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Claudin 1

Naoki Oyama, Katarzyna Winek, Priscilla Bäcker-Koduah, Tian Zhang, Claudia Dames, Martina Werich, Olivia Kershaw, Christian Meisel, Andreas Meisel, Ulrich Dirnagl
Background and Purpose: The gut communicates with the brain bidirectionally via neural, humoral and immune pathways. All these pathways are affected by acute brain lesions, such as stroke. Brain-gut communication may therefore impact on the overall outcome after CNS-injury. Until now, contradictory reports on intestinal function and translocation of gut bacteria after experimental stroke have been published. Accordingly, we aimed to specifically investigate the effects of transient focal cerebral ischemia on intestinal permeability, gut associated lymphoid tissue and bacterial translocation in an exploratory study using a well-characterized murine stroke model...
2018: Frontiers in Neurology
Kai Zhang, Yuanlu Tu, Lipeng Gao, Meijuan Meng, Yunfeng Bai
BACKGROUND: The effect of soybean hull feeding on the disruption of colonic epithelium barrier function was investigated in goats fed a high-concentrate diet. Twenty-one Boer goats (live weight, 32.57 ± 2.26 kg; age, 1 year) were randomly divided into three groups: low-concentrate diet (LC), high-concentrate diet (HC), and high-concentrate diet with soybean hulls (SH). RESULTS: We found that the rumen fluid in the LC and SH group shown a higher pH value compared with the HC group...
December 3, 2018: BMC Veterinary Research
Lingbiao Xin, Ran Zhao, Jing Lei, Jianchan Song, Lin Yu, Ru Gao, Chuanbo Ha, Yuanyuan Ren, Xin Liu, Yixin Liu, Zhi Yao, Jie Yang
Staphylococcal nuclease domain-containing protein 1 (SND1) has been reported as an oncoprotein in a variety of cancers involving multiple processes, including proliferation, angiogenesis, and metastasis. However, the mechanisms underlying metastasis remain largely unknown. Herein, by using the ovarian cancer cell line SKOV3, which has high metastasis ability, we showed that loss-of-function of SND1 dramatically suppressed the invasion and migration of SKOV3 cells. We then performed gene expression profiles and further verified (by use of quantitative PCR and Western blot analysis) that loss-of-function of SND1 resulted in up-regulation of epithelial markers, such as epithelial cadherin and claudin 1, and down-regulation of mesenchymal markers, including neural cadherin and vimentin...
December 3, 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
Yi-Feng Zheng, Jie Luo, Guo-Lian Gan, Wei Li
Colorectal carcinoma (CRC) is a major cause of cancer-related deaths worldwide, and investigations on novel targets are imperative. MiR-98 has been reported to act as a tumor suppressor in several cancers. To evaluate miR-98 as a novel anticancer molecule for CRC, examinations to validate whether miR-98 conferred an inhibiting effect on proliferation, migration, and invasion were performed. The microarray-based gene expression profiling involving CRC was used to identify the differentially expressed genes. The potential relationship between miR-98 and CLDN1 was analyzed by cell experimentation...
December 2, 2018: Journal of Cellular Biochemistry
Nikola Sladojevic, Svetlana M Stamatovic, Allison M Johnson, Jennifer Choi, Anna Hu, Sophie Dithmer, Ingolf E Blasig, Richard F Keep, Anuska V Andjelkovic
Recent evidence suggests that blood brain barrier (BBB) recovery and reestablishment of BBB impermeability after stroke is incomplete. This could influence stroke recovery, increase the risk of repeat stroke and be a solid substrate for developing vascular dementia. While accumulating evidence has defined morphological alterations and underlying mechanisms of tight junction (TJ) changes during BBB breakdown in acute stroke, very little is known about the type of alterations and mechanisms in BBB "leakage" found sub-acutely or chronically...
