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L-arginine and preeclampsia

Seidu Adams, Dongsheng Che, Guixin Qin, Mohammed Hamdy Farouk, Jiang Hailong, Han Rui
L-Homoarginine (hArg) ((2S)-amino-6-Carbamimidamidohexanoic acid) is a non-essential cationic amino acid that may be synthesised from the lysine catabolism or the transamination of its precursor (Arginine: Arg). These processes involve the use of the ornithine transcarbamoylase (OTC), an enzyme from the urea cycle or the arginine: glycine amidinotransferase (AGAT), an enzyme from the creatine biosynthesis pathway. These enzymes are tissue-specific, hence they synthesised L-hArg in animals and human organs such as the liver, kidneys, brains, and the small intestines...
October 26, 2018: Current Protein & Peptide Science
Regina A Nascimento, José S Possomato-Vieira, Victor H Gonçalves-Rizzi, Gisele F Bonacio, Elen Rizzi, Carlos A Dias-Junior
Preeclampsia is manifested as maternal hypertension and fetal growth restriction. Matrix metalloproteinases (MMPs) are involved in hypertension and doxycycline reduces blood pressure by inhibition of MMPs. Moreover, excessive levels of MMPs and reduced nitric oxide (NO) bioavailability have been related to preeclampsia. We investigated the involvement of MMPs in hypertension in pregnancy induced by Nω-Nitro-L-arginine methyl ester (L-NAME) in rats. To this end, zimography was performed to evaluate the activity of MMPs -2 and -9 in placenta, uterus and thoracic aorta, and systolic blood pressure, feto-placental development and metabolites of NO were evaluated...
December 5, 2018: European Journal of Pharmacology
Jeremy A Sandgren, Guorui Deng, Danny W Linggonegoro, Sabrina M Scroggins, Katherine J Perschbacher, Anand R Nair, Taryn E Nishimura, Shao Yang Zhang, Larry N Agbor, Jing Wu, Henry L Keen, Meghan C Naber, Nicole A Pearson, Kathy A Zimmerman, Robert M Weiss, Noelle C Bowdler, Yuriy M Usachev, Donna A Santillan, Matthew J Potthoff, Gary L Pierce, Katherine N Gibson-Corley, Curt D Sigmund, Mark K Santillan, Justin L Grobe
Copeptin, a marker of arginine vasopressin (AVP) secretion, is elevated throughout human pregnancies complicated by preeclampsia (PE), and AVP infusion throughout gestation is sufficient to induce the major phenotypes of PE in mice. Thus, we hypothesized a role for AVP in the pathogenesis of PE. AVP infusion into pregnant C57BL/6J mice resulted in hypertension, renal glomerular endotheliosis, intrauterine growth restriction, decreased placental growth factor (PGF), altered placental morphology, placental oxidative stress, and placental gene expression consistent with human PE...
October 4, 2018: JCI Insight
Jing Feng, Xinling Wang, Hongyan Li, Li Wang, Zengjun Tang
Preeclampsia (PE) is a disorder that is characterized by pregnancy‑induced hypertension. It has been reported that Annexin A1 (ANXA1) is highly expressed in the plasma of women diagnosed with PE. Therefore, the present study aimed to examine the effect of ANXA1 on PE rats. The PE animal model was constructed in rats using Nω‑nitro‑L‑arginine methyl ester (L‑NAME), and the blood pressure and urine protein levels of rats were detected. The pathological features of placental tissue, and the levels of inflammatory factors and ANXA1 were respectively measured by hematoxylin‑eosin staining, enzyme‑linked immunosorbent assay and immunohistochemical assay...
