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citrullinated peptide antibody

Fulvia Ceccarelli, Marco Sciandrone, Carlo Perricone, Giulio Galvan, Enrica Cipriano, Alessandro Galligari, Tommaso Levato, Tania Colasanti, Laura Massaro, Francesco Natalucci, Francesca Romana Spinelli, Cristiano Alessandri, Guido Valesini, Fabrizio Conti
OBJECTIVE: Limited evidences are available on biomarkers to recognize Systemic Lupus erythematosus (SLE) patients at risk to develop erosive arthritis. Anti-citrullinated peptide antibodies (ACPA) have been widely investigated and identified in up to 50% of X-ray detected erosive arthritis; conversely, few studies evaluated anti-carbamylated proteins antibodies (anti-CarP). Here, we considered the application of machine learning models to identify relevant factors in the development of ultrasonography (US)-detected erosive damage in a large cohort of SLE patients with joint involvement...
2018: PloS One
Meiju Zhou, Jiawei Wang, Wenjia Sun, Xinyu Wu, Jing Xue, Huaxiang Wu, Yan Du
OBJECTIVE: To investigate the expression of leukocyte immunoglobulin-like receptor A3 (LILRA3) in CD14+ monocytes of patients with rheumatoid arthritis (RA). METHODS: Fifty three RA patients admitted in the Second Affiliated Hospital of Zhejiang University School of Medicine from February 2017 to August 2017, and 21 healthy subjects were enrolled in the study. The expression of LILRA3 in CD14+ monocyte subset was determined by flow cytometry, and its correlations with clinical features, laboratory examination results, antibodies and disease activity were analyzed...
February 25, 2018: Zhejiang da Xue Xue Bao. Yi Xue Ban, Journal of Zhejiang University. Medical Sciences
Hideaki Yamakawa, Eri Hagiwara, Tae Iwasawa, Ryota Otoshi, Erina Tabata, Satoshi Ikeda, Ryo Okuda, Tomohisa Baba, Shinichiro Iso, Koji Okudela, Tamiko Takemura, Shinji Sato, Takashi Ogura
Little has been reported on the characteristics of interstitial lung disease (ILD) associated with anti-citrullinated peptide/protein antibody (ACPA)-positive anti-synthetase syndrome (ASS). We sought to investigate the clinical, radiologic, and pathologic features of patients with ILD associated with ACPA-positive ASS. Medical records of seven ILD patients with positive results of both ACPA and anti-aminoacyl-tRNA synthetase antibody were retrospectively reviewed. Five patients had clinical symptoms associated with ASS other than ILD...
October 2018: Journal of Thoracic Disease
Danielle M Gerlag, Mary Safy, Karen I Maijer, Man Wai Tang, Sander W Tas, Mirian J F Starmans-Kool, Astrid van Tubergen, Matthijs Janssen, Maria de Hair, Monika Hansson, Niek de Vries, Aeilko H Zwinderman, Paul P Tak
OBJECTIVES: We explored the effects of B-cell directed therapy in subjects at risk of developing autoantibodypositive rheumatoid arthritis (RA), who never experienced inflammatory arthritis before, and explored biomarkers predictive of arthritis development. METHODS: Individuals positive for both anti-citrullinated peptide antibodies and rheumatoid factor but without arthritis were included in a randomised, double-blind, placebo-controlled study to receive a single infusion of 1000 mg rituximab or placebo...
December 1, 2018: Annals of the Rheumatic Diseases
Vikram Haridas, Kiran Haridas
Aim: To evaluate the prevalence of anti-thyroid antibodies in a cohort of rheumatoid arthritis (RA) patients from south India and their clinical use. Methods: A cross-sectional epidemiological study was conducted including 1217 patients aged more than 14 years with fever and thrombocytopenia admitted in the medical wards from October 2013 to September 2014. Detailed clinical examination and routine investigations were done; specific investigations like blood culture, widal test, antigen test for malaria, IgM ELISA leptospira, IgM ELISA dengue, bone marrow aspiration/biopsy etc...
