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https://www.readbyqxmd.com/read/29909459/electrochemical-nonenzymatic-sensor-for-cholesterol-determination-in-food
#1
Ksenia Derina, Elena Korotkova, Yekaterina Taishibekova, Lyazat Salkeeva, Bohumil Kratochvil, Jiri Barek
The treatment of some inborn metabolism errors requires cholesterol substitution therapy. Cholesterol plays a vital role in the human body. Therefore, the majority of cholesterol determination techniques are targeted to blood and blood serum. Nevertheless, cholesterol determination in food is important as well. In this paper, cholesterol determination using differential pulse voltammetry (DPV) in dairy products (e.g., milk, clotted cream, yogurt, butter, etc.) is reported with a novel nonenzymatic sensor based on diphosphonic acid of 1,4-diacetylglycoluril (DPADGU) as an electrode surface modifier...
June 16, 2018: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/29908638/gadd45a-knockdown-alleviates-oxidative-stress-through-suppressing-the-p38-mapk-signaling-pathway-in-the-pathogenesis-of-preeclampsia
#2
Fa-Hong Li, Na Han, Yong Wang, Qian Xu
OBJECTIVE: Preeclampsia (PE) is a hypertensive disease associated with vascular oxidative stress (OS). Besides, cell response to OS triggers growth arrest and DNA damage inducible alpha (Gadd45a). Herein, we investigated the effect of Gadd45a on OS in PE. METHODS: Umbilical cord tissues and peripheral blood samples were collected from PE patients and normal pregnant women. Immunohistochemistry was applied to identify Gadd45a level and extent of p38 phosphorylation...
May 2018: Placenta
https://www.readbyqxmd.com/read/29908544/scrutinizing-the-expression-and-blockade-of-inhibitory-molecules-expressed-on-t-cells-from-acute-myeloid-leukemia-patients
#3
Mohsen Abdolmaleki, Nazanin Mojtabavi, Mahdi Zavvar, Mohammad Vaezi, Farshid Noorbakhsh, Mohammad Hossein Nicknam
T cell exhaustion is an immunosuppressive mechanism which occurs in chronic viral infections, solid tumors and hematologic malignancies. Exhausted T cell has increased the expression of inhibitory receptors, and functional impairment. In this study, we investigated the expression from some of those inhibitory receptors being Programmed death 1 (PD-1), T cell immunoglobulin and mucin domain containing molecules 3 (TIM-3) and CD244 on T cells from Iranian acute myeloid leukemia (AML) patients. Peripheral blood samples were collected from Iranian newly diagnosed AML patients and flow cytometric analysis was accomplished for cell surface expression of PD-1, TIM-3, and CD244 on T lymphocytes...
June 2018: Iranian Journal of Allergy, Asthma, and Immunology
https://www.readbyqxmd.com/read/29908455/oxidative-damage-and-antioxidant-defense-in-caiman-latirostris-broad-snouted-caiman-exposed-in-ovo-to-pesticide-formulations
#4
P M Burella, L M Odetti, M F Simoniello, G L Poletta
The surface used for agricultural production in Argentina significantly increased in recent years, mainly due to the expansion of soybean crops. As a result, the use of agrochemicals increased too. Many natural populations of Caiman latirostris (broad-snouted caiman) are affected by habitat fragmentation and the constant exposure to pesticides. This exposure could produce Reactive Oxygen Species. The negative imbalance between ROS generation and the capacity of the biological systems to eliminate the reactive intermediaries or avoid the damage is called Oxidative Stress...
