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https://www.readbyqxmd.com/read/30119256/synergy-of-theophylline-reduces-necrotic-effect-of-berberine-induces-cell-cycle-arrest-and-parp-hmgb1-bcl-2-family-mediated-apoptosis-in-mda-mb-231-breast-cancer-cells
#1
Fatemeh Hashemi-Niasari, Azra Rabbani-Chadegani, Mahdieh Razmi, Soudabeh Fallah
Berberine, is a plant alkaloid, proved to have anticancer effect on various cancers. Theophylline (TH), a natural product, is widely used in the treatment of respiratory difficulties. The present study designed to elucidate the effects of theophylline and berberine combination on breast cancer cells cytotoxicity, gene expression and cell cycle. MTT assay revealed that berberine inhibited MDA-MB-231 breast cancer cells viability in a time and dose dependent manner (IC50 of 100 μM) but theophylline had no considerably effect on the cells...
July 11, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/30117517/a-glucogalactomannan-isolated-from-agaricus-bisporus-induces-apoptosis-in-macrophages-through-the-jnk-bim-caspase-3-pathway
#2
Xiaotong Zhao, Leilei Fang, Danting Liu, Changjiangsheng Lai, Yanqing Zhang, Aimin Zhou, Junbo Xie
Agaricus bisporus is one of the most important edible and medicinal mushrooms in the world. It has been well known that Agaricus bisporus has an immunoregulatory role, but its active ingredients have not been completely identified. In this study, a glucogalactomannan named TJ3 was isolated and purified from Agaricus bisporus. TJ3 (827 kDa) is composed of mannose, galactose, glucose and xylose in the ratio 28.26 : 27.82 : 20.88 : 9.87 mainly joined by β-linkages. Functional analysis of TJ3 revealed that it effectively induced apoptosis in RAW 264...
August 17, 2018: Food & Function
https://www.readbyqxmd.com/read/30116240/differential-mechanisms-of-septic-human-pulmonary-microvascular-endothelial-cell-barrier-dysfunction-depending-on-the-presence-of-neutrophils
#3
Lefeng Wang, Sanjay Mehta, Yousuf Ahmed, Shelby Wallace, M Cynthia Pape, Sean E Gill
Sepsis is characterized by injury of pulmonary microvascular endothelial cells (PMVEC) leading to barrier dysfunction. Multiple mechanisms promote septic PMVEC barrier dysfunction, including interaction with circulating leukocytes and PMVEC apoptotic death. Our previous work demonstrated a strong correlation between septic neutrophil (PMN)-dependent PMVEC apoptosis and pulmonary microvascular albumin leak in septic mice in vivo ; however, this remains uncertain in human PMVEC. Thus, we hypothesize that human PMVEC apoptosis is required for loss of PMVEC barrier function under septic conditions in vitro ...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/30116143/endothelial-microvesicles-and-soluble-markers-of-endothelial-injury-in-critically-ill-newborns
#4
Veronika Vítková, Martin Pánek, Petr Janec, Michaela Šibíková, Václav Vobruba, Martin Haluzík, Jan Živný, Jan Janota
Neonatal systemic inflammatory response and multiple organ dysfunction syndrome are the main postnatal insults influencing mortality and morbidity. Critically ill newborns with high predicted mortality are supported by extracorporeal membrane oxygenation (ECMO). Biomarkers of inflammatory response and endothelial injury can be used for early diagnosis and treatment of critical neonatal situations. The aim of our study was to explore plasma proteins and endothelial microvesicles as markers of inflammation and endothelial activation in newborns on ECMO and to compare them with healthy neonates...
