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https://www.readbyqxmd.com/read/29679839/xenon-detection-in-human-blood-analytical-validation-by-accuracy-profile-and-identification-of-critical-storage-parameters
#1
Cécile Frampas, Julia Ney, Mark Coburn, Marc Augsburger, Vincent Varlet
Xenon is a rare, mostly inert, noble gas that has applications in a wide range of fields, including medicine. Xenon acts on the human body as a useful organ-protective and anesthetic agent and has also been previously studied for potential applications in fields such as optics, aerospace and medical imaging. Recently, it was discovered that xenon can boost erythropoietin production, and it has been used as a performance-enhancing agent in international sports competitions such as the Sochi Olympic Games. Therefore, screening methods to detect the misuse of xenon by analysis of biological samples and to monitor anesthesia kinetics and efficiency are being investigated...
April 13, 2018: Journal of Forensic and Legal Medicine
https://www.readbyqxmd.com/read/29679202/tick-infestation-and-occurrence-of-anaplasma-phagocytophilum-and-piroplasms-in-cattle-in-the-republic-of-serbia
#2
Ana Vasić, Marion Nieder, Nemanja Zdravković, Jovan Bojkovski, Dejan Bugarski, Ivan Pavlović, Cornelia Silaghi
Ticks transmit important pathogens affecting cattle such as intracellular bacteria of the genus Anaplasma or protozoa of the order Piroplasmida. This study aimed at assessing tick species present on pastures and cattle and determining occurrence of the tick-borne pathogens Anaplasma phagocytophilum and Babesia spp. in cattle. During May and June 2013, ticks and EDTA blood were collected from 185 dairy cows at six locations in Serbia. Ticks were also collected directly from the pastures at four of these locations...
April 20, 2018: Parasitology Research
https://www.readbyqxmd.com/read/29678850/cryopreservation-of-genotyped-abo-subgroup-rbcs-for-quality-assurance-of-abo-grouping-reagents
#3
Sinyoung Kim, Sungwook Song, Hyun Ok Kim
BACKGROUND: Quality assurance of newly developed or manufactured blood grouping reagents with reagent red blood cells (RBCs) is crucial in the process of product approval by governmental agency. However, RBCs with rare blood group are not easily available in the fresh state. We investigated the feasibility of cryopreserved and genotyped ABO subgroup RBC reagents for quality assurance purpose. METHODS: We obtained RBCs from 10 volunteers with ABO subgroup phenotypes...
March 2018: Annals of Clinical and Laboratory Science
https://www.readbyqxmd.com/read/29678738/autochthonous-lactic-acid-bacteria-isolated-from-pig-faeces-in-thailand-show-probiotic-properties-and-antibacterial-activity-against-enteric-pathogenic-bacteria
#4
Wandee Sirichokchatchawan, Puwiya Pupa, Prasert Praechansri, Nutthee Am-In, Somboon Tanasupawat, Piengchan Sonthayanon, Nuvee Prapasarakul
Lactic acid bacteria (LAB) play an important role in pig health and performance that arises from their beneficial impacts on the balance of gastrointestinal microbes, ability to fight enteric pathogens, and capacity to support the immune system. The aim of this study was to evaluate the functional and safety aspects of five previously isolated autochthonous LAB strains, (Lactobacillus plantarum 22F, 25F and 31F, Pediococcus acidilactici 72N and Pediococcus pentosaceus 77F) from pig faeces as potential probiotics for a pig feed supplement...
April 17, 2018: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29678737/a-novel-purification-procedure-for-recombinant-human-serum-albumin-expressed-in-pichia-pastoris
#5
Shijie Li, Linlin Li, Zhuo Chen, Guangpu Xue, Longguang Jiang, Ke Zheng, Jincan Chen, Rui Li, Cai Yuan, Mingdong Huang
Plasma-derived human serum albumin (pHSA) has important applications in many clinical indications, including blood loss, serious burn, or hemorrhagic shock. The limited supply and potential infectious pathogen contamination of pHSA have stimulated the development of recombinant human serum albumin (rHSA). However, rHSA often entraps endogenous or exogenous impurities, including color pigments and polysaccharides. Therefore, the purification of rHSA to high purity remains the bottleneck in large-scale production of rHSA...
April 17, 2018: Protein Expression and Purification
https://www.readbyqxmd.com/read/29678503/caffeine-inhibits-stat1-signaling-and-downregulates-inflammatory-pathways-involved-in-autoimmunity
#6
Merve Iris, Pei-Suen Tsou, Amr H Sawalha
Caffeine is a widely consumed pharmacologically active product. We focused on characterizing immunomodulatory effects of caffeine on peripheral blood mononuclear cells. Caffeine at high doses showed a robust downregulatory effect on cytokine activity and genes related to several autoimmune diseases including lupus and rheumatoid arthritis. Dose-dependent validation experiments showed downregulation at the mRNA levels of key inflammatory genes including STAT1, TNF, and PPARG. TNF and PPARG were suppressed even with the lowest caffeine dose tested, which corresponds to the serum concentration of caffeine after administration of one cup of coffee...
