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https://www.readbyqxmd.com/read/29245050/nanog-restores-the-impaired-myogenic-differentiation-potential-of-skeletal-myoblasts-after-multiple-population-doublings
#1
Aref Shahini, Debanik Choudhury, Mohammadnabi Asmani, Ruogang Zhao, Pedro Lei, Stelios T Andreadis
Adult skeletal muscle regeneration relies on the activity of satellite cells residing in the skeletal muscle niche. However, systemic and intrinsic factors decrease the myogenic differentiation potential of satellite cells thereby impairing muscle regeneration. Here we present data showing that late passage C2C12 myoblasts exhibited significantly impaired myogenic differentiation potential that was accompanied by impaired expression of myogenic regulatory factors (Myf5, MyoD, Myogenin, and MRF4) and members of myocyte enhancer factor 2 family...
December 2, 2017: Stem Cell Research
https://www.readbyqxmd.com/read/29243844/in-vitro-improvement-of-quail-primordial-germ-cell-expansion-through-activation-of-tgf-beta-signaling-pathway
#2
Saeed Yakhkeshi, Shaban Rahimi, Mohsen Sharafi, Seyedeh-Nafiseh Hassani, Sara Taleahmad, Abdolhossein Shahverdi, Hossein Baharvand
Avian primordial germ cells (PGCs) have valuable potentials to cell-based approaches for transgenic bird production. In this regard, improvement of avian PGC expansion in vitro is necessary. Among experimental avian species, quail is a good model for transgenic technology, especially due to its short generation time. In the present study, we have examined the proliferative effects of transforming growth factor β (TGF-β) on the quail PGCs. After isolation of quail PGCs from blood [Hamburger-Hamilton (HH stages 13-15)] and gonads (HH stages 28-30), these cells were cultured on quail embryonic fibroblasts (QEF)...
December 15, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29243835/diallyl-trisulfide-inhibits-breast-cancer-stem-cells-via-suppression-of-wnt-%C3%AE-catenin-pathway
#3
Xiaoting Li, Yu Meng, Chunfeng Xie, Jianyun Zhu, Xiaoqian Wang, Yuan Li, Shanshan Geng, Jieshu Wu, Caiyun Zhong, Min Li
Cancer stem cells (CSCs) play a central role in the development of breast cancer. The canonical Wnt/β-catenin signal pathway is critical for maintaining CSCs characteristics. Diallyl trisulfide (DATS), a natural organosulfur compound from the garlic, exhibits effective antitumor properties. However, the role of DATS in regulating breast CSCs activity and the underlying molecular mechanisms remain obscure. In the present study, we reported that DATS efficiently inhibited the viability of breast CSCs as evidenced by reducing turmorspheres formation, decreasing the expression of breast CSCs markers (CD44, ALDH1A1, Nanog and Oct4), as well as inhibiting proliferation and inducing apoptosis...
December 15, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29243438/-effects-of-aqueous-extracts-of-gecko-on-stemness-of-hepatocellular-carcinoma
#4
Hui-Ling Huang, Ya-Lan Zhang, Wen-Fa Chen, Chun-Ling Wu
To explore the effects and mechanism of aqueous extracts of gecko on cancer stem cells properties of hepatocellular carcinoma. In vitro, MTT assay was used to detect the cells growth in Huh7 and Hep3B. Spheroid-forming assay and flow cytometry were performed to observe the the stemness of Huh7 and Hep3B cells. The protein expressions of β-catenin, CD44, c-Myc, CCND1, Sox2, Oct4, Nanog and ABCG2 were detected by Western blot. Interacting proteins were detected by co-immunoprecipitation; and a subcutaneous xenograft model was used to detect the stemness of hepatoma carcinoma cells...
