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https://www.readbyqxmd.com/read/28631614/3d-printed-bioceramic-scaffolds-with-antibacterial-and-osteogenic-activity
#1
Yongliang Zhang, Dong Zhai, Mengchi Xu, Qingqiang Yao, Huiying Zhu, Jiang Chang, Chengtie Wu
Bacterial infection poses a significant risk with the wide application of bone graft materials. Designing bone grafts with good antibacterial performance and excellent bone-forming activity is of particular significance for bone tissue engineering. In our study, a 3D printing method was used to prepare β-tricalcium phosphate (β-TCP) bioceramic scaffolds. Silver (Ag) nanoparticles were uniformly dispersed on graphene oxide (GO) to form a homogeneous nanocomposite (named Ag@GO) with different Ag-to-graphene oxide mass ratios, with this being synthesized via the liquid chemical reduction approach...
June 20, 2017: Biofabrication
https://www.readbyqxmd.com/read/28624620/agbr-nanoparticles-3d-nitrogen-doped-graphene-hydrogel-for-fabricating-all-solid-state-luminol-electrochemiluminescence-escherichia-coli-aptasensors
#2
Nan Hao, Xuan Zhang, Zhou Zhou, Rong Hua, Ying Zhang, Qian Liu, Jing Qian, Henan Li, Kun Wang
It is necessary to develop rapid, simple and accurate detection method for Escherichia coli (E. coli) due to its widely distributed pathogenic bacteria. Herein, we prepared AgBr nanoparticles (NPs) anchored 3D nitrogen-doped graphene hydrogel (3DNGH) nanocomposites with an exceptionally large accessible surface by a simple hydrothermal approach. The as-prepared 3DNGH porous nanocomposite not only showed better conductivity than that of 3D graphene due to introducing nitrogen element into graphene framework, but also provided a high loading volume for immobilizing luminol...
June 13, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28582974/breathable-vapor-toxicant-barriers-based-on-multilayer-graphene-oxide
#3
Ruben Spitz Steinberg, Michelle Cruz, Naser G A Mahfouz, Yang Qiu, Robert H Hurt
There is tremendous interest in graphene-based membranes as protective molecular barriers or molecular sieves for separation technologies. Graphene oxide (GO) films in the dry state are known to be effective barriers for molecular transport and to expand in the presence of moisture to create enlarged intersheet gallery spaces that allow rapid water permeation. Here we explore an application for GO membranes as water-breathable barrier layers for personal protective equipment, which are designed to allow outward perspiration while protecting the wearer from chemical toxicants or biochemical agents in the local environment...
June 8, 2017: ACS Nano
https://www.readbyqxmd.com/read/28575993/graphene-oxide-nanoribbons-as-nanomaterial-for-bone-regeneration-effects-on-cytotoxicity-gene-expression-and-bactericidal-effect
#4
R Ricci, N C S Leite, N S da-Silva, C Pacheco-Soares, R A Canevari, F R Marciano, T J Webster, A O Lobo
Graphene oxide nanoribbons (O-GNR) surges as an interesting nanomaterial for biomedical applications due to feasibility to incorporate functional groups and possible bactericidal properties. Herein, high concentrations of O-GNR were biologically evaluated using human osteoblast cells and gram positive and negative bacteria. Briefly, our goal were to evaluate: (1) synthetic pathway, (2) characterization and (3) effects of O-GNR composition and structural factors as a new approach for biomedical applications...
September 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28570887/bacterial-community-shift-and-incurred-performance-in-response-to-in-situ-microbial-self-assembly-graphene-and-polarity-reversion-in-microbial-fuel-cell
#5
Junfeng Chen, Lihua Zhang, Yongyou Hu, Wantang Huang, Zhuyu Niu, Jian Sun
In this work, bacterial community shift and incurred performance of graphene modified bioelectrode (GM-BE) in microbial fuel cell (MFC) were illustrated by high throughput sequencing technology and electrochemical analysis. The results showed that Firmicutes occupied 48.75% in graphene modified bioanode (GM-BA), while Proteobacteria occupied 62.99% in graphene modified biocathode (GM-BC), both were dominant bacteria in phylum level respectively. Typical exoelectrogens, including Geobacter, Clostridium, Pseudomonas, Geothrix and Hydrogenophaga, were counted 26...
