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Jingyu Deng, Jinyuan Liu, Wei Wang, Zhe Sun, Zhenning Wang, Zhiwei Zhou, Huimian Xu, Han Liang
Objective: To validate the necessity of increasing the examined lymph node (ELN) count for enhancing the accuracy of prognostic evaluation of gastric cancer (GC) patients after curative gastrectomy in multiple medical centers of China. Methods: The clinicopathological data of 7,620 patients who underwent the curative resection for GC between 2001 and 2011 were included to demonstrate whether the ELN count is indispensable for enhancing the accuracy of prognostic evaluation of GC patients after surgery...
October 2018: Chinese Journal of Cancer Research, Chung-kuo Yen Cheng Yen Chiu
Florian Burger, Michele Angioni, Gianluca Russo, Martina Schad, Jim Kallarackal
BACKGROUND: Primer design is a crucial step in establishing specific and sensitive qPCR assays. Even though numerous tools for primer design exist, the majority of resulting assays still requires extensive testing and optimisation or does not allow for high quality target amplification. We developed a workflow for designing qPCR assays. Unlike other tools, we compute a PCR assay including primer design, concentrations and the optimal PCR program. RESULTS: Gene expression assays were already generated in a total of 283,226 genes from three species and are continued for all genes of the major model species...
November 22, 2018: BMC Bioinformatics
Yun Teng, Yi Ren, Mohammed Sayed, Xin Hu, Chao Lei, Anil Kumar, Elizabeth Hutchins, Jingyao Mu, Zhongbin Deng, Chao Luo, Kumaran Sundaram, Mukesh K Sriwastva, Lifeng Zhang, Michael Hsieh, Rebecca Reiman, Bodduluri Haribabu, Jun Yan, Venkatakrishna Rao Jala, Donald M Miller, Kendall Van Keuren-Jensen, Michael L Merchant, Craig J McClain, Juw Won Park, Nejat K Egilmez, Huang-Ge Zhang
The gut microbiota can be altered by dietary interventions to prevent and treat various diseases. However, the mechanisms by which food products modulate commensals remain largely unknown. We demonstrate that plant-derived exosome-like nanoparticles (ELNs) are taken up by the gut microbiota and contain RNAs that alter microbiome composition and host physiology. Ginger ELNs (GELNs) are preferentially taken up by Lactobacillaceae in a GELN lipid-dependent manner and contain microRNAs that target various genes in Lactobacillus rhamnosus (LGG)...
November 14, 2018: Cell Host & Microbe
Tatiana Dorfman, Mariya Neymark, Julia Begal, Yoram Kluger
BACKGROUND: Enlarged lymph nodes (ELN) pose a great diagnostic challenge. They may represent the first clinical finding of a hematologic disease or other malignancy and may be an indication of a wide range of infectious and non-infectious diseases. Because many patients undergo percutaneous biopsy, surgical excisional biopsy is not often considered. OBJECTIVES: To analyze indications for a patient's referral for surgical biopsy of ELN and diagnostic steps to follow until referral, and to determine the number of ELN...
November 2018: Israel Medical Association Journal: IMAJ
Alexandra Gomez-Arteaga, Monica L Guzman
Monitoring measurable (minimal) residual disease (MRD) in acute myeloid leukemia (AML) has greatly increased our ability to assess chemosensisitivity to treatment as well as the duration of treatment responses. There is strong evidence to support its prognostic value for long-term outcomes at different time points and across assays and targets. It's role as a surrogate endpoint to define risk-adapted strategies is still under evaluation. In this chapter, we will discuss the definition of MRD in AML, the potential contribution of leukemia stem cells (LSCs) to MRD and we will review all the current approaches to assess residual disease including the 2018 European Leukemia Network (ELN) working group recommendations for MRD standardization in AML...
