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H9c2 and extract

Cheng Zheng, Jia-Feng Lin, Zhong-Hao Lin, Wei-Qian Lin, Saroj Thapa, Yuan-Zheng Lin, Hao Lian, Zhi-Rui Liu, Jia-Hui Chen, Xiao-Wei Li
With the chronic ischemia persisting after acute myocardial infarction, the accompanying low-degree inflammation and subsequent fibrosis result in progression of cardiac remodeling and heart failure. Recently, Sodium Houttuyfonate (SH), a pure compound extracted from Houttuynia cordata , has been confirmed exerting anti-inflammatory and anti-fibrotic effects under diseased situations. Here, we aimed to investigate whether SH could reverse the cardiac remodeling post-myocardial infarction by alleviating cardiac inflammation and fibrosis...
2018: Frontiers in Pharmacology
Jing Xie, Jing Xiong, Li-Sheng Ding, Li Chen, Hua Zhou, Liang Liu, Zhi-Feng Zhang, Xue-Mei Hu, Pei Luo, Lin-Sen Qing
Although herb medicines have become the major source for new drug discovery, many of them are largely under-explored due to the purity-activity relationship. Efficient identification of bioactive compounds in conventional stepwise separation and isolation has not yet been elucidated. Therefore, we proposed a new separation strategy for holism understanding of herb pharmacology using molecularly imprinted polymers (MIPs). Astragali Radix (AR), important in traditional Chinese medicine, was chosen in this study for multicomponent knockout followed by bioactivity evaluation...
September 17, 2018: Journal of Chromatography. A
Donghai Chu, Zhenqiu Zhang
Trichosanthis Pericarpium (TP) is a traditional Chinese medicine for treating cardiovascular diseases. In this study, we investigated the effects of TP aqueous extract (TPAE) on hypoxia/reoxygenation (H/R) induced injury in H9c2 cardiomyocytes and explored the underlying mechanisms. H9c2 cells were cultured under the hypoxia condition induced by sodium hydrosulfite for 30 min and reoxygenated for 4 h. Cell viability was measured by MTT assay. The amounts of LDH, NO, eNOS, and iNOS were tested by ELISA kits...
September 20, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Vincenzo Quagliariello, Raffaele Vecchione, Carmela Coppola, Chiara Di Cicco, Alberta De Capua, Giovanna Piscopo, Rolando Paciello, Viviana Narciso, Carmen Formisano, Orazio Taglialatela-Scafati, Rosario Vincenzo Iaffaioli, Gerardo Botti, Paolo Antonio Netti, Nicola Maurea
Doxorubicin is a highly active antineoplastic agent, but its clinical use is limited because of its cardiotoxicity. Although nutraceuticals endowed with anti-inflammatory properties exert cardioprotective activity, their bioavailability and stability are inconsistent. In an attempt to address this issue, we evaluated whether bioavailable nanoemulsions loaded with nutraceuticals (curcumin and fresh and dry tomato extracts rich in lycopene) protect cardiomyoblasts (H9C2 cells) from doxorubicin-induced toxicity...
September 14, 2018: Nutrients
Wei Zhao, Yongwei Wang, Guanshan Wei, Shiyuan Xu
OBJECTIVE: To investigate the role of poly(ADP-ribose) polymerases-1 (PARP-1)-mediated blockade of autophagic flow in myocardial ischemia-reperfusion injury. METHODS: H9c2 cells, a rat cardiac myocyte line, were divided into control group, hypoxia/ reoxygenation model group (H/R group), PARP-1 inhibitor (PJ34) group, and PJ34 + H/R group. The total protein was extracted from the cells in each group to detect the expressions of pADPr, Bax, the DNA damage marker protein p-YH2ax, and autophagic flow-associated proteins LC3BⅡ/LC3Ⅰ, Beclin-1, and P62 using Western blotting...
July 30, 2018: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
Dong Kwon Yang
Oxidative stress plays an important role in the progression of cardiac diseases, including ischemia/reperfusion injury, myocardial infarction, and heart failure. Growing evidence indicates that cabbage has various pharmacological properties against a wide range of diseases, such as cardiovascular diseases, hepatic diseases, and cancer. However, little is known about its effects on oxidative stress in cardiomyocytes or the underlying mechanisms. Therefore, the present study examined the effects of cabbage extract on oxidative stress in H9c2 cardiomyoblasts...
2018: Evidence-based Complementary and Alternative Medicine: ECAM
Yao Pan, Ze-Yuan Deng, Shi-Lian Zheng, Xuan Chen, Bing Zhang, Hongyan Li
The hydrophilic extracts of mulberry (HEM) and blueberry (HEB) and lipophilic extracts of mango (LEM) and watermelon (LEW) were mixed in different ratios to assess the antioxidant interactions by chemical-based (DPPH and ABTS assays) and H9c2 cell-based models. There were both synergistic and antagonistic antioxidant interactions among these fruits. Some groups with combinational extracts showed stronger synergistic antioxidant effects than the individual groups, and others (HEM-LEW F1/10, LEW-LEM F5/10, and HEB-LEM F3/10) showed stronger antagonistic effects than the individual groups based on the indicators [the values of DPPH, ABTS, and MTT; the expression of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT), and malondialdehyde (MDA); the release of lactate dehydrogenase (LDH); and the quantification of cellular antioxidant activity]...
