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https://www.readbyqxmd.com/read/29459912/mechanistic-insight-into-the-in-vitro-toxicity-of-graphene-oxide-against-biofilm-forming-bacteria-using-laser-induced-breakdown-spectroscopy
#1
Muhammad Usman Farid, Sanghyun Jeong, Dong Han Seo, Rafay Ahmed, Condon Lau, Nirmal Kumar Gali, Zhi Ning, Alicia Kyoungjin An
While the cytotoxicity of graphene oxide (GO) has been well established, its bactericidal mechanism, however, has yet to be elucidated to advance GO-based biomedical and environmental applications. In an attempt to better understand the bactericidal action of GO, herein we studied the interactions of GO with Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus cells using physical techniques and chemical probes, respectively. In particular, a novel laser-induced breakdown spectroscopy (LIBS) based elemental fingerprint analysis revealed notable differences between viable and non-viable cells based on the difference in the concentration of trace inorganic elements in complex bacterial systems, which reflect cellular membrane integrity...
February 20, 2018: Nanoscale
https://www.readbyqxmd.com/read/29458638/bio-reduction-of-graphene-oxide-using-sulfate-reducing-bacteria-and-its-implication-on-anti-biocorrosion
#2
Tian-Shun Song, Wei-Min Tan, Jingjing Xie
In this paper, we developed an environmental friendly, cost effective, simple and green approach to reduce graphene oxide (GO) by a sulfate-reducing bacterium Desulfovibrio desulfuricans. The D. desulfuricans reduces exfoliated GO to reduced graphene oxide (rGO) at 25 °C in an aqueous solution without any toxic and environmentally harmful reducing agents. The rGO was characterized with X-ray Diffraction, Fourier Transform Infrared Spectroscopy, Scanning Electron Microscopy, Transmission Electron Microscope, X-ray Photoelectron Spectroscopy and Raman Spectroscopy...
August 1, 2018: Journal of Nanoscience and Nanotechnology
https://www.readbyqxmd.com/read/29455524/multiwall-carbon-nanotubes-induce-more-pronounced-transcriptomic-responses-in-pseudomonas-aeruginosa-pg201-than-graphene-exfoliated-boron-nitride-or-carbon-black
#3
Monika Mortimer, Naresh Devarajan, Dong Li, Patricia A Holden
Carbonaceous and boron nitride (BN) nanomaterials have similar applications and hydrophobic properties suggesting common release pathways and exposure to bacteria. While high nanomaterial concentrations can be bactericidal or growth-inhibitory, little is known regarding bacterial transcriptional responses to non-growth-inhibitory nanomaterial concentrations. Here, using one strain of Pseudomonas aeruginosa-a clinically and environmentally important bacterial taxon-we analyzed the comparative transcriptomic response to carbonaceous or BN nanomaterials...
February 19, 2018: ACS Nano
https://www.readbyqxmd.com/read/29442896/fabrication-of-silver-decorated-graphene-oxide-composite-for-photocatalytic-inactivation-of-escherichia-coli
#4
Huixuan Zhang, Xiaoyong Deng, Qiuling Ma, Yuqi Cui, Xiuwen Cheng, Mingzheng Xie, Xiaoli Li, Qingfeng Cheng
In this study, silver decorated graphene oxide (Ag/GO) composite was fabricated through a reduction process in the presence of potassium borohydride solution. Subsequently, physicochemical properties of the resulting Ag/GO composite were studied by scanning electron microscope, X-ray diffraction, Raman spectra, Fourier transformation infrared spectroscopy and UV-visible diffuse reflectance spectrum. Results indicated that Ag species existed in the form of Ag0, which greatly facilitated the visible light absorbance ability...
April 1, 2018: Journal of Nanoscience and Nanotechnology
https://www.readbyqxmd.com/read/29442724/bio-inspired-growth-of-silver-nanoparticles-on-2d-material-s-scaffolds-as-heterostructures-with-their-enhanced-antibacterial-property
#5
Guanhui Gao, Lingwei Wang, Peng Yang, Liang Huang, Xiaobin Xie, Jingsong Wu, Chen Qiu, Lintao Cai
We developed a facile and green bio-inspired strategy to fabricate silver nano-particles growth in-situ on different scaffolds materials, building novel heterostructures for promoting their antibacterial activities and durability. Firstly, fluorinated graphene oxide (FGO) nanosheets, layered molybdenum disulfide (MoS2), and layered tungsten disulfide (WS2) were exfoliated by chemical liquid with intense sonication. And silicon dioxide (SiO2) nano-spheres were prepared via wet chemical method. Then, silver nanoparticles were grown onto those surfaces of layered nanosheets and nano-spheres, hybridizing three dimensional hetero-architectures...