June 13, 2018: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/29908347/aging-related-functional-and-structural-changes-in-the-heart-and-aorta-mitotempo-improves-aged-cardiovascular-performance
#5
Yusuf Olgar, Sinan Degirmenci, Aysegul Durak, Deniz Billur, Belgin Can, Gizem Kayki Mutlu, Ebru Arioglu Inan, Belma Turan
Aging in humans represents declining in cardio-protective systems, however its mechanisms are not known yet. We aimed to analyse how aging affects key mechanisms responsible for contractile dysfunction via comparing the improperly synchrony between electrical and mechanical activities in male aged-rats (24-month old) comparison to those of adult-rats (6-month old). We determined significantly increased systemic oxidative stress with decreased antioxidant capacity, clear insulin resistance and hypertrophy in aged-rats with normal fasting blood glucose...
June 13, 2018: Experimental Gerontology
https://www.readbyqxmd.com/read/29908163/matrix-metalloproteinases-are-regulated-by-microrna-320-in-macrophages-and-are-associated-with-aortic-dissection
#6
Mingfang Liao, Sili Zou, Yan Bao, Jie Jin, Junlin Yang, Yandong Liu, Mark Green, Futang Yang, Lefeng Qu
Aortic dissection (AD) is the circumferential or transversal tear of the aorta wall that allows blood to infiltrate the layers. MicroRNA (miR) analyses have demonstrated a correlation between miR-320 family and AD. The underlying mechanism is yet unclear. The matrix metalloproteinases (MMPs) are a group of proteolytic enzymes that could catalyze the degeneration of the extracellular matrix and the destruction of the vasculature. In this study, we investigated whether miR-320 presented a role in regulating the production of MMPs in aortic dissection...
June 13, 2018: Experimental Cell Research
https://www.readbyqxmd.com/read/29908151/vaccination-with-a-cd4-and-cd8-t-cell-epitopes-based-recombinant-chimeric-protein-derived-from-leishmania-infantum-proteins-confers-protective-immunity-against-visceral-leishmaniasis
#7
Daniel S Dias, Patrícia A F Ribeiro, Vívian T Martins, Daniela P Lage, Lourena E Costa, Miguel A Chávez-Fumagalli, Fernanda F Ramos, Thaís T O Santos, Fernanda Ludolf, Jamil S Oliveira, Tiago A O Mendes, Eduardo S Silva, Alexsandro S Galdino, Mariana C Duarte, Bruno M Roatt, Daniel Menezes-Souza, Antonio L Teixeira, Eduardo A F Coelho
Vaccination seems to be the best approach to control visceral leishmaniasis (VL). Resistance against infection is based on the development of a Th1 immune response characterized by the production of interferons-γ (IFN-γ), interleukin-12 (IL-12), granulocyte-macrophage-colony-stimulating factor (GM-CSF), and tumor necrosis factor-α (TNF-α), among others. A number of antigens have been tested as potential targets against the disease; few of them are able to stimulate human immune cells. In the present study, 1 prediction of MHC class I and II molecules-specific epitopes in the amino acid sequences of 3 Leishmania proteins: 1 hypothetical, prohibitin, and small glutamine-rich tetratricopeptide repeat-containing proteins, was performed using bioinformatics tools, and a T-cell epitopes-based recombinant chimeric protein was constructed, synthetized and purified to be evaluated in invitro and in vivo experiments...
May 22, 2018: Translational Research: the Journal of Laboratory and Clinical Medicine
https://www.readbyqxmd.com/read/29908067/effects-of-type-i-interferons-in-malaria
#8
REVIEW
Ismail Sebina, Ashraful Haque
Type I interferons are a family of cytokines with a wide range of biological activities including anti-viral and immune-regulatory functions. Here, we focus on the protozoan parasitic disease, malaria, and examine the effects of type I IFN-signalling during Plasmodium infection of humans and experimental mice. Since the 1960's, there have been many studies in this area, but a simple explanation for the role of type I IFN has not emerged. Although epidemiological data are consistent with roles for type I IFN in influencing malaria disease severity, functional proof of this remains sparse in humans...
