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Amp deaminase

Xin-Kai Zhang, Ming-Yue Nie, Jun Chen, Liu-Jing Wei, Qiang Hua
Lycopene has been broadly studied in recent decades due to its health benefits including cancer prevention, anti-atherogenic and anti-obesity effects, and modulation of the immune system. To obtain efficient synthesis of lycopene, extensive researches have been conducted in various microbial cells, including Yarrowia lipolytica, to heterologously produce lycopene using various genetic and metabolic engineering methods. In this study, the effects of copy numbers of lycopene synthesis genes, a variety of key central metabolic genes (especially AMP deaminase-encoding gene AMPD), and 5-L fermenter cultivation on lycopene production in Y...
November 15, 2018: Journal of Biotechnology
Anna Bin, Valentina Caputi, Michela Bistoletti, Monica Montopoli, Rocchina Colucci, Luca Antonioli, Sara De Martin, Ignazio Castagliuolo, Genny Orso, Cristina Giaroni, Patrizia Debetto, Maria Cecilia Giron
Adenosine is a versatile signaling molecule recognized to physiologically influence gut motor functions. Both the duration and magnitude of adenosine signaling in enteric neuromuscular function depend on its availability, which is regulated by the ecto-enzymes ecto-5'-nucleotidase (CD73), alkaline phosphatase (AP), and ecto-adenosine deaminase (ADA) and by dipyridamole-sensitive equilibrative transporters (ENTs). Our purpose was to assess the involvement of CD73, APs, ecto-ADA in the formation of AMP-derived adenosine in primary cultures of ileal myofibroblasts (IMFs)...
September 29, 2018: Purinergic Signalling
Linjie Wang, Zhe Chao, Yan Wang
The AMP deaminase 1 (AMPD1) gene plays an important role in purine nucleotide interconversion and energy metabolism. In this study, two novel single nucleotide polymorphisms (SNPs) (g.-626 G > A and g.-566 A>G) were detected in the proximal promoter region of the AMPD1 gene. The Chinese indigenous pig breeds (Meishan and Tongcheng) had higher frequencies of the G and A alleles than Western meat-type breeds (Landrace and Large White) at the g.-626 G > A and g.-566 A>G loci. The transcriptional activity of the AMPD1 promoter carrying the haplotype H1 (A-626 G-566 ) was significantly (p < 0...
November 2018: DNA and Cell Biology
M-N Theodoraki, T K Hoffmann, E K Jackson, T L Whiteside
Exosomes in plasma of head and neck squamous cell carcinoma (HNSCC) patients comprise subsets of vesicles derived from various cells. Recently, we separated CD3(+) from CD3(-) exosomes by immune capture. CD3(-) exosomes were largely tumour-derived (CD44v3+ ). Both subsets carried immunosuppressive proteins and inhibited functions of human immune cells. The role of these subsets in immune cell reprogramming by the tumour was investigated by focusing on the adenosine pathway components. Spontaneous adenosine production by CD3(+) or CD3(-) exosomes was measured by mass spectrometry, as was the production of adenosine by CD4+ CD39+ regulatory T cells (Treg ) co-incubated with these exosomes...
October 2018: Clinical and Experimental Immunology
Francesca Ronca, Antonio Raggi
Multiple muscle-specific isoforms of the Zn2+ metalloenzyme AMP deaminase (AMPD) have been identified based on their biochemical and genetic differences. Our previous observations suggested that the metal binding protein histidine-proline-rich glycoprotein (HPRG) participates in the assembly and maintenance of skeletal muscle AMP deaminase (AMPD1) by acting as a zinc chaperone. The evidence of a role of millimolar-strength phosphate in stabilizing the AMPD-HPRG complex of both AMPD1 and cardiac AMP deaminase (AMPD3) is suggestive of a physiological mutual dependence between the two subunit components with regard to the stability of the two isoforms of striated muscle AMPD...