October 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/29235536/genetically-unmatched-human-ipsc-and-esc-exhibit-equivalent-gene-expression-and-neuronal-differentiation-potential
#5
Hany E Marei, A Althani, S Lashen, C Cenciarelli, Anwarul Hasan
The potential uniformity between differentiation and therapeutic potential of human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) remains debatable. We studied the gene expression profiles, pathways analysis and the ability to differentiated into neural progenitor cells (NPCs) and motor neurons (MNs) of genetically unmatched integration-free hiPSC versus hESC to highlight possible differences/similarities between them at the molecular level. We also provided the functional information of the neurons derived from the different hESCs and hiPSCs lines using the Neural Muscular Junction (NMJ) Assay...
December 13, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29232016/characterization-of-h3-3-and-hira-expression-and-function-in-bovine-early-embryos
#6
Kun Zhang, Han Wang, Sandeep K Rajput, Joseph K Folger, George W Smith
Histone variant H3.3 is encoded by two distinct genes, H3F3A and H3F3B, that are closely associated with actively transcribed genes. H3.3 replacement is continuous and essential for maintaining correct chromatin structure during mouse oogenesis. Upon fertilization, H3.3 is incorporated to parental chromatin, and is required for blastocyst formation in mice. The H3.3 exchange process is facilitated by the chaperone HIRA, particularly during zygote development. We previously demonstrated that H3.3 is required for bovine early embryonic development; here, we explored the mechanisms of its functional requirement...
December 12, 2017: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/29229769/cytoplasmic-polyadenylation-mediated-translational-control-of-maternal-mrnas-directs-maternal-to-zygotic-transition
#7
Cecilia Lanny Winata, Maciej Łapiński, Leszek Pryszcz, Candida Vaz, Muhammad Hisyam Bin Ismail, Srikanth Nama, Hajira Shreen Hajan, Serene Gek Ping Lee, Vladimir Korzh, Prabha Sampath, Vivek Tanavde, Sinnakaruppan Mathavan
In the earliest stages of animal development following fertilization, maternally deposited mRNAs direct biological processes to the point of zygotic genome activation (ZGA). These maternal mRNAs undergo cytoplasmic polyadenylation (CPA), suggesting translational control of their activation. To elucidate the biological role of CPA during embryogenesis, we performed genome-wide polysome profiling at several stages of zebrafish development. Our analysis revealed a correlation between CPA and polysome association dynamics, demonstrating a coupling of translation to the CPA of maternal mRNAs...
December 11, 2017: Development
https://www.readbyqxmd.com/read/29228699/iron-depletion-is-a-novel-therapeutic-strategy-to-target-cancer-stem-cells
#8
Takayuki Ninomiya, Toshiaki Ohara, Kazuhiro Noma, Yuki Katsura, Ryoichi Katsube, Hajime Kashima, Takuya Kato, Yasuko Tomono, Hiroshi Tazawa, Shunsuke Kagawa, Yasuhiro Shirakawa, Fumiaki Kimura, Ling Chen, Tomonari Kasai, Masaharu Seno, Akihiro Matsukawa, Toshiyoshi Fujiwara
Adequate iron levels are essential for human health. However, iron overload can act as catalyst for the formation of free radicals, which may cause cancer. Cancer stem cells (CSCs), which maintain the hallmark stem cell characteristics of self-renewal and differentiation capacity, have been proposed as a driving force of tumorigenesis and metastases. In the present study, we investigated the role of iron in the proliferation and stemness of CSCs, using the miPS-LLCcm cell model. Although the anti-cancer agents fluorouracil and cisplatin suppressed the proliferation of miPS-LLCcm cells, these drugs did not alter the expression of stemness markers, including Nanog, SOX2, c-Myc, Oct3/4 and Klf4...
