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Manabu Okada, Yoshihiko Watarai, Kenta Iwasaki, Kenta Futamura, Takayuki Yamamoto, Takahisa Hiramitsu, Makoto Tsujita, Norihiko Goto, Shunji Narumi, Asami Takeda, Takaaki Kobayashi
Recently, in vitro experiments have demonstrated that anti-blood group A/B antibody binding to endothelial cells induce a protective effect against antibody-mediated injury. This study aimed to clarify the potential clinical benefit of ABO incompatibility in donor-specific HLA antibody (DSA)-induced chronic antibody-mediated rejection (ABMR). We enrolled 215 ABO-incompatible renal transplant (ABO-I) and 467 ABO-identical/compatible renal transplant recipients (ABO-Id/C). The prevalence of de novo DSA production and incidence of biopsy-proven chronic ABMR were compared between the two groups...
December 12, 2018: Human Immunology
Kamal Abdolmohammadi, Fatemeh Dadgar Pakdel, Hamideh Aghaei, Sara Assadiasl, Yousef Fatahi, Negin Hosseini Rouzbahani, Alireza Rezaiemanesh, Masoud Soleimani, Lobat Tayebi, Mohammad Hossein Nicknam
Ankylosing spondylitis (AS) is an inflammatory rheumatoid disease categorized within spondyloarthropathies (SpA) and manifested by chronic spinal arthritis. Several innate and adaptive immune cells and secreted-mediators have been indicated to play a role in AS pathogenesis. Considering the limitations of current therapeutic approaches (NSAIDs, glucocorticoids, DMARDs and biologic drugs), finding new treatments with fewer side effects and high therapeutic potentials are required in AS. Mesenchymal stem cells (MSCs) with considerable immunomodulatory and regenerative properties could be able to attenuate the inflammatory responses and help tissue repair by cell-to-cell contact and secretion of soluble factors...
January 2019: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Robert B Lochhead, David Ordoñez, Sheila L Arvikar, John M Aversa, Luke S Oh, Benton Heyworth, Ruslan Sadreyev, Allen C Steere, Klemen Strle
Lyme arthritis (LA), a late disease manifestation of Borrelia burgdorferi infection, usually resolves with antibiotic therapy. However, some patients develop proliferative synovitis lasting months to several years after spirochetal killing, called post-infectious LA. In this study, we phenotyped hematopoietic and stromal cell populations in the synovial lesion ex vivo and used these findings to generate an in vitro model of LA using patient-derived fibroblast-like synoviocytes (FLS). Ex vivo analysis of synovial tissue revealed high abundance of IFNγ-producing T cells and NK cells...
December 14, 2018: Cellular Microbiology
Mirela Raos, Renata Zunec, Marko Mocibob, Koraljka Gojceta, Marija Lukic, Branka Golubic Cepulic
BACKGROUND: Alloimmunization is a known risk of transfusion therapy caused by exposure to foreign RBC antigens. However, alloimmunization is not observed in all transfused patients. Human leukocyte antigen (HLA) molecules may contribute to the recognition and presentation of foreign antigens and to the potency of immune responses that result in the production of antibodies. The aim of this study was to determine the association of HLA-DR and HLA-DQ polymorphisms with alloimunization to Fya antigen in Croatian patients...
December 12, 2018: Transfusion
Loic Raffray, Claude Giry, David Vandroux, Stéphanie Fayeulle, Marie-Pierre Moiton, Anne Gerber, Marie-Christine Jaffar-Bandjee, Philippe Gasque
Leptospirosis is a life-threatening zoonotic disease and it has been hypothesized that the innate immune system fails to control the infection through ill-characterized mechanisms. The aim of this observational study was to better evaluate the activation processes of monocytes at the early stage of the disease. Blood samples were taken from healthy donors (n = 37) and patients hospitalized for either non-severe (n = 25) or severe (n = 32) leptospirosis. Monocyte cell counts and phenotypes were assessed by flow cytometry...
