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https://www.readbyqxmd.com/read/29661876/antimicrobial-and-antibiofilm-efficacy-of-graphene-oxide-against-chronic-wound-microorganisms
#1
Mara Di Giulio, Romina Zappacosta, Silvia Di Lodovico, Emanuela Di Campli, Gabriella Siani, Antonella Fontana, Luigina Cellini
Chronic wounds represent an increasing problem worldwide. Graphene oxide (GO) has been reported to exhibit strong antibacterial activity towards both Gram-positive and Gram-negative bacteria. The aim of this work was to investigate the in vitro antimicrobial and antibiofilm efficacy of GO against wound pathogens. Staphylococcus aureus PECHA 10, Pseudomonas aeruginosa PECHA 4 and Candida albicans X3 clinical isolates were incubated with 50 mg/L of GO for 2 and 24 h to evaluate the antimicrobial effect. Optical and Atomic force microscopy images were performed to visualize the GO effect on microbial cells...
April 16, 2018: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/29557635/hierarchically-porous-n-doped-cnts-reduced-graphene-oxide-composite-for-promoting-flavin-based-interfacial-electron-transfer-in-microbial-fuel-cells
#2
Xiaoshuai Wu, Yan Qiao, Zhuanzhuan Shi, Wei Tang, Chang Ming Li
Interfacial electron transfer between electroactive biofilm and the electrode is crucial step for microbial fuel cells (MFCs) and other bioelectrochemical systems. Here, a hierarchically porous nitrogen-doped CNTs/reduced graphene oxide (rGO) composite with polyaniline as nitrogen source has been developed for MFCs anode. This composite possesses nitrogen atoms doped surface for improved flavin redox reaction and a three-dimensional (3D) hierarchically porous structure for rich bacterial biofilm growth. The N-CNTs/rGO anode delivers a maximum power density of 1137 mW m-2 in Shewanella putrefaciens CN32 microbial fuel cells, which is 8...
March 20, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29482032/carnosine-graphene-oxide-conjugates-decorated-with-hydroxyapatite-as-promising-nanocarrier-for-icg-loading-with-enhanced-antibacterial-effects-in-photodynamic-therapy-against-streptococcus-mutans
#3
Elham Gholibegloo, Ashkan Karbasi, Maryam Pourhajibagher, Nasim Chiniforush, Ali Ramazani, Tayebeh Akbari, Abbas Bahador, Mehdi Khoobi
Antimicrobial photodynamic therapy (aPDT) has been emerged as a noninvasive strategy to remove bacterial contaminants such as S. mutans from the tooth surface. Photosensitizer (PS), like indocyanine green (ICG), plays a key role in this technique which mainly suffers from the poor stability and concentration-dependent aggregation. An appropriate nanocarrier (NC) with enhanced antibacterial effects could overcome these limitations and improve the efficiency of ICG as a PS. In this study, various ICG-loaded NCs including graphene oxide (GO), GO-carnosine (Car) and GO-Car/Hydroxyapatite (HAp) were synthesized and characterized by Fourier Transform Infrared Spectroscopy (FT-IR), X-ray Diffraction (XRD), Filed Emission Scanning Electron Microscopy (FE-SEM), Energy Dispersive Spectroscopy (EDS), Zeta Potential and Ultraviolet-Visible spectrometry (UV-Vis)...
February 9, 2018: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/29459912/mechanistic-insight-into-the-in-vitro-toxicity-of-graphene-oxide-against-biofilm-forming-bacteria-using-laser-induced-breakdown-spectroscopy
#4
Muhammad Usman Farid, Sanghyun Jeong, Dong Han Seo, Rafay Ahmed, Condon Lau, Nirmal Kumar Gali, Zhi Ning, Alicia Kyoungjin An
While the cytotoxicity of graphene oxide (GO) has been well established, its bactericidal mechanism, however, has yet to be elucidated to advance GO-based biomedical and environmental applications. In an attempt to better understand the bactericidal action of GO, herein we studied the interactions of GO with Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus cells using physical techniques and chemical probes, respectively. In particular, a novel laser-induced breakdown spectroscopy (LIBS) based elemental fingerprint analysis revealed notable differences between viable and non-viable cells based on the difference in the concentration of trace inorganic elements in complex bacterial systems, which reflect cellular membrane integrity...
February 20, 2018: Nanoscale
https://www.readbyqxmd.com/read/29409364/graphene-onto-medical-grade-titanium-an-atom-thick-multimodal-coating-that-promotes-osteoblast-maturation-and-inhibits-biofilm-formation-from-distinct-species
#5
Nileshkumar Dubey, Kassapa Ellepola, Fanny E D Decroix, Julien L P Morin, A H Castro Neto, Chaminda J Seneviratne, Vinicius Rosa
The time needed for the osseointegration of titanium implants is deemed too long. Moreover, the bacterial colonization of their surfaces is a major cause of failure. Graphene can overcome these issues but its wet transfer onto substrates employs hazardous chemicals limiting the clinical applications. Alternatively, dry transfer technique has been developed, but the biological properties of this technique remain unexplored. Here, a dry transfer technique based on a hot-pressing method allowed to coat titanium substrates with high-quality graphene and coverage area >90% with a single transfer...
