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Retina And Quantitative

William Robert Kwapong, Hua Ye, Chenlei Peng, Xiran Zhuang, Jianhua Wang, Meixiao Shen, Fan Lu
Purpose: To detect the retinal microvascular impairment using optical coherence tomography angiography (OCT-A) in patients with Parkinson's disease (PD) and find a correlation between the microvascular impairment and the neuronal damage. Methods: This is a prospective, observational study including 49 eyes from 38 PD patients in their early stages and 34 eyes from 28 healthy controls with comparable age range. Macula microvasculature was evaluated with the spectral-domain optical coherence tomography (SD-OCT) angiography and intraretinal layer thickness evaluated with the SD-OCT...
August 1, 2018: Investigative Ophthalmology & Visual Science
Karina Kaczmarek-Hajek, Jiong Zhang, Robin Kopp, Antje Grosche, Björn Rissiek, Anika Saul, Santina Bruzzone, Tobias Engel, Tina Jooss, Anna Krautloher, Stefanie Schuster, Tim Magnus, Christine Stadelmann, Swetlana Sirko, Friedrich Koch-Nolte, Volker Eulenburg, Annette Nicke
The P2X7 channel is involved in the pathogenesis of various CNS diseases. An increasing number of studies suggest its presence in neurons where its putative functions remain controversial for more than a decade. To resolve this issue and to provide a model for analysis of P2X7 functions, we generated P2X7-BAC transgenic mice that allow visualization of functional EGFP-tagged P2X7 receptors in vivo . Extensive characterization of these mice revealed dominant P2X7-EGFP protein expression in microglia, Bergmann glia, and oligodendrocytes, but not in neurons...
August 3, 2018: ELife
Kota Sato, Yukihiro Shiga, Yurika Nakagawa, Kosuke Fujita, Koji M Nishiguchi, Hiroshi Tawarayama, Namie Murayama, Shigeto Maekawa, Takeshi Yabana, Kazuko Omodaka, Shota Katayama, Qiwei Feng, Satoru Tsuda, Toru Nakazawa
Purpose: To assess the therapeutic potential of endothelin-converting enzyme-like 1 (Ecel1) in a mouse model of optic nerve crush. Methods: Ecel1 expression was evaluated with real time quantitative (qRT)-PCR, Western blotting, and immunohistochemistry in mouse retinas after optic nerve crush. Vinblastine administration to the optic nerve and the intravitreal injection of N-methyl-d-aspartate (NMDA) were used to assess Ecel1 gene expression. Ecel1 was deleted with an adeno-associated viral (AAV) clustered regulatory interspaced short palindromic repeat (CRISPR)/Cas9 system, and retinal ganglion cell (RGC) survival was investigated with retrograde labeling, qRT-PCR, and visual evoked potential...
August 1, 2018: Investigative Ophthalmology & Visual Science
Patrick Vancura, Erika Csicsely, Annalisa Leiser, P Michael Iuvone, Rainer Spessert
Purpose: The aim of the present study was to identify candidate genes for mediating daily adjustment of vision. Methods: Genes important for vision and genetically associated with severe retinal diseases were tested for 24-hour rhythms in transcript levels in neuronal retina, microdissected photoreceptors, photoreceptor-related pinealocytes, and retinal pigment epithelium-choroid (RPE-choroid) complex by using quantitative PCR. Results: Photoreceptors of wildtype mice display circadian clock-dependent regulation of visual arrestins (Arr1, Arr4) and the visual cycle gene Rdh12, whereas cells of the RPE-choroid exhibit light-dependent regulation of the visual cycle key genes Lrat, Rpe65, and Rdh5...
