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https://www.readbyqxmd.com/read/28718680/development-of-emu-oil-loaded-pcl-collagen-bioactive-nanofibers-for-proliferation-and-stemness-preservation-of-human-adipose-derived-stem-cell-possible-application-in-regenerative-medicine
#1
Kazem Nejati-Koshki, Younes Pilehvar-Soltanahmadi, Effat Alizadeh, Abbas Ebrahimi-Kalan, Yousef Mortazavi, Nosratollah Zarghami
Adipose tissue-derived stem cells (ASCs) are promising candidate in stem cell therapies, and maintaining their stemness potential is vital to achieve effective treatment. Natural based scaffolds have been recently attracted increasing attention in nanomedicine and drug delivery. In the present study, a polymeric nanofibrous scaffold was developed based on the polycaprolactone/Collagen (PCL/Coll) containing Emu oil as a bioactive material to induce the proliferation of ASCs, while simultaneously preserve the stemness property of those cells...
July 18, 2017: Drug Development and Industrial Pharmacy
https://www.readbyqxmd.com/read/28718379/expression-of-embryonic-stem-cell-markers-in-acute-myeloid-leukemia
#2
Tiphanie Picot, Carmen Mariana Aanei, Amandine Fayard, Pascale Flandrin-Gresta, Sylvie Tondeur, Marina Gouttenoire, Emmanuelle Tavernier-Tardy, Eric Wattel, Denis Guyotat, Lydia Campos
Acute myeloid leukemia is driven by leukemic stem cells which can be identified by cross lineage expression or arrest of differentiation compared to normal hematopoietic stem cells. Self-renewal and lack of differentiation are also features of stem cells and have been associated with the expression of embryonic genes. The aim of our study was to evaluate the expression of embryonic antigens (OCT4, NANOG, SOX2, SSEA1, SSEA3) in hematopoietic stem cell subsets (CD34(+)CD38(-) and CD34(+)CD38(+)) from normal bone marrows and in samples from acute myeloid leukemia patients...
July 2017: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
https://www.readbyqxmd.com/read/28716899/hdac1-upregulation-by-nanog-promotes-multidrug-resistance-and-a-stem-like-phenotype-in-immune-edited-tumor-cells
#3
Kwon-Ho Song, Chel Hun Choi, Hyo-Jung Lee, Se Jin Oh, Seon Rang Woo, Soon-Oh Hong, Kyung Hee Noh, Hanbyoul Cho, Eun Joo Chung, Jae-Hoon Kim, Joon-Yong Chung, Stephen M Hewitt, Seungki Baek, Kyung-Mi Lee, Cassian Yee, Minjoo Son, Chih-Ping Mao, T-C Wu, Tae Woo Kim
Cancer immunoediting drives the adaptation of tumor cells to host immune surveillance. Immunoediting driven by antigen (Ag)-specific T cells enriches NANOG expression in tumor cells, resulting in a stem-like phenotype and immune resistance. Here we identify HDAC1 as a key mediator of the NANOG-associated phenotype. NANOG upregulated HDAC1 through promoter occupancy, thereby decreasing histone H3 acetylation on K14 and K27. NANOG-dependent, HDAC1-driven epigenetic silencing of cell cycle inhibitor CDKN2D and CDKN1B induced stem-like features...
July 17, 2017: Cancer Research
https://www.readbyqxmd.com/read/28716052/%C3%AE-arrestin1-mediated-acetylation-of-gli1-regulates-hedgehog-gli-signaling-and-modulates-self-renewal-of-shh-medulloblastoma-cancer-stem-cells
#4
Evelina Miele, Agnese Po, Federica Begalli, Laura Antonucci, Angela Mastronuzzi, Carlo Efisio Marras, Andrea Carai, Danilo Cucchi, Luana Abballe, Zein Mersini Besharat, Giuseppina Catanzaro, Paola Infante, Lucia Di Marcotullio, Gianluca Canettieri, Enrico De Smaele, Isabella Screpanti, Franco Locatelli, Elisabetta Ferretti
BACKGROUND: Aberrant Sonic Hedgehog/Gli (Hh/Gli) signaling pathway is a critical regulator of Sonic hedgehog medulloblastoma (SHH-MB). Cancer stem cells (CSCs), thought to be largely responsible for tumor initiation, maintenance, dissemination and relapse, have been identified in SHH-MB. Since we previously demonstrated that Hh/Gli signaling controls CSCs features in SHH-MB and that in these tumors miR-326 is down regulated, here we investigated whether there is a functional link between Hh/Gli signaling and miR-326...
