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https://www.readbyqxmd.com/read/28849911/dna-encoded-raman-active-anisotropic-nanoparticles-for-microrna-detection
#1
Lin Qi, Mingshu Xiao, Xiwei Wang, Cheng Wang, Lihua Wang, Shiping Song, Xiangmeng Qu, Li Li, Jiye Shi, Hao Pei
The development of highly sensitive and selective methods for the detection of microRNA (miRNA) has attracted tremendous attention because of its importance in fundamental biological studies and diagnostic applications. In this work, we develop DNA-encoded Raman-active anisotropic nanoparticles modified origami paper analytical devices (oPADs) for rapid, highly sensitive, and specific miRNA detection. The Raman-active anisotropic nanoparticles were prepared using 10-mer oligo-A, -T, -C, and -G to mediate the growth of Ag cubic seeds into Ag nanoparticles (AgNPs) with different morphologies...
August 29, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28816436/integrated-distance-based-origami-paper-analytical-device-for-one-step-visualized-analysis
#2
Tian Tian, Yuan An, Yiping Wu, Yanling Song, Zhi Zhu, Chaoyong Yang
An integrated distance-based origami paper analytical device (ID-oPAD) is developed for simple, user friendly and visual detection of targets of interest. The platform enables complete integration of target recognition, signal amplification, and visual signal output based on aptamer/invertase-functionalized sepharose beads, cascaded enzymatic reactions, and a 3D microfluidic paper-based analytical device with distance-based readout, respectively. The invertase-DNA conjugate is released upon target addition, after which it permeates through the cellulose and flows down into the bottom detection zone, whereas sepharose beads with larger size are excluded and stay in the upper zone...
September 13, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28643911/effects-of-cocoa-enriched-diet-on-orofacial-pain-in-a-murine-model
#3
L N Bowden, E L Rohrs, K Omoto, P L Durham, L S Holliday, A D Morris, K D Allen, R M Caudle, J K Neubert
OBJECTIVES: To investigate and discuss the effects of cocoa on orofacial pain. SETTING AND SAMPLE POPULATION: The Department of Orthodontics at the University of Florida (UF). Male and female hairless rats (N=20/group) were tested. MATERIALS AND METHODS: Rats were tested using the Orofacial Pain Assessment Device (OPAD) before and after changing their food from the standard chow to a cocoa-enriched or control-equivalent diet. RESULTS: Male rats fed the cocoa diet had a significantly higher operant pain index when tested at 37°C as compared to control diet-fed animals...
June 2017: Orthodontics & Craniofacial Research
https://www.readbyqxmd.com/read/27754087/os-20-01-a-high-normal-ankle-brachial-index-is-associated-with-arterial-stiffness-and-target-organ-damage-the-okinawa-peripheral-arterial-disease-study-opads
#4
Akio Ishida, Yoshino Kinjo, Yuichiro Toma, Kozen Kinjo, Kunitoshi Iseki, Yusuke Ohya
OBJECTIVE: We reported that ankle-brachial index (ABI) was lowest at <40 years, and increased with age until 60 years and decreased thereafter in a screened cohort of Japanese general population. We hypothesized that this increase in ABI with age occurs owing to increasing arterial stiffness and wave reflection, and therefore, a high normal ABI is associated with target organ damage. DESIGN AND METHOD: We measured ABI and brachial-ankle pulse wave velocity (baPWV) by an automatic oscillometric method...
September 2016: Journal of Hypertension
https://www.readbyqxmd.com/read/27643012/os-20-01-a-high-normal-ankle-brachial-index-is-associated-with-arterial-stiffness-and-target-organ-damage-the-okinawa-peripheral-arterial-disease-study-opads
#5
Akio Ishida, Yoshino Kinjo, Yuichiro Toma, Kozen Kinjo, Kunitoshi Iseki, Yusuke Ohya
OBJECTIVE: We reported that ankle-brachial index (ABI) was lowest at <40 years, and increased with age until 60 years and decreased thereafter in a screened cohort of Japanese general population. We hypothesized that this increase in ABI with age occurs owing to increasing arterial stiffness and wave reflection, and therefore, a high normal ABI is associated with target organ damage. DESIGN AND METHOD: We measured ABI and brachial-ankle pulse wave velocity (baPWV) by an automatic oscillometric method...
