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citrullinated protein antibody

Erika Darrah, Jon T Giles, Ryan L Davis, Pooja Naik, Hong Wang, Maximilian F Konig, Laura C Cappelli, Clifton O Bingham, Sonye K Danoff, Felipe Andrade
Objective: Peptidylarginine deiminases (PAD) 2 and 4 are key enzymes in rheumatoid arthritis (RA) pathogenesis due to their ability to generate the protein targets of anti-citrullinated protein antibodies (ACPA). Anti-PAD4 antibodies that cross-react with PAD3 (anti-PAD3/4) have been identified and are associated with severe joint and lung disease. Here, we examined whether anti-PAD2 antibodies were present in patients with RA and defined their clinical significance. Patients and Methods: A PAD2 ELISA was established to screen for anti-PAD2 IgG in sera from RA patients from a prospective observational cohort study ( n = 184) and healthy controls ( n = 100)...
2018: Frontiers in Immunology
Fulvia Ceccarelli, Marco Sciandrone, Carlo Perricone, Giulio Galvan, Enrica Cipriano, Alessandro Galligari, Tommaso Levato, Tania Colasanti, Laura Massaro, Francesco Natalucci, Francesca Romana Spinelli, Cristiano Alessandri, Guido Valesini, Fabrizio Conti
OBJECTIVE: Limited evidences are available on biomarkers to recognize Systemic Lupus erythematosus (SLE) patients at risk to develop erosive arthritis. Anti-citrullinated peptide antibodies (ACPA) have been widely investigated and identified in up to 50% of X-ray detected erosive arthritis; conversely, few studies evaluated anti-carbamylated proteins antibodies (anti-CarP). Here, we considered the application of machine learning models to identify relevant factors in the development of ultrasonography (US)-detected erosive damage in a large cohort of SLE patients with joint involvement...
2018: PloS One
Meiju Zhou, Jiawei Wang, Wenjia Sun, Xinyu Wu, Jing Xue, Huaxiang Wu, Yan Du
OBJECTIVE: To investigate the expression of leukocyte immunoglobulin-like receptor A3 (LILRA3) in CD14+ monocytes of patients with rheumatoid arthritis (RA). METHODS: Fifty three RA patients admitted in the Second Affiliated Hospital of Zhejiang University School of Medicine from February 2017 to August 2017, and 21 healthy subjects were enrolled in the study. The expression of LILRA3 in CD14+ monocyte subset was determined by flow cytometry, and its correlations with clinical features, laboratory examination results, antibodies and disease activity were analyzed...
February 25, 2018: Zhejiang da Xue Xue Bao. Yi Xue Ban, Journal of Zhejiang University. Medical Sciences
Erika Darrah, Fang Yu, Laura C Cappelli, Antony Rosen, James R O'Dell, Ted R Mikuls
OBJECTIVE: To determine if the baseline presence of autoantibodies to peptidylarginine deiminase 4 (PAD4) predicts therapeutic response to biologic and conventional disease-modifying antirheumatic drugs (DMARDs) in patients with rheumatoid arthritis (RA) who failed methotrexate (MTX) monotherapy. METHODS: Baseline serum from 282 RA patients who failed MTX monotherapy were screened for the presence of anti-PAD4 antibodies by immunoprecipitation. Clinical response to either triple DMARD (MTX, sulfasalazine, and hydroxychloroquine) or MTX-etanercept combination therapy was determined at 24- and 48-weeks post-treatment initiation...
December 3, 2018: Arthritis & Rheumatology
Hideaki Yamakawa, Eri Hagiwara, Tae Iwasawa, Ryota Otoshi, Erina Tabata, Satoshi Ikeda, Ryo Okuda, Tomohisa Baba, Shinichiro Iso, Koji Okudela, Tamiko Takemura, Shinji Sato, Takashi Ogura
Little has been reported on the characteristics of interstitial lung disease (ILD) associated with anti-citrullinated peptide/protein antibody (ACPA)-positive anti-synthetase syndrome (ASS). We sought to investigate the clinical, radiologic, and pathologic features of patients with ILD associated with ACPA-positive ASS. Medical records of seven ILD patients with positive results of both ACPA and anti-aminoacyl-tRNA synthetase antibody were retrospectively reviewed. Five patients had clinical symptoms associated with ASS other than ILD...
