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https://www.readbyqxmd.com/read/28103575/piwil2-transfected-human-fibroblasts-are-cancer-stem-cell-like-and-genetically-unstable
#1
Deying Zhang, Xin Wu, Xing Liu, Chunhong Cai, Guangping Zeng, Jan Rohozinski, Yuanyuan Zhang, Guanghui Wei, Dawei He
Uncontrolled cell proliferation and inhibition of apoptosis are considered to be vital for cancer initiation, maintenance, infiltration, metastasis and recurrence after anti-cancer therapy. Here we report the generation of a novel cell line by reprogramming child foreskin fibroblast with the full length apoptosis inhibitor gene PIWIL2. The fibroblasts transfected with PIWIL2 expressed the stem cell markers OCT-4, NANOG, SOX-2, KLF-4 and C-MYC; endoderm marker AFP and GATA6; mesoderm markers ACTA2 and BRACHYURY; and ectoderm markers NESTIN and TUBB3...
January 17, 2017: Oncotarget
https://www.readbyqxmd.com/read/28095111/increased-numb-protein-expression-predicts-poor-clinical-outcomes-in-esophageal-squamous-cell-carcinoma-patients
#2
Zhenguo Liu, Canqiao Luo, Weixiong Yang, Kefeng Wang, Chuang Hu, Jianyong Zou, Hua Zhu, Liwu Fu, Jing Nie, Chao Cheng
Numb is a protein whose asymmetric segregation during cell division determines cell fate and has numerous functions relevant to multiple fields of study, including developmental neurobiology and cancer biology. Little is known about the role of Numb in esophageal squamous cell carcinoma (ESCC), the predominant histological esophageal carcinoma in Asian populations. In this study, we focused on the expression and biological functions of Numb in the context of ESCC. From analysis of tissue microarrays with 212 patients, it was found that Numb was significantly upregulated in ESCC tissues compared to corresponding non-cancerous tissues...
January 17, 2017: Cancer Biology & Therapy
https://www.readbyqxmd.com/read/28078252/the-evaluation-of-the-effect-of-herbal-extract-on-osteoarthritis-in-vitro-and-in-vivo-study
#3
Jaeyong Kim, Siyoung Yang, Chul-Yung Choi
In this study, the anti-osteoarthritis effects of Cynanchum wilfordii, Phlomis umbrosa, and Angelica gigas extract (CPAE), observed and confirmed in previously clinical studies were further investigated by in vitro and in vivo studies. Anabolic biomarkers related to healthy cartilage maintenance, such as aggrecan, type II collagen α-1 (Col2a1), sex determining region Y-box-9 (Sox-9), and catabolic biomarkers related to osteoarthritis, such as cyclooxygenase-2 (Cox-2), matrix metalloproteinase-13 (Mmp13), and nuclear factor kappa-light-chain-enhancer of activated B cells (Nfκb), were evaluated by quantitative reverse transcriptase polymerase chain reaction and reporter gene assay...
December 2016: Preventive Nutrition and Food Science
https://www.readbyqxmd.com/read/28076568/immunohistochemical-analysis-of-retinoblastoma-cell-phenotype-using-neuronal-and-glial-cell-markers
#4
María Eugenia Orellana, Rubens Belfort, Emilia Antecka, Miguel Noel Burnier
Purpose: The cellular origin of retinoblastoma is uncertain as constituent tumor cells heterogeneously express markers of both immature and mature retinal cells. An immunohistochemical analysis of cellular origin may yield valuable insights into disease progression and treatment options. This study aimed to determine the cellular origin of retinoblastoma in a large case series and correlate these findings with histopathological prognostic factors. Methods: Thirty-nine retinoblastoma cases were histopathologically diagnosed and analyzed by immunohistochemistry using monoclonal antibodies against the immature neural cell marker SRY-box containing gene 2 (SOX-2), the mature neuronal cell marker microtubule-associated protein 2 (MAP2), and the mature glial cell marker glial fibrillary acidic protein (GFAP)...
November 2016: Arquivos Brasileiros de Oftalmologia
https://www.readbyqxmd.com/read/28052658/laminin-modulates-the-stem-cell-population-in-lm05-e-murine-breast-cancer-cells-through-the-activation-of-the-mapk-erk-pathway
#5
Damián E Berardi, Diego Raffo, Laura B Todaro, Marina Simian
Purpose: We investigated the effects of laminin on the fraction of cells with self-renewing capacity in the estrogen-dependent, tamoxifen-sensitive LM05-E breast cancer cell line. We also determined whether laminin affected the response to tamoxifen. Materials and Methods: The LM05-E breast cancer cell line was used as a model for all experiments. Aldehyde dehydrogenase (ALDH) activity, clonogenic and mammosphere assays were performed to measure the effects of laminin on modulation of the stem cell subpopulation...
