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https://www.readbyqxmd.com/read/29134505/upregulated-sphingosine-1-phosphate-receptor-1-expression-in-human-and-murine-atherosclerotic-plaques
#1
Hui Liu, Hongjun Jin, Junbin Han, Xuyi Yue, Hao Yang, Mohamed A Zayed, Robert J Gropler, Zhude Tu
PURPOSE: Dysregulation of sphingosine 1-phosphate receptor 1 (S1PR1) signaling contributes to inflammation-related pathophysiological changes in cardiovascular diseases including atherosclerosis (AS). S1PR1-targeting compounds significantly reduce lesion size in murine models of AS. Therefore, characterization of S1PR1 expression in vitro and in vivo in atherosclerotic plaque could enable mechanistic studies and inform S1PR1 targeted therapies. PROCEDURES: H&E staining and immunostaining studies were performed on variably diseased human femoral endarterectomy plaque specimens, as well as mouse aortic sections from ApoE(-/-) mice maintained on a high-fat diet (AS mice)...
November 13, 2017: Molecular Imaging and Biology: MIB: the Official Publication of the Academy of Molecular Imaging
https://www.readbyqxmd.com/read/29097913/pet-imaging-of-fshr-expression-in-tumors-with-68-ga-labeled-fsh1-peptide
#2
Donghui Pan, Guifeng Liu, Yuping Xu, Yanting Wang, Yuanyuan Yue, Lizhen Wang, Junjie Yan, Xinyu Wang, Runlin Yang, Min Yang
FSHR is an appealing target for cancer theranostics. Radiolabeled FSH1 and its derivatives have shown potential to in vivo detect FSHR expression. However, moderate labeling yields (~50% nondecay-corrected) may partially limit their wide use. (68)Ga is an excellent PET nuclide due to availability, nearly quantitative reaction, and short physical half-life. In this study, (68)Ga labeled FSH1 peptide was developed for imaging of FSHR in cancers. In vitro studies and MicroPET imaging were performed in PC-3 prostate tumor model...
2017: Contrast Media & Molecular Imaging
https://www.readbyqxmd.com/read/29097911/micropet-ct-imaging-of-axl-downregulation-by-hsp90-inhibition-in-triple-negative-breast-cancer
#3
Wanqin Wang, Jun Zhao, Xiaoxia Wen, Curtis Chun-Jen Lin, Junjie Li, Qian Huang, Yongqiang Yu, Shiaw-Yih Lin, Chun Li
AXL receptor tyrosine kinase is overexpressed in a number of solid tumor types including triple-negative breast cancer (TNBC). AXL is considered an important regulator of epithelial-to-mesenchymal transition (EMT) and a potential therapeutic target for TNBC. In this work, we used microPET/CT with (64)Cu-labeled anti-human AXL antibody ((64)Cu-anti-hAXL) to noninvasively interrogate the degradation of AXL in vivo in response to 17-allylamino-17-demethoxygeldanamycin (17-AAG), a potent inhibitor of HSP90. 17-AAG treatment caused significant decline in AXL expression in orthotopic TNBC MDA-MB-231 tumors, inhibited EMT, and delayed tumor growth in vivo, resulting in significant reduction in tumor uptake of (64)Cu-anti-hAXL as clearly visualized by microPET/CT...
2017: Contrast Media & Molecular Imaging
https://www.readbyqxmd.com/read/29080056/oxygen-15-labeled-co2-o2-and-co-pet-in-small-animals-evaluation-using-a-3d-mode-micropet-scanner-and-impact-of-reconstruction-algorithms
#4
Genki Horitsugi, Tadashi Watabe, Yasukazu Kanai, Hayato Ikeda, Hiroki Kato, Sadahiro Naka, Mana Ishibashi, Keiko Matsunaga, Kayako Isohashi, Eku Shimosegawa, Jun Hatazawa
BACKGROUND: Positron emission tomography (PET) studies using (15)O-labeled CO2, O2, and CO have been used in humans to evaluate cerebral blood flow (CBF), the cerebral oxygen extraction fraction (OEF), and the cerebral metabolic rate of oxygen (CMRO2) and cerebral blood volume (CBV), respectively. In preclinical studies, however, PET studies using (15)O-labeled gases are not widely performed because of the technical difficulties associated with handling labeled gases with a short half-life...