2018: Advances in Experimental Medicine and Biology
Masahiro Sakaguchi, Hiroki Yamaguchi, Yuho Najima, Kensuke Usuki, Toshimitsu Ueki, Iekuni Oh, Sinichiro Mori, Eri Kawata, Nobuhiko Uoshima, Yutaka Kobayashi, Shinichi Kako, Kenji Tajika, Seiji Gomi, Katsuhiro Shono, Kensuke Kayamori, Masao Hagihara, Junya Kanda, Hitoji Uchiyama, Junya Kuroda, Naoyuki Uchida, Yasushi Kubota, Shinya Kimura, Saiko Kurosawa, Nana Nakajima, Atsushi Marumo, Ikuko Omori, Yusuke Fujiwara, Shunsuke Yui, Satoshi Wakita, Kunihito Arai, Tomoaki Kitano, Kazuhiko Kakihana, Yoshinobu Kanda, Kazuteru Ohashi, Takahiro Fukuda, Koiti Inokuchi
In the opinion of the European LeukemiaNet (ELN), nucleophosmin member 1 gene mutation ( NPM1 mut)-positive acute myeloid leukemia (AML) with an fms-like kinase 3-internal tandem duplication ( FLT3 -ITD) allele ratio (AR) <0.5 (low AR) has a favorable prognosis, and allogeneic hematopoietic stem cell transplant (allo-HSCT) in the first complete remission (CR1) period is not actively recommended. We studied 147 patients with FLT3 -ITD gene mutation-positive AML, dividing them into those with low AR and those with AR of ≥0...
October 23, 2018: Blood Advances
Marius Catalin Staiculescu, Austin Cocciolone, Jesse Procknow, Jungsil Kim, Jessica E Wagenseil
Elastic fibers provide reversible elasticity to the large arteries and are assembled during development when hemodynamic forces are increasing. Mutations in elastic fiber genes are associated with cardiovascular disease. Mice lacking expression of the elastic fiber genes elastin (Eln-/- ), fibulin-4 (Efemp2-/- ), or lysyl oxidase (Lox-/- ) die at birth with severe cardiovascular malformations. All three genetic knockout models have elastic fiber defects, aortic wall thickening, and arterial tortuosity. However, Eln-/- mice develop arterial stenoses, while Efemp2-/- and Lox-/- mice develop ascending aortic aneurysms...
October 12, 2018: Physiological Genomics
Laura Jamrog, Guillaume Chemin, Vincent Fregona, Lucie Coster, Marlène Pasquet, Chloé Oudinet, Nelly Rouquié, Naïs Prade, Stéphanie Lagarde, Charlotte Cresson, Sylvie Hébrard, Ngoc Sa Nguyen Huu, Marina Bousquet, Cathy Quelen, Pierre Brousset, Stéphane J C Mancini, Eric Delabesse, Ahmed Amine Khamlichi, Bastien Gerby, Cyril Broccardo
PAX5 is a well-known haploinsufficient tumor suppressor gene in human B-cell precursor acute lymphoblastic leukemia (B-ALL) and is involved in various chromosomal translocations that fuse a part of PAX5 with other partners. However, the role of PAX5 fusion proteins in B-ALL initiation and transformation is ill-known. We previously reported a new recurrent t(7;9)(q11;p13) chromosomal translocation in human B-ALL that juxtaposed PAX5 to the coding sequence of elastin ( ELN ). To study the function of the resulting PAX5-ELN fusion protein in B-ALL development, we generated a knockin mouse model in which the PAX5-ELN transgene is expressed specifically in B cells...
October 9, 2018: Proceedings of the National Academy of Sciences of the United States of America
Manohar Rebecca, Ramakrishnan Gayathri, Renganathan Bhuvanasundar, Krishnamoorthy Sripriya, Balekudaru Shantha, Narayanasamy Angayarkanni
BACKGROUND: Pseudoexfoliation syndrome (PXF) is an idiopathic, elastogenesis-associated systemic disease characterised by amyloid-like material aggregates in the eye. Elevated plasma and aqueous humour (aqH) homocysteine (Hcy) is reportedly associated with PXF. This study is aimed to probe Hcy-mediated alterations in elastin expression. METHODOLOGY: Lens level of Hcy (total Hcy (tHcy)), mRNA expression of Eln , CBS and MTR in lens capsule , protein expression of elastin in aqH were estimated by enzyme immunoassay, quantitative PCR and western blot, respectively in PXF, PXF with glaucoma (PXF-G) cases, in comparison with cataract-alone disease controls...
