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TNF alpha and Mesenchymal Stem Cell

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https://www.readbyqxmd.com/read/30107848/neuroprotection-and-immunomodulation-following-intraspinal-axotomy-of-motoneurons-by-treatment-with-adult-mesenchymal-stem-cells
#1
A B Spejo, G B Chiarotto, A D F Ferreira, D A Gomes, R S Ferreira, B Barraviera, A L R Oliveira
BACKGROUND: Treatment of spinal cord injury is dependent on neuronal survival, appropriate synaptic circuit preservation, and inflammatory environment management. In this sense, mesenchymal stem cell (MSC) therapy is a promising tool that can reduce glial reaction and provide trophic factors to lesioned neurons. METHODS: Lewis adult female rats were submitted to a unilateral ventral funiculus cut at the spinal levels L4, L5, and L6. The animals were divided into the following groups: IA (intramedullary axotomy), IA + DMEM (Dulbecco's modified Eagle's medium), IA + FS (fibrin sealant), IA + MSC (106 cells), and IA + FS + MSC (106 cells)...
August 14, 2018: Journal of Neuroinflammation
https://www.readbyqxmd.com/read/30093947/effects-of-human-amnion-derived-mesenchymal-stem-cells-and-conditioned-medium-in-rats-with-sclerosing-cholangitis
#2
Ryo Sugiura, Shunsuke Ohnishi, Masatsugu Ohara, Marin Ishikawa, Shuichi Miyamoto, Reizo Onishi, Koji Yamamoto, Kazumichi Kawakubo, Masaki Kuwatani, Naoya Sakamoto
Mesenchymal stem cells (MSCs) represent a valuable cell source in regenerative medicine, and large numbers of MSCs can be isolated from the amnion noninvasively. Sclerosing cholangitis is a chronic cholestatic disease and characterized by progressive biliary destruction leading to cirrhosis. Many factors are involved in the development of sclerosing cholangitis; however, effective medical therapy is not established. We investigated the effects of human amnion-derived MSCs (hAMSCs) and conditioned medium (CM) obtained from hAMSC cultures in rats with sclerosing cholangitis...
2018: American Journal of Translational Research
https://www.readbyqxmd.com/read/30021657/the-role-of-mesenchymal-stem-cells-in-chemotherapy-induced-gonadotoxicity
#3
Iman O Sherif, Dina Sabry, Azza Abdel-Aziz, Osama M Sarhan
BACKGROUND: The therapeutic potential of bone marrow-derived mesenchymal stem cells (BM-MSCs) against cisplatin-induced nephrotoxicity has been reported, however, its efficacy in gonadotoxicity still has not been addressed. Herein, we investigated the effect of BM-MSCs in cisplatin-induced testicular toxicity and its underlying mechanism of action. METHODS: Thirty male Sprague-Dawley rats were divided into a control group: injected with phosphate-buffered saline (PBS) intraperitoneal (ip), a cisplatin group: injected with a single dose of 7 mg/kg cisplatin ip to induce gonadotoxicity and a BM-MSCs group: received cisplatin ip followed by BM-MSCs injection 1 day after cisplatin...
July 18, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/30003150/suppression-of-t-cells-by-mesenchymal-and-cardiac-progenitor-cells-is-partly-mediated-via-extracellular-vesicles
#4
F van den Akker, K R Vrijsen, J C Deddens, J W Buikema, M Mokry, L W van Laake, P A Doevendans, J P G Sluijter
Adverse remodeling after myocardial infarction (MI) is strongly influenced by T cells. Stem cell therapy after MI, using mesenchymal stem cells (MSC) or cardiomyocyte progenitor cells (CMPC), improved cardiac function, despite low cell retention and limited differentiation. As MSC secrete many factors affecting T cell proliferation and function, we hypothesized the immune response could be affected as one of the targets of stem cell therapy. Therefore, we studied the immunosuppressive properties of human BM-MSC and CMPC and their extracellular vesicles (EVs) in co-culture with activated T cells...