December 6, 2016: Cancer Research and Treatment: Official Journal of Korean Cancer Association
https://www.readbyqxmd.com/read/28004397/general-health-influences-episodes-of-xerostomia-a-prospective-population-based-study
#6
Luciana da Silva, Emil Kupek, Karen G Peres
OBJECTIVES: The aim of this study was to investigate the associated factors of changes in symptoms of xerostomia (SOX) in adults aged 20-59. METHODS: A prospective population-based study was conducted in 2009 (n = 1720) and 2012 (n = 1222) in the urban area of Florianópolis, SC, Brazil. Information on SOX was collected in both years together with age, family income, years of schooling, smoking habit, alcohol consumption, changes in the body mass index (BMI; kg/m²), medicine use, self-reported diagnosis of chronic diseases, change in hypertension status and in the use and need for dentures, and number of remaining teeth...
December 22, 2016: Community Dentistry and Oral Epidemiology
https://www.readbyqxmd.com/read/27995033/extremely-high-expression-of-serum-alpha-fetoprotein-level-of-gastric-adenocarcinoma-a-rare-case-with-an-unexpected-well-prognosis
#7
Weihua Gong, Dawei Shou, Ping Gong
INTRODUCTION: Alpha-fetoprotein (AFP)-producing gastric cancer is a relatively rare form of stomach malignancy. Patients with higher serum AFP level (>300 ng/mL) will have a poorer prognosis. CASE DESCRIPTION: In this study, we present a case of 77-year-old woman with an extremely high expression of serum AFP level (>10,000 ng/mL) of gastric cancer. Histological examination revealed gastric adenocarcinoma (Bormann III gastric tumor) invading into the serosal layer and vessel...
2016: SpringerPlus
https://www.readbyqxmd.com/read/27994628/the-extract-of-fructus-psoraleae-promotes-viability-and-cartilaginous-formation-of-rat-chondrocytes-in-vitro
#8
Xin Pan, Kang Xu, Xuefeng Qiu, Wen Zhao, Dong Wang, Li Yang
This study aimed to investigate the extract components of FP on rat chondrocyte function and cartilaginous formation in vitro. Petroleum ether extract (P-e) of FP extract components was selected to treat Sprague-Dawley rat chondrocytes. Cell viability was tested with different concentrations (0.1, 1, 10, and 100 μg/mL) of P-e treatment. Concentrations of 0.1 and 1 μg/mL P-e conditioned culture mediums were used for treating chondrocytes in experiments. Cell proliferation was measured via DNA incorporation assay...
2016: Evidence-based Complementary and Alternative Medicine: ECAM
https://www.readbyqxmd.com/read/27990704/differentiation-of-bone-marrow-derived-stage-specific-embryonic-antigen-1-positive-pluripotent-stem-cells-into-male-germ-cells
#9
Reza Shirazi, Amir Hassan Zarnani, Masoud Soleimani, Karim Nayernia, Iraj Ragerdi Kashani
Studies published in recent years have changed the outlook on sterility and germ cell development by producing gametes from stem cells. In present study, a novel approach on differentiation of bone marrow-derived stage-specific embryonic antigen 1 positive (SSEA-1(+) ) pluripotent stem cells into male germ cells has been addressed. SSEA-1(+) stem cells were separated from murine bone marrow using magnetic-activated cell sorting (MACS) system and propagated on a feeder layer cells. To evaluate the pluripotency characteristic of the purified cells, they were differentiated toward cells of three germ layers...
December 19, 2016: Microscopy Research and Technique
https://www.readbyqxmd.com/read/27960359/noncompetitive-and-competitive-adsorption-of-heavy-metals-in-sulfur-functionalized-ordered-mesoporous-carbon
#10
Dipendu Saha, Soukaina Barakat, Scott E Van Bramer, Karl A Nelson, Dale K Hensley, Jihua Chen
In this work, sulfur-functionalized ordered mesoporous carbons were synthesized by activating the soft-templated mesoporous carbons with sulfur bearing salts that simultaneously enhanced the surface area and introduced sulfur functionalities onto the parent carbon surface. XPS analysis showed that sulfur content within the mesoporous carbons were between 8.2% and 12.9%. The sulfur functionalities include C-S, C═S, -COS, and SOx. SEM images confirmed the ordered mesoporosity within the material. The BET surface areas of the sulfur-functionalized ordered mesoporous carbons range from 837 to 2865 m(2)/g with total pore volume of 0...
