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https://www.readbyqxmd.com/read/30328017/recombinant-neuraminidase-pseudotyped-baculovirus-a-dual-vector-for-delivery-of-angiotensin-ii-peptides-and-dna-vaccine
#1
Irisa Trianti, Saengchai Akeprathumchai, Phenjun Mekvichitsaeng, Sansanalak Rachdawong, Kanokwan Poomputsa
Baculovirus is a promising vaccine deliver vector due to its biosafety profiles, gene transfer efficiency, ability to display small foreign antigens on its surface, strong adjuvant activities, etc. A dual vector for peptide antigens and a DNA vaccine delivery was constructed. In this vector, a tetrameric glycoprotein neuraminidase (NA) from influenza A virus (H5N1) serves as a baculovirus surface protein to improve baculovirus transduction efficiency and a partner for displaying the target peptide antigen. Nucleotides encoding target peptides could be fused to a full length NA gene, at the lower part of its head structure, integrated into Autographa californica multinucleopolyhedrovirus genome and expressed under the control of a White Spot Syndrome Virus IE-1 shuttle promoter...
October 16, 2018: AMB Express
https://www.readbyqxmd.com/read/30324932/study-of-microbial-diversity-in-saliva-and-plaque-samples-from-caries-free-and-caries-affected-children-using-denaturing-gradient-gel-electrophoresis
#2
G A Raviraj, Kishore G Bhat, Manohar S Kugaji, Vijay M Kumbar, Amruta Hooli
Background: Recent investigations have shown the possible involvement of bacteria other than mutans group and Lactobacilli in the etiology of caries. Molecular methods have been used to study the microbial diversity in caries-active (CA) and caries-free (CF) children. Among them, denaturing gradient gel electrophoresis (DGGE) is more popular and has been used in the present study. Aims: The aim of the present study was to investigate the difference in bacterial diversity in saliva and plaque samples from CF and CA children using DGGE...
October 2018: Journal of the Indian Society of Pedodontics and Preventive Dentistry
https://www.readbyqxmd.com/read/30324799/long-term-follow-up-of-camouflage-effects-following-resin-infiltration-of-post-orthodontic-white-spot-lesions-in-vivo
#3
Michael Knösel, Amely Eckstein, Hans-Joachim Helms
OBJECTIVES: To reassess the long-term camouflage effects of resin infiltration (Icon, DMG, Hamburg, Germany) of white spot lesions (WSL) and sound adjacent enamel (SAE) achieved in a previous trial. The null hypothesis was tested that there were no significantly different CIE-L*a*b*-ΔE-values between WSL and SAE areas of assessment after at least 24 months (T24) compared to those at baseline (T0). MATERIALS AND METHODS: Of twenty subjects who received previous resin infiltration treatment of nteeth = 111 nonrestored, noncavitated postorthodontic WSL after multibracket treatment during a randomized controlled trial and were contacted 20 months after baseline, eight subjects (trial teeth nteeth = 40; m/f ratio 1/7; age range (mean; SD) 12-17 [15...
October 16, 2018: Angle Orthodontist
https://www.readbyqxmd.com/read/30324444/light-damage-models-of-retinal-degeneration
#4
Christian Grimm, Charlotte E Remé
The induction of retinal degeneration by light exposure is widely used to study mechanisms of cell death. The advantage of such light-induced lesions over genetically determined degenerations is that light exposures can be manipulated according to the needs of the experimenter. Bright white light exposure can induce a synchronized burst of apoptosis in photoreceptors in a large retinal area which permits to study cellular and molecular events in a controlled fashion. Blue light of high energy induces a hot spot of high retinal irradiance within very short exposure durations (seconds to minutes) and may help to unravel the initial events after light absorption which may be similar for all damage regimens...
2019: Methods in Molecular Biology
https://www.readbyqxmd.com/read/30320253/in-situ-immobilized-sesamol-quinone-carbon-nanoblack-based-electrochemical-redox-platform-for-efficient-bioelectrocatalytic-and-immunosensor-applications
#5
Mansi Gandhi, Desikan Rajagopal, Sampath Parthasarathy, Sudhakaran Raja, Sheng-Tung Huang, Annamalai Senthil Kumar
Most of the common redox mediators such as organic dyes and cyanide ligand-associated metal complex systems that have been used for various electrochemical applications are hazardous nature. Sesamol, a vital nutrient that exists in natural products like sesame seeds and oil, shows several therapeutic benefits including anticancer, antidiabetic, cardiovascular protective properties, etc. Herein, we introduce a new electrochemical redox platform based on a sesamol derivative, sesamol-quinone (Ses-Qn; oxidized sesamol), prepared by the in situ electrochemical oxidation method on a carbon nanoblack chemically modified glassy carbon electrode surface (GCE/CB@Ses-Qn) in pH 7 phosphate buffer solution, for nontoxic and sustainable electrochemical, electroanalytical, and bioelectroanalytical applications...
