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L arginin

Consuelo Pasten, Cristóbal Alvarado, Jocelyn Rocco, Luis Contreras, Paula Aracena, Jéssica Liberona, Cristian Suazo, Luis Fernando Michea, Carlos E Irarrázabal
Upon renal ischemia-reperfusion (IR) injury, recruitment of neutrophils during the inflammatory process promotes local generation of oxygen and nitrogen reactive species which, in turn, are likely to exacerbate tissue damage. The mechanism by which iNOS is involved in IR has not been completely elucidated. In this work, the selective iNOS inhibitor L-N6 -(1-iminoethyl)lysine (L-NIL) and the NOS substrate L-Arginine (L-Arg), were employed to understand the role of NOS activity on the expression of particular target genes and the oxidative stress elicited after a 30 min of bilateral renal ischemia, followed by 48h reperfusion in BALB/c mice...
December 5, 2018: American Journal of Physiology. Renal Physiology
Merhan Mamdouh Ragy, Sabreen Mahmoud Ahmed
Diabetes and cardiometabolic risk factors including hypertension and dyslipidemia are the major threats to human health in the 21st century. Apoptosis in pancreatic tissue is one of the major causes of diabetes type 1 progression. The aim of this study was to investigate the effects of C-peptide or l-arginine on some cardiometabolic risk factors, pancreatic morphology, function and apoptosis, and the mechanisms of their actions. Forty adult male albino rats were divided into four equal groups: 1-control nondiabetic, 2-diabetic (no treatment), 3-diabetic + C-peptide, and 4-diabetic +  l-arginine...
December 4, 2018: Journal of Cellular Physiology
Kamil Kus, Agnieszka Kij, Agnieszka Zakrzewska, Agnieszka Jasztal, Marta Stojak, Maria Walczak, Stefan Chlopicki
BACKGROUND: The early detection of metastasis based on biomarkers in plasma may improve cancer prognosis and guide treatment. The aim of this work was to characterize alterations in metabolites of the arginine pathway, energy metabolism, and structural and signalling lipids in plasma in the early and late stages of murine breast cancer metastasis. METHODS: Mice were orthotopically inoculated with 4T1 metastatic breast cancer cells, and plasma was analysed along the pulmonary metastasis progression using LC-MS/MS-based targeted metabolomics and lipidomics...
December 4, 2018: Breast Cancer Research: BCR
Wen-Tsan Weng, Chieh-Shan Wu, Feng-Sheng Wang, Chang-Yi Wu, Yi-Ling Ma, Hoi-Hung Chan, Den-Chiung Wu, Jian-Ching Wu, Tian-Huei Chu, Shih-Chung Huang, Ming-Hong Tai
α-melanocyte-stimulating hormone (α-MSH) has been characterized as a novel angiogenesis inhibitor. The homeostasis of nitric oxide (NO) plays an important role in neovascularization. However, it remains unclear whether α-MSH mitigates angiogenesis through modulation of NO and its signaling pathway. The present study elucidated the function and mechanism of NO signaling in α-MSH-induced angiogenesis inhibition using cultured human umbilical vein endothelial cells (HUVECs), rat aorta rings, and transgenic zebrafish...
November 30, 2018: International Journal of Molecular Sciences
Roee Birnbaum, Naama Yosha-Orpaz, Miri Yanoov-Sharav, Dvora Kidron, Hila Gur, Keren Yosovich, Tally Lerman-Sagie, Gustavo Malinger, Dorit Lev
Protein arginine methyltransferase 7 (PRMT7) is a member of a family of enzymes that catalyze the transfer of methyl groups from S-adenosyl-l-methionine to nitrogen atoms on arginine residues. Arginine methylation is involved in multiple biological processes, such as signal transduction, mRNA splicing, transcriptional control, DNA repair, and protein translocation. Currently, 10 patients have been described with mutations in PRMT7. The shared findings include: hypotonia, intellectual disability, short stature, brachydactyly, and mild dysmorphic features...
