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urinary cell excretion

Gaia C Mannino, Anastasia Fuoco, Maria A Marini, Rosangela Spiga, Concetta Di Fatta, Elettra Mancuso, Francesco Perticone, Francesco Andreozzi, Giorgio Sesti
Previous studies suggested that the IGF-1/IGF-1 receptor signaling pathway may contribute to regulate uric acid levels. To confirm this hypothesis, we assessed the effects of the IGF-1-raising genetic variant rs35767 on urate levels in serum and urine, and we investigated IGF-1 ability to modulate the expression of transporters involved in reabsorption and secretion of uric acid in the kidney. The study population included 2794 adult Whites. 24-hour urinary uric acid concentration was available for 229 subjects...
August 16, 2018: Scientific Reports
Enzo R Russo, Inalda Facincani, Katia C Nakazato, Terezila M Coimbra, Eduardo J Crevelin, Ana Maria S Pereira, Fabio Carmona
Curcumin is a polyphenol present in the rhizomes of the species Curcuma longa L. ("turmeric," Zingiberaceae), which has been used for centuries as an anti-inflammatory. We aimed to evaluate the anti-inflammatory effects of C. longa in renal injury induced by doxorubicin (DOX, 3.5 IV). We studied four groups of Wistar rats: two groups with DOX-induced kidney injury, one fed with standard food and another with standard food mixed with C. longa (5 mg.g-1 ). Two other control groups without kidney injury were fed with the same foods...
August 15, 2018: Phytotherapy Research: PTR
Shanshan Guo, Gang Liu, David M Frazer, Tianqing Liu, Linhao You, Jiaqi Xu, Yongwei Wang, Gregory J Anderson, Guangjun Nie
Chelators are commonly used to remove excess iron in iron-loading disorders. Deferoxamine (DFO) is an effective and safe iron chelator but an onerous parenteral administration regimen limits its routine use. To develop more effective methods for delivering iron chelators, we examined whether amphiphilic copolymer nanoparticles (NPs) could deliver DFO more efficiently. Physical characterization showed a uniform and stable preparation of DFO nanoparticles (DFO-NPs) with an average diameter of 105.3 nm. In macrophage (RAW264...
August 9, 2018: Nano Letters
Natalia Chebotareva, Irina Bobkova, Lidia Lysenko, Natalia Neprinzeva, Anatoly Vinogradov, Sergey Moiseev
We evaluated the heat shock system 70 (HSP70) in patients with chronic glomerulonephritis (CGN). Seventy-six patients with CGN patients were included in our study. Ten patients with mild proteinuria (median 0.48 [0.16-0.78] g/24 h) and ten healthy subjects served as positive and negative controls, respectively. Urinary levels of HSP70, interleukin-10, and serum levels of anti-HSP70 were measured by ELISA. The immunohistochemical peroxidase method was used to study the expression of HSP70 and Foxp3+ in kidney biopsies...
July 30, 2018: Cell Stress & Chaperones
Yunxia Li, Zhiru Tang, Tiejun Li, C Chen, Feiruo Huang, Jing Yang, Qingqing Xu, Jifu Zhen, Zhaoliang Wu, Mao Li, Jiajing Sun, Jinchao Chen, Xiangxin Zhang, Liuting Wu, Rui An, Shengjun Zhao, Qingyan Jiang, Weiyun Zhu, Yulong Yin, Zhihong Sun
This study was performed to determine if pyruvate, which acts as a critical intermediate in energy metabolism, can substitute the role of glutamate as a metabolic fuel and effectively reduce nitrogen excretion in pigs. First, the experiment in vitro was carried out to investigate the effects of culturing porcine small intestinal epithelial cell line with pyruvate on the oxidation. Then, barrows weighing 40 kg were used in the experiment investigating the changes of nitrogen balance in response to addition of pyruvate to low-protein diets...