August 29, 2018: Journal of Agricultural and Food Chemistry
Yousef Faridvand, Samira Nozari, Simin Atashkhoei, Mohammad Nouri, Ahmadreza Jodati
Protection of the cardiac cell against hypoxia-induced cell damage is one of the main approaches to preventing cardiovascular disease. Earlier studies have shown the cardioprotective effect of the human Amniotic Membrane (hAM) in animal model of cardiac injury. However, the effect of Amniotic Membrane Proteins (AMPs), extracted from hAM, on myocardial hypoxia injury remains unclear. So, our study aimed to investigate the protective effect of AMPs against hypoxia-induced cardiomyocytes apoptosis. H9c2 cardiomyocytes were pre-treated with AMPs followed by 24 h in hypoxia condition...
September 10, 2018: Biochemical and Biophysical Research Communications
Simei Sun, Tianyi Li, Li Jin, Zhe Hao Piao, Bin Liu, Yuhee Ryu, Sin Young Choi, Gwi Ran Kim, Ji Eun Jeong, An Jin Wi, Song Ju Lee, Hae Jin Kee, Myung Ho Jeong
An extract of Dendropanax morbifera branch exerts antioxidant, anti-inflammatory, antithrombotic, and anticancer activities. The purpose of this study was to investigate the effect of the extract in isoproterenol-induced cardiac hypertrophy. Phalloidin staining showed that treatment with the extract dramatically prevents isoproterenol-induced H9c2 cell enlargement and the expression of cardiac hypertrophic marker genes, including atrial natriuretic peptide (ANP) and B-type brain natriuretic peptide (BNP). Further, pretreatment with the extract decreased isoproterenol-induced GATA4 and Sp1 expression in H9c2 cells...
2018: American Journal of Chinese Medicine
Jui-Ting Chiang, Khan Farheen Badrealam, Marthandam Asokan Shibu, Chia-Hua Kuo, Chih-Yang Huang, Bih-Cheng Chen, Yueh-Min Lin, Vijaya Padma Viswanadha, Wei-Wen Kuo, Chih-Yang Huang
Eriobotrya japonica (EJ) is a traditional Chinese plant with high medicinal value. EJ extracts are reported to exhibit antioxidant and anti-inflammatory biological attributes. The current study aims to evaluate the prospective efficacy of E. japonica leave extract (EJLE) against Angiotensin-II induced cardiac hypertrophy in H9c2 cardiomyoblast and in spontaneously hypertensive rats (SHRs). For the in vitro studies, Angiotensin-II pretreated H9c2 cells were treated with EJLE and analyzed through Western blotting and rhodamine phalloidin staining for their cardio-protective attributes...
July 4, 2018: Environmental Toxicology
Yi-Han Zuo, Quan-Bin Han, Geng-Ting Dong, Rui-Qi Yue, Xue-Cong Ren, Jian-Xin Liu, Liang Liu, Pei Luo, Hua Zhou
Background and Objective: Ischemic heart disease (IHD) has been the major issue of public health. Panax ginseng (ginseng) has been verified as an effective traditional Chinese medicines and exerted cardioprotective effect. This study aimed to investigate the polysaccharide fraction of ginseng on hypoxia/reoxygenation (H/R) injury in cardiomyocytes and the underlying mechanisms. Methods: Ginseng was extracted by ethanol and fractionated by high-speed counter current chromatography (HSCCC) and column separation...
2018: Frontiers in Physiology
Yao Pan, Hongyan Li, Shilian Zheng, Bing Zhang, Ze-Yuan Deng
The hydrophilic extracts of eggplant peel (HEEP) and purple sweet potato (HEPP) and lipophilic extracts of tomato (LET) and carrot (LEC) were mixed in different ratios to assess the significance of the compatibility of aliments, based on their antioxidant and anti-inflammatory interactions in H9c2 cells. The results indicated that groups of some combinational extracts (HEPP-HEEP F1/10, LEC-HEEP F3/10, LEC-HEPP F3/10) showed stronger synergistic antioxidant and anti-inflammatory effects than individual groups...
