurochemical Research
https://www.readbyqxmd.com/read/30311121/comparison-of-hypocrellin-b-mediated-sonodynamic-responsiveness-between-sensitive-and-multidrug-resistant-human-gastric-cancer-cell-lines
#7
Yichen Liu, Hong Bai, Haiping Wang, Xiaobing Wang, Quanhong Liu, Kun Zhang, Pan Wang
OBJECTIVES: The aim of this study was to compare the different responses to hypocrellin B (HB)-mediated sonodynamic treatment between human gastric adenocarcinoma cell line SGC7901 and SGC7901/ADR. METHODS: Tumor cells in culture dishes (35-mm diameter) were exposed to planar ultrasound at an intensity of 0.5 W/cm2 for 60 s combined with/without 2.5 μM HB. Cell viability was determined by MTT and Guava ViaCount assay. Production of reactive oxygen species (ROS) and destabilization of the mitochondrial membrane potential were assessed by flow cytometry...
October 11, 2018: Journal of Medical Ultrasonics
https://www.readbyqxmd.com/read/30311029/cox6b1-relieves-hypoxia-reoxygenation-injury-of-neonatal-rat-cardiomyocytes-by-regulating-mitochondrial-function
#8
Wei Zhang, Yu Wang, Junzhe Wan, Pengbo Zhang, Fei Pei
OBJECTIVE: Mitochondrial dysfunction plays a pivotal role in various pathophysiological processes of heart. Cytochrome oxidase subunit 6B1 (COX6B1) is a subunit of cytochrome oxidase. METHODS: Cardiomyocytes were isolated from neonatal SD rats (within 24 h of birth) by repeating digestion of collagenase and trypsin. COX6B1 over-expression and hypoxia/reoxygenation was conducted on neonatal rat cardiomyocytes. Cell viability, apoptosis rates, mitochondria membrane potential and mitochondrial permeabilization transition pores (mPTPs) were then determined respectively by Cell performing Counting Kit-8 (CCK-8), Annexin-V/PI assay, JC-1 assay, mPTP assay...
October 11, 2018: Biotechnology Letters
https://www.readbyqxmd.com/read/30308485/azd5153-inhibits-prostate-cancer-cell-growth-in-vitro-and-in-vivo
#9
Gang Shen, Jianchun Chen, Yongqiang Zhou, Zhenfan Wang, Zheng Ma, Chen Xu, Minjun Jiang
BACKGROUNDS/AIMS: Bromodomain-containing protein 4 (BRD4) overexpression participates in prostate cancer progression by enhancing the transcriptional activity and expression of several key oncogenes. AZD5153 is a novel BRD4 inhibitor. METHODS: Prostate cancer cells were treated with AZD5153. Cell survival was tested by MTT assay and clonogenicity assay. Cell proliferation was tested by [H3] DNA incorporation assay. Cell apoptosis was tested by caspase-3/-9 activity assay, Histone DNA ELISA assay, Annexin V FACS assay and TUNEL staining assay...
October 11, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/30304747/antiphospholipid-antibodies-in-a-general-obstetric-population-clinical-impact-on-pregnancy-outcome-and-relationship-with-the-m2-haplotype-in-the-annexin-a5-anxa5-gene
#10
Michela Villani, Donatella Colaizzo, Pasquale Martinelli, Filomena Cappucci, Lucia Fischetti, Giuseppe M Maruotti, Mariano Intrieri, Luigi Greco, Elena Chinni, Giovanni L Tiscia, Maurizio Margaglione, Elvira Grandone
Antiphospholipid (aPL) antibodies are recognised risk factors for adverse obstetric outcomes. Recently, carriers of the M2 haplotype in the Annexin A5 gene have been shown to have a higher susceptibility to develop aPL antibodies. In a general obstetric population, we prospectively evaluated the possible relationship between: (1) aPL antibodies and M2 haplotype; and (2) aPL antibodies and/or M2 haplotype and obstetric outcomes. From a cohort of 3,097 consecutive pregnant women, 1,286 samples were analysed for the presence of both anti-cardiolipin and anti-human β2-glycoprotein I antibodies; samples with available DNA ( n  = 606) were also investigated for the M2 haplotype...
October 10, 2018: Hämostaseologie
https://www.readbyqxmd.com/read/30302601/dna-binding-cleavage-and-cytotoxicity-studies-of-three-mononuclear-cu-ii-chloro-complexes-containing-n-s-donor-schiff-base-ligands
#11
Sidhali U Parsekar, Joseph Fernandes, Arnab Banerjee, Om Prakash Chouhan, Sumit Biswas, Manohar Singh, Durga P Mishra, Manjuri Kumar
We report the biological activity of three Cu(II) complexes [Cu(pabt)Cl] (1), [Cu(pma)Cl] (2), and [Cu(pdta)Cl]Cl (3) (pabt = N-(2-mercaptophenyl)-2'-pyridylmethylenimine, pma = N-(2-pyridylmethyl)-2-mercaptoaniline, pdta = 2,2'-di(pyridyl-2-methyleneimine)diphenyl disulfide). 1-3 display four-line EPR multiplet in solution at RT suggesting that these are mononuclear. DNA-binding studies using spectrophotometric titration of these complexes with calf thymus DNA showed binding through intercalation mode which was found to be consistent with the observation of increased viscosity of DNA and quenching of fluorescence of ethidium bromide bound DNA in the presence of these complexes...