July 17, 2017: BMC Cancer
https://www.readbyqxmd.com/read/28714175/schwann-cells-secrete-extracellular-vesicles-to-promote-and-maintain-the-proliferation-and-multipotency-of-hdpcs
#5
Ziyue Li, Yan Liang, Kuangwu Pan, Hui Li, Mei Yu, Weihua Guo, Guoqing Chen, Weidong Tian
OBJECTIVES: Schwann cells (SCs) are the principal glial cells in peripheral nerve system, involved in neuropathies with great regenerative potential. Dental pulp cells have been reported to maintain neurogenic potential. In contrast, the regulatory role of SCs on human dental pulp cells (hDPCs) development remains undefined. MATERIALS AND METHODS: SC secretion and SC-derived extracellular vesicles (EVs) were collected and used to treat hDPCs; and proliferation and multiple differentiation of hDPCs were detected after EVs treatments...
August 2017: Cell Proliferation
https://www.readbyqxmd.com/read/28712374/parthenogenetic-activation-and-somatic-cell-nuclear-transfer-of-porcine-oocytes-activated-by-an-electric-pulse-and-azd5438-treatment
#6
Xiao-Chen Li, Qing Guo, Hai-Ying Zhu, Long Jin, Yu-Chen Zhang, Guang-Lei Zhang, Xiao-Xu Xing, Mei-Fu Xuan, Qi-Rong Luo, Zhao-Bo Luo, Jun-Xia Wang, Cheng-Du Cui, Wen-Xue Li, Zheng-Yun Cui, Xi-Jun Yin, Jin-Dan Kang
We examined the in vitro developmental competence of parthenogenetic activation (PA) oocytes activated by an electric pulse (EP) and treated with various concentrations of AZD5438 for 4 h. Treatment with 10 µM AZD5438 for 4 h significantly improved the blastocyst formation rate of PA oocytes in comparison with 0, 20, or 50 µM AZD5438 treatment (46.4% vs. 34.5%, 32.3%, and 24.0%, respectively; P 0.05). Furthermore, 66.67% of blastocysts derived from these AZD5438-treated PA oocytes had a diploid karyotype...
July 17, 2017: Zygote: the Biology of Gametes and Early Embryos
https://www.readbyqxmd.com/read/28711945/the-molecular-characterization-of-porcine-egg-precursor-cells
#7
Te-Sha Tsai, Jacqueline Johnson, Yvonne White, Justin C St John
Female-factor infertility can be caused by poor oocyte quality and depleted ovarian reserves. Egg precursor cells (EPCs), isolated from the ovarian cortex, have the potential to be used to overcome female infertility. We aimed to define the origins of EPCs by analyzing their gene expression profiles and mtDNA content using a mini-pig model. We characterized FAC-sorted DDX4+-derived porcine EPCs by performing RNA-sequencing and determined that they utilize pathways important for cell cycle and proliferation, which supports the existence of adult mitotically active oogonial cells...
June 28, 2017: Oncotarget
https://www.readbyqxmd.com/read/28708036/amniotic-fluid-cells-show-higher-pluripotency-related-gene-expression-than-allantoic-fluid-cells
#8
Debora Kehl, Melanie Generali, Sabrina Görtz, Diego Geering, Jaroslav Slamecka, Simon P Hoerstrup, Ulrich Bleul, Benedikt Weber
Amniotic fluid represents an abundant source of multipotent stem cells, referred as broadly mul-tipotent given their differentiation potential and expression of pluripotency-related genes. How-ever, the origin of this broadly multipotent cellular fraction is not fully understood. Several sources have been proposed so far, including embryonic and extra-embryonic tissues. In this re-gard, the ovine developmental model uniquely allows for direct comparison of fetal fluid-derived cells from two separate fetal fluid cavities, the allantois and the amnion, over the entire duration of gestation...