August 23, 2018: Biomolecules
Shuai Yuan, Xiaofeng Jiang, Xinyu Zhou, Yuqing Zhang, Teng Teng, Peng Xie
Inosine, a major breakdown product of adenosine by the enzyme adenosine deaminase, binds to adenosine receptors and initiates intracellular signaling events. Adenosine and inosine have already been described as capable of modulating depression-like behavior in adult rodent model. In this study, we investigated the antidepressant-like effect of inosine, acutely administered (10 or 50 mg/kg, intraperitoneal), in postnatal day 28 male Sprague-Dawley rats. The effects of inosine were assessed using the forced swimming test and locomotor activity test...
September 26, 2018: Neuroreport
David F Wilson, Franz M Matschinsky
A model of oxidative phosphorylation and its regulation is presented that is consistent with the experimental data on metabolism in higher plants and animals. The variables that provide real time control of metabolic status are: intramitochondrial [NAD+ ]/[NADH], energy state ([ATP]/[ADP][Pi]), and local oxygen concentration ([O2 ]). ATP consumption and respiratory chain enzyme content are tissue specific (liver vs heart muscle) and the latter is modulated by chronic alterations in ATP consumption (i.e. endurance training etc)...
July 12, 2018: Journal of Applied Physiology
Naďa Špačková, Kamila Réblová
Adenosine to inosine (A⁻I) editing is the most common modification of double-stranded RNA (dsRNA). This change is mediated by adenosine deaminases acting on RNA (ADARs) enzymes with a preference of U>A>C>G for 5&prime; neighbor and G>C=A>U or G>C>U=A for 3&prime; neighbor. A⁻I editing occurs most frequently in the non-coding regions containing repetitive elements such as ALUs. It leads to disruption of RNA duplex structure, which prevents induction of innate immune response...
June 28, 2018: Genes
Debjit Das, Payel Das, Mahammed Moniruzzaman, Mousumi Poddar Sarkar, Joyita Mukherjee, Suman Bhusan Chakraborty
Current study aims to find interrelation between mitochondrial enzyme function and fatty acid profile in fish muscle and role of antioxidant agents to maintain their balance in response to metal accumulation. Fishes (Labeo rohita, Catla catla, Cirrhinus cirrhosus) were collected from two sites (Nalban Bheri and Diamond Harbour, India). Concentrations of metals (lead, cadmium, copper, nickel, zinc), enzymatic and non-enzymatic antioxidant activity (malondialdehyde, superoxide dismutase, catalase, glutathione reductase, glutathione peroxidase, glutathione S-transferase), muscle enzyme activity (acetylcholinesterase, succinate dehydrogenase, lactate dehydrogenase, Ca2+ ATPase, AMP-deaminase, lipoamide reductase, cytochrome C oxidase, aldolase) and fatty acid composition in muscle tissues were analyzed...
September 2018: Chemosphere
I M Rybakowska, B Kutryb-Zając, R Milczarek, B Łukasz, E M Slominska, R T Smolenski
Nucleotide metabolism plays a major role in a number of vital cellular processes such as energetics. This, in turn, is important in pathologies such as atherosclerosis. Three month old atherosclerotic mice with knock outs for LDLR and apolipoprotein E (ApoE) were used for the experiments. Activities of AMP-deaminase (AMPD), ecto5'-nucleotidase (e5NT), adenosine deaminase (ADA), purine nucleoside phosphorylase (PNP) were measured in heart, liver and kidney cortex and medulla by analysing conversion of substrates into products using HPLC...
2018: Nucleosides, Nucleotides & Nucleic Acids
Timothy F Cloughesy, Joseph Landolfi, Michael A Vogelbaum, Derek Ostertag, James B Elder, Stephen Bloomfield, Bob Carter, Clark C Chen, Steven N Kalkanis, Santosh Kesari, Albert Lai, Ian Y Lee, Linda M Liau, Tom Mikkelsen, Phioanh Nghiemphu, David Piccioni, William Accomando, Oscar R Diago, Daniel J Hogan, Dawn Gammon, Noriyuki Kasahara, Thian Kheoh, Douglas J Jolly, Harry E Gruber, Asha Das, Tobias Walbert
Background: Vocimagene amiretrorepvec (Toca 511) is an investigational gamma-retroviral replicating vector encoding cytosine deaminase that, when used in combination with extended-release 5-fluorocytosine (Toca FC), results preclinically in local production of 5-fluorouracil, depletion of immune-suppressive myeloid cells, and subsequent induction of antitumor immunity. Recurrent high-grade glioma (rHGG) patients have a high unmet need for effective therapies that produce durable responses lasting more than 6 months...
