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Rodolfo G Gatto, Weiguo Li, Jin Gao, Richard L Magin
Diffusion magnetic resonance imaging (MRI) exhibits contrast that identifies macro- and microstructural changes in neurodegenerative diseases. Previous studies have shown that MR diffusion tensor imaging (DTI) can observe changes in spinal cord white matter in animals and humans affected with symptomatic amyotrophic lateral sclerosis (ALS). The goal of this preclinical work was to investigate the sensitivity of DTI for the detection of signs of tissue damage before symptoms appear. High-field MRI data were acquired using a 9...
August 2018: NMR in Biomedicine
Magdalena Koziorowska-Gilun, Przemysław Gilun, Katarzyna Mietelska, Władysław Kordan
The key prerequisite for successful insemination is sperm characterized to have positive values for morphological and biological variables which are determined by, among others, effective antioxidant protection during the lifespan of sperm cells. This study evaluated the activity and relative abundance of mRNA for antioxidant enzymes in stallion testicular and epididymal tissues during breeding (n = 5) and non-breeding (n = 5) seasons. The activity of superoxide dismutase (SOD) was greater (P < 0...
August 11, 2018: Animal Reproduction Science
Ryan S Atlasi, Ravinder Malik, Christian I Corrales, Laura Tzeplaeff, Julian P Whitelegge, Neil R Cashman, Gal Bitan
Mutations in Cu/Zn-superoxide dismutase (SOD1) gene are linked to 10-20% of familial amyotrophic lateral sclerosis (fALS) cases. The mutations cause misfolding and self-assembly of SOD1 into toxic oligomers and aggregates, resulting in motor neuron degeneration. The molecular mechanisms underlying SOD1 aggregation and toxicity are unclear. Characterization of misfolded SOD1 is particularly challenging due to its metastable nature. Antibodies against misfolded SOD1 are useful tools for this purpose, provided their specificity and selectivity are well characterized...
August 15, 2018: ACS Chemical Biology
Makito Hirano, Makoto Samukawa, Chiharu Isono, Kazumasa Saigoh, Yusaku Nakamura, Susumu Kusunoki
Objective: To assess the contribution of noncoding repeat expansions in Japanese patients with amyotrophic lateral sclerosis (ALS). Methods: Sporadic ALS in Western countries is frequently associated with noncoding repeat expansions in the C9ORF72 gene. Spinocerebellar ataxia type 8 (SCA8) is another noncoding repeat disease caused by expanded CTA/CTG repeats in the ATXN8OS gene. Although the involvement of upper and lower motor neurons in SCA8 has been reported, a positive association between SCA8 and ALS remains unestablished...
August 2018: Neurology. Genetics
Zhe Li, ZhenZhen Xiong, Lydia C Manor, Hongbao Cao, Tao Li
Recent studies have reported hundreds of genes linked to Alzheimer's Disease (AD). However, many of these candidate genes may be not identified in different studies when analyses were replicated. Moreover, results could be controversial. Here, we proposed a computational workflow to curate and evaluate AD related genes. The method integrates large scale literature knowledge data and gene expression data that were acquired from postmortem human brain regions (AD case/control: 31/32 and 22/8). Pathway Enrichment, Sub-Network Enrichment, and Gene-Gene Interaction analysis were conducted to study the pathogenic profile of the candidate genes, with 4 metrics proposed and validated for each gene...
July 2018: Saudi Journal of Biological Sciences
Behnam Ahmadipour, Hossein Hassanpour, Fariborz Khajali
BACKGROUND: Fatness is an unwanted side effect of genetic selection in broiler chickens. In this study, we introduce mountain celery powder as a feed supplement to suppress lipogenesis and improve antioxidant status in broiler chickens. Male broiler chicks (Ross 308) were fed a control diet or a diet that includes mountain celery (MC) at 7.5 gkg-1 over 42 days. RESULTS: Body weight gain and feed conversion ratio significantly (P < 0.05) improved in chicks fed MC...
August 13, 2018: BMC Veterinary Research
Eduardo C Alexandre, Fabiano B Calmasini, Amanda C da S Sponton, Mariana G de Oliveira, Diana M André, Fábio H Silva, Maria Andréia Delbin, Fabíola Z Mónica, Edson Antunes
Obese mice display overactive bladder (OAB) associated with impaired urethra smooth muscle (USM) function. In this study, we evaluated the role of the adipose tissue surrounding the urethra and prostate in obese mice (here referred as periprostatic adipose tissue; PPAT) to the USM dysfunction. Male C57BL6/JUnib mice fed with either a standard-chow or high-fat diet to induce obesity were used. In PPAT, histological analysis, and qPCR analysis for gp91phox, tumor necrosis factor-α (TNF-α) and superoxide dismutase (SOD) were conducted...
