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Eliisa Ollikainen, Riikka Tulamo, Salla Kaitainen, Petri Honkanen, Satu Lehti, Timo Liimatainen, Juha Hernesniemi, Mika Niemelä, Petri T Kovanen, Juhana Frösen
Saccular intracranial aneurysm (sIA) rupture is often fatal. Rupture-prone sIA walls are infiltrated by macrophages expressing hemoglobin-receptor CD163, suggesting a role for erythrocyte lysis in the degenerative remodeling predisposing to rupture. We therefore studied erythrocyte remnants in 16 unruptured and 20 ruptured sIA walls using histology and immunohistochemistry. Glycophorin A (GPA), an erythrocyte membrane protein, was present in 34/36 (94%) sIA walls and correlated with loss of αSMA+ cells, reflecting loss of mural smooth muscle cells ([SMCs]; r = -0...
July 17, 2018: Journal of Neuropathology and Experimental Neurology
Kai-Le Wang, Can Chen, Peng-Fei Shi, Jian-Hua Yu, Jun-Feng Tan, Shen-Xian Qian, Da-Quan Gao, Kuang Chen, Li-Rong Liu, Ya-Pin Xie, Ying Xu
OBJECTIVE: To investigate the prognostic value of morphology and Hans classification in diffuse large B cell lymphoma(DLBCL). METHODS: Clinical data of 249 patients diagnosed with DLBCL in our hospital and Hangzhou Xixi hospital during Jan 2006 to Dec 2016 were analyzed retrospectively. These patients were classified into 3 groups: immunoblastic variant(IB) group, centroblastic variant(CB) group and others group according to the cell morphology. And DLBCL was also divided into GCB(germinal center B-cell-like)or non-GCB(non-germinal center B-cell-like) group by analyzing the expression of CD10, BCL6 and MUM1 (GCB: CD10 + ,BCL6+- ,MUM1+- /CD10- ,BCL6+ ,MUM1- ;non-GCB:CD10- ,BCL6- ,MUM1+- /CD10- ,BCL6+ ,MUM1+ )...
August 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
Yan Wang, Chun Qiao, Rui Guo, Hui Wang, Hui Yang, Hai-Rong Qiu, Yu-Jie Wu, Jian-Fu Zhang, Rong Wang, Si-Xuan Qian, Jian-Yong Li
OBJECTIVE: To analyze the clinical and laboratory features of acute myeloid leukemia (AML) with cuplike nuclei morphology. METHODS: One hundred and seventy patients diagnosed with AML (M1andM2) between December 2009 and December 2016 were included in the study. Bone marrow smears were prepared for morphologic alanalysis, the immunophenotype was analyzed by flow cytometry and the RHG-banding was for conventional cytogenetic assay (CCA) ,gene mutation was detected by sequencing...
August 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
Jun Chen, Jingna Xun, Junyang Yang, Yongjia Ji, Li Liu, Tangkai Qi, Zhenyan Wang, Renfang Zhang, Yinzhong Shen, Rosalie Ponte, Vikram Mehraj, Jean-Pierre Routy, Hongzhou Lu
Background: Indoleamine 2, 3-dioxygenase (IDO) is an immunoregulatory enzyme that metabolises tryptophan to immunosuppressive kynurenines. We investigated whether IDO activity is associated with the size of HIV reservoirs. Methods: In this cross-sectional study, total HIV DNA in PBMCs from 127 HIV-infected patients receiving antiretroviral therapy (ART) was quantified. Tryptophan and kynurenine concentrations, as well as microbial translocation markers were measured in plasma samples...
August 10, 2018: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
Cecilia T Costiniuk, Syim Salahuddin, Omar Farnos, Ron Olivenstein, Amélie Pagliuzza, Marianna Orlova, Erwin Schurr, Christina De Castro, Jean Bourbeau, Jean-Pierre Routy, Petronela Ancuta, Nicolas Chomont, Mohammad-Ali Jenabian
BACKGROUND: The lungs were historically identified as one of the major anatomic sites for HIV replication in the pre-ART era. However, their contribution to HIV persistence in individuals under suppressive ART remains understudied. DESIGN: We assessed HIV persistence and comprehensively characterized pulmonary mucosal CD4 T-cells in HIV individuals receiving long-term suppressive ART versus uninfected participants. METHODS: Bronchoalveolar lavage (BAL), bronchial biopsies and matched peripheral blood were obtained from n=24 HIV-infected adults receiving long-term suppressive ART (median: 9 years) and n = 8 healthy volunteers without respiratory symptoms...
