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citrullinated protein antibody

Ko-Ming Lin, Wei-Ming Chen, Shui-Yi Tung, Kuo-Liang Wei, Chein-Heng Shen, Te-Sheng Chang, Pey-Jium Chang
AIM: In order to increase diagnostic sensitivity for early disease in rheumatoid arthritis (RA), new classification criteria were approved in 2010 by the American College of Rheumatology and the European League Against Rheumatism. One of the criteria, a high-positive rheumatoid factor (RF) or anti-citrullinated protein antibody (ACPA) level, was given a high score of 3. However, the increased prevalence of RF in patients with chronic hepatitis C virus (HCV) infection markedly diminishes the diagnostic specificity of serum RF for RA in these patients...
October 18, 2018: International Journal of Rheumatic Diseases
Viviane Malmström, Johanna Steen, Lars Klareskog
Rikard Holmdahl has commented on our recently published paper on specificity and function of human monoclonal antibodies (mAbs) from synovial plasma cells that react with citrullinated proteins and peptides (1). He also comments on our previous study from 2013 (2) that was based on mAbs generated from synovial B cells. Dr Holmdahl's letter provides us with an opportunity to discuss and clarify some methodological issues concerning RA monoclonal autoantibody expression, specificity, and functionality. In our eyes, cloning and characterization of human monoclonal autoantibodies from patients represents an important approach to the understanding of origins and functions of autoantibodies in the pathogenesis of immune-mediated diseases...
October 10, 2018: Arthritis & Rheumatology
Lianne P W M Lelieveldt, Hendy Kristyanto, Ger J M Pruijn, Hans Ullrich Scherer, Rene E M Toes, Kimberly M Bonger
Autoreactive B cells are thought to play a pivotal role in many autoimmune diseases. Rheumatoid arthritis (RA) is an autoimmune disease affecting ~1% of the Western population and is hallmarked by the presence of anti-citrullinated proteins antibodies (ACPA) produced by autoreactive B cells. We intend to develop a method to target and selectively eliminate these autoreactive B cells using a sequential antigen prodrug targeting strategy. As ACPA-expressing B cells are thought to play essential roles in RA-disease pathogenesis, we used this B-cell response as prototype to analyze the feasibility to generate a construct consisting of a biologically silenced, i...
October 5, 2018: Molecular Pharmaceutics
Hoshimi Kawaguchi, Isao Matsumoto, Atsumu Osada, Izumi Kurata, Hiroshi Ebe, Yuki Tanaka, Asuka Inoue, Naoto Umeda, Yuya Kondo, Hiroto Tsuboi, Akihito Ishigami, Takayuki Sumida
OBJECTIVE: To explore the relevance of citrullinated proteins and anti-citrullinated protein antibodies (ACPA) via protein arginine deiminase (PAD) inhibition in peptide glucose-6-phosphate isomerase-induced arthritis (pGIA). METHODS: Cl-amidine, a PAD inhibitor, was injected into pGIA. Clinical scores and histopathological findings of ankle joints were assessed. Serum ACPA titers were analyzed using ELISA. Citrullinated protein expression in joints and sera were examined with immunohistochemistry and Western blot analysis, respectively...
October 4, 2018: Modern Rheumatology
Mônica G Corrêa, Paula Rodrigues Pires, Fernanda Vieira Ribeiro, Suzana Peres Pimentel, Fabiano Ribeiro Cirano, Marcelo Henrique Napimoga, Marcio Z Casati, Renato Corrêa Viana Casarin
Rheumatoid arthritis and periodontitis are chronic inflammatory diseases which has been closely associated due to the nature of immune-inflammatory imbalance response. Resveratrol is a naturall product with biological proprieties that may promote immunomodulatory effects on host response. This study investigated resveratrol continuous administration effect on experimental periodontitis and arthritis progression in rats. Thirty-five rats were assigned to the following groups: 1-experimental arthritis + experimental periodontitis + placebo (RA+EP +PL) (n = 12); 2 -RA+EP+ ibuprofen (RA+PE+IB) (n = 11); 3-RA+EP+ resveratrol (RA+PE+RSV) (n = 11)...