February 6, 2018: Nanotoxicology
https://www.readbyqxmd.com/read/29357405/combat-biofilm-by-bacteriostatic-aptamer-functionalized-graphene-oxide
#6
Biyao Mao, Lijuan Cheng, Sheng Wang, Jiaqi Zhou, Le Deng
Biofilms are the main reason for a large number deaths and high health costs. Their better protection compared to planktonic form against conventional antibiotics leads to poor treatment efficiency. Nanoagent-targeted delivery is a promising avenue for disease therapeutic, but its application targeting biofilms has not been reported currently. The roles, if any, of aptamers acting as delivery carrier and targeting factor, the graphene oxide (GO), and GO modified with aptamers against biofilms were then systematically evaluated...
January 22, 2018: Biotechnology and Applied Biochemistry
https://www.readbyqxmd.com/read/29321058/graphene-oxide-conjugated-with-polymers-a-study-of-culture-condition-to-determine-whether-a-bacterial-growth-stimulant-or-an-antimicrobial-agent
#7
Ping-Ching Wu, Hua-Han Chen, Shih-Yao Chen, Wen-Lung Wang, Kun-Lin Yang, Chia-Hung Huang, Hui-Fang Kao, Jui-Cheng Chang, Chih-Li Lilian Hsu, Jiu-Yao Wang, Ting-Mao Chou, Wen-Shuo Kuo
BACKGROUND: The results showed that the deciding factor is the culture medium in which the bacteria and the graphene oxide (GO) are incubated at the initial manipulation step. These findings allow better use of GO and GO-based materials more and be able to clearly apply them in the field of biomedical nanotechnology. RESULTS: To study the use of GO sheets applied in the field of biomedical nanotechnology, this study determines whether GO-based materials [GO, GO-polyoxyalkyleneamine (POAA), and GO-chitosan] stimulate or inhibit bacterial growth in detail...
January 10, 2018: Journal of Nanobiotechnology
https://www.readbyqxmd.com/read/29273994/effects-of-low-level-engineered-nanoparticles-on-the-quorum-sensing-of-pseudomonas-aeruginosa-pao1
#8
Na Li, Lijia Wang, Huicong Yan, Meizhen Wang, Dongsheng Shen, Jun Yin, Jiali Shentu
The toxicity of engineered nanoparticles (ENPs) on bacteria has aroused much interest. However, few studies have focused on the effects of low-level ENPs on bacterial group behaviors that are regulated by quorum sensing (QS). Herein, we investigated the effects of nine ENPs (Ag, Fe, ZnO, TiO2 , SiO2 , Fe2 O3 , single-wall carbon nanotubes (SWCNTs), graphene oxide (GO), and C60 ) on QS in Pseudomonas aeruginosa PAOl. An ENP concentration of 100 μg L-1 did not impair bacterial growth. However, concentrations of 100 μg L-1 of Ag and GO ENPs induced significant increases in 3OC12 -HSL in the culture and significantly promoted protease production and biofilm formation of PAO1...
March 2018: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/29241007/sulfur-doped-laser-induced-porous-graphene-derived-from-polysulfone-class-polymers-and-membranes
#9
Swatantra P Singh, Yilun Li, Jibo Zhang, James M Tour, Christopher J Arnusch
Graphene based materials have profoundly impacted research in nanotechnology, and this has significantly advanced biomedical, electronics, energy, and environmental applications. Laser-induced graphene (LIG) is made photothermally and has enabled a rapid route for graphene layers on polyimide surfaces. However, polysulfone (PSU), poly(ether sulfone) (PES), and polyphenylsulfone (PPSU) are highly used in numerous applications including medical, energy, and water treatment and they are critical components of polymer membranes...
January 23, 2018: ACS Nano
https://www.readbyqxmd.com/read/29233187/graphene-based-dental-adhesive-with-anti-biofilm-activity
#10
Agnese Bregnocchi, Elena Zanni, Daniela Uccelletti, Fabrizio Marra, Domenico Cavallini, Francesca De Angelis, Giovanni De Bellis, Maurizio Bossù, Gaetano Ierardo, Antonella Polimeni, Maria Sabrina Sarto
BACKGROUND: Secondary caries are considered the main cause of dental restoration failure. In this context, anti-biofilm and bactericidal properties are desired in dental materials against pathogens such as Streptococcus mutans. To this purpose, graphene based materials can be used as fillers of polymer dental adhesives. In this work, we investigated the possibility to use as filler of dental adhesives, graphene nanoplatelets (GNP), a non toxic hydrophobic nanomaterial with antimicrobial and anti-biofilm properties...
