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https://www.readbyqxmd.com/read/28545668/phenethyl-isothiocyanate-suppresses-cancer-stem-cell-properties-in-vitro-and-in-a-xenograft-model
#1
Ji Ho Yun, Kyung-A Kim, Gyhye Yoo, Sun Young Kim, Ji Min Shin, Jung Hoon Kim, Sang Hoon Jung, Jungho Kim, Chu Won Nho
BACKGROUND: Cancer stem cells (CSCs) are a subset of cells within the bulk of a tumor that have the ability to self-renew and differentiate, and are thus associated with cancer invasion, metastasis, and recurrence. Phenethyl isothiocyanate (PEITC) is a natural compound found in cruciferous vegetables such as broccoli and is used as a cancer chemopreventive agent; however, its effects on CSCs are little known. PURPOSE: To evaluate the effect of PEITC on CSCs in this study by examining CSC properties...
July 1, 2017: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
https://www.readbyqxmd.com/read/28535666/ddx53-regulates-cancer-stem-cell-like-properties-by-binding-to-sox-2
#2
Youngmi Kim, Minjeong Yeon, Dooil Jeoung
This study investigated the role of cancer/testis antigen DDX53 in regulating cancer stem cell-like properties. DDX53 shows co-expression with CD133, a marker for cancer stem cells. DDX53 directly regulates the SOX-2 expression in anticancer drug-resistant Malme3MR cells. DDX53 and miR-200bwere found to be involved in the regulation of tumor spheroid forming potential of Malme3M and Malme3MR cells. Furthermore, the self-renewal activity and the tumorigenic potential of Malme3M(R)-CD133 (+) cells were also regulated by DDX53...
May 2, 2017: Molecules and Cells
https://www.readbyqxmd.com/read/28529185/care-2013-explanations-and-elaborations-reporting-guidelines-for-case-reports
#3
David S Riley, Melissa S Barber, Gunver S Kienle, Jeffry Aronson, Tido von Schoen-Angerer, Peter Tugwell, Helmut Kiene, Mark Helfand, Douglas G Altman, Harold Sox, Paul G Werthmann, David Moher, Richard A Rison, Larissa Shamseer, Christian A Koch, Gordon H Sun, Patrick Hanaway, Cleveland Clinic, Nancy L Sudak, Marietta Kaszkin-Bettag, Joel J Gagnier
Well-written and transparent case reports (1) reveal early signals of potential benefits, harms, and information on the use of resources; (2) provide information for clinical research and clinical practice guidelines (CPGs), and (3) inform medical education. High-quality case reports are more likely when authors follow reporting guidelines. During 2011-2012 a group of clinicians, researchers, and journal editors developed recommendations for the accurate reporting of information in case reports that resulted in the CARE (CAse REport) Statement and Checklist...
May 18, 2017: Journal of Clinical Epidemiology
https://www.readbyqxmd.com/read/28522297/three-dimensional-spheroid-culture-promotes-the-stemness-maintenance-of-cranial-stem-cells-by-activating-pi3k-akt-and-suppressing-nf-%C3%AE%C2%BAb-pathways
#4
Da He, Ren-Xian Wang, Jian-Ping Mao, Bin Xiao, Da-Fu Chen, Wei Tian
Multipotent stem cells are one of the most powerful tools available for the bone regeneration. However, owing to various limitations, including a lack of tissue-specific stem cell identification, reconstruction of large cranial bone defects remains challenging. In the current study, we isolated a population of Sca-1(+)CD105(+)CD140a(+) stem cells from adult mouse calvarium and cultured them as three-dimensional spheroids. Although these cells shared similar surface antigens when grown in either monolayers or spheroids, the cranial stem cells grown in spheroids possessed enhanced multipotency and proliferation capacity...
May 15, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28495880/sox4-allows-facultative-%C3%AE-cell-proliferation-through-repression-of-cdkn1a
#5
Eric E Xu, Shugo Sasaki, Thilo Speckmann, Cuilan Nian, Francis C Lynn
The HMG-box transcription factor, SOX4, is the most highly expressed SOX family protein in pancreatic islets, and mutations in Sox4 are associated with an increased risk of developing type 2 diabetes. We utilized an inducible β-cell knockout mouse model to test the hypothesis that Sox4 is essential for the maintenance of β-cell number during the development of type 2 diabetes. Knockout of Sox4 at 6 weeks of age resulted in time-dependent worsening of glucose tolerance, impairment of insulin secretion, and diabetes by 30 weeks of age...