2018: PloS One
Kristine Germar, Cynthia M Fehres, Hans U Scherer, Nathalie van Uden, Sabrina Pollastro, Nataliya Yeremenko, Monika Hansson, Priscilla F Kerkman, Ellen I H van der Voort, Evan Reed, Hanna Maassen, Mark J Kwakkenbos, Arjen Q Bakker, Lars Klareskog, Vivianne Malmström, Niek de Vries, René E M Toes, Karin Lundberg, Hergen Spits, Dominique L Baeten
OBJECTIVE: Anti-citrullinated protein antibodies (ACPA) are a hallmark for rheumatoid arthritis (RA). Autoantigen-specific B-cell function aside from autoantibody production remains poorly understood in the context of this disease. We set out to elucidate this function through the isolation and immortalization of unique citrullinated protein or peptide (CP)-reactive B-cell clones from RA patients. METHODS: B-cell clones from either blood or synovial fluid of cyclic-citrullinated peptide 2 (CCP2)+ RA patients were immortalized by genetic reprogramming with Bcl6 and Bcl-xL...
October 1, 2018: Arthritis & Rheumatology
N Molano-González, E Olivares-Martínez, J M Anaya, G Hernández-Molina
OBJECTIVE: The presence of anti-citrullinated protein antibodies (ACPAs) in primary Sjögren's syndrome (pSS) ranges from 3% to 9.9%; however, there is no agreement about their clinical significance. Our aim was to systematically review the literature regarding the association of arthritis and ACPAs in pSS and their role in the development of rheumatoid arthritis (RA). METHOD: A comprehensive search of MEDLINE, ISI Web of Knowledge, and Cochrane Library from inception until June 2016 was undertaken using the combination of two or three of the keywords: primary Sjögren's syndrome, Sjögren's syndrome, arthritis, synovitis, arthropathy, anti-cyclic citrullinated peptide antibodies, and anti-citrullinated protein antibody - ACPA...
September 30, 2018: Scandinavian Journal of Rheumatology
K Chatzidionysiou, G Lukina, C Gabay, M L Hetland, E M Hauge, K Pavelka, D Nordström, H Canhão, M Tomsic, Z Rotar, E Lie, T K Kvien, R F van Vollenhoven, S Saevarsdottir
OBJECTIVES: To investigate whether smoking habits predict response to rituximab (RTX) in rheumatoid arthritis (RA). METHOD: We included patients from the CERERRA international cohort receiving the first treatment cycle with available smoking status (n = 2481, smokers n = 528, non-current smokers n = 1953) and at least one follow-up visit. Outcome measures were change in Disease Activity Score based on 28-joint count (ΔDAS28) and European League Against Rheumatism (EULAR) good response at 6 months, with non-current smokers as the referent group...
September 27, 2018: Scandinavian Journal of Rheumatology
Meghdad Abdollahpour-Alitappeh, Majid Lotfinia, Nader Bagheri, Koushan Sineh Sepehr, Mahdi Habibi-Anbouhi, Farzad Kobarfard, Saeed Balalaie, Alireza Foroumadi, Ghasem Abbaszadeh-Goudarzi, Kazem Abbaszadeh-Goudarzi, Mohsen Abolhassani
Targeted therapy using specific monoclonal antibodies (mAbs) conjugated to chemotherapeutic agents or toxins has become one of the top priorities in cancer therapy. Antibody-drug conjugates (ADCs) are emerging as a promising strategy for cancer-targeted therapy. In this study, trastuzumab, a humanized monoclonal anti-HER2 antibody, was reduced by dithiothreitol and conjugated to the microtubule-disrupting agent monomethyl auristatin E (MMAE) through a valine-citrulline peptide linker (trastuzumab-MC-Val-Cit-PABC-MMAE [trastuzumab-vcMMAE])...