April 17, 2018: Clinical Immunology: the Official Journal of the Clinical Immunology Society
https://www.readbyqxmd.com/read/29678458/immunization-of-balb-c-mice-with-a-combination-of-four-recombinant-brucella-abortus-proteins-aspc-dps-inpb-and-ndk-confers-a-marked-protection-against-a-virulent-strain-of-brucella-abortus
#7
Huynh Tan Hop, Lauren Togonon Arayan, Tran Xuan Ngoc Huy, Alisha Wehdnesday Bernardo Reyes, WonGi Min, Hu Jang Lee, Soo Jong Park, Hong Hee Chang, Suk Kim
In this study, we assessed the protective efficacy of single subunit vaccines, encoded by the B. abortus 544 genes aspC, dps, yaeC and inpB, against B. abortus infection in mice. First, immunization with these antigens, with the exception of the YaeC protein, was found to elicit both humoral and cellular immune responses with IgG2a being dominant over IgG1. In addition, a massive production of IFN-γ but lower degree of IL-10 was observed, suggesting that all three antigens were able to induce predominantly cell-mediated immunity in response to B...
April 17, 2018: Vaccine
https://www.readbyqxmd.com/read/29678144/interferon-alpha-as-antiviral-therapy-in-chronic-active-epstein-barr-virus-disease-with-interstitial-pneumonia-case-report
#8
Jacek Roliński, Ewelina Grywalska, Aleksandra Pyzik, Michał Dzik, Violetta Opoka-Winiarska, Agata Surdacka, Maciej Maj, Franciszek Burdan, Michał Pirożyński, Piotr Grabarczyk, Elżbieta Starosławska
BACKGROUND: Chronic active Epstein-Barr virus (EBV) disease (CAEBV) is defined as a severe, progressive lymphoproliferative disorder associated with active EBV infection persisting longer than 6 months and developing in patients without recognised immunodeficiency. Rarely, interstitial pneumonitis (IP) occurs as a serious complication in CAEBV patients. The standard therapeutic regimen for IP in CAEBV has not yet been defined. Although interferon alpha (IFN-alpha) is known to suppress viral DNA replication by affecting its basal promoter activation process, it is rarely used in CAEBV patients...
April 20, 2018: BMC Infectious Diseases
https://www.readbyqxmd.com/read/29677833/convenient-colorimetric-detection-of-cholesterol-using-multi-enzyme-co-incorporated-organic-inorganic-hybrid-nanoflowers
#9
Minsoo Chung, Yoon Jung Jang, Moon Il Kim
We have developed a microscale well-plate colorimetric assay for the multiplexed detection of cholesterol in clinical human blood samples. This system utilizes a novel multi-enzyme incorporated organic-inorganic hybrid nanoflower which entrap both cholesterol oxidase (ChOx) and horseradish peroxidase (HRP) as the organic components with copper phosphate as the inorganic component to detect cholesterol levels in blood samples. The hybrid nanoflowers, synthesized via an extremely simple but rapid sonication-mediated method within 5 min at room temperature, enable an efficient one-pot two-enzyme cascade reaction...
September 1, 2018: Journal of Nanoscience and Nanotechnology
https://www.readbyqxmd.com/read/29677188/smsp2-a-serine-protease-secreted-by-the-blood-fluke-pathogen-schistosoma-mansoni-with-anti-hemostatic-properties
#10
Adrian Leontovyč, Lenka Ulrychová, Anthony J O'Donoghue, Jiří Vondrášek, Lucie Marešová, Martin Hubálek, Pavla Fajtová, Marta Chanová, Zhenze Jiang, Charles S Craik, Conor R Caffrey, Michael Mareš, Jan Dvořák, Martin Horn
BACKGROUND: Serine proteases are important virulence factors for many pathogens. Recently, we discovered a group of trypsin-like serine proteases with domain organization unique to flatworm parasites and containing a thrombospondin type 1 repeat (TSR-1). These proteases are recognized as antigens during host infection and may prove useful as anthelminthic vaccines, however their molecular characteristics are under-studied. Here, we characterize the structural and proteolytic attributes of serine protease 2 (SmSP2) from Schistosoma mansoni, one of the major species responsible for the tropical infectious disease, schistosomiasis...