November 30, 2018: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
Derek Sunil Nowrangi, Devin McBride, Anatol Manaenko, Brandon Dixon, Jiping Tang, John H Zhang
Disruption of the blood-brain barrier results in the formation of edema and contributes to the loss of neurological function following intracerebral hemorrhage (ICH). This study examined insulin-like growth factor-1 (IGF-1) as a treatment and its mechanism of action for protecting the blood-brain barrier after ICH in mice. 171 Male CD-1 mice were subjected to ICH via collagenase or autologous blood. A dose study for recombinant human IGF-1 (rhIGF-1) was performed. Brain water content and behavioral deficits were evaluated at 24 and 72 h after the surgery, and Evans blue extravasation and hemoglobin assay were conducted at 24 h...
November 29, 2018: Experimental Neurology
Cesar D Solís, Teresa Morales
Diminished sensitivity to kainic acid (KA) lesioning action is present in the hippocampus of the lactating dam. Given that KA can alter blood-brain barrier (BBB) permeability, we investigated whether lactation would protect BBB permeability after treatment with this neurotoxin. Adult virgin or lactating (13-15 days pp) female Wistar rats were injected with an i.p. (10 mg/kg b.w.) or i.c.v. (100 ng/1 μl) KA dose. Control groups received a similar volume of vehicle. BBB permeability was assessed by an injection of fluorescent FITC-conjugated dextran tracer (40 kDa, 50 mg/500 μl) or of 4% Evans blue dye (500 μl), given 24 h after KA administration...
November 28, 2018: Brain Research Bulletin
Iqbal Sayeed, Nefize Turan, Donald G Stein, Bushra Wali
Because vitamin D hormone deficiency (VDHdef ) can worsen severity and outcome for ischemic stroke, we examined the role of VDH in maintaining blood-brain-barrier (BBB integrity) in a rat model of stroke. In most types of stroke, the BBB is markedly compromised, potentially leading to a cascade of injury processes and functional deficits, so we examined a number of biomarkers associated with BBB disruption to determine whether VDH deficiency would further compromise the BBB following a stroke. Male Wistar rats were randomly assigned to one of two diet cohorts, VDH-sufficient (VDHsuf ) and VDHdef ...
November 28, 2018: Experimental Neurology
Yonglin Zhao, Xing Wei, Jinning Song, Ming Zhang, Tingqin Huang, Jie Qin
Our early experiments confirmed that rosiglitazone (RSG), a peroxisome proliferator-activated receptor γ (PPARγ) agonist, had therapeutic potential for the treatment of diffuse axonal injury (DAI) by inhibiting the expression of amyloid-beta precursor protein and reducing the loss and abnormal phosphorylation of tau, but the underlying mechanisms were not fully defined. In this study, we aimed to investigate a possible role for PPARγ in the protection of blood-brain barrier (BBB) integrity in a rat model of DAI, and the underlying mechanisms...
November 28, 2018: Inflammation
Jason C Gallagher, Michael J Satlin, Abdulrahman Elabor, Nidhi Saraiya, Erin K McCreary, Esther Molnar, Claudine El-Beyrouty, Bruce M Jones, Deepali Dixit, Emily L Heil, Kimberly C Claeys, Jon Hiles, Nikunj M Vyas, Christopher M Bland, Jin Suh, Kenneth Biason, Dorothy McCoy, Madeline A King, Lynette Richards, Nicole Harrington, Yi Guo, Saira Chaudhry, Xiaoning Lu, Daohai Yu
Background: Multidrug-resistant Pseudomonas aeruginosa infections remain common in hospitals worldwide. We investigated the outcomes associated with the use of ceftolozane-tazobactam for the treatment of these infections. Methods: Data were collected retrospectively from 20 hospitals across the United States about adults who received ceftolozane-tazobactam for the treatment of multidrug-resistant P aeruginosa infections of any source for at least 24 hours. The primary outcome was a composite of 30-day and inpatient mortality, and secondary outcomes were clinical success and microbiological cure...