June 2018: Heliyon
https://www.readbyqxmd.com/read/30003044/crohn-s-disease-patient-serum-changes-protein-expression-in-a-human-mesenchymal-stem-cell-model-in-a-linear-relationship-to-patients-disease-stage-and-to-bone-mineral-density
#5
Martina Blaschke, Regine Koepp, Christof Lenz, Jochen Kruppa, Klaus Jung, Heide Siggelkow
Background: Crohn's disease (CD) is associated with a higher prevalence of osteoporosis, a complication that is recognized as a significant cause of morbidity. Its pathogenesis is controversial, but the activity of CD is one contributing factor. Methods: We stimulated SCP-1 cells (mesenchymal stem cell line) under osteogenic conditions with serum from adult patients with CD in the symptomatic phase (SP) and in remission (R) and with control sera. Concentrations of IL-6, IL-1 beta, and TNF alpha in the sera were measured...
September 2018: Journal of Clinical & Translational Endocrinology
https://www.readbyqxmd.com/read/29959311/resveratrol-enhances-the-functionality-and-improves-the-regeneration-of-mesenchymal-stem-cell-aggregates
#6
Yi-Jing Wang, Pan Zhao, Bing-Dong Sui, Nu Liu, Cheng-Hu Hu, Ji Chen, Chen-Xi Zheng, An-Qi Liu, Kun Xuan, Ya-Ping Pan, Yan Jin
Mesenchymal stem cell (MSC)-based regeneration, specifically cell aggregate or cell sheet engineering, is a promising approach for tissue reconstruction. Considering the advantages of ease of harvest and lack of immune rejection, the application of autologous MSCs (i.e., patients' own MSCs) in regenerative medicine has developed considerable interest. However, the impaired cell viability and regenerative potential following MSCs impacted by disease remain a major challenge. Resveratrol (RSV) exhibits reliable and extensive rejuvenative activities that have received increasing clinical attention...
June 27, 2018: Experimental & Molecular Medicine
https://www.readbyqxmd.com/read/29905411/anti-inflammatory-and-antioxidant-effects-of-mesenchymal-and-hematopoietic-stem-cells-in-a-rheumatoid-arthritis-rat-model
#7
Hanan Abdelmawgoud, Asmaa Saleh
BACKGROUND: Mesenchymal stem cells (MSCs) are of increased importance because of their capacity to counteract inflammation and suppress host immune responses. OBJECTIVES: The aim of the study was to compare the effectiveness of MSCs and hematopoietic stem cells (HSCs) in the treatment of rheumatoid arthritis (RA). MATERIAL AND METHODS: Paw swelling was assessed by measuring the thickness of the hind paws using a caliper. Cytokines - interleukin (IL)-6, tumor necrosis factor-alpha (TNF-α), transforming growth factor-beta (TGF-β), and IL-10 - and rheumatoid factor (RF) were measured using enzyme-linked immunosorbent assay (ELISA) kits...
June 15, 2018: Advances in Clinical and Experimental Medicine: Official Organ Wroclaw Medical University
https://www.readbyqxmd.com/read/29890767/effect-of-different-preconditioning-regimens-on-the-expression-profile-of-murine-adipose-derived-stromal-stem-cells
#8
Patrick C Baer, Jürgen M Overath, Anja Urbschat, Ralf Schubert, Benjamin Koch, Asanke A Bohn, Helmut Geiger
Stem cell-based therapies require cells with a maximum regenerative capacity in order to support regeneration after tissue injury and organ failure. Optimization of this regenerative potential of mesenchymal stromal/stem cells (MSC) or their conditioned medium by in vitro preconditioning regimens are considered to be a promising strategy to improve the release of regenerative factors. In the present study, MSC were isolated from inguinal adipose tissue (mASC) from C57BL/6 mice, cultured, and characterized. Then, mASC were either preconditioned by incubation in a hypoxic environment (0...