October 27, 2017: EJNMMI Research
https://www.readbyqxmd.com/read/29077399/last-step-pd-mediated-11-c-co-labeling-of-a-moxestrol-conjugated-o-iodobenzyl-alcohol-from-model-experiments-to-in-vivo-positron-emission-tomography-studies
#5
Thomas Cornilleau, Mette Simonsen, Maylou Vang, Nada Taib-Maamar, Jean Dessolin, Hélène Audrain, Philippe Hermange, Eric Fouquet
The fast, efficient, and functional group tolerant last-step radiolabeling of bioconjugates is crucial for positron emission tomography (PET) applications. In this context, o-iodobenzyl alcohol based structures were identified as ideal tags for an easy Pd-catalyzed carbonylation after bioconjugation, and a moxestrol-conjugated precursor was chosen as the model compound for the further studies. Despite scale and time constraints, conditions developed with [(12)C]CO and [(13)C]CO were easily transferred to the (11)C isotope, and the desired radioactive product was obtained in amounts up to 740 MBq with radiochemical purities higher than 99%...
October 27, 2017: Bioconjugate Chemistry
https://www.readbyqxmd.com/read/29055123/disruption-of-behavior-and-brain-metabolism-in-artificially-reared-rats
#6
Elsa L Aguirre-Benítez, Mercedes G Porras, Leticia Parra, Jacquelina González-Ríos, Dafne F Garduño-Torres, Damaris Albores-García, Arturo Avendaño, Miguel A Ávila-Rodríguez, Angel I Melo, Ismael Jiménez-Estrada, Ma Eugenia Mendoza-Garrido, César Toriz, Daniel Diaz, Elizabeth Ibarra-Coronado, Karina Mendoza-Ángeles, Jesús Hernández-Falcón
Early adverse life stress has been associated to behavioral disorders that can manifest as inappropriate or aggressive responses to social challenges. In this study, we analyzed the effects of artificial rearing on the open field and burial behavioral tests and on GFAP, c-Fos immunoreactivity, and glucose metabolism measured in anxiety-related brain areas. Artificial rearing of male rats was performed by supplying artificial milk through a cheek cannula and tactile stimulation, mimicking the mother's licking to rat pups from the fourth postnatal day until weaning...
December 2017: Developmental Neurobiology
https://www.readbyqxmd.com/read/29046888/imaging-sex-differences-in-regional-brain-metabolism-during-acute-opioid-withdrawal
#7
Giovanni C Santoro, Joseph Carrion, Stephen L Dewey
The rate of opioid overdose continues to rise, necessitating improved treatment options. Current therapeutic approaches rely on administration of either a blocking agent, such as naloxone, or chronic treatment with replacement drugs, including methadone and/or buprenorphine. Recent findings suggest that males and females respond to these treatments uniquely. In an effort to better understand this sex-specific variation in treatment efficacy, we investigated the effects of acute opioid withdrawal in male and female rats using 18FDG and microPET...
April 2017: Journal of Alcoholism and Drug Dependence
https://www.readbyqxmd.com/read/29031997/a-six-year-longitudinal-pet-study-of-11-c-dtbz-binding-to-the-vmat2-in-monkey-brain
#8
Michael R Kilbourn, Robert A Koeppe
INTRODUCTION: The longitudinal reproducibility of in vivo binding potential measures for [(11)C]dihydrotetrabenazine ([(11)C]DTBZ) binding to the vesicular monoamine transporter 2 (VMAT2) site in primate brain was examined using a unique dataset of repeated control PET imaging studies. METHODS: Forty-one dynamic [(11)C]DTBZ PET studies were completed in a single rhesus monkey. Imaging equipment (microPET P4), personnel, radiotracer characteristics (injected mass amounts, molar activity) and image data analysis (BPND-Logan) were consistent throughout the entire sequence of PET studies...