December 12, 2017: Journal of Nanobiotechnology
https://www.readbyqxmd.com/read/29216517/go-agcl-ag-nanocomposites-with-enhanced-visible-light-driven-catalytic-properties-for-antibacterial-and-biofilm-disrupting-applications
#11
Xinhuan Wang, Qiusen Han, Ning Yu, Tian Wang, Chen Wang, Rong Yang
Nanomaterials with visible light-driven photocatalytic activity have attracted much attention due to their excellent abilities in degradation of various organic pollutants as well as inactivating bacteria. Herein, graphene oxide (GO) enwrapped silver chloride/silver (AgCl/Ag) nanocomposites with high visible light absorption were designed and fabricated as efficient antibacterial agents. AgCl NPs were synthesized in the presence of GO first and Ag NPs were coated on AgCl surface by heat reduction to form GO-AgCl/Ag nanocomposites...
February 1, 2018: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/29143262/influences-of-graphene-oxide-on-biofilm-formation-of-gram-negative-and-gram-positive-bacteria
#12
Chao Song, Chun-Miao Yang, Xue-Fei Sun, Peng-Fei Xia, Jing Qin, Bei-Bei Guo, Shu-Guang Wang
In this study, we evaluated the influences of graphene oxide (GO) on biofilm formation. Escherichia coli MG1655 and Bacillus subtilis 168 were used as models for Gram-negative and Gram-positive bacteria. The growth profiles and viability assays indicated that GO exhibited a high antibacterial activity, of which the negative effects on bacteria growth raised with the increasing GO concentration. The antibacterial activity of GO was mainly attributed to the membrane stress and ROS-independent oxidative stress...
January 2018: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/29125543/a-mediated-bod-biosensor-based-on-immobilized-b-subtilis-on-three-dimensional-porous-graphene-polypyrrole-composite
#13
Jingfang Hu, Yueqi Li, Guowei Gao, Shanhong Xia
We have developed a novel mediated biochemical oxygen demand (BOD) biosensor based on immobilized Bacillus subtilis ( B. subtilis ) on three-dimensional (3D) porous graphene-polypyrrole (rGO-PPy) composite. The 3D porous rGO-PPy composite was prepared using hydrothermal method following with electropolymerization. Then the 3D porous rGO-PPy composite was used as a support for immobilizing negatively charged B. subtilis denoted as rGO-PPy- B through coordination and electrostatic interaction. Further, the prepared rGO-PPy- B was used as a microbial biofilm for establishing a mediated BOD biosensor with ferricyanide as an electronic acceptor...
November 10, 2017: Sensors
https://www.readbyqxmd.com/read/29062127/graphene-oxide-electrodeposited-electrode-enhances-start-up-and-selective-enrichment-of-exoelectrogens-in-bioelectrochemical-systems
#14
R M Alonso, M I San-Martín, A Sotres, A Escapa
This study seeks to assess the impact that the anodic electrodeposition of graphene oxide (GO) has on the start-up process and on the development of microbial communities on the anode of BESs. The GO electrodeposited electrodes were characterised in abiotic conditions to verify the extent of the modification and were then transferred to a bioelectrochemical reactor. Results showed that the modified electrode allowed for a reduced start-up time compared to the control electrode. After three months, high throughput sequencing was performed, revealing that electrochemically reduced graphene oxide acts as a selective agent toward exoelectrogenic bacteria as Geobacter...
October 23, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29023399/novel-anti-tuberculosis-nanodelivery-formulation-of-ethambutol-with-graphene-oxide
#15
Bullo Saifullah, Alina Chrzastek, Arundhati Maitra, Bullo Naeemullah, Sharida Fakurazi, Sanjib Bhakta, Mohd Zobir Hussein
Tuberculosis (TB) is a bacterial disease responsible for millions of infections and preventable deaths each year. Its treatment is complicated by patients' noncompliance due to dosing frequency, lengthy treatment, and adverse side effects associated with current chemotherapy. However, no modifications to the half-a-century old standard chemotherapy have been made based on a nanoformulation strategy to improve pharmacokinetic efficacy. In this study, we have designed a new nanodelivery formulation, using graphene oxide as the nanocarrier, loaded with the anti-TB antibiotic, ethambutol...