August 10, 2018: European Journal of Pharmacology
Hu Hua, Xuhua Ge, Mengqiu Wu, Chunhua Zhu, Lihong Chen, Guangrui Yang, Yue Zhang, Songming Huang, Aihua Zhang, Zhanjun Jia
BACKGROUND/AIMS: In clinic, excessive acetaminophen (APAP) can cause kidney damage with uncertain mechanisms. Recently, accumulating evidence demonstrated a pathogenic role of mitochondrial dysfunction in the kidney injury. Thus, in this study, rotenone, a mitochondrial complex I inhibitor, was applied to the mice with APAP-induced acute kidney injury to evaluate the effect of mitochondrial complex I inhibition on APAP nephrotoxicity. METHODS: After 3 days of rotenone pretreatment, mice were administered with APAP (300mg/kg) by intraperitoneal injection for 24 h...
August 10, 2018: Kidney & Blood Pressure Research
Xi Chen, Jinku Yan, Fan He, Dongyan Zhong, Huilin Yang, Ming Pei, Zong-Ping Luo
Mesenchymal stem cells (MSCs) are promising cell sources for regenerative medicine. Growing evidence has indicated that mechanical stimuli are crucial for their lineage-specific differentiation. However, the effect of mechanical loading on redox balance and the intracellular antioxidant system in MSCs was unknown. In this study, human bone marrow-derived MSCs (BM-MSCs) were subjected to cyclic stretch at the magnitude of 2.5%, 5%, and 10%. Cell proliferation, intracellular reactive oxygen species (ROS), expression of antioxidant enzymes, and osteogenic differentiation were evaluated...
August 7, 2018: Free Radical Biology & Medicine
Shi Shu, Xingxing Lei, Fang Liu, Bo Cui, Qing Liu, Qingyun Ding, Ming Sheng Liu, Xiao Guang Li, Liying Cui, Xue Zhang
NEK1 was recently identified as an amyotrophic lateral sclerosis (ALS) gene through rare variant burden analysis, and its role in ALS in various populations is still unclear. The aim of this study was to determine the frequency and spectrum of NEK1 mutations in an ALS cohort from mainland China. All exons and their flanking intron regions of NEK1 were screened by direct nucleotide sequencing in 377 unrelated ALS patients. These patients were also screened with a massive parallel sequencing gene panel for 24 known ALS genes and C9orf72 hexanucleotide repeat expansion...
June 28, 2018: Neurobiology of Aging
Giovanni Nardo, Maria Chiara Trolese, Mattia Verderio, Alessandro Mariani, Massimiliano de Paola, Nilo Riva, Giorgia Dina, Nicolò Panini, Eugenio Erba, Angelo Quattrini, Caterina Bendotti
BACKGROUND: The major histocompatibility complex I (MHCI) is a key molecule for the interaction of mononucleated cells with CD8+ T lymphocytes. We previously showed that MHCI is upregulated in the spinal cord microglia and motor axons of transgenic SOD1G93A mice. METHODS: To assess the role of MHCI in the disease, we examined transgenic SOD1G93A mice crossbred with β2 microglobulin-deficient mice, which express little if any MHCI on the cell surface and are defective for CD8+ T cells...
August 9, 2018: Molecular Neurodegeneration
Fusheng Zhao, Fang Lei, Xiang Yan, Senfeng Zhang, Wen Wang, Yu Zheng
BACKGROUND/AIMS: Cigarette smoke exposure (CSE) during pregnancy is a well-recognized health hazard that causes placental damage. Hydrogen sulfide (H2S) has been reported to protect multiple organs from injury. However, the protective effects of H2S have not been tested in the placenta. This study aimed to explore the potential of H2S in protecting placenta against oxidative injury induced by CSE during pregnancy and the possible underlying mechanisms. METHODS: Pregnant SD rats were randomly divided into 4 groups: NaCl, NaHS (a donor of H2S), CSE and CSE+NaHS...
August 9, 2018: Cellular Physiology and Biochemistry
Umer Javed Butt, Syed Adnan Ali Shah, Touqeer Ahmed, Saadia Zahid
Lead (Pb), a ubiquitous heavy metal and a known neurotoxicant, produces adverse effects on the brain via increased production of reactive oxygen species (ROS) and causes oxidative stress. In this study we examined the neuroprotective effects of the ethanolic extract of Nigella sativa L. seeds on Pb induced oxidative stress in the developing brain of mice. Mouse pups were exposed to low (0.1%) and high (0.2%) doses of Pb from the first day of pregnancy through their mothers ( via drinking water) and lactation until post-natal day (PND) 21...
January 1, 2018: Toxicology Research
Andrea Stoccoro, Lorena Mosca, Vittoria Carnicelli, Ugo Cavallari, Christian Lunetta, Alessandro Marocchi, Lucia Migliore, Fabio Coppedè
AIM: To investigate mitochondrial DNA (mtDNA) copy number and D-loop region methylation in carriers of SOD1, TARDBP, FUS and C9orf72 mutations. METHODS: Investigations were performed in blood DNA from 114 individuals, including amyotrophic lateral sclerosis (ALS) patients, presymptomatic carriers and noncarrier family members. RESULTS: Increased mtDNA copy number (p = 0.0001) was observed in ALS patients, and particularly in those with SOD1 or C9orf72 mutations...