September 24, 2018: British Journal of Ophthalmology
Motofumi Saitoh, Xiang Gao, Takafumi Ogawa, Yumi H Ikuhara, Shunsuke Kobayashi, Craig A J Fisher, Akihide Kuwabara, Yuichi Ikuhara
Electrical conductivity, state of charge and chemical stability of Li-ion battery materials all depend on the electronic states of their component atoms, and tools for measuring these reliably are needed for advanced materials analysis and design. Here we report a systematic investigation of electron energy-loss near-edge structures (ELNES) of Li-K and O-K edges for ten representative Li-ion battery electrodes and solid-state electrolytes obtained by performing transmission electron microscopy with a Wien-filter monochromator-equipped microscope...
October 3, 2018: Physical Chemistry Chemical Physics: PCCP
Satoshi Hayano, Yusuke Okuno, Makiko Tsutsumi, Hidehito Inagaki, Yoshie Fukasawa, Hiroki Kurahashi, Seiji Kojima, Yoshiyuki Takahashi, Taichi Kato
BACKGROUND: Supravalvular aortic stenosis (SVAS) is a congenital heart disease affecting approximately 1:25,000 live births. SVAS may occur sporadically, be inherited in an autosomal dominant manner, or be associated with Williams-Beuren syndrome, a complex developmental disorder caused by a microdeletion of chromosome 7q11.23. ELN on 7q11.23, which encodes elastin, is the only known gene to be recurrently mutated in less than half of SVAS patients. METHODS: Whole-exome sequencing (WES) was performed for seven familial SVAS families to identify other causative gene mutations of SVAS...
September 13, 2018: International Journal of Cardiology
Ze-Long Yang, Ming-Hua Zhu, Quan Shi, Fu-Min Lu, Chun-Xi Wang
BACKGROUND: Our aim was to evaluate the prognostic value of the number of lymph nodes examined (eLNs) in patients with node-negative gastric cancer (GC) and further to adjust the American Joint Committee on Cancer (AJCC) 8th staging system based on the number of eLNs. METHODS: Node-negative GC patients diagnosed during 1988-2015 from the Surveillance, Epidemiology, and End Results (SEER) database were included. On the basis of a primary cohort of 4159 node-negative GC patients, we built the adjusted AJCC 8th staging system, which was then internally validated by a bootstrap method, and externally validated with an independent cohort of 5565 node-negative GC patients...
September 17, 2018: Journal of Gastrointestinal Surgery: Official Journal of the Society for Surgery of the Alimentary Tract
Seo-Yeon Park, Eun Jung Byun, Jeong Deuk Lee, Sungjoo Kim, Hei Sung Kim
A World Health Organization (WHO) report from 2016 states that over 3 million people die annually from air pollution, which places air pollution as the world's largest single environmental health risk factor. Particulate matter (PM) is one of the main components of air pollution, and there is increasing evidence that PM exposure exerts negative effects on the human skin. To see the impact of air pollution on skin aging, we analyzed the effect of PM exposure on human dermal fibroblasts (HDFs) with Western blot, enzyme-linked immunosorbent assay (ELISA), and gene analysis...
September 12, 2018: International Journal of Molecular Sciences
Karsten Spiekermann, Anne-Sophie Shen
There are several changes in the revised WHO classification for acute leukemia. The latest version of the European Leukemia Network (ELN)-risk classification defines AML with mutations in RUNX1, ASXL1 or TP53 to fall in the unfavorable risk group. Consequently, the somatic molecular genetic testing at the time of initial diagnosis should encompass these before mentioned three genes next to the already routine testing of NPM1, CEBPA and FLT3-ITD. Several new innovative substances have been developed and approved for AML therapy...
September 2018: Deutsche Medizinische Wochenschrift
Shin Kiyohara, Tomohiro Miyata, Koji Tsuda, Teruyasu Mizoguchi
Spectroscopy is indispensable for determining atomic configurations, chemical bondings, and vibrational behaviours, which are crucial information for materials development. Despite their importance, the interpretation of spectra using "human-driven" methods, such as the manual comparison of experimental spectra with reference/simulated spectra, is difficult due to the explosive increase in the number of experimental spectra to be observed. To overcome the limitations of the "human-driven" approach, we develop a new "data-driven" approach based on machine learning techniques by combining the layer clustering and decision tree methods...