July 27, 2018: Journal of Animal Science
Stine Julie Tingskov, Tae-Hwan Kwon, Jørgen Frøkiær, Rikke Nørregaard
Lithium is widely used in the treatment of bipolar affective disorders, but often causes nephrogenic diabetes insipidus (NDI), a condition characterized by a severe urinary concentrating defect. Lithium-induced NDI is associated with dysregulation of the amiloride-sensitive epithelial sodium channel (ENaC), which is essential for renal sodium reabsorption. Sex hormones have been shown to affect the expression of aquaporin-2 (AQP2) and sodium transporters. Therefore, we evaluated whether tamoxifen (TAM), a selective estrogen receptor modulator (SERM), would affect lithium-induced dysregulation of ENaC subunits and natriuresis...
2018: Frontiers in Physiology
Matthias B Moor, Barbara Haenzi, Finola Legrand, Robert Koesters, Nancy E Hynes, Olivier Bonny
Ablation of the Mediator of ErbB2-driven Cell Motility 1 (Memo1) in mice altered calcium homeostasis and renal calcium transporter abundance by an unknown mechanism. Here, we investigated the role of intrarenal Memo in renal calcium handling. We have generated a mouse model of inducible kidney-specific Memo1 deletion. The Memo-deficient mice showed normal serum concentration and urinary excretion of calcium and phosphate, but elevated serum FGF23 concentration. They displayed elevated gene expression and protein abundance of the distal renal calcium transporters NCX1, TRPV5, and calbindin D28k...
2018: Frontiers in Physiology
Chao-Jung Chen, Wen-Ling Liao, Chiz-Tzung Chang, Hsin-Yi Liao, Fuu-Jen Tsai
Diabetic nephropathy (DN) is one of the most common complications in diabetic patients. New noninvasive markers are still needed for the early detection of DN before identifiable alternations in kidney function or urine albumin excretion occurs. A C18 plate and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) were used to compare the urinary protein profiles of 238 subjects from the following 4 groups: patients with type 2 diabetic (T2D) with microalbuminuria, patients with DM without micro- or macroalbuminuria, patients with micro- or macroalbuminuria due to nondiabetic disease, and healthy controls...
2018: PloS One
C Saponaro, F Pattou, C Bonner
With the increasing prevalence of type 2 diabetes (T2D), therapies aimed at delineating diabetes pathophysiology and understanding their mechanisms of action are of critical importance. As such, growing interest in the clinical pharmacology of sodium-glucose cotransporter 2 (SGLT2) and its inhibition by gliflozins in the treatment of T2D is becoming increasingly evident. SGLT2 inhibition results in urinary glucose excretion, thereby reducing blood glucose levels. The importance of this homoeostasis mechanism is evident from several clinical trials demonstrating that patients taking this class of compounds have reductions in glycaemia, body weight and blood pressure compared with other antidiabetic agents...
June 30, 2018: Diabetes & Metabolism
Vanessa Gómez-Vallejo, María Puigivila, Sandra Plaza-García, Boguslaw Szczupak, Rafael Piñol, José L Murillo, Victor Sorribas, Gustavo Lou, Sabino Veintemillas, Pedro Ramos-Cabrer, Jordi Llop, Angel Millán
In vitro experiments have shown the great potential of magnetic nanocarriers for multimodal imaging diagnosis and non-invasive therapies. However, their extensive clinical application is still jeopardized by a fast retention in the reticuloendothelial system (RES). The other issue that restrains their potential performance is slow degradation and excretion, which increases their risks of toxicity. We report a promising case in which multicore iron oxide nanoparticles coated with a poly(4-vinylpyridine) polyethylene glycol copolymer show low RES retention and high urinary excretion, as confirmed by single photon emission computerized tomography (SPECT), gamma counting, magnetic resonance imaging (MRI) and electron microscopy (EM) biodistribution studies...
