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Ayokunle O Ademosun, Adeniyi A Adebayo, Ganiyu Oboh
Background Citrus peels have been reported useful in folk medicine for the management of cardiovascular diseases, but there is dearth of information on the possible mechanisms for their therapeutic action. The aim of this study was to investigate the effect of methanolic extracts from some citrus [lime (Citrus limon), tangerine (Citrus reticulata), shaddock (Citrus maxima)] peels on some enzymes relevant to the management of cardiovascular diseases [monoamine oxidase (MAO), phosphodiesterase-5 (PDE-5) and angiotensin-1-converting enzyme (ACE)]...
August 11, 2018: Journal of Complementary & Integrative Medicine
Guliang Yang, Shiming Li, Yiwen Yang, Li Yuan, Peilei Wang, Hui Zhao, Chi-Tang Ho, Chi-Chen Lin
Psoriasis is a chronic and benign proliferative skin disease. Flavonoids in chenpi (aged tangerine peel) from tangerine ( Citrus reticulate Blanco), such as nobiletin (Nob), tangeretin, and 5-hydroxy-6,7,8,3',4'-pentamethoxyflavone (5-HPMF), possess anti-inflammation and regulation of immune activity among others. In this study, psoriasis-like skin lesions were induced by 12- O-tetradecanoylphorbol-13-acetate (TPA), and the preventive effect of Nob and 5-HPMF on psoriasis-like skin lesions was evaluated. Results showed that skin lesions were dramatically reduced by Nob and 5-HPMF...
August 8, 2018: Journal of Agricultural and Food Chemistry
Antoni Delpino-Rius, Alexis Marsol-Vall, Jordi Eras, Montse Llovera, Miguel Ángel Cubero, Mercè Balcells, Ramon Canela-Garayoa
Here we analysed the content of primary and secondary metabolites in nine types of industrially processed fibres derived from the juice industry. Specifically, we examined fibre from: apple, peach, and pear, as non-citrus fruits; the peel and flesh of orange and tangerine, and lemon flesh, as citrus fruits; and carrot, as vegetable. Regarding primary metabolites, the sugar content ranged from 21.6 mg/g in lemon to 290 mg/g in orange peel and lower mass organic acid content ranged from 25.0 mg/g in pear to 250 mg/g in lemon...
September 2018: Food Research International
Muhammad Arshad, Muhammad Irfan Ullah, Jawwad A Qureshi, Muhammad Afzal
Feeding damage by arthropods exposes plants to pathogens and interferes with plant physiological processes. Feeding by the citrus leafminer (CLM), Phyllocnistis citrella Stainton (Lepidoptera: Gracillariidae), larva exposes leaf cuticle to the causal pathogens of the citrus canker disease. We investigated the impact of larval feeding on photosynthetic rate (Pn), CO2 exchange rate (Ci), and H2O exchange rate (Wi) in eight citrus cultivars [Citrus mandarins (Kinnow, Seedless Kinnow, Feutrell's early), Citrus sinensis (Succari, Salustiana), Citrus tangerines (Fairchild), Citrus limon (China lemon), and Citrus paradisi Macfad (Grapefruit)] under natural conditions in comparison with control plants protected from larval feeding...
June 6, 2018: Journal of Economic Entomology
Mingshuang Wang, Xiao Yang, Ruoxin Ruan, Huilan Fu, Hongye Li
The COP9 signalosome (CSN) is a highly conserved protein complex involved in the ubiquitin-proteasome system. Its metalloisopeptidase activity resides in subunit 5 (CSN5). Functions of csn5 in phytopathogenic fungi are poorly understood. Here, we knocked out the csn5 ortholog ( Aacsn5 ) in the tangerine pathotype of Alternaria alternata . The Δ Aacsn5 mutant showed a moderately reduced growth rate compared to the wildtype strain and was unable to produce conidia. The growth of Δ Aacsn5 mutant was not affected in response to oxidative and osmotic stresses...
2018: Frontiers in Microbiology
Xiaowen Dou, Lei Zhang, Congmin Liu, Qian Li, Jiaoyang Luo, Meihua Yang
A new kind of labelled antibody was synthesized by modifying gold nanoparticles (AuNPs) with the fluorophore rhodamine B isothiocyanate (RBITC) and a secondary antibody (IgG). The conjugate thus obtained was used in a competitive sandwich assay with a turn-on signal change. It was designed to detect the organophosphorus pesticide chlorpyrifos. The fluorescence of the RBITC-labeled gold immunoprobe with emission at 575 nm and excitation at 556 nm is almost completely quenched. If, however, cysteamine is added, the fluorophore is released from the labeled secondary antibody and fluorescence increases in accordance with the quantity of secondary antibody bound to the sandwich...