2018: Mediators of Inflammation
https://www.readbyqxmd.com/read/30112141/apoptosis-activity-of-the-mouse-macrophage-cell-line-j774a-1-infected-with-a-recombinant-bcg-consisting-the-c-terminus-of-merozoite-surface-protein-1-of-plasmodium-falciparum
#5
Anis Fadhilah Zulkipli, Nor Munirah Zakaria, Mohamed Hussein Abdikarim, Maryam Azlan, Nurulasma Abdullah, Norazmi Mohd Nor, Rapeah Suppian
Macrophage apoptosis exerts an efficient mechanism in controlling intracellular infection during innate immune response against various pathogens including malaria parasites. This study was carried out to determine the apoptosis activity in mouse macrophage cell line J774A.1 infected with a Mycobacterium bovis bacille Calmette-Guerin (BCG) clone and a recombinant BCG clone expressing the C-terminus of merozoite surface protein-1 (BCG-MSP1C) of Plasmodium falciparum for 48 h. In this study, a parent BCG cells was used as a control...
July 2018: Tropical Life Sciences Research
https://www.readbyqxmd.com/read/30112029/echinacoside-suppresses-dexamethasone-induced-growth-inhibition-and-apoptosis-in-osteoblastic-mc3t3-e1-cells
#6
Sibo Li, Haitao Jiang, Xiaohua Gu
Glucocorticoids (GCs) are widely used to treat chronic diseases. Prolonged and/or overdose administration of GCs has many side-effects to human health including GC-induced osteoporosis (GIOP). In this investigation, the objective was to assess the influence that echinacoside (ECH) exerts upon dexamethasone-treated murine osteoblastic MC3T3-E1 cells. We found that ECH (5, 10, 20 and 40 mg/l) inhibited dexamethasone (1,000 nM)-suppressed cell viability as demonstrated by Cell Counting Kit-8 (CCK-8) assay. The dose of 10 mg/l was selected for the following experiments because this dose had a better effect than the dose of 5 mg/l, and the doses >10 mg/l had a similar effect as this dose...
August 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/30111667/design-synthesis-and-screening-of-4-6-diaryl-pyridine-and-pyrimidine-derivatives-as-potential-cytotoxic-molecules
#7
Mohammed K Abd Elhameid, Noha Ryad, Al-Shorbagy My, Manal R Mohammed, Mohammed M Ismail, Salwa El Meligie
A new series of pyridine and pyrimidine derivatives is designed and synthesized as potential antitumor molecules. The tested compounds show promising in vitro cytotoxic activity against HL-60 cell line as eight compounds: 4, 6, 11, 13, 14, 15, 18 and 21 exhibit potent cytotoxic activity in sub-micromolar concentration higher than the combretastatin A4 (CA-4). Compound 21 shows a cytotoxic activity 5- fold more potent than CA-4 on HL-60 cells. DNA- Flow cytometry cell cycle analysis and annexin-V assay on HL-60 cells show that compounds 4, 18 and 21 exhibit potent cell growth inhibition, cell cycle arrest at G2 /M phase and pro-apoptotic inducing activities...
August 14, 2018: Chemical & Pharmaceutical Bulletin
https://www.readbyqxmd.com/read/30109997/-mutations-of-g38r-and-d40g-cause-amyotrophic-lateral-sclerosis-by-reducing-annexin-a11-protein-stability
#8
Di Liao, Qiao Liao, Cao Huang, Fangfang Bi
To explore the role of the mutations G38R and D40G of Annexin A11 (ANXA11) in the onset of amyotrophic lateral sclerosis (ALS).
 Methods: The plasmids expressing ANXA11 wild type protein, ANXA11 G38R protein and ANXA11 D40G protein were constructed, respectively. The recombinant plasmids were then transfected into HEK293 cells respectively followed by cycloheximide (CHX) treatment for 0, 2, 4 and 8 h. The protein expressions of ANXA11 wild type, ANXA11 G38R and ANXA11 D40G mutations were determined by Western blot...