September 30, 2018: ACS Omega
https://www.readbyqxmd.com/read/30317398/swept-source-oct-in-patients-with-multiple-evanescent-white-dot-syndrome
#6
Felipe Pereira, Luiz H Lima, Alexandre Gomes B de Azevedo, Claudio Zett, Michel E Farah, Rubens Belfort
BACKGROUND: Swept-source optical coherence tomography (SS-OCT) has a higher scanning rate and longer wavelength in comparison with spectral-domain OCT (SD-OCT), allowing an improved imaging of retinal vascular plexuses and choriocapillaris. The present two patients diagnosed with multiple evanescent white dot syndrome (MEWDS) underwent fundus autofluorescence (FAF), en-face SS-OCT, and SS-OCT angiography (OCTA) imaging, and its features were described and correlated. RESULTS: The clinical and imaging findings of both cases were consistent with the diagnosis of MEWDS...
October 13, 2018: Journal of Ophthalmic Inflammation and Infection
https://www.readbyqxmd.com/read/30316944/functional-characterization-of-a-matrix-metalloproteinase-2-gene-in-litopenaeus-vannamei
#7
Yu-Ying Lian, Hong-Hui He, Chao-Zheng Zhang, Xin-Cang Li, Yi-Hong Chen
Matrix metalloproteinases (MMPs) contribute to both normal and pathological tissue remodeling. They act as regulatory molecules by working in enzyme cascades as well as processing matrix proteins, cytokines, growth factors and adhesion molecules to generate fragments with biological effects. So MMPs could play distrinct roles in the process of pathogen infection. In present study, we cloned a MMP-2 (LvMMP-2) gene from Litopenaeus vannamei. LvMMP-2, highly expressed in epidermis, located to endoplasmic reticulum in S2 cells...
October 11, 2018: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/30315423/effect-of-tif-4-varnish-on-microbiological-changes-and-caries-prevention-in-situ-and-in-vivo-models
#8
Adílis Kalina Alexandria, Camila Nassur, Carolina Bezerra Cavalcanti Nóbrega, Luciana Salles Branco-de-Almeida, Katia Regina Netto Dos Santos, Alexandre Rezende Vieira, Aline Almeida Neves, Pedro Luiz Rosalen, Ana Maria Gondim Valença, Lucianne Cople Maia
OBJECTIVE: The aim of this study was to evaluate microbiological changes, oral soft tissue toxicity, and caries-preventive effect of an experimental titanium tetrafluoride (TiF4 ) varnish compared with a commercially available fluoride varnish (NaF), using in situ and in vivo models. MATERIALS AND METHODS: The treatment groups were the following: TiF4 varnish (experimental varnish), Duraphat® (fluoride positive control), placebo varnish (no fluoride), and no treatment (negative control)...
October 12, 2018: Clinical Oral Investigations
https://www.readbyqxmd.com/read/30314232/the-freshwater-snake-eel-genus-lamnostoma-anguilliformes-ophichthidae-in-taiwan-with-description-of-a-new-species
#9
Yung-Chieh Chiu, Kwang-Tsao Shao, Shih-Pin Huang, Hong-Ming Chen
A review of the freshwater ophichthid eel genus Lamnostoma from Taiwan is provided with three species recognized. Lamnostoma mindora has been recorded in previous literature and its presence is confirmed. Two specimens of Lamnostoma polyophthalmum represent the first record in Taiwan. A new species, Lamnostoma taiwanense sp. nov., is described on the basis of two specimens collected from northeastern Taiwan. It can be distinguished by having 3 preopercular pores, higher number of teeth on lower jaw, and a series of white spots with hill-shaped papillae along the head...
July 31, 2018: Zootaxa
https://www.readbyqxmd.com/read/30314207/a-new-species-of-the-genus-xya-latreille-1809-from-shandong-china-orthoptra-tridctyloidea-tridactylidae
#10
Da-Peng Zhang, Hai-Xiang Yin, Zhan Yin
The genus Xya Latreille, 1808 obtains 56 known species in the world, among them 20 species distributed in Asia and 7 species in China only i.e. Xya japonica (Haan, 1842); Xya nitobei (Shiraki, 1911); Xya manchurei Shiraki, 1936; Xya apicicornis (Chopard, 1928); Xya riparia (Saussure, 1877); Xya leshanensis Cao et al, 2017 and Xya sichuanensis Cao et al, 2018, [Haan, 1844; Walker, 1871; Saussure, 1877, 1896; Brunner von Wattenwyl. 1893; Bolivar, 1900(1899); Shiraki, 1911, 1936; Chopard, 1928, 1936, 1968; Bey-Bienko, 1967; Günther, 1974...