April 20, 2018: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/29676858/effects-of-prebiotic-fiber-xylooligosaccharide-in-adenine-induced-nephropathy-in-mice
#11
Jieping Yang, Qing Li, Susanne M Henning, Jin Zhong, Mark Hsu, Rupo Lee, Jianfeng Long, Brenda Chan, Glenn T Nagami, David Heber, Zhaoping Li
SCOPE: We evaluated the effect of the prebiotic fiber xylooligosaccharide (XOS) on kidney function and gut microbiome in mice with adenine-induced chronic kidney disease (CKD). METHOD AND RESULTS: Mice were fed the control diet containing adenine for three weeks to induce CKD and switched to XOS supplemented (2 or 7%) or control diets for another 3 weeks. Mice with CKD exhibited increased BUN, creatinine and kidney histopathology. XOS significantly reversed kidney injuries in CKD mice...
April 20, 2018: Molecular Nutrition & Food Research
https://www.readbyqxmd.com/read/29676198/blockade-of-at1-type-receptors-for-angiotensin-ii-prevents-cardiac-microvascular-fibrosis-induced-by-chronic-stress-in-sprague-dawley-rats
#12
Lília Taddeo Firoozmand, Andrea Sanches, Nilsa Regina Damaceno-Rodrigues, Juliana Dinéia Perez, Danielle Sanches Aragão, Rodolfo Mattar Rosa, Fernanda Klein Marcondes, Dulce Elena Casarini, Elia Garcia Caldini, Tatiana Sousa Cunha
To test the effects of chronic-stress on the cardiovascular system, the model of chronic mild unpredictable stress (CMS) has been widely used. The CMS protocol consists of the random, intermittent, and unpredictable exposure of laboratory animals to a variety of stressors, during 3 consecutive weeks. In this study, we tested the hypothesis that exposure to the CMS protocol leads to left ventricle microcirculatory remodeling that can be attenuated by angiotensin II receptor blockade. Male Sprague-Dawley rats were randomly assigned into four groups: Control, Stress, Control + losartan, and Stress + losartan (N = 6, each group, losartan: 20 mg/kg/day)...
April 20, 2018: Stress: the International Journal on the Biology of Stress
https://www.readbyqxmd.com/read/29675573/triclosan-evoked-neurotoxicity-involves-nmdar-subunits-with-the-specific-role-of-glun2a-in-caspase-3-dependent-apoptosis
#13
Konrad A Szychowski, Agnieszka Wnuk, Joanna Rzemieniec, Małgorzata Kajta, Teresa Leszczyńska, Anna K Wójtowicz
Triclosan (TCS) is an antimicrobial agent that is used extensively in personal care and in sanitising products. A number of studies have shown the presence of TCS in different human tissues such as blood, adipose tissue, the liver, brain as well as in breast milk and urine. N-Methyl-D-aspartate receptors (NMDARs) are glutamate-gated ion channels that are widely expressed in the central nervous system and which play key roles in excitatory synaptic transmission. There is, however, no data on the involvement of NMDAR subunits in the apoptotic and neurotoxic effects of TCS...
April 19, 2018: Molecular Neurobiology
https://www.readbyqxmd.com/read/29675023/increased-plasma-levels-of-danger-associated-molecular-patterns-are-associated-with-immune-suppression-and-postoperative-infections-in-patients-undergoing-cytoreductive-surgery-and-hyperthermic-intraperitoneal-chemotherapy
#14
Guus P Leijte, Hettie Custers, Jelle Gerretsen, Amon Heijne, Johannes Roth, Thomas Vogl, Gert J Scheffer, Peter Pickkers, Matthijs Kox
Introduction: Danger-associated molecular patterns (DAMPs) can elicit immune responses and may subsequently induce an immune-suppressed state. Previous work showed that increased plasma levels of DAMPs are associated with immune suppression and increased susceptibility toward infections in trauma patients. Like trauma, major surgical procedures, such as cytoreductive surgery (CRS) combined with hyperthermic intraperitoneal chemotherapy (HIPEC), are also thought to cause profound DAMP release...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29675019/enhanced-akt-phosphorylation-of-circulating-b-cells-in-patients-with-activated-pi3k%C3%AE-syndrome
#15
Takaki Asano, Satoshi Okada, Miyuki Tsumura, Tzu-Wen Yeh, Kanako Mitsui-Sekinaka, Yuki Tsujita, Youjiro Ichinose, Akira Shimada, Kunio Hashimoto, Taizo Wada, Kohsuke Imai, Osamu Ohara, Tomohiro Morio, Shigeaki Nonoyama, Masao Kobayashi
Activated PI3Kδ syndrome (APDS) is a primary immunodeficiency characterized by recurrent respiratory tract infections, lymphoproliferation, and defective IgG production. Heterozygous mutations in PIK3CD, PIK3R1 , or PTEN , which are related to the hyperactive phosphoinositide 3-kinase (PI3K) signaling, were recently presented to cause APDS1 or APDS2 (APDSs), or APDS-like (APDS-L) disorder. In this study, we examined the AKT phosphorylation of peripheral blood lymphocytes and monocytes in patients with APDSs and APDS-L by using flow cytometry...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29674449/induction-of-antitumor-cytotoxic-lymphocytes-using-engineered-human-primary-blood-dendritic-cells
#16
Long Wu, Huan Zhang, Yixing Jiang, Robert C Gallo, Hua Cheng
Dendritic cell (DC)-based cancer immunotherapy has achieved modest clinical benefits, but several technical hurdles in DC preparation, activation, and cancer/testis antigen (CTA) delivery limit its broad applications. Here, we report the development of immortalized and constitutively activated human primary blood dendritic cell lines (ihv-DCs). The ihv-DCs are a subset of CD11c+ /CD205+ DCs that constitutively display costimulatory molecules. The ihv-DCs can be genetically modified to express human telomerase reverse transcriptase (hTERT) or the testis antigen MAGEA3 in generating CTA-specific cytotoxic T lymphocytes (CTLs)...