June 16, 2018: Immunology
https://www.readbyqxmd.com/read/29908029/fc%C3%AE-ri-fc%C3%AE-rii-and-il-10-as-predictive-biomarkers-for-post-therapeutic-cicatrization-time-in-monocytes-from-cutaneous-leishmaniasis-patients
#9
Marina L Rodrigues-Alves, Otoni A O Melo-Júnior, Jordana G Coelho-Dos-Reis, Marcelo A Pascoal-Xavier, Heloisa Alves-Costa, Clovis A Reis, Walderez O Dutra, Rosiana E Silva, Maria C R Senna, Andreia C Faria, Nayara I Medeiros, Juliana A S Gomes, Denise Silveira-Lemos, Olindo A Martins-Filho, Andréa Teixeira-Carvalho, Matheus F Costa-Silva, Rodolfo C Giunchetti, Vanessa Peruhype-Magalhães
Cutaneous Leishmaniasis (CL) treatment is based on therapy with Glucantime® , yet there are few laboratory methods to monitor its success. In this study, ex vivo and in vitro evaluations of peripheral blood monocytes were performed in a longitudinal study to characterize the impact of Glucantime® on overall phenotypic/functional features of these cells from CL patients to identify predictive biomarkers for post-therapeutic monitoring by flow cytometry. The ex vivo evaluation from CL patients demonstrated a modulatory profile before treatment, with a decrease in TLR-2, FcγRII, HLA-DR, CD86, IFN-γR, TNF, IL-12, NO, and an increase in FcγRIII and IL-10R...
June 16, 2018: Parasite Immunology
https://www.readbyqxmd.com/read/29908017/the-role-of-access-to-affordable-and-quality-assured-blood-and-blood-products-for-achieving-universal-health-coverage
#10
EDITORIAL
Yetmgeta Abdella, Ali Akbar Pourfathollah, Hmida Slama, May Raouf
No abstract text is available yet for this article.
June 10, 2018: Eastern Mediterranean Health Journal, la Revue de Santé de la Méditerranée Orientale
https://www.readbyqxmd.com/read/29907766/efficient-rna-drug-delivery-using-red-blood-cell-extracellular-vesicles
#11
Waqas Muhammad Usman, Tin Chanh Pham, Yuk Yan Kwok, Luyen Tien Vu, Victor Ma, Boya Peng, Yuen San Chan, Likun Wei, Siew Mei Chin, Ajijur Azad, Alex Bai-Liang He, Anskar Y H Leung, Mengsu Yang, Ng Shyh-Chang, William C Cho, Jiahai Shi, Minh T N Le
Most of the current methods for programmable RNA drug therapies are unsuitable for the clinic due to low uptake efficiency and high cytotoxicity. Extracellular vesicles (EVs) could solve these problems because they represent a natural mode of intercellular communication. However, current cellular sources for EV production are limited in availability and safety in terms of horizontal gene transfer. One potentially ideal source could be human red blood cells (RBCs). Group O-RBCs can be used as universal donors for large-scale EV production since they are readily available in blood banks and they are devoid of DNA...
June 15, 2018: Nature Communications
https://www.readbyqxmd.com/read/29907206/rotational-thromboelastometry-thresholds-for-patients-at-risk-for-massive-transfusion
#12
Gregory R Stettler, Ernest E Moore, Geoffrey R Nunns, Jim Chandler, Erik Peltz, Christopher C Silliman, Anirban Banerjee, Angela Sauaia
BACKGROUND: Goal-directed hemostatic resuscitation based on thrombelastography has a survival benefit compared to conventional coagulation assays. While thrombelastography transfusion thresholds for patients at risk for massive transfusion (MT) have been defined, similar cutoffs do not exist for the other commonly used viscoelastic assay, rotational thromboelastometry (ROTEM). The purpose of this study was to develop ROTEM blood product thresholds in patients at risk for MT. METHODS: ROTEM was assessed in trauma activation patients admitted from 2010 to 2016 (n = 222)...