September 18, 2018: International Journal of Molecular Medicine
Mutlu Kucuk, Canan Ugur Yilmaz, Nurcan Orhan, Bulent Ahishali, Nadir Arican, Imdat Elmas, Candan Gürses, Mehmet Kaya
BACKGROUND: Preeclampsia is a disorder characterized by high blood pressure and often proteinuria during pregnancy. It is known that a subseptic dose of bacterial lipopolysaccharide (LPS) induces production of proinflammatory cytokines, and possibly increasing the risk for developing preeclampsia. We investigated the effects of LPS on the blood-brain barrier (BBB) integrity in pregnant rats with N(omega)-nitro-l-arginine methyl ester (L-NAME) induced preeclampsia. METHODS: Starting from the 10th day of gestation, pregnant rats were given L-NAME for 10 days to produce hypertension and proteinuria...
September 11, 2018: Journal of Stroke and Cerebrovascular Diseases: the Official Journal of National Stroke Association
Yuanyuan Chen, Fengxia Xue, Cha Han, Huiyun Yang, Lulu Han, Ke Li, Jie Li, Qian Xu, Zengyan Li, Bibo Yuan, Limin Yu, Xiaoli Gao, Ye Yan
Preeclampsia (PE) occurs specifically during pregnancy characterized by new-onset hypertension. The pathogenesis of PE was complicated, and inflammation may be central to the pathogenesis of PE. Ferulic acid (FA) is recognized to prevent cell damage and apoptosis induced by oxidative stress and inflammation. In our study, we used NG-nitro-l-arginine methyl ester (L-NAME)-induced rat model of PE to investigate whether FA improved PE and its possible mechanism. We found that FA significantly reduced blood pressure, urine volume, and urinary protein level in rats with PE...
September 5, 2018: Clinical and Experimental Hypertension: CHE
Shenghong Zhang, Cuili Zou, Qiaoqin Zhang
Preeclampsia, a serious multisystem disorder specific to human pregnancy, remains a considerable burden of disease worldwide. Reduced nitric oxide bioavailability is proved to be crucial in the maternal and fetal pathophysiology of preeclampsia. G-protein-coupled Receptor Kinase Interactor-1 (GIT1) is a novel endothelial nitric oxide synthases (eNOS) interactor mediator. The aim of this paper is to investigate the effect of GIT1 on preeclampsia. Blood pressure (BP) was measured using a carotid catheter-calibrated eight-chamber tail-cuff system (CODA) at the same time daily...
October 3, 2018: G3: Genes—Genomes—Genetics
G O Oludare, H D Jinadu, O O Aro
This study investigated the effect of L-arginine on blood pressure, angiogenic factors and liver enzymes in pregnant rats administered L-NAME. Thirty-six female Sprague-Dawley rats weighing between 150-170 g were divided into 4 groups of the control (normal saline), L-NAME (50 mg/kg b.w. intraperitoneal injection from days 13-18 of pregnancy), L-NAME + L-arginine (50 mg/kg b.w. and 1 g/kg b.w of L-arginine from days 13 to 18) and L-arginine (1 g/kg b.w administered orally from days 13 to 18). Urine samples were collected on day 18 of pregnancy and the animals were sacrificed on day 19 of pregnancy for blood pressure parameters, angiogenic and liver enzyme assays...
August 7, 2018: Pathophysiology: the Official Journal of the International Society for Pathophysiology
Wen Shu, Hanying Li, Hao Gong, Mei Zhang, Xiulong Niu, Yongqiang Ma, Xin Zhang, Wei Cai, Guohong Yang, Maoti Wei, Ning Yang, Yuming Li
Preeclampsia is a pregnancy-specific disease characterized by hypertension as well as proteinuria after the 20th week of pregnancy. Animal models are effective tools for studying the pathogenesis, diagnostic criteria and treatment methods of preeclampsia. The present study sought to establish and evaluate a preeclampsia-like Sprague Dawley (SD) rat model using N-nitro-L-arginine methyl ester (L-NAME). Rats were randomly assigned to 7 groups (n=10 in each): Control rats and rats treated with low-dose L-NAME (40 mg/kg body weight/day) starting from gestational day (GD) 9, medium-L-NAME (75 mg/kg body weight/day) starting from GD 9 (9D ML group), high-dose L-NAME (125 mg/kg body weight/day) starting from GD 9, low-dose L-NAME starting from GD 10, medium-dose L-NAME starting from GD 10 and high-dose L-NAME starting from GD 10...