December 8, 2017: Mikrochimica Acta
Francesca Garganese, Antonio Ippolito, Valentina di Rienzo, Concetta Lotti, Cinzia Montemurro, Simona M Sanzani
BACKGROUND: Alternaria brown spot is one of the most important diseases of tangerines and their hybrids worldwide. To set up effective control strategy, the accurate detection and identification of the species responsible for the diseases is crucial. However, characterization based on morphology and/or multilocus genetic approaches is time consuming, requires great expertise and sometimes is not conclusive. Therefore, the set-up of a rapid and efficient DNA-based assay might be of paramount importance...
March 5, 2018: Journal of the Science of Food and Agriculture
Jinlong Wu, Wenjun Deng, Dingbo Lin, Xiuxin Deng, Zhaocheng Ma
The inherent allergens of citrus fruits, such as Cit s 1, Cit s 2, Cit s 3 can cause allergic reactions. A better understanding of the genetic factors (cultivar to cultivar) affecting the allergenic potential of citrus fruits would be beneficial for further identification of hypoallergenic genotypes. In the present study, an immunoblotting quantification approach was adopted to assess the potential allergenicity of 21 citrus cultivars, including nine subgroups (tangerine, satsuma, orange, pummelo, grapefruit, lemon, kumquat, tangor, and tangelo)...
February 28, 2018: Journal of Agricultural and Food Chemistry
Javed Nawab, Saad Farooqi, Wang Xiaoping, Sardar Khan, Asad Khan
Food safety is a major concern worldwide and human beings are frequently exposed to potentially toxic metals (PTMs) through consumption of vegetables, fruits, and cereal crops grown in contaminated areas. The present study investigates the concentrations of PTMs such as chromium (Cr), nickel (Ni), zinc (Zn), arsenic (As), cadmium (Cd), and lead (Pb) in the foodstuffs (fruits, vegetables, and cereals) collected from different markets of Khyber Pakhtunkhwa, Pakistan. Samples of fruits (banana, tangerine, apple, and guava), vegetables (tomato, onion, potato, pea, and lady finger), and cereals (rice, kidney beans, and chick peas) were acid-extracted and analyzed using ICP-MS...
February 2018: Environmental Science and Pollution Research International
Yolanda Picó, Mohamed A El-Sheikh, Ahmed H Alfarhan, Damià Barceló
The occurrence of pesticide residues in fruits was determined by a target method for 62 analytes and a wide scope screening method against a database of 500 pesticides. Limits of quantification (LOQs) were from 0.5 to 6.3 μg kg-1 for the target method and from 0.5 to 119 μg kg-1 for the non-target. Thirty samples: dates, apples, oranges, tangerines, lemons and grapefruits were selected because their high consumption, except lemons and grapefruits that were to cover all citrus fruits. Using the target method, 15 compounds (mostly insecticides and fungicides) out of 62 pesticides (organophosphorus, carbamates, pyrethroids, chloroacetanilides, triazines, triazoles, imidazoles, etc...
January 2018: Food and Chemical Toxicology
N P Dias, D E Nava, M S Garcia, F F Silva, R A Valgas
Fruit flies (Diptera: Tephritidae) represent a threat to fruit growing worldwide, mainly the citrus culture, however, biological studies show that fruit flies are not perfectly adapted to this host. This study investigated oviposition of Anastrepha fraterculus (Wiedemann, 1830) and Ceratitis capitata (Wiedemann, 1824) and its relation with the pericarp of citrus fruits. We evaluated the relationship between depth of oviposition of A. fraterculus and C. capitata and epicarp thickness of orange [Citrus sinensis (L...
August 2018: Brazilian Journal of Biology, Revista Brasleira de Biologia
Li-Ping Chen, Yong-Min Cai, Jian-Sheng Li
To explore the medication rules of famous veteran traditional Chinese medicine (TCM) doctors in treatment of chronic bronchitis, a structured medical record database for famous veteran TCM doctors in modern clinical books was established. First, Lantern 3.1.2(Kongmin light) implicit structure analysis software was used to build an implicit structure model and make an implicit interpretation. Then, SAS 9.1 was adopted to mine herb-herb, herb-symptom and herb-syndrome association rules. Through the mining, 1 274 commonly used herbs for chronic bronchitis were found, including liquorice, bitter almond, pinellia, dried tangerine or orange peel, poria cocos...
April 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
Fran Nekvapil, Ioana Brezestean, Daniel Barchewitz, Branko Glamuzina, Vasile Chiş, Simona Cintă Pinzaru
The freshness of citrus fruits commonly available in the market was non-destructively assessed by Raman spectroscopy. Intact clementine, mandarin and tangerine species were characterised concerning their carotenoids skin Raman signalling in a time course from the moment they were acquired as fresh stock, supplying the market, to the physical degradation, when they were no longer attractive to consumers. The freshness was found to strongly correlate to the peel Raman signal collected from the same area of the intact fruits in a time course of a maximum of 20days...
