Feifei Guan, Xiaoqian Tian, Ruohan Zhang, Yan Zhang, Ningfeng Wu, Jilu Sun, Honglian Zhang, Tao Tu, Huiying Luo, Bin Yao, Jian Tian, Huoqing Huang
Thermophilic endo-chitinases are essential for production of highly polymerized chitooligosaccharides, which are advantageous for plant immunity, animal nutrition and health. However, thermophilic endo-chitinases are scarce and the transformation from exo- to endo-activity of chitinases is still a challenging problem. In this study, to enhance the endo-activity of the thermophilic chitinase Chi304, we proposed two approaches for rational design based on comprehensive structural and evolutionary analyses. Four effective single-point mutants were identified among 28 designed mutations...
March 7, 2024: Bioresources and Bioprocessing