November 17, 2017: Oncotarget
https://www.readbyqxmd.com/read/29228234/tgfbr2-inactivation-facilitates-cellular-plasticity-and-development-of-pten-null-prostate-cancer
#9
Wei Zhao, Peng Tan, Qingyuan Zhu, Adebusola Ajibade, Teng Long, Wenyong Long, Qingtian Li, Pinghua Liu, Bo Ning, Helen Y Wang, Rong-Fu Wang
Mutations in tumors can create a state of increased cellular plasticity that promotes resistance to treatment. Thus, there is an urgent need to develop novel strategies for identifying key factors that regulate cellular plasticity in order to combat resistance to chemotherapy and radiation treatment. Here we report that prostate epithelial cell reprogramming could be exploited to identify key factors required for promoting prostate cancer tumorigenesis and cellular plasticity. Deletion of phosphatase and tensin homolog (Pten) and transforming growth factor-beta receptor type 2 (Tgfbr2) may increase prostate epithelial cell reprogramming efficiency in vitro and cause rapid tumor development and early mortality in vivo...
December 8, 2017: Journal of Molecular Cell Biology
https://www.readbyqxmd.com/read/29222037/diabetes-as-risk-factor-for-pancreatic-cancer-hyperglycemia-promotes-epithelial-mesenchymal-transition-and-stem-cell-properties-in-pancreatic-ductal-epithelial-cells
#10
Sascha Rahn, Vivien Zimmermann, Fabrice Viol, Hendrike Knaack, Kerstin Stemmer, Lena Peters, Lennart Lenk, Hendrik Ungefroren, Dieter Saur, Heiner Schäfer, Ole Helm, Susanne Sebens
Type 2 diabetes mellitus (T2DM) is associated with hyperglycemia and a risk to develop pancreatic ductal adenocarcinoma (PDAC), one of the most fatal malignancies. Cancer stem cells (CSC) are essential for initiation and maintenance of tumors, and acquisition of CSC-features is linked to epithelial-mesenchymal-transition (EMT). The present study investigated whether hyperglycemia promotes EMT and CSC-features in premalignant and malignant pancreatic ductal epithelial cells (PDEC). Under normoglycemia (5 mM d-glucose), Panc1 PDAC cells but not premalignant H6c7-kras cells exhibited a mesenchymal phenotype along with pronounced colony formation...
December 5, 2017: Cancer Letters
https://www.readbyqxmd.com/read/29216888/telomere-heterogeneity-linked-to-metabolism-and-pluripotency-state-revealed-by-simultaneous-analysis-of-telomere-length-and-rna-seq-in-the-same-human-embryonic-stem-cell
#11
Hua Wang, Kunshan Zhang, Yifei Liu, Yudong Fu, Shan Gao, Peng Gong, Haiying Wang, Zhongcheng Zhou, Ming Zeng, Zhenfeng Wu, Yu Sun, Tong Chen, Siguang Li, Lin Liu
BACKGROUND: Telomere length heterogeneity has been detected in various cell types, including stem cells and cancer cells. Cell heterogeneity in pluripotent stem cells, such as embryonic stem cells (ESCs), is of particular interest; however, the implication and mechanisms underlying the heterogeneity remain to be understood. Single-cell analysis technology has recently been developed and effectively employed to investigate cell heterogeneity. Yet, methods that can simultaneously measure telomere length and analyze the global transcriptome in the same cell have not been available until now...
December 8, 2017: BMC Biology
https://www.readbyqxmd.com/read/29215942/cocktail-of-chemical-compounds-and-recombinant-proteins-robustly-promote-the-stemness-of-adipose-derived-stem-cells
#12
Yonglong Guo, Quan Yu, Sanjana Mathew, Ruiling Lian, Yunxia Xue, Zekai Cui, Shanyi Li, Deliang Zhu, Yuting Han, Qiaolang Zeng, Shiwei Liu, Jiansu Chen
Induced pluripotent stem cells (iPSCs) from somatic cells can be reprogrammed to provide an unlimited cell resource showing great potential in disease modeling and regenerative medicine. However, the traditional method for reprogramming cells into iPSCs using genome-integrating retro- or lenti-viruses remain an obstacle for its application in clinical settings. We tried the possibility to generate pre-iPSCs from human adipose-derived stem cells (ADSCs) by nongenetic reprogramming using recombinant cell-penetrating proteins OCT4/KLF4/SOX2 (PTD-OKS) and the cocktail of small molecules (VCFZ)...