September 5, 2017: Nuclear Medicine and Biology
https://www.readbyqxmd.com/read/28984069/assessment-of-radiopharmaceutical-retention-for-vascular-access-ports-using-positron-emission-tomography-imaging
#9
Michael S Gossman, Huaiyu Zheng, John G Evans, Junling Li, Chin K Ng
PURPOSE: The purpose of this study was to resolve the issue of whether various generations of CR Bard peripheral vascular access ports and catheters are prone to retain PET radiopharmaceuticals. The study evaluates the residual radioactivity remaining following injection for two PET radiopharmaceuticals currently used extensively in the clinic, FDG and Na(18) F. METHODS: FDG was purchased from a local cyclotron facility and Na(18) F was prepared in-house. Three generations of currently marketed vascular access ports were tested...
October 5, 2017: Journal of Applied Clinical Medical Physics
https://www.readbyqxmd.com/read/28929339/intranasal-insulin-ameliorates-cerebral-hypometabolism-neuronal-loss-and-astrogliosis-in-streptozotocin-induced-alzheimer-s-rat-model
#10
Yanxing Chen, Zhangyu Guo, Yan-Fang Mao, Tingting Zheng, Baorong Zhang
Intracerebroventricular injection of streptozotocin (ICV-STZ) in rodents leads to cognitive impairments and several pathological changes like Alzheimer's disease (AD). However, there is hardly any research about the effect of ICV-STZ on regional cerebral glucose metabolism in rodents. Previous studies have demonstrated that intranasal insulin improves cognition in AD patients. However, the underlying mechanism remains elusive. Here, we treated the ICV-STZ rats with daily intranasal delivery of insulin (2 U/day) for 6 consecutive weeks, then monitored (18)F-fluorodeoxyglucose ((18)F-FDG) uptake using a high-resolution small-animal positron emission tomography (microPET) and studied the expression of neuronal nuclei (NeuN) and glial fibrillary acidic protein (GFAP) using immunohistochemical staining...
September 19, 2017: Neurotoxicity Research
https://www.readbyqxmd.com/read/28917111/-11-c-pf-3274167-as-a-pet-radiotracer-of-oxytocin-receptors-radiosynthesis-and-evaluation-in-rat-brain
#11
Benjamin Vidal, Iuliia A Karpenko, François Liger, Sylvain Fieux, Caroline Bouillot, Thierry Billard, Marcel Hibert, Luc Zimmer
INTRODUCTION: Oxytocin plays a major role in the regulation of social interactions in mammals by interacting with the oxytocin receptor (OTR) expressed in the brain. Furthermore, the oxytocin system appears as a possible therapeutic target in autism spectrum disorders and other psychiatric troubles, justifying current pharmacological researches. Since no specific PET radioligand is currently available to image OTR in the brain, the aim of this study was to radiolabel the specific OTR antagonist PF-3274167 and to evaluate [(11)C]PF-3274167 as a potential PET tracer for OTR in rat brains...
August 2, 2017: Nuclear Medicine and Biology
https://www.readbyqxmd.com/read/28916621/-64-cu-labeled-repebody-molecules-for-imaging-of-epidermal-growth-factor-receptor-expressing-tumors
#12
Ayoung Pyo, Misun Yun, Hyeon-Sik Kim, Tae-Yoon Kim, Joong-Jae Lee, Jung Young Kim, Sunwoo Lee, Seong Young Kwon, Hee-Seung Bom, Hak-Sung Kim, Dong-Yeon Kim, Jung-Joon Min
The epidermal growth factor receptor (EGFR) is a member of the erbB family of receptors and is overexpressed in many tumor types. A repebody is a newly designed non-antibody protein scaffold for tumor targeting that contains leucine-rich repeat modules. In this study, three (64)Cu-labeled anti-EGFR repebodies with different chelators were synthesized, and their biological characteristics were assessed in cultured cells and tumor-bearing mice. Methods: Repebodies (rEgAs) were synthesized with the chelators p-SCN-Bn-NOTA, DOTA-NHS ester, or p-SCN-Bn-DTPA in 1...