December 4, 2018: American Journal of Medical Genetics. Part A
Mu Wang, Wei Xia, Hongbin Liu, Fang Liu, Han Li, Huailong Chang, Jie Sun, Wenyu Liu, Xiaojie Sun, Yangqian Jiang, Hongxiu Liu, Chuansha Wu, Xinyun Pan, Yuanyuan Li, Weiqing Rang, Songfeng Lu, Shunqing Xu
Pregnant women are a unique group undergoing profound structural modifications in uterus, breast, adipose tissue and extracellular fluids. Amino acid metabolic stress is a unique physical process that occurs during pregnancy. Metals constitute a fundamental part of the maternal body and have a universal effect on amino acid metabolism. However, the exact interaction between metals and amino acid metabolism during pregnancy is unknown. The aim of the present study was to determine the correlations of metals with amino acid metabolic intermediates in the urine of 232 healthy pregnant women in their first, second and third trimesters during normal pregnancy...
November 1, 2018: Toxicology Research
Xin Yu, Liang Ge, Liang Niu, Xin Lian, Haichun Ma, Lei Pang
Nitric oxide synthases (NOSs) are a family of enzymes that are responsible for the synthesis of nitric oxide (NO) from the amino acid L-arginine in the body. Among the three key NOSs, the expression of inducible NOS (iNOS) can only be induced by inflammatory stimuli and contribute to the large amount of NO production. iNOS-derived NO plays an important role in various physiological and pathophysiological conditions, including the ischemic heart disease. Nowadays, the development of specific iNOS inhibitors and the availability of iNOS knockout mice have provided substantial evidence to support the role of iNOS/NO signaling in the myocardium...
2018: Oxidative Medicine and Cellular Longevity
Chen Lu, Xu Huang, Hong-Li Lu, Shao-Hua Liu, Jing-Yu Zang, Yu-Jia Li, Jie Chen, Wen-Xie Xu
AIM: To investigate the distribution and function of interstitial cells of Cajal (ICCs) and platelet-derived growth factor receptor-α positive (PDGFRα+ ) cells in the proximal and distal colon. METHODS: The comparison of colonic transit in the proximal and distal ends was performed by colonic migrating motor complexes (CMMCs). The tension of the colonic smooth muscle was examined by smooth muscle spontaneous contractile experiments with both ends of the smooth muscle strip tied with a silk thread...
November 28, 2018: World Journal of Gastroenterology: WJG
Eytan M Stein, Courtney D DiNardo, Amir T Fathi, Daniel A Pollyea, Richard M Stone, Jessica K Altman, Gail J Roboz, Manish R Patel, Robert Collins, Ian W Flinn, Mikkael A Sekeres, Anthony S Stein, Hagop M Kantarjian, Ross L Levine, Paresh Vyas, Kyle J MacBeth, Alessandra Tosolini, Jason VanOostendorp, Qiang Xu, Ira Gupta, Thomas Lila, Alberto Risueno, Katharine E Yen, Bin Wu, Eyal C Attar, Martin S Tallman, Stéphane de Botton
Approximately 8-19% of patients with acute myeloid leukemia (AML) have isocitrate dehydrogenase-2 ( IDH2 ) mutations, which occur at active site arginine residues, R140 and R172. IDH2 mutations produce an oncometabolite, 2-hydroxyglutarate (2-HG), that leads to DNA and histone hypermethylation and impaired hematopoietic differentiation. Enasidenib is an oral inhibitor of mutant-IDH2 proteins. This first-in-human phase I/II study evaluated enasidenib doses of 50-650 mg/day, administered in continuous 28-day cycles, in patients with mutant- IDH2 hematologic malignancies (ClinicalTrials...
December 3, 2018: Blood
Daniel Tomé
Glutamate (Glu), either as one of the amino acids of protein or in free form, constitutes up to 8-10% of amino acid content in the human diet, with an intake of about 10-20 g/day in adults. In the intestine, postprandial luminal Glu concentrations can be of the order of mM and result in a high intra-mucosal Glu concentration. Glu absorbed from the intestinal lumen is for a large part metabolized by enterocytes in various pathways, including the production of energy to support intestinal motility and functions...
