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Graphene bacteria

Mahmoud A Hussein, Reda M El-Shishtawy, Khalid A Alamry, Abdullah M Asiri, Saleh A Mohamed
Pathogens, such as Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus), contaminate water resources and are the main causes of diseases, not just waterborne diseases. The present study aims to fabricate hybrid materials composed of polyaniline (PANI), graphene nanosheets (GNs), and carbon nanotubes (CNTs) and utilize the materials for water disinfection. Thus, a new class of hybrid nanocomposites (PANI/GN/CNTsa-e ) was fabricated under ultrasonic conditions following a well-known in situ oxidative polymerization technique in an aqueous acidic solution...
April 19, 2018: Environmental Technology
Ruiwen Wang, Mei Yan, Huidong Li, Lu Zhang, Benqi Peng, Jinzhi Sun, Da Liu, Shaoqin Liu
Microbial fuel cells (MFCs) have received great attention worldwide due to their potential in recovering electrical energy from waste and inexhaustible biomass. Unfortunately, the difficulty of achieving the high power, especially in real samples, remains a bottleneck for their practical applications. Herein, FeS2 nanoparticles decorated graphene is fabricated via a simple hydrothermal reaction. The FeS2 nanoparticles decorated graphene anode not only benefits bacterial adhesion and enrichment of electrochemically active Geobacter species on the electrode surface but also promotes efficient extracellular electron transfer, thus giving rise to a fast start-up time of 2 d, an unprecedented power density of 3220 mW m-2 and a remarkable current density of 3...
April 17, 2018: Advanced Materials
Mara Di Giulio, Romina Zappacosta, Silvia Di Lodovico, Emanuela Di Campli, Gabriella Siani, Antonella Fontana, Luigina Cellini
Chronic wounds represent an increasing problem worldwide. Graphene oxide (GO) has been reported to exhibit strong antibacterial activity towards both Gram-positive and Gram-negative bacteria. The aim of this work was to investigate the in vitro antimicrobial and antibiofilm efficacy of GO against wound pathogens. Staphylococcus aureus PECHA 10, Pseudomonas aeruginosa PECHA 4 and Candida albicans X3 clinical isolates were incubated with 50 mg/L of GO for 2 and 24 h to evaluate the antimicrobial effect. Optical and Atomic force microscopy images were performed to visualize the GO effect on microbial cells...
April 16, 2018: Antimicrobial Agents and Chemotherapy
Xinyu Meng, Houyu Wang, Na Chen, Pan Ding, Huayi Shi, Xia Zhai, Yuanyuan Su, Yao He
There currently exists increasing concerns on the development of a kind of high-performance SERS platform, which is suitable for sensing applications ranging from molecular to cellular (e.g., bacteria) level. Herein, we develop a novel kind of universal SERS chip, made of graphene (G)-silver nanoparticles (AgNPs)-silicon (Si) sandwich nanohybrids (G@AgNPs@Si), in which AgNPs are in situ grown on silicon wafer through hydrofluoric acid-etching assisted chemical reduction, followed by coating with a single-layer graphene via polymer-protective etching method...
April 2, 2018: Analytical Chemistry
Yi Ning, Li Zou, Qiang Gao, Jue Hu, Fangguo Lu
The authors describe a method for the fluorometric determination of methicillin-resistant Staphylococcus aureus (MRSA) by exploiting target-triggered chain reactions and deoxyribonuclease I (DNase I)-aided target recycling. It is making use of a carboxy-fluorescein (FAM)-labeled single-stranded probe containing two sections. One is complementary to the 5' terminus of the target, while the 3' terminus of the other target is adsorbed on the surface of graphene oxide (GO) via π-stacking interactions without the target (16S rRNA)...
February 17, 2018: Mikrochimica Acta
Raja Chinnappan, Saleh AlAmer, Shimaa Eissa, Anas Abdel Rahamn, Khalid M Abu Salah, Mohammed Zourob
The work describes a fluorescence-based study for mapping the highest affinity truncated aptamer from the full length sequence and its integration in a graphene oxide platform for the detection of Salmonella enteriditis. To identify the best truncated sequence, molecular beacons and a displacement assay design are applied. In the fluorescence displacement assay, the truncated aptamer was hybridized with fluorescein and quencher-labeled complementary sequences to form a fluorescence/quencher pair. In the presence of S...
December 18, 2017: Mikrochimica Acta
Bhawana Thakur, Guihua Zhou, Jingbo Chang, Haihui Pu, Bing Jin, Xiaoyu Sui, Xiaochen Yuan, Ching-Hong Yang, Matthew Magruder, Junhong Chen
Contamination of surface and drinking water due to the presence of Escherichia coli bacteria is a major cause of water-borne disease outbreak. To address unmet challenges for practical pathogen detection in contaminated samples, we report fabrication of thermally reduced graphene oxide-based field-effect transistor (rGO FET) passivated with an ultrathin layer of Al2 O3 for real-time detection of E. coli bacteria. The sensor could detect a single E. coli cell within 50 s in a 1 µL sample volume. The ultrathin layer of Al2 O3 acted as a barrier between rGO and potential interferents present in the sample...