September 15, 2017: Journal of Nuclear Medicine: Official Publication, Society of Nuclear Medicine
https://www.readbyqxmd.com/read/28877656/longitudinal-evaluation-of-myocardial-fatty-acid-and-glucose-metabolism-in-fasted-and-nonfasted-spontaneously-hypertensive-rats-using-micropet-ct
#13
Jennifer S Huber, Andrew M Hernandez, Mustafa Janabi, James P O'Neil, Kathleen M Brennan, Stephanie T Murphy, Youngho Seo, Grant T Gullberg
Using longitudinal micro positron emission tomography (microPET)/computed tomography (CT) studies, we quantified changes in myocardial metabolism and perfusion in spontaneously hypertensive rats (SHRs), a model of left ventricular hypertrophy (LVH). Fatty acid and glucose metabolism were quantified in the hearts of SHRs and Wistar-Kyoto (WKY) normotensive rats using long-chain fatty acid analog (18)F-fluoro-6-thia heptadecanoic acid ((18)F-FTHA) and glucose analog (18)F-fluorodeoxyglucose ((18)F-FDG) under normal or fasting conditions...
January 2017: Molecular Imaging
https://www.readbyqxmd.com/read/28852941/monitoring-of-cardiac-remodeling-in-a-mouse-model-of-pressure-overload-left-ventricular-hypertrophy-with-18-f-fdg-micropet
#14
Andrei Todica, Nick L Beetz, Lisa Günther, Mathias J Zacherl, Ulrich Grabmaier, Bruno Huber, Peter Bartenstein, Stefan Brunner, Sebastian Lehner
PURPOSE: This study aims to analyze the left ventricular function parameters, scar load, and hypertrophy in a mouse model of pressure-overload left ventricular (LV) hypertrophy over the course of 8 weeks using 2-deoxy-2-[(18)F]fluoro-D-glucose ([(18)F]FDG) micro-positron emission tomography (microPET) imaging. PROCEDURES: LV hypertrophy was induced in C57BL/6 mice by transverse aortic constriction (TAC). Myocardial hypertrophy developed after 2-4 weeks. ECG-gated microPET scans with [(18)F]FDG were performed 4 and 8 weeks after surgery...
August 29, 2017: Molecular Imaging and Biology: MIB: the Official Publication of the Academy of Molecular Imaging
https://www.readbyqxmd.com/read/28822038/translocator-protein-pet-imaging-in-a-preclinical-prostate-cancer-model
#15
Mohammed N Tantawy, H Charles Manning, Todd E Peterson, Daniel C Colvin, John C Gore, Wenfu Lu, Zhenbang Chen, C Chad Quarles
PURPOSE: The identification and targeting of biomarkers specific to prostate cancer (PCa) could improve its detection. Given the high expression of translocator protein (TSPO) in PCa, we investigated the use of [(18)F]VUIIS1008 (a novel TSPO-targeting radioligand) coupled with positron emission tomography (PET) to identify PCa in mice and to characterize their TSPO uptake. PROCEDURES: Pten(pc-/-), Trp53(pc-/-) prostate cancer-bearing mice (n = 9, 4-6 months old) were imaged in a 7T MRI scanner for lesion localization...
August 18, 2017: Molecular Imaging and Biology: MIB: the Official Publication of the Academy of Molecular Imaging
https://www.readbyqxmd.com/read/28791653/selective-targeting-of-melanoma-using-n-2-diethylaminoethyl-4-18-f-fluoroethoxy-benzamide-4-18-f-febza-a-novel-pet-imaging-probe
#16
Pradeep K Garg, Rachid Nazih, Yanjun Wu, Vladimir P Grinevich, Sudha Garg
BACKGROUND: The purpose of this study was to develop a positron emission tomography (PET) imaging probe that is easy to synthesize and selectively targets melanoma in vivo. Herein, we report the synthesis and preclinical evaluation of N-(2-diethylaminoethyl) 4-[(18)F]Fluoroethoxy benzamide (4-[(18)F]FEBZA). A one-step synthesis was developed to prepare 4-[(18)F]FEBZA in high radiochemical yields and specific activity. The binding affinity, the in vitro binding, and internalization studies were performed using B16F1 melanoma cell line...
