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https://www.readbyqxmd.com/read/30334828/cutaneous-leishmaniasis-contribution-of-routine-histopathology-in-unexpected-encounters
#1
Jerome B Taxy, Harry M Goldin, Sara Dickie, Thomas Cibull
The ulcerated papules of cutaneous leishmaniasis represent an unusual form of granulomatous dermatitis caused by Leishmania species, a protozoan transmitted by sandfly bites. As the disease is not native to North America, clinical suspicion is partially based on a compatible travel history and may result in a biopsy. The key role of standard morphology is the identification of Leishmania organisms, supplemented by Giemsa and/or CD1a immunostaining. Histologically, the organism may be confused with Histoplasma species, which is resolved by staining with methenamine silver or PAS...
October 16, 2018: American Journal of Surgical Pathology
https://www.readbyqxmd.com/read/30246397/diagnostic-values-of-koh-examination-histological-examination-and-culture-for-onychomycosis-a-latent-class-analysis
#2
Bilge Fettahl Oğlu Karaman, Arbil Açıkalın, İlker Ünal, Varol L Aksungur
BACKGROUND: In the absence of a real gold standard, comparative studies are still done on diagnostic methods for onychomycosis. There are only a few attempts using latent class analysis to determine the value of polymerase chain reaction in comparison to conventional methods. We aimed to determine the value of histological examination in such a way for the diagnosis of onychomycosis. METHODS: Potassium hydroxide mount (KOH), culture and histological examination with periodic acid-Schiff (PAS), and Gomori's methenamine silver (GMS) stains were done in 106 patients having clinically suspected toenail onychomycosis...
September 23, 2018: International Journal of Dermatology
https://www.readbyqxmd.com/read/30227609/do-pentraxins-bind-to-fungi-in-invasive-human-gastrointestinal-candidiasis
#3
Umamaheshwari Golconda, Richard E Sobonya, Stephen A Klotz
Tissue from 13 autopsy cases with invasive gastrointestinal candidiasis was studied for the binding of the pentraxins, C-reactive protein (CRP), pentraxin 3 (PTX3), and serum amyloid P component (SAP) to fungal surfaces. Invasive candidal infection was demonstrated using a hematoxylin and eosin stain and a Gomori methenamine silver stain (GMS). Immunohistochemistry was performed with CRP and PTX3 monoclonal antibodies and did not demonstrate CRP or PTX3 bound to fungi (0 of 13 cases), although CRP was extensively deposited on human tissue...
September 17, 2018: Journal of Fungi (Basel, Switzerland)
https://www.readbyqxmd.com/read/30193562/pathology-of-neonatal-non-albicans-candidiasis-autopsy-study-and-literature-review
#4
Claire Hemedez, Elizabeth Trail-Burns, Quanfu Mao, Sharon Chu, Sunil K Shaw, Joseph M Bliss, Monique E De Paepe
Introduction/Objectives Non- albicans Candida species such as Candida parapsilosis and Candida glabrata have emerged as prevalent pathogens in premature infants. The aim of this study was to systematically delineate the histopathologic findings in neonatal non- albicans candidiasis. Methods We performed a retrospective clinicopathologic analysis of extremely premature (23-28 weeks' gestation) infants diagnosed with invasive candidiasis. Archival autopsy tissues were subjected to periodic acid-Schiff, methenamine-silver and anti- Candida (immuno)histochemical stains, as well as dual anti- Candida and anti-cytokeratin or anti-CD31 immunofluorescence assays...
September 7, 2018: Pediatric and Developmental Pathology
https://www.readbyqxmd.com/read/30138986/-epidemiological-characteristics-of-pneumocystis-jirovecii-infection-and-colonization-in-non-aids-patients
#5
S Long, X D Mu, C Zhang, L Su, P Jia, P N Zhou, G F Wang
Objective: To analyze the epidemiological characteristics of Pneumocystis jirovecii infection and colonization in non-AIDS patients. Methods: From January 2010 to December 2017, bronchoalveolar lavage fluid (BALF) was detected by Grocott's methenamine silver (GMS) staining and real-time fluorescence quantitative PCR (qPCR) in non-AIDS patients with bronchoscopic alveolar lavage at Peking University First Hospital. At the same time, Pneumocystis jirovecii was detected in the environment of the hospital. Results: Within 8 years, Pneumocystis jirovecii were detected in BALF of a total of 1 407 non-AIDS patients...
