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David B Chapel, Aliya N Husain, Thomas Krausz
Accurate distinction of benign mesothelial proliferations from malignant mesothelioma remains a diagnostic challenge. Sequential use of BAP1 immunohistochemistry and CDKN2A fluorescence in situ hybridization is specific for diagnosis of mesothelioma, but fluorescence in situ hybridization is both costly and time-consuming. Early data indicate that mesothelioma shows extensive loss of nuclear 5-hydroxymethylcytosine (5-hmC). We studied 49 cases of mesothelioma (17 epithelioid mesothelioma, 22 biphasic mesothelioma, and 10 sarcomatoid mesothelioma) and 23 benign mesothelial proliferations using a 5-hmC single immunohistochemical stain, CAM5...
October 12, 2018: Modern Pathology: An Official Journal of the United States and Canadian Academy of Pathology, Inc
Shinya Toyokuni
Cancer is the primary cause of human mortality in most countries. This tendency has increased as various medical therapeutics have advanced, which suggests that we cannot escape carcinogenesis, although the final outcome may be modified by exposomes and statistics. Cancer is classified by its cellular differentiation. Mesothelial cells are distinct in that they line somatic cavities, facilitating the smooth movement of organs, but are not exposed to the external environment. Malignant mesothelioma, or simply mesothelioma, develops either in the pleural, peritoneal or pericardial cavities, or in the tunica vaginalis testes...
October 9, 2018: Free Radical Biology & Medicine
Ryuichiro Kimura, Azusa Yoneshige, Man Hagiyama, Tomoyuki Otani, Takao Inoue, Naoki Shiraishi, Kazuyoshi Yanagihara, Tomohiko Wakayama, Akihiko Ito
AIMS: To determine cellular distribution of cell adhesion molecule 1 (CADM1), an immunoglobulin superfamily member, in the human oxyntic gastric mucosa, and to explore possible involvement in the development and peritoneal dissemination of signet ring cell (SRC) gastric carcinoma, which often develops in the oxyntic mucosa. MAIN METHODS: Immunohistochemistry and double immunofluorescence were conducted on surgical specimens of normal and SRC-bearing stomachs and peritoneal metastatic foci of SRCs...
October 9, 2018: Life Sciences
Satomi Funahashi, Yasumasa Okazaki, Takahiro Nishiyama, Hidekazu Ohyoshi, Hiroyuki Yasui, Kazuki Nishida, Shigeyuki Matsui, Shinya Toyokuni
Exposure to asbestos fiber is central to mesothelial carcinogenesis, for which iron overload in or near mesothelial cells is a key pathogenic mechanism. Alternatively, iron chelation therapy with deferasirox or regular phlebotomy was significantly preventive against crocidolite-induced mesothelial carcinogenesis in rats. However, the role of iron transporters during asbestos-induced carcinogenesis remains elusive. Here, we studied the role of divalent metal transporter 1 (DMT1; Slc11a2), which is a Fe(II) transporter, that is present not only on the apical plasma membrane of duodenal cells but also on the lysosomal membrane of every cell, in crocidolite-induced mesothelial carcinogenesis using DMT1 transgenic (DMT1Tg) mice...
October 12, 2018: Free Radical Research
Fatéméh Dubois, Bastien Jean-Jacques, Hélène Roberge, Magalie Bénard, Ludovic Galas, Damien Schapman, Nicolas Elie, Didier Goux, Maureen Keller, Elodie Maille, Emmanuel Bergot, Gérard Zalcman, Guénaëlle Levallet
BACKGROUND: By allowing intercellular communication between cells, tunneling nanotubes (TNTs) could play critical role in cancer progression. If TNT formation is known to require cytoskeleton remodeling, key mechanism controlling their formation remains poorly understood. METHODS: The cells of human bronchial (HBEC-3, A549) or mesothelial (H2452, H28) lines are transfected with different siRNAs (inactive, anti-RASSF1A, anti-GEFH1 and / or anti-Rab11). At 48 h post-transfection, i) the number and length of the nanotubes per cell are quantified, ii) the organelles, previously labeled with specific tracers, exchanged via these structures are monitored in real time between cells cultured in 2D or 3D and in normoxia, hypoxia or in serum deprivation condition...
October 11, 2018: Cell Communication and Signaling: CCS
Monalisa Hui, K R Harshavardhana, Shantveer G Uppin
Pericardial mesotheliomas are rare tumors which often present with features of constrictive pericarditis. We present clinical, imaging, histological, and immunohistochemical findings of three cases presenting with chronic constrictive pericarditis. Two of these cases were initially treated as tuberculous pericarditis. Histologically, all the three cases were of an epithelioid type and showed positivity for more than one mesothelial markers. Two patients had a fatal outcome, and one was lost to follow-up.
