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Pseudevernia furfuracea

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https://www.readbyqxmd.com/read/29573381/effects-of-two-lichen-acids-isolated-from-pseudevernia-furfuracea-l-zopf-in-cultured-human-lymphocytes
#1
Bugrahan Emsen, Basak Togar, Hasan Turkez, Ali Aslan
The present study aims at assessing the efficacies of olivetoric acid (OA) and physodic acid (PA) isolated from Pseudevernia furfuracea (L.) Zopf (Parmeliaceae) in human lymphocytes (HLs) in vitro. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and lactate dehydrogenase assays were performed to establish cytotoxicity in HLs. Besides, oxidative stress and genotoxicity were monitored by estimating the changes of total oxidative stress (TOS) and 8-hydroxy-2'-deoxyguanosine (8-OH-dG) levels, respectively, in HLs...
July 26, 2018: Zeitschrift Für Naturforschung. C, A Journal of Biosciences
https://www.readbyqxmd.com/read/29421758/combining-spectroscopic-techniques-and-chemometrics-for-the-interpretation-of-lichen-biomonitoring-of-air-pollution
#2
P Malaspina, M Casale, C Malegori, M Hooshyari, M Di Carro, E Magi, P Giordani
A screening evaluation of lichen thalli, based on spectroscopic techniques coupled with chemometrics, is proposed as fast, simple and "green" method for the biomonitoring of air pollution. For two consecutive years, lichen thalli of Pseudevernia furfuracea were exposed for three months in selected sites of Liguria (NW-Italy) according to different levels and types of air pollution. At the end of the exposure period, transplanted thalli were analyzed by a set of monitoring techniques, including Front-Face Fluorescence Spectroscopy (FFFS), Near Infrared Spectroscopy (NIRS) and Plant Efficiency Analyser (PEA)...
May 2018: Chemosphere
https://www.readbyqxmd.com/read/29216469/background-element-content-of-the-lichen-pseudevernia-furfuracea-a-supra-national-state-of-art-implemented-by-novel-field-data-from-italy
#3
Elva Cecconi, Guido Incerti, Fiore Capozzi, Paola Adamo, Roberto Bargagli, Renato Benesperi, Fabio Candotto Carniel, Sergio Enrico Favero-Longo, Simonetta Giordano, Domenico Puntillo, Sonia Ravera, Valeria Spagnuolo, Mauro Tretiach
In biomonitoring, the knowledge of background element content (BEC) values is an essential pre-requisite for the correct assessment of pollution levels. Here, we estimated the BEC values of a highly performing biomonitor, the epiphytic lichen Pseudevernia furfuracea, by means of a careful review of literature data, integrated by an extensive field survey. Methodologically homogeneous element content datasets, reflecting different exposure conditions across European and extra-European countries, were compiled and comparatively analysed...
May 1, 2018: Science of the Total Environment
https://www.readbyqxmd.com/read/29097759/sequencing-genomes-from-mixed-dna-samples-evaluating-the-metagenome-skimming-approach-in-lichenized-fungi
#4
Anjuli Meiser, Jürgen Otte, Imke Schmitt, Francesco Dal Grande
The metagenome skimming approach, i.e. low coverage shotgun sequencing of multi-species assemblages and subsequent reconstruction of individual genomes, is increasingly used for in-depth genomic characterization of ecological communities. This approach is a promising tool for reconstructing genomes of facultative symbionts, such as lichen-forming fungi, from metagenomic reads. However, no study has so far tested accuracy and completeness of assemblies based on metagenomic sequences compared to assemblies based on pure culture strains of lichenized fungi...
November 2, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28426984/biomagnetic-monitoring-and-element-content-of-lichen-transplants-in-a-mixed-land-use-area-of-ne-italy
#5
Danijela Kodnik, Aldo Winkler, Fabio Candotto Carniel, Mauro Tretiach
The aim of this study was to verify whether it is possible to discriminate between the different pollution sources present in a mixed land use area of NE Italy on the basis of the magnetic properties and the element content of lichen transplants. Thalli of Pseudevernia furfuracea were collected in a pristine area of the South-Eastern Alps and exposed for 2months in 40 sites located at the knots of a 700m step grid covering ca. 40km2 of a mosaic of agricultural, forested, industrial and urban areas. In this way, the samples could be analyzed after a defined period of time, and compared to pre-exposure conditions...