August 14, 2018: Zhonghua Yi Xue za Zhi [Chinese medical journal]
https://www.readbyqxmd.com/read/30054925/panniculitis-in-a-patient-with-pathologic-complete-response-to-talimogene-laherparepvec-treatment-for-recurrent-in-transit-melanoma
#6
Thomas H Long, Michi M Shinohara, Zsolt B Argenyi, John A Thompson, Jennifer M Gardner
Talimogene laherparepvec (T-VEC) is a novel intralesional oncolytic genetically modified herpes simplex virus type 1 vector for the treatment of unresectable cutaneous, subcutaneous, and nodal melanoma. Although immunological therapies such as T-VEC offer therapeutic promise, they carry a risk of immune-related adverse events (irAEs), the full spectrum of which is incompletely understood. We report a 63-year-old previously healthy man with cutaneous melanoma of the right ankle and progressive right lower extremity in-transit metastases despite systemic therapy with immunomodulatory and molecularly targeted treatments...
July 27, 2018: Journal of Cutaneous Pathology
https://www.readbyqxmd.com/read/30021823/bronchoalveolar-lavage-fluid-cytology-in-culture-documented-invasive-pulmonary-aspergillosis-in-patients-with-hematologic-diseases-analysis-of-67-episodes
#7
A Fernández-Cruz, E Magira, S T Heo, S Evans, J Tarrand, D P Kontoyiannis
There is a paucity of studies on the yield of Gomori-methenamine-silver (GMS) staining in bronchoalveolar lavage (BAL) fluid cytology and its comparison with fluorescent dye staining for the diagnosis of invasive pulmonary aspergillosis (IPA) in patients with hematologic malignancies. To that end, we analyzed the yield of direct fungal visualization in BAL fluid cytology with GMS staining, in a series of culture-positive IPA cases in 67 patients with hematologic malignancies, and we compared the results with those of direct examination with calcofluor white staining and BAL fluid galactomannan assays, when available...
October 2018: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/30020094/epithelial-inclusions-in-gallbladder-specimens-mimic-parasite-infection-histologic-and-molecular-examination-of-reported-cystoisospora-belli-infection-in-gallbladders-of-immunocompetent-patients
#8
Eric A Swanson, Jordon K March, Frederic Clayton, Marc R Couturier, Ramir Arcega, Richard Smith, Kimberley J Evason
Recent publications have described epithelial cytoplasmic vacuoles and inclusions incidentally noted within gallbladder epithelium and concluded that they represent coccidian parasite infection, in particular, Cystoisospora belli. We identified 8 gallbladder specimens from our institution in the past 3 years in which this diagnosis was suggested or in which similar epithelial alterations were prominent. Molecular analysis was performed on the 8 gallbladder specimens and on 3 positive control specimens: small bowel biopsies from acquired immunodeficiency syndrome patients with diarrhea...
October 2018: American Journal of Surgical Pathology
https://www.readbyqxmd.com/read/29982578/changing-practice-patterns-for-cytotechnologists-a-comparative-analysis-of-data-from-the-2009-and-2015-ascp-boc-practice-analysis-surveys
#9
Janie Roberson, Asma M Ali, Jennifer Clark, Isam Eltoum, David Ritter, Ryan Soles
Background: The American Society for Clinical Pathology (ASCP) Board of Certification (BOC) surveys US certified cytotechnologists (CTs) at approximately 5-year intervals to gain information about current practice patterns. Although the purpose of this survey is to inform valid content development for the BOC CT examination, comparative longitudinal analysis of the survey data provides information about changes in cytotechnology practice. Materials and Methods: BOC Practice Analysis Survey data for 2009 and 2015 were examined, comparing survey demographics and performance of laboratory tasks...
July 5, 2018: Laboratory Medicine
https://www.readbyqxmd.com/read/29940246/treatment-and-prevention-of-recurrent-lower-urinary-tract-infections-in-women-a-rapid-review-with-practice-recommendations
#10
REVIEW
Ariana L Smith, Jason Brown, Jean F Wyman, Amanda Berry, Diane K Newman, Ann E Stapleton
PURPOSE: Recurrent lower urinary tract infections in women are a highly prevalent and burdensome condition for which best practice guidelines for treatment and prevention that minimize harm and optimize well-being are greatly needed. To inform development of practice recommendations, a rapid literature review of original research, systematic reviews, meta-analyses and practice guidelines was conducted. MATERIALS AND METHODS: PubMed®, Embase®, Opus, Scopus®, Google Scholar, The Cochrane Library and the U...