May 20, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28554055/graphene-oxide-as-an-efficient-antimicrobial-nanomaterial-for-eradicating-multi-drug-resistant-bacteria-in-vitro-and-in-vivo
#6
Xu Wu, Shirui Tan, Yuqian Xing, Qinqin Pu, Min Wu, Julia Xiaojun Zhao
Graphene is a novel two-dimensional nanomaterial with a growing number of practical applications across numerous fields. In this work, we explored potential biomedical applications of graphene oxide (GO) by systematically studying antibacterial capacity of GO in both macrophages and animal models. Three types of bacteria, including Klebsiella pneumoniae (Kp), Escherichia coli (E. coli) and P. aeruginosa (Pa) were used for in vitro study. Kp was also selected as a representative multidrug resistant (MDR) bacterium for in vivo study...
May 17, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28549325/graphene-oxide-in-the-water-environment-could-affect-tetracycline-antibiotic-resistance
#7
Mei-Ting Guo, Guo-Sheng Zhang
In recent years, the influence of new materials like nanoparticles in the water environment on biological substances has been widely studied. Antibiotic resistance genes (ARGs) represent a new type of pollutant in the environment. Graphene oxide (GO), as a nano material, because of its unique structure, may have an impact on antibiotic resistance bacteria (ARB) and ARGs; however the research in this area is rarely reported. Therefore, this study mainly investigated the effects of GO on bacterial antibiotic resistance...
May 3, 2017: Chemosphere
https://www.readbyqxmd.com/read/28534395/hyaluronic-acid-templated-ag-nanoparticles-graphene-oxide-composites-for-synergistic-therapy-of-bacteria-infection
#8
Xiang Ran, Ye Du, Zhenzhen Wang, Huan Wang, Fang Pu, Jinsong Ren, Xiaogang Qu
Developing methods of decreasing the harm to cell and increasing the antibacterial efficiency is becoming a potential topic of medical treatments. We demonstrated a hyaluronidase-triggered photothermal platform for killing bacteria based on silver nanoparticles (AgNPs) and graphene oxide (GO). The property of the hyaluronidase (HAase)-triggered release provided excellent antibacterial activity against Staphylococcus aureus. Upon illumination of NIR light, the GO-based nanomaterials locally raised the temperature, resulting in high mortality of bacteria...
June 5, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28531860/boosting-biomethane-yield-and-production-rate-with-graphene-the-potential-of-direct-interspecies-electron-transfer-in-anaerobic-digestion
#9
Richen Lin, Jun Cheng, Jiabei Zhang, Junhu Zhou, Kefa Cen, Jerry D Murphy
Interspecies electron transfer between bacteria and archaea plays a vital role in enhancing energy efficiency of anaerobic digestion (AD). Conductive carbon materials (i.e. graphene nanomaterial and activated charcoal) were assessed to enhance AD of ethanol (a key intermediate product after acidogenesis of algae). The addition of graphene (1.0g/L) resulted in the highest biomethane yield (695.0±9.1mL/g) and production rate (95.7±7.6mL/g/d), corresponding to an enhancement of 25.0% in biomethane yield and 19...
September 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28504463/magnetocatalytic-graphene-quantum-dots-janus-micromotors-for-bacterial-endotoxin-detection
#10
Beatriz Jurado-Sánchez, Marta Pacheco, Jaime Rojo, Alberto Escarpa
Magnetocatalytic hybrid Janus micromotors encapsulating phenylboronic acid (PABA) modified graphene quantum dots (GQDs) are described herein as ultrafast sensors for the detection of deadly bacteria endotoxins. A bottom-up approach was adopted to synthesize an oil-in-water emulsion containing the GQDs along with a high loading of platinum and iron oxide nanoparticles on one side of the Janus micromotor body. The two different "active regions" enable highly efficient propulsion in the presence of hydrogen peroxide or magnetic actuation without the addition of a chemical fuel...
