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Temperature buffer

Aleksandra Radtke, Marlena Grodzicka, Michalina Ehlert, Tadeusz M Muzioł, Marek Szkodo, Michał Bartmański, Piotr Piszczek
Dispersed silver nanoparticles (AgNPs) on the surface of titanium alloy (Ti6Al4V) and titanium alloy modified by titania nanotube layer (Ti6Al4V/TNT) substrates were produced by the chemical vapor deposition method (CVD) using a novel precursor of the formula [Ag₅(O₂CC₂F₅)₅(H₂O)₃]. The structure and volatile properties of this compound were determined using single crystal X-ray diffractometry, variable temperature IR spectrophotometry (VT IR), and electron inducted mass spectrometry (EI MS). The morphology and the structure of the produced Ti6Al4V/AgNPs and Ti6Al4V/TNT/AgNPs composites were characterized by scanning electron microscopy (SEM) and atomic force microscopy (AFM)...
December 9, 2018: International Journal of Molecular Sciences
Chandana Kolluru, Yasmine Gomaa, Mark R Prausnitz
The aim of this study was to develop a dissolving microneedle (MN) patch for administration of inactivated polio vaccine (IPV) with improved thermal stability when compared with conventional liquid IPV. Excipient screening showed that a combination of maltodextrin and D-sorbitol in histidine buffer best preserved IPV activity during MN patch fabrication and storage. As determined by D-antigen ELISA, all three IPV serotypes maintained > 70% activity after 2 months and > 50% activity after 1-year storage at 5 °C or 25 °C with desiccant...
December 12, 2018: Drug Delivery and Translational Research
Natalia Maciejewska, Rafał Walkusz, Marcin Olszewski, Aneta Szymańska
Nucleases are an important group of hydrolases that degrade nucleic acids, with broad spectrum of applications in science and industry. In this paper, we report the identification and characterization of the nuclease from extremely psychrophilic bacterium Psychromonas ingrahamii that grows exponentially at 5 °C, but may also grow at even lower temperatures (down to - 12 °C). The putative endonuclease I gene, identified in the genome of P. ingrahamii, was cloned and expressed in Pichia pastoris. The recombinant protein was purified and its nucleolytic features were studied...
December 11, 2018: Molecular Biotechnology
Norbert Rácz, Júlia Nagy, Wen Jiang, Tibor Veress
The goal of this work was to investigate and compare the selectivity of three different hydrophilic interaction liquid chromatography (HILIC) charge modulated amide columns, iHILIC®-Fusion, iHILIC®-Fusion(+) and iHILIC®-Fusion(P), for analysis of compounds in hallucinogen mushrooms. An extract of a truffle-like fungus containing psilocin, psilocybin and baeocystin was chosen as test material. Three different modeling methods were applied to describe the retention times of constituents in isocratic separation mode as a function of mobile phase composition, pH and temperature...
December 7, 2018: Journal of Chromatographic Science
Ryan J Leonard, Katie K Y Wat, Clare McArthur, Dieter F Hochuli
Changes in the mean and variance of phenotypic traits like wing and head morphology are frequently used as indicators of environmental stress experienced during development and may serve as a convenient index of urbanization exposure. To test this claim, we collected adult western honey bee ( Apis mellifera Linnaeus 1758, Hymenoptera, Apidae) workers from colonies located across an urbanization gradient, and quantified associations between the symmetries of both wing size and wing shape, and several landscape traits associated with urbanization...
2018: PeerJ
R B Verheijen, B Thijssen, F Atrafi, J H M Schellens, H Rosing, N de Vries, J H Beijnen, R H J Mathijssen, N Steeghs, A D R Huitema
Everolimus is a mammalian target of rapamycin inhibitor approved for the treatment of various tumor types. Less invasive measurement of everolimus concentrations could facilitate pharmacokinetic studies and personalized dosing based on whole blood concentrations, known as therapeutic drug monitoring. Volumetric Absorptive Microsampling (VAMS) has been introduced as a patient friendly, less invasive sampling technique to obtain an accurate volume of whole blood regardless of hematocrit value. We describe the bioanalytical validation and clinical application of a liquid chromatography tandem mass spectrometry (LC-MS/MS) method to quantify everolimus using VAMS...
November 30, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Lin Ye, Xiang Liu, Guang-Hui Shen, Shan-Shan Li, Qing-Ying Luo, He-Jun Wu, An-Jun Chen, Xing-Yan Liu, Mei-Liang Li, Biao Pu, Wen Qin, Zhi-Qing Zhang
In this paper, the catalytic performance of non-purified esterase from wheat bran immobilized on glass fibre membrane carrier is established, the immobilization conditions observed were enzyme 1 mL, phosphate buffer 3 mL (pH 7.0), immobilization time 1 h, immobilization temperature 29 °C. After carrier functionalization some characteristics of immobilized enzyme were studied, the results showed that immobilized enzyme presenting improved characteristic than that of free enzyme. The optimum pH for free and immobilized enzymes were found to be 8 and 7, respectively...