December 2017: Cellular Reprogramming
https://www.readbyqxmd.com/read/29214996/the-role-of-cdx2-as-a-lineage-specific-transcriptional-repressor-for-pluripotent-network-during-the-first-developmental-cell-lineage-segregation
#13
Daosheng Huang, Guoji Guo, Ping Yuan, Amy Ralston, Lingang Sun, Mikael Huss, Tapan Mistri, Luca Pinello, Huck Hui Ng, Guocheng Yuan, Junfeng Ji, Janet Rossant, Paul Robson, Xiaoping Han
The first cellular differentiation event in mouse development leads to the formation of the blastocyst consisting of the inner cell mass (ICM) and trophectoderm (TE). The transcription factor CDX2 is required for proper TE specification, where it promotes expression of TE genes, and represses expression of Pou5f1 (OCT4). However its downstream network in the developing embryo is not fully characterized. Here, we performed high-throughput single embryo qPCR analysis in Cdx2 null embryos to identify CDX2-regulated targets in vivo...
December 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29214774/correlation-of-cancer-stem-cell-markers-oct4-sox2-and-nanog-with-clinicopathological-features-and-prognosis-in-operative-patients-with-rectal-cancer
#14
Liuping You, Xin Guo, Yuenan Huang
PURPOSE: To investigate the association of cancer stem-cell markers [octamer-binding transcription factor 4 (OCT4), sex determining region Y-box 2 (SOX2), and Nanog homebox (NANOG)] expression with clinicopathological properties and overall survival (OS) in operative rectal cancer (RC) patients receiving adjuvant therapy. MATERIALS AND METHODS: 153 patients with primary RC receiving surgery were enrolled. Tumor tissue and paired adjacent normal tissue sample were collected, and OCT4, SOX2, and NANOG expressions were assessed by immunofluorescent staining...
January 2018: Yonsei Medical Journal
https://www.readbyqxmd.com/read/29212799/lif-activated-jak-signaling-determines-esrrb-expression-during-late-stage-reprogramming
#15
Delun Huang, Ling Wang, Jingyue Duan, Chang Huang, Xiuchun Cindy Tian, Ming Zhang, Young Tang
The regulatory process of naïve-state induced pluripotent stem cells (iPSCs) generation is not well understood. Leukemia inhibitory factor (LIF) activated Janus kinase/signal transducer and activator of transcription 3 (Jak/Stat3) is the master regulator for naïve-state pluripotency achievement and maintenance. The estrogen-related receptor beta (Esrrb) serves as a naïve-state marker gene regulating self-renewal of embryonic stem cells (ESCs). However, the inter-connection between Esrrb and LIF signaling for pluripotency establishment in reprogramming is unclear...
December 6, 2017: Biology Open
https://www.readbyqxmd.com/read/29205617/epigenetic-states-of-donor-cells-significantly-affect-the-development-of-somatic-cell-nuclear-transfer-scnt-embryos-in-pigs
#16
Yanhui Zhai, Wei Li, Zhiren Zhang, Yunqing Cao, Zhengzhu Wang, Sheng Zhang, Ziyi Li
The type and pattern of epigenetic modification in donor cells can significantly affect the developmental competency of somatic cell nuclear transfer (SCNT) embryos. Here, we investigated the developmental capacity, gene expression, and epigenetic modifications of SCNT embryos derived from porcine bone marrow-derived mesenchymal stem cells (BMSCs) and fetal fibroblasts (FFs) donor cells compared to embryos obtained from in vitro fertilization (IVF). Compared to FFs, the donor BMSCs had more active epigenetic markers (Histone H3 modifications: H3K9Ac, H3K4me3, and H3K4me2) and fewer repressive epigenetic markers (H3K9me3, H3K9me2, and DNA methyltransferase 1)...