September 3, 2018: Neuro-oncology
Yuki Tatekoshi, Masaya Tanno, Hidemichi Kouzu, Koki Abe, Takayuki Miki, Atsushi Kuno, Toshiyuki Yano, Satoko Ishikawa, Wataru Ohwada, Tatsuya Sato, Takeshi Niinuma, Hiromu Suzuki, Tetsuji Miura
AMP deaminase (AMPD) plays a crucial role in adenine nucleotide metabolism. Recently we found that upregulated AMPD activity is associated with ATP depletion and contractile dysfunction under the condition of pressure overloading in the heart of a rat model of type 2 diabetes mellitus (T2DM), OLETF. Here we examined the mechanism of AMPD upregulation by T2DM. The protein level of 90-kDa full-length AMPD3 was increased in whole myocardial lysates by 55% in OLETF compared to those in LETO, a non-diabetic control...
June 2018: Journal of Molecular and Cellular Cardiology
Yu Wang, Ye Liu, Jiao Liu, Yanmei Guo, Liwen Fan, Xiaomeng Ni, Xiaomei Zheng, Meng Wang, Ping Zheng, Jibin Sun, Yanhe Ma
CRISPR/Cas9 or Cpf1-introduced double strand break dramatically decreases bacterial cell survival rate, which hampers multiplex genome editing in bacteria. In addition, the requirement of a foreign DNA template for each target locus is labor demanding and may encounter more GMO related regulatory hurdle in industrial applications. Herein, we developed a multiplex automated Corynebacterium glutamicum base editing method (MACBETH) using CRISPR/Cas9 and activation-induced cytidine deaminase (AID), without foreign DNA templates, achieving single-, double-, and triple-locus editing with efficiencies up to 100%, 87...
May 2018: Metabolic Engineering
Debjit Das, Mahammed Moniruzzaman, Soumalya Mukhopadhyay, Samya Karan, Adity Sarbajna, Suman Bhusan Chakraborty
Present study investigates the effect of metal accumulation on antioxidant level and mitochondrial enzymes function in muscle of Oreochromis mossambicus. Metal accumulation in muscle upregulated stress marker malondialdehyde and the activity of different antioxidant enzymes with no significant alteration in glutathione system. Metal exposure to fish muscle decreased the activity of mitochondrial enzymes. AMP deaminase, aldolase, cytochrome C oxidase and lipoamide reductase showed positive correlation with acetylcholinesterase, glutathione reductase, reduced glutathione and glutathione peroxidase, but negative correlation with superoxide dismutase, catalase, glutathione S-transferase and thiobarbituric acid reactive substance...
May 2018: Bulletin of Environmental Contamination and Toxicology
Amy E Pasquinelli
Adenosine deaminases that act on RNA (ADARs) convert adenosines (A) to inosines (I) in stretches of dsRNA. The biological purpose of these editing events for the vast majority of ADAR substrates is largely unknown. In this issue of Genes & Development, Reich and colleagues (pp. 271-282) demonstrate that in Caenorhabditis elegans , A-to-I editing in double-stranded regions of protein-coding transcripts protects these RNAs from targeting by the RNAi pathway. Disruption of this safeguard through loss of ADAR activity coupled with enhanced RNAi results in developmental abnormalities and profound changes in gene expression that suggest aberrant induction of an antiviral response...