October 2018: Indian Journal of Pathology & Microbiology
Jorge Cabral Ribeiro, Luciana Sousa, Vicente Gonçalves
The adenomatoid tumors are rare benign mesothelial lesions that in males affect mainly the epididymis. They account for over one third of paratesticular tumors, and the treatment of choice has been surgical excision. The origin in testicular parenchyma or the tunica albuginea is extremely rare. Clinical signs and imaging studies are often inconclusive in differentiate from a more common malignant intratesticular solid tumour, which can result in unnecessary orchiectomies. We present a case of adenomatoid tumor of tunica albuginea where clinical suspicion and intraoperative frozen section analysis led to a partial orchiectomy...
April 2018: Revista Internacional de Andrología
Asudan Tugce Bozkurter Cil, Ilhami Oguzhan Aydogdu
BACKGROUND: We aimed to evaluate the effect of fat grafting on postoperative intraabdominal adhesions by promoting mesothelial healing on a rat model. METHODS: Thirty Wistar albino rats were grouped as control and fat grafted group. They all underwent laparotomy, anterior cecal wall abrasion and peritoneal injury. On postoperative day 14, all surviving rats were sacrificed and adhesions, fibrosis and inflammation were graded using quantitative scoring systems. RESULTS: Fat grafting significantly reduced adhesion formation compared to the control group (p < 0...
September 28, 2018: Archives of Medical Research
Andrew Churg, Kazuki Nabeshima, Greta Ali, Rossella Bruno, Lynnette Fernandez-Cuesta, Francoise Galateau-Salle
OBJECTIVES: The separation of benign from malignant mesothelial proliferations and exact subclassification of mesothelioma subtypes is crucial to determining patient care and prognosis but morphologically can be very difficult. METHODS: This session of the 2018 IMIG meeting addressed these problems. RESULTS: A new immunohistochemical marker, methylthioadenosine phosphorylase, was shown to correlate well with CDKN2A FISH and is cheaper and faster to run...
October 2018: Lung Cancer: Journal of the International Association for the Study of Lung Cancer
Mariantonieta Tirado, William Koss
No abstract text is available yet for this article.
September 27, 2018: Blood
Alexandra Helmke, Janis Casper, Johannes Nordlohne, Sascha David, Hermann Haller, Elisabeth Zeisberg, Sibylle von Vietinghoff
Endothelial cells can acquire a mesenchymal phenotype upon irritation [endothelial-to-mesenchymal transition (EndMT)]. Macrophages accumulate in the atherosclerotic plaque. This study addressed whether macrophages modulate EndMT and delineated a reciprocal effect of EndMT on macrophage functions in atherosclerosis. In atherosclerotic murine and human aortas, endothelial cells with mesenchymal markers were elevated by confocal microscopy and flow cytometric analysis. Increased EndMT master transcription factor Snai1 expression and extracellular matrix are consistent with enhanced EndMT in this condition...
September 27, 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
V Ascoli, B Murer, A Nottegar, C Luchini, R Carella, F Calabrese, F Lunardi, I Cozzi, L Righi
Malignant pleural mesothelioma is a neoplasm characterized by a very poor prognosis and medico-legal implications. Diagnosis, prognosis and therapy are often challenging and include several issues. Cytological diagnosis is frequently the first step of the diagnostic process, and although its sensitivity may be somewhat lower, diagnostic criteria should be taken into account. When effusion cytology is inconclusive for the diagnosis, tissue biopsies should be taken. Even if the morphologic criteria for deciding whether a mesothelial proliferation is a benign or a malignant process have been defined, the separation of benign from malignant mesothelial proliferation is often a difficult problem for the pathologist, particularly on small biopsies...
March 2018: Pathologica
Artur Filipe Rodrigues, Leon Newman, Dhifaf A Jasim, Isabella A Vacchi, Cécilia Ménard-Moyon, Livia E Crica, Alberto Bianco, Kostas Kostarelos, Cyrill Bussy
Graphene oxide (GO) is an oxidised form of graphene that has attracted commercial interest in multiple applications, including inks, printed electronics and spray coatings, which all raise health concerns due to potential creation of inhalable aerosols. Although a number of studies have discussed the toxicity of GO sheets, the in vivo impact of their lateral dimensions is still not clear. Here, we compared the effects of large GO sheets (l-GO, 1-20 µm) with those of small GO sheets (s-GO, < 1 µm) in terms of mesothelial damage and peritoneal inflammation, after intraperitoneal (i...
September 26, 2018: Archives of Toxicology
Michael S Balzer, Alexandra Helmke, Martina Ackermann, Janis Casper, Lei Dong, Marcus Hiss, Yulia Kiyan, Song Rong, Kai Timrott, Sibylle von Vietinghoff, Le Wang, Hermann Haller, Nelli Shushakova
Background: Peritoneal membrane (PM) damage during peritoneal dialysis (PD) is mediated largely by high glucose (HG)-induced pro-inflammatory and neo-angiogenic processes, resulting in PM fibrosis and ultrafiltration failure. We recently demonstrated a crucial role for protein kinase C (PKC) isoform α in mesothelial cells. Methods: In this study we investigate the role of PKCβ in PM damage in vitro using primary mouse peritoneal macrophages (MPMΦ), human macrophages (HMΦ) and immortalized mouse peritoneal mesothelial cells (MPMCs), as well as in vivo using a chronic PD mouse model...