July 14, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/28703793/linc-dync2h1-4-promotes-emt-and-csc-phenotypes-by-acting-as-a-sponge-of-mir-145-in-pancreatic-cancer-cells
#9
Yuran Gao, Zhicheng Zhang, Kai Li, Liying Gong, Qingzhu Yang, Xuemei Huang, Chengcheng Hong, Mingfeng Ding, Huanjie Yang
The acquisition of epithelial-mesenchymal transition (EMT) and/or existence of a sub-population of cancer stem-like cells (CSC) are associated with malignant behavior and chemoresistance. To identify which factor could promote EMT and CSC formation and uncover the mechanistic role of such factor is important for novel and targeted therapies. In the present study, we found that the long intergenic non-coding RNA linc-DYNC2H1-4 was upregulated in pancreatic cancer cell line BxPC-3-Gem with acquired gemcitabine resistance...
July 13, 2017: Cell Death & Disease
https://www.readbyqxmd.com/read/28701751/secretome-of-in-vitro-cultured-human-embryos-contains-extracellular-vesicles-that-are-uptaken-by-the-maternal-side
#10
Elisa Giacomini, Riccardo Vago, Ana Maria Sanchez, Paola Podini, Natasa Zarovni, Valentina Murdica, Roberta Rizzo, Daria Bortolotti, Massimo Candiani, Paola Viganò
Communication between embryo and maternal endometrium occurs during a specific time frame in which implantation is possible. Here we demonstrate for the first time that conditioned media from non-manipulated human embryos cultured in vitro for 3 days or up to the blastocyst stage contain extracellular vesicles (EVs) with a diameter of 50 to 200 nm and bearing the traditional microvesicle and exosome marker proteins CD63, CD9 and ALIX. The embryonic origin of these EVs has been confirmed by the presence of stemness gene transcripts and their enrichment in the non-classical HLA-G protein...
July 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28700586/systematic-identification-and-characterization-of-regulatory-elements-derived-from-human-endogenous-retroviruses
#11
Jumpei Ito, Ryota Sugimoto, Hirofumi Nakaoka, Shiro Yamada, Tetsuaki Kimura, Takahide Hayano, Ituro Inoue
Human endogenous retroviruses (HERVs) and other long terminal repeat (LTR)-type retrotransposons (HERV/LTRs) have regulatory elements that possibly influence the transcription of host genes. We systematically identified and characterized these regulatory elements based on publicly available datasets of ChIP-Seq of 97 transcription factors (TFs) provided by ENCODE and Roadmap Epigenomics projects. We determined transcription factor-binding sites (TFBSs) using the ChIP-Seq datasets and identified TFBSs observed on HERV/LTR sequences (HERV-TFBSs)...
July 12, 2017: PLoS Genetics
https://www.readbyqxmd.com/read/28699108/the-role-of-epithelial-mesenchymal-transition-in-squamous-cell-carcinoma-of-the-oral-cavity
#12
Nina Zidar, Emanuela Boštjančič, Marija Malgaj, Nina Gale, Tadej Dovšak, Vojko Didanovič
Epithelial-mesenchymal transition (EMT) has emerged as a possible mechanism of cancer metastasizing, but strong evidence for EMT involvement in human cancer is lacking. Our aim was to compare oral spindle cell carcinoma (SpCC) as an example of EMT with oral conventional squamous cell carcinoma (SCC) with and without nodal metastases to test the hypothesis that EMT contributes to metastasizing in oral SCC. Thirty cases of oral SCC with and without nodal metastasis and 15 cases of SpCC were included. Epithelial (cytokeratin, E-cadherin), mesenchymal (vimentin, N-cadherin), and stem cell markers (ALDH-1, CD44, Nanog, Sox-2) and transcription repressors (Snail, Slug, Twist) were analyzed immunohistochemically...
