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Cardiomyocytes and extract

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https://www.readbyqxmd.com/read/30510844/therapeutic-applications-of-adipose-derived-stem-cells-in-cardiovascular-disease
#1
REVIEW
Kyle Bruun, Erika Schermer, Anjali Sivendra, Emily Valaik, Reed B Wise, Rana Said, John R Bracht
Cardiovascular disease (CVD) is the number one cause of death globally, and new therapeutic techniques outside of traditional pharmaceutical and surgical interventions are currently being developed. At the forefront is stem cell-centered therapy, with adipose derived stem cells (ADSCs), an adult stem population, providing significant clinical promise. When introduced into damaged heart tissue, ADSCs promote cardiac regeneration by a variety of mechanisms including differentiation into new cardiomyocytes and secretion of paracrine factors acting on endogenous cardiac cells...
2018: American Journal of Stem Cells
https://www.readbyqxmd.com/read/30483130/screening-and-identification-of-cardioprotective-compounds-from-wenxin-keli-by-activity-index-approach-and-in-vivo-zebrafish-model
#2
Hao Liu, Xuechun Chen, Xiaoping Zhao, Buchang Zhao, Ke Qian, Yang Shi, Mirko Baruscotti, Yi Wang
Wenxin Keli (WXKL) is a widely used Chinese botanical drug for the treatment of arrhythmia, which is consisted of four herbs and amber. In the present study, we analyzed the chemical composition of WXKL using liquid chromatography coupled with high-resolution mass spectrometry (LC-HRMS) to tentatively identify 71 compounds. Through typical separate procession, the total extract of WXKL was divided into fractions for further bioassays. Cardiomyocytes and zebrafish larvae were applied for assessment. In vivo arrhythmia model in Cmlc2-GFP transgenic zebrafish was induced by terfenadine, which exhibited obvious reduction of heart rate and occurrence of atrioventricular block...
2018: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/30465799/cathepsin-k-deficiency-impairs-mouse-cardiac-function-after-myocardial-infarction
#3
Wenqian Fang, Aina He, Mei-Xiang Xiang, Yan Lin, Yajun Wang, Jie Li, Chongzhe Yang, Xian Zhang, Cong-Lin Liu, Galina K Sukhova, Natasha Barascuk, Lise Larsen, Morten Karsdal, Peter Libby, Guo-Ping Shi
BACKGROUND: Extracellular matrix metabolism and cardiac cell death participate centrally in myocardial infarction (MI). This study tested the roles of collagenolytic cathepsin K (CatK) in post-MI left ventricular remodeling. METHODS AND RESULTS: Patients with acute MI had higher plasma CatK levels (20.49 ± 7.07 pmol/L, n = 26) than those in subjects with stable angina pectoris (8.34 ± 1.66 pmol/L, n = 28, P = .01) or those without coronary heart disease (6...
November 19, 2018: Journal of Molecular and Cellular Cardiology
https://www.readbyqxmd.com/read/30452770/hcn4-gene-variations-in-sudden-unexplained-nocturnal-death-syndrome-in-the-southern-han-chinese-population
#4
Qiuping Wu, Qianhao Zhao, Kun Yin, Bing-Jie Hu, Jianding Cheng
Sudden unexplained nocturnal death syndrome (SUNDS) is widely considered to be related to hereditary fatal arrhythmias. Hyperpolarization-activated cyclic nucleotide-gated channel 4 (HCN4) channels are widely distributed in sinus myocytes and play a profound role in generating pacemaker electro-activity in cardiomyocytes. In the present study, the potential correlation between HCN4 gene variations and the occurrence of SUNDS was investigated. Genomic DNA was extracted from blood samples of both 119 unrelated SUNDS patients and 184 healthy individuals and screened for candidate HCN4 gene variants...
November 19, 2018: Journal of Forensic Sciences
https://www.readbyqxmd.com/read/30449737/effects-of-milrinone-on-inflammatory-response-related-gene-expressions-in-cultured-rat-cardiomyocytes
#5
Archana G Venakatesh, Johann J Mathew, Scott Coleman, Longqiu Yang, Geoffrey L Liu, Marilyn M Li, Henry Liu
Congestive heart failure (CHF) is defined as a cardiac dysfunction leading to low cardiac output and inadequate tissue perfusion. Intravenous positive inotropes are used to increase myocardial contractility in hospitalized patients with advanced heart failure. Milrinone is a phosphodiesterase Ⅲ inhibitor and used most commonly for inotropic effect. The well-known PROMISE study investigated the effects of milrinone on mortality in patients with severe CHF, and concluded that long-term therapy with milrinone increased morbidity and mortality among patients with advanced CHF...