September 21, 2018: Nephrology, Dialysis, Transplantation
Rita Carmona, Laura Ariza, Elena Cano, Manuel Jiménez-Navarro, Ramón Muñoz-Chápuli
Most animals develop coelomic cavities lined by an epithelial cell layer called the mesothelium. Embryonic mesothelial cells have the ability to transform into mesenchymal cells which populate many developing organs contributing to their connective and vascular tissues, and also to organ-specific cell types. Furthermore, embryonic mesothelium and mesothelial-derived cells produce essential signals for visceral morphogenesis. We review the most relevant literature about the mechanisms regulating the embryonic mesothelial-mesenchymal transition, the developmental fate of the mesothelial-derived cells and other functions of the embryonic mesothelium, such as its contribution to the establishment of left-right visceral asymmetries or its role in limb morphogenesis...
September 26, 2018: Seminars in Cell & Developmental Biology
Masanori Takahashi, Masaru Tamura, Shigeru Sato, Kiyoshi Kawakami
Omphalocele is a human congenital anomaly in ventral body wall closure and may be caused by impaired formation of the primary abdominal wall (PAW) and/or defects in abdominal muscle development. Here, we report that mice doubly deficient in homeobox genes Six4 and Six5 showed ventral body wall closure defects reproducing human omphalocele. SIX4 and SIX5 were localized in surface ectodermal cells and somatic mesoderm-derived mesenchymal and coelomic epithelial cells (CECs) in the PAW. Six4 -/- ; Six5 -/- fetuses exhibited large omphalocele with protrusion of both the liver and intestine or small omphalocele with that of the intestine with complete penetrance...
September 20, 2018: Disease Models & Mechanisms
Seiichi Funamoto, Yoshihide Hashimoto, Akio Kishida, Jun Negishi
As most surgical treatments pose a risk of tissue adhesion, methods to prevent adhesion are needed across various surgical fields. In this study, we investigated the use of a decellularized pericardium with fibrin glue to prevent rat heart adhesion. Porcine pericardia were decellularized by a high-hydrostatic pressure method. Cells adhered to the resulting pericardial extracellular matrix (ECM) during an in vitro cell-seeding test, but fibrin-coated pericardial ECM showed reduced cell adhesion. In a rat surgical model of heart adhesion, the fibrin-coated pericardial ECM did not adhere to the heart and mesothelial cell adhesion was observed on the ECM surface...
September 19, 2018: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
Subramanyam Dasari, Taruni Pandhiri, James Haley, Dean Lenz, Anirban K Mitra
Intercellular interactions play an important role in many biological processes, including tumor progression, immune responses, angiogenesis, and development. Paracrine or juxtacrine signaling mediates such interactions. The use of a conditioned medium and coculture studies are the most common methods to discriminate between these two types of interactions. However, the effect of localized high concentrations of secreted factors in the microenvironment during the paracrine interactions is not accurately recapitulated by conditioned medium and, thus, may lead to imprecise conclusions...
August 28, 2018: Journal of Visualized Experiments: JoVE
Ioannis Patoulias, Evangelia Rachmani, Maria Kalogirou, Kyriakos Chatzopoulos, Dimitrios Patoulias
The aim of this study is to describe an entity of primary hydrocele accompanied with fibrosis, thickening and hemorrhagic infiltration of parietal layer of tunica vaginalis (PLTV). During a 4-year period (2011-2014), 94 boys (2.5-14 years old) underwent primary hydrocele repair. Hydrocele was right sided in 55 (58.5 %), left sided in 26 (28.7%) and bilateral in 12 patients (13.8%). Eighty three out of 94 patients (88.30%) had communicating hydrocele and the rest eleven patients (11.7%) had non-communicating...
2018: Acta Medica (Hradec Králové)
David A Wolf, William Beeson, John D Rachel, Gregory S Keller, C William Hanke, Jill Waibel, Matt Leavitt, Michael Sacopulos
The use of stem cells in regenerative medicine and specifically facial rejuvenation is thought provoking and controversial. Today there is increased emphasis on tissue engineering and regenerative medicine, which translates into a need for a reliable source of stem cells in addition to biomaterial scaffolds and cytokine growth factors. Adipose tissue is currently recognized as an accessible and abundant source for adult stem cells. Cellular therapies and tissue engineering are still in their infancy, and additional basic science and preclinical studies are needed before cosmetic and reconstructive surgical applications can be routinely undertaken and satisfactory levels of patient safety achieved...
November 2018: Facial Plastic Surgery Clinics of North America
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