May 2018: Journal of the Association of Physicians of India
Oladapo A Adewuya, Rasaaq A Adebayo, Adeola I Ajibade, Gbenga J Odunlami, Anthony O Akintomide, Suraj A Ogunyemi, Olufemi E Ajayi, Adebola O Adetiloye, Adeleye D Omisore, Oladipo A Olanipekun, Adeyinka O Owolabi, Ifeoluwa Amjo, Olumide A Akinyele, Abayomi O Bamgboje, Michael O Balogun
Background: Mixed connective tissue disease (MCTD; also known as Sharp's syndrome) is a rare autoimmune inflammatory disorder characterized by high titer of U1 ribonucleoprotein (U1RNP) antibody and clinical and serological overlap of systemic lupus erythematosus, systemic sclerosis, and polymyositis. The diagnosis is based on clinical and serological factors in criteria such as Alarcon-Segovia, Khan, Kusakawa, and Sharps. Cardiac disease can be a complication of connective tissue disease (CTD)...
2018: International Medical Case Reports Journal
Yan Xia, Jingli Song, Jia Feng, Rui Tian, Yinhao Zeng, Yang Xiang, Yufang Dai, Lin Yuan
Objective To investigate the association of single nucleotide polymorphisms (SNPs) of signal transducer and activator of transcription 4 (STAT4) rs7574865 and miRNA146a rs2910164 with rheumatoid arthritis (RA) in Wuling mountain area. Methods 287 RA patients and 305 age-matched healthy controls were included. The rs7574865 and rs2910164 SNPs of RA patients and control subjects were measured by multiplex PCR combined with high throughput sequencing(HI-SNP). The distributions of genotype and allele frequencies in the two groups were analyzed by the Chi-squared test, and also the relationship between these two SNPs and the risk of RA, rheumatoid factor (RF) and anti-cyclic citrullinated peptide (ACCP) antibody were investigated...
September 2018: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
Elizabeth Olivares-Martínez, Diego F Hernández-Ramírez, Carlos A Núñez-Álvarez, Luis Llorente, Gabriela Hernandez-Molina
OBJECTIVES: Although anti-cyclic citrullinated peptides antibodies are specific markers for rheumatoid arthritis (RA), they might be present in other diseases. Our aim was to assess the native or citrullinated antigens recognised by patients with primary Sjögren's syndrome (pSS) and to evaluate their association with clinical and serological features. METHODS: In an initial screening, we assessed the serum reactivity of 12 patients with pSS against native or in vitro citrullinated antigens of HEp-2 cells by immunoblotting...
November 8, 2018: Clinical and Experimental Rheumatology
Adrien Mirouse, Raphaèle Seror, Eric Vicaut, Xavier Mariette, Maxime Dougados, Anne-Laure Fauchais, Alban Deroux, Azeddine Dellal, Nathalie Costedoat-Chalumeau, Guillaume Denis, Jérémie Sellam, Jean-Benoît Arlet, Christian Lavigne, Geoffrey Urbanski, Dominique Fischer-Dumont, Abdou Diallo, Olivier Fain, Arsène Mékinian
OBJECTIVE: To describe the characteristics and the outcome of primary Sjögren Syndrome (pSS) associated arthritis and to compare the efficacy of different therapeutic regimen. METHODS: We conducted a retrospective study using Club Rhumatisme and Inflammation (CRI) and French Internal Medicine Society (SNFMI) networks. All patients with a diagnosis of pSS and at least one episode of clinical and/or echographic synovitis were included. Patients with synovitis (cases) were compared to pSS patients without synovitis (controls)...
November 5, 2018: Autoimmunity Reviews
Ankit Mahendra, Xingyu Yang, Shaza Abnouf, Jay R T Adolacion, Daechan Park, Sanam Soomro, Jason Roszik, Cristian Coarfa, Gabrielle Romain, Keith Wanzeck, S Louis Bridges, Amita Aggarwal, Peng Qiu, Sandeep K Agarwal, Chandra Mohan, Navin Varadarajan
OBJECTIVE: To obtain the comprehensive transcriptome profile of human citrulline-specific B cells from patients diagnosed with rheumatoid arthritis (RA). METHODS: Citrulline and hemagglutinin (HA) specific B cells were flow-sorted using peptide-streptavidin conjugates from peripheral blood of RA patients and healthy individuals respectively. Transcriptome profile of the sorted cells was obtained by RNA-sequencing, and expression of key protein molecules was evaluated by aptamer-based SOMAscan assay and flow cytometry...