June 28, 2018: Zhong Nan da Xue Xue Bao. Yi Xue Ban, Journal of Central South University. Medical Sciences
https://www.readbyqxmd.com/read/30108921/new-3-1-h-benzo-d-imidazol-2-yl-3-3-h-imidazo-4-5-b-pyridin-2-yl-1-h-indol-5-yl-3-4-5-trimethoxyphenyl-methanone-conjugates-as-tubulin-polymerization-inhibitors
#9
Kishore Mullagiri, V Lakshma Nayak, Satish Sunkari, Geeta Sai Mani, Sravanthi Devi Guggilapu, Burri Nagaraju, Abdullah Alarifi, Ahmed Kamal
A series of new (3-(1 H -benzo[ d ]imidazol-2-yl))/(3-(3 H -imidazo[4,5- b ]pyridin-2-yl))-(1 H -indol-5-yl)(3,4,5-trimethoxyphenyl)methanone conjugates 4-6(a-i) were synthesized and evaluated for their antiproliferative activity on selected human cancer cell lines such as prostate (DU-145), lung (A549), cervical (HeLa) and breast (MCF-7). Most of these conjugates showed considerable cytotoxicity with IC50 values ranging from 0.54 to 31.86 μM. Among them, compounds 5g and 6f showed significant activity against human prostate cancer cell line DU-145 with IC50 values of 0...
February 1, 2018: MedChemComm
https://www.readbyqxmd.com/read/30108431/irisin-protects-macrophages-from-oxidized-low-density-lipoprotein-induced-apoptosis-by-inhibiting-the-endoplasmic-reticulum-stress-pathway
#10
Guanlin Zheng, Haizhen Li, Tie Zhang, Libo Yang, Shutong Yao, Shihong Chen, Maochuan Zheng, Qin Zhao, Hua Tian
Irisin is a newly discovered myokine which can relieve metabolic disorders and resist atherosclerosis. The effects of irisin on ox-LDL-induced macrophage apoptosis and endoplasmic reticulum stress-related pathways were observed in vitro . RAW264.7 macrophages were cultured in vitro and pretreated with irisin at 20, 40 and 80 ng/ml for 30 min, followed by culture with 100 mg/L ox-LDL and 5 mg/L tunicamycin (TM) for 12 h. The cell viability and apoptosis were detected by MTT assay and annexin V-FITC double staining...
July 2018: Saudi Journal of Biological Sciences
https://www.readbyqxmd.com/read/30106753/a-novel-hedgehog-inhibitor-for-the-treatment-of-hematological-malignancies
#11
Peng Lin, Yuanming He, Guodong Chen, Haikuo Ma, Jiyue Zheng, Zubin Zhang, Biyin Cao, Hongjian Zhang, Xiaohu Zhang, Xinliang Mao
The hedgehog-smoothened (HH/SMO) pathway has been proposed as a potential therapeutic target for hematological malignancies. Our previous studies designed a series of HH inhibitors with novel scaffolds distinctive from vismodegib, the first Food and Drug Administration-approved HH inhibitor for the treatment of basal-cell carcinoma and medulloblastoma. In the present study, we evaluated these HH inhibitors against blood cancers and found that HH78 displayed potent activity in suppressing the HH signaling pathway...
August 13, 2018: Anti-cancer Drugs
https://www.readbyqxmd.com/read/30106542/the-effects-of-male-age-on-sperm-dna-damage-an-evaluation-of-2-178-semen-samples
#12
Claudia G Petersen, Ana L Mauri, Laura D Vagnini, Adriana Renzi, Bruna Petersen, Mariana Mattila, Vanessa Comar, Juliana Ricci, Felipe Dieamant, Joao Batista A Oliveira, Ricardo L R Baruffi, Jose G Franco
OBJECTIVE: This study aimed to evaluate the effects of male age on sperm DNA damage. METHODS: This cross-sectional study included semen samples collected from 2,178 men seen at an infertility clinic. For DNA integrity analysis, the proportions of spermatozoa showing DNA fragmentation (TUNEL assay), abnormal chromatin packaging/underprotamination (chromomycin A3 ), abnormal mitochondrial membrane potential (MMP/MitoTracker Green), and apoptosis (annexin V) were recorded...