October 2018: Journal of Thoracic Disease
Iman Tarakji, Wafa Habbal, Fawza Monem
Background: STAT4 rs7574865 polymorphism has been evidently associated with susceptibility to Rheumatoid Arthritis (RA) in European and Eastern Asian populations, whereas studies in other countries reported otherwise. Objective: We investigated the distribution of STAT4 rs7574865 polymorphism in a group of Syrian RA patients. Methods: Eighty-one RA patients and forty healthy controls were enrolled and STAT4 rs7574865 was genotyped by direct sequencing...
2018: Open Rheumatology Journal
Vikram Haridas, Kiran Haridas
Aim: To evaluate the prevalence of anti-thyroid antibodies in a cohort of rheumatoid arthritis (RA) patients from south India and their clinical use. Methods: A cross-sectional epidemiological study was conducted including 1217 patients aged more than 14 years with fever and thrombocytopenia admitted in the medical wards from October 2013 to September 2014. Detailed clinical examination and routine investigations were done; specific investigations like blood culture, widal test, antigen test for malaria, IgM ELISA leptospira, IgM ELISA dengue, bone marrow aspiration/biopsy etc...
May 2018: Journal of the Association of Physicians of India
R M Ten Brinck, H W van Steenbergen, A H M van der Helm-van Mil
OBJECTIVE: Subclinical joint inflammation in patients with arthralgia is predictive for progression to rheumatoid arthritis (RA). However, the time course of progression for bone marrow edema (osteitis), synovitis, and/or tenosynovitis is unsettled. This longitudinal study assessed the course of magnetic resonance imaging (MRI)-detected subclinical joint inflammation during progression to RA. METHODS: Patients that progressed from clinically suspect arthralgia (CSA) to RA underwent 1...
November 21, 2018: Arthritis Research & Therapy
Amarshi Mukherjee, Vanessa Jantsch, Rida Khan, Wolfgang Hartung, René Fischer, Jonathan Jantsch, Boris Ehrenstein, Maximilian F Konig, Felipe Andrade
Background: Aggregatibacter actinomycetemcomitans ( Aa ) is a Gram-negative coccobacillus recognized as a pathogen in periodontitis and infective endocarditis. By producing a toxin (leukotoxin A, LtxA) that triggers global hypercitrullination in neutrophils, Aa has been recently linked to rheumatoid arthritis (RA) pathogenesis. Although mechanistic and clinical association studies implicate Aa infection in the initiation of autoimmunity in RA, direct evidence in humans is lacking. Case: We describe a 59-year-old man with anti-citrullinated protein antibody (ACPA)-positive RA who presented for evaluation of refractory disease...
2018: Frontiers in Immunology
Jennifer L Ather, Oliver Dienz, Jonathan E Boyson, Vikas Anathy, Eyal Amiel, Matthew E Poynter
Serum amyloid A (SAA) proteins are a family of acute phase apolipoproteins implicated to directly modulate innate and adaptive immune responses. However, new studies comparing endogenous SAAs and recombinant forms of these proteins have questioned the function of SAA in inflammation and immunity. We generated SAA3 knockout mice to evaluate the contribution of SAA3 to lung development and immune-mediated lung disease. While SAA3 deficiency does not affect the generation of house dust mite-induced allergic asthma, mice lacking SAA3 develop adult-onset obesity, intrinsic airway hyperresponsiveness, increased inflammatory and fibrotic gene expression in the lung, and elevated levels of lung citrullinated proteins...