December 12, 2018: Medical Microbiology and Immunology
Zhuxi Yu, Beiyuan Zhang, Ying Xu, Yingying Hao, Jian Tang, Wenkui Yu, Qin Gu
OBJECTIVE: To analyze the clinical characteristics of bloodstream infection in patients with immune function inhibition. METHODS: A retrospective analysis was conducted. 234 patients with bloodstream infection admitted to intensive care unit (ICU) of the Affiliated Drum Tower Hospital of Nanjing University Medical School from August 1st in 2015 to December 31st in 2017 were enrolled. According to the immune function on the day of bloodstream infection, they were divided into normal immune function group [human leukocyte antigen DR (HLA-DR) > 30%, n = 144] and immunosuppression group (HLA-DR ≤ 30%, n = 90)...
November 2018: Zhonghua Wei Zhong Bing Ji Jiu Yi Xue
Hury Hellen Souza de Paula, Ana Cristina Garcia Ferreira, Diogo Gama Caetano, Edson Delatorre, Sylvia Lopes Maia Teixeira, Lara Esteves Coelho, Eduarda Grinsztejn João, Michelle Morata de Andrade, Sandra Wagner Cardoso, Beatriz Grinsztejn, Valdilea Gonçalves Veloso, Mariza Gonçalves Morgado, Monick Lindenmeyer Guimarães, Fernanda Heloise Côrtes
OBJECTIVES: To investigate the impact of early combined antiretroviral therapy (cART) on inflammation biomarkers and immune activation during acute and early chronic HIV-1 infection. METHODS: We included 12 acute (AHI), 11 early chronic (EcHI), and 18 late chronic HIV-1-infected (LcHI) individuals who were treated with cART and 18 HIV-1-uninfected (HIV-neg) individuals. Plasmatic levels of inflammation biomarkers, CD8+ CD38+ HLA-DR+ T cell frequencies, CD4 T cell counts, CD4/CD8 ratio, total HIV-1 DNA and plasmatic viral load were evaluated...
December 12, 2018: Retrovirology
Xiaosan Su, Yaodong Fan, Liu Yang, Jie Huang, Fei Qiao, Yu Fang, Jun Wang
BACKGROUND: Dexmedetomidine (DEX) has been reported to promote tumour metastases. However the underlying mechanisms remain unclear. The purpose of this study was to investigate whether DEX promotes tumour metastasis by inducing myeloid-derived suppressor cells (MDSC) in the context of surgery. METHODS: DEX was given to lung cancer patients and its effects on expansion of monocytic MDSC (M-MDSC) were studied in the context of surgery. Spontaneous tumour metastasis was induced in C57BL/6 mice and the effects of DEX on M-MDSC expansion and metastasis formation were assessed...
December 11, 2018: Journal of Translational Medicine
Catalina Burbano, Jose A Gomez-Puerta, Carlos Muñoz-Vahos, Adriana Vanegas-García, Mauricio Rojas, Gloria Vásquez, Diana Castaño
Non-classical monocytes infiltrate the kidney parenchyma and participate in tissue damage in patients with lupus nephritis (LN). Circulating microparticles (MPs) seem to play critical roles in the activation of monocytes in systemic lupus erythematosus (SLE) patients. This study aims to characterize the phenotypes of MPs and monocyte subsets in LN patients and to determine their potential to discriminate between SLE patients with and without LN. Blood and urine samples from SLE patients were collected. In monocyte subsets from whole blood samples several phenotypic markers were evaluated...
December 10, 2018: European Journal of Immunology
Laurence C Menard, Paul Fischer, Bijal Kakrecha, Peter S Linsley, Erik Wambre, Maochang C Liu, Blake J Rust, Deborah Lee, Becky Penhallow, Nataly Manjarrez Orduno, Steven G Nadler
Checkpoint inhibitors target the inhibitory receptors expressed by tumor-infiltrating T cells in order to reinvigorate an anti-tumor immune response. Therefore, understanding T cell composition and phenotype in human tumors is crucial. We analyzed by flow cytometry tumor-infiltrating lymphocytes (TILs) from two independent cohorts of patients with different cancer types, including RCC, lung, and colon cancer. In healthy donors, peripheral T cells are usually either CD4+ or CD8+ with a small percentage of CD4+ CD8+ DP cells (<5%)...