August 2018: Experimental and Therapeutic Medicine
M B Tenório, R C Ferreira, F A Moura, N B Bueno, M O F Goulart, A C M Oliveira
AIMS: To determine whether oral antioxidant therapies, of various types and doses, are able to prevent or treat women with preeclampsia. DATA SYNTHESIS: The following databases were searched: MEDLINE, CENTRAL, LILACS, and Web of Science. Inclusion criteria were: a) randomized clinical trials; b) oral antioxidant supplementation; c) study in pregnant women; d) control group, treated or not with placebo. Papers were excluded if they evaluated antioxidant nutrient supplementation associated with other non-antioxidant therapies...
September 2018: Nutrition, Metabolism, and Cardiovascular Diseases: NMCD
Zhi-Hui Xiong, Jing Wei, Meng-Qi Lu, Mei-Yuan Jin, Hong-Ling Geng
AIM: This study aims to investigate the effect of human umbilical cord mesenchymal stem cell exosomes (hucMSC-Ex) on placental tissue and angiogenesis in rats with preeclampsia (PE). METHOD: The expression of MSC surface markers were identified by flow cytometry. Alizarin red staining and oil red O staining were used to examine osteogenic and adipogenic differentiation of hucMSCs. Western blotting was used to determine expressions of CD63 and CD81 in hucMSC-Ex. PE rat models were established using endothelial nitric oxide synthase, eNOS)NG -Nitro-l-arginine Methyl Ester, which were then treated with exosome (Exo) of low dosage (L-Exo), Exo of medium dosage (M-Exo) and Exo of high dosage (H-Exo)...
September 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
B Fatemeh Nobakht M Gh
Preeclampsia is a multifactorial disorder defined by hypertension and increased urinary protein excretion during pregnancy. It is a significant cause of maternal and neonatal deaths worldwide. Despite various research efforts to clarify pathogenies of preeclampsia and predict this disease before beginning of symptoms, the pathogenesis of preeclampsia is unclear. Early prediction and diagnosis of women at risk of preeclampsia has not markedly improved. Therefore, the objective of this study was to perform a review on metabolomic articles assessing predictive and diagnostic biomarkers of preeclampsia...
July 2, 2018: Systems Biology in Reproductive Medicine
Amani N Shafik, Mahmoud A Khattab, Ahmed H Osman
OBJECTIVE: Preeclampsia represents a major complication of pregnancy, associated with greater maternal and fetal complications. We compared the effects of esomeprazole (a proton pump inhibitor) and magnesium sulfate (MgSO4) on the deleterious effects observed on the mother and neonates in experimentally induced preeclampsia in rats. STUDY DESIGN: Preeclampsia was induced in pregnant rats with NG-nitro-l-arginine methyl ester (L-NAME) starting from day 10-till end of pregnancy...
June 2018: European Journal of Obstetrics, Gynecology, and Reproductive Biology
Sha Xiao, Ming Zhang, Zhenzhen He, Deling Wang
Preeclampsia is reported in pregnant women around the world and often causes maternal/fetal mortality and morbidity. In the current study, we assessed the efficacy of celastrol on a rat preeclampsia model induced by Nω -nitro-L-arginine methyl ester hydrochloride (L-NAME). Pregnant rats were administered L-NAME to establish preeclampsia. A total of 48 animals were randomly assigned into 4 groups (n = 12 each): control, control plus celastrol treatment (control+celastrol), preeclampsia, and preeclampsia plus celastrol...
July 5, 2018: European Journal of Pharmacology
Marie Lémery Magnin, Victor Fitoussi, Nathalie Siauve, Laetitia Pidial, Daniel Balvay, Gwennhael Autret, Charles André Cuenod, Olivier Clément, Laurent Julien Salomon
PURPOSE: To evaluate placental function and perfusion in a rat model of preeclampsia infused with L-nitro-arginine methyl ester (L-NAME) by dynamic contrast-enhanced (DCE) MRI using gadolinium chelates. METHODS: Pregnant female Sprague-Dawley rats were fitted on embryonic day 16 (E16) with subcutaneous osmotic minipumps loaded to deliver, continuously, L-NAME (50 mg/day per rat; case group) or saline solution (control group). DCE MRI was performed on E19 using gadolinium chelates and a 4...