November 8, 2018: Scientific Reports
Ankit Mahendra, Xingyu Yang, Shaza Abnouf, Jay R T Adolacion, Daechan Park, Sanam Soomro, Jason Roszik, Cristian Coarfa, Gabrielle Romain, Keith Wanzeck, S Louis Bridges, Amita Aggarwal, Peng Qiu, Sandeep K Agarwal, Chandra Mohan, Navin Varadarajan
OBJECTIVE: To obtain the comprehensive transcriptome profile of human citrulline-specific B cells from patients diagnosed with rheumatoid arthritis (RA). METHODS: Citrulline and hemagglutinin (HA) specific B cells were flow-sorted using peptide-streptavidin conjugates from peripheral blood of RA patients and healthy individuals respectively. Transcriptome profile of the sorted cells was obtained by RNA-sequencing, and expression of key protein molecules was evaluated by aptamer-based SOMAscan assay and flow cytometry...
November 8, 2018: Arthritis & Rheumatology
Monika Ponikowska, Jerzy Świerkot, Beata Nowak, Lucyna Korman, Piotr Wiland
OBJECTIVES: The aim of the study was to evaluate the frequency of anti-mutated citrullinated vimentin antibodies (a-Sa), anti-citrullinated α-enolase peptide 1 antibodies (a-CEP-1), anti-filaggrin antibodies (AFAs), heterogeneous nuclear ribonucleoprotein compies/anti-RA33-antibodies (a-hnRNP/RA33), anti-carbamylated protein antibodies (a-CarP), and metalloproteinase (MMPs) activity in patients with early inflammatory arthritis (EIA). METHODS: Seventy-four patients with EIA: 51 diagnosed with RA (rheumatoid arthritis) and 23 with UA (undifferentiated arthritis), and 20 healthy volunteers were enrolled to the study...
November 7, 2018: Clinical Rheumatology
Cliff Rims, Hannes Uchtenhagen, Mariana J Kaplan, Carmelo Carmona-Rivera, Philip Carlucci, Katalin Mikecz, Adrienn Markovics, Jeffrey Carlin, Jane H Buckner, Eddie A James
OBJECTIVE: Recognition of citrullinated antigens such as vimentin, fibrinogen, and alpha-enolase is associated with rheumatoid arthritis (RA). Emerging data suggests that the matrix protein aggrecan is also recognized as a citrullinated antigen. The goal of this study was to directly visualize cit-aggrecan specific T cells and characterize them in subjects with RA. METHODS: Citrullinated aggrecan peptides with likely DRB1*04:01 binding motifs were predicted using a previously published scanning algorithm...
November 2, 2018: Arthritis & Rheumatology
Martin Cornillet, Fruzsina Babos, Anna Magyar, Mireille Sebbag, Evelyne Verrouil, Ferenc Hudecz, Guy Serre, Leonor Nogueira
Quantification of Abs toward a single epitope is critical to understanding immunobiological processes. In autoimmunity, the prognostic value of the serological profiles of patients draws much attention, but the detection of Abs toward a single epitope is not well controlled. Particularly, the rheumatoid arthritis (RA)-specific anti-citrullinated protein/peptide Abs (ACPA) are specific to a two-atom change on arginyl residues and are considered a heterogeneous family of Abs. As a model, we studied ACPA to decipher how peptide features used as immunosorbent impact Ab detection...
December 1, 2018: Journal of Immunology: Official Journal of the American Association of Immunologists
Martijn A H Oude Voshaar, Zofia Das Gupta, Johannes W J Bijlsma, Annelies Boonen, Jeffrey Chau, Delphine S Courvoisier, Jeffrey R Curtis, Benjamin Ellis, Sofia Ernestam, Laure Gossec, Christine Hale, Jennifer Hornjeff, Katy Y Y Leung, Merav Lidar, Phillip Mease, Kaleb Michaud, Girish M Mody, Mwidimi Ndosi, Christina H Opava, Geraldo R C Pinheiro, Matthew Salt, Enrique R Soriano, William J Taylor, Maria J H Voshaar, Angelique E A M Weel, Maarten de Wit, Nico Wulffraat, Mart A F J van de Laar, Harald E Vonkeman
BACKGROUND: The implementation of value based healthcare (VBHC) in inflammatory arthritis (IA) requires a standardized set of modifiable outcomes and risk-adjustment variables that is feasible to implement worldwide. METHODS: The International Consortium for Health Outcomes Measurement (ICHOM) assembled a multidisciplinary working group, consisting of 24 experts from six continents, including six patient representatives, to develop a Standard Set of outcomes for IA...