2018: Annals of Nutrition & Metabolism
Mingyu He, Luyao Sun, Xiaoling Fu, Sean P McDonough, Chih-Chang Chu
The objective of this study is to design a new family of biodegradable synthetic polymeric biomaterials for providing a tunable inhibition of macrophage's nitric oxide synthase (NOS) pathway. L-Arginine (Arg) is the common substrate for NOS and arginase. Both two metabolic pathways participate in the wound healing process. An impaired wound healing, such as diabetic or other chronic wounds is usually associated with an overproduction of NO by macrophages via the NOS pathway. In this study, a new family of L-nitroarginine (NOArg) based polyester amide (NOArg-PEA) and NOArg-Arg PEA copolymers (co-PEA) were designed and synthesized with different composition ratios...
November 30, 2018: Acta Biomaterialia
Mitchell S Buckley, Jeffrey F Barletta, Pamela L Smithburger, John J Radosevich, Sandra L Kane-Gill
Shock syndromes are associated with unacceptably high rates of mortality in critically ill patients despite advances in therapeutic options. Vasodilatory shock is the most common type encountered in the intensive care unit. It is manifested by cardiovascular failure, peripheral vasodilatation, and arterial hypotension leading to tissue hypoperfusion and organ failure. Hemodynamic support is typically initiated with fluid resuscitation strategies and administration of adrenergic vasopressor agents in fluid nonresponsive patients to restore arterial pressure with subsequent adequate organ reperfusion...
December 1, 2018: Pharmacotherapy
Teng Xu, Cong Lu, Li Feng, Lin-Xi Fan, Jing Sun, Bei Fan, Qiong Wang, Yan Wang, Xin-Min Liu, Feng-Zhong Wang
The pathophysiological mechanism of depression is complex and its etiology remains unclear. Metabolomics can be used to monitor multiple metabolic pathways simultaneously, thereby investigating the mechanisms of depression onset and its regulation. Therefore, we studied the metabolic profile of urine samples from a rat model of depression for identifying potential metabolic biomarkers associated with depression. The depression model of rats was induced by 14-d simulated microgravity (SMG) treatment. Principal component analysis and orthogonal partial least squares discriminate analysis were performed to classify and identify the differences of endogenous metabolites in urine between the normal and depressed rats...
November 26, 2018: Journal of Pharmaceutical and Biomedical Analysis
Zsofia Kutil, Lubica Skultetyova, David Rauh, Marat Meleshin, Ivan Snajdr, Zora Novakova, Jana Mikesova, Jiri Pavlicek, Martin Hadzima, Petra Baranova, Barbora Havlinova, Pavel Majer, Mike Schutkowski, Cyril Barinka
Histone deacetylase 6 (HDAC6) is a multidomain cytosolic hydrolase acting mostly on nonhistone protein substrates. Investigations of the substrate specificity of HDAC6 are confounded by the presence of 2 catalytically active deacetylase domains (DD1 and DD2). In this study, acetylome peptide microarrays and peptide libraries were used to map the substrate specificity of DD1 and DD2 of human HDAC6. The results show that DD1 is solely responsible for the deacetylation of substrates harboring the acetyllysine at their C terminus, whereas DD2 exclusively deacetylates peptides with an internal acetyllysine residue...
November 29, 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
Chao-Wu Xiao, Carla Wood, Jesse Bertinato
L-Lysine (Lys) is a popular additive in foods, but the physiological effects of excess Lys supplementation are poorly understood and upper limits of safe intake have not been established. The objectives of this study were to examine the effects of dietary supplementation with increasing amounts of Lys on body weight (BW), food intake, and various blood hematological and biochemical parameters in rats. Male Sprague-Dawley rats at 10 weeks of age were assigned to ten diet groups (eight rats/group) and fed diets containing either 7% or 20% casein and supplemented with either 0% (Control), 1...