August 1, 2018: Investigative Ophthalmology & Visual Science
Qing Li Zhang, Shuang Liu, Jun Li, Ting Ting Xu, Xiu Hua Wang, Guang Ming Fu, Xiao Ping Li, Song Wen Sang, Xiao Dong Bian, Jing Wei Hao
Viral covert mortality disease (VCMD), caused by covert mortality nodavirus (CMNV), is a newly emerging disease affecting most cultured shrimp and other crustaceans, but not fish. However, we discovered for the first time that Mugilogobius abei , a common marine fish collecting from shrimp farming ponds and surrounding coastal waters in China, was tested to be CMNV positive based on reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay. Further investigation based on the quantitative RT-LAMP assay indicated that 39% individuals of sampled M...
2018: Frontiers in Microbiology
Brian C Tse, Galina Dvoriantchikova, Wensi Tao, Ryan A Gallo, John Y Lee, Steven Pappas, Roberta Brambilla, Dmitry Ivanov, David T Tse, Daniel Pelaez
Purpose: To determine the effectiveness of etanercept, a tumor necrosis factor (TNF) inhibitor, in conferring neuroprotection to retinal ganglion cells (RGCs) and improving visual outcomes after optic nerve trauma with either optic nerve crush (ONC) or sonication-induced traumatic optic neuropathy (SI-TON) in mice. Methods: Mouse optic nerves were unilaterally subjected to ONC (n = 20) or SI-TON (n = 20). TNF expression was evaluated by using immunohistochemistry and quantitative RT-PCR (qRT-PCR) in optic nerves harvested 6 and 24 hours post ONC (n = 10) and SI-TON (n = 10)...
June 1, 2018: Investigative Ophthalmology & Visual Science
Neha Anegondi, Arpita Kshirsagar, Thirumalesh B Mochi, Abhijit Sinha Roy
BACKGROUND AND OBJECTIVE: To compare optical coherence tomography angiography (OCTA) images from three different devices. PATIENTS AND METHODS: This was a prospective, observational, cross-sectional study. All eyes (n = 24) were imaged thrice each time with swept-source OCT (DRI OCT Triton Plus; Topcon, Tokyo, Japan), spectral-domain OCTA (AngioVue; Optovue, Fremont, CA), and SD-OCT Angioplex (Cirrus HD-OCT 5000; Carl Zeiss Meditec, Jena, Germany). Outcome measures were foveal avascular zone (FAZ) area, vessel density, spacing between large vessels (SLV), and spacing between small vessels (SSV)...
July 1, 2018: Ophthalmic Surgery, Lasers & Imaging Retina
Jose R Hombrebueno, Imran H A Ali, Jian-Xing Ma, Mei Chen, Heping Xu
AIMS/HYPOTHESIS: Cataract surgery in diabetic individuals worsens pre-existing retinopathy and triggers the development of diabetic ocular complications, although the underlying cellular and molecular pathophysiology remains elusive. We hypothesise that lens surgery may exaggerate pre-existing retinal inflammation in diabetes, which may accelerate neurovascular degeneration in diabetic eyes. METHODS: Male heterozygous Ins2Akita mice (3 months of age) and C57BL/6 J age-matched siblings received either lens capsulotomy (to mimic human cataract surgery) or corneal incision (sham surgery) in the right eye...
July 17, 2018: Diabetologia
Theodore B DuBose, David Cunefare, Elijah Cole, Peyman Milanfar, Joseph A Izatt, Sina Farsiu
Optical coherence tomography (OCT) has revolutionized diagnosis and prognosis of ophthalmic diseases by visualization and measurement of retinal layers. To speed up quantitative analysis of disease biomarkers, an increasing number of automatic segmentation algorithms have been proposed to estimate the boundary locations of retinal layers. While the performance of these algorithms has significantly improved in recent years, a critical question to ask is how far we are from a theoretical limit to OCT segmentation performance...