October 9, 2018: Journal of Biological Inorganic Chemistry: JBIC
https://www.readbyqxmd.com/read/30300910/monocytes-of-different-subsets-in-complexes-with-platelets-in-patients-with-myocardial-infarction
#12
Marina Loguinova, Natalia Pinegina, Valeria Kogan, Murad Vagida, Anush Arakelyan, Alexander Shpektor, Leonid Margolis, Elena Vasilieva
Acute myocardial infarction (AMI) is associated with activation of various cells, including platelets that form monocyte-platelet complexes (MPCs). Here, we analysed MPC in vivo and in vitro and investigated the abilities of different monocyte subclasses to form MPC, the characteristics of the cells involved in MPC formation and MPC changes in AMI. We identified MPC by co-staining for platelet antigen CD41a and monocyte antigens CD14 and CD16. Platelet activation was evaluated from expression of phosphatidylserine as revealed by annexin V...
October 9, 2018: Thrombosis and Haemostasis
https://www.readbyqxmd.com/read/30298827/design-synthesis-and-screening-of-5-aryl-3-2-pyrrolyl-thiophenyl-1-2-4-oxadiazoles-as-potential-antitumor-molecules-on-breast-cancer-mcf-7-cell-line
#13
Mohammed K Abd El Hameid
The work reported the design and cytotoxic screening of synthetic small molecules: carbonitriles 3a-c, carboximidamides 4a-c, and oxadiazoles 5-19 as antitumor molecules. Molecules 4c, 9, 12, and 14 show promising cytotoxicity profiles against two cell lines higher than prodigiosin (PG). The results of topoisomerase enzyme inhibition assay show that carboximidamide 4c and oxadiazole 14 display potent inhibitory activity in nano-molar concentration higher than PG. In addition, carboximidamide 4c and oxadiazoles 9, 12, and 14 exhibit antiproliferative activities over MCF-7 cells by cell cycle arrest at G1  phase and apoptosis inducing activity by increasing cell population percentages at pre G1  and G2 /M phases as shown by DNA-flow cytometry assay and annexin V analysis...
October 5, 2018: Chemical & Pharmaceutical Bulletin
https://www.readbyqxmd.com/read/30293105/chitosan-nanoparticles-from-artemia-salina-inhibit-progression-of-hepatocellular-carcinoma-in-vitro-and-in-vivo
#14
Mai M Elkeiy, Abeer A Khamis, Mona M El-Gamal, Maha M Abo Gazia, Zeinb A Zalat, Mohammed A El-Magd
This study was conducted to evaluate the effect of chitosan nanoparticles (CNPs) isolated from Artemia salina against hepatocellular carcinoma (HCC) both in vitro (HepG2) and in vivo (diethylnitrosamine-induced HCC in rats) and to investigate the involved underlying mechanisms. Administration of CNPs decreased HCC progression as evidenced by (1) induced HepG2 cell death as detected by MTT assay; (2) induced necrosis as indicated by acridine orange/propidium iodide (AO/PI) red staining, annexin V/7-AAD positive staining (detected by flow cytometry), and upregulated expression of necrosis markers (PARP1 and its downstream target, RIP1 genes), but no effect on apoptosis as revealed by insignificant changes in caspase 3 activity and mRNA levels of Bax and AIF; (3) increased intracellular ROS and decreased mitochondrial membrane potential in HepG2; (4) decreased liver relative weight, serum levels of liver enzymes (ALT, AST, and ALP), total bilirubin, and cancer markers (AFP and GGT), number and area of GST-P positive tumor nodules; and (5) reduced oxidative stress (decrease in MDA levels) and increased activities of SOD, CAT, and GPx enzymes in rat liver...
October 6, 2018: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/30288110/ethylacetate-extract-from-tetrastigma-hemsleyanum-inhibits-proliferation-and-induces-apoptosis-in-hepg2-and-smmc-7721-cells
#15
Shipin Chen, Meixiu Luo, Liang Ma, Wenjun Lin
Purpose: This study aimed to investigate the effect of ethylacetate extract from Tetrastigma hemsleyanum (EET) on the proliferation and apoptosis of HepG2 and SMMC-7721 cells and determine the underlying mechanisms. Materials and methods: HepG2 and SMMC-7721 cells were cultured in vitro until the exponential growth phase and then treated with different concentrations of EET for 24 h. We performed a colony forming assay to determine colony forming ability, CCK8 assay to detect cell proliferation, Annexin V-FITC/PI double staining to analyze cell apoptosis, and Western blot to measure the protein expression of Caspase-3, Bcl-2, and Bax...