December 14, 2016: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/27956231/viability-of-cells-from-displaced-fragments-of-the-elbow-osteochondritis-dissecans-alternative-source-of-autologous-chondrocyte-implantation
#11
Yusuke Kameda, Tadanao Funakoshi, Shunsuke Takeuchi, Katsura Sugawara, Norimasa Iwasaki
PURPOSE: To assess the histological properties of cells from displaced fragments obtained from patients with advanced osteochondritis dissecans (OCD) of the elbow and to examine whether these displaced fragments could be used as cell sources for autologous chondrocyte implantation. METHODS: We harvested 6 displaced fragments from 6 patients who underwent osteochondral mosaicplasty for OCD of the elbow. The displaced fragments were examined histologically and digested to obtain chondrocytes...
December 9, 2016: Arthroscopy: the Journal of Arthroscopic & related Surgery
https://www.readbyqxmd.com/read/27929467/an-optimized-protocol-for-electrophoretic-mobility-shift-assay-using-infrared-fluorescent-dye-labeled-oligonucleotides
#12
Yi-Wen Hsieh, Amel Alqadah, Chiou-Fen Chuang
Electrophoretic Mobility Shift Assays (EMSA) are an instrumental tool to characterize the interactions between proteins and their target DNA sequences. Radioactivity has been the predominant method of DNA labeling in EMSAs. However, recent advances in fluorescent dyes and scanning methods have prompted the use of fluorescent tagging of DNA as an alternative to radioactivity for the advantages of easy handling, saving time, reducing cost, and improving safety. We have recently used fluorescent EMSA (fEMSA) to successfully address an important biological question...
November 29, 2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/27925309/bovine-ovarian-stem-cells-differentiate-into-germ-cells-and-oocyte-like-structures-after-culture-in-vitro
#13
G B de Souza, Jjn Costa, E V da Cunha, Jrs Passos, R P Ribeiro, Mva Saraiva, R van den Hurk, Jrv Silva
Stem cells have been isolated from ovaries, and their ability to differentiate into oocytes in vitro has been demonstrated for mice and human, but not for bovine species. The aims of this study were to isolate germline stem cells from bovine ovaries and to evaluate the effects of bone morphogenetic proteins (BMPs) 2 and 4, and follicular fluid on the differentiation of these stem cells into oocyte-like structures. The ovarian stem cells were isolated and cultured in α-MEM(+) supplemented with BMP2, BMP4 or follicular fluid...
December 7, 2016: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/27923596/revision-hip-arthroplasty-using-a-modular-cementless-femoral-stem-intermediate-term-follow-up
#14
Sureshan Sivananthan, Chin-Tat Lim, Rapeepat Narkbunnam, Alex Sox-Harris, James I Huddleston, Stuart B Goodman
BACKGROUND: Modular femoral stem provides flexibility in femoral reconstruction, ensuring improved "fit and fill". However, there are risks of junction failure and corrosion, as well as cost concerns in the use of modular femoral stems. METHODS: We reviewed prospectively-gathered clinical and radiographic data on revision total hip arthroplasties (THAs) performed from 2001-2007 using modular, cementless femoral component performed by the 2 senior authors. Patients with a minimum follow-up of 7 years were included in this study...
November 2, 2016: Journal of Arthroplasty
https://www.readbyqxmd.com/read/27917009/induced-expression-of-cancer-stem-cell-markers-aldh1a3-and-sox-2-in-hierarchical-reconstitution-of-apoptosis-resistant-human-breast-cancer-cells
#15
Karin Kashii-Magaribuchi, Rie Takeuchi, Yuko Haisa, Akemi Sakamoto, Aimi Itoh, Yuki Izawa, Miyuki Isa, Mayu Fukuzawa, Motonobu Murakami, Rei Takahashi
We established an experimental system that can induce p53-dependent apoptosis by doxycycline treatment to analyze characteristics of the apoptosis-resistant cancer cell subpopulation in the human breast cancer cell line HCC1937. Expression patterns of the stem cell markers, ALDH1A3 and Sox-2, the luminal differentiation marker, GATA3 and the proliferation index marker, Ki-67 were analyzed using immunostaining and fluorescence-activated cell sorting (FACS). After doxycycline treatment, the number of viable cells was gradually decreased over seven days in a time-dependent manner due to p53-induced apoptosis; however, the number of smaller-sized ALDH1A3(+) cells assessed by immunostaining increased sharply after 1 day of doxycycline treatment, suggesting their apoptosis-resistant nature...
November 1, 2016: Acta Histochemica et Cytochemica
https://www.readbyqxmd.com/read/27911869/paclitaxel-oxaliplatin-fluorouracil-tof-regimen-versus-s-1-oxaliplatin-sox-regimen-for-metastatic-gastric-cancer-patients
#16
Xichao Dai, Xizhi Zhang, Chaomin Wang, Jingting Jiang, Changping Wu
AIMS AND BACKGROUND: This study was designed to compare the efficacy and safety of paclitaxel/oxaliplatin/fluorouracil (TOF) regimen and S-1/oxaliplatin (SOX) regimen for metastatic gastric cancer (GC) patients. METHODS: Sixty patients were divided into TOF group and SOX groups randomly. Patients in the TOF group received paclitaxel (135 mg/m2 iv) on day 1, oxaliplatin (100 mg/m2 iv) on day 1, fluorouracil (500 mg/m2 continuous iv) on day 1-5. The patients in the SOX group received oxaliplatin (130 mg/m2 iv) on day 1 and S-1 (40 mg~60mg orally twice/day based on body surface area) on days 1-14...