August 7, 2018: Nanoscale
Divya Bhatia, Aditi Sinha, Pankaj Hari, Shailaja Sopory, Savita Saini, Mamta Puraswani, Himanshi Saini, Dipendra K Mitra, Arvind Bagga
BACKGROUND: Rituximab, a monoclonal antibody targeting B lymphocytes, effectively sustains remission in steroid-dependent nephrotic syndrome (SDNS). We studied its effects on lymphocyte subsets and urinary CD80 excretion (uCD80) in patients with SDNS. METHODS: Blood and urine samples were collected from 18 SDNS patients before rituximab, and after 1 month and 1 year or at first relapse. T and B lymphocytes and uCD80 were determined by flow cytometry and ELISA, respectively...
July 9, 2018: Pediatric Research
Ali C M Johnson, Richard A Zager
BACKGROUND: Recent clinical data support the utility/superiority of a new AKI biomarker ("NephroCheck"), the arithmetic product of urinary TIMP × IGFBP7 concentrations. However, the pathophysiologic basis for its utility remains ill defined. METHODS: To clarify this issue, CD-1 mice were subjected to either nephrotoxic (glycerol, maleate) or ischemic AKI. Urinary TIMP2/IGFBP7 concentrations were determined at 4 and 18 hours postinjury and compared with urinary albumin levels...
August 2018: Journal of the American Society of Nephrology: JASN
Philipp Opitz, Olf Herbarth
BACKGROUND: Different organisms such as bacteria, molds and humans produce and release a relative unknown class of metabolites which are responsible for the individual olfactory pattern. These volatile organic metabolites (VOM) represent a kind of biosignature that reflects the sum of all multifactorial influences, including genetics, environmental factors, nutritional and disease status. As a result of pathological processes the individual body odor can be influenced by newly produced or altered compositions of the VOM...
July 3, 2018: Journal of Otolaryngology—Head & Neck Surgery
Sophie Moog, Sophie Moog, Sébastien Houy, Elodie Chevalier, Stéphane Ory, Georges Weryha, Marion Rame, Marc Klein, Laurent Brunaud, Stéphane Gasman, Thomas Cuny
<br>Background: 18F-FDOPA positron emission tomography/computed tomography (PET/CT) is a sensitive nuclear imaging for the diagnosis of pheochromocytomas (PHEO). However, its utility as a predictive factor of the secretion of catecholamines remains poorly studied. METHODS: Thirty-nine histologically-confirmed PHEO were included in this retrospective monocentric study. Patients underwent 18F-FDOPA PET/CT before surgery with evaluation of several uptake parameters (SUVmax, SUVmean and the metabolic burden [MB] calculated as follows: MB = SUVmean x tumor volume) and measurement of plasma and/or urinary metanephrine (MN), normetanephrine (NM) and chromogranin A (CGA)...
June 27, 2018: Neuroendocrinology
Davoud Sanajou, Amir Ghorbani Haghjo, Hassan Argani, Leila Roshangar, Saeed Nazari Soltan Ahmad, Zahra Ashrafi Jigheh, Somayeh Aslani, Fatemeh Panah, Jalil Rashedi, Mehran Mesgari Abbasi
Despite the effectiveness of renin-angiotensin blockade in retarding diabetic nephropathy progression, a considerable number of patients still develop end-stage renal disease. The present investigation aims to evaluate the protective potential of FPS-ZM1, a selective inhibitor of receptor for advanced glycation end products (RAGE), alone and in combination with valsartan, an angiotensin receptor blocker, against glomerular injury parameters in streptozotocin-induced diabetic rats. FPS-ZM1 at 1 mg/kg (i.p.), valsartan at 100 mg/kg (p...