March 9, 2018: Biosensors & Bioelectronics
Chao Guo, Yatao Wang, Yulong Luo, Xiaoguo Chen, Yaolin Lin, Xiaoying Liu
With the widespread application of graphene oxide (GO), it would be inevitably released into wastewater treatment plants (WWTPs) and get involved in the biochemical process. So far, there are controversies on the effects of low GO concentration (0.05-0.1 g/L) on the nitrogen removal process. Therefore, this study essentially investigates any potential effects of GO on wastewater microbial communities functions. In present study, the nitrifying and denitrifying batch tests were introduced to investigate the influence of 0...
March 19, 2018: Ecotoxicology and Environmental Safety
Li Sun, Zhuanjun Yan, Youxin Duan, Junyan Zhang, Bin Liu
OBJECTIVE: The aim of this study was to improve the mechanical properties, wear resistance and antibacterial properties of conventional glass ionomer cements (GICs) by fluorinated graphene (FG), under the premise of not influencing their solubility and fluoride ion releasing property. MATERIALS AND METHODS: FG with bright white color was prepared using graphene oxide by a hydrothermal reaction. Experimental modified GICs was prepared by adding FG to the traditional GICs powder with four different weight ratios (0...
March 19, 2018: Dental Materials: Official Publication of the Academy of Dental Materials
Fatemeh Kiani, Negar Ashari Astani, Reza Rahighi, Ahmad Tayyebi, Meysam Tayebi, Jafar Khezri, Ehsan Hashemi, Ursula Rothlisberger, Abdolreza Simchi
In the present work, the effect of graphene oxide (GO) nanosheets on the antibacterial activity of CuO nanowire arrays under visible light irradiation is shown. A combined thermal oxidation/electrophoretic deposition technique was employed to prepare three-dimensional networks of graphene oxide nanosheets hybridized with vertically aligned CuO nanowires. With the help of standard antibacterial assays and X-ray photoelectron spectroscopy, it is shown that the light-activated antibacterial response of the hybrid material against gram-negative Escherichia coli is significantly improved as the oxide functional groups of the GO nanosheets are reduced...
March 7, 2018: Journal of Colloid and Interface Science
Katherine D Hills, Daniela A Oliveira, Nicholas D Cavallaro, Carmen L Gomes, Eric S McLamore
We demonstrate a sensing mechanism for rapid detection of Listeria monocytogenes in food samples using the actuation of chitosan-aptamer nanobrush borders. The bio-inspired soft material and sensing strategy mimic natural symbiotic systems, where low levels of bacteria are selectively captured from complex matrices. To engineer this biomimetic system, we first develop reduced graphene oxide/nanoplatinum (rGO-nPt) electrodes, and characterize the fundamental electrochemical behavior in the presence and absence of chitosan nanobrushes during actuation (pH-stimulated osmotic swelling)...
March 15, 2018: Analyst
Truong Thi Tuong Vi, Selvaraj Rajesh Kumar, Bishakh Rout, Chi-Hsien Liu, Chak-Bor Wong, Chia-Wei Chang, Chien-Hao Chen, Dave W Chen, Shingjiang Jessie Lue
In this work, silver nanoparticles (Ag NPs) were decorated on thiol (-SH) grafted graphene oxide (GO) layers to investigate the antibacterial activities in Gram-positive bacteria ( Staphylococcus aureus ) and Gram-negative bacteria ( Pseudomonas aeruginosa ). The quasi-spherical, nano-sized Ag NPs were attached to the GO surface layers, as confirmed by using field emission scanning electron microscopy (FESEM) and transmission electron microscopy (TEM), respectively. The average size of GO-Ag nanocomposites was significantly reduced (327 nm) from those of pristine GO (962 nm) while the average size of loaded Ag NPs was significantly smaller than the Ag NPs without GO...
March 14, 2018: Nanomaterials
Jaroslav Jira, Bohuslav Rezek, Vitezslav Kriha, Anna Artemenko, Iva Matolínová, Viera Skakalova, Pavla Stenclova, Alexander Kromka
Nanodiamonds (NDs) and graphene oxide (GO) are modern carbon-based nanomaterials with promising features for the inhibition of microorganism growth ability. Here we compare the effects of nanodiamond and graphene oxide in both annealed (oxidized) and reduced (hydrogenated) forms in two types of cultivation media-Luria-Bertani (LB) and Mueller-Hinton (MH) broths. The comparison shows that the number of colony forming unit (CFU) of Escherichia coli is significantly lowered (45%) by all the nanomaterials in LB medium for at least 24 h against control...