August 3, 2018: Zootaxa
https://www.readbyqxmd.com/read/30314181/a-new-species-of-short-fingered-geckos-stenodactylus-squamata-geckonidae-from-south-iran-with-taxonomic-notes-on-validity-of-the-genus-trigonodactylus-hass-1957
#11
Roman A Nazarov, Daniel A Melnikov, Mehdi Radjabizadeh, Nikolay A Poyarkov
In the present study we provide evidence for the validity of the genus Trigonodactylus Hass, 1957, improve the diagnosis for this genus and describe a new species that belongs to it-Trigonodactylus persicus sp. nov., from the sand dunes in Khuzestan Province, southwestern Iran. The new species is closely related to Trigonodactylus [Stenodactylus] arabicus sensu Hass, and can be distinguished by the following morphological characteristics: small size, maximum SVL 34 mm; SVL/TailL-approximately 1:1; ventral scales roundish, weakly keeled, 54-61 longitudinal rows at midbody and 190-25 along midbody...
August 7, 2018: Zootaxa
https://www.readbyqxmd.com/read/30314095/new-species-of-heteropterna-skuse-and-keroplatus-bosc-diptera-keroplatidae-become-the-second-records-of-this-family-for-cuba
#12
Jorge Mederos
Two new species of Keroplatini (Diptera: Keroplatidae: Keroplatinae), Heteropterna (Heteropterna) garae n. sp. and Keroplatus maxi n. sp. are described from Cuba, the second record of the family from this territory since Matile's paper in 1977. Heteropterna garae n. sp. is characterized by the last three flagellomeres being a silvery white colour (the last with a light brown apical border), the wings with a distinct colour pattern, and the unique shape of the terminalia and gonostylus. Keroplatus maxi n. sp...
August 20, 2018: Zootaxa
https://www.readbyqxmd.com/read/30313747/description-of-four-new-species-of-freshwater-gobies-from-the-black-river-drainage-in-china-and-vietnam-teleostei-gobiidae
#13
Marco Endruweit
Four new species of Rhinogobius Gill 1859 are described from the Black River drainage in China and Vietnam. Rhinogobius coccinella, new species, and R. nanophyllum, new species, are described from the Mengyejiang River in Yunnan, China. Rhinogobius coccinella is diagnosed by having the cheek and operculum with about 25 large dark spots and branchiostegal membrane unmarked; caudal-peduncle depth 11-12% SL; 1-5 predorsal scales; 27-30 midlateral scales; and 28 vertebrae. Rhinogobius nanophyllum is diagnosed by having the cheek, operculum and pectoral-fin base with about 90 indistinct white marks, caudal fin plain-colored, second dorsal fin magnified with a very broad dark gray median band and a broad white proximal band in males; caudal-peduncle length 22-25% SL and pectoral-fin length 21-24% SL in males; head width at operculum 53-58% HL in males, 58-61% HL in females; 0 predorsal scales; 8-10 branched rays in the second dorsal fin; 7-8 branched anal-fin rays; and 28 vertebrae...
September 28, 2018: Zootaxa
https://www.readbyqxmd.com/read/30313613/a-new-species-of-cascade-frog-amphibia-ranidae-in-the-amolops-monticola-group-from-china
#14
Zhiyong Yuan, Jieqiong Jin, Jiannan Li, Bryan L Stuart, Jun Wu
A new species, Amolops wenshanensis sp. nov., is described from Guangxi and Yunnan Provinces, China. The new species is a member of the A. monticola group, and is distinguished from its congeners using morphological and molecular data. The new species is diagnosed by having glandular dorsolateral folds; smooth skin; side of head dark with a light-colored upper lip stripe extending to axilla; green dorsal coloration in life; immaculate venter; indistinct transverse bands on dorsal surfaces of limbs; tympanum distinct; pineal body absent; all fingertips expanded with circummarginal grooves; two oblique vomerine teeth; vocal sac and white nuptial pad present in males; supratympanic fold absent; outer metatarsal tubercle absent; glandular gold-white flank spot absent; and skin on venter not translucent...
May 1, 2018: Zootaxa
https://www.readbyqxmd.com/read/30313587/a-new-garden-eel-heteroconger-fugax-congridae-heterocongrinae-from-the-northwestern-pacific-ocean
#15
Keita Koeda, Takuma Fujii, Hiroyuki Motomura
Heteroconger fugax sp. nov. (Congridae: Heterocongrinae) is described from a single specimen collected from Amami-oshima island, Japan. The new species is most similar to Heteroconger tomberua Castle Randall 1999, known from Fiji and New Caledonia, in having a remarkably slender body with numerous small spots and a vertebral count close to 200. However, it can be distinguished from H. tomberua by the presence of a large distinct white blotch on the opercle; more numerous, dense spots over the entire head, including lips; ground color of body uniformly cream, without microscopic melanophores; numerous small conical cirri on the chin; and dorsal-fin origin located more posteriorly to appressed pectoral-fin tip...