August 8, 2018: Epigenomics
Xiao-Long Qi, Jing Wang, Hong-Yuan Yue, Shu-Geng Wu, Ya-Nan Zhang, He-Min Ni, Yong Guo, Hai-Jun Zhang, Guang-Hai Qi
The objective of this study consisting of 2 trials was to investigate the antioxidant role of conjugated linoleic acid (CLA) isomers (c9, t11-CLA and t10, c12-CLA) and the underlying mechanism by which they act in modulating redox status in a primary laying hen hepatocyte culture. In trial 1, the cytotoxicity of CLA isomers or linoleic acid (LA) (0, 25, 50, 100, 200, 400, 800 μmol/L) was evaluated by 3-(4,5-dimethylthiazol-2-yl)- 2,5-diphenyltetrazolium bromide (MTT) assay. The concentration of CLA isomers or LA (25, 50, 100 μmol/L) for proper antioxidant activity was evaluated by measuring the antioxidant enzyme activity...
August 1, 2018: Poultry Science
Sudhir Jain, Anita Rana, Kavita Jain, Sravan K Perla, Nitin Puri, Ashok Kumar
Background: The contribution of single nucleotide polymorphisms in transcriptional regulation of the human angiotensin receptor type I (hAT1R) gene in age related chronic pathologies such as hypertension and associated renal disorders is not well known. The hAT1R gene has SNPs in its promoter that forms two haplotypes (Hap), Hap-I and Hap-II. Hap-I of AT1R gene is associated with hypertension in Caucasians. We have hypothesized here that age will alter the transcriptional environment of the cell and will regulate the expression of hAT1R gene in a haplotype-dependent manner...
August 1, 2018: American Journal of Hypertension
L Zeng, N Chen, Q Ning, Y Yao, H Chen, R Dang, H Zhang, C Lei
With the proposed global climate change, heat tolerance is becoming increasingly important to the sustainability of livestock production systems. Results from previous studies showed that variants in the prolactin releasing hormone (PRLH) (AC_000160.1:g.11764610G>A) and superoxide dismutase 1 (SOD1) (AC_000158.1:g.3116044T>A) genes play an important role in heat tolerance in African indicine cattle. However, it is unknown whether or not the mutations are associated with heat tolerance in Chinese cattle...
August 5, 2018: Animal Genetics
Amrit Kumar Shrestha, Renuka T Menon, Binoy Shivanna
Hyperoxia-induced oxidative stress contributes to the pathogenesis of bronchopulmonary dysplasia (BPD), the most common respiratory morbidity of preterm infants. Importantly, the disease lack specific therapies and is associated with long-term cardio-pulmonary and neurodevelopmental morbidities, signifying the need to discover novel therapies and decrease the disease burden. We and others have demonstrated that leflunomide, a food and drug administration approved drug to treat humans with rheumatoid arthritis, increases the expression of the anti-oxidant enzymes, NAD(P)H quinone dehydrogenase 1 (NQO1), catalase, and superoxide dismutase (SOD)...
August 1, 2018: Biochemical and Biophysical Research Communications
Carmen Rodríguez-Cueto, Irene Santos-García, Laura García-Toscano, Francisco Espejo-Porras, MLuz Bellido, Javier Fernández-Ruiz, Eduardo Muñoz, Eva de Lago
Antioxidant phytocannabinoids, synthetic compounds targeting the CB2 receptor, and inhibitors of the endocannabinoid inactivation afforded neuroprotection in SOD1G93A mutant mice, a model of ALS. These effects may involve the activation of PPAR-γ too. Here, we have investigated the neuroprotective effects in SOD1G93A mutant mice of the cannabigerol derivative VCE-003.2, which works as neuroprotectant by activating PPAR-γ. Mice were treated with VCE-003.2 from 60 days up to an advanced stage in disease progression (18 weeks), when they were euthanized and used for analysis of neuropathological signs...
August 1, 2018: Biochemical Pharmacology
Fabrizio D'Ovidio, James P K Rooney, Anne E Visser, Umberto Manera, Ettore Beghi, Giancarlo Logroscino, Roel C H Vermeulen, Jan Herman Veldink, Leonard H van den Berg, Orla Hardiman, Adriano Chiò
OBJECTIVES: Several studies focused on the association between alcohol consumption and amyotrophic lateral sclerosis (ALS), although with inconsistent findings. Antioxidants may play a role since lyophilised red wine was found to prolong SOD1 mice lifespan. The aim of this international population-based case-control study performed in Ireland, The Netherlands and Italy was to assess the role of alcohol, and red wine in particular, in developing ALS. METHODS: Euro-MOTOR is a case-control study where patients with incident ALS and controls matched for gender, age and area of residency were recruited in a population-based design...
August 3, 2018: Journal of Neurology, Neurosurgery, and Psychiatry
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