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Cardiomyocytes and extract

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https://www.readbyqxmd.com/read/30308230/meroterpenoids-from-ganoderma-sinense-protect-hepatocytes-and-cardiomyocytes-from-oxidative-stress-induced-injuries
#1
Suyu Gao, Peng Zhang, Cuiyun Zhang, Fengyan Bao, Hua Li, Lixia Chen
Four meroterpenoids, applanatumols F (1), H (3), I (2), and lingzhiol (4) were isolated from the 95% EtOH extract of the fruiting bodies of Ganoderma sinense. Their structures were established on the basis of NMR spectroscopic analyses, optical rotatory dispersion data, ECD spectra, and X-ray crystallography. Compounds 1, 2, 4 existed as racemic mixtures ((+) 1a, 2a, 4a; (-) 1b, 2b, 4b), while 3 as a single enantiomer. Base on the seperated enantiomers, we sought to explicit possible effects of compounds 1-4 on hydrogen peroxide (H2 O2 )-induced cell death and to determine their underlying molecular mechanisms in human normal liver LO2 cells...
October 8, 2018: Fitoterapia
https://www.readbyqxmd.com/read/30278018/label-free-identification-of-antibody-mediated-rejection-in-cardiac-allograft-biopsies-using-infrared-spectroscopic-imaging
#2
Imran Uraizee, Vishal K Varma, Hari Sreedhar, Francesca Gambacorta, Shaiju Nazeer, Aliya Husain, Michael J Walsh
BACKGROUND: Antibody-mediated rejection (AMR) in cardiac allograft recipients remains less well-understood than acute cellular rejection, is associated with worse outcomes, and portends a greater risk of developing chronic allograft vasculopathy. Diffuse immunohistochemical (IHC) C4d staining of capillary endothelia in formalin-fixed, paraffin-embedded (FFPE) right ventricular (RV) endomyocardial biopsies is diagnostic of immunopathologic AMR but serves more as a late-stage marker. Infrared (IR) spectroscopy may be a useful tool in earlier detection of rejection...
October 1, 2018: Transplantation
https://www.readbyqxmd.com/read/30256522/gentisic-acid-attenuates-pressure-overload-induced-cardiac-hypertrophy-and-fibrosis-in-mice-through-inhibition-of-the-erk1-2-pathway
#3
Simei Sun, Hae Jin Kee, Li Jin, Yuhee Ryu, Sin Young Choi, Gwi Ran Kim, Myung Ho Jeong
We previously reported that gentisic acid (2,5-dihydroxybenzoic acid) is the third most abundant phenolic component of Dendropanax morbifera branch extracts. Here, we investigated its effects on cardiac hypertrophy and fibrosis in a mouse model of pressure overload and compared them to those of the beta blocker bisoprolol and calcium channel blocker diltiazem. Cardiac hypertrophy was induced in mice by transverse aortic constriction (TAC). Beginning 2 weeks after this procedure, the mice were given daily intraperitoneal injections of gentisic acid (100 mg/kg/d), bisoprolol (5 mg/kg/d) or diltiazem (10 mg/kg/d) for 3 weeks...
September 6, 2018: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/30241309/trichosanthis-pericarpium-aqueous-extract-protects-h9c2-cardiomyocytes-from-hypoxia-reoxygenation-injury-by-regulating-pi3k-akt-no-pathway
#4
Donghai Chu, Zhenqiu Zhang
Trichosanthis Pericarpium (TP) is a traditional Chinese medicine for treating cardiovascular diseases. In this study, we investigated the effects of TP aqueous extract (TPAE) on hypoxia/reoxygenation (H/R) induced injury in H9c2 cardiomyocytes and explored the underlying mechanisms. H9c2 cells were cultured under the hypoxia condition induced by sodium hydrosulfite for 30 min and reoxygenated for 4 h. Cell viability was measured by MTT assay. The amounts of LDH, NO, eNOS, and iNOS were tested by ELISA kits...
September 20, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/30199522/the-effects-of-dracocephalum-heterophyllum-benth-flavonoid-on-hypertrophic-cardiomyocytes-induced-by-angiotensin-ii-in-rats
#5
Hong Jiang, Chen Zhang, Wen He
BACKGROUND Dracocephalum heterophyllum Benth flavonoid (DHBF) is a Tibetan and Uighur traditional medicine used to treat various disorders such as hypertension, lung heat, cough, and bronchitis; it has good antioxidant activity. Previous studies have shown that DHBF can reduce blood pressure in renovascular hypertensive rats, improve left ventricular systolic and diastolic function, and improve myocardial contractility. Therefore, we aimed to study the effect of DHBF on cardiomyocyte hypertrophy in cultured cells...