May 8, 2018: Zootaxa
https://www.readbyqxmd.com/read/30313420/schistura-alboguttata-a-new-loach-species-of-the-family-nemacheilidae-pisces-cypriniformes-from-the-pearl-river-basin-in-guangxi-south-china
#16
Liang Cao, E Zhang
Schistura alboguttata, a new species of nemacheilid loach, is herein described from the Leli-He, a tributary flowing to the You-Jiang of the Pearl River basin (Zhu-Jiang in Chinese) at Tianlin County, Guangxi, South China. This new species can be readily distinguished from all other Chinese species of Schistura by its striking body coloration consisting of irregular white spots scattered over the dorsal and lateral regions of the body, with occasional irregular bars with narrow interspaces on the predorsal region...
September 4, 2018: Zootaxa
https://www.readbyqxmd.com/read/30313346/rhinogobius-maculagenys-a-new-species-of-freshwater-goby-teleostei-gobiidae-from-hunan-china
#17
Qianqian Wu, Xuejian Deng, Yanjie Wang, Yong Liu
A new freshwater goby, Rhinogobius maculagenys sp. nov., was collected from Hunan Province in Southern China. This species can be distinguished from all congeners by a combination of the following features: first dorsal fin with 6 spines; second dorsal fin with a single spine and 7-9 segmented rays; anal fin with a single spine and 6-8 segmented rays; pectoral fin with 16 segmented rays; 32-34 longitudinal scales; 9-13 transverse scales; 11+16=27 vertebrae; pore ω1 missing; head and body yellowish brown; cheek and opercle yellowish brown with over 30 small orange spots, branchiostegal membrane yellow with over 10 small orange spots in males and white and spotless in females; first dorsal fin trapezoidal in males and nearly semicircular in females, with large bright blue blotch in front of second spine; spines 4 and 5 longest, rear tip extending to base of second branched ray of second dorsal fin in males when adpressed, but just reaching or not reaching anterior margin of second dorsal fin in females; caudal fin with 5-6 vertical rows of brown spots; flank with several longitudinal rows of blackish-brown spots; and belly pale white...
September 13, 2018: Zootaxa
https://www.readbyqxmd.com/read/30311669/pathogenicity-of-pasteurella-sp-in-lumpsuckers-cyclopterus-lumpus-l
#18
Rebecca Marie Ellul, Cecilie Walde, Gyri Teien Haugland, Heidrun Wergeland, Anita Rønneseth
The incidence of disease caused by Pasteurella sp. in farmed lumpsuckers in Norway has been steadily increasing in recent years, causing significant economic losses and fish welfare issues. The disease affects all life stages, both in hatcheries and after release into salmon cages. Therefore, it is important to establish robust challenge models, to be used for vaccine development. Exposure experiments via intramuscular and intraperitoneal injection underlined the high virulence of the bacteria, whereas the cohabitation and bath models allowed the chronic symptoms of the disease to be studied more accurately...
October 12, 2018: Journal of Fish Diseases
https://www.readbyqxmd.com/read/30308308/design-and-implementation-of-novel-hyperspectral-imaging-for-dental-carious-early-detection-using-laser-induced-fluorescence
#19
Yasser H El-Sharkawy, Sherif Elbasuney
Early detection of carious is vital for demineralization reverse offering less pain, as well as precise carious removal. In this study, the difference in optical properties of normal tissue and human carious lesion has been used for early diagnosis, using laser induced fluorescence spectroscopy. The optical system consists of light source in visible band and hyperspectral camera, associated with designed digital image processing algorithm. The human tooth sample was illuminated with visible band sources at 488, and 514 nm with energy of 5 m watt...
October 8, 2018: Photodiagnosis and Photodynamic Therapy
https://www.readbyqxmd.com/read/30303562/on-the-accuracy-and-precision-of-planet-for-multiparametric-mri-using-phase-cycled-bssfp-imaging
#20
Yulia Shcherbakova, Cornelis A T van den Berg, Chrit T W Moonen, Lambertus W Bartels
PURPOSE: In this work we demonstrate how sequence parameter settings influence the accuracy and precision in T1 , T2 , and off-resonance maps obtained with the PLANET method for a single-component signal model. In addition, the performance of the method for the particular case of a two-component relaxation model for white matter tissue was assessed. METHODS: Numerical simulations were performed to investigate the influence of sequence parameter settings on the accuracy and precision in the estimated parameters for a single-component model, as well as for a two-component white matter model...
October 10, 2018: Magnetic Resonance in Medicine: Official Journal of the Society of Magnetic Resonance in Medicine
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