March 1, 2018: Food Chemistry
Chang-Lin Lai, Hong Wu, Gen-Jin Ni
Guangdong Pericarpium Citri Reticulatae is the traditional Chinese herbal medicine, and has been used for a long history. The authentic drug name Guangdong Pericarpium Citri Reticulatae was rare in the Song Dynasty literature, but from the textual research of the authors, it was otherwise nicknamed as "real orange peel" or "real tangerine peel". These two names were ahead of the origin of Guangdong Pericarpium Citri Reticulatae. Some scholars believe that the name Guangdong Pericarpium Citri Reticulatae was from the Ming Dynasty Dr...
February 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
Morgan J Cichon, Ken M Riedl, Lei Wan, Jennifer M Thomas-Ahner, David M Francis, Steven K Clinton, Steven J Schwartz
SCOPE: Diets rich in tomato products are associated with a reduced risk of various chronic disease processes. The carotenoid lycopene is most intensely studied as the bioactive mediating health effects, yet tomatoes contain an array of phytochemicals. An untargeted metabolomics study is conducted on blood plasma to identify novel markers of tomato consumption absorbed from the diet and released into the bloodstream in mice. METHODS AND RESULTS: Male mice are fed a control AIN-93G diet or the same diet supplemented with 0...
December 2017: Molecular Nutrition & Food Research
Jessica L Cooperstone, Kathleen L Tober, Ken M Riedl, Matthew D Teegarden, Morgan J Cichon, David M Francis, Steven J Schwartz, Tatiana M Oberyszyn
Prolonged tomato consumption can mitigate ultraviolet (UV) light induced sunburn via unknown mechanisms. Dietary carotenoids distributed to skin are hypothesized to protect skin against UV-induced damage, although other phytochemicals may play a role. We hypothesize that tomato consumption would protect against skin cancer. SKH-1 hairless and immunocompetent mice (n = 180) were fed AIN-93G or AIN-93G + 10% tangerine or red tomato powder for 35 weeks. From weeks 11-20, mice (n = 120) were exposed to 2240 J/m(2) UV-B light, 3x/week, and tumors were tracked weekly...
July 11, 2017: Scientific Reports
Yue Wang, Jing Qian, Jinping Cao, Dengliang Wang, Chunrong Liu, Rongxi Yang, Xian Li, Chongde Sun
Citrus ( Citrus reticulate Blanco) is one of the most commonly consumed and widely distributed fruit in the world, which is possessing extensive bioactivities. Present study aimed to fully understand the flavonoids compositions, antioxidant capacities and in vitro anticancer abilities of different citrus resources. Citrus fruits of 35 varieties belonging to 5 types (pummelos, oranges, tangerines, mandarins and hybrids) were collected. Combining li quid chromatography combined with electrospray ionization mass spectrometry (LC-ESI-MS/MS) and ultra-performance liquid chromatography combined with diode array detector (UPLC-DAD), a total of 39 flavonoid compounds were identified, including 4 flavones, 9 flavanones and 26 polymethoxylated flavonoids (PMFs)...
July 5, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Matthias Bader, Tatjana Lang, Roman Lang, Thomas Hofmann
To validate the suitability of synephrine, known to be a highly abundant alkaloid in oranges, as a dietary biomarker for orange consumption, a highly sensitive and robust stable isotope dilution analysis (SIDA) as well as an ECHO method, using the analyte itself as a pseudointernal standard injected into the analysis run to provide an "echo peak" of the analyte, was developed to quantitate synephrine by liquid chromatography-tandem mass spectrometry (LC-MS/MS) in citrus juices and human urine before and after the ingestion of orange juice...
June 14, 2017: Journal of Agricultural and Food Chemistry
Haipeng Zhang, Yunxia Xie, Cuihua Liu, Shilin Chen, Shuangshuang Hu, Zongzhou Xie, Xiuxin Deng, Juan Xu
The volatile profiles of fruit peels and juice sacs from 108 citrus accessions representing seven species were analyzed. Using GC-MS 162 and 107 compounds were determined in the peels and juice sacs, respectively. In the peels, monoterpene alcohols were accumulated in loose-skin mandarins; clementine tangerines and papedas were rich in sesquiterpene alcohols, sesquiterpenes, monoterpene alcohols and monoterpene aldehydes. β-pinene and sabinene were specifically accumulated in 4 of 5 lemon germplasms. Furthermore, concentrations of 34 distinctive compounds were selected to best represent the volatile profiles of seven species for HCA analysis, and the clustering results were in agreement with classic citrus taxonomy...
September 1, 2017: Food Chemistry
Morgan J Cichon, Ken M Riedl, Steven J Schwartz
Juices from the traditional red tomato and a unique tangerine tomato variety are being investigated as health promoting foods in human clinical trials. However, it is unknown how the tangerine and red tomato juices differ in biologically relevant phytochemicals beyond carotenoids. Here liquid-chromatography high-resolution mass spectrometry metabolomics was used to evaluate broadly the similarities and differences in carotenoids and other phytochemicals between red and tangerine tomato juices intended for clinical interventions...
August 1, 2017: Food Chemistry
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