September 2016: Journal of Hypertension
https://www.readbyqxmd.com/read/25815042/a-clinical-decision-support-system-for-the-diagnosis-fracture-risks-and-treatment-of-osteoporosis
#6
Bjarni V Halldorsson, Aron Hjalti Bjornsson, Haukur Tyr Gudmundsson, Elvar Orn Birgisson, Bjorn Runar Ludviksson, Bjorn Gudbjornsson
Expanding medical knowledge increases the potential risk of medical errors in clinical practice. We present, OPAD, a clinical decision support system in the field of the medical care of osteoporosis. We utilize clinical information from international guidelines and experts in the field of osteoporosis. Physicians are provided with user interface to insert standard patient data, from which OPAD provides instant diagnostic comments, 10-year risk of fragility fracture, treatment options for the given case, and when to offer a follow-up DXA-evaluation...
2015: Computational and Mathematical Methods in Medicine
https://www.readbyqxmd.com/read/25456275/low-voltage-origami-paper-based-electrophoretic-device-for-rapid-protein-separation
#7
Long Luo, Xiang Li, Richard M Crooks
We present an origami paper-based electrophoretic device (oPAD-Ep) that achieves rapid (∼5 min) separation of fluorescent molecules and proteins. Due to the innovative design, the required driving voltage is just ∼10 V, which is more than 10 times lower than that used for conventional electrophoresis. The oPAD-Ep uses multiple, thin (180 μm/layer) folded paper layers as the supporting medium for electrophoresis. This approach significantly shortens the distance between the anode and cathode, and this, in turn, accounts for the high electric field (>1 kV/m) that can be achieved even with a low applied voltage...
December 16, 2014: Analytical Chemistry
https://www.readbyqxmd.com/read/25346239/opa-neisseria-gonorrhoeae-exhibits-reduced-survival-in-human-neutrophils-via-src-family-kinase-mediated-bacterial-trafficking-into-mature-phagolysosomes
#8
M Brittany Johnson, Louise M Ball, Kylene P Daily, Jennifer N Martin, Linda Columbus, Alison K Criss
During gonorrhoeal infection, there is a heterogeneous population of Neisseria gonorrhoeae (Gc) varied in their expression of opacity-associated (Opa) proteins. While Opa proteins are important for bacterial attachment and invasion of epithelial cells, Opa+ Gc has a survival defect after exposure to neutrophils. Here, we use constitutively Opa- and OpaD+ Gc in strain background FA1090 to show that Opa+ Gc is more sensitive to killing inside adherent, chemokine-treated primary human neutrophils due to increased bacterial residence in mature, degradative phagolysosomes that contain primary and secondary granule antimicrobial contents...
May 2015: Cellular Microbiology
https://www.readbyqxmd.com/read/24963078/feeding-tubes-for-older-people-with-advanced-dementia-living-in-the-community-in-israel
#9
Netta Bentur, Shelley Sternberg, Jennifer Shuldiner, Tzvi Dwolatzky
BACKGROUND: Feeding tubes to address eating problems of older people with advanced dementia (OPAD) has been studied primarily in nursing homes. OBJECTIVES: To examine the prevalence of feeding tube use among OPAD living in the community; to evaluate the characteristics, quality of care, and the burden on caregivers. METHODS: A cross-sectional survey of 117 caregivers of OPAD living in the community. RESULTS: Of 117 patients, 26% had feeding tubes...
March 2015: American Journal of Alzheimer's Disease and Other Dementias
https://www.readbyqxmd.com/read/24625315/wire-mesh-and-fiber-electrodes-for-paper-based-electroanalytical-devices
#10
Stephen E Fosdick, Morgan J Anderson, Christophe Renault, Paul R DeGregory, James A Loussaert, Richard M Crooks
Here, we report the use of microwire and mesh working electrodes in paper analytical devices fabricated by origami paper folding (oPADs). The important new result is that Au wires and carbon fibers having diameters ranging from micrometers to tens of micrometers can be incorporated into oPADs and that their electrochemical characteristics are consistent with the results of finite element simulations. These electrodes are fully compatible with both hollow channels and paper channels filled with cellulose fibers, and they are easier to incorporate than typical screen-printed carbon electrodes...