September 24, 2018: Journal of Cellular Physiology
Amir Taherkhani, Shiva Kalantari, Afsaneh Arefi Oskouie, Mohsen Nafar, Mohammad Taghizadeh, Koorosh Tabar
Membranous glomerulonephritis (MGN) is one of the most frequent causes of nephrotic syndrome in adults. It is characterized by the thickening of the glomerular basement membrane in the renal tissue. The current diagnosis of MGN is based on renal biopsy and the detection of antibodies to the few podocyte antigens. Due to the limitations of the current diagnostic methods, including invasiveness and the lack of sensitivity of the current biomarkers, there is a requirement to identify more applicable biomarkers...
September 12, 2018: Molecular Medicine Reports
Qing Luo, Pengcheng Xiao, Xue Li, Zhen Deng, Cheng Qing, Rigu Su, Jianqing Xu, Yang Guo, Zikun Huang, Junming Li
It is well-known that monocytes are a heterogeneous cell population and different monocyte subsets play important roles in rheumatoid arthritis (RA). Cluster of differentiation (CD)64 is one of Fc receptor, which initiates immunological and inflammatory reactions. However, the roles in RA remain to be elucidated. In the present study, the expression of CD64, CD40, CD163, CD206, HLA-DR, CD80 and CD86 on monocytes and the expression of CD64 on monocyte subsets were determined by flow cytometry. The expression of CD64 on monocyte subsets in patients with RA was further analyzed for their correlation with markers of autoimmune response, inflammation, disease activity of RA and serum cytokines...
September 2018: Experimental and Therapeutic Medicine
Sigita Stropuvienė, Asta Baranauskaitė, Loreta Bukauskienė, Jolanta Zaikauskienė
Background: To provide data on the use of anti-cyclic citrullinated peptide antibody (anti-CCP) and other routinely used clinical parameters and to assess the impact of anti-CCP status on therapeutic decisions, an observational study was conducted in patients with rheumatoid arthritis (RA). Methods: Sixty-seven adult patients with a recent diagnosis of RA were recruited from four rheumatology centres in Lithuania and were prospectively observed for 12 months. Data collection was based on the review of medical records and routine examination of patients...
2018: Acta Medica Lituanica
Y Du, J W Wang, M J Zhou, X Y Wu, J Xue, H X Wu
Objective: To investigate the clinical significance of soluble Semaphorin 5A(Sema 5A) in patients of rheumatoid arthritis(RA) and the effect of Sema 5A on osteoclastogenesis in RAW264.7 cells. Methods: (1)Soluble Sema 5A was detected in the serum of 62 RA patients, 30 osteoarthritis(OA) patients and 48 healthy controls(HC) by enzyme-linked immunosorbent assay(ELISA).The relationships between serum Sema 5A and disease activity, radiographic severity and laboratory parameters were investigated in RA patients...
September 1, 2018: Zhonghua Nei Ke za Zhi [Chinese Journal of Internal Medicine]
Katarzyna Kolarz, Bożena Targońska-Stępniak, Maria Majdan
Rheumatoid arthritis (RA) is a systemic, autoimmune disease characterized by synovitis and irreversible joint destruction. RA development is a multi-stage process conditioned by the presence of genetic and environmental risk factors on the basis of which systemic immunization develops. The best known genetic factor predisposing to RA development is the polymorphism of HLA-DRB1 region of the major histocompatibility complex. Among environmental factors, importance in the pathogenesis of RA is attributed to the development of inflammation of the respiratory tract and chronic periodontitis caused by Porphyromonas gingivalis...
2018: Wiadomości Lekarskie: Organ Polskiego Towarzystwa Lekarskiego
Hitomi Kobayashi, Yasuyuki Kobayashi, Isamu Yokoe, Noboru Kitamura, Atsuma Nishiwaki, Masami Takei, Jon T Giles
OBJECTIVE: Individuals with rheumatoid arthritis (RA) are at a heightened risk of sudden cardiac death, an outcome increased in those with prolongation of the corrected electrocardiographic QT interval (QTc). We compared QTc between patients with RA and demographically matched controls and studied the change in QTc after treatment with the interleukin 6 inhibitor tocilizumab (TCZ). METHODS: Standard 12-lead electrocardiograms were obtained and QTc was measured in patients with RA at baseline and after 24 weeks of TCZ treatment, then compared with non-RA controls who were frequency-matched on age and sex...