June 22, 2018: Journal of Urology
https://www.readbyqxmd.com/read/29928619/systematic-review-and-practice-policy-statements-on-urinary-tract-infection-prevention-in-adults-with-spina-bifida
#11
REVIEW
Michael Tradewell, Joseph J Pariser, Tony Nimeh, Sean P Elliott
Urinary tract infection (UTI) is a source of morbidity and healthcare costs in adults with spina bifida (ASB). UTI prevention strategies are often recommended, but the evidence of various approaches remains unclear. We performed a systematic review to inform a best practice policy statement for UTI prevention in ASB. On behalf of the Neurogenic Bladder Research Group (NBRG.org), we developed an a priori protocol and searched the published English literature for 30 outcomes questions addressing UTI prevention in ASB...
May 2018: Translational Andrology and Urology
https://www.readbyqxmd.com/read/29748329/oral-antibiotic-exposure-and-kidney-stone-disease
#12
Gregory E Tasian, Thomas Jemielita, David S Goldfarb, Lawrence Copelovitch, Jeffrey S Gerber, Qufei Wu, Michelle R Denburg
Background Although intestinal and urinary microbiome perturbations are associated with nephrolithiasis, whether antibiotics are a risk factor for this condition remains unknown. Methods We determined the association between 12 classes of oral antibiotics and nephrolithiasis in a population-based, case-control study nested within 641 general practices providing electronic health record data for >13 million children and adults from 1994 to 2015 in the United Kingdom. We used incidence density sampling to match 25,981 patients with nephrolithiasis to 259,797 controls by age, sex, and practice at date of diagnosis (index date)...
June 2018: Journal of the American Society of Nephrology: JASN
https://www.readbyqxmd.com/read/29670662/effect-of-qingxin-kaiqiao-fang-on-hippocampus-mrna-expression-of-the-inflammation-related-genes-il-1-%C3%AE-gfap-and-a-%C3%AE-in-an-alzheimer-s-disease-rat-model
#13
Dan-Dan Mao, Wen-Yu Yang, Yan Li, Jian-Wei Lin, Shi-Yu Gao, Yi-Ru Wang, Hai-Yan Hu
Objective: To investigate the effects of QKF on expression of amyloid-beta (A β ), interleukin-1 beta (IL-1 β ), and glial fibrillary acidic protein (GFAP) using a rat model of AD. Materials and Methods: Fifty-six male Sprague-Dawley rats were randomly divided into seven groups (eight rats each): control group, sham-operated group, AD model group, groups of AD rats administered with low, medium, and high doses of QKF, and the donepezil group. AD was established by bilateral injection of β -amyloid (A β ) 1-40 into the hippocampus...
2018: Evidence-based Complementary and Alternative Medicine: ECAM
https://www.readbyqxmd.com/read/29669313/role-of-fna-and-special-stains-in-rapid-cytopathological-diagnosis-of-pulmonary-nocardiosis
#14
Ridhi Sood, Ruchita Tyagi, Pavneet Kaur Selhi, Gursheen Kaur, Harpreet Kaur, Akashdeep Singh
BACKGROUND: Nocardia, a gram-positive aerobic bacillus of the Actinomycetales family, is a significant opportunistic pathogen in immunocompromised individuals. Clinical and radiological features of pulmonary nocardiosis are nonspecific and can be misdiagnosed as tuberculosis, pneumocystis, staphylococcal or fungal infections, or as malignancy. Aspiration cytology with special stains is a quick and effective approach for accurate diagnosis. MATERIALS AND METHODS: We present 7 cases of pulmonary nocardiosis, admitted to the pathology department in a tertiary-care hospital in Punjab...
2018: Acta Cytologica
https://www.readbyqxmd.com/read/29665029/grocott-methenamine-silver-and-periodic-acid-schiff-positivity-in-cutaneous-mycobacterium-avium-complex-infection
#15
Robert Schnietz, Morgan L Wilson
No abstract text is available yet for this article.