April 1, 2014: Analytical Chemistry
https://www.readbyqxmd.com/read/24558480/the-effects-of-a-co-application-of-menthol-and-capsaicin-on-nociceptive-behaviors-of-the-rat-on-the-operant-orofacial-pain-assessment-device
#11
Ethan M Anderson, Alan C Jenkins, Robert M Caudle, John K Neubert
BACKGROUND: Transient receptor potential (TRP) cation channels are involved in the perception of hot and cold pain and are targets for pain relief in humans. We hypothesized that agonists of TRPV1 and TRPM8/TRPA1, capsaicin and menthol, would alter nociceptive behaviors in the rat, but their opposite effects on temperature detection would attenuate one another if combined. METHODS: Rats were tested on the Orofacial Pain Assessment Device (OPAD, Stoelting Co.) at three temperatures within a 17 min behavioral session (33°C, 21°C, 45°C)...
2014: PloS One
https://www.readbyqxmd.com/read/24438033/quality-of-care-of-older-people-living-with-advanced-dementia-in-the-community-in-israel
#12
MULTICENTER STUDY
Shelley Sternberg, Netta Bentur, Jennifer Shuldiner
OBJECTIVES: To examine the quality of end-of-life (EOL) care of older people with advanced dementia (OPAD) living in the community. DESIGN: Retrospective cohort study. SETTING: The second largest preferred provider organization in Israel. PARTICIPANTS: Primary family caregivers of OPAD (N = 117; 52 bereaved caregivers, 65 still caring for OPAD). MEASUREMENTS: Primary outcome measures were quality-of-EOL-care scales regarding symptom management at the EOL with dementia (SM-EOLD) and comfort assessment in dying at the EOL with dementia (CAD-EOLD)...
February 2014: Journal of the American Geriatrics Society
https://www.readbyqxmd.com/read/24343654/assembly-of-nadph-oxidase-in-human-neutrophils-is-modulated-by-the-opacity-associated-protein-expression-state-of-neisseria-gonorrhoeae
#13
Asya Smirnov, Kylene P Daily, Alison K Criss
Neisseria gonorrhoeae (the gonococcus, Gc) triggers a potent inflammatory response and recruitment of neutrophils to the site of infection. Gc survives exposure to neutrophils despite these cells' antimicrobial products, such as reactive oxygen species (ROS). ROS production in neutrophils is initiated by NADPH oxidase, which converts oxygen into superoxide. The subunits of NADPH oxidase are spatially separated between granules (gp91(phox)/p22(phox)) and the cytoplasm (p47(phox), p67(phox), and p40(phox)). Activation of neutrophils promotes the coassembly of NADPH oxidase subunits at phagosome and/or plasma membranes...
March 2014: Infection and Immunity
https://www.readbyqxmd.com/read/24070108/dna-detection-using-origami-paper-analytical-devices
#14
Karen Scida, Bingling Li, Andrew D Ellington, Richard M Crooks
We demonstrate the hybridization-induced fluorescence detection of DNA on an origami-based paper analytical device (oPAD). The paper substrate was patterned by wax printing and controlled heating to construct hydrophilic channels and hydrophobic barriers in a three-dimensional fashion. A competitive assay was developed where the analyte, a single-stranded DNA (ssDNA), and a quencher-labeled ssDNA competed for hybridization with a fluorophore-labeled ssDNA probe. Upon hybridization of the analyte with the fluorophore-labeled ssDNA, a linear response of fluorescence vs analyte concentration was observed with an extrapolated limit of detection <5 nM and a sensitivity relative standard deviation as low as 3%...
October 15, 2013: Analytical Chemistry
https://www.readbyqxmd.com/read/23977246/phase-variation-leads-to-the-misidentification-of-a-neisseria-gonorrhoeae-virulence-gene
#15
Mark T Anderson, H Steven Seifert
Neisseria gonorrhoeae is the causative agent of gonorrhea and an obligate pathogen of humans. The Opa proteins of these bacteria are known to mediate attachment and internalization by host cells, including neutrophils. The Opa protein repertoire of a typical N. gonorrhoeae isolate is encoded on ~11 genes distributed throughout the chromosome and is subject to stochastic changes in expression through phase variation. Together, these characteristics make Opa proteins a critical yet unpredictable aspect of any experimental investigation into the interaction of N...