September 1, 2018: Journal of Rheumatology
Hussein Kadhem Al-Hakeim, Shatha Rouf Moustafa, Khlowd Mohammed Jasem
A considerable attention has been focused on the possible association between ultra-trace elements (UTEs) status and pathogenesis of many diseases including rheumatoid arthritis (RA). UTEs have important roles in numerous metabolic processes. Serum Cs, Rb, and Re levels in RA are not studied previously. The correlation of serum Cs, Rb, and Re levels with the well-known serological parameters, anticyclic citrullinated protein antibody (ACPA), C-reactive protein (CRP), ESR, and rheumatoid factor (RF) is also not studied previously...
August 31, 2018: Biological Trace Element Research
Eun Seong Park, Sung Soo Ahn, Seung Min Jung, Jungsik Song, Yong-Beom Park, Sang-Won Lee
AIM: To apply 2012 European League Against Rheumatism/American College of Rheumatology (EULAR/ACR) criteria to Korean patients previously classified as polymyalgia rheumatica (PMR) by Chuang and Hunder criteria or Healey criteria and investigated whether they might be still reclassified as PMR or not. METHODS: We retrospectively reviewed the medical records of 113 previously classified PMR patients. We applied 2012 EULAR/ACR criteria without ultrasonography to PMR patients, and fulfilment required at least 4 points...
August 30, 2018: International Journal of Rheumatic Diseases
Dres Damgaard, Mandar Bawadekar, Ladislav Senolt, Allan Stensballe, Miriam A Shelef, Claus H Nielsen
OBJECTIVE: Peptidylarginine deiminase 2 (PAD2) and PAD4 are expressed in the synovium of rheumatoid arthritis (RA) patients and catalyze citrullination of arginine residues in proteins targeted by anti-citrullinated protein antibodies (ACPAs). Little is known about the relative importance of PAD2 and PAD4 in generating citrullinated self-antigens. Here we investigate the ability of PAD2 and PAD4 to generate citrullinated targets for ACPAs in four human proteins. METHODS: Synovial fluid (SF) and plasma were collected from 42 RA patients...
2018: PloS One
Johanna Steen, Björn Forsström, Peter Sahlström, Victoria Odowd, Lena Israelsson, Akilan Krishnamurthy, Sara Badreh, Linda Mathsson Alm, Joanne Compson, Daniel Ramsköld, Welcome Ndlovu, Stephen Rapecki, Monika Hansson, Philip J Titcombe, Holger Bang, Daniel L Mueller, Anca I Catrina, Caroline Grönwall, Karl Skriner, Peter Nilsson, Daniel Lightwood, Lars Klareskog, Vivianne Malmström
OBJECTIVES: Antibodies against post-translationally modified (PTM) proteins are a hallmark of rheumatoid arthritis (RA), but the emergence and pathogenicity of these autoantibodies are still incompletely understood. We aimed to analyze the antigen specificities and mutation patterns of monoclonal antibodies derived from RA synovial plasma cells and address the question of antigen cross reactivity. METHODS: IgG secreting cells were isolated from RA synovial fluid, and the variable regions of the immunoglobulins were sequenced (n=182) and expressed in full-length antibodies (n=93) and also as germline reverted versions...
August 27, 2018: Arthritis & Rheumatology
Changrong Ge, Bingze Xu, Bibo Liang, Erik Lönnblom, Susanna L Lundström, Roman A Zubarev, Burcu Ayoglu, Peter Nilsson, Thomas Skogh, Alf Kastbom, Vivianne Malmström, Lars Klareskog, René E M Toes, Theo Rispens, Doreen Dobritzsch, Rikard Holmdahl
OBJECTIVES: Anti-citrullinated protein antibodies (ACPAs) develop many years before the clinical onset of rheumatoid arthritis. Here we addressed the molecular basis of the specificity and cross-reactivity of ACPAs from patients with rheumatoid arthritis (RA). METHODS: Antibodies isolated from RA patients were expressed as monoclonal chimeric antibodies with mouse Fc and characterized for glycosylation using mass-spectrometry and cross-reactivity using Biacore and Luminex immunoassay...
August 27, 2018: Arthritis & Rheumatology
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