August 2018: Journal of Surgical Research
https://www.readbyqxmd.com/read/29907111/changes-in-biomarkers-in-equine-synovial-fluid-two-weeks-after-intra-articular-hyaluronan-treatment-a-randomised-double-blind-clinical-trial
#13
Tytti M Niemelä, Riitta-Mari Tulamo, Kaisa Aaltonen, Satu M Sankari, Anna K Hielm-Björkman
BACKGROUND: Inflammatory and degenerative activity inside the joint can be studied in vivo via analysis of synovial fluid (SF) biomarkers, which are molecular markers of inflammatory processes and tissue turnover. The aim of this study was to investigate the response of selected biomarkers in the SF after an intra-articular (IA) high-molecular-weight non-animal stabilized hyaluronic acid (NASHA) treatment. Our hypothesis was that prostaglandin E2 (PGE2 ), substance P, aggrecan chondroitin sulfate 846 epitope (CS846), and carboxypeptide of type II collagen (CPII) concentrations in SF would decrease more in the NASHA than in the placebo group...
June 15, 2018: BMC Veterinary Research
https://www.readbyqxmd.com/read/29907093/effect-of-administration-of-a-probiotic-preparation-on-gut-microbiota-and-immune-response-in-healthy-women-in-india-an-open-label-single-arm-pilot-study
#14
Ankita Singh, Aditya N Sarangi, Amit Goel, Rajni Srivastava, Rajat Bhargava, Priyanka Gaur, Amita Aggarwal, Rakesh Aggarwal
BACKGROUND: Probiotics have been shown to be useful for the treatment of many disease conditions. These beneficial effects are believed to be mediated by change in the composition of gut microbiota and modulation of the host immune responses. However, the available data on the effect of probiotics on these parameters are quite limited. METHODS: We studied the composition of fecal microbiota, using 16S rRNA sequencing, and host immune responses in peripheral blood (plasma cytokine levels, T cell subsets and in vitro cytokine production after stimulation with anti-CD3/CD28 antibody or lipopolysaccharide) in a group of 14 healthy women at three time-points - before and after administration of a probiotic preparation (a capsule of VSL#3, each containing 112...
June 15, 2018: BMC Gastroenterology
https://www.readbyqxmd.com/read/29906662/hormonal-stimulation-in-4-to-7-months-old-nelore-bos-taurus-indicus-females-improved-ovarian-follicular-responses-but-not-the-in-vitro-embryo-production
#15
Thaís Abritta Zacarias, Taynan Stonoga Kawamoto, João Ricardo Scaliante Júnior, Ana Luiza Silva Guimarães, Maurício Machaim Franco, Ricardo Alamino Figueiredo
The inclusion of pre-pubertal bovine females in reproductive management could allow in vitro embryo production and reduce generation interval, thereby causing faster genetic gain of the herd. However, oocytes of pre-pubertal females have lower competence, blastocyst production, and pregnancy rates than those collected from pubertal animals. This study aimed to evaluate the effect of an induced hormonal stimulation on the serum concentrations of Anti-Mullerian hormone (AMH) and FSH, ovarian responses, ovum pick up (OPU), and in vitro produced embryos (IVP) from oocytes obtained from four-to seven-months old Nelore female cattle...
May 31, 2018: Theriogenology
https://www.readbyqxmd.com/read/29906528/cd300c-co-stimulates-ige-mediated-basophils-activation-and-its-expression-is-increased-in-cow%C3%A2-s-milk-allergy
#16
Olatz Zenarruzabeitia, Joana Vitallé, Iñigo Terrén, Ane Orrantia, Itziar Astigarraga, Leire Dopazo, Carlos Gonzalez, Laura Santos-Díez, Carlos Tutau, Pedro M Gamboa, Agurtzane Bilbao, Francisco Borrego
BACKGROUND: Basophils express high-affinity IgE receptors (FcεRI), which play an essential role in allergic diseases. To design promising immunomodulatory therapies, it is important to characterize new cell surface receptors that modulate immunoglobulin (Ig)E-mediated basophil activation threshold. OBJECTIVES: To analyze the expression of CD300 receptors on human basophils and their implication in IgE-mediated basophil activation processes. METHODS: Blood samples from healthy and cow´s milk allergic patients were collected through the Basque Biobank under an institutional review board-approved protocol...