2013: PloS One
https://www.readbyqxmd.com/read/23792907/use-of-the-operant-orofacial-pain-assessment-device-opad-to-measure-changes-in-nociceptive-behavior
#16
Ethan M Anderson, Richard Mills, Todd A Nolan, Alan C Jenkins, Golam Mustafa, Chris Lloyd, Robert M Caudle, John K Neubert
We present an operant system for the detection of pain in awake, conscious rodents. The Orofacial Pain Assessment Device (OPAD) assesses pain behaviors in a more clinically relevant way by not relying on reflex-based measures of nociception. Food fasted, hairless (or shaved) rodents are placed into a Plexiglas chamber which has two Peltier-based thermodes that can be programmed to any temperature between 7 °C and 60 °C. The rodent is trained to make contact with these in order to access a reward bottle. During a session, a number of behavioral pain outcomes are automatically recorded and saved...
2013: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/23625842/constitutively-opa-expressing-and-opa-deficient-neisseria-gonorrhoeae-strains-differentially-stimulate-and-survive-exposure-to-human-neutrophils
#17
Louise M Ball, Alison K Criss
The Neisseria gonorrhoeae (the gonococcus [Gc]) opacity-associated (Opa) proteins mediate bacterial binding and internalization by human epithelial cells and neutrophils (polymorphonuclear leukocytes [PMNs]). Investigating the contribution of Opa proteins to gonococcal pathogenesis is complicated by high-frequency phase variation of the opa genes. We therefore engineered a derivative of Gc strain FA1090 in which all opa genes were deleted in frame, termed Opaless. Opaless Gc remained uniformly Opa negative (Opa(-)), whereas cultures of predominantly Opa(-) parental Gc and an intermediate lacking the "translucent" subset of opa genes (ΔopaBEGK) stochastically gave rise to Opa-positive (Opa(+)) bacterial colonies...
July 2013: Journal of Bacteriology
https://www.readbyqxmd.com/read/23033545/age-and-sex-related-effects-on-ankle-brachial-index-in-a-screened-cohort-of-japanese-the-okinawa-peripheral-arterial-disease-study-opads
#18
Akio Ishida, Megumi Miyagi, Kozen Kinjo, Yusuke Ohya
AIMS: Age-related change of ankle-brachial index (ABI) within the general population, especially <40 years, has not been determined in large population studies. We evaluated the value of the ABI by age- and sex-related differences in a screened cohort. METHODS AND RESULTS: The ABI was examined in 13,211 participants (aged 21-89 years) in a health evaluation programme. The mean ABI was lower in women than in men at all ages. The ABI was lowest at <40 years, and increased with age; the maximum was at 60-69 years in both sexes...
June 2014: European Journal of Preventive Cardiology
https://www.readbyqxmd.com/read/21234232/molecular-markers-for-identification-of-stellantchasmus-falcatus-and-a-phylogenic-study-using-the-hat-rapd-method
#19
Chalobol Wongsawad, Pheravut Wongsawad
Stellantchasmus falcatus is a minute intestinal fluke in the family Heterophyidae. Metacercariae, the infective stage, were reported in a marine fish, mullet Liza subviridis, and a fresh water fish, Dermogenus pusillus, in Thailand. Adults were found in chicks, rats, cats, and humans. Morphological studies were done for comparing Stellantchasmus sp. worms found in 2 different fish hosts; their shapes and organ arrangements were very similar except for the prepharynx length. Therefore, the present study aimed to compare their DNA fingerprints using the HAT-RAPD method for both types of Stellantchasmus and several other related species...
December 2010: Korean Journal of Parasitology
https://www.readbyqxmd.com/read/19413110/genetic-and-withaferin-a-analysis-of-iranian-natural-populations-of-withania-somnifera-and-w-coagulans-by-rapd-and-hptlc
#20
Mohammad Hossein Mirjalili, Seyyed Mohammad Fakhr-Tabatabaei, Houshang Alizadeh, Alireza Ghassempour, Fateme Mirzajani
For successful conservation and breeding of a medicinal species, it is important to evaluate its genetic diversity as well as its content of phytochemical compounds. The aim of the present study was to investigate the genetic variation of Iranian natural populations of W. somnifera and W. coagulans, using the RAPD (random amplified polymorphic DNA) markers, and their withaferin A content. Using 16 RAPD primers, a total of 282 RAPD bands were achieved. The highest and lowest percentages of polymorphism were observed with primers OPAD-15 (100...
March 2009: Natural Product Communications
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