November 2018: Open Forum Infectious Diseases
Jennifer M Mongiovi, Gary R Zirpoli, Rikki Cannioto, Lara E Sucheston-Campbell, Dawn L Hershman, Joseph M Unger, Halle C F Moore, James A Stewart, Claudine Isaacs, Timothy J Hobday, Muhammad Salim, Gabriel N Hortobagyi, Julie R Gralow, G Thomas Budd, Kathy S Albain, Christine B Ambrosone, Susan E McCann
BACKGROUND: The pathophysiology of chemotherapy-induced peripheral neuropathy (CIPN) is not well understood. Currently, dose reduction is the only recommendation for alleviating symptoms, often leading to premature treatment cessation. The primary aim of this analysis was to determine the association between components of diet during taxane treatment for breast cancer and change in CIPN symptoms over treatment. METHODS: Women with stage II or III invasive breast cancer were enrolled into an ancillary study to the North American Breast Cancer Intergroup phase III trial (S0221) led by the Southwest Oncology Group (SWOG)...
November 28, 2018: Breast Cancer Research: BCR
Eugene Roscioli, Rhys Hamon, Susan E Lester, Hubertus P A Jersmann, Paul N Reynolds, Sandra Hodge
BACKGROUND: Individuals with respiratory disease are being increasingly exposed to wildfire smoke as populations encroach further into forested regions and climate change continues to bring higher temperatures with lower rainfall. Frequent exposures have significant potential to accelerate conditions such as chronic obstructive pulmonary disease (COPD) which is characterised by an exaggerated inflammatory response to environmental stimuli. Here we employ models of human airway epithelium exposed to wildfire smoke-extract (WFSE) to examine modulation in airway epithelial cell (AEC) survival, fragility and barrier function...
November 28, 2018: Respiratory Research
Li Zou, Ke Liu, Hui-Feng Zhu, Shan Feng
This paper aimed to observe the protective effect of catalpol on the high glucose induced destruction of tight junctions of rat primary brain microvascular endothelial cells (BMECs). Catalpol co-administrated with high glucose increased BMECs survival, decreased its ET-1 secretion, and improved transmembrane electrical resistance in a time-dependent manner. Furthermore, transmission electron microscopy was used to observe catalpol's protective effect on tight junction. Fluorescence staining displayed that catalpol reversed the rearrangement of the cytoskeleton protein F-actin and up-regulated the tight junction proteins claudin-5 and ZO-1, which were further demonstrated by the mRNA expression levels of claudin-5, occludin, ZO-1, ZO-2, ZO-3, α -actintin, vinculin and cateinins...
October 2018: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
Claudine Angela Blum, Philipp Schuetz, Nicole Nigro, Bettina Winzeler, Birsen Arici, Julie Refardt, Sandrine Andrea Urwyler, Nicolas Rodondi, Manuel Raphael Blum, Matthias Briel, Beat Mueller, Mirjam Christ-Crain
OBJECTIVE: Glucocorticoids have been shown to improve outcome in community-acquired pneumonia (CAP). However, glucocorticoids have potential side-effects, and treatment response may vary. It is thus crucial to select patients with high likelihood to respond favorably. In critical illness, cosyntropin testing is recommended to identify patients in need for glucocorticoids. We investigated whether consyntropin testing predicts treatment response to glucocorticoids in CAP. DESIGN: PREDEFINED SECONDARY ANALYSIS OF A RANDOMIZED CONTROLLED TRIAL: PATIENTS: HOSPITALIZED PATIENTS WITH CAP: MEASUREMENTS: We performed 1μg cosyntropin tests in a randomized trial comparing prednisone 50mg for seven days to placebo...
November 28, 2018: Clinical Endocrinology
Xiaoxiao Fan, Shuai Li, Zhenlong Wu, Zhaolai Dai, Ju Li, Xiaolong Wang, Guoyao Wu
This study was conducted to test the hypothesis that preweaning  glycine supplementation to breast-fed piglets alleviated the post-weaning  apoptosis of jejunal epithelium through CHOP signaling. Seven-day-old sow-reared piglets were orally administrated with 0, 50, 100, or 200% of glycine intake from sow's milk twice daily for 14 days and then were weaned at 21 days of age. Tissue samples were collected at 28 days of age for determining intestinal morphology, serum diamine oxidase (DAO) activity, abundances of proteins involved in ER stress and apoptosis...