2018: Frontiers in Immunology
Marco Iannetta, Stefano Savinelli, Raffaella Rossi, Claudia Mascia, Raffaella Marocco, Serena Vita, Paola Zuccalà, Maria Antonella Zingaropoli, Fabio Mengoni, Anna Paola Massetti, Mario Falciano, Gabriella d'Ettorre, Maria Rosa Ciardi, Claudio Maria Mastroianni, Vincenzo Vullo, Miriam Lichtner
HIV infection is characterized by a state of chronic activation of the immune system, which is not completely reversed by antiretroviral treatment (ART). The aim of this study was to assess myeloid and lymphoid activation markers during HIV infection, before and one year after ART initiation, in AIDS and non-AIDS presenters. Treatment naïve HIV positive patients were enrolled in this study. Myeloid dendritic cell (mDC), plasmacytoid dendritic cell (pDC), slanDC, monocyte and T-lymphocyte cell counts and activation status, were assessed by flow cytometry in peripheral blood samples...
November 28, 2018: Immunobiology
Ahmad Akbari, Ghasem Mosayebi, Ahmad Reza Samiei, Ali Ghazavi
Evidence from various studies suggests that narcotics abuse may exert adverse immunomodulatory effects on immune responses. The aim of this research was to understand the effects of detoxification with methadone on the percentage of dendritic cells (DCs) and expression of its markers in heroin addicts. In this study, myeloid DCs (CD11c+ ) and plasmacytoid DCs (CD123+ ) were examined in two groups. These groups comprised of 20 healthy volunteers and 20 chronic heroin addicts, before and after detoxification with methadone...
December 3, 2018: International Immunopharmacology
Maria A Streltsova, Sofya A Erokhina, Leonid M Kanevskiy, Dean A Lee, William G Telford, Alexander M Sapozhnikov, Elena I Kovalenko
In this work, we analyzed the phenotype and growth of human NK cell clones obtained by the stimulation of individual NK cells with IL-2 and gene-modified K562 feeder cells expressing membrane-bound IL-21 (K562-mbIL21). We generated clones from NK cells at distinct differentiation and activation stages, determined by CD56, CD57 and HLA-DR expression levels. Less differentiated CD56bright NK cell subsets showed higher cloning efficiency compared with more differentiated CD56dim subsets, especially with the CD57bright subset...
2018: PloS One
Amadou Kone, Bassirou Diarra, Keira Cohen, Seydou Diabate, Bourahima Kone, Mahamane T Diakite, Hawa Diarra, Moumine Sanogo, Antieme C G Togo, Yeya Dit Sadio Sarro, Bocar Baya, Nadie Coulibaly, Ousmane Kodio, Chad J Achenbach, Robert L Murphy, Jane L Holl, Sophia Siddiqui, Seydou Doumbia, William R Bishai, Souleymane Diallo, Mamoudou Maiga
Tuberculosis is caused by Mycobacterium tuberculosis complex (MTBC), however, the distribution and frequency of MTBC lineages and sublineages vary in different parts of the globe. Mycobacterium africanum, a member of MTBC is responsible for a large percentage of TB cases in West Africa, however, it is rarely identified outside of this part of the World. Whether or not differential HLA polymorphism (an important host factor) is contributing to the geographic restriction of M. africanum to West Africa is unknown...
December 4, 2018: HLA
Catia T Perciani, Bashir Farah, Rupert Kaul, Mario A Ostrowski, Salaheddin M Mahmud, Omu Anzala, Walter Jaoko, Kelly S MacDonald
BACKGROUND: Varicella-zoster virus (VZV) is under consideration as a promising recombinant viral vector to deliver foreign antigens including HIV. However, new vectors have come under increased scrutiny since vaccination with Ad5-vectored HIV vaccine trials demonstrated increased HIV risk in individuals with pre-immunity to the vector which was thought to be associated with mucosal immune activation (IA). Therefore, defining the impact of VZV vaccination on IA is particularly important with the prospect of developing an HIV/VZV chimeric vaccine...