December 5, 2018: International Journal of Biological Macromolecules
Liang Zhu, Lin Wang, Chun-Sen Ma
Climate warming is characterized by increase in extreme heat events (EHEs). EHEs and mild temperature periods alternate with each other and form complex climate scenarios. Among these scenarios, low-frequency and short-duration extreme heat events during long mild periods (sporadic short EHEs) and low-frequency and short-duration mild periods during long extreme heat events (sporadic short mild periods) commonly occur in nature. The biological effects of these two types of temperature events have not been thoroughly elucidated to date...
December 5, 2018: Journal of Insect Physiology
L Kleinjans, D H Veening-Griffioen, T Wehkamp, J van Bergenhenegouwen, J Knol, J Garssen, L M J Knippels, C Belzer, P V Jeurink
Non-breastfed infants at-risk of allergy are recommended to use a hydrolysed formula before the age of 6 months. The addition of prebiotics to this formula may reduce the allergy development in these infants, but clinical evidence is still inconclusive. This study evaluates (1) whether the exposure duration to different prebiotics alongside a partially hydrolysed whey protein (pHP) influences its' effectiveness to prevent allergy development and (2) whether the gut microbiota plays a role in this process. Mice orally sensitised with whey and/or cholera toxin were orally treated for six days before sensitization with phosphate buffered saline, whey or pHP to potentially induce tolerance...
December 10, 2018: Beneficial Microbes
Nur Fattima' Al-Zahara' Tuan Mohamood, Norhazlin Zainuddin, Mansor Ahmad Ayob, Sheau Wei Tan
In this study, sago starch was modified in order to enhance its physicochemical properties. Carboxymethylation was used to introduce a carboxymethyl group into a starch compound. The carboxymethyl sago starch (CMSS) was used to prepare smart hydrogel by adding acetic acid into the CMSS powder as the crosslinking agent. The degree of substitution of the CMSS obtained was 0.6410. The optimization was based on the gel content and degree of swelling of the hydrogel. In this research, four parameters were studied in order to optimize the formation of CMSS-acid hydrogel...
December 6, 2018: Chemistry Central Journal
Fabio Formenti, Giulia La Marca, Francesca Perandin, Barbara Pajola, Miryam Romano, Beatrice Santucci, Ronaldo Silva, Giovanni Giorli, Zeno Bisoffi, Dora Buonfrate
Strongyloides stercoralis is a soil-transmitted helminth with a wide distribution in tropical and subtropical areas. The diagnosis of S. stercoralisinfection can be challenging, due to the low sensitivity of microscopic examination of stool samples and coproculture. In the last decade, different in-house molecular biology techniques for S. stercoralis have been implemented. They demonstrated good accuracy, although sensitivity does not seem sufficiently high yet. Recently, a novel PCR technique has been evaluated for the detection of S...
December 3, 2018: Acta Tropica
Jun Shi, Yanting Wu, Siyi Guo, Huidi Zhang, Guitian Chen, Xiaoqi Xu
The aim of this study was to investigate the efficacy of anti-VEGF antibody-modified Paeonol liposome gels (PAE-BEV-lip gels) in the prevention and treatment of Hypertrophic Scars (HSs). Systematic optimization of the encapsulation process of anti-VEGF antibody-modified Paeonol liposomes (PAE-BEV-lips) was performed using Box-Behnken design with the optimized parameters as follows: SPC concentration of 7.36 mg·mL-1 ; SPC-Chol-PAE:pNP-PEG3000 -DOPE:BVE-PEG3000 -DOPE ratio of 14:5:4:0.28:0.05, w/w; the hydration temperature of 41 °C; stripping using pH 7...
December 4, 2018: Drug Development and Industrial Pharmacy
Ombeline Danton, Lara Alexander, Cindy Hunlun, Dalene de Beer, Matthias Hamburger, Elizabeth Joubert
A naringenin derivative, isolated from Cyclopia genistoides, a bitter tasting herbal tea, especially when in green (unoxidized) form, was identified as (2 S)-5-[α-l-rhamnopyranosyl-(1→2)-β-d-glucopyranosyloxy]naringenin (1). The compound partially epimerizes to (2 R)-5-[α-l-rhamnopyranosyl-(1→2)-β-d-glucopyranosyloxy]naringenin (2) when heated at different temperatures (80, 90, 100, 110, and 120 °C) for a prolonged period in a phosphate buffer at pH 5. The fractional conversion model predicted the decrease in the concentration of compound 1 the best...