July 12, 2017: Virchows Archiv: An International Journal of Pathology
https://www.readbyqxmd.com/read/28693150/dhfr-and-mdr1-upregulation-is-associated-with-chemoresistance-in-osteosarcoma-stem-like-cells
#13
Yu-Hsien Lee, Hui-Wen Yang, Li-Chiu Yang, Ming-Yi Lu, Lo-Lin Tsai, Shun-Fa Yang, Yu-Feng Huang, Ming-Yung Chou, Cheng-Chia Yu, Fang-Wei Hu
Tumor-initiating cells (TICs) are defined as a specialized subset of cells with tumor-initiating capacity that can initiate tumor growth, tumor relapse and metastasis. In the present study, osteosarcoma TICs (OS-TICs) were isolated and enriched from the osteosarcoma U2OS and MG-63 cell lines using sphere formation assays and serum-depleted media. These enriched OS-TICs showed the expression of several typical cancer stemness markers, including octamer-binding transcription factor 4, Nanog homeobox, cluster of differentiation (CD)117, Nestin and CD133, and the expression of ATP binding cassette subfamily G member 2, multidrug resistance protein 1 (MDR1) and dihydrofolate reductase (DHFR)...
July 2017: Oncology Letters
https://www.readbyqxmd.com/read/28690187/evaluating-of-oct-4-and-nanog-was-differentially-regulated-by-a-new-derivative-indole-in-leukemia-cell-line
#14
Mojgan Noroozi Karimabad, Mehdi Mahmoodi, Abdollah Jafarzadeh, Ali Darehkordi, Mohammad Reza Hajizadeh, Hossein Khorramdelazad, Ahmad Reza Sayadi, Fariba Rahmani, Gholamhossein Hassanshahi
BACKGROUND: The potential exists to improve treatment through characterization of tumor stem cells and identification of therapeutic targets Using OCT-4 and NANOG genes. Here we have synthesized and investigated the potential of; New Indole-3-carbaldehyde derivative (NI-3-CD) in inhibiting the expression of self-renewal regulatory factors and cancer stem cell gene in a leukemia cell line NB4. METHODOLOGY: The NB4 cells were cultured in RPMI1640 medium contained NI-3-CD and I3F (15...
July 8, 2017: Immunology Letters
https://www.readbyqxmd.com/read/28685064/identification-of-rhodamine-123-positive-stem-cell-subpopulations-in-canine-hepatocellular-carcinoma-cells
#15
Harumichi Itoh, Shimpei Nishikawa, Tomoya Haraguchi, Yu Arikawa, Masato Hiyama, Toshie Iseri, Yoshiki Itoh, Munekazu Nakaichi, Yasuho Taura, Kenji Tani, Kazuhito Itamoto
The majority of cases of chemotherapy for hepatocellular carcinoma (HCC) are not effective in human or veterinary medicine due to resistance against anticancer agents. In human medicine, hepatocellular carcinoma stem cells (HCSCs) were recently identified as cytokeratin 19 (CK19)-, cluster of differentiation (CD)-44-, and CD133-positive. However, there are few previous reports regarding canine HCSC (cHCSC). Additionally, to the best of our knowledge, the chemoresistance against anticancer agents of these cHCSCs has not been investigated...
July 2017: Biomedical Reports
https://www.readbyqxmd.com/read/28684962/nanog-dynamics-in-mouse-embryonic-stem-cells-results-from-systems-biology-approaches
#16
REVIEW
Lucia Marucci
Mouse embryonic stem cells (mESCs), derived from the inner cell mass of the blastocyst, are pluripotent stem cells having self-renewal capability and the potential of differentiating into every cell type under the appropriate culture conditions. An increasing number of reports have been published to uncover the molecular mechanisms that orchestrate pluripotency and cell fate specification using combined computational and experimental methodologies. Here, we review recent systems biology approaches to describe the causes and functions of gene expression heterogeneity and complex temporal dynamics of pluripotency markers in mESCs under uniform culture conditions...