March 1, 2018: Nanomaterials
Muhammad Junaid Afzal, Erum Pervaiz, Sarah Farrukh, Tahir Ahmed, Zhang Bingxue, Minghui Yang
Highly integrated nanocomposite of Graphene oxide (GO) and its derivatives with metal oxides is essential for enhanced performance for various applications. Tuning the morphology is an important aspect during nanomaterials synthesis; this has an amplifying influence upon physicochemical properties of advanced functional materials. In this research work, Graphene oxide/TiO2 nanotube composites have been successfully synthesized via alkaline hydrothermal treatment method by augmenting GO layers with two different phases of TiO2 (anatase and rutile) nanoparticles, followed by the hydrothermal treatment that also have caused reduction of GO to reduced GO (RGO)...
March 1, 2018: Environmental Technology
Nuno F F Moreira, Carlos Narciso-da-Rocha, M Inmaculada Polo-López, Luisa M Pastrana-Martínez, Joaquim L Faria, Célia M Manaia, Pilar Fernández-Ibáñez, Olga C Nunes, Adrián M T Silva
Solar-driven advanced oxidation processes were studied in a pilot-scale photoreactor, as tertiary treatments of effluents from an urban wastewater treatment plant. Solar-H2 O2 , heterogeneous photocatalysis (with and/or without the addition of H2 O2 and employing three different photocatalysts) and the photo-Fenton process were investigated. Chemical (sulfamethoxazole, carbamazepine, and diclofenac) and biological contaminants (faecal contamination indicators, their antibiotic resistant counterparts, 16S rRNA and antibiotic resistance genes), as well as the whole bacterial community, were characterized...
February 3, 2018: Water Research
Muhammad Usman Farid, Sanghyun Jeong, Dong Han Seo, Rafay Ahmed, Condon Lau, Nirmal Kumar Gali, Zhi Ning, Alicia Kyoungjin An
While the cytotoxicity of graphene oxide (GO) has been well established, its bactericidal mechanism, however, has yet to be elucidated to advance GO-based biomedical and environmental applications. In an attempt to better understand the bactericidal action of GO, herein we studied the interactions of GO with Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus cells using physical techniques and chemical probes, respectively. In particular, a novel laser-induced breakdown spectroscopy (LIBS) based elemental fingerprint analysis revealed notable differences between viable and non-viable cells based on the difference in the concentration of trace inorganic elements in complex bacterial systems, which reflect cellular membrane integrity...
February 20, 2018: Nanoscale
Tian-Shun Song, Wei-Min Tan, Jingjing Xie
In this paper, we developed an environmental friendly, cost effective, simple and green approach to reduce graphene oxide (GO) by a sulfate-reducing bacterium Desulfovibrio desulfuricans. The D. desulfuricans reduces exfoliated GO to reduced graphene oxide (rGO) at 25 °C in an aqueous solution without any toxic and environmentally harmful reducing agents. The rGO was characterized with X-ray Diffraction, Fourier Transform Infrared Spectroscopy, Scanning Electron Microscopy, Transmission Electron Microscope, X-ray Photoelectron Spectroscopy and Raman Spectroscopy...
August 1, 2018: Journal of Nanoscience and Nanotechnology
Monika Mortimer, Naresh Devarajan, Dong Li, Patricia A Holden
Carbonaceous and boron nitride (BN) nanomaterials have similar applications and hydrophobic properties suggesting common release pathways and exposure to bacteria. While high nanomaterial concentrations can be bactericidal or growth-inhibitory, little is known regarding bacterial transcriptional responses to non-growth-inhibitory nanomaterial concentrations. Here, using one strain of Pseudomonas aeruginosa-a clinically and environmentally important bacterial taxon-we analyzed the comparative transcriptomic response to carbonaceous or BN nanomaterials...
February 19, 2018: ACS Nano
Huixuan Zhang, Xiaoyong Deng, Qiuling Ma, Yuqi Cui, Xiuwen Cheng, Mingzheng Xie, Xiaoli Li, Qingfeng Cheng
In this study, silver decorated graphene oxide (Ag/GO) composite was fabricated through a reduction process in the presence of potassium borohydride solution. Subsequently, physicochemical properties of the resulting Ag/GO composite were studied by scanning electron microscope, X-ray diffraction, Raman spectra, Fourier transformation infrared spectroscopy and UV-visible diffuse reflectance spectrum. Results indicated that Ag species existed in the form of Ag0, which greatly facilitated the visible light absorbance ability...
April 1, 2018: Journal of Nanoscience and Nanotechnology
Guanhui Gao, Lingwei Wang, Peng Yang, Liang Huang, Xiaobin Xie, Jingsong Wu, Chen Qiu, Lintao Cai
We developed a facile and green bio-inspired strategy to fabricate silver nano-particles growth in-situ on different scaffolds materials, building novel heterostructures for promoting their antibacterial activities and durability. Firstly, fluorinated graphene oxide (FGO) nanosheets, layered molybdenum disulfide (MoS2), and layered tungsten disulfide (WS2) were exfoliated by chemical liquid with intense sonication. And silicon dioxide (SiO2) nano-spheres were prepared via wet chemical method. Then, silver nanoparticles were grown onto those surfaces of layered nanosheets and nano-spheres, hybridizing three dimensional hetero-architectures...
June 1, 2018: Journal of Nanoscience and Nanotechnology
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