November 18, 2018: Journal of Biomedical Research
https://www.readbyqxmd.com/read/30405071/study-on-cardiotoxicity-and-mechanism-of-fuzi-extracts-based-on-metabonomics
#6
Guangyao Huang, Liang Yang, Wei Zhou, Xianglin Tang, Yuguang Wang, Zengchun Ma, Shan Gao, Yue Gao
To investigate the toxicity of water and ethanol "Fuzi" (FZ) extracts and to explore the toxicity mechanism in rats. Water and ethanol extracts were prepared. Three groups of rats received the water extract, ethanol extract, or water by oral gavage for seven days. Pathological section staining of heart tissue. Colorimetric analysis was used to determine serum lactate dehydrogenase. The metabolic expression of small molecules in rats was measured by a metabolomics method. Western blotting was used to detect the expression of phosphoinositide 3-kinase (PI3K), protein kinase B (Akt), mammalian target of rapamycin (mTOR), transforming growth factor-β1 (TGF-β1), and caspase-3...
November 7, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/30399570/nrf2-activation-and-down-regulation-of-hmgb1-and-myd88-expression-by-amnion-membrane-extracts-in-response-to-the-hypoxia-induced-injury-in-cardiac-h9c2-cells
#7
Yousef Faridvand, Samira Nozari, Vahid Vahedian, Nasser Safaie, Masoud Pezeshkian, Parinaz Haddadi, Mina Mamipour, Arezoo Rezaie-Nezhad, Ahmadreza Jodati, Mohammad Nouri
BACKGROUND: human Amniotic Membrane (hAM) extracts contain bioactive molecules such as growth factors and cytokines. Studies have confirmed the ability of hAM in reduction of post-operative dysfunction in patients with cardiac surgery. However, the function of Amniotic Membrane Proteins (AMPs), extracted from hAM, against hypoxia-induced H9c2 cells injury have never been investigated. In this study, we aimed to appraise the protective impact of AMPs on H9c2 cells under hypoxia condition...
November 3, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/30374879/simultaneous-afm-investigation-of-the-single-cardiomyocyte-electro-chemo-mechanics-during-excitation-contraction-coupling
#8
Guido Caluori, Roberto Raiteri, Mariateresa Tedesco
The cardiac excitation-contraction coupling is the cellular process through which the heart absolves its blood pumping function, and it is directly affected when cardiac pathologies occur. Cardiomyocytes are the functional units in which this complex biomolecular process takes place: they can be represented as a two-stage electro-chemo and chemo-mechanical transducer, along which each stage can be probed and monitored via appropriate micro/nanotechnology-based tools. Atomic force microscopy (AFM), with its unique nanoresolved force sensitivity and versatile modes of extracting sample properties, can represent a key instrument to study time-dependent heart mechanics and topography at the single cell level...
2019: Methods in Molecular Biology
https://www.readbyqxmd.com/read/30362048/cardiac-mitochondrial-respiration-following-a-low-carbohydrate-high-fat-diet-in-apolipoprotein-e-deficient-mice
#9
Cynthia Rocha, Olivia H Koury, Celena Scheede-Bergdahl, Andreas Bergdahl
Low-carbohydrate diets are considered to be an effective approach to weight loss and have, subsequently, grown in popularity. Despite the apparent health benefits that these diets may provide for insulin resistance, hypertension, and dyslipidemia, their implications on cardiomyocyte oxidative capacity have yet to be investigated. To evaluate the adaptations induced by a 6-week low-carbohydrate, high-fat (LCHF) diet on mitochondrial respiration, two groups of male mice were investigated: Apolipoprotein E-deficient mice on a LCHF diet (L-DIET) and apolipoprotein E-deficient mice on a regular rodent diet (CON)...
October 25, 2018: Journal of Physiology and Biochemistry
https://www.readbyqxmd.com/read/30308230/meroterpenoids-from-ganoderma-sinense-protect-hepatocytes-and-cardiomyocytes-from-oxidative-stress-induced-injuries
#10
Suyu Gao, Peng Zhang, Cuiyun Zhang, Fengyan Bao, Hua Li, Lixia Chen
Four meroterpenoids, applanatumols F (1), H (3), I (2), and lingzhiol (4) were isolated from the 95% EtOH extract of the fruiting bodies of Ganoderma sinense. Their structures were established on the basis of NMR spectroscopic analyses, optical rotatory dispersion data, ECD spectra, and X-ray crystallography. Compounds 1, 2, 4 existed as racemic mixtures ((+) 1a, 2a, 4a; (-) 1b, 2b, 4b), while 3 as a single enantiomer. Base on the seperated enantiomers, we sought to explicit possible effects of compounds 1-4 on hydrogen peroxide (H2 O2 )-induced cell death and to determine their underlying molecular mechanisms in human normal liver LO2 cells...