September 10, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/30187876/-role-of-poly-adp-ribose-polymerases-1-mediated-blockade-of-autophagy-in-ischemia-reperfusion-injury-of-rat-cardiomyocytes
#6
Wei Zhao, Yongwei Wang, Guanshan Wei, Shiyuan Xu
OBJECTIVE: To investigate the role of poly(ADP-ribose) polymerases-1 (PARP-1)-mediated blockade of autophagic flow in myocardial ischemia-reperfusion injury. METHODS: H9c2 cells, a rat cardiac myocyte line, were divided into control group, hypoxia/ reoxygenation model group (H/R group), PARP-1 inhibitor (PJ34) group, and PJ34 + H/R group. The total protein was extracted from the cells in each group to detect the expressions of pADPr, Bax, the DNA damage marker protein p-YH2ax, and autophagic flow-associated proteins LC3BⅡ/LC3Ⅰ, Beclin-1, and P62 using Western blotting...
July 30, 2018: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
https://www.readbyqxmd.com/read/30168827/multi-parametric-cell-profiling-with-a-cmos-quad-modality-cellular-interfacing-array-for-label-free-fully-automated-drug-screening
#7
Jong Seok Park, Sandra I Grijalva, Moez K Aziz, Taiyun Chi, Sensen Li, Michael N Sayegh, Adam Wang, Hee Cheol Cho, Hua Wang
Cells are complex systems with concurrent multi-physical responses, and cell physiological signals are often encoded with spatiotemporal dynamics and further coupled with multiple cellular activities. However, most existing electronic sensors are only single-modality and cannot capture multi-parametric cellular responses. In this paper, a 1024-pixel CMOS quad-modality cellular interfacing array that enables multi-parametric cell profiling for drug development is presented. The quad-modality CMOS array features cellular impedance characterization, optical detection, extracellular potential recording, and biphasic current stimulation...
September 26, 2018: Lab on a Chip
https://www.readbyqxmd.com/read/30158990/cabbage-brassica-oleracea-var-capitata-protects-against-h-2-o-2-induced-oxidative-stress-by-preventing-mitochondrial-dysfunction-in-h9c2-cardiomyoblasts
#8
Dong Kwon Yang
Oxidative stress plays an important role in the progression of cardiac diseases, including ischemia/reperfusion injury, myocardial infarction, and heart failure. Growing evidence indicates that cabbage has various pharmacological properties against a wide range of diseases, such as cardiovascular diseases, hepatic diseases, and cancer. However, little is known about its effects on oxidative stress in cardiomyocytes or the underlying mechanisms. Therefore, the present study examined the effects of cabbage extract on oxidative stress in H9c2 cardiomyoblasts...
2018: Evidence-based Complementary and Alternative Medicine: ECAM
https://www.readbyqxmd.com/read/30140488/screening-potential-mitochondria-targeting-compounds-from-traditional-chinese-medicines-using-a-mitochondria-based-centrifugal-ultrafiltration-liquid-chromatography-mass-spectrometry-method
#9
Xing-Xin Yang, Yu-Zhen Zhou, Feng Xu, Jie Yu, Gegentana, Ming-Ying Shang, Xuan Wang, Shao-Qing Cai
Mitochondria regulate numerous crucial cell processes, including energy production, apoptotic cell death, oxidative stress, calcium homeostasis and lipid metabolism. Here, we applied an efficient mitochondria-based centrifugal ultrafiltration/liquid chromatography/mass spectrometry (LC/MS) method, also known as screening method for mitochondria-targeted bioactive constituents (SM-MBC). This method allowed searching natural mitochondria-targeting compounds from traditional Chinese medicines (TCMs), including Puerariae Radix (PR) and Chuanxiong Radix (CR)...
August 2018: Journal of Pharmaceutical Analysis
https://www.readbyqxmd.com/read/30135280/-influence-of-the-dense-extract-from-herb-of-primula-veris-l-on-the-oxidative-stress-development-and-the-functional-state-of-the-cardiomyocytes-mitochondria-of-rats-with-experimental-chronic-heart-failure
#10
T A Popova, E A Muzyko, M V Kustova, M A Bychenkova, V N Perfilova, I I Prokofiev, M A Samoylova, I N Tyurenkov, G M Latypova, V A Kataev
Experimental chronic heart failure (CHF), caused by administration of L-isoproterenol (2.5 mg/kg twice a day intraperitoneally for 21 days), promotes uncoupling of respiration and oxidative phosphorylation. The rate of mitochondrial oxygen consumption in the metabolic state V3 by Chance in animals with CHF decreased by 53.3% (p<0.05) with malate using (as an oxidation substrate feeding сomplex I of the electron transport chain (ETC)), by 70.6% (p<0.05) with succinate using (сomplex II substrate) and by 63...