August 9, 2018: AIDS
Lotta Jansson, Kathleen Vrolix, Andrea Jahraus, Keith F Martin, David C Wraith
We have combined MHC binding assays with immunization and tolerance induction experiments in HLA-DR3 transgenic mice in order to design apitopes (Antigen Processing Independent epiTOPES) derived from TSHR for treatment of patients with Graves' disease (GD). A challenge model was created by using an adenovirus expressing part of the extracellular domain of the thyrotropin receptor (TSHR289). This model was used to test whether current drug treatments for GD would impact on effective antigen-specific immunotherapy using the apitope approach...
August 6, 2018: Endocrinology
Meizhu Xiao, Ying Feng, Guangming Cao, Chongdong Liu, Zhenyu Zhang
OBJECTIVE: To evaluate the potential effects of recombinant mycobacterium tuberculosis heat shock protein 70-formyl peptide receptor 1 (MtHSP70-FPR1) fusion protein on human monocyte-derived dendritic cell (moDC) maturation; cytotoxic T lymphocyte (CTL) responses to cervical cancer (CC) cells; and the roles of the p38 MAPK, ERK, and JNK pathways in its transition. METHODS: Monocytes were positively selected with a MACS column with antiCD14 antibody-conjugated microbeads from umbilical cord blood...
August 8, 2018: Biochemical and Biophysical Research Communications
Raquel Almansa, Silvia Martín, Marta Martin-Fernandez, María Heredia-Rodríguez, Esther Gómez-Sánchez, Marta Aragón, Cristina Andrés, Dolores Calvo, Jesus Rico-Feijoo, Maria Carmen Esteban-Velasco, Luis Mario Vaquero-Roncero, Alicia Ortega, Estefania Gómez-Pesquera, Mario Lorenzo-López, Iñigo López de Cenarruzabeitia, Diana Benavides, Jaime López-Sanchez, Cristina Doncel, Carmen González-Sanchez, Esther Zarca, Alberto Ríos-Llorente, Agustín Diaz, Elisa Sanchez-Barrado, Juan Beltran de Heredia, Jose Maria Calvo-Vecino, Luis Muñoz-Bellvís, Jose Ignacio Gomez-Herreras, César Aldecoa, Eduardo Tamayo, Jesus F Bermejo-Martin
Early recognition of sepsis is a key factor to improve survival to this disease in surgical patients, since it allows prompt control of the infectious source. Combining pro-inflammatory and immunosupression biomarkers could represent a good strategy to improve sepsis detection. Here we evaluated the combination of procalcitonin (PCT) with gene expression levels of HLA-DRA to detect sepsis in a cohort of 154 surgical patients (101 with sepsis and 53 with no infection). HLA-DRA expression was quantified using droplet digital PCR, a next-generation PCR technology...
August 10, 2018: Scientific Reports
Gunilla Enblad, Hannah Karlsson, Gustav Gammelgard, Jessica Wenthe, Tanja Lovgren, Rose-Marie Amini, Kristina I Wikstrom, Magnus Essand, Barbara Savoldo, Helene Hallbook, Martin Höglund, Gianpietro Dotti, Malcolm K Brenner, Hans Hagberg, Angelica Loskog
PURPOSE: CAR T cell therapy has been effective for patients with CD19+ B cell malignancies. Most studies have investigated second generation CARs with either CD28 or 4-1BB co-stimulatory domains in the CAR receptor. Here we describe the first clinical phase I/IIa trial using third generation CAR T cells targeting CD19 to evaluate safety and efficacy. EXPERIMENTAL DESIGN: Fifteen patients with B cell lymphoma or leukemia were treated with CAR T cells. The lymphoma patients received chemotherapy during CAR manufacture and eleven of fifteen were given low dose cyclophosphamide and fludarabine conditioning prior to CAR infusion...