February 1, 2018: Genes & Development
Aline da Silva Pereira, Lizielle Souza de Oliveira, Thauan Faccin Lopes, Jucimara Baldissarelli, Taís Vidal Palma, Mayara Sandrielly Pereira Soares, Luiza Spohr, Vera Maria Morsch, Cinthia Melazzo de Andrade, Maria Rosa Chitolina Schetinger, Roselia Maria Spanevello
Diabetes Mellitus (DM) is associated with an increased susceptibility to various infections, which might be attributed to changes in immune response owing to chronic hyperglycemia. Nucleoside triphosphate diphosphohydrolase (NTPDase), 5'-nucleotidase, and adenosine deaminase (ADA) are important enzymes involved in the generation of anti-aggregant and anti-inflammatory microenvironments. The aim of this study was to evaluate the effect of gallic acid (GA) on the hematological parameters and ectonucleotidase activities in platelets, lymphocytes, and serum of diabetic rats...
May 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Travis Nemkov, Kaiqi Sun, Julie A Reisz, Anren Song, Tatsuro Yoshida, Andrew Dunham, Matthew J Wither, Richard O Francis, Robert C Roach, Monika Dzieciatkowska, Stephen C Rogers, Allan Doctor, Anastasios Kriebardis, Marianna Antonelou, Issidora Papassideri, Carolyn T Young, Tiffany A Thomas, Kirk C Hansen, Steven L Spitalnik, Yang Xia, James C Zimring, Eldad A Hod, Angelo D'Alessandro
Hypoxanthine catabolism in vivo is potentially dangerous as it fuels production of urate and, most importantly, hydrogen peroxide. However, it is unclear whether accumulation of intracellular and supernatant hypoxanthine in stored red blood cell units is clinically relevant for transfused recipients. Leukoreduced red blood cells from glucose-6-phosphate dehydrogenase-normal or -deficient human volunteers were stored in AS-3 under normoxic, hyperoxic, or hypoxic conditions (with oxygen saturation ranging from <3% to >95%)...
February 2018: Haematologica
Jing Wu, Qiuling Luo, Jia Liu, Xiulai Chen, Liming Liu
OBJECTIVES: To enhance acid tolerance of Candida glabrata for pyruvate production by engineering AMP metabolism. RESULTS: The physiological function of AMP deaminase in AMP metabolism from C. glabrata was investigated by deleting or overexpresseing the corresponding gene, CgAMD1. At pH 4, CgAMD1 overexpression resulted in 59 and 51% increases in biomass and cell viability compared to those of wild type strain, respectively. In addition, the intracellular ATP level of strain Cgamd1Δ/CgAMD1 was down-regulated by 22%, which led to a 94% increase in pyruvate production...
January 2018: Biotechnology Letters
Renata S Pereira, Claudia M Bertoncheli, Stephen A Adefegha, Lívia G Castilhos, Karine L Silveira, João Felipe P Rezer, Pedro H Doleski, Fátima H Abdalla, Karen F Santos, Claudio A M Leal, Roberto C V Santos, Emerson A Casali, Cesar E J Moritz, Daniel R Stainki, Daniela B R Leal
Sepsis is a potentially lethal condition, and it is associated with platelet alterations. The present study sought to investigate the activity of ecto-nucleoside triphosphate diphosphohydrolase (E-NTPDase), E-5'-nucleotidase, and ecto-adenosine deaminase (E-ADA) in the platelets of rats that were induced with sepsis. Male Wistar rats were divided into three groups of ten animals each: a negative control group (normal; NC); a group that underwent surgical procedures (sham); and a group that underwent cecal ligation and perforation (CLP)...
October 2017: Microbial Pathogenesis
Mahnoush Bahjat, Jeroen E J Guikema
Lymphocytes are endowed with unique and specialized enzymatic mutagenic properties that allow them to diversify their antigen receptors, which are crucial sensors for pathogens and mediators of adaptive immunity. During lymphocyte development, the antigen receptors expressed by B and T lymphocytes are assembled in an antigen-independent fashion by ordered variable gene segment recombinations (V(D)J recombination), which is a highly ordered and regulated process that requires the recombination activating gene products 1 &amp; 2 (RAG1, RAG2)...
August 30, 2017: International Journal of Molecular Sciences
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