October 1, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28308806/sulfite-action-on-ribulosediphosphate-carboxylase-in-the-lichen-pseudevernia-furfuracea
#6
Irmgard Ziegler
Ribulosediphosphate carboxylase can be extracted from Pseudevernia furfuracea, either by Triton X-100 or by pretreatment with liquid nitrogen. With respect to HCO 3(-) the K m is 33 mM. The enzyme is competitively inhibited by sulfite, as is the case with spinach chloroplasts. The K i value (15.5-18.5 mM sulfite) indicates that in lichens the enzyme is not more sensitive than that in spinach. Thus the extreme sensitivity of lichens to SO2 is not based on low tolerance at the enzymatic level, but is due to a low degree of avoidance...
March 1977: Oecologia
https://www.readbyqxmd.com/read/28132191/infraspecific-variability-in-baseline-element-composition-of-the-epiphytic-lichen-pseudevernia-furfuracea-in-remote-areas-implications-for-biomonitoring-of-air-pollution
#7
Guido Incerti, Elva Cecconi, Fiore Capozzi, Paola Adamo, Roberto Bargagli, Renato Benesperi, Fabio Candotto Carniel, Fabiana Cristofolini, Simonetta Giordano, Domenico Puntillo, Valeria Spagnuolo, Mauro Tretiach
The epiphytic lichen Pseudevernia furfuracea is widely used as biomonitor of airborne trace elements and other contaminants and consists of two taxonomic varieties (var. furfuracea and var. ceratea). Here, we assessed the occurrence of inter-varietal differences in the elemental composition of paired samples of var. furfuracea and var. ceratea collected in 20 remote sites of Italian mountains. The concentration of 40 elements was measured by inductively coupled plasma mass spectroscopy, after digestion with HNO3 and aqua regia...
March 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/27610744/biomonitoring-of-heavy-metals-contamination-by-mosses-and-lichens-around-slovinky-tailing-pond-slovakia
#8
Lenka Demková, Lenka Bobul'ská, Július Árvay, Tomáš Jezný, Ladislav Ducsay
Three moss (Pleurozium spp., Polytrichum spp., and Rhytidiadelphus spp.) and two lichen (Hypogymnia physodes and Pseudevernia furfuracea) taxons covered in the bags were used to monitor air quality. Bags were exposed at the different distances from the tailing pond because of insufficient security and source of heavy metal pollution. Moss/lichen bags were exposed for six weeks at 0-, 50-, 100-, 150- and 200-m distances from Slovinky tailing pond, in the main wind direction (down the valley). Accumulation ability of heavy metals expressed by relative accumulation factor (RAF) increases in the order of Polytrichum spp...
January 2, 2017: Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering
https://www.readbyqxmd.com/read/27494030/innovative-approaches-using-lichen-enriched-media-to-improve-isolation-and-culturability-of-lichen-associated-bacteria
#9
Elena G Biosca, Raquel Flores, Ricardo D Santander, José Luis Díez-Gil, Eva Barreno
Lichens, self-supporting mutualistic associations between a fungal partner and one or more photosynthetic partners, also harbor non-photosynthetic bacteria. The diversity and contribution of these bacteria to the functioning of lichen symbiosis have recently begun to be studied, often by culture-independent techniques due to difficulties in their isolation and culture. However, culturing as yet unculturable lichenic bacteria is critical to unravel their potential functional roles in lichen symbiogenesis, to explore and exploit their biotechnological potential and for the description of new taxa...
2016: PloS One
https://www.readbyqxmd.com/read/27206701/evaluation-of-antioxidant-and-cytotoxic-effects-of-olivetoric-and-physodic-acid-in-cultured-human-amnion-fibroblasts
#10
B Emsen, H Turkez, B Togar, A Aslan
It is known that lichens are utilized for the treatment of many diseases including ulcer, diabetes, and cancer for many years. Secondary metabolites in the structure of the lichens provide various activity properties for them. In the present study, cytotoxic and oxidative effects of main constituents of Pseudevernia furfuracea (L.) Zopf (Parmeliaceae), olivetoric acid (OA), and physodic acid (PA) were investigated on cultured human amnion fibroblasts (HAFs). OA and PA were isolated from P. furfuracea using column chromatography and their structures were determined by proton nuclear magnetic resonance and carbon-13 nuclear magnetic resonance...