August 14, 2018: JBRA Assisted Reproduction
https://www.readbyqxmd.com/read/30106156/immune-thrombocytopenia-induces-autophagy-and-suppresses-apoptosis-in-megakaryocytes
#13
Zhanshu Liu, Tonghua Mei
Immune thrombocytopenia (ITP) is the main pathogenesis of excessive platelet destruction and abnormal megakaryocyte apoptosis, however, the mechanism underlying this abnormality in megakaryocytes remains to be elucidated. Since autophagy and apoptosis are closely interrelated, it can be speculated that the abnormal apoptosis of ITP megakaryocytes is associated with autophagy. To test this hypothesis, a total of 14 patients with ITP and 23 healthy controls were recruited. MEG‑01 cell line was cultured in vitro, and morphological changes were observed by light microscopy, apoptosis was evaluated by flow cytometric analysis of Annexin V‑FITC/propidium iodide staining and western blot analysis of B‑cell lymphoma (Bcl)‑2, Bcl‑associated X protein (Bax), Beclin‑1 and cleaved caspase 3...
August 9, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/30106144/a-natural-ent%C3%A2-kaurane-diterpenoid-induces-antiproliferation-in-ovarian-cancer-cells-via-erk1-2-regulation-and-inhibition-of-cellular-migration-and-invasion
#14
Jin Sun Lee, Myung Sun Lee, Eun Young Cha, Phuong Thien Thuong, Ji Young Sul, Jun Beom Park, Young Bok Ko
Ovarian cancer is one of the most common causes of female mortalities from gynecological tumors. An ent‑kaurane diterpenoid compound CRT1 (ent‑18‑acetoxy‑7β‑hydroxy kaur‑15‑oxo‑16‑ene), mainly isolated from the Vietnamese herb Croton tonkinesis has been used in folk medicine in Vietnam for cancer treatment. However, the effect of this compound on human ovarian cancer cells has not yet been reported. The objective of the present study was to determine the effect of CRT1 on the cell viability, apoptosis and metastasis of SKOV3 human ovarian cancer cells using a Cell Counting Kit‑8 assay, flow cytometric analysis of Annexin V‑fluorescein isothiocyanate/propidium iodide staining, western blot analysis, soft agar colony forming assay, wound healing assay and Matrigel invasion assay...
August 9, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/30104508/ursolic-acid-induced-apoptosis-via-regulation-of-the-pi3k-akt-and-mapk-signaling-pathways-in-huh-7-cells
#15
Kwong-Chiu Lee, Yao-Li Chen, Ping-Yi Lin, Wan-Ling Chuang
Ursolic acid (UA), is a kind of triterpene acid that exhibits wide biological properties. In this article, the effects of UA on apoptosis and the proliferation of human hepatoma Huh-7 cells were reported. The MTT results showed that cell viability of Huh-7 was reduced in a concentration and time-dependent effect. In addition, DAPI staining was used to detected condensation of chromatin in nucleus. Apoptotic cell population was examined using Annexin V/PI staining. The results showed that exposure to UA affected extrinsic and intrinsic pathways through, reduced expression of Bcl-2, Mcl-1, and TCTP; increased levels of the apoptotic proteins TNF-α, Fas, FADD, and Bax; and activation of cleaved caspase-3 and PARP...
August 13, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/30100745/euphornin-reduces-proliferation-of-human-cervical-adenocarcinoma-hela-cells-through-induction-of-apoptosis-and-g2-m-cell-cycle-arrest
#16
Xiao-Qiang Li, Yin-Liang Bai, De-Li Zhang, Hai-Sheng Jiao, Rong-Xia He
Background: The plant Euphorbia helioscopia L. has been used in traditional Chinese medicine for treating various disorders such as tuberculosis and edema. The aim of this study was to investigate the effect of euphornin, a bioactive compound isolated from E. helioscopia , on proliferation of human cervical adenocarcinoma HeLa cells by analyzing cell viability, rate of apoptosis, and cell cycle progression. Materials and methods: The sulforhodamine B assay was used to study the effect of euphornin on the proliferation of HeLa cells...