November 13, 2017: IEEE Transactions on Medical Imaging
Mana Fukuda, Kazuko Omodaka, Yasuko Tatewaki, Noriko Himori, Izumi Matsudaira, Koji M Nishiguchi, Takaki Murata, Yasuyuki Taki, Toru Nakazawa
BACKGROUND: The aims of this study were to investigate glaucomatous morphological changes quantitatively in the visual cortex of the brain with voxel-based morphometry (VBM), a normalizing MRI technique, and to clarify the relationship between glaucomatous damage and regional changes in the visual cortex of patients with open-angle glaucoma (OAG). METHODS: Thirty-one patients with OAG (age: 55.9 ± 10.7, male: female = 9: 22) and 20 age-matched controls (age: 54...
2018: PloS One
Weiye Song, Libo Zhou, Sui Zhang, Steven Ness, Manishi Desai, Ji Yi
Elastic light scattering spectroscopy (ELSS) has been proven a powerful method in measuring tissue structures with exquisite nanoscale sensitivity. However, ELSS contrast in the living human retina has been relatively underexplored, primarily due to the lack of imaging tools with a large spectral bandwidth. Here, we report a simple all fiber-based setup to implement dual-channel visible and near infrared (NIR) optical coherence tomography (vnOCT) for human retinal imaging, bridging over a 300nm spectral gap...
July 1, 2018: Biomedical Optics Express
Qian-Hui Yang, Yan Zhang, Jing Jiang, Mian-Mian Wu, Qian Han, Qi-Yu Bo, Guang-Wei Yu, Yu-Sha Ru, Xun Liu, Min Huang, Ling Wang, Xiao-Min Zhang, Jian-Min Fang, Xiao-Rong Li
AIM: To investigate protective effects of a novel recombinant decoy receptor drug RC28-E on retinal damage in early diabetic rats. METHODS: The streptozotocin (STZ)-induced diabetic rats were randomly divided into 6 groups: diabetes mellitus (DM) group (saline, 3 µL/eye); RC28-E at low (0.33 µg/µL, 3 µL), medium (1 µg/µL, 3 µL), and high (3 µg/µL, 3 µL) dose groups; vascular endothelial growth factor (VEGF) Trap group (1 µg/µL, 3 µL); fibroblast growth factor (FGF) Trap group (1 µg/µL, 3 µL)...
2018: International Journal of Ophthalmology
Kang Wang, Khalil Ghasemi Falavarjani, Muneeswar G Nittala, Min Sagong, Charles C Wykoff, Jano van Hemert, Michael Ip, SriniVas R Sadda
Purpose: The purpose of this study was to compare the use of central and montaged ultra-wide-field fluorescein angiography (UWFFA) images for calculating the area of nonperfusion (NP) and ischemic index (ISI) in patients with retinal vein occlusion (RVO) and macular edema (ME) and to correlate these measurements with best-corrected visual acuity (BCVA) and central macular thickness (CMT). Methods: Thirty eyes of 30 RVO patients with recurrent ME were enrolled. Baseline UWFA images were sent to the Doheny Image Reading Center for quantitative analysis by certified graders...
July 2, 2018: Investigative Ophthalmology & Visual Science
Ursula Schmidt-Erfurth, Sebastian M Waldstein, Sophie Klimscha, Amir Sadeghipour, Xiaofeng Hu, Bianca S Gerendas, Aaron Osborne, Hrvoje Bogunovic
Purpose: While millions of individuals show early age-related macular degeneration (AMD) signs, yet have excellent vision, the risk of progression to advanced AMD with legal blindness is highly variable. We suggest means of artificial intelligence to individually predict AMD progression. Methods: In eyes with intermediate AMD, progression to the neovascular type with choroidal neovascularization (CNV) or the dry type with geographic atrophy (GA) was diagnosed based on standardized monthly optical coherence tomography (OCT) images by independent graders...