June 1, 2018: Journal of Nanoscience and Nanotechnology
https://www.readbyqxmd.com/read/29409364/graphene-onto-medical-grade-titanium-an-atom-thick-multimodal-coating-that-promotes-osteoblast-maturation-and-inhibits-biofilm-formation-from-distinct-species
#6
Nileshkumar Dubey, Kassapa Ellepola, Fanny E D Decroix, Julien L P Morin, A H Castro Neto, Chaminda J Seneviratne, Vinicius Rosa
The time needed for the osseointegration of titanium implants is deemed too long. Moreover, the bacterial colonization of their surfaces is a major cause of failure. Graphene can overcome these issues but its wet transfer onto substrates employs hazardous chemicals limiting the clinical applications. Alternatively, dry transfer technique has been developed, but the biological properties of this technique remain unexplored. Here, a dry transfer technique based on a hot-pressing method allowed to coat titanium substrates with high-quality graphene and coverage area >90% with a single transfer...
February 6, 2018: Nanotoxicology
https://www.readbyqxmd.com/read/29388390/acute-oral-administration-of-single-walled-carbon-nanotubes-increases-intestinal-permeability-and-inflammatory-responses-association-with-the-changes-in-gut-microbiota-in-mice
#7
Hanqing Chen, Ruifang Zhao, Bing Wang, Lingna Zheng, Hong Ouyang, Hailong Wang, Xiaoyan Zhou, Dan Zhang, Zhifang Chai, Yuliang Zhao, Weiyue Feng
With the increasing production and widespread potential applications of single-walled carbon nanotubes (SWCNTs), the possible impacts of oral administration of SWCNTs on gastrointestinal tract at currently occupational exposure limits and potential biomedical applications should be concerned. To address the concerns, mice are orally administrated of SWCNTs at doses of 0.05, 0.5, and 2.5 mg kg-1 body weight per day for 7 d. The investigation shows that SWCNT treatment had promoted intestinal injuries at the acute dose of 2...
February 1, 2018: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/29378271/a-new-tactics-for-the-detection-of-s-aureus-via-paper-based-geno-interface-incorporated-with-graphene-nano-dots-and-zeolites
#8
Ashish Mathur, Rathin Gupta, Sidharth Kondal, Shikha Wadhwa, Ramesh N Pudake, Shivani Verma, Ruby Kansal, C S Pundir, Jagriti Narang
Staphylococcus aureus (S. aureus) is a pathogenic bacteria which causes infectious diseases and food poisoning. Current diagnostic methods for infectious disease require sophisticated instruments, long analysis time and expensive reagents which restrict their application in resource-limited settings. Electrochemical paper based analytical device (EPAD) was developed by integrating graphene nano dots (GNDs) and zeolite (Zeo) using specific DNA probe. The ssDNA/GNDs-Zeo modified paper based analytical device (PAD) was characterized using cyclic voltammetry (CV) and differential pulse voltammetry (DPV)...
January 25, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29374599/ph-triggered-charge-reversible-of-glycol-chitosan-conjugated-carboxyl-graphene-for-enhancing-photothermal-ablation-of-focal-infection
#9
Wei Qian, Chang Yan, Danfeng He, Yunzhou Yu, Long Yuan, Menglong Liu, Gaoxing Luo, Jun Deng
Subcutaneous abscesses infected by multidrug-resistant bacteria are becoming an increasing challenge to human health. To address this challenge, a surface-adaptive and biocompatible glycol chitosan conjugated carboxyl graphene (GCS-CG) is developed, which exhibits unique self-adaptive target to the acidic microenvironment of abscess (∼ pH 6.3) and no damage to the healthy tissue (pH 7.4) around the abscess. Originally, following conjugated with GCS, the absorbance of CG obviously increased in the near-infrared (NIR) region, enabling GCS-CG to generate an increment amount of heat...
January 24, 2018: Acta Biomaterialia
https://www.readbyqxmd.com/read/29363273/preparation-and-evaluation-of-effect-on-e-coli-and-s-aureus-of-radiolabeled-ampicillin-loaded-graphene-oxide-nanoflakes
#10
Fatma Yurt, Onur Alp Ersöz, Ersan Harputlu, Kasim Ocakoglu
Ampicillin is a one of effective antibiotics against Gram-positive and Gram-negative bacteria. The present study aims to label ampicillin loaded graphene oxide nanoflake (AMP-GO) with 99m Tc and evaluate of its in vitro binding to S. aureus and E. coli. Firstly, ampicillin was loaded onto graphene oxide nanoflake prepared. AMP-GO was characterized by Fourier Transform Infrared Spectroscopy (FTIR) and Scanning Electron Microscope (SEM) techniques, and the amount of loaded ampicillin onto GO was determined by UV-Vis absorption spectroscopy...
