Stefano Nebbia, Cristina Lamberti, Simona Cirrincione, Alberto Acquadro, Simona Abbà, Marina Ciuffo, Daniela Torello Marinoni, Marcello Manfredi, Emilio Marengo, Roberta Calzedda, Giovanna Monti, Laura Cavallarin, Maria Gabriella Giuffrida
BACKGROUND: Hazelnut allergy, which is characterized by symptoms that range from mild to severe, is one of the most common allergies in children throughout Europe, and an accurate diagnosis of this allergy is therefore essential. However, lipophilic allergens, such as oleosins, are generally underrepresented in diagnostic tests. We therefore sought to characterize the IgE reactivity of raw and roasted hazelnut oleosins, using the sera of hazelnut-allergic pediatric patients. METHODS: Raw and roasted hazelnut oil body-associated proteins were analyzed by means of 1D and 2D electrophoresis and MS...
November 2021: Pediatric Allergy and Immunology