August 10, 2018: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
Nufar Marcus, Tali Stauber, Atar Lev, Amos J Simon, Jerry Stein, Arnon Broides, Ido Somekh, Shlomo Almashanu, Raz Somech
Newborn screening (NBS) programs for severe combined immunodeficiency (SCID), using the TREC-based assay, have enabled early diagnosis, prompt treatment, and eventually changed the natural history of affected infants. Nevertheless, it was believed that some affected infants with residual T cell, such as patients with MHC II deficiency, will be misdiagnosed by this assay. A full immune workup and genetic analysis using direct Sanger sequencing and whole exome sequencing have been performed to a patient that was identified by the Israeli NBS program for SCID...
August 6, 2018: Immunologic Research
Julie Alingrin, Benjamin Coiffard, Julien Textoris, Corine Nicolino-Brunet, Morgane Gossez, Pierre-André Jarrot, Françoise Dignat-George, Guillaume Monneret, Pascal Alexandre Thomas, Marc Leone, Martine Reynaud-Gaubert, Laurent Papazian
BACKGROUND: Immunosuppressive strategy targets mainly adaptive immunity after solid organ transplantation. We assessed the influence of early post-operative sepsis on T cell and monocyte reconstitution in anti-thymocyte globulin (ATG)-treated lung transplant recipients. METHODS: We retrospectively included recipients who underwent a first lung transplant at our Lung Transplant Center (Marseille, France) between July 2011 and February 2013. Peripheral blood T-lymphocyte subset counts and monocyte HLA-DR (mHLA-DR) expression routinely performed by flow cytometry within 60 days post-transplant were analyzed...
August 3, 2018: Transplant Immunology
Xiaoliang Wu, Jinchao Hou, Hui Li, Guohao Xie, Xiaolin Zhang, Jungang Zheng, Jiang Wang, Feng Gao, Yongming Yao, Hong Liu, Xiangming Fang
INTRODUCTION: The aim of this study was to investigate the correlation between plasma Sphingosine-1-phosphate (S1P) and ceramide concentrations in sepsis, and the possible mechanisms for altered expression. METHODS: Plasma S1P and ceramide concentrations were measured by HPLC-ESI-MS/MS. HLA-DR expression on peripheral blood mononuclear cells was examined by flow cytometry. Platelet sphingosine kinases 1/2 (SphK1/2) mRNA expression, protein content, and enzyme activities were determined by qRT-PCR, western blot, and commercial enzyme assay kits respectively...
August 3, 2018: Shock
Tanongsak Laowanitwattana, Sirinda Aungsuchawan, Suteera Narakornsak, Runchana Markmee, Waleephan Tancharoen, Junjira Keawdee, Nonglak Boonma, Witoon Tasuya, Lamaiporn Peerapapong, Nathaporn Pangjaidee, Peeraphan Pothacharoen
Osteoporosis is a bone degenerative disease characterized by a decrease in bone strength and an alteration in the osseous micro-architecture causing an increase in the risk of fractures. These diseases usually happen in post-menopausal women and elderly men. The most common treatment involves anti-resorptive agent drugs. However, the inhibition of bone resorption alone is not adequate for recovery in patients at the severe stage of osteoporosis who already have a fracture. Therefore, the combination of utilizing osteoblast micro mimetic scaffold in cultivation with the stimulation of osteoblastic differentiations to regain bone formation is a treatment strategy of considerable interest...
August 2, 2018: Acta Histochemica
Norman J Galbraith, Stephen J O'Brien, Samuel P Walker, Sarah A Gardner, Hiram C Polk, Stephen L Barnes
BACKGROUND: Severe injury can lead to immune dysfunction and predispose patients to infection and death. Micro-RNAs regulate gene expression and may act as biomarkers for susceptibility to infection. The aim of this study was to examine the temporal and differential expression of previously identified dysregulated micro-RNAs in patients with severe injury. METHODS: Fourteen severely injured patients requiring transfusion were enrolled prospectively in this study approved by our institutional review board...
August 1, 2018: Surgery
Zhu-Xi Yu, Xian-Cheng Chen, Bei-Yuan Zhang, Ning Liu, Qin Gu
Multidrug-resistant (MDR) bacterial infection is a common complication of severe acute pancreatitis (SAP). This study aimed to explore the association between human leukocyte antigen-antigen D-related (HLA-DR) expression and multidrug-resistant infection in patients with SAP. A total of 24 SAP patients who were admitted to Nanjing Drum Tower Hospital between May 2015 and December 2016 were enrolled in the study. The percentages of CD4+, CD8+, natural killer (NK), and HLA-DR (CD14+) cells and the CD4+/CD8+ cell ratio on days 1,7,14, and 28 after admission were determined by flow cytometry...