May 15, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28484209/synergic-bactericidal-effects-of-reduced-graphene-oxide-and-silver-nanoparticles-against-gram-positive-and-gram-negative-bacteria
#11
Karthika Prasad, G S Lekshmi, Kola Ostrikov, Vanessa Lussini, James Blinco, Mandhakini Mohandas, Krasimir Vasilev, Steven Bottle, Kateryna Bazaka, Kostya Ostrikov
Reduced graphene oxide (rGO) is a promising antibacterial material, the efficacy of which can be further enhanced by the addition of silver nanoparticles (nAg). In this study, the mechanisms of antibacterial activity of rGO-nAg nanocomposite against several important human pathogenic multi-drug resistant bacteria, namely Gram-positive coccal Staphylococcus aureus and Gram-negative rod-shaped Escherichia coli and Proteus mirabilis are investigated. At the same concentration (100 µg/ml), rGO-nAg nanocomposite was significantly more effective against all three pathogens than either rGO or nAg...
May 8, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28455502/bio-engineered-graphene-based-cage-for-efficient-local-enrichment-and-biodegradation-of-aqueous-organic-wastes
#12
Jixiang Fan, Dongyun Chen, Najun Li, Qingfeng Xu, Hua Li, Jinghui He, Jianmei Lu
Microorganism immobilization has attracted great attention as a traditional method to overcome aqueous organic wastes containing N, N-dimethylformamide (DMF). In this approach, graphene oxide was modified with functional polymer firstly to obtain micro-composites material (PGO), and then the prepared composites were deposited on the surface of copper mesh (CM) to block the meshes and CM@PGO was achieved. Moreover, cage-shaped model was designed based on CM@PGO and P. denitrificans was packed inside the cage for batch experiments...
April 28, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28442744/biomimetic-antimicrobial-cloak-by-graphene-oxide-agar-hydrogel
#13
Massimiliano Papi, Valentina Palmieri, Francesca Bugli, Marco De Spirito, Maurizio Sanguinetti, Carlotta Ciancico, Maria Chiara Braidotti, Silvia Gentilini, Luca Angelani, Claudio Conti
Antibacterial surfaces have an enormous economic and social impact on the worldwide technological fight against diseases. However, bacteria develop resistance and coatings are often not uniform and not stable in time. The challenge is finding an antibacterial coating that is biocompatible, cost-effective, not toxic, and spreadable over large and irregular surfaces. Here we demonstrate an antibacterial cloak by laser printing of graphene oxide hydrogels mimicking the Cancer Pagurus carapace. We observe up to 90% reduction of bacteria cells...
December 5, 2016: Scientific Reports
https://www.readbyqxmd.com/read/28422486/stable-nanocomposite-based-on-pegylated-and-silver-nanoparticles-loaded-graphene-oxide-for-long-term-antibacterial-activity
#14
Rongtao Zhao, Min Lv, Yang Li, Mingxuan Sun, Wen Kong, Lihua Wang, Shiping Song, Chunhai Fan, Leili Jia, Shaofu Qiu, Yansong Sun, Hongbin Song, Rongzhang Hao
The increasing occurrence of antibiotic-resistant pathogens, especially superbugs, is compromising the efficacy of traditional antibiotics. Silver nanoparticles (AgNPs) loaded graphene oxide (GO) nanocomposite (GO-Ag) has drawn great interest as a promising alternative antibacterial material. However, GO-Ag nanocomposite often irreversibly aggregates in physiological solutions, severely influencing its antibacterial capacity and practical application. Herein, a PEGylated and AgNPs loaded GO nanocomposite (GO-PEG-Ag) is synthesized through a facile approach utilizing microwave irradiation, while avoiding extra reducing agents...
April 28, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28395161/antibacterial-potential-of-electrochemically-exfoliated-graphene-sheets
#15
Zoran M Marković, Danka M Matijašević, Vladimir B Pavlović, Svetlana P Jovanović, Ivanka D Holclajtner-Antunović, Zdenko Špitalský, Matej Mičušik, Miroslav D Dramićanin, Dušan D Milivojević, Miomir P Nikšić, Biljana M Todorović Marković
Electrochemically exfoliated graphene is functionalized graphene with potential application in biomedicine. Two most relevant biological features of this material are its electrical conductivity and excellent water dispersibility. In this study we have tried to establish the correlation between graphene structure and its antibacterial properties. The exfoliation process was performed in a two electrode-highly oriented pyrolytic graphite electrochemical cell. Solution of ammonium persulfate was used as an electrolyte...