November 8, 2018: Arthritis & Rheumatology
Monika Ponikowska, Jerzy Świerkot, Beata Nowak, Lucyna Korman, Piotr Wiland
OBJECTIVES: The aim of the study was to evaluate the frequency of anti-mutated citrullinated vimentin antibodies (a-Sa), anti-citrullinated α-enolase peptide 1 antibodies (a-CEP-1), anti-filaggrin antibodies (AFAs), heterogeneous nuclear ribonucleoprotein compies/anti-RA33-antibodies (a-hnRNP/RA33), anti-carbamylated protein antibodies (a-CarP), and metalloproteinase (MMPs) activity in patients with early inflammatory arthritis (EIA). METHODS: Seventy-four patients with EIA: 51 diagnosed with RA (rheumatoid arthritis) and 23 with UA (undifferentiated arthritis), and 20 healthy volunteers were enrolled to the study...
November 7, 2018: Clinical Rheumatology
Cliff Rims, Hannes Uchtenhagen, Mariana J Kaplan, Carmelo Carmona-Rivera, Philip Carlucci, Katalin Mikecz, Adrienn Markovics, Jeffrey Carlin, Jane H Buckner, Eddie A James
OBJECTIVE: Recognition of citrullinated antigens such as vimentin, fibrinogen, and alpha-enolase is associated with rheumatoid arthritis (RA). Emerging data suggests that the matrix protein aggrecan is also recognized as a citrullinated antigen. The goal of this study was to directly visualize cit-aggrecan specific T cells and characterize them in subjects with RA. METHODS: Citrullinated aggrecan peptides with likely DRB1*04:01 binding motifs were predicted using a previously published scanning algorithm...
November 2, 2018: Arthritis & Rheumatology
Martin Cornillet, Fruzsina Babos, Anna Magyar, Mireille Sebbag, Evelyne Verrouil, Ferenc Hudecz, Guy Serre, Leonor Nogueira
Quantification of Abs toward a single epitope is critical to understanding immunobiological processes. In autoimmunity, the prognostic value of the serological profiles of patients draws much attention, but the detection of Abs toward a single epitope is not well controlled. Particularly, the rheumatoid arthritis (RA)-specific anti-citrullinated protein/peptide Abs (ACPA) are specific to a two-atom change on arginyl residues and are considered a heterogeneous family of Abs. As a model, we studied ACPA to decipher how peptide features used as immunosorbent impact Ab detection...
December 1, 2018: Journal of Immunology: Official Journal of the American Association of Immunologists
José Pedro L Nunes, André C Cunha, Tiago Meirinhos, Alzira Nunes, Paulo M Araújo, Ana R Godinho, Eduardo M Vilela, Carlos Vaz
The prevalence of auto-antibodies associated to pulmonary arterial hypertension in scleroderma patients was reviewed, based on reports cited in two major scientific databases. Data were collected on the following types of antibodies: antinuclear, anti-double-stranded DNA, anticentromere, anti-CENP-A, anti-CENP-B, anti-bicaudal D2, anti-nucleolar, anti-Scl-70 (anti-topoisomerase I), anti-topoisomerase II α, anti-RNP, anti-U1RNP, anti-U3RNP, anti-RNA polymerase III, anti-Th/To, anti-histone, antiphospholipid, anti-PmScl, anti-Sm, anti SSA (anti-Ro),anti SSB (La), anti-Ro52 (TRIM 21), anti-Ku, anti-B23, anti-RuvBL1, anti-RuvBL2, anti-fibrin bound tissue plasminogen activator, anti-endothelial cell, anti-phosphatidylserine-prothrombin complex, anti-endothelin-1 type A receptor, anti-angiotensin II type 1 receptor, anti‑carbonic anhydrase II, anti-fibroblast, anti-cyclic citrullinated peptide, anti-4-sulfated N-Acetyl-lactosamine, class I and II anti-human leukocyte antigen...
December 2018: Autoimmunity Reviews
Viviane Malmström, Johanna Steen, Lars Klareskog
Rikard Holmdahl has commented on our recently published paper on specificity and function of human monoclonal antibodies (mAbs) from synovial plasma cells that react with citrullinated proteins and peptides (1). He also comments on our previous study from 2013 (2) that was based on mAbs generated from synovial B cells. Dr Holmdahl's letter provides us with an opportunity to discuss and clarify some methodological issues concerning RA monoclonal autoantibody expression, specificity, and functionality. In our eyes, cloning and characterization of human monoclonal autoantibodies from patients represents an important approach to the understanding of origins and functions of autoantibodies in the pathogenesis of immune-mediated diseases...