June 10, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29858072/inflammatory-related-p62-triggers-malignant-transformation-of-mesenchymal-stem-cells-through-the-cascade-of-cudr-ctcf-igfii-ras-signaling
#9
Xiaoru Xin, Chen Wang, Zhuojia Lin, Jie Xu, Yanan Lu, Qiuyu Meng, Xiaonan Li, Yuxin Yang, Qidi Zheng, Xin Gui, Tianming Li, Hu Pu, Wujun Xiong, Jiao Li, Song Jia, Dongdong Lu
Inflammatory and autophagy-related gene P62 is highly expressed in most human tumor tissues. Herein, we demonstrate that P62 promotes human mesenchymal stem cells' malignant transformation via the cascade of P62-tumor necrosis factor alpha (TNF-α)-CUDR-CTCF-insulin growth factor II (IGFII)-H-Ras signaling. Mechanistically, we reveal P62 enhances IGFII transcriptional activity through forming IGFII promoter-enhancer chromatin loop and increasing METTL3 occupancy on IGFII 3' UTR and enhances H-Ras overexpression by harboring inflammation-related factors, e...
June 1, 2018: Molecular Therapy. Nucleic Acids
https://www.readbyqxmd.com/read/29852392/total-glucosides-of-paeony-improves-the-immunomodulatory-capacity-of-mscs-partially-via-the-mir-124-stat3-pathway-in-oral-lichen-planus
#10
Zhongfang Zhao, Ying Han, Zhihui Zhang, Wenwen Li, Xiaoli Ji, Xiaodan Liu, Jianqiu Jin, Si Xu, Hongmei Cui, Zhe Cheng, Qian Wang, Xing Wang, Xiang Guo, Yixiang Wang, Hongwei Liu
Mesenchymal stem cells (MSCs) have been used clinically and experimentally to relieve severe immune-related diseases due to their immunomodulatory properties, but these are impaired by inflammation. Oral lichen planus (OLP) is a T cell-mediated chronic inflammatory mucosal disease. In the present study, we found MSCs from OLP with higher expression of interleukin (IL)-6, tumour necrosis factor alpha (TNF-α), transforming growth factor beta (TGF-β) and IL-10 compared with control. Total glucosides of paeony (TGP) significantly improves the immunomodulatory function of MSCs by inhibiting IL-6 and TNF-α expression and increasing TGF-β and IL-10 expression...
September 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29790427/simulated-microgravity-culture-enhances-the-neuroprotective-effects-of-human-cranial-bone-derived-mesenchymal-stem-cells-in-traumatic-brain-injury
#11
Takashi Otsuka, Takeshi Imura, Kei Nakagawa, Looniva Shrestha, Shinya Takahashi, Yumi Kawahara, Taijiro Sueda, Kaoru Kurisu, Louis Yuge
Fundamental cures of central nervous system (CNS) diseases are rarely achieved due to the low regenerative ability of the CNS. Recently, cell-based therapy using mesenchymal stem cells (MSCs) has been explored as an effective treatment for CNS diseases. Among the various tissue-derived MSCs, we have isolated human cranial bone-derived MSCs (cMSCs) in our laboratory. In addition, we have focused on simulated microgravity (MG) as a valuable culture environment of MSCs. However, detailed mechanisms underlying functional recovery from transplantation of MSCs cultured under MG conditions remain unclear...
July 5, 2018: Stem Cells and Development
https://www.readbyqxmd.com/read/29552635/isolation-and-identification-of-proteins-secreted-by-cells-cultured-within-synthetic-hydrogel-based-matrices
#12
Lisa A Sawicki, Leila H Choe, Katherine L Wiley, Kelvin H Lee, April M Kloxin
Cells interact with and remodel their microenvironment, degrading large extracellular matrix (ECM) proteins (e.g., fibronectin, collagens) and secreting new ECM proteins and small soluble factors (e.g., growth factors, cytokines). Synthetic mimics of the ECM have been developed as controlled cell culture platforms for use in both fundamental and applied studies. However, how cells broadly remodel these initially well-defined matrices remains poorly understood and difficult to probe. In this work, we have established methods for widely examining both large and small proteins that are secreted by cells within synthetic matrices...