August 8, 2017: EJNMMI Research
https://www.readbyqxmd.com/read/28654383/brain-and-brown-adipose-tissue-metabolism-in-transgenic-tg2576-mice-models-of-alzheimer-disease-assessed-using-18-f-fdg-pet-imaging
#17
Robert A Coleman, Christopher Liang, Rima Patel, Sarah Ali, Jogeshwar Mukherjee
OBJECTIVE: Imaging animal models of Alzheimer disease (AD) is useful for the development of therapeutic drugs and understanding AD. Transgenic Swedish hAPPswe Tg2576 mice are a good model of β-amyloid plaques. We report (18)F-fluoro-2-deoxyglucose ((18)F-FDG) positron emission tomography (PET) imaging of brain and intrascapular brown adipose tissue (IBAT) in transgenic mice 2576 (Tg2576) and wild-type (WT) mice. METHODS: Transgenic Tg2576 mice and WT mice, >18 months were injected intraperitonally with ≈ 25 to 30 MBq (18)F-FDG while awake...
January 1, 2017: Molecular Imaging
https://www.readbyqxmd.com/read/28654378/pet-study-of-sphingosine-1-phosphate-receptor-1-expression-in-response-to-vascular-inflammation-in-a-rat-model-of-carotid-injury
#18
Hui Liu, Hongjun Jin, Xuyi Yue, Junbin Han, Pamela Baum, Dana R Abendschein, Zhude Tu
Sphingosine-1-phosphate receptor (S1PR) activation plays a key role in vascular inflammatory response. Here, we report in vivo validation of [(11)C]TZ3321, a potent S1PR1 radioligand, for imaging vascular inflammation in a rat model of carotid injury. The right common carotid artery of male adult Sprague-Dawley rats was injured by balloon overinflation that denuded the endothelium and distended the vessel wall. Animals received a 60-minute micro-positron emission tomography (micro PET) scan with [(11)C]TZ3321 at 72 hours after injury...
January 1, 2017: Molecular Imaging
https://www.readbyqxmd.com/read/28651055/pharmacokinetic-characterization-of-18-f-ucb-h-pet-radiopharmaceutical-in-the-rat-brain
#19
Guillaume Becker, Corentin Warnier, Maria Elisa Serrano, Mohamed Ali Bahri, Joël Mercier, Christian Lemaire, Eric Salmon, André Luxen, Alain Plenevaux
The synaptic vesicle glycoprotein 2A (SV2A), a protein essential to the proper nervous system function, is found in presynaptic vesicles. Thus, SV2A targeting, using dedicated radiotracers combined with positron emission tomography (PET), allows the assessment of synaptic density in the living brain. The first-in-class fluorinated SV2A specific radioligand, [(18)F]UCB-H, is now available at high activity through an efficient radiosynthesis compliant with current good manufacturing practices (cGMP). We report here a noninvasive method to quantify [(18)F]UCB-H binding in rat brain with microPET...
August 7, 2017: Molecular Pharmaceutics
https://www.readbyqxmd.com/read/28649566/-68-ga-188-re-complexed-cdtmp-trans-1-2-cyclohexyldinitrilotetraphosphonic-acid-as-a-theranostic-agent-for-skeletal-metastases
#20
Ambika P Jaswal, Virendra K Meena, Surbhi Prakash, Ankita Pandey, Baljinder Singh, Anil K Mishra, Puja P Hazari
OBJECTIVE: Metastasis of the osseous tissue is one of the frequent and severe aggravations as a result of several neoplastic conditions, such as metabolic disorders, infections, and cancer. The objective of this study was to evaluate the pertinence of [(68)Ga]-trans-1,2-cyclohexyldinitrilo tetramethylene phosphonic acid (CDTMP) as a potential bone imaging agent for positron emission tomography (PET) applications as well as to assess [(188)Re]-CDTMP for bone pain palliation in metastatic skeletal disorders...
2017: Frontiers in Medicine
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