December 4, 2018: Journal of Clinical Investigation
Natasa Smrekar, David Drobne, Lojze M Smid, Ivan Ferkolj, Borut Stabuc, Alojz Ihan, Andreja Natasa Kopitar
Background Dendritic cells play crucial roles in the control of inflammation and immune tolerance in the gut. We aimed to investigate the effects of tumor necrosis factor alpha (TNFa) inhibitors on intestinal dendritic cells in patients with inflammatory bowel disease and the potential role of intestinal dendritic cells in predicting the response to treatment. Patients and methods Intestinal biopsies were obtained from 30 patients with inflammatory bowel disease before and after treatment with TNFa inhibitors...
November 26, 2018: Radiology and Oncology
Yunpeng Shi, Chengrui Nan, Zhongjie Yan, Liqiang Liu, Jingjing Zhou, Zongmao Zhao, Depei Li
Objective: The human umbilical cord mesenchymal stem cells (hUMSCs) are characterized with the potential ability to differentiate to several types of cells. Edaravone has been demonstrated to prevent the hUMSCs from the oxidative damage, especially its ability in antioxidative stress. We hypothesized that Edaravone induces the hUMSCs into the neuron-like cells. Methods: The hUMSCs were obtained from the human umbilical cord tissue. The differentiation of hUMSCs was induced by Edaravone with three different doses: 0...
2018: Stem Cells International
Oliver Grauer, Mohammed Jaber, Katharina Hess, Matthias Weckesser, Wolfram Schwindt, Stephan Maring, Johannes Wölfer, Walter Stummer
BACKGROUND: There is an increasing interest in local tumor ablative treatment modalities that induce immunogenic cell death and the generation of antitumor immune responses. METHODS: We report six recurrent glioblastoma patients who were treated with intracavitary thermotherapy after coating the resection cavity wall with superparamagnetic iron oxide nanoparticles ("NanoPaste" technique). Patients underwent six 1-h hyperthermia sessions in an alternating magnetic field and, if possible, received concurrent fractionated radiotherapy at a dose of 39...
December 1, 2018: Journal of Neuro-oncology
Akihiro Yachie
Rapid, precise diagnosis of Epstein-Barr virus-associated T lymphocyte or natural killer cell lymphoproliferative diseases is clinically important to prevent disease progression and avoid fatal outcomes for patients. In addition to detecting increased copy numbers of Epstein-Barr virus, identification of the lymphocyte subpopulation targeted by the virus infection is crucial to reaching the final diagnosis. However, these procedures are laborious and require large amounts of sample. In contrast, flowcytometric analysis may provide crucial information for initial screening of diseases using only small amounts of sample and involves little labor...
2018: Frontiers in Pediatrics
Lucas Pires Garcia Oliveira, Fernanda Lopes Conte, Eliza de Oliveira Cardoso, Bruno José Conti, Karina Basso Santiago, Marjorie de Assis Golim, Geórgia da Silva Feltran, Willian Fernando Zambuzzi, José Maurício Sforcin
AIMS: Chemotherapy has been widely used to treat cancer although it may affect non-target cells involved in the immune response. This work aimed at elucidating whether the chemotherapeutic agent doxorubicin in combination with geopropolis produced by Melipona fasciculata Smith could affect nontumor immune cells, evaluating their immunomodulatory effects on human monocytes. MAIN METHODS: Cell viability, expression of cell markers (HLA-DR, TLR-2, TLR-4, C80 and CD40), cytokine production (TNF-α, IL-1β, IL-6 and IL-10), intracellular pathways (NF-κB and autophagy), the microbicidal activity of monocytes and hydrogen peroxide (H2 O2 ) production were analyzed...
November 29, 2018: Life Sciences
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