July 2018: Journal of Cutaneous Pathology
https://www.readbyqxmd.com/read/29664285/core-shell-au-metal-organic-frameworks-for-promoting-raman-detection-sensitivity-of-methenamine
#16
Yanzheng Cai, Yiping Wu, Tong Xuan, Xiaoyu Guo, Ying Wen, Haifeng Yang
Abuse of methenamine in foodstuff is harmful to the health of people. Routine methods recommended by the national standard are indirect assays with complicated pretreatment of samples or less sensitivity. In this work, core-shell Au nanoparticles@inositol hexaphosphate@MIL-101(Fe) nanoparticles, designated as Au@MIL-101, are successfully synthesized by layer-by-layer assembly. Metal-organic framework (MOF; MIL-101)-modified AuNPs could narrow the distance between neighboring Au@MIL-101, which increases the amount of "hot spots" and contributes excellent enhancement of Raman scattering...
May 9, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29655492/isolated-acute-appendicitis-caused-by-aspergillus-in-a-patient-who-underwent-lung-transplantation-a-case-report
#17
H S Kim, H J Yeo, D H Shin, W H Cho, D Kim
Invasive aspergillosis is an important cause of morbidity and mortality in patients who have undergone lung transplantation. Aspergillus infections usually involve the respiratory tract, with vascular invasion and subsequent dissemination. However, acute appendicitis associated with localized aspergillosis is rare, especially among patients who have undergone prophylaxis with voriconazole. We present a case of primary Aspergillus appendicitis diagnosed by histologic examination in a patient who underwent lung transplantation...
May 2018: Transplantation Proceedings
https://www.readbyqxmd.com/read/29608875/blue-green-discoloration-of-urine-and-false-nephrotic-range-proteinuria-at-dipstick-urinalysis
#18
José Antonio Tesser Poloni, Marina Gomes de Moraes Sassi, Tiago Franco de Oliveira, Liane Nanci Rotta, Mark Anthony Perazella
A 32 year-old woman presented to the emergency department with symptoms of urinary tract infection. Over the past 2 days, she reported the use of a medication whose active compounds were methenamine (120 mg) + methylthioninium chloride (20 mg). A collected urine sample had a strong blue-green discoloration. Uroculture was negative and dipstick urinalysis revealed the following results: SG 1.015, pH  7, Albumin 3+, Bilirubin 2+ and Haemoglobin 2+. Urine microscopic analysis revealed 5-6 squamous epithelial cells/high power field (HPF), 5-6 leukocytes/HPF and 7-8 erythrocytes/HPF...
July 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/29556674/recalcitrant-chronic-bladder-pain-and-recurrent-cystitis-but-negative-urinalysis-what-should-we-do
#19
Sheela Swamy, William Barcella, Maria De Iorio, Kiren Gill, Rajvinder Khasriya, Anthony S Kupelian, Jennifer L Rohn, James Malone-Lee
PURPOSE: Lower urinary tract symptoms (LUTS) may be associated with chronic urinary tract infection (UTI) undetected by routine diagnostic tests. Antimicrobial therapy might confer benefit for these patients. MATERIALS AND METHODS: Over 10 years, we treated patients with chronic LUTS. Pyuria was adopted as the principal biomarker of infection. Urinary leucocyte counts were recorded from microscopy of fresh midstream urine (MSU) samples. Antibiotics were prescribed and the prescription adjusted to achieve a measurable clinical response and a reduction in pyuria...
July 2018: International Urogynecology Journal
https://www.readbyqxmd.com/read/29547466/dermalive-facial-filler-granulomas-masquerading-as-neurofibromas
#20
Natalie Wolkow, Frederick A Jakobiec, Michael K Yoon
A 56-year-old woman presented with periocular nodules that were clinically suspected to be neurofibromas. Histopathologic examination of excised nodules revealed a pronounced granulomatous reaction to a foreign material that was composed of glossy polygonal palely eosinophilic fragments. These fragments were outlined in red with Masson trichrome, stained gray with the elastic stain, and were uniformly red with Gomori methenamine silver staining. The histopathologic appearance was consistent with a granulomatous reaction to Dermalive facial filler...
May 2018: Ophthalmic Plastic and Reconstructive Surgery
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