October 12, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28925946/development-and-biocompatibility-evaluation-of-photocatalytic-tio%C3%A2-reduced-graphene-oxide-based-nanoparticles-designed-for-self-cleaning-purposes
#16
Ionela Cristina Nica, Miruna S Stan, Marcela Popa, Mariana Carmen Chifiriuc, Gratiela G Pircalabioru, Veronica Lazar, Iuliana Dumitrescu, Lucian Diamandescu, Marcel Feder, Mihaela Baibarac, Marin Cernea, Valentin Adrian Maraloiu, Traian Popescu, Anca Dinischiotu
Graphene is widely used in nanotechnologies to amplify the photocatalytic activity of TiO₂, but the development of TiO₂/graphene composites imposes the assessment of their risk to human and environmental health. Therefore, reduced graphene oxide was decorated with two types of TiO₂ particles co-doped with 1% iron and nitrogen, one of them being obtained by a simultaneous precipitation of Ti(3+) and Fe(3+) ions to achieve their uniform distribution, and the other one after a sequential precipitation of these two cations for a higher concentration of iron on the surface...
September 19, 2017: Nanomaterials
https://www.readbyqxmd.com/read/28867453/the-effect-of-indocyanine-green-loaded-on-a-novel-nano-graphene-oxide-for-high-performance-of-photodynamic-therapy-against-enterococcus-faecalis
#17
Tayebeh Akbari, Maryam Pourhajibagher, Farzaneh Hosseini, Nasim Chiniforush, Elham Gholibegloo, Mehdi Khoobi, Sima Shahabi, Abbas Bahador
BACKGROUND: Recently developed photodynamic therapy (PDT) has gained attention for achieving effective root canal disinfection. Using an optimized nontoxic photosensitizer (PS), such as indocyanine green (ICG), is an imperative part of this technique. Therefore, the objective of the current study was to improve ICG photodynamic properties through incorporation of ICG into nano-graphene oxide (NGO) in order to produce NGO-ICG as a new PS and also to assess the antimicrobial effects of NGO-ICG against Enterococcus faecalis after photodynamic therapy...
December 2017: Photodiagnosis and Photodynamic Therapy
https://www.readbyqxmd.com/read/28764786/evaluation-of-the-antibacterial-power-and-biocompatibility-of-zinc-oxide-nanorods-decorated-graphene-nanoplatelets-new-perspectives-for-antibiodeteriorative-approaches
#18
Elena Zanni, Erika Bruni, Chandrakanth Reddy Chandraiahgari, Giovanni De Bellis, Maria Grazia Santangelo, Maurizio Leone, Agnese Bregnocchi, Patrizia Mancini, Maria Sabrina Sarto, Daniela Uccelletti
BACKGROUND: Nanotechnologies are currently revolutionizing the world around us, improving the quality of our lives thanks to a multitude of applications in several areas including the environmental preservation, with the biodeterioration phenomenon representing one of the major concerns. RESULTS: In this study, an innovative nanomaterial consisting of graphene nanoplatelets decorated by zinc oxide nanorods (ZNGs) was tested for the ability to inhibit two different pathogens belonging to bacterial genera frequently associated with nosocomial infections as well as biodeterioration phenomenon: the Gram-positive Staphylococcus aureus and the Gram-negative Pseudomonas aeruginosa...
August 1, 2017: Journal of Nanobiotechnology
https://www.readbyqxmd.com/read/28704738/high-efficiency-microbial-electrosynthesis-of-acetate-from-carbon-dioxide-by-a-self-assembled-electroactive-biofilm
#19
Tian-Shun Song, Hongkun Zhang, Haixia Liu, Dalu Zhang, Haoqi Wang, Yang Yang, Hao Yuan, Jingjing Xie
Microbial electrosynthesis (MES) is a biocathode-driven process, producing high-value chemicals from CO2. Here, an in situ self-assembled graphene oxide (rGO)/biofilm was constructed, in MES, for high efficient acetate production. GO has been successfully reduced by electroautotrophic bacteria for the first time. An increase, of 1.5 times, in the volumetric acetate production rate, was obtained by self-assembling rGO/biofilm, as compared to the control group. In MES with rGO/biofilm, a volumetric acetate production rate of 0...
November 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28677651/effect-of-precursor-on-antifouling-efficacy-of-vertically-oriented-graphene-nanosheets
#20
Karthika Prasad, Chaturanga D Bandara, Shailesh Kumar, Gurinder Pal Singh, Bastian Brockhoff, Kateryna Bazaka, Kostya Ken Ostrikov
Antifouling efficacy of graphene nanowalls, i.e., substrate-bound vertically-oriented graphene nanosheets, has been demonstrated against biofilm-forming Gram-positive and Gram-negative bacteria. Where graphene nanowalls are typically prepared using costly high-temperature synthesis from high-purity carbon precursors, large-scale applications demand efficient, low-cost processes. The advancement of plasma enabled synthesis techniques in the production of nanomaterials has opened a novel and effective method for converting low-cost natural waste resources to produce nanomaterials with a wide range of applications...
July 4, 2017: Nanomaterials
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