April 2, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/28345852/layer-number-dependent-antibacterial-and-osteogenic-behaviors-of-graphene-oxide-electrophoretic-deposited-on-titanium
#16
Jiajun Qiu, Hao Geng, Donghui Wang, Shi Qian, Hongqin Zhu, Yuqin Qiao, Wenhao Qian, Xuanyong Liu
Graphene oxide has attracted widespread attention in the biomedical fields due to its excellent biocompatibility. Herein we investigated the layer-number dependent antibacterial and osteogenic behaviors of graphene oxide in biointerfaces. Graphene oxide with different layer numbers was deposited on the titanium surfaces by cathodal electrophoretic deposition with varied deposition voltages. The initial cell adhesion and spreading, cell proliferation, and osteogenic differentiation were observed from all the samples using rat bone mesenchymal stem cells...
March 31, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28334626/label-free-electrochemiluminescent-biosensor-for-rapid-and-sensitive-detection-of-pseudomonas-aeruginosa-using-phage-as-highly-specific-recognition-agent
#17
Huan Yue, Yong He, Enci Fan, Lin Wang, Shuguang Lu, Zhifeng Fu
A virulent phage named as PaP1 was isolated from hospital sewage based on a lambda phage isolation protocol. This phage showed a strong and highly specific binding ability to Pseudomonas aeruginosa (P. aeruginosa). Using this isolated phage as a recognition agent, a novel electrochemiluminescent (ECL) biosensor was developed for label-free detection of P. aeruginosa. The biosensor was fabricated through depositing phage-conjugated carboxyl graphene onto the surface of a glass carbon electrode. After specific binding of the host bacteria through the adsorption of P...
March 18, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28329722/antibacterial-properties-of-amino-acid-functionalized-silver-nanoparticles-decorated-on-graphene-oxide-sheets
#18
Kumudini Chandraker, Rekha Nagwanshi, S K Jadhav, Kallol K Ghosh, Manmohan L Satnami
Graphene oxide (GO) sheets decorated with amino acid L-cysteine (L-cys) functionalized silver nanoparticles (GO-L-cys-Ag) was synthesized by AgNO3, trisodium citrate, and NaBH4. GO-L-cys-Ag nanocomposite was characterized by transmission electron microscopy (TEM), Fourier transform infrared (FTIR) spectra, ultraviolet-visible (UV-vis) absorption spectra, which demonstrated that a diameter of L-cys-AgNPs compactly deposited on GO. Antibacterial activity tests of GO-L-cys-Ag nanocomposite were carried out using Escherichia coli MTCC 1687 and Staphylococcus aureus MTCC 3160 as model strains of Gram-negative and Gram-positive bacteria, respectively...
March 16, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/28254332/anti-bacterial-activity-of-graphene-oxide-as-a-new-weapon-nanomaterial-to-combat-multidrug-resistance-bacteria
#19
REVIEW
Mohammadreza Yousefi, Mehdi Dadashpour, Maryam Hejazi, Mohammad Hasanzadeh, Behzad Behnam, Miguel de la Guardia, Nasrin Shadjou, Ahad Mokhtarzadeh
Antibiotic resistance in microbial pathogens has become a serious health problem in the world. The increasing spread of hospital acquired infections especially in immunocompromised and cancer patients caused by multidrug-resistant (MDR) microbial pathogens is restricting the choices for impressive antibiotic therapy. So many efforts have been made to develop new compounds with antimicrobial activity. In recent years, nanoparticles, particularly graphene oxide (GO) nanoparticles have found many applications in various fields, including antibacterial action, pathogens bio detection, cancer therapy, and drug and gene delivery...
May 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28198615/antibacterial-activities-of-graphene-oxide-molybdenum-disulfide-nanocomposite-films
#20
Tae In Kim, Buki Kwon, Jonghee Yoon, Ick-Joon Park, Gyeong Sook Bang, YongKeun Park, Yeon-Soo Seo, Sung-Yool Choi
Two-dimensional (2D) nanomaterials, such as graphene-based materials and transition metal dichalcogenide (TMD) nanosheets, are promising materials for biomedical applications owing to their remarkable cytocompatibility and physicochemical properties. On the basis of their potent antibacterial properties, 2D materials have potential as antibacterial films, wherein the 2D nanosheets are immobilized on the surface and the bacteria may contact with the basal planes of 2D nanosheets dominantly rather than contact with the sharp edges of nanosheets...
February 27, 2017: ACS Applied Materials & Interfaces
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