June 12, 2018: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/29906471/self-selection-of-plant-bioactive-compounds-by-sheep-in-response-to-challenge-infection-with-haemonchus-contortus
#17
Cesar H E C Poli, Kara J Thornton-Kurth, Jerrad F Legako, Carolina Bremm, Viviane S Hampel, Jeffery Hall, Ignacio R Ipharraguerre, Juan J Villalba
Plant bioactives can potentially benefit herbivores through their effects on health and nutrition. The objective of this study was to determine the importance of polyphenols and terpenes on the ability of lambs to self-select these compounds when challenged by a parasitic infection and the subsequent impact on their health and productivity. Thirty-five lambs were housed in individual pens and assigned to five treatment groups (7 animals/group), where they received: 1) A basal diet of beet pulp:soybean meal (90:10) (CONTROL); 2) The same diet, but containing 0...
June 12, 2018: Physiology & Behavior
https://www.readbyqxmd.com/read/29906406/the-malignancy-index-in-plasma-samples-as-a-prostate-cancer-biomarker
#18
Antonio M Serafin, Angela Chinhengo, Pedro Fernandez, John M Akudugu
No unambiguous role of the involvement of uroplasminogen activator (uPA) and plasminogen activator inhibitor type-1 (PAI-1) in prostate cancer has emerged, with current evidence suggesting that neither biomarker is likely of significant clinical value, save as an overall contributor. In this study, we attempt to discriminate prostate cancer from non-cancer in a cohort of plasma samples, using the Imubind ELISA assay. In this cohort, PAI-1 levels are higher in prostate cancer patients than healthy donors; uPA levels are higher in healthy donors than prostate cancer patients; and the uPA/PAI-1 ratio is higher in healthy donors than in prostate cancer patients...
May 2018: Discovery Medicine
https://www.readbyqxmd.com/read/29905672/alterations-in-mitochondrial-function-in-blood-cells-obtained-from-patients-with-sepsis-presenting-to-an-emergency-department
#19
David H Jang, Clinton J Orloski, Shawn Owiredu, Frances S Shofer, John C Greenwood, David M Eckmann
OBJECTIVE: Mitochondrial dysfunction has been implicated as a key cellular event leading to organ dysfunction in sepsis. Our objective is to measure changes in mitochondrial bioenergetics in subjects with early presentation of sepsis to provide insight into the incompletely understood pathophysiology of the dysregulated host response in sepsis. DESIGN: Prospective observational study SETTING:: Single site tertiary academic emergency department SUBJECTS:: We enrolled a total of 48 subjects were enrolled in the study, 10 with sepsis or septic shock, 10 with infection without sepsis, 14 older and 14 younger healthy controls...
June 13, 2018: Shock
https://www.readbyqxmd.com/read/29904777/science-behind-the-cardio-metabolic-benefits-of-omega-3-polyunsaturated-fatty-acids-biochemical-effects-vs-clinical-outcomes
#20
REVIEW
Rohith N Thota, Jessica J A Ferguson, Kylie A Abbott, Cintia B Dias, Manohar L Garg
Lower incidence of cardiovascular disease (CVD) in the Greenland Inuit, Northern Canada and Japan has been attributed to their consumption of seafood rich in long chain omega-3 polyunsaturated fatty acids (LCn-3PUFA). While a large majority of pre-clinical and intervention trials have demonstrated heart health benefits of LCn-3PUFA, some studies have shown no effects or even negative effects. LCn-3PUFA have been shown to favourably modulate blood lipid levels, particularly a reduction in circulating levels of triglycerides...
June 15, 2018: Food & Function
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