April 19, 2018: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29674278/cassia-grandis-fruit-extract-reduces-the-blood-glucose-level-in-alloxan-induced-diabetic-rats
#17
Ariadna Lafourcade Prada, Jesús Rafael Rodríguez Amado, Hady Keita, Edgar Puente Zapata, Helison Carvalho, Emersom Silva Lima, Tatiane Pereira de Sousa, José Carlos Tavares Carvalho
INTRODUCTION: Cassia grandis Lf fruits are ethnobotanically used for digestive disorders, anemia, and for reducing blood glucose. However, there are no studies about the antidiabetic activity nor the oral toxicity of the plant fruit-extracts. This paper aims to evaluate the hypoglycemic effect of C. grandis fruits extract in vivo, and assess the acute oral toxicity, and sub-acute oral toxicity. The antioxidant activity and the α-glycosidase inhibitor effect were also evaluated. METHODS: The extract was obtained by maceration of the fruit pulp with 70% hydroalcoholic solution (1:2, m:v)...
April 16, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29674267/anti-inflammatory-effect-of-multistrain-probiotic-formulation-l-rhamnosus-b-lactis-and-b-longum
#18
Marzia Sichetti, Stefania De Marco, Rita Pagiotti, Giovanna Traina, Donatella Pietrella
OBJECTIVE: In recent years, a great number of studies have been directed toward the evaluation of gastrointestinal microbiota modulation through the introduction of beneficial microorganisms, also known as probiotics. Many studies have highlighted how this category of bacteria is very important for the good development, functioning, and maintenance of our immune system. There is a delicate balance between the immune system, located under the gut epithelial barrier, and the microbiota, but many factors can induce a disequilibrium that leads to an inflammatory state and dysbiosis...
February 14, 2018: Nutrition
https://www.readbyqxmd.com/read/29674255/topically-applied-azaphenothiazines-inhibit-experimental-psoriasis-in-mice
#19
Jolanta Artym, Maja Kocięba, Ewa Zaczyńska, Iwona Kochanowska, Michał Zimecki, Wojciech Kałas, Anna Fiedorowicz, Alicja Pawlak, Leon Strządała, Małgorzata Jeleń, Beata Morak-Młodawska, Krystian Pluta, Katarzyna Kaleta-Kuratewicz, Jan P Madej, Piotr Kuropka, Jan Kuryszko
The therapeutic efficacy of topically applied azaphenothiazine derivatives: 9-chloro-6-acetylaminobutylquinobenzo[3,2-b][1,4]thiazine (compound 4) and 6-chloroethylureidoethyldiquino[3,2-b;2';3'-e][1,4]thiazine (compound 5) in the amelioration of inflammatory symptoms of imiquimod-induced psoriasis in mice was investigated. Clobederm®, containing clobetasol propioniate, served as a reference drug. The application of the compounds led to thinning of the epidermis and reduction of the cell layers. The suppressive actions of the compounds were even stronger with regard to pathological changes of the dermis...
April 16, 2018: International Immunopharmacology
https://www.readbyqxmd.com/read/29674197/villous-explants-from-preeclamptic-placentas-induce-sflt1-in-pbmcs-an-ex-vivo-co-culture-study
#20
Stephen Kyle Gonzales, Martina Badell, Hanh Cottrell, Bassam Rimawi, Venkataraman Deepak, Neil Sidell, Augustine Rajakumar
OBJECTIVES: Soluble Flt1 (sFlt1) is an anti-angiogenic protein linked to the pathology of preeclampsia (PE). While the placenta serves as the major organ producing sFlt1 during normal pregnancy, peripheral blood mononuclear cells (PBMCs), endothelial cells, and stromal cells also produce sFlt1. The key question is 'what drives the overexpression of sFlt1 observed during PE?' In the present work we show evidence for sFlt1 over-expression in PBMCs due to interaction with placental villi from PE patients...
February 25, 2018: Pregnancy Hypertension
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