July 2, 2018: Investigative Ophthalmology & Visual Science
Weiming Yan, Pan Long, Tao Chen, Wei Liu, Lu Yao, Ze Ren, Xiangqian Li, Jiancong Wang, Junhui Xue, Ye Tao, Lei Zhang, Zuoming Zhang
BACKGROUND/AIMS: Our laboratory discovered a Kunming mouse with enormous electroretinogram (ERG) defects. Its auditory brainstem response (ABR) threshold was significantly elevated and closely resembled the features of Usher syndrome (USH). This study sought to cross these USH-like mice (named KMush/ush mice) with CBA/CaJ mice to establish recombinant inbred strains and identify their phenotypes and genotypes. METHODS: KMush/ush mice were crossed with CBA/CaJ mice to establish inbred strains by sibling mating...
2018: Cellular Physiology and Biochemistry
Tyler Heisler-Taylor, Bongsu Kim, Alana Y Reese, Sumaya Hamadmad, Rania Kusibati, Andy J Fischer, Colleen M Cebulla
Nuclear co-localization labels are critical to ocular research. Among these, the TUNEL assay has been established as a gold standard of cell death and apoptosis. While several validated computer-based methods exist to quantitate these markers, including ImageJ Retina Analysis (RA) Toolkit and ImagePro, none verify the count with the nuclear counter stain to confirm nuclear co-localization. We established a new ImageJ-based automated multichannel thresholding (MCT) method to quantitate nuclear co-localized labeling...
June 28, 2018: Experimental Eye Research
Monica M Sauter, Aaron W Kolb, Curtis R Brandt
The purpose of this study was to characterize cell-specific expression patterns of Toll-like receptors (TLR) in non-human primate (NHP) neural retina tissue. TLR 4, 5, 6, and 7 proteins were detected by immunblotting of macaque retina tissue lysates and quantitative PCR (qPCR) demonstrated TLRs 4-7 mRNA expression. Immunofluorescence (IF) microscopy detected TLRs 4-7 in multiple cell types in macaque neural retina including Muller, retinal ganglion cells (RGC), amacrine, and bipolar cells. These results demonstrate that TLRs 4-7 are constitutively expressed by neurons in the NHP retina raising the possibility that these cells could be involved in retinal innate inflammatory responses...
September 15, 2018: Journal of Neuroimmunology
Jinglin Cui, Lidi Liu, Hang Lu, Dongmei Wei, Yan Jiao, Monica M Jablonski, Robert W Williams, Weikuan Gu, Hong Chen
This study's goal is to determine similarities and differences in the molecular pathways or potential functions of the various targeted regions or genes of the Vegf family-VegfA, VegfB, VegfC, and Pgf-using the BXD genetic reference panel. Data from whole genome expression profiles of retinas from the well-characterized mouse recombinant inbred (RI) strain population derived from C57BL/6J X DBA/2J (BXD) were analyzed. Multiple analytical tools and statistical strategies were used to investigate the expression level...
June 23, 2018: Experimental Eye Research
Yueqing He, Shaoxue Zeng, Yunni Zhang, Junjun Zhang
BACKGROUND AND OBJECTIVE: This systematic review and meta-analysis was made to measure risk factors for retinal redetachment (re-RD) after silicone oil removal (SOR) in a quantitative method. PATIENTS AND METHODS: A comprehensive literature review relating to risk factors for re-RD after SOR was conducted before March 2017. Odds ratio (OR) with 95% confidence interval (CI) was calculated after data combination. RESULTS: Sixteen studies were included, and risk factors with significant differences found between the re-RD and control groups are as follows: aphakic eye (OR = 1...
June 1, 2018: Ophthalmic Surgery, Lasers & Imaging Retina
Christina L Rettinger, Heuy-Ching Wang
PURPOSE: Given that porcine and human retinas have similar structures and characteristics, ex vivo culture of porcine neuroretina provides an attractive model for studying mechanisms of human retinal injury and degenerative disease. Here, we describe the method that was used to establish and characterize an adult porcine retina culture system as a rapid screening tool for retinal survival in real time. METHODS: Neuroretina explants 8 mm in diameter were harvested from adult swine and cultured on porous cell culture inserts with adjustable heights...
June 20, 2018: Journal of Ocular Pharmacology and Therapeutics
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