January 24, 2018: Chemical Biology & Drug Design
https://www.readbyqxmd.com/read/29321058/graphene-oxide-conjugated-with-polymers-a-study-of-culture-condition-to-determine-whether-a-bacterial-growth-stimulant-or-an-antimicrobial-agent
#11
Ping-Ching Wu, Hua-Han Chen, Shih-Yao Chen, Wen-Lung Wang, Kun-Lin Yang, Chia-Hung Huang, Hui-Fang Kao, Jui-Cheng Chang, Chih-Li Lilian Hsu, Jiu-Yao Wang, Ting-Mao Chou, Wen-Shuo Kuo
BACKGROUND: The results showed that the deciding factor is the culture medium in which the bacteria and the graphene oxide (GO) are incubated at the initial manipulation step. These findings allow better use of GO and GO-based materials more and be able to clearly apply them in the field of biomedical nanotechnology. RESULTS: To study the use of GO sheets applied in the field of biomedical nanotechnology, this study determines whether GO-based materials [GO, GO-polyoxyalkyleneamine (POAA), and GO-chitosan] stimulate or inhibit bacterial growth in detail...
January 10, 2018: Journal of Nanobiotechnology
https://www.readbyqxmd.com/read/29315841/electron-transfer-directed-antibacterial-properties-of-graphene-oxide-on-metals
#12
Sunita Panda, Tapan K Rout, Agnish Dev Prusty, Pulickel M Ajayan, Sasmita Nayak
Nanomaterials such as silver nanoparticles and graphene-based composites are known to exhibit biocidal activities. However, interactions with surrounding medium or supporting substrates can significantly influence this activity. Here, it is shown that superior antimicrobial properties of natural shellac-derived graphene oxide (GO) coatings is obtained on metallic films, such as Zn, Ni, Sn, and steel. It is also found that such activities are directly correlated to the electrical conductivity of the GO-metal systems; the higher the conductivity the better is the antibacterial activity...
January 8, 2018: Advanced Materials
https://www.readbyqxmd.com/read/29314166/ecotoxicology-of-manufactured-graphene-oxide-nanomaterials-and-derivation-of-preliminary-guideline-values-for-freshwater-environments
#13
Marijana Markovic, Anupama Kumar, Ivan Andjelkovic, Supriya Lath, Jason K Kirby, Dusan Losic, Graeme E Batley, Michael J McLaughlin
The unique physical and chemical properties of graphene-based nanomaterials (GNMs) have inspired a diverse range of scientific and industrial applications. The market value of GNMs is predicted to reach $US 1.3 billion by 2023. Common to many nanomaterials (NMs), an important and unresolved question is the environmental consequences of the increases in GNM use. The current deficiencies in studies reporting ecotoxicology data for GNMs include, differences in analytical methodologies for quantification, no standardized test guidelines, differences in morphology of GNMs, the lack of Chemical Abstract Service (CAS) numbers and the quality of the reported data...
January 4, 2018: Environmental Toxicology and Chemistry
https://www.readbyqxmd.com/read/29313522/enhanced-antibacterial-activity-of-silver-decorated-sandwich-like-mesoporous-silica-reduced-graphene-oxide-nanosheets-through-photothermal-effect
#14
Rong Liu, Xuandong Wang, Jun Ye, Ximei Xue, Fangrong Zhang, Huicong Zhang, Xuemei Hou, Xiaolong Liu, Yun Zhang
Drug resistance of bacteria has become a global health problem, as it makes the conventional antibiotics less efficient. It is urgently needed to explore novel antibacterial materials and develop effective treatment strategies to overcome the drug resistance of antibiotics. Herein, we successfully synthesized silver decorated sandwich-like mesoporous silica/reduced graphene oxide nanosheets (rGO/MSN/Ag) as novel antibacterial material through facile method. The rGO and Ag nanoparticles can be reduced in the reaction system without adding any other reductants...
January 9, 2018: Nanotechnology
https://www.readbyqxmd.com/read/29273994/effects-of-low-level-engineered-nanoparticles-on-the-quorum-sensing-of-pseudomonas-aeruginosa-pao1
#15
Na Li, Lijia Wang, Huicong Yan, Meizhen Wang, Dongsheng Shen, Jun Yin, Jiali Shentu
The toxicity of engineered nanoparticles (ENPs) on bacteria has aroused much interest. However, few studies have focused on the effects of low-level ENPs on bacterial group behaviors that are regulated by quorum sensing (QS). Herein, we investigated the effects of nine ENPs (Ag, Fe, ZnO, TiO2, SiO2, Fe2O3, single-wall carbon nanotubes (SWCNTs), graphene oxide (GO), and C60) on QS in Pseudomonas aeruginosa PAOl. An ENP concentration of 100 μg L-1 did not impair bacterial growth. However, concentrations of 100 μg L-1 of Ag and GO ENPs induced significant increases in 3OC12-HSL in the culture and significantly promoted protease production and biofilm formation of PAO1...