May 11, 2017: Diabetes
https://www.readbyqxmd.com/read/28485205/effect-of-epigallocatechin-3-gallate-on-the-increase-in-type-ii-collagen-accumulation-in-cartilage-like-msc-sheets
#6
Keigo Sato, Hisashi Mera, Shigeyuki Wakitani, Mutsumi Takagi
With the aim to increase type II collagen content in the scaffold-free cartilage-like cell sheet using human bone marrow mesenchymal stem cells, we examined the effect of epigallocatechin-3-gallate (EGCG) addition to the chondrogenic medium for the cell sheet culture. The addition of EGCG (10 μM) increased the content of type II collagen 2-fold, while the addition did not markedly change the expression level of the genes encoding type II collagen and Sox 9. The reactive oxygen species level in the cells in cell sheets was thought to be too low to suppress the accumulation of type II collagen...
June 2017: Bioscience, Biotechnology, and Biochemistry
https://www.readbyqxmd.com/read/28484208/an-injectable-non-cross-linked-hyaluronic-acid-gel-containing-therapeutic-spheroids-of-human-adipose-derived-stem-cells
#7
Jingwei Feng, Kazuhide Mineda, Szu-Hsien Wu, Takanobu Mashiko, Kentaro Doi, Shinichiro Kuno, Kahori Kinoshita, Koji Kanayama, Rintaro Asahi, Ataru Sunaga, Kotaro Yoshimura
For chronic wounds, the delivery of stem cells in spheroidal structures can enhance graft survival and stem cell potency. We describe an easy method for the 3D culture of adipose-derived stem/stromal cells (ASCs) to prepare a ready-to-use injectable. We transferred suspensions of monolayer-cultured ASCs to a syringe containing hyaluronic acid (HA) gel, and then incubated the syringe as a 3D culture vessel. Spheroids of cells formed after 12 h. We found that 6 × 10(6) ASCs/ml in 3% HA gel achieved the highest spheroid density with appropriate spheroid sizes (20-100 µm)...
May 8, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28467624/palladium-catalyzed-cascade-sp-2-c-h-functionalization-intramolecular-asymmetric-allylation-from-aryl-ureas-and-1-3-dienes-to-chiral-indolines
#8
Shu-Sen Chen, Min-Song Wu, Zhi-Yong Han
A chiral Pd(II) -catalyzed cascade sp(2) C-H functionalization/intramolecular asymmetric allylation reaction is reported. A new chiral sulfoxide-oxazoline (SOX) ligand bearing single chiral center on the sulfur was identified as the optimal ligand for the reaction, being efficient both in the C-H cleavage step and the stereocontrol of the allylation step. The broad scope of this method with respect to aryl ureas and 1,3-dienes enables the rapid construction of valuable chiral indoline derivatives with high yields and enantioselectivities (up to 99 % yield, up to 95:5 e...
June 1, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28461325/role-of-rac1-pathway-in-epithelial-to-mesenchymal-transition-and-cancer-stem-like-cell-phenotypes-in-gastric-adenocarcinoma
#9
Changhwan Yoon, Soo-Jeong Cho, Kevin K Chang, Do Joong Park, Sandra Ryeom, Sam S Yoon
Rac1, a Rho GTPase family member, is dysregulated in a variety of tumor types including gastric adenocarcinoma (GA), but little is known about its role in cancer stem-like cells (CSCs). Therefore, Rac1 activity and inhibition were examined in GA cells and mouse xenograft models for epithelial-to-mesenchymal transition (EMT) and CSC phenotypes. Rac1 activity was significantly higher in spheroid-forming or CD44(+) GA CSCs compared to unselected cells. Rac1 inhibition using Rac1 shRNA or a Rac1 inhibitor (NSC23766) decreased expression of the self-renewal transcription factor, Sox-2, decreased spheroid formation by 78-81%, and prevented tumor initiation in immunodeficient mice...
May 1, 2017: Molecular Cancer Research: MCR
https://www.readbyqxmd.com/read/28440454/microrna%C3%A2-336-directly-targets-sox%C3%A2-2-in-osteosarcoma-to-inhibit-tumorigenesis
#10
Yong Cao, Tianding Wu, Dongzhe Li, Jianzhong Hu, Hongbin Lu
Previous evidence has suggested that microRNAs (miRNAs or miRs), which belong to a class of non‑coding RNAs, shape cellular processes by regulating gene expression. Abnormal expression of miRNAs has been associated with tumorigenesis in multiple cancers. However, the function of miR‑336 in osteosarcoma (OS) remains unknown. The experimental procedures used in the present study included flow cytometry, reverse transcription‑quantitative polymerase chain reaction, luciferase reporter assay, invasion assay, western blot analysis and in vivo implantation...