August 2018: Biomedit︠s︡inskai︠a︡ Khimii︠a︡
https://www.readbyqxmd.com/read/30128816/anti-fibrotic-actions-of-roselle-extract-in-rat-model-of-myocardial-infarction
#11
Shafreena Shaukat Ali, Siti Fatimah Azaharah Mohamed, Nur Hafiqah Rozalei, Yap Wei Boon, Satirah Zainalabidin
Heart failure-associated morbidity and mortality is largely attributable to extensive and unregulated cardiac remodelling. Roselle (Hibiscus sabdariffa) calyces are enriched with natural polyphenols known for antioxidant and anti-hypertensive effects, yet its effects on early cardiac remodelling in post myocardial infarction (MI) setting are still unclear. Thus, the aim of this study was to investigate the actions of roselle extract on cardiac remodelling in rat model of MI. Male Wistar rats (200-300 g) were randomly allotted into three groups: Control, MI, and MI + Roselle...
August 20, 2018: Cardiovascular Toxicology
https://www.readbyqxmd.com/read/30121362/cardiac-procholecystokinin-expression-during-haemodynamic-changes-in-the-mammalian-heart
#12
Jens P Goetze, Ingrid Hunter, Nora E Zois, Dijana Terzic, Nana Valeur, L H Olsen, Julie Smith, Peter Plomgaard, Lasse H Hansen, Jens F Rehfeld, L Balling, Finn Gustafsson
Cardiac myocytes express the cholecystokinin gene (CCK) at propeptide level. We recently reported that cardiac CCK expression is acutely regulated by isoprenaline in a porcine model. The regulation of CCK expression after myocardial infarction, in exercise, and in severe heart failure is, however, unknown. Cardiac tissue was obtained from healthy new-born and adolescent farm pigs. Myocardial infarction was induced by coronary artery occlusion in adult minipigs. Healthy male subjects performed a 3-hour exercise test, and patients with severe heart failure referred for right heart catheterization were included...
August 16, 2018: Peptides
https://www.readbyqxmd.com/read/30077999/to-be-or-not-to-be-the-smoker-s-paradox-an-in-vitro-study
#13
Franziska Wittmann, Adrian Türkcan, Ulrike Baranyi, Eva Eichmair, Günther Laufer, David Bernhard, Barbara Messner
BACKGROUND/AIMS: Clinical studies have reported a better outcome of smokers after myocardial infarction compared to non-smokers. The data are controversial, as some clinical studies did not observe this effect. The cell biological processes involved, which might account for a 'Smoker's Paradox', have not been investigated yet. Therefore, the aim was to elucidate the effect of cigarette smoke on the viability of cardiomyocytes in the context of hypoxia and reperfusion. METHODS: HL-1 cells were incubated with different concentrations of cigarette smoke extract (CSE) and subjected to hypoxia/reperfusion to further evaluate influence of CSE on viability of HL-1 cells using flow cytometry analyses, Western Blot and immunofluorescence staining...
2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/30030530/astragaloside-iv-attenuates-myocardial-ischemia-reperfusion-injury-in-rats-via-inhibition-of-calcium-sensing-receptor-mediated-apoptotic-signaling-pathways
#14
Bo Yin, Xu-Wei Hou, Mei-Li Lu
Astragaloside IV (AsIV) is an active saponin extracted from Astragalus membranaceus, which has shown cardioprotective effects in a number of experimental animals. In this study we investigated the molecular mechanisms by which AsIV attenuated the myocardial ischemia reperfusion (MI/R)-induced injury in vitro and in vivo by focusing on calcium-sensing receptor (CaSR) and extracellular signal-regulated kinase 1/2 (ERK1/2). Rat neonatal cardiac myocytes were subjected to a hypoxia/reoxygenation (H/R) procedure in vitro, which significantly decreased the cell viability, increased lactate dehydrogenase (LDH) release, induced cardiomyocyte apoptosis, and increased [Ca2+ ]i ...
July 20, 2018: Acta Pharmacologica Sinica
https://www.readbyqxmd.com/read/30029882/amniotic-membrane-extracted-proteins-protect-h9c2-cardiomyoblasts-against-hypoxia-induced-apoptosis-by-modulating-oxidative-stress
#15
Yousef Faridvand, Samira Nozari, Simin Atashkhoei, Mohammad Nouri, Ahmadreza Jodati
Protection of the cardiac cell against hypoxia-induced cell damage is one of the main approaches to preventing cardiovascular disease. Earlier studies have shown the cardioprotective effect of the human Amniotic Membrane (hAM) in animal model of cardiac injury. However, the effect of Amniotic Membrane Proteins (AMPs), extracted from hAM, on myocardial hypoxia injury remains unclear. So, our study aimed to investigate the protective effect of AMPs against hypoxia-induced cardiomyocytes apoptosis. H9c2 cardiomyocytes were pre-treated with AMPs followed by 24 h in hypoxia condition...