December 4, 2017: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/29205098/platelet-derived-growth-factor-receptor-alpha-expressing-cardiac-progenitor-cells-can-be-derived-from-previously-cryopreserved-human-heart-samples
#17
Thi Yen Loan Le, Hilda Pickett, Cristobal Dos Remedios, Pasquale Barbaro, Eddy Kizana, James Chong
Cardiac progenitor cells (CPCs) are being developed as a promising treatment for heart failure. Although clinical trials have predominantly used donor cardiac biopsies to derive CPCs, a better solution could be to use previously cryopreserved human heart tissue. This would enable timely and convenient access to healthy and young heart samples for CPC production. However, few studies have attempted to isolate CPCs from previously cryopreserved heart tissue. In this study, we isolated CPCs from eight non-diseased human heart samples previously cryopreserved as part of the Sydney Heart Bank...
December 5, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/29204746/comparative-analysis-of-human-wharton-s-jelly-mesenchymal-stem-cells-derived-from-different-parts-of-the-same-umbilical-cord
#18
Dinesh Bharti, Sharath Belame Shivakumar, Ji-Kwon Park, Imran Ullah, Raghavendra Baregundi Subbarao, Ji-Sung Park, Sung-Lim Lee, Bong-Wook Park, Gyu-Jin Rho
Easy isolation, lack of ethical issues, high proliferation, multi-lineage differentiation potential and immunomodulatory properties of umbilical cord (UC)-derived mesenchymal stem cells (MSCs) make them a valuable tool in stem cell research. Recently, Wharton's jelly (WJ) was proven as the best MSC source among various compartments of UC. However, it is still unclear whether or not Wharton's jelly-derived MSCs (WJMSCs) from different parts of the whole cord exhibit the same characteristics. There may be varied MSCs present in different parts of WJ throughout the length of the UC...
December 4, 2017: Cell and Tissue Research
https://www.readbyqxmd.com/read/29199459/isolation-and-characterization-of-mesenchymal-stem-cells-and-its-antitumor-application-on-ovarian-cancer-cell-line
#19
Farideh Mohammadian, Babak Negahdari
The molecular interaction network of Oct-4 (POU5F1) and NANOG connected to regulation and growth of mesenchymal stem cells (MSCs) were supplemented with information of miRNA to find an important micro-RNAs and supplemented molecular interaction network. Following co-culturing of Bone marrow mesenchymal stem cells (BMMSCs) with SKOV3 ovarian cancer cell lines and undifferentiated BMMSCs, MTT was analyzed for cell cytotoxicity. The analyses of the expression of miRNA were performed either after oesteogenesis (hsa-miR-34 and hsa-miR-335) or chondrogenic (hsa-miR-145 and hsa-miR-455) differentiation...
December 3, 2017: Artificial Cells, Nanomedicine, and Biotechnology
https://www.readbyqxmd.com/read/29198990/prognostic-and-clinicopathological-value-of-nanog-in-hepatocellular-carcinoma-a-meta-analysis
#20
REVIEW
Chaojie Liang, Kaitong Zhang, Hua Ge, Wei Li, Guangming Li, Jixiang Wu
BACKGROUND AND AIMS: Recently, studies indicate that Nanog is over-expressed in hepatocellular carcinoma (HCC); however, the relationship between Nanog expression and clinicopathological and prognostic value remains controversial. Therefore, we conducted a meta-analysis to explore the role of Nanog in HCC. METHODS: Articles were included from PubMed, Cochrane Library, Web of Science, EMBASE database, Chinese CNKI, and the Chinese WanFang database. The relationships between Nanog expression, clinicopathological features, and survival rate were calculated...
December 1, 2017: Clinica Chimica Acta; International Journal of Clinical Chemistry
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