June 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28429362/wnt11-overexpression-promote-adipose-derived-stem-cells-differentiating-to-the-nucleus-pulposus-like-phenotype
#11
H-T Chen, A-B Huang, Y-L He, J Bian, H-J Li
OBJECTIVE: Our present study aimed to evaluate the effects of Wnt11 overexpression on the adipose-derived stem (ADSCs) cells differentiation to the nucleus pulposus (NP) cells and its function in the ADSCs cells growth, proliferation and induction of the NP cells markers. MATERIALS AND METHODS: The cell growth was detected using the (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) (MTT) assay and the cell cycle was assessed by the flow cytometry...
April 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28424427/nos2-expression-in-glioma-cell-lines-and-glioma-primary-cell-cultures-correlation-with-neurosphere-generation-and-sox-2-expression
#12
Paola Palumbo, Gianfranca Miconi, Benedetta Cinque, Francesca Lombardi, Cristina La Torre, Soheila Raysi Dehcordi, Renato Galzio, Annamaria Cimini, Antonio Giordano, Maria Grazia Cifone
Nitric oxide has been implicated in biology and progression of glioblastoma (GBM) being able to influence the cellular signal depending on the concentration and duration of cell exposure. NOS2 (inducible nitric oxide synthase) have been proposed as a component of molecular profile of several tumors, including glioma, one of the most aggressive primary brain tumor featuring local cancer stem cells responsible for enhanced resistance to therapies and for tumor recurrence. Here, we investigated the NOS2 mRNA expression by reverse transcription-PCR in human glioma primary cultures at several grade of malignancy and glioma stem cell (GSC) derived neurospheres...
April 11, 2017: Oncotarget
https://www.readbyqxmd.com/read/28395800/derivation-and-characterization-of-the-human-embryonic-stem-cell-line-cr-4-differentiation-to-human-retinal-pigment-epithelial-cells
#13
John L Mazzilli, Aleksey Y Domozhirov, Stacey L Mueller-Ortiz, Charles A Garcia, Rick A Wetsel, Eva M Zsigmond
The CR-4 human embryonic stem cell line was derived from the inner cell mass of a developing blastocyst. This cell line has been adapted to grow in feeder-free conditions and is especially well-suited for differentiation to retinal pigment epithelium. The line demonstrates a normal human 46,XX female karyotype. Pluripotency was assessed through qRT-PCR for expression of NANOG, OCT-4, and SOX-2. A teratoma assay was performed and results were positive for all three germ layers. Testing for Mycoplasma was negative...
January 2017: Stem Cell Research
https://www.readbyqxmd.com/read/28389060/paraneoplastic-limbic-encephalitis-with-sox1-and-pca2-antibodies-and-relapsing-neurological-symptoms-in-an-adolescent-with-hodgkin-lymphoma
#14
M Kunstreich, J H Kreth, P T Oommen, J Schaper, M Karenfort, O Aktas, D Tibussek, F Distelmaier, A Borkhardt, M Kuhlen
BACKGROUND: Immune cross-reactivity between malignant and normal tissues causes the rare, so called paraneoplastic syndrome (PS). In approximately 60% of the patients, various onconeural antibodies are detectable in the cerebrospinal fluid (CSF) and are associated with typical tumour entities. METHODS: We report an unusual case of paraneoplastic limbic encephalitis (PLE) in a 17-year-old adolescent with classical Hodgkin lymphoma. RESULTS: He presented with a variety of neurologic and neuropsychiatric symptoms, profound B-symptoms and typical MRI findings including hyperintense lesions with contrast enhancement in the medial temporal lobe and limbic system...
March 27, 2017: European Journal of Paediatric Neurology: EJPN
https://www.readbyqxmd.com/read/28375818/chondrocyte-attachment-proliferation-and-differentiation-on-three-dimensional-polycaprolactone-fumarate-scaffolds
#15
Eric R Wagner, Joshua Parry, Mahrokh Dadsetan, Dalibel Bravo, Scott M Riester, Andre J van Wijnen, Michael J Yaszemski, Sanjeev Kakar
Current treatment options for cartilage injuries are limited. The goals of this study are to create a biodegradable polymer scaffold with the capabilities of sustaining chondrocyte growth and proliferation, enable cell-to-cell communication and tissue regeneration through large pores, and assess the biological augmentation of the scaffold capabilities using platelet lysate (PL). We synthesized biodegradable polycaprolactone fumarate (PCLF) scaffolds to allow cell-cell communication through large interconnected pores...