April 2017: Human & Experimental Toxicology
https://www.readbyqxmd.com/read/26838439/sample-preparation-for-an-optimized-extraction-of-localized-metabolites-in-lichens-application-to-pseudevernia-furfuracea
#11
Sarah Komaty, Marine Letertre, Huyen Duong Dang, Harald Jungnickel, Peter Laux, Andreas Luch, Daniel Carrié, Odile Merdrignac-Conanec, Jean-Pierre Bazureau, Fabienne Gauffre, Sophie Tomasi, Ludovic Paquin
Lichens are symbiotic organisms known for producing unique secondary metabolites with attractive cosmetic and pharmacological properties. In this paper, we investigated three standard methods of preparation of Pseudevernia furfuracea (blender grinding, ball milling, pestle and mortar). The materials obtained were characterized by electronic microscopy, nitrogen adsorption and compared from the point of view of extraction. Their microscopic structure is related to extraction efficiency. In addition, it is shown using thalline reactions and mass spectrometry mapping (TOF-SIMS) that these metabolites are not evenly distributed throughout the organism...
April 1, 2016: Talanta
https://www.readbyqxmd.com/read/26704132/in-vitro-antitumor-activities-of-the-lichen-compounds-olivetoric-physodic-and-psoromic-acid-in-rat-neuron-and-glioblastoma-cells
#12
Bugrahan Emsen, Ali Aslan, Basak Togar, Hasan Turkez
Context Since methods utilised in the treatment of glioblastoma multiforme (GBM) are inadequate and have too many side effects, usage of herbal products in the treatment process comes into prominence. Lichens are symbiotic organisms used for medicinal purposes for many years. There are various anticancer treatments about components of two lichen species used in the present study. Objective Antitumor potential of three lichen secondary metabolites including olivetoric acid (OLA) and physodic acid (PHA) isolated from Pseudevernia furfuracea (L...
September 2016: Pharmaceutical Biology
https://www.readbyqxmd.com/read/26546421/spatial-variation-in-the-accumulation-of-elements-in-thalli-of-the-lichen-pseudevernia-furfuracea-l-zopf-transplanted-around-a-biomass-power-plant-in-italy
#13
Lucio Lucadamo, Anna Corapi, Stefano Loppi, Rosanna De Rosa, Donatella Barca, Giovanni Vespasiano, Luana Gallo
Thalli of the lichen Pseudevernia furfuracea were transplanted for 3 months at 32 sites located in and around an industrial area of S Italy whose main anthropogenic sources of atmospheric trace elements are a biomass power plant and vehicular emissions. Meteorological stations were deployed at four sites for finer detection of local wind patterns. The station near the biomass power plant showed a significant S-SE wind component not detectable by measurements made at the regional scale or by the other local meteorological stations...
April 2016: Archives of Environmental Contamination and Toxicology
https://www.readbyqxmd.com/read/26417313/platismatia-glaucia-and-pseudevernia-furfuracea-lichens-as-sources-of-antioxidant-antimicrobial-and-antibiofilm-agents
#14
Tatjana Mitrovic, Slaviša Stamenkovic, Vladimir Cvetkovic, Niko Radulovic, Marko Mladenovic, Milan Stankovic, Marina Topuzovic, Ivana Radojevic, Olgica Stefanovic, Sava Vasic, Ljiljana Comic
The antioxidative, antimicrobial and antibiofilm potentials of acetone, ethyl acetate and methanol extracts of lichen species Platismatia glauca and Pseudevernia furfuracea were evaluated. The phytochemical analysis by GC, GC/MS and NMR revealed caperatic acid, atraric acid, atranorin and chloroatranorin as the predominant compounds in Platismatia glauca. Atraric acid, olivetoric acid, atranorin and chloroatranorin were the major constituents in Pseudevernia furfuracea. The strong antioxidant capacities of the Platismatia glauca and Pseudevernia furfuracea extracts were assessed by their total phenolic and flavonoid contents and DPPH scavenging activities...
2014: EXCLI Journal
https://www.readbyqxmd.com/read/26160739/spatial-variation-of-eco-physiological-parameters-in-the-lichen-pseudevernia-furfuracea-transplanted-in-an-area-surrounding-a-cement-plant-s-italy
#15
Lucio Lucadamo, Anna Corapi, Stefano Loppi, Luca Paoli, Luana Gallo
Thalli of the lichen Pseudevernia furfuracea were transplanted for 3 months (November 2010-January 2011) at 61 monitoring sites around a cement plant near Castrovillari (Calabria, southern Italy). NH3, NO x and SO2 concentrations were monitored monthly in a subarea of 10 sites (SA10) where the cement plant was located. At the end of the exposure period, the integrity of cell membranes; membrane lipid peroxidation (thiobarbituric acid reactive substances, TBARS level); vitality (cell respiration); chlorophyll a; chlorophyll b; carotenoids; phaeophytization quotient; photosynthetic efficiency and thalli concentrations of Al, Ca, Mg, V and Fe were measured...