2018: Fetal Diagnosis and Therapy
Cheng-Juan Sun, Ya Jin, Wei-Yuan Zhang, Lin Li, Xiao-Wei Liu
Proteinuria is a common adverse effect of renal injury in preeclampsia. To explore the effects of AKR1C3 in renal injury due to preeclampsia and to determine an optimal method to prevent proteinuria, glibenclamide treatment was used in unrestrained Wistar rats exposed to N-nitro-L-arginine methyl ester hydrochloride (L-NAME). Successful rat models for preeclampsia were confirmed based on mean arterial pressure, a 24-h protein urine test, and by observing the structure of the kidney by transmission electron microscopy (TEM)...
July 1, 2018: Gene
Jing Huai, Zi Yang, Yan-Hong Yi, Guang-Jiao Wang
Background: Pravastatin (Pra) exerts protective effects on preeclampsia. Preeclampsia is a multifactorial and pathogenic pathway syndrome. The present study compared the effects of Pra on clinical manifestations of preeclampsia in different pathogenic pathways. Methods: Two different preeclampsia-like mouse models used in this study were generated with Nω-nitro-L-arginine methyl ester (L-NAME) and used lipopolysaccharide (LPS) from day 7 of gestation, respectively...
February 20, 2018: Chinese Medical Journal
Sabrina M Scroggins, Donna A Santillan, Jenna M Lund, Jeremy A Sandgren, Lindsay K Krotz, Wendy S Hamilton, Eric J Devor, Heather A Davis, Gary L Pierce, Katherine N Gibson-Corley, Curt D Sigmund, Justin L Grobe, Mark K Santillan
The pathogenesis of preeclampsia (PreE), a hypertensive disorder of pregnancy, involves imbalanced T helper (TH ) cell populations and resultant changes in pro- and anti-inflammatory cytokine release. Elevated copeptin (an inert biomarker of arginine vasopressin (AVP)), secretion precedes the development of symptoms in PreE in humans, and infusion of AVP proximal to and throughout gestation is sufficient to initiate cardiovascular and renal phenotypes of PreE in wild-type C57BL/6J mice. We hypothesize that AVP infusion in wild-type mice is sufficient to induce the immune changes observed in human PreE...
February 14, 2018: Clinical Science (1979-)
Zhan Zhang, Xiaofang Wang, Jinming Wang, Linlin Zhang
This study aims to investigate the expression of Stat3 and p-Stat3 in the placenta of a preeclampsia-like rat model induced by Nωnitro-L-arginine methyl ester (L-NAME). Two-to three-month-old (20 males, 40 females) Sprague-Dawley rats were used in this study. After conception was confirmed by vaginal smears, on the thirteenth day of pregnancy, the animals were allocated into two groups: control (0.9% NaCl administered) group and L-NAME (75 mg/kg) group. After the treatment of L-NAME, there was a significant increase in systolic blood pressure (SBP) levels in the L-NAME group (148...
April 2018: Journal of Molecular Histology
Hayam Ibrahim Gad Allam, Abeer AbdulMoati Al Masri
OBJECTIVE: To investigate the role of peroxisome proliferator-activated receptor (PPAR)-γ agonist on renal functions and blood levels of vasoregulatory peptides by administering rosiglitazone-a PPAR-γ agonist- to nitric oxide (NO) synthase lacking pregnant rats as a creature model of preeclampsia. STUDY DESIGN: Experimental study. PLACE AND DURATION OF STUDY: Medical College at King Khaled University Hospital, Riyadh, Saudi Arabia, from July 2016 to May 2017...
January 2018: Journal of the College of Physicians and Surgeons—Pakistan: JCPSP
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