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Temperature buffer

John P Rippe, Justin H Baumann, Daphne N De Leener, Hannah E Aichelman, Eric B Friedlander, Sarah W Davies, Karl D Castillo
Through the continuous growth of their carbonate skeletons, corals record information about past environmental conditions and their effect on colony fitness. Here, we characterize century-scale growth records of inner and outer reef corals across ~200 km of the Florida Keys Reef Tract (FKRT) using skeletal cores extracted from two ubiquitous reef-building species, Siderastrea siderea and Pseudodiploria strigosa. We find that corals across the FKRT have sustained extension and calcification rates over the past century but have experienced a long-term reduction in skeletal density, regardless of reef zone...
August 13, 2018: Global Change Biology
Natalie E Stenzoski, Bowu Luan, Alex S Holehouse, Daniel P Raleigh
The temperature dependence of the overall dimensions of the denatured state ensemble (DSE) of proteins remains unclear. Some studies indicate compaction of the DSE at high temperatures, whereas others argue that dimensions do not decrease. The degree of compaction or expansion in the cold-denatured state has been less studied. To investigate the temperature dependence of unfolded state dimensions, small angle x-ray scattering measurements were performed in native buffer in the absence of denaturant for a designed point mutant of the C-terminal domain of L9, a small cooperatively folded α-β protein, at 14 different temperatures over the range of 5-60°C...
July 23, 2018: Biophysical Journal
Lucy Haque, Sutanwi Bhuiya, Indrajit Giri, Susmita Chowdhury, Suman Das
Polymorphism of DNA plays a very important part of research relating to the drug-DNA interactions. Here main focus of our investigation is to monitor the interaction of the benzophenanthridine plant alkaloid, nitidine (NIT) with two different forms of DNA i.e. B-DNA and protonated form of DNA maintaining proper temperatures and buffer conditions. Binding interaction of NIT was ascertained from the UV-Visible spectroscopic and spectrofluorimetric titration experiments. Binding constants of the interactions of NIT with different polymorphic forms were calculated from UV-absorption study...
August 8, 2018: International Journal of Biological Macromolecules
Biji T Kurien, R Hal Scofield
It is customary to dry gels (SDS-PAGE, native gels, or two-dimensional gels) after staining for record keeping purposes. This is typically carried out with gel dryers or by drying between two cellophane sheets held together by acrylic frames. Here, we report a simple method to store a variety of stained gels without any storage buffer within flexible nonsealed polyethylene bags. Gels can be stored for several months at room temperature without significant shrinking or protein diffusion. The gel stays hydrated owing to the de facto sealing achieved by the polyethylene sheets adhering air-tightly to the gel on either side...
2018: Methods in Molecular Biology
Fazhan Shi, Fei Kong, Pengju Zhao, Xiaojun Zhang, Ming Chen, Sanyou Chen, Qi Zhang, Mengqi Wang, Xiangyu Ye, Zhecheng Wang, Zhuoyang Qin, Xing Rong, Jihu Su, Pengfei Wang, Peter Z Qin, Jiangfeng Du
Magnetic resonance spectroscopy of single biomolecules under near-physiological conditions could substantially advance understanding of their biological function, but this approach remains very challenging. Here we used nitrogen-vacancy centers in diamonds to detect electron spin resonance spectra of individual, tethered DNA duplexes labeled with a nitroxide spin label in aqueous buffer solutions at ambient temperatures. This work paves the way for magnetic resonance studies on single biomolecules and their intermolecular interactions in native-like environments...
August 6, 2018: Nature Methods
Gangtao Wu, Yaling Wang, Kexin Zhang, Yuying Huang, Lijun Sun, Chaojin Wu, Jianrong Li
OBJECTIVE: Preparing immunomagnetic beads for enrichment and purification of mask type A trichothecenes. METHODS: Through immunizing New Zealand white rabbits with family A toxin artificial antigen 3-Ac-NEOS-HS-BSA, the polyclonal antibodies of type A trichothecenes( type A family) were isolated, as well as its antibody titre were determinated and purified. The coupling condition of high titer antibody and immunomagnetic beads were optimized and applied. RESULTS: After 5 times of immunization with type A trichothecene family toxin, the rabbit serum inhibited the highest antibody titer( 1 : 64 000)...
July 2018: Wei Sheng Yan Jiu, Journal of Hygiene Research
P Morlière, A M S Silva, R S G R Seixas, F Boscá, J-C Mazière, J Ferreira, R Santus, P Filipe
The phototoxicity of voriconazole (VN) prescribed in the treatment of severe fungal infections is frequently reported. Its major metabolite, a N-oxide derivative (VNO), was suspected to be the photosensitizer because it shows a maximum absorbance at ~310 nm in aqueous solutions. It was reported that the VNO photoproduct (VNOP) was phototoxic to human keratinocytes. Steady state and laser flash photolyses were performed to shed light on the phototoxic properties of VNO and VNOP. The quantum yield of the VNOP production by UVB-UVA light in buffered or alcoholic solutions is 0...