October 24, 2018: Arthritis Care & Research
Michitaro Hayakawa, Keisuke Izumi, Misako Higashida-Konishi, Mari Ushikubo, Masako Tsukamoto, Kumiko Akiya, Kazuhiro Araki, Hisaji Oshima
Tocilizumab (TCZ) is a humanized antihuman interleukin-6 (IL-6) receptor antibody used for the treatment of inflammatory diseases such as rheumatoid arthritis (RA). While TCZ could act as a therapeutic agent, it has a potential for inducing adverse drug events including psoriasis-like eruption. Seven cases with specific reference to TCZ-induced psoriasis eruption have been reported worldwide so far. In these cases, treatments with the same dosage of TCZ were either maintained or discontinued. Herein, we report a case involving a 74-year-old man diagnosed with rheumatoid factor-positive and anti-citrullinated protein antibody-positive RA with comorbidity of atopic dermatitis...
October 24, 2018: Rheumatology International
Renu Saigal, Surendra Singh Bhakal, Laxmikant Goyal, Akhil D Goel
Objective: To evaluate the prevalence of anti- citrullinated protein antibodies (ACPA) in patients with a variety of rheumatic diseases other than rheumatoid arthritis (RA). Methods: 144 cases of rheumatic diseases other than rheumatoid arthritis (RA) over a period of 1 year were recruited after consenting and followed up for 2 years. Their serum samples were tested for ACPA. Results: ACPA seropositivity of 9.03% was observed in rheumatic diseases other than RA...
April 2018: Journal of the Association of Physicians of India
Ko-Ming Lin, Wei-Ming Chen, Shui-Yi Tung, Kuo-Liang Wei, Chein-Heng Shen, Te-Sheng Chang, Pey-Jium Chang
AIM: In order to increase diagnostic sensitivity for early disease in rheumatoid arthritis (RA), new classification criteria were approved in 2010 by the American College of Rheumatology and the European League Against Rheumatism. One of the criteria, a high-positive rheumatoid factor (RF) or anti-citrullinated protein antibody (ACPA) level, was given a high score of 3. However, the increased prevalence of RF in patients with chronic hepatitis C virus (HCV) infection markedly diminishes the diagnostic specificity of serum RF for RA in these patients...
October 18, 2018: International Journal of Rheumatic Diseases
Viviane Malmström, Johanna Steen, Lars Klareskog
Rikard Holmdahl has commented on our recently published paper on specificity and function of human monoclonal antibodies (mAbs) from synovial plasma cells that react with citrullinated proteins and peptides (1). He also comments on our previous study from 2013 (2) that was based on mAbs generated from synovial B cells. Dr Holmdahl's letter provides us with an opportunity to discuss and clarify some methodological issues concerning RA monoclonal autoantibody expression, specificity, and functionality. In our eyes, cloning and characterization of human monoclonal autoantibodies from patients represents an important approach to the understanding of origins and functions of autoantibodies in the pathogenesis of immune-mediated diseases...
October 10, 2018: Arthritis & Rheumatology
Lianne P W M Lelieveldt, Hendy Kristyanto, Ger J M Pruijn, Hans Ulrich Scherer, René E M Toes, Kimberly M Bonger
Autoreactive B cells are thought to play a pivotal role in many autoimmune diseases. Rheumatoid arthritis (RA) is an autoimmune disease affecting ∼1% of the Western population and is hallmarked by the presence of anticitrullinated proteins antibodies (ACPA) produced by autoreactive B cells. We intend to develop a method to target and selectively eliminate these autoreactive B cells using a sequential antigen prodrug targeting strategy. As ACPA-expressing B cells are thought to play essential roles in RA-disease pathogenesis, we used this B cell response as a prototype to analyze the feasibility to generate a construct consisting of a biologically silenced, that is, blocked, antigen connected to a cytotoxic prodrug...
October 26, 2018: Molecular Pharmaceutics
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