October 9, 2018: Fitoterapia
https://www.readbyqxmd.com/read/30278018/label-free-identification-of-antibody-mediated-rejection-in-cardiac-allograft-biopsies-using-infrared-spectroscopic-imaging
#11
Imran Uraizee, Vishal K Varma, Hari Sreedhar, Francesca Gambacorta, Shaiju Nazeer, Aliya Husain, Michael J Walsh
BACKGROUND: Antibody-mediated rejection (AMR) in cardiac allograft recipients remains less well-understood than acute cellular rejection, is associated with worse outcomes, and portends a greater risk of developing chronic allograft vasculopathy. Diffuse immunohistochemical (IHC) C4d staining of capillary endothelia in formalin-fixed, paraffin-embedded (FFPE) right ventricular (RV) endomyocardial biopsies is diagnostic of immunopathologic AMR but serves more as a late-stage marker. Infrared (IR) spectroscopy may be a useful tool in earlier detection of rejection...
October 1, 2018: Transplantation
https://www.readbyqxmd.com/read/30256522/gentisic-acid-attenuates-pressure-overload-induced-cardiac-hypertrophy-and-fibrosis-in-mice-through-inhibition-of-the-erk1-2-pathway
#12
Simei Sun, Hae Jin Kee, Li Jin, Yuhee Ryu, Sin Young Choi, Gwi Ran Kim, Myung Ho Jeong
We previously reported that gentisic acid (2,5-dihydroxybenzoic acid) is the third most abundant phenolic component of Dendropanax morbifera branch extracts. Here, we investigated its effects on cardiac hypertrophy and fibrosis in a mouse model of pressure overload and compared them to those of the beta blocker bisoprolol and calcium channel blocker diltiazem. Cardiac hypertrophy was induced in mice by transverse aortic constriction (TAC). Beginning 2 weeks after this procedure, the mice were given daily intraperitoneal injections of gentisic acid (100 mg/kg/d), bisoprolol (5 mg/kg/d) or diltiazem (10 mg/kg/d) for 3 weeks...
December 2018: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/30241309/trichosanthis-pericarpium-aqueous-extract-protects-h9c2-cardiomyocytes-from-hypoxia-reoxygenation-injury-by-regulating-pi3k-akt-no-pathway
#13
Donghai Chu, Zhenqiu Zhang
Trichosanthis Pericarpium (TP) is a traditional Chinese medicine for treating cardiovascular diseases. In this study, we investigated the effects of TP aqueous extract (TPAE) on hypoxia/reoxygenation (H/R) induced injury in H9c2 cardiomyocytes and explored the underlying mechanisms. H9c2 cells were cultured under the hypoxia condition induced by sodium hydrosulfite for 30 min and reoxygenated for 4 h. Cell viability was measured by MTT assay. The amounts of LDH, NO, eNOS, and iNOS were tested by ELISA kits...
September 20, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/30199522/the-effects-of-dracocephalum-heterophyllum-benth-flavonoid-on-hypertrophic-cardiomyocytes-induced-by-angiotensin-ii-in-rats
#14
Hong Jiang, Chen Zhang, Wen He
BACKGROUND Dracocephalum heterophyllum Benth flavonoid (DHBF) is a Tibetan and Uighur traditional medicine used to treat various disorders such as hypertension, lung heat, cough, and bronchitis; it has good antioxidant activity. Previous studies have shown that DHBF can reduce blood pressure in renovascular hypertensive rats, improve left ventricular systolic and diastolic function, and improve myocardial contractility. Therefore, we aimed to study the effect of DHBF on cardiomyocyte hypertrophy in cultured cells...
September 10, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/30187876/-role-of-poly-adp-ribose-polymerases-1-mediated-blockade-of-autophagy-in-ischemia-reperfusion-injury-of-rat-cardiomyocytes
#15
Wei Zhao, Yongwei Wang, Guanshan Wei, Shiyuan Xu
OBJECTIVE: To investigate the role of poly(ADP-ribose) polymerases-1 (PARP-1)-mediated blockade of autophagic flow in myocardial ischemia-reperfusion injury. METHODS: H9c2 cells, a rat cardiac myocyte line, were divided into control group, hypoxia/ reoxygenation model group (H/R group), PARP-1 inhibitor (PJ34) group, and PJ34 + H/R group. The total protein was extracted from the cells in each group to detect the expressions of pADPr, Bax, the DNA damage marker protein p-YH2ax, and autophagic flow-associated proteins LC3BⅡ/LC3Ⅰ, Beclin-1, and P62 using Western blotting...