October 10, 2018: Arthritis & Rheumatology
G Belakova, V Manka, E Zanova, P Racay
Background: Anti-citrullinated peptides antibodies (ACPA) are specific for rheumatoid arthritis and have been implicated in disease pathogenesis. ACPA examination is a new component of ACR/EULAR 2010 classification criteria for rheumatoid arthritis. ACPA positivity predicts a more erosive disease course with severe joint damage and extra-articular manifestations. Objectives: To evaluate the benefits of ACPA examination in patients with early undifferentiated arthritis and patients with rheumatoid arthritis...
October 2018: Nigerian Journal of Clinical Practice
Lianne P W M Lelieveldt, Hendy Kristyanto, Ger J M Pruijn, Hans Ulrich Scherer, René E M Toes, Kimberly M Bonger
Autoreactive B cells are thought to play a pivotal role in many autoimmune diseases. Rheumatoid arthritis (RA) is an autoimmune disease affecting ∼1% of the Western population and is hallmarked by the presence of anticitrullinated proteins antibodies (ACPA) produced by autoreactive B cells. We intend to develop a method to target and selectively eliminate these autoreactive B cells using a sequential antigen prodrug targeting strategy. As ACPA-expressing B cells are thought to play essential roles in RA-disease pathogenesis, we used this B cell response as a prototype to analyze the feasibility to generate a construct consisting of a biologically silenced, that is, blocked, antigen connected to a cytotoxic prodrug...
October 26, 2018: Molecular Pharmaceutics
Kosuke Shabana, Nami Okamoto, Yuko Sugita, Keisuke Shindo, Takuji Murata, Hiroshi Tamai, Kenta Fujiwara
OBJECTIVE: We researched the findings of musculoskeletal ultrasound sonography (MSUS) on primary Sjogren's syndrome in childhood (pSS-C) with articular manifestations. The correlation of rheumatoid factor (RF) and anti-cyclic citrullinated peptide antibody (ACPA) were investigated to evaluate the usefulness of MSUS on their articular prognosis. METHOD: The objective patients are pSS-C cases who visited our hospital complaining joint pain and/or joint swelling and for whom MSUS was performed...
October 5, 2018: Modern Rheumatology
Hoshimi Kawaguchi, Isao Matsumoto, Atsumu Osada, Izumi Kurata, Hiroshi Ebe, Yuki Tanaka, Asuka Inoue, Naoto Umeda, Yuya Kondo, Hiroto Tsuboi, Akihito Ishigami, Takayuki Sumida
OBJECTIVE: To explore the relevance of citrullinated proteins and anti-citrullinated protein antibodies (ACPA) via protein arginine deiminase (PAD) inhibition in peptide glucose-6-phosphate isomerase-induced arthritis (pGIA). METHODS: Cl-amidine, a PAD inhibitor, was injected into pGIA. Clinical scores and histopathological findings of ankle joints were assessed. Serum ACPA titers were analyzed using ELISA. Citrullinated protein expression in joints and sera were examined with immunohistochemistry and Western blot analysis, respectively...
October 4, 2018: Modern Rheumatology
Kristine Germar, Cynthia M Fehres, Hans U Scherer, Nathalie van Uden, Sabrina Pollastro, Nataliya Yeremenko, Monika Hansson, Priscilla F Kerkman, Ellen I H van der Voort, Evan Reed, Hanna Maassen, Mark J Kwakkenbos, Arjen Q Bakker, Lars Klareskog, Vivianne Malmström, Niek de Vries, René E M Toes, Karin Lundberg, Hergen Spits, Dominique L Baeten
OBJECTIVE: Anti-citrullinated protein antibodies (ACPA) are a hallmark for rheumatoid arthritis (RA). Autoantigen-specific B-cell function aside from autoantibody production remains poorly understood in the context of this disease. We set out to elucidate this function through the isolation and immortalization of unique citrullinated protein or peptide (CP)-reactive B-cell clones from RA patients. METHODS: B-cell clones from either blood or synovial fluid of cyclic-citrullinated peptide 2 (CCP2)+ RA patients were immortalized by genetic reprogramming with Bcl6 and Bcl-xL...
October 1, 2018: Arthritis & Rheumatology
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