December 23, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/29240395/narrow-band-gap-conjugated-polyelectrolytes
#16
Qiuhong Cui, Guillermo C Bazan
Two essential structural elements define a class of materials called conjugated polyelectrolytes (CPEs). The first is a polymer framework with an electronically delocalized, π-conjugated structure. This component allows one to adjust desirable optical and electronic properties, for example the range of wavelengths absorbed, emission quantum yields, electron affinity, and ionization potential. The second defining feature is the presence of ionic functionalities, which are usually linked via tethers that can modulate the distance of the charged groups relative to the backbone...
December 14, 2017: Accounts of Chemical Research
https://www.readbyqxmd.com/read/29235842/mussel-inspired-synthesis-of-nir-responsive-and-biocompatible-ag-graphene-2d-nano-agents-for-versatile-bacterial-disinfections
#17
Xin Fan, Fan Yang, Chuanxiong Nie, Ye Yang, Haifeng Ji, Chao He, Chong Cheng, Changsheng Zhao
Pathogenic bacterial infection has been becoming as a global threat towards people's health, especially the massive usage of antibiotics due to the lack of antibacterial agents with less side-effects. Developing new nano-agents to fight pathogenic bacteria has provided enormous new possibilities in the treatment of bacterial infections, such as the graphene based 2D antibacterial nano-agents with different bacterial inhibition capabilities; however, mussel-inspired design of NIR-responsive and biocompatible Ag-graphene nano-agents possessed with efficient and versatile bacterial disinfection activities have rarely been reported...
December 13, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29220744/sensitive-detection-of-pyoverdine-with-an-electrochemical-sensor-based-on-electrochemically-generated-graphene-functionalized-with-gold-nanoparticles
#18
Islem Gandouzi, Mihaela Tertis, Andreea Cernat, Amina Bakhrouf, Maria Coros, Stela Pruneanu, Cecilia Cristea
The design and development of an electrochemical sensor for the sensitive and selective determination of pyoverdine, a virulence factor secreted by Pseudomonas aeruginosa, bacteria involved in nosocomial infections is presented in this work. The presence of pyoverdine in water and body fluids samples can be directly linked to the presence of the Pseudomonas bacteria, thus being a nontoxic and low cost marker for the detection of water pollution as well as for the biological contamination of other media. The sensor was elaborated using layer-by-layer technique for the deposition of a graphene‑gold nanoparticles composite film on the graphite-based screen printed electrode, from aqueous suspension...
December 5, 2017: Bioelectrochemistry
https://www.readbyqxmd.com/read/29218826/poly-lactic-acid-composites-based-on-graphene-oxide-particles-with-antibacterial-behavior-enhanced-by-electrical-stimulus-and-biocompatibility
#19
P Arriagada, H Palza, P Palma, M Flores, P Caviedes
Poly(lactic acid) (PLA) is a biodegradable and biocompatible polyester widely used in biomedical applications. Unfortunately, this biomaterial suffers from some shortcomings related with the absence of both bioactivity and antibacterial capacity. In this work, composites of PLA with either graphene oxide (GO) or thermally reduced graphene oxide (TrGO) were prepared by melt mixing to overcome these limitations. PLA composites with both GO and TrGO inhibited the attachment and proliferation of E. coli and S. aureus bacteria depending on the kind and amount of filler...
December 8, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/29216517/go-agcl-ag-nanocomposites-with-enhanced-visible-light-driven-catalytic-properties-for-antibacterial-and-biofilm-disrupting-applications
#20
Xinhuan Wang, Qiusen Han, Ning Yu, Tian Wang, Chen Wang, Rong Yang
Nanomaterials with visible light-driven photocatalytic activity have attracted much attention due to their excellent abilities in degradation of various organic pollutants as well as inactivating bacteria. Herein, graphene oxide (GO) enwrapped silver chloride/silver (AgCl/Ag) nanocomposites with high visible light absorption were designed and fabricated as efficient antibacterial agents. AgCl NPs were synthesized in the presence of GO first and Ag NPs were coated on AgCl surface by heat reduction to form GO-AgCl/Ag nanocomposites...
November 26, 2017: Colloids and Surfaces. B, Biointerfaces
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