July 31, 2018: Journal of Photochemistry and Photobiology. B, Biology
Mahboubeh Rezazadeh, Niloofar Jafari, Vajihe Akbari, Marjan Amirian, Majid Tabbakhian, Mohsen Minaiyan, Mahboubeh Rostami
Oral mucositis (OM) represents a therapeutic challenge frequently encountered in cancer patients undergoing chemotherapy or radiotherapy. Erythropoietin (EPO) has anti-inflammatory, antioxidant, and wound-healing properties and therefore has important roles in the prevention and treatment of OM. In the current study, we developed a thermally sensitive mucoadhesive gel based on trimethyl chitosan (TMC) containing EPO for the treatment of OM. TMCs with various degrees of substitution (DS) were synthesized and mixed with β-glycerophosphate (GP) and characterized for gelation properties by means of rheological analysis...
August 3, 2018: Drug Delivery and Translational Research
Rita Gelli, Martino Scudero, Lucia Gigli, Mirko Severi, Massimo Bonini, Francesca Ridi, Piero Baglioni
HYPOTHESIS: Amorphous Magnesium-Calcium Phosphate (AMCP) particles in the distal small intestine have been shown to have a fundamental role in mammals' immune-surveillance mechanisms. Their formation in the gut lumen and their stability against crystallization are expected to depend upon physiological conditions such as pH and [Mg2+ ]. Knowing the influence of these parameters on AMCP stability would allow to predict the presence and the activity of the particles in physiological or pathological conditions...
July 24, 2018: Journal of Colloid and Interface Science
Miguel A Redondo, Johanna Boberg, Jan Stenlid, Jonàs Oliva
Diversity of microbial organisms is linked to global climatic gradients. The genus Phytophthora includes both aquatic and terrestrial plant pathogenic species that display a large variation of functional traits. The extent to which the physical environment (water or soil) modulates the interaction of microorganisms with climate is unknown. Here, we explored the main environmental drivers of diversity and functional trait composition of Phytophthora communities. Communities were obtained by a novel metabarcoding setup based on PacBio sequencing of river filtrates in 96 river sites along a geographical gradient...
August 2, 2018: ISME Journal
Ricardo Bezerra Costa, Patricia Targon Campana, Felipe Santiago Chambergo, Thiago Henrique Napoleão, Patrícia Maria Guedes Paiva, Hugo Juarez Vieira Pereira, Maria Luiza Vilela Oliva, Francis Soares Gomes
Genipa americana L., commonly known as genipap, is a plant with economical and medicinal importance, and a promising source of bioactive compounds. Lectins are carbohydrate-binding proteins with several biotechnological applications. This study reports the isolation and characterization of a G. americana bark lectin (GaBL). A single chromatographic procedure on Sephacryl S-100 resulted in isolation of GaBL, a protein with native molecular weight of over 200 kDa and pI 4.02, whose hemagglutinating activity was inhibited by lactose and fetuin, not affected by ions (Ca2+ and Mg2+ ), and stable upon heating (303-393 K) as well as over the pH range 5-10...
July 29, 2018: International Journal of Biological Macromolecules
Benjamin J Brownlee, Meisam Bahari, John N Harb, Jonathan C Claussen, Brian D Iverson
Free-standing, vertically aligned carbon nanotubes (VACNTs) were patterned into 16 µm diameter microchannel arrays for flow-through electrochemical glucose sensing. Non-enzymatic sensing of glucose was achieved by the chemical reaction of glucose with methyl viologen (MV) at an elevated temperature and pH (0.1 M NaOH), followed by the electrochemical reaction of reduced-MV with the VACNT surface. The MV sensor required no functionalization (including no metal) and was able to produce on average 3.4 electrons per glucose molecule...
August 1, 2018: ACS Applied Materials & Interfaces
Andre Jung, Oliver Weichold
Highly alkaline hydrogels that allow the restoration of alkaline buffer in cementitious materials can be obtained from diallyldimethylammonium hydroxide. The latter must be prepared in dilute solutions and polymerised at ambient temperatures in order to avoid decomposition. Using methacrylamide as a neutral co-monomer capable of forming hydrogen bonds, the rheological properties of the gels can be adjusted over a wide range; e.g. the viscosity increases a thousandfold from 0.35 Pa s to >350 Pa s by using 10 mol% methacrylamide...
August 1, 2018: Soft Matter
Naser Bazina, Jibin He
During the deep fat food frying process, the frying media, oil, continuously degenerates when exposed to high temperature, oxygen and moisture. This leads to physical and chemical changes including the formation of hydrolysis products such as free fatty acids (FFAs) which are associated with undesirable darkening in colour, off-flavouring and a lowering of the smoke point. This study was aiming to develop a method capable of identifying and quantifying individual free fatty acids within oil using a small sample size (100 mg of oil)...