March 31, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28373426/actinomycin-d-down-regulates-sox2-expression-and-induces-death-in-breast-cancer-stem-cells
#16
Tuhin Das, Rajesh R Nair, Ryan Green, Shruti Padhee, Mark Howell, Jit Banerjee, Shyam S Mohapatra, Subhra Mohapatra
BACKGROUND/AIM: One of the major hurdles in the treatment of breast cancers is the inability of anti-cancer drugs to eliminate the breast cancer stem cells (BCSCs) population, which leads to disease relapse. The dearth in anti-cancer drugs that target BCSCs can be attributed to the absence of in vitro screening models that can not only recapitulate the tumor microenvironment consisting of BCSCs but also preserve the 3-dimensional (3D) architecture of in vivo tumors. MATERIALS AND METHODS: In our present study, we have developed a 3D cell culture system that shows: (i) enrichment of BCSCs, (ii) increased drug resistance, and (iii) generation of hypoxic conditions similar to tumors...
April 2017: Anticancer Research
https://www.readbyqxmd.com/read/28373172/mouse-embryonic-dorsal-root-ganglia-contain-pluripotent-stem-cells-that-show-features-similar-to-embryonic-stem-cells-and-induced-pluripotent-stem-cells
#17
Ryuhei Ogawa, Kyohei Fujita, Kazuo Ito
In the present study, we showed that the dorsal root ganglion (DRG) in the mouse embryo contains pluripotent stem cells (PSCs) that have developmental capacities equivalent to those of embryonic stem (ES) cells and induced pluripotent stem cells. Mouse embryonic DRG cells expressed pluripotency-related transcription factors [octamer-binding transcription factor 4, SRY (sex determining region Y)-box containing gene (Sox) 2, and Nanog] that play essential roles in maintaining the pluripotency of ES cells. Furthermore, the DRG cells differentiated into ectoderm-, mesoderm- and endoderm-derived cells...
May 15, 2017: Biology Open
https://www.readbyqxmd.com/read/28358903/clusters-of-amniotic-fluid-cells-and-their-associated-early-neuroepithelial-markers-in-experimental-myelomeningocele-correlation-with-astrogliosis
#18
Jolanta Zieba, Amanda Miller, Oleg Gordiienko, George M Smith, Barbara Krynska
Myelomeningocele (MMC) is the most common and severe disabling type of spina bifida resulting in the exposure of vulnerable spinal cord to the hostile intrauterine environment. In this study, we sought to examine the cellular content of fetal amniotic fluid (AF) in MMC and explore a correlation between these cells and pathological development of MMC. MMC was induced in fetal rats by exposing pregnant mothers to all-trans retinoic acid and AF samples were collected before term. Cells were isolated from AF samples and morphologically and phenotypically characterized in short-term cultures...
2017: PloS One
https://www.readbyqxmd.com/read/28334711/quantitative-proteomics-analysis-of-ischemia-reperfusion-injury-modulated-proteins-in-cardiac-microvascular-endothelial-cells-and-the-protective-role-of-tongxinluo
#19
Qing Li, He-He Cui, Yue-Jin Yang, Xiang-Dong Li, Gui-Hao Chen, Xia-Qiu Tian, Chen Jin, Qiu-Ting Dong, Pei-Sen Huang, Jun Xu
BACKGROUND: The protection of endothelial cells (ECs) against reperfusion injury has received little attention. In this study, we used Tandem Mass Tag (TMT) labeling proteomics to investigate the modulated proteins in an in vitro model of cardiac microvascular endothelial cells (CMECs) subjected to ischemia/reperfusion (I/R) injury and their alteration by traditional Chinese medicine Tongxinluo (TXL). METHODS: Human CMECs were subjected to 2 h of hypoxia followed by 2 h of reoxygenation with different concentrations of TXL Protein expression profiles of CMECs were determined using tandem mass spectrometry...
2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28322869/scaffold-free-high-throughput-generation-of-quiescent-valvular-microtissues
#20
Annelies Roosens, Inès Puype, Ria Cornelissen
AIMS: The major challenge of working with valvular interstitial cells in vitro is the preservation or recovery of their native quiescent state. In this study, a biomimetic approach is used which aims to engineer small volume, high quality valve microtissues, having a potential in regenerative medicine and as a relevant 3D in vitro model to provide insights into valve (patho)biology. METHODS AND RESULTS: To form micro-aggregates, porcine valvular interstitial cells were seeded in agarose micro-wells and cultured in medium supplemented with 250μM Ascorbic Acid 2-phosphate for 22days...
March 18, 2017: Journal of Molecular and Cellular Cardiology
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