Fengqian Ma, Xuan Wang, Zhanghua Qiu, Shoupeng Ding, Wenya Du, Yumei Dai, Tao Ma, Linzhi Yue, Guofu Wang, Tao Wang, Ling Geng, Lixian Wu
Murine Natural Killer cells were cultivated in vitro to isolate NK-derived exosomes. Subsequent quantification via qPCR confirmed enrichment of miR-1249-3p. Ana-1 murine macrophages were cultured in vitro and subsequently inoculated with Mycobacterium tuberculosis (MTB) strain H37Rv. NK-exo and NK-exo miR-1249-3p were separately applied to the infection model, followed by immunological assays conducted post-48-hour co-culture. Western blot analyses corroborated that NK-exo exhibited exosomal marker proteins Granzyme A (GzmA), Granzyme B (GzmB), and Perforin (PFN), alongside a notable enrichment of miR-1249-3p...
December 29, 2023: Cytokine