March 12, 2018: ACS Biomaterials Science & Engineering
https://www.readbyqxmd.com/read/29536646/testing-the-potency-of-anti-tnf-%C3%AE-and-anti-il-1%C3%AE-drugs-using-spheroid-cultures-of-human-osteoarthritic-chondrocytes-and-donor-matched-chondrogenically-differentiated-mesenchymal-stem-cells
#13
Sara Žigon-Branc, Ariana Barlič, Miomir Knežević, Matjaž Jeras, Gordana Vunjak-Novakovic
Inflammation plays a major role in progression of rheumatoid arthritis, a disease treated with antagonists of tumor necrosis factor-alpha (TNF-α) and interleukin 1β (IL-1β). New in vitro testing systems are needed to evaluate efficacies of new anti-inflammatory biological drugs, ideally in a patient-specific manner. To address this need, we studied microspheroids containing 10,000 human osteoarthritic primary chondrocytes (OACs) or chondrogenically differentiated mesenchymal stem cells (MSCs), obtained from three donors...
March 14, 2018: Biotechnology Progress
https://www.readbyqxmd.com/read/29463990/mesenchymal-stromal-stem-cell-derived-extracellular-vesicles-promote-human-cartilage-regeneration-in-vitro
#14
Lucienne A Vonk, Sanne F J van Dooremalen, Nalan Liv, Judith Klumperman, Paul J Coffer, Daniël B F Saris, Magdalena J Lorenowicz
Osteoarthritis (OA) is a rheumatic disease leading to chronic pain and disability with no effective treatment available. Recently, allogeneic human mesenchymal stromal/stem cells (MSC) entered clinical trials as a novel therapy for OA. Increasing evidence suggests that therapeutic efficacy of MSC depends on paracrine signalling. Here we investigated the role of extracellular vesicles (EVs) secreted by human bone marrow derived MSC (BMMSC) in human OA cartilage repair. METHODS: To test the effect of BMMSC-EVs on OA cartilage inflammation, TNF-alpha-stimulated OA chondrocyte monolayer cultures were treated with BMMSC-EVs and pro-inflammatory gene expression was measured by qRT-PCR after 48 h...
2018: Theranostics
https://www.readbyqxmd.com/read/29435145/tnf-%C3%AE-inhibits-satb2-expression-and-osteoblast-differentiation-through-nf-%C3%AE%C2%BAb-and-mapk-pathways
#15
Chijian Zuo, Xiaoying Zhao, Yu Shi, Wen Wu, Ning Zhang, Jiake Xu, Chuandong Wang, Guoli Hu, Xiaoling Zhang
Although the mechanisms of Tumor necrosis factor alpha (TNF-α) on facilitating osteoclast differentiation and bone resorption is well known, the mechanisms behind the suppression of the osteoblast differentiation from mesenchymal stem cells (MSCs) are still poorly understood. In this study, we observed a negative correlation between TNF-α levels and the expression of special AT-rich sequence-binding protein 2 (SATB2), a critical osteoblastogenesis transcription factor, in ovariectomy (OVX)-induced bone loss and IL-1-induced arthritis animal model...
January 12, 2018: Oncotarget
https://www.readbyqxmd.com/read/29359591/bone-marrow-mesenchymal-stem-cells-suppress-il-9-in-adjuvant-induced-arthritis
#16
Sahar Sobhy Abd Elhalem, Nawal Zakaria Haggag, Nashwa Ahmed El-Shinnawy
Interleukin-9 (IL-9) has been shown to be upregulated in rheumatoid arthritis (RA). The exact role of IL-9 has not yet been effectively studied. Mesenchymal stem cells (MSCs) have shown a promising immunomodulatory role towards repairing cartilage and restoring joint function. One of the key problems influencing the therapeutic efficacy of stem cell therapy is the poor cell survival following transplantation. This is attributed to oxidative and inflammatory stresses at the injured sites. Hesperidin (Hsd), a flavanone present in citrus fruits, has been studied as potential therapeutic agents that have anti-oxidant and anti-inflammatory activities...