September 6, 2018: Scientific Reports
Tarcila Santos Datoguia, Elvira Deolinda Rodrigues Pereira Velloso, Ricardo Helman, Juliane Garcez Musacchio, Marco Aurélio Salvino, Rodolfo Almeida Soares, Marcia Higashi, Adriana Valente Fadel, Rodrigo Santucci Alves E Silva, Nelson Hamerschlak, Fabio Pires de Souza Santos, Paulo Vidal Campregher
Prognostic stratification in acute myeloid leukemia (AML) relies, mostly, on cytogenetics and molecular features of leukemic blasts. The LeukemiaNet prognostic scoring system has been proposed as a standardized way of evaluating prognosis in AML. We have analysed outcomes in 65 AML cases (median age of 54 years, range 18-82) treated at five hematology centers in Brazil stritified according to the European Leukemia Net (ELN) recommendations for cytogenetic and molecular analysis. We classified patients as favorable (N = 13), intermediate-1 (N = 25), intermediate-2 (N = 15), or adverse risk (N = 9)...
September 5, 2018: Medical Oncology
Seung-Taek Lim, Jin-Kee Park, Sang-Hyuk Park, Eun-Jae Lee, Woo-Nam Kim, Seok-Ki Min
PURPOSE: Elastin is one of the major determinants of arterial distensibility of large blood vessels that forms the principal component of elastic fibers from the media of arteries. However, the association between elastin(ELN) genotype and vascular function is still unclear. METHODS: 120women were recruited from the Saha-gu (Busan, Korea) Community Center. Measurements of body composition and vascular function included carotid intima-media thickness (CIMT), carotid artery luminal diameter (CLD), minimum (diastolic) artery luminal diameter (CLDmin) and maximum (systolic) artery luminal diameter (CLDmax)...
June 30, 2018: Journal of Exercise Nutrition & Biochemistry
R Jorritsma, I M van Geijlswijk, M Nielen
Societal concerns about antibiotic resistance prompted us to evaluate the use of prophylactic and postoperative antibiotic treatments following cesarean section (CS) and exploratory laparotomy (EL) in a surgery theater within the Farm Animal Health clinic. All procedures were performed by supervised veterinary students for training purposes. Cows undergoing CS (n = 100) received either a prophylaxis with a single 5-g injection of ampicillin-sodium i.v. only (CSL), or in combination with postoperative i.m. injections of ampicillin-trihydrate 20% for 3 d (10 mg/kg, twice a day; CSH)...
September 2018: Journal of Dairy Science
Kazuyuki Yo, Thomas M Rünger
Several features differentiate aged cells from young cells, many of which are due to changes in gene expression during the aging process. The mechanisms of altered gene expression in aging cells remain incompletely understood, and we hypothesized that long non-coding (lnc) RNAs mediate at least some of these changes. We screened for alterations in lncRNA expression with aging in skin fibroblasts and identified the lncRNA FLJ46906 to be consistently upregulated with aging in-vivo and in-vitro. The function of this lncRNA has not been known...
August 17, 2018: Aging
Armin Kraus, Daniel Sattler, Markus Wehland, Ronald Luetzenberg, Nauras Abuagela, Manfred Infanger
BACKGROUND: In obtaining human tenocytes in a sufficient number for tendon tissue engineering, low proliferation rate and phentoype loss during passaging is a problem. It was our aim to evalute the influence of VEGF on human tenocyte growth and gene expression. MATERIALS AND METHODS: Human tenocytes were obtained from surgical specimens and exposed to human VEGF in various concentrations (5ng/ml, 10ng/ml, 20ng/ml) for 5 days. Cell proliferation was counted and expression of tendon-related genes (Col1, Col3, TSC, SCX and ELN) was analyzed...
August 11, 2018: Plastic and Reconstructive Surgery
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