2018: Cancer Management and Research
https://www.readbyqxmd.com/read/30287338/ochratoxin-a-induces-cytoprotective-autophagy-via-blocking-akt-mtor-signaling-pathway-in-pk-15-cells
#16
Gang Qian, Dandan Liu, Lili Hou, Mohammed Hamid, Xingxiang Chen, Fang Gan, Suquan Song, Kehe Huang
Ochratoxin A (OTA) could cause a variety of toxicological effects especially nephrotoxicity in animals and humans. Autophagy is a highly conserved metabolic process that plays an important role in the maintenance of cellular homeostasis under stress. However, the role of autophagy in OTA-induced nephrotoxicity is unknown. In the present study, we demonstrated that OTA treatment at 2.0-8.0 μM could increase cytotoxicity of PK-15 cells by inducing apoptosis as shown by the increased Annexin V/PI staining, increased caspase-3 and PARP cleavage and increased apoptotic nuclei...
October 1, 2018: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/30286469/microrna-29a-inhibits-growth-migration-and-invasion-of-melanoma-a375-cells-in-vitro-by-directly-targeting-bmi1
#17
Ying Xiong, Liqian Liu, Ying Qiu, Lili Liu
BACKGROUND/AIMS: Melanoma is one of the most aggressive malignant tumors, with increasing incidence, poor prognosis, and lack of any effective targeted therapies. Abnormal expression of miR-29a has been found in several types of cancers, including melanoma. In this study, experiments were performed to investigate the role of miR-29a in melanoma, and the molecular mechanism by which miR-29a represses melanoma. METHODS: miR-29 and Bmi1 expression was examined by quantitative real-time polymerase chain reaction (qRT-PCR)...
October 4, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/30286327/expression-of-the-annexin-a1-and-its-correlation-with-matrix-metalloproteinases-and-the-receptor-for-formylated-peptide-2-in-diffuse-astrocytic-tumors
#18
Maryam Buainain Tadei, Matheus Vicente Mayorquim, Camila Brambilla de Souza, Sara de Souza Costa, Lucas Possebon, Helena Ribeiro Souza, Melina Mizusaki Iyomasa-Pilon, Mairto Roberis Geromel, Ana Paula Girol
Astrocytomas represent the majority of cerebral gliomas. Studies show that the anti-inflammatory protein Annexin-A1 (ANXA1) is associated with the tumor invasion process and that its actions can be mediated by the receptor for formylated peptides (FPR). Therefore, we evaluated the expression of ANXA1, the receptor FPR2 and matrix metalloproteinases 2 and 9 (MMP-2 and MMP-9) in brain astrocytomas. Detection of proteins was performed in sections of diffuse astrocytomas (grade II), anaplastic astrocytomas (grade III) and glioblastomas (GBM, grade IV) and quantifications were made by densitometry...
September 3, 2018: Annals of Diagnostic Pathology
https://www.readbyqxmd.com/read/30282879/in-vitro-and-in-vivo-detection-of-drug-induced-apoptosis-using-annexin-v-conjugated-ultrasmall-superparamagnetic-iron-oxide-uspio-a-pilot-study
#19
Akihiro Nishie, Osamu Togao, Chihiro Tamura, Mayumi Yamato, Kazuhiro Ichikawa, Satoshi Nohara, Yoshio Ito, Naoki Kato, Satoshi Yoshise, Hiroshi Honda
PURPOSE: To investigate the binding potential of newly developed Annexin V-conjugated ultrasmall superparamagnetic iron oxide (V-USPIO) for detection of drug-induced apoptosis in vitro and in vivo. METHODS: Apoptotic cells induced by camptothecin were incubated with or without Annexin V-USPIO at a concentration of 0.089 mmol Fe/L in vitro. T2 values of the two cell suspensions were measured by 0.47T nuclear magnetic resonance (NMR) spectrometer. Tumor-bearing mice were subjected to 1...
October 2, 2018: Magnetic Resonance in Medical Sciences: MRMS
https://www.readbyqxmd.com/read/30277307/perfluorooctanoic-acid-disrupts-gap-junction-intercellular-communication-and-induces-reactive-oxygen-species-formation-and-apoptosis-in-mouse-ovaries
#20
Patricia López-Arellano, Keila López-Arellano, Jaquelinne Luna, Diana Flores, Javier Jiménez-Salazar, Graciela Gavia, Mario Teteltitla, Juan José Rodríguez, Alejandro Domínguez, Eduardo Casas, Ivan Bahena, Miguel Betancourt, Cristina González, Yvonne Ducolomb, Edmundo Bonilla
Perfluorooctanoic acid (PFOA) is a member of the perfluoroalkyl acid family of compounds. Due to the presence of strong carbon-fluorine bonds, it is practically nonbiodegradable and highly persistent in the environment. PFOA has been detected in the follicular fluid of women, and positively associated with reduced fecundability and infertility. However, there are no reports concerning the experimental evaluation of PFOA on oocyte toxicity in mammals. The aim of the present study was to determine if PFOA is able to induce oxidative stress in fetal ovaries and cause apoptosis in oocytes in vitro...
October 2, 2018: Environmental Toxicology
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