November 28, 2018: Amino Acids
Nicole Meyers, Carol Nelson-Williams, Laura Malaga-Dieguez, Horacio Kaufmann, Erin Loring, James Knight, Richard P Lifton, Howard Trachtman
Hypokalemia of renal origin can arise from genetic abnormalities in a variety of transporters or channel proteins that mediate tubular handling of potassium. Recently, mutations in claudin 10 have been documented in patients with hypokalemia in association with a range of other electrolyte abnormalities and skin and sweat gland manifestations. We report a 12-year-old Hispanic boy who presented with anhydrosis, aptyalism, alacrima, hypokalemia, and hypocalciuria, in whom we detected a homozygous mutation in the claudin 10 gene...
October 25, 2018: American Journal of Kidney Diseases: the Official Journal of the National Kidney Foundation
Onur Gökhan Yildirim, Esra Sumlu, Esra Aslan, H Buğra Koca, M Bilgehan Pektas, Gökhan Sadi, Fatma Akar
The increased consumption of high-fructose in diet may contribute to high prevalence of metabolic syndrome in the world. The influence of high-fructose diet on male reproductive system has been poorly documented. In this study, we investigated the effects of dietary high-fructose on the expression of inflammatory cytokines in association with certain testicular proteins and sex hormones in the testis of rats. Fructose was given to the rats as 20% solution (7.8 mg/kg) in drinking water for 15 weeks. Dietary high-fructose caused testicular degeneration, also decreased testicular concentration of testosterone and right testis absolute weight...
November 27, 2018: Toxicology Mechanisms and Methods
Wei Wang, Weichun Yang, Ziyi Shen, Sixian Wen, Minyu Hu
To study the dose-response effect of lycopene on vessel and neuron damage in the brain against hyperlipidemia, rats were fed with hypercholesterolemic feed and treated with lycopene orally by gavage at the dose of 5, 25, 45, 65, 85, and 105, 125 mg/kg/bw-1/d-1. At the end of the fourth week, lycopene doses and serum lycopene concentration showed an inverse U-shape curve. Serum lycopene concentration was negatively correlated with the levels of serum TC, TG, LDL-C, as well as the cerebral LDL-C, VEGF, and VCAM-1; Serum lycopene concentration was positively correlated with the expression of Claudin-5 and the number of neurons in hippocampal CA1 and CA3...
November 26, 2018: Journal of Agricultural and Food Chemistry
Stephen Saikiu Shaida, Judith Sophie Weber, Thaddeus Terlumun Gbem, Sen Claudine Henriette Ngomtcho, Usman Baba Musa, Mbunkha Daniel Achukwi, Mohammed Mamman, Iliya Shehu Ndams, Jonathan Andrew Nok, Soerge Kelm
BACKGROUND: Tsetse flies are vectors of trypanosomes, parasites that cause devastating disease in humans and livestock. In the course of vector control programmes it is necessary to know about the Glossina species present in the study area, the population dynamics and the genetic exchange between tsetse fly populations. RESULTS: To achieve an overview of the tsetse fly diversity in Nigeria and at the Nigeria-Cameroon border, tsetse flies were trapped and collected between February and March 2014 and December 2016...
November 23, 2018: BMC Microbiology
Lin Zhang, Xiuying Wang, Shaokui Chen, Shuhui Wang, Zhixiao Tu, Guolong Zhang, Huiling Zhu, Xiangen Li, Jianglin Xiong, Yulan Liu
This study was conducted to investigate whether medium-chain triglycerides (MCTs) attenuated lipopolysaccharide (LPS)-induced liver injury by down-regulating necroptotic and inflammatory signaling pathways. A total of 24 pigs were randomly allotted to four treatments in a 2 × 2 factorial design including diet (0 and 4% MCTs) and immunological challenge (saline and LPS). After three weeks of feeding with or without 4% MCTs, pigs were challenged with saline or LPS. MCTs led to a significant increase in eicosapentaenoic acid, docosahexaenoic acid and total (n-3) polyunsaturated fatty acid concentrations...
November 21, 2018: International Journal of Molecular Sciences
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