July 30, 2018: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
https://www.readbyqxmd.com/read/30168827/multi-parametric-cell-profiling-with-a-cmos-quad-modality-cellular-interfacing-array-for-label-free-fully-automated-drug-screening
#16
Jong Seok Park, Sandra I Grijalva, Moez K Aziz, Taiyun Chi, Sensen Li, Michael N Sayegh, Adam Wang, Hee Cheol Cho, Hua Wang
Cells are complex systems with concurrent multi-physical responses, and cell physiological signals are often encoded with spatiotemporal dynamics and further coupled with multiple cellular activities. However, most existing electronic sensors are only single-modality and cannot capture multi-parametric cellular responses. In this paper, a 1024-pixel CMOS quad-modality cellular interfacing array that enables multi-parametric cell profiling for drug development is presented. The quad-modality CMOS array features cellular impedance characterization, optical detection, extracellular potential recording, and biphasic current stimulation...
September 26, 2018: Lab on a Chip
https://www.readbyqxmd.com/read/30158990/cabbage-brassica-oleracea-var-capitata-protects-against-h-2-o-2-induced-oxidative-stress-by-preventing-mitochondrial-dysfunction-in-h9c2-cardiomyoblasts
#17
Dong Kwon Yang
Oxidative stress plays an important role in the progression of cardiac diseases, including ischemia/reperfusion injury, myocardial infarction, and heart failure. Growing evidence indicates that cabbage has various pharmacological properties against a wide range of diseases, such as cardiovascular diseases, hepatic diseases, and cancer. However, little is known about its effects on oxidative stress in cardiomyocytes or the underlying mechanisms. Therefore, the present study examined the effects of cabbage extract on oxidative stress in H9c2 cardiomyoblasts...
2018: Evidence-based Complementary and Alternative Medicine: ECAM
https://www.readbyqxmd.com/read/30140488/screening-potential-mitochondria-targeting-compounds-from-traditional-chinese-medicines-using-a-mitochondria-based-centrifugal-ultrafiltration-liquid-chromatography-mass-spectrometry-method
#18
Xing-Xin Yang, Yu-Zhen Zhou, Feng Xu, Jie Yu, Gegentana, Ming-Ying Shang, Xuan Wang, Shao-Qing Cai
Mitochondria regulate numerous crucial cell processes, including energy production, apoptotic cell death, oxidative stress, calcium homeostasis and lipid metabolism. Here, we applied an efficient mitochondria-based centrifugal ultrafiltration/liquid chromatography/mass spectrometry (LC/MS) method, also known as screening method for mitochondria-targeted bioactive constituents (SM-MBC). This method allowed searching natural mitochondria-targeting compounds from traditional Chinese medicines (TCMs), including Puerariae Radix (PR) and Chuanxiong Radix (CR)...
August 2018: Journal of Pharmaceutical Analysis
https://www.readbyqxmd.com/read/30135280/-influence-of-the-dense-extract-from-herb-of-primula-veris-l-on-the-oxidative-stress-development-and-the-functional-state-of-the-cardiomyocytes-mitochondria-of-rats-with-experimental-chronic-heart-failure
#19
T A Popova, E A Muzyko, M V Kustova, M A Bychenkova, V N Perfilova, I I Prokofiev, M A Samoylova, I N Tyurenkov, G M Latypova, V A Kataev
Experimental chronic heart failure (CHF), caused by administration of L-isoproterenol (2.5 mg/kg twice a day intraperitoneally for 21 days), promotes uncoupling of respiration and oxidative phosphorylation. The rate of mitochondrial oxygen consumption in the metabolic state V3 by Chance in animals with CHF decreased by 53.3% (p<0.05) with malate using (as an oxidation substrate feeding сomplex I of the electron transport chain (ETC)), by 70.6% (p<0.05) with succinate using (сomplex II substrate) and by 63...
August 2018: Biomedit︠s︡inskai︠a︡ Khimii︠a︡
https://www.readbyqxmd.com/read/30128816/anti-fibrotic-actions-of-roselle-extract-in-rat-model-of-myocardial-infarction
#20
Shafreena Shaukat Ali, Siti Fatimah Azaharah Mohamed, Nur Hafiqah Rozalei, Yap Wei Boon, Satirah Zainalabidin
Heart failure-associated morbidity and mortality is largely attributable to extensive and unregulated cardiac remodelling. Roselle (Hibiscus sabdariffa) calyces are enriched with natural polyphenols known for antioxidant and anti-hypertensive effects, yet its effects on early cardiac remodelling in post myocardial infarction (MI) setting are still unclear. Thus, the aim of this study was to investigate the actions of roselle extract on cardiac remodelling in rat model of MI. Male Wistar rats (200-300 g) were randomly allotted into three groups: Control, MI, and MI + Roselle...
August 20, 2018: Cardiovascular Toxicology
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