August 2018: Journal of Food Science and Technology
Lydia Kisley, Kali A Serrano, Caitlin M Davis, Drishti Guin, Elizabeth Murphy, Martin Gruebele, Deborah E Leckband
The widespread interest in neutral, water-soluble polymers such as poly(ethyleneglycol) (PEG) and poly(zwitterions) such as poly(sulfobetaine) (pSB) for biomedical applications is due to their widely assumed low protein binding. Here we demonstrate that pSB chains in solution can interact with proteins directly. Moreover, pSB can reduce the thermal stability and increase the protein folding cooperativity relative to proteins in buffer or in PEG solutions. Polymer-dependent changes in the tryptophan fluorescence spectra of three structurally distinct proteins reveal that soluble, 100 kDa pSB interacts directly with all three proteins and changes both the local polarity near tryptophan residues and the protein conformation...
July 31, 2018: Biomacromolecules
Madiha Moid, Sidra Afzal, Najia Rahim, Tariq Ali, Wajiha Iffat, Lubna Bashir, Shazia Naz
A precise, sensitive and quick High Performance Liquid Chromatographic (HPLC) method for the determination of rosuvastatin calcium in bulk and tablet dosage forms has been validated. The chromatographic scheme involved: Sil-20A auto sampler, LC-20A pump, SPD-20A UV/visible detector with separation attained by C18 column at 40ºC temperature through a mobile phase of acetonitrile and buffer (50:50) at a flow rate of 1.0ml/min. The method is precise (%RSD for intra-day and inter-day extended between 1.06-1.54% and 0...
July 2018: Pakistan Journal of Pharmaceutical Sciences
Chloé R Nater, Koen J van Benthem, Cindy I Canale, Carsten Schradin, Arpat Ozgul
1.Population dynamics are the result of an interplay between extrinsic and intrinsic environmental drivers. Predicting the effects of environmental change on wildlife populations therefore requires a thorough understanding of the mechanisms through which different environmental drivers interact to generate changes in population size and structure. 2.In this study, we disentangled the roles of temperature, food availability, and population density in shaping short- and long-term population dynamics of the African striped mouse, a small rodent inhabiting a semi-desert with high intra- and inter-annual variation in environmental conditions...
July 30, 2018: Journal of Animal Ecology
Akinrinade George Ayankojo, Jekaterina Reut, Andres Öpik, Andreas Furchner, Vitali Syritski
The potential adverse effects of the environmental presence of antibiotics on the ecosystem demands the development of new methods suitable for accurate detection of these micropollutants in various aquatic media. An analytical method exploiting the synergistic effect of a label-free sensing platform combined with a molecularly imprinted polymer (MIP) as robust recognition element could represent an efficient tool for the real-time monitoring of antibiotics. In this work, a hybrid organic-inorganic MIP film (AMO-MIP) selective towards amoxicillin (AMO) was synthesized and integrated with a surface plasmon resonance (SPR) sensor...
July 19, 2018: Biosensors & Bioelectronics
Fernando Ajejas, Adrian Gudin, Ruben Guerrero, Alberto Anadon, Jose Manuel Diez, Leticia de Melo Costa, Pablo Olleros, Miguel Angel Niño, Stéfania Pizzini, Jan Vogel, Manuel Valvidares, Pierluigi Gargiani, Mariona Cabero, María Varela, Rodolfo Miranda, Julio Camarero, Paolo Perna
A major challenge for future spintronics is to develop suitable spin transport channels with long spin lifetime and propagation length. Graphene can meet these requirements, even at room temperature. On the other side, taking advantage of the fast motion of chiral textures, i.e., Néel-type domain walls and magnetic skyrmions, can satisfy the demands for high-density data storage, low power consumption and high processing speed. We have engineered epitaxial structures where an epitaxial ferromagnetic Co layer is sandwiched between an epitaxial Pt(111) buffer grown in turn onto MgO(111) substrates and a graphene layer...
July 27, 2018: Nano Letters
M Manjushree, Hosakere D Revanasiddappa
The binding interaction between bovine serum albumin (BSA) and sodium salt of risedronic acid (RSN) was studied by using the FT-IR (Fourier transform infrared), UV-Vis (ultraviolet-visible), fluorescence (emission and synchronous), CD (circular dichroism) spectrometric, and computational (molecular docking) techniques at 289, 297, and 305 K temperatures with physiological buffer of pH 7.40. The conformational and secondary structural changes observed for BSA from CD spectra and by curve fitting procedure were applied to Fourier self-deconvolution in FT-IR spectra...
2018: Bioinorganic Chemistry and Applications
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