December 4, 2018: Journal of Natural Products
R de Jonge
In previous work, extreme survival of various bacterial species during cooking was reported when attached to chicken meat. In this paper the effects of an extremely high challenge temperature on survival of Salmonella Typhimurium and Campylobacter jejuni, attached to chicken breast fillets or pork to test for matrix effects are reported. Survival was predicted, using standard D- and z-values from the literature, and compared to experimentally obtained data. Attached to meat, both S. Typhimurium and C. jejuni survived longer than predicted, longer when attached to chicken meat than when attached to pork...
November 20, 2018: International Journal of Food Microbiology
M Ines Burgos, Aylen Ochoa, María A Perillo
The effect on protein conformation and thermal stability was studied for β-Galactosidase (β-Gal) encapsulated in the nanopores of a silicate matrix (Eβ-Gal ). Circular dichroism spectra showed that, compared with the enzyme in buffer (Sβ-Gal ), Eβ-Gal exhibited a higher content of α-helix structure. Heating Eβ-Gal up to 75 °C caused a decrease in the content of β-sheet structure and additional augments on Eβ-Gal components attributed to helical content, instead of the generalized loss of the ellipticity signal observed with Sβ-Gal ...
November 26, 2018: Biochemical and Biophysical Research Communications
W C Kayser, G E Carstens, I L Parsons, T H Welsh, K E Washburn, S D Lawhon, W E Pinchak, J T Richeson, E Chevaux, A L Skidmore
Objectives of this experiment were to examine the effects of live yeast (LY) supplementation on immunological, physiological and behavioral responses in steers experimentally challenged with Mannheimia haemolytica (MH). Thirty-six crossbred Angus steers (BW = 352 ± 23 kg) seronegative for MH were allocated within a 2 x 2 factorial arrangement: Factor 1= roughage-based diet with LY (Saccharomyces cerevisiae boulardii CNCM I-1079, 25 g·per steer daily) or negative control (CON). Factor 2 = bronchoselective endoscopic inoculation with MH or phosphate buffer solution (PBS)...
November 28, 2018: Journal of Animal Science
Sang Phil Shin, Han Chang Sohn, Chang Nam Jin, Bong Jo Kang, Jehee Lee
Enteromyxum leei has been reported to cause emaciation disease in various fish species. To determine the effect of parasite intensity on cultured olive flounder Paralichthys olivaceus, we investigated the relationship between the relative condition factor (rCF = CF/standard CF × 100) and parasite load with quantitative polymerase chain reaction (qPCR) and the challenge test. A total of 57 cultured olive flounders were obtained from 11 fish farms and divided into five groups based on their rCF. We investigated the parasite intensity in the posterior intestine of the fish...
November 29, 2018: Journal of Fish Diseases
Fernanda G Amorim, Danilo L Menaldo, Sante E I Carone, Thiago A Silva, Marco A Sartim, Edwin De Pauw, Loic Quinton, Suely V Sampaio
Snake venom serine proteases (SVSPs) are enzymes that are capable of interfering in various parts of the blood coagulation cascade, which makes them interesting candidates for the development of new therapeutic drugs. Herein, we isolated and characterized Moojase, a potent coagulant enzyme from Bothrops moojeni snake venom. The toxin was isolated from the crude venom using a two-step chromatographic procedure. Moojase is a glycoprotein with N-linked glycans, molecular mass of 30.3 kDa and acidic character (pI 5...
November 28, 2018: Toxins
Nikki A Thiele, Samantha N MacMillan, Justin J Wilson
Insoluble BaSO4 scale is a costly and time-consuming problem in the petroleum industry. Clearance of BaSO4 -impeded pipelines requires chelating agents that can efficiently bind Ba2+ , the largest nonradioactive +2 metal ion. Due to the poor affinity of currently available chelating agents for Ba2+ , however, the dissolution of BaSO4 remains inefficient, requiring very basic solutions of ligands. In this study, we investigated three diaza-18-crown-6 macrocycles bearing different pendent arms for the chelation of Ba2+ and assessed their potential for dissolving BaSO4 scale...
November 28, 2018: Journal of the American Chemical Society
Jofre Carnicer, Constantí Stefanescu, Maria Vives-Ingla, Carlos López, Sofia Cortizas, Christopher Wheat, Roger Vila, Joan Llusià, Josep Peñuelas
1.Widespread population declines have been reported for diverse Mediterranean butterflies over the last three decades, and have been significantly associated to increased global change impacts. The specific landscape and climatic drivers of these declines remain uncertain for most declining species. 2.Here we analyse whether plastic phenotypic traits of a model butterfly species (Pieris napi) perform as reliable biomarkers of vulnerability to extreme temperature impacts in natural populations, showing contrasting trends in thermally exposed and thermally buffered populations...
November 27, 2018: Journal of Animal Ecology
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