July 18, 2018: Journal of Agricultural and Food Chemistry
Yun Huang, Kenneth Kin Leung Kwan, Ka Wing Leung, Huaiyou Wang, Xiang Peng Kong, Tina Ting Xia Dong, Karl Wah Keung Tsim
Astragali Radix (AR) is a widely used "Qi-invigorating" herb in China for its tonic effects in strengthening biological tissues. The extract of AR contains abundant antioxidants, including astragalosides and isoflavonoids. However, very few reports have systematically measured the effects of the major components of AR on cell mitochondrial bioenergetics. Here, a systemic approach employing an extracellular flux analyzer was developed to evaluate mitochondrial respiration in cultured cardiomyocyte cells H9C2...
May 25, 2018: International Journal of Molecular Sciences
Li-Xing Wu, Yu-Yu Xu, Zhi-Jian Yang, Qing Feng
Myocardial infarction triggers massive biochemical changes, even cardiac cell death. Endoplasmic reticulum stress is involved in the pathology of myocardial infarction-mediated apoptosis. In the present study, myocardial cell line H9c2 cells were treated with cobalt chloride (CoCl2 ) to induce hypoxia. Isoproterenol was used for two successive days to induce myocardial infarction in SD rats. The cardioprotective effect of olive leaf extract (OLE) and its main constituent hydroxytyrosol and the underlying mechanisms were evaluated...
September 29, 2018: Journal of Biomedical Research
Xiang Zheng, Shushu Wang, Dakai Chen, Xiaoming Yang
Swainsonine is an Astragalus membranaceus extract. It is indole, alkaloid, and soluble in water. Its effect on rat cardiomyocytes apoptosis, and the mechanisms underlying that effect, were investigated by inducing apoptosis in H9c2 cells. This was detected by MTT assay, Annexin V-FITC/propidium iodide double staining and western blotting. Flow cytometry and fluorescence microscopy were used to confirm swainsonine's effect on mitochondrial membrane potential and levels of reactive oxygen species, while an ATP-dependent bioluminescence assay kit served to find the ATP contents...
April 30, 2018: Cellular and Molecular Biology
Xu Yin, Feng Zhao, Xiao Feng, Junjun Li, Xinyao Yang, Hexin'ge Zhang, Pengfei Tu, Xingyun Chai
Encouraged by the anti-myocardial ischemic effect of Corydalis hendersonii ethanol extract, a chemical reinvestigation of the whole plant of C. hendersonii was performed, which led to the isolation of four new spirobenzylisoquinoline N-oxide alkaloids, hendersines C-F (1-4), along with seven known isoquinoline alkaloids (5-11). The structures of the new isolates including absolute configurations were elucidated by the analysis of spectroscopic data and comparison of the experimental and calculated electronic circular dichroism (ECD) data...
July 2018: Fitoterapia
Ya He, Chen Li, Qiaoya Ma, Songsheng Chen
Esculetin (6,7-dihydroxycoumarin), a natural coumarin compound extracted from natural plants, was reported to be involved in ischemia/reperfusion (I/R) injury. However, the role of esculetin in myocardial I/R injury remains unclear. This study was designed to investigate the protective effects of esculetin on cardiomyocytes induced by hypoxia/reoxygenation (H/R), and explore the underlying mechanisms. Our results showed that esculetin improved the cell viability and decreased lactate dehydrogenase (LDH) release in H/R-stimulated H9c2 cells...
June 18, 2018: Biochemical and Biophysical Research Communications
Mohan Manu T, T Anand, Farhath Khanum
Cardiovascular diseases are one of the major global health issues leading to morbidity and mortality across the world. In the present study Bacopa monniera and its major bioactive component, Bacoside A (Bac-A) was used to evaluate its cytoprotective property in H9C2 cardiomyocytes against tBHP (150 μM) induced ROS-mediated oxidative stress and apoptosis. Our results implicate that pre-treatment with hydroalcoholic extract of Bacopa monniera (BME) and Bac-A (125 μg/ml and 6 μg/ml respectively) significantly restored oxidative stress by scavenging the free radicals and also elevated phase II antioxidant defensive enzymes such as (SOD, CAT, GR, GPx and GSH)...
June 2018: Pathophysiology: the Official Journal of the International Society for Pathophysiology
Qiang Su, Junjing Yao, Cunjian Sheng
Myocarditis is a cardiomyopathy associated with inflammatory response. It has been reported that geniposide (GEN), a traditional Chinese herb extract from Gardenia jasminoides Ellis, possesses an anti-inflammatory effect and a protective effect on cardiomyocytes. The present study aimed to explore the protective role of GEN and the underlying mechanism in LPS-injured H9c2 cells. H9c2 cells were treated with LPS to induce cell injury and then we investigated the effect of GEN. miR-145 expression was inhibited by transfection with miR-145 inhibitor and its expression was measured by RT-PCR...
August 2018: Inflammation
Xian Wen Tan, Mrinal Bhave, Alan Yean Yip Fong, Eiji Matsuura, Kazuko Kobayashi, Lian Hua Shen, Siaw San Hwang
[This corrects the article DOI: 10.1155/2016/6943053.].
2018: Oxidative Medicine and Cellular Longevity
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