August 2015: Environmental Monitoring and Assessment
https://www.readbyqxmd.com/read/25973860/nir-spectroscopy-as-a-tool-for-discriminating-between-lichens-exposed-to-air-pollution
#16
Monica Casale, Lucia Bagnasco, Paolo Giordani, Mauro Giorgio Mariotti, Paola Malaspina
Lichens are used as biomonitors of air pollution because they are extremely sensitive to the presence of substances that alter atmospheric composition. Fifty-one thalli of two different varieties of Pseudevernia furfuracea (var. furfuracea and var. ceratea) were collected far from local sources of air pollution. Twenty-six of these thalli were then exposed to the air for one month in the industrial port of Genoa, which has high levels of environmental pollution. The possibility of using Near-infrared spectroscopy (NIRS) for generating a 'fingerprint' of lichens was investigated...
September 2015: Chemosphere
https://www.readbyqxmd.com/read/25966238/application-of-molecular-markers-to-detect-dna-damage-caused-by-environmental-pollutants-in-lichen-species
#17
D Cansaran-Duman, E Altunkaynak, A Aslan, I Büyük, S Aras
Pseudevernia furfuracea L. (Zopf), Peltigera praetextata (Flörke ex Sommerf.) Zopf, Lobaria pulmonaria (L.) Hoffm., and Usnea longissima Ach. lichen species were used as bioindicators to assess the genotoxicity of air pollutants. In the present study, we examined significant environmetal pollutants and investigate how changes may lead to damage in DNA structure using RAPD markers. In the study area (Erzurum, Turkey), poor-quality lignite, which generates a large amount of sulfur dioxide, nitrogen oxides, and particle matter, is used for domestic heating, and vehicles also contribute to air pollution...
2015: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/25220142/dereplication-of-depsides-from-the-lichen-pseudevernia-furfuracea-by-centrifugal-partition-chromatography-combined-to-13c-nuclear-magnetic-resonance-pattern-recognition
#18
Sarah K Oettl, Jane Hubert, Jean-Marc Nuzillard, Hermann Stuppner, Jean-Hugues Renault, Judith M Rollinger
Lichens produce a diversity of secondary metabolites, among them depsides comprised of two or more hydroxybenzoic acid units linked by ester, ether, or CC-bonds. During classic solid support-based purification processes, depsides are often hydrolyzed and in many cases time, consuming procedures result only in the isolation of decomposition products. In an attempt to avoid extensive purification steps while maintaining metabolite structure integrity, we propose an alternative method to identify the major depsides of a lichen crude extract (Pseudevernia furfuracea var...
October 10, 2014: Analytica Chimica Acta
https://www.readbyqxmd.com/read/24971640/estimating-atmospheric-mercury-concentrations-with-lichens
#19
COMPARATIVE STUDY
Andrea Vannini, Valentina Nicolardi, Roberto Bargagli, Stefano Loppi
The uptake kinetics of elemental gaseous Hg (Hg(0)) in three species of epiphytic lichens (Pseudevernia furfuracea, Evernia prunastri, and Xanthoria parietina) were investigated under four different Hg concentrations (10, 15, 30, and 45 μg/m(3)) and three different temperatures (10, 20, and 30 °C) with the aim of evaluating the lichen efficiency for Hg(0) accumulation and their potential use in the estimate of atmospheric concentrations of this metal in the field. The results showed that under our experimental conditions the lichens accumulated Hg according to exposure time and that the metal is not released back to the atmosphere after Hg(0) was removed from the air (clearance)...
2014: Environmental Science & Technology
https://www.readbyqxmd.com/read/24442534/temporal-trends-of-element-concentrations-and-ecophysiological-parameters-in-the-lichen-pseudevernia-furfuracea-transplanted-in-and-around-an-industrial-area-of-s-italy
#20
A Corapi, L Gallo, V Nicolardi, L Lucadamo, S Loppi
This study investigated the temporal trends of element concentrations and ecophysiological parameters (integrity of cell membranes, membrane lipid peroxidation, and sample vitality) in thalli of the lichen Pseudevernia furfuracea transplanted in and around an industrial area of S Italy from May 2010 to May 2011, once every 3 months. Element accumulation at levels indicating that the atmosphere of the study area was polluted by most elements occurred only during the third trimester of exposure, and the ecophysiological parameter best reflecting this trend was sample vitality expressed in terms of activity of the enzyme dehydrogenase...
May 2014: Environmental Monitoring and Assessment
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