February 2018: Autoimmunity
https://www.readbyqxmd.com/read/29301108/mesenchymal-stromal-cells-inhibit-inflammatory-lymphangiogenesis-in-the-cornea-by-suppressing-macrophage-in-a-tsg-6-dependent-manner
#17
Hyun Beom Song, Se Yeon Park, Jung Hwa Ko, Jong Woo Park, Chang Ho Yoon, Dong Hyun Kim, Jeong Hun Kim, Mee Kum Kim, Ryang Hwa Lee, Darwin J Prockop, Joo Youn Oh
The cornea is a transparent tissue devoid of blood and lymphatic vessels. However, various inflammatory conditions can cause hemangiogenesis and lymphangiogenesis in the cornea, compromising transparency and visual acuity. Mesenchymal stem/stromal cells (MSCs) have therapeutic potentials in a variety of diseases because of anti-inflammatory properties. Herein, we investigated the effects of MSCs on corneal angiogenesis using a model of suture-induced inflammatory corneal neovascularization. Data demonstrated that an intravenous administration of MSCs suppressed corneal inflammation and neovascularization, inhibiting both hemangiogenesis and lymphangiogenesis...
January 3, 2018: Molecular Therapy: the Journal of the American Society of Gene Therapy
https://www.readbyqxmd.com/read/29259700/neuroprotective-effects-of-human-umbilical-cord-derived-mesenchymal-stem-cells-on-periventricular-leukomalacia-like-brain-injury-in-neonatal-rats
#18
Chikako Morioka, Motohiro Komaki, Atsuko Taki, Izumi Honda, Naoki Yokoyama, Kengo Iwasaki, Sachiko Iseki, Tomohiro Morio, Ikuo Morita
Background: Periventricular leukomalacia (PVL) is a type of multifactorial brain injury that causes cerebral palsy in premature infants. To date, effective therapies for PVL have not been available. In this study, we examined whether mesenchymal stem cells (MSCs) possess neuroprotective property in a lipopolysaccharide (LPS)-induced neonatal rat PVL-like brain injury. Methods: Human umbilical cord-derived MSCs (UCMSCs) were used in this study. Four-day-old rats were intraperitoneally injected with LPS (15 mg/kg) to cause the PVL-like brain injury and were treated immediately after the LPS-injection with UCMSCs, conditioned medium prepared from MSCs (UCMSC-CM) or interferon-gamma (IFN-γ)-pretreated MSC (IFN-γ-UCMSC-CM)...
2017: Inflammation and Regeneration
https://www.readbyqxmd.com/read/29259373/mesenchymal-stem-cells-rescue-acute-hepatic-failure-by-polarizing-m2-macrophages
#19
Yan-Wei Li, Chong Zhang, Qiu-Ju Sheng, Han Bai, Yang Ding, Xiao-Guang Dou
AIM: To investigate whether M1 or M2 polarization contributes to the therapeutic effects of mesenchymal stem cells (MSCs) in acute hepatic failure (AHF). METHODS: MSCs were transfused into rats with AHF induced by D-galactosamine (DGalN). The therapeutic effects of MSCs were evaluated based on survival rate and hepatocyte proliferation and apoptosis. Hepatocyte regeneration capacity was evaluated by the expression of the hepatic progenitor surface marker epithelial cell adhesion molecule (EpCAM)...
December 7, 2017: World Journal of Gastroenterology: WJG
https://www.readbyqxmd.com/read/29249319/innate-immune-responses-of-equine-monocytes-cultured-in-equine-platelet-lysate
#20
Maria C Naskou, Natalie A Norton, Ian B Copland, Jacques Galipeau, John F Peroni
Platelet lysate (PL) has been extensively used for the laboratory expansion of human mesenchymal stem cells (MSC) in order to avoid fetal bovine serum (FBS) which has been associated with immune-mediated host reactions and transmission of bovine-origin microbial contaminants. Before suggesting the routine use of PL for MSC culture, we wanted to further investigate whether PL alone might trigger inflammatory responses when exposed to reactive white blood cells such as monocytes. Our objectives were to evaluate the inflammatory profile of equine monocytes cultured with equine PL (ePL) and to determine if ePL can modulate the expression of inflammatory cytokines in lipopolysaccharide (LPS)-stimulated monocytes...
January 2018: Veterinary Immunology and Immunopathology
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