June 14, 2018: Journal of Physiology and Biochemistry
Hui Guo, Faping Li, Weiguo Xu, Jinjin Chen, Yuchuan Hou, Chunxi Wang, Jianxun Ding, Xuesi Chen
Initially, chemotherapy is effective for treatment of bladder cancer after transurethral resection of the bladder. However, certain patients progressively become unresponsive after multiple treatment cycles, which results from the rapid and almost complete excretion of clinically used formulations of antineoplastic agents with urinary voiding. Improving the mucoadhesiveness and penetrability of chemotherapeutic drugs are key factors in treatment of advanced bladder cancer. Here, a smart disulfide-crosslinked polypeptide nanogel of poly(l-lysine)-poly(l-phenylalanine- co -l-cystine) (PLL-P(LP- co -LC)) is developed to deliver 10-hydroxycamptothecin (HCPT) for treatment of orthotopic bladder cancer...
June 2018: Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
Toshifumi Nakamura, Isao Kurihara, Sakiko Kobayashi, Kenichi Yokota, Ayano Murai-Takeda, Yuko Mitsuishi, Mitsuha Morisaki, Nao Kohata, Yosuke Oshima, Yukiko Minami, Hirotaka Shibata, Hiroshi Itoh
BACKGROUND: Mineralocorticoid receptor (MR) has pathological roles in various cell types, including renal tubule cells, myocytes, and smooth muscle cells; however, the role of MR in intestinal epithelial cells (IECs) has not been sufficiently evaluated. The intestine is the sensing organ of ingested sodium; accordingly, intestinal MR is expected to have essential roles in blood pressure (BP) regulation. METHODS AND RESULTS: We generated IEC-specific MR knockout (IEC-MR-KO) mice...
June 21, 2018: Journal of the American Heart Association
Dan-Dan Zhang, Zhong-Xiuzi Gao, Carlos P Vio, Yu Xiao, Peng Wu, Hao Zhang, Xi-Wen Guo, Xin-Xin Meng, Li Gu, Jun-Lin Wang, Xin-Peng Duan, Dao-Hong Lin, Wen-Hui Wang, Ruimin Gu
Stimulation of BK2R (bradykinin [BK] B2 receptor) has been shown to increase renal Na+ excretion. The aim of the present study is to explore the role of BK2R in regulating Kir4.1 and NCC (NaCl cotransporter) in the distal convoluted tubule (DCT). Immunohistochemical studies demonstrated that BK2R was highly expressed in both apical and lateral membrane of Kir4.1-positive tubules, such as DCT. Patch-clamp experiments demonstrated that BK inhibited the basolateral 40-pS K+ channel (a Kir4.1/5.1 heterotetramer) in the DCT, and this effect was blocked by BK2R antagonist but not by BK1R (BK B1 receptor) antagonist...
August 2018: Hypertension
Yoshio Ogura, Munehiro Kitada, Itaru Monno, Keizo Kanasaki, Ai Watanabe, Daisuke Koya
OBJECTIVES: Mitochondrial oxidative stress is involved in the pathogenesis of diabetic kidney disease. The objective of our study is to identify the mechanisms of renal mitochondrial oxidative stress, focusing on Sirt3, which is nicotinamide adenine dinucleotide (NAD+ ; oxidized NAD)-dependent deacetylase in mitochondria. METHODS: Renal mitochondrial oxidative stress and Sirt3 activity, using Zucker diabetic fatty rats (ZDFRs) and cultured proximal tubular cells under high-glucose condition were evaluated...
December 2018: Redox Report: Communications in Free Radical Research
Weihua Li, Hongqun Zheng, Jiankai Xu, Shaodong Cao, Xiuan Xu, Peng Xiao
OBJECTIVES: c-Met is a receptor tyrosine kinase shown inappropriate expression and actively involved in progression and metastasis in most types of human cancer. Development of c-Met-targeted imaging and therapeutic agents would be extremely useful. Previous studies reported that c-Met-binding peptide (Met-pep1, YLFSVHWPPLKA) specifically targets c-Met receptor. Here, we evaluated 18F-labeled Met-pep1 for PET imaging of c-Met positive tumor in human head and neck squamous cell carcinoma (HNSCC) xenografted mice...
2018: PloS One
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