September 10, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/30027630/automated-quantitative-analysis-of-multiple-cardiomyocytes-at-the-single-cell-level-with-three-dimensional-holographic-imaging-informatics
#16
Inkyu Moon, Keyvan Jaferzadeh, Ezat Ahmadzadeh, Bahram Javidi
Cardiomyocytes derived from human pluripotent stem cells are a promising tool for disease modelling, drug compound testing, and cardiac toxicity screening. Bio-image segmentation is a prerequisite step in cardiomyocyte image analysis by digital holography (DH) in microscopic configuration and has provided satisfactory results. In this study, we quantified multiple cardiac cells from segmented three-dimensional DH images at the single-cell level and measured multiple parameters describing the beating profile of each individual cell...
July 20, 2018: Journal of Biophotonics
https://www.readbyqxmd.com/read/30008784/effect-of-aqueous-extract-from-descurainia-sophia-l-webb-ex-prantl-on-ventricular-remodeling-in-chronic-heart-failure-rats
#17
Ying Luo, Zhiqiang Sun, Pengfei Hu, Yuanhong Wu, Weiwei Yu, Shuwei Huang
Objective: Descurainia sophia (L.) Webb ex Prantl (DS) is a traditional Chinese medicine. Our current study was to evaluate the effect of DS on ventricular remodeling in chronic heart failure (HF) rats and its underlying mechanism. Methods: The rat chronic heart failure model induced by suprarenal abdominal aortic coarctation surgery. The survival rats were randomly divided into 3 groups: the sham group (n=6), the HF group (n=6), and the HF+DS group (n=6). After 3 months of drug intervention, we examined the effects of DS by Sirius Red staining, electron microscopy, echocardiography, hemodynamic measurement, and TUNEL and explored the underlying mechanism by Western blotting...
2018: Evidence-based Complementary and Alternative Medicine: ECAM
https://www.readbyqxmd.com/read/29991602/solute-movement-in-the-t-tubule-system-of-rabbit-and-mouse-cardiomyocytes
#18
Cherrie H T Kong, Eva A Rog-Zielinska, Peter Kohl, Clive H Orchard, Mark B Cannell
Cardiac transverse (t-) tubules carry both electrical excitation and solutes toward the cell center but their ability to transport small molecules is unclear. While fluorescence recovery after photobleaching (FRAP) can provide an approach to measure local solute movement, extraction of diffusion coefficients is confounded by cell and illumination beam geometries. In this study, we use measured cellular geometry and detailed computer modeling to derive the apparent diffusion coefficient of a 1-kDa solute inside the t-tubular system of rabbit and mouse ventricular cardiomyocytes...
July 24, 2018: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29987809/isolation-of-atrial-and-ventricular-cardiomyocytes-for-in-vitro-studies
#19
Jelena Plačkić, Jens Kockskämper
High quality cardiomyocyte isolation is of critical importance for successful studies of myocardial function at the cellular and molecular level. Although previous work has established isolation procedures for various species, it still remains challenging to produce consistently a high yield of viable and healthy cardiomyocytes. The basis for the most successful and reproducible isolation of cardiomyocytes from intact hearts is the Langendorff retrograde perfusion technique. Here, we will illustrate in detail all practical aspects of the enzyme-based Langendorff isolation of rat atrial and ventricular cardiomyocytes...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29986596/dendropanax-morbifera-prevents-cardiomyocyte-hypertrophy-by-inhibiting-the-sp1-gata4-pathway
#20
Simei Sun, Tianyi Li, Li Jin, Zhe Hao Piao, Bin Liu, Yuhee Ryu, Sin Young Choi, Gwi Ran Kim, Ji Eun Jeong, An Jin Wi, Song Ju Lee, Hae Jin Kee, Myung Ho Jeong
An extract of Dendropanax morbifera branch exerts antioxidant, anti-inflammatory, antithrombotic, and anticancer activities. The purpose of this study was to investigate the effect of the extract in isoproterenol-induced cardiac hypertrophy. Phalloidin staining showed that treatment with the extract dramatically prevents isoproterenol-induced H9c2 cell enlargement and the expression of cardiac hypertrophic marker genes, including atrial natriuretic peptide (ANP) and B-type brain natriuretic peptide (BNP). Further, pretreatment with the extract decreased isoproterenol-induced GATA4 and Sp1 expression in H9c2 cells...
2018: American Journal of Chinese Medicine
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