2018: OncoTargets and Therapy
https://www.readbyqxmd.com/read/30099272/dibenzoxanthenes-induce-apoptosis-and-autophagy-in-hela-cells-by-modeling-the-pi3k-akt-pathway
#17
Xiu-Zhen Wang, Zhi Jia, Hui-Hui Yang, Yun-Jun Liu
A new series of dibenzoxanthene derivatives 4a-4d (4a: 1-oxo-5-bromo-11-cyano-13c-methoxy-1,13c-dihydroxyl-dibenzo[a,kl]xanthene, 4b: 1-oxo-5-bromo-11-cyano-13c-ethoxy-1,13c-dihydroxyl-dibenzo[a,kl]xanthene, 4c: 1-oxo-5-bromo-11-cyano-13c-propoxy-1,13c-dihydroxyl-dibenzo[a,kl]xanthene and 4d: 1-oxo-5-bromo-11-cyano-13c-butoxy-1,13c-dihydroxyl-dibenzo[a,kl]xanthene) were synthesized and the molecular mechanisms of anti-cancer activities were investigated. These compounds showed excellent anti-tumor activity against A549, Eca-109, HeLa, HepG2 and SGC-7901 cell lines...
August 6, 2018: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/30099008/modulation-of-nitric-oxide-stimulated-soluble-guanylyl-cyclase-activity-by-cytoskeleton-associated-proteins-in-vascular-smooth-muscle
#18
Alexander Kollau, Bernd Gesslbauer, Michael Russwurm, Doris Koesling, Antonius C F Gorren, Astrid Schrammel, Bernd Mayer
Soluble guanylyl cyclase (sGC, EC 4.6.1.2) is a key enzyme in the regulation of vascular tone. In view of the therapeutic interest of the NO/cGMP pathway, drugs were developed that either increase the NO sensitivity of the enzyme or activate heme-free apo-sGC. However, modulation of sGC activity by endogenous agents is poorly understood. In the present study we show that the maximal activity of NO-stimulated purified sGC is significantly increased by cytosolic preparations of porcine coronary arteries. Purification of the active principle by several chromatographic steps resulted in a protein mixture consisting of 100, 70, and 40 kDa bands on SDS polyacrylamide gel electrophoresis...
August 9, 2018: Biochemical Pharmacology
https://www.readbyqxmd.com/read/30091159/-6-gingerol-induced-cell-cycle-arrest-reactive-oxygen-species-generation-and-disruption-of-mitochondrial-membrane-potential-are-associated-with-apoptosis-in-human-gastric-cancer-ags-cells
#19
Debjani P Mansingh, Sunanda O J, Veeresh Kumar Sali, Hannah R Vasanthi
Ginger (Zingiber officinale Roscoe), a monocotyledonous herb, is widely used as an herbal medicine owing to the phytoconstituents it possesses. In the current study, the quantity of [6]-gingerol, the major phenolic ketone, in the fresh ginger and dried ginger rhizome was found to be 6.11 µg/mg and 0.407 µg/mg. Furthermore, [6]-gingerol was assessed for its antiapoptotic effects in human gastric adenocarcinoma (AGS) cells evidenced by acridine orange/ethidium bromide staining technique and Annexin-V assay...
August 8, 2018: Journal of Biochemical and Molecular Toxicology
https://www.readbyqxmd.com/read/30090014/chitosan-inhibits-inflammation-and-adipogenesis-of-orbital-fibroblasts-in-graves-ophthalmopathy
#20
Haibo Xiong, Mingxing Wu, Hongmi Zou, Shaoqiu Jiang, Hong Yi, Taisong Yi, Qian Wang, Danning Liu, Yu Zhou, Changzheng Wei, Xiyuan Zhou
Purpose: The aim of this study was to investigate the roles of chitosan in inflammation and adipogenesis of primary cultured orbital fibroblasts in Graves ophthalmopathy (GO). Methods: Cell viability, apoptosis, and cell cycle were determined with the Cell Counting Kit-8 (CCK-8), the Annexin V-FITC/PI kit, and flow cytometry, respectively. Inflammation of orbital fibroblasts was stimulated by interleukin-1 beta (IL-1β). The levels of IL-6 and prostaglandin E-2 (PGE-2) were measured using an enzyme-linked immunosorbent assay (ELISA)...
2018: Molecular Vision
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