November 30, 2016: Oncotarget
https://www.readbyqxmd.com/read/27900891/chondrogenesis-of-human-adipose-derived-stem-cells-for-future-microtia-repair-using-co-culture-technique
#17
Bee See Goh, Siti Nurhadis Che Omar, Muhammad Azhan Ubaidah, Lokman Saim, Shamsul Sulaiman, Kien Hui Chua
CONCLUSION: In conclusion, these result showed HADSCs could differentiate into chondrocytes-like cells, dependent on signaling induced by TGF-β3 and chondrocytes. This is a promising result and showed that HADSCs is a potential source for future microtia repair. The technique of co-culture is a positive way forward to assist the microtia tissue. OBJECTIVE: Reconstructive surgery for the repair of microtia still remains the greatest challenge among the surgeons...
November 30, 2016: Acta Oto-laryngologica
https://www.readbyqxmd.com/read/27895125/lineage-specific-soxr-mediated-regulation-of-an-endoribonuclease-protects-non-enteric-bacteria-from-redox-active-compounds
#18
Jisun Kim, Chulwoo Park, James A Imlay, Woojun Park
Bacteria use redox-sensitive transcription factors to coordinate responses to redox stress. The [2Fe-2S] cluster-containing transcription factor SoxR is particularly tuned to protect cells against redox-active compounds (RACs). In enteric bacteria, SoxR is paired with a second transcription factor, SoxS, that activates downstream effectors. However, SoxS is absent in non-enteric bacteria, raising questions as to how SoxR functions. Here, we first show that SoxR of Acinetobacter oleivorans displayed similar activation profiles in response to RACs as did its homolog from Escherichia coli but controlled a different set of target genes, including sinE, which encodes an endoribonuclease...
January 6, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27863298/butein-inhibits-il-1%C3%AE-induced-inflammatory-response-in-human-osteoarthritis-chondrocytes-and-slows-the-progression-of-osteoarthritis-in-mice
#19
Wenhao Zheng, Hui Zhang, Yonglong Jin, Quan Wang, Linwei Chen, Zhenhua Feng, Hua Chen, Yaosen Wu
Osteoarthritis (OA) is a progressive degenerative disease characterized by irreversible articular cartilage destruction. Butein, a polyphenolic compound isolated from the stem bark of cashews and Rhus verniciflua Stokes, has been reported to have anti-inflammatory effects. This study aimed to assess the effect of butein on human OA chondrocytes and mice OA models induced by destabilization of the medial meniscus (DMM). In vitro, human OA chondrocytes were pretreated with butein at 10, 50μM and subsequently stimulated with IL-1β (10ng/ml) for 24h...
January 2017: International Immunopharmacology
https://www.readbyqxmd.com/read/27856123/prognostic-impact-of-primary-tumor-location-on-clinical-outcomes-of-metastatic-colorectal-cancer-treated-with-cetuximab-plus-oxaliplatin-based-chemotherapy-a-subgroup-analysis-of-the-jaccro-cc-05-06-trials
#20
Yu Sunakawa, Wataru Ichikawa, Akihito Tsuji, Tadamichi Denda, Yoshihiko Segawa, Yuji Negoro, Ken Shimada, Mitsugu Kochi, Masato Nakamura, Masahito Kotaka, Hiroaki Tanioka, Akinori Takagane, Satoshi Tani, Tatsuro Yamaguchi, Takanori Watanabe, Masahiro Takeuchi, Masashi Fujii, Toshifusa Nakajima
INTRODUCTION: Primary tumor location is a critical prognostic factor in metastatic colorectal cancer (mCRC); however, it remains unclear whether tumor location is a predictor of the response to cetuximab treatment. It is also uncertain if BRAF mutation contributes to the impact of tumor location on survival. We assessed the prognostic impact of tumor location on clinical outcomes in mCRC patients treated with first-line cetuximab chemotherapy. PATIENTS AND METHODS: The associations of tumor location with overall survival and progression-free survival were evaluated in mCRC patients with KRAS exon 2 wild-type tumors who were enrolled onto 2 clinical trials: JACCRO CC-05 of cetuximab plus FOLFOX (n = 57, UMIN000004197) and CC-06 of cetuximab plus SOX (n = 61, UMIN000007022)...
October 6, 2016: Clinical Colorectal Cancer
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