November 28, 2018: Molecular Biology Reports
Yanan Peng, Xiaoxiao Song, Tao Deng, Jie Zhang, Dawei Deng
Semiconductor quantum dots have attracted increasing attention, owing to their unique optical and electrical properties compared to traditional organic fluorescent dyes. However, one of the main obstacles impeding their biological applications is their biocompatibility. Hence, in this work, for achieving biocompatible quantum dots, oil-soluble ZnAgInSe/ZnS quantum dots without highly toxic heavy metals were selected, and four kinds of biocompatible thiols (dihydrolipoic acid, L-cysteine, N-acetyl-L-cysteine and glutathione) were explored as their water transfer agents...
April 1, 2019: Journal of Nanoscience and Nanotechnology
Alicia Forcada-Nadal, José Luis Llácer, Asunción Contreras, Clara Marco-Marín, Vicente Rubio
PII , a homotrimeric very ancient and highly widespread (bacteria, archaea, plants) key sensor-transducer protein, conveys signals of abundance or poorness of carbon, energy and usable nitrogen, converting these signals into changes in the activities of channels, enzymes, or of gene expression. PII sensing is mediated by the PII allosteric effectors ATP, ADP (and, in some organisms, AMP), 2-oxoglutarate (2OG; it reflects carbon abundance and nitrogen scarcity) and, in many plants, L-glutamine. Cyanobacteria have been crucial for clarification of the structural bases of PII function and regulation...
2018: Frontiers in Molecular Biosciences
Mohammad Khabbaz Shirazi, Asaad Azarnezhad, Mohammad Foad Abazari, Mansour Poorebrahim, Pegah Ghoraeian, Nima Sanadgol, Hanieh Bokharaie, Sahar Heydari, Amin Abbasi, Sahra Kabiri, Maryam Nouri Aleagha, Seyed Ehsan Enderami, Amir Savar Dashtaki, Hassan Askari
The interplay between H2 S and nitric oxide (NO) is thought to contribute to renal functions. The current study was designed to assess the role of NO in mediating the renoprotective effects of hydrogen sulfide in the 5/6 nephrectomy (5/6 Nx) animal model. Forty rats were randomly assigned to 5 experimental groups: (a) Sham; (b) 5/6 Nx; (c) 5/6Nx+sodium hydrosulfide-a donor of H 2 S, (5/6Nx+sodium hydrosulfide [NaHS]); (d) 5/6Nx+NaHS+ L-NAME (a nonspecific nitric oxide synthase [NOS] inhibitor); (e) 5/6Nx+NaHS+aminoguanidine (a selective inhibitor of inducible NOS [iNOS])...
November 27, 2018: Journal of Cellular Physiology
C Jeganathan, K Thamaraiselvi, T C Sabari Girisun
Enhanced production and growth of Halobacterium salinarum are achieved by direct supplement of essential amino acids in the modified nutrient culture medium. As arginine (R) and aspartate (D) are the main amino acid sources for producing bacteriorhodopsin efficiently from Halobacterium salinarum, both individual and combined effects of these two amino acids (in different compositions) in the basal medium were studied. The BR production is enhanced by 83% on the eighth day (saturated) for all individual and combined amino acid supplements...
November 26, 2018: Extremophiles: Life Under Extreme Conditions
Renke Maas, Maren Mieth, Stephanie I Titze, Silvia Hübner, Martin F Fromm, Jan T Kielstein, Matthias Schmid, Anna Köttgen, Florian Kronenberg, Vera Krane, Birgit Hausknecht, Kai-Uwe Eckardt, Markus P Schneider
Background: Elevated plasma concentrations of symmetric and asymmetric dimethylarginine (SDMA and ADMA, respectively) and a lower plasma concentration of the structurally related homoarginine are commonly observed in patients with chronic kidney disease (CKD) and independently predict total mortality as well as progression of renal disease. We aimed to identify drugs that may alter this adverse metabolite pattern in a favourable fashion. Methods: Plasma ADMA, SDMA, homoarginine and l-arginine were determined by liquid chromatography-tandem mass spectrometry in 4756 CKD patients ages 18-74 years with an estimated glomerular filtration rate (eGFR) of 30-60 mL/min/1...
November 23, 2018: Nephrology, Dialysis, Transplantation
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