2017: Stem Cells International
https://www.readbyqxmd.com/read/28684898/autophagy-regulates-the-stemness-of-cervical-cancer-stem-cells
#17
Yi Yang, Li Yu, Jin Li, Ya Hong Yuan, Xiao Li Wang, Shi Rong Yan, Dong Sheng Li, Yan Ding
Cancer stem cells (CSCs) are a rare population of multipotent cells with the capacity to self-renew. It has been reported that there are CSCs in cervical cancer cells. Pluripotency-associated (PA) transcription factors such as Oct4, Sox2, Nanog and CD44 have been used to isolate CSCs subpopulations. In this study, we showed that autophagy plays an important role in the biological behavior of cervical cancer cells. The expression of the autophagy protein Beclin 1 and LC3B was higher in tumorspheres established from human cervical cancers cell lines (and CaSki) than in the parental adherent cells...
2017: Biologics: Targets & Therapy
https://www.readbyqxmd.com/read/28677539/an-integration-free-virus-free-rhesus-macaque-induced-pluripotent-stem-cell-line-ripsc90-from-embryonic-fibroblasts
#18
Enrique Sosa, Rachel Kim, Ernesto J Rojas, Linzi Hosohama, Jon D Hennebold, Kyle E Orwig, Amander T Clark
The rhesus macaque induced pluripotent stem cell (riPSC) line, UCLAi090-A (riPSC90), was generated from rhesus embryonic fibroblast (REF) cells called REF90. REF90 cells and the riPSC90 line were authenticated by short tandem repeat analysis and had a normal male (42, XY) karyotype. The riPSC90 line expressed markers of self-renewal including OCT4, NANOG, TRA-1-81 and SSEA4, and generated teratomas after transplantation into immunocompromised mice. riPSC90 could be used in parallel with riPSC89, which was derived from REFs cultured from a different rhesus macaque embryo (Sosa et al...
May 2017: Stem Cell Research
https://www.readbyqxmd.com/read/28677037/suppression-of-a-cancer-stem-like-phenotype-mediated-by-alpha-lipoic-acid-in-human-lung-cancer-cells-through-down-regulation-of-%C3%AE-catenin-and-oct-4
#19
Preeyaporn Plaimee Phiboonchaiyanan, Pithi Chanvorachote
PURPOSE: Cancer stem cells (CSCs) that possess the ability of self-renewal and multi-potency have been shown to drive tumor progression and metastasis. The majority of recent studies has focused on potential molecules targeting CSCs so as to develop novel strategies for efficient cancer treatment or protection. Here, we show how alpha-lipoic acid (LA), an endogenous mitochondrial anti-oxidant, affects the CSC-like phenotypes of human non-small cell lung cancer-derived H23, H292 and H460 cells...
July 4, 2017: Cellular Oncology (Dordrecht)
https://www.readbyqxmd.com/read/28676130/skeletal-muscle-derived-interstitial-progenitor-cells-pics-display-stem-cell-properties-being-clonogenic-self-renewing-and-multi-potent-in-vitro-and-in-vivo
#20
Beverley J Cottle, Fiona C Lewis, Victoria Shone, Georgina M Ellison-Hughes
BACKGROUND: The development of cellular therapies to treat muscle wastage with disease or age is paramount. Resident muscle satellite cells are not currently regarded as a viable cell source due to their limited migration and growth capability ex vivo. This study investigated the potential of muscle-derived PW1(+)/Pax7(-) interstitial progenitor cells (PICs) as a source of tissue-specific stem/progenitor cells with stem cell properties and multipotency. METHODS: Sca-1(+)/PW1(+) PICs were identified on tissue sections from hind limb muscle of 21-day-old mice, isolated by magnetic-activated cell sorting (MACS) technology and their phenotype and characteristics assessed over time in culture...
July 4, 2017: Stem Cell Research & Therapy
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