June 2018: Current medical science
Kathryn E Hally, Anne C La Flamme, Scott A Harding, Peter D Larsen
Objectives: Platelets are important regulators of vascular thrombosis and inflammation and are known to express Toll-like receptors (TLRs). Through TLRs, platelets mediate a number of responses by interacting with leucocytes. Here, we report the extent to which platelets modulate in vitro peripheral blood mononuclear cells (PBMCs) and granulocyte responses to TLR4, TLR2/1 and TLR2/6 stimulation in healthy subjects. Methods: Peripheral blood mononuclear cells and granulocytes from 10 healthy volunteers were cultured alone or cocultured with platelets...
2018: Clinical & Translational Immunology
Emma M Pinder, Anthony J Rostron, Thomas P Hellyer, Marie-Helene Ruchaud-Sparagano, Jonathan Scott, James G Macfarlane, Sarah Wiscombe, John D Widdrington, Alistair I Roy, Vanessa C Linnett, Simon V Baudouin, Stephen E Wright, Thomas Chadwick, Tony Fouweather, Jatinder K Juss, Edwin R Chilvers, Susan A Bowett, Jennie Parker, Daniel F McAuley, Andrew Conway Morris, A John Simpson
BACKGROUND: Critically ill patients with impaired neutrophil phagocytosis have significantly increased risk of nosocomial infection. Granulocyte-macrophage colony-stimulating factor (GM-CSF) improves phagocytosis by neutrophils ex vivo. This study tested the hypothesis that GM-CSF improves neutrophil phagocytosis in critically ill patients in whom phagocytosis is known to be impaired. METHODS: This was a multicentre, phase IIa randomised, placebo-controlled clinical trial...
July 31, 2018: Thorax
Fei Pei, Xiangdong Guan, Jianfeng Wu
Sepsis is a life-threatening organ dysfunction caused by host immune response to infection. In this regard, modifying host immune response may help to eliminate systemic infection and restore organ function. Thymosin alpha 1 (Tα1), acting as an immune modulator, exerts great biological influence in activating and restoring the dysregulated immune response for patients with sepsis. Areas covered: In this review, we summarize the clinical studies of Tα1 treatment alone and in combination with anti-inflammatory for patients with sepsis or septic shock...
July 2018: Expert Opinion on Biological Therapy
György Sinkovits, Ágnes Szilágyi, Péter Farkas, Dóra Inotai, Anikó Szilvási, Attila Tordai, Katalin Rázsó, Marienn Réti, Zoltán Prohászka
Background: The acquired form of idiopathic thrombotic thrombocytopenic purpura (TTP) is an autoimmune disease, in which the underlying deficiency of the ADAMTS13 protease is caused by autoantibodies, predominantly of the IgG isotype. Certain HLA-DR-DQ haplotypes were associated with the risk of developing TTP. Objectives: To investigate the development of the ADAMTS13-specific antibody response during the course of the disease, we analyzed the concentration, subclass distribution, and inhibitory potential of anti-ADAMTS13 IgG autoantibodies in samples of TTP patients drawn during the first acute phase, in remission, and during relapse...
2018: Frontiers in Immunology
Ju-Yang Jung, Bunsoon Choi, Hasan Md Sayeed, Chang-Hee Suh, Ye Won Kim, Hyoun-Ah Kim, Seonghyang Sohn
We examined the expression of human leukocyte antigen (HLA) and composition of differentiated T cells in the peripheral blood to understand the characteristics of the immune changes in patients with adult-onset Still's disease (AOSD). This study enrolled patients with AOSD (n = 14), patients with rheumatoid arthritis (RA, n = 20), and healthy controls (HC, n = 20). The percentage of surface-stained cells with HLA-DP, DQ, and DR alleles and the composition of differentiated T cells in peripheral blood leukocytes (PBLs) were evaluated by flow cytometry...
January 2018: International Journal of Immunopathology and Pharmacology
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