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Temperature buffer

Mengsi Cao, Yanru Feng, Yan Zhang, Weijun Kang, Kaoqi Lian, Lianfeng Ai
Vancomycin is one of the most commonly used glycopeptide antiobiotics, and as such is an important emerging environmental contaminant. Pharmaceuticals and personal care products (PPCPs), such as antibiotics, are problematic since wastewater treatment processes are not completely effective at removing these chemical compounds. Since wastewater treatment processes are not completely effective, vancomycin occurs in surface water. Vancomycin and its metabolites in vivo and degradation products in aquatic environment may lead to undesirable ecological effects that threaten the environment or cause undesirable reactions that affect human health...
October 19, 2018: Scientific Reports
Chao Zhu, Xiaoqian Wang, Linsen Li, Chenxu Hao, Youhao Hu, Aysha Sarfraz Rizvi, Feng Qu
CE application in aptamer selection (CE-SELEX) shows more advantages than other selection methods. In this study, an online reaction based single-step CE (ssCE) mode was employed for fast obtaining protein-ssDNA complex. Using human thrombin (H-Thr) and its aptamer Apt29 as models, we accomplished the procedures of mixing, reaction, separation, detection and complex collection in single step online process, which took about 10 min to obtain the H-Thr/Apt29 complex. Important factors, affecting the aptamer and H-Thr interaction (buffer, ratio of aptamer and H-Thr amount), and complex separation and collection (voltage and temperature) were discussed...
October 16, 2018: Biochemical and Biophysical Research Communications
Marco Bragato, Simona Achilli, Fausto Cargnoni, Davide Ceresoli, Rocco Martinazzo, Raffaella Soave, Mario Italo Trioni
We report the electronic, magnetic and transport properties of a prototypical antiferromagnetic (AFM) spintronic device. We chose Cr as the active layer because it is the only room-temperature AFM elemental metal. We sandwiched Cr between two non-magnetic metals (Pt or Au) with large spin-orbit coupling. We also inserted a buffer layer of insulating MgO to mimic the structure and finite resistivity of a real device. We found that, while spin-orbit has a negligible effect on the current flowing through the device, the MgO layer plays a crucial role...
October 18, 2018: Materials
Yawen Li, Donald K Hamlin, Ming-Kuan Chyan, Roger Wong, Eric F Dorman, Robert C Emery, Douglas R Woodle, Ronald L Manger, Margaret Nartea, Aimee L Kenoyer, Johnnie J Orozco, Damian J Green, Oliver W Press, Rainer Storb, Brenda M Sandmaier, D Scott Wilbur
The objective of this study was to translate reaction conditions and quality control methods used for production of an astatine-211(211At)-labeled anti-CD45 monoclonal antibody (MAb) conjugate, 211At-BC8-B10, from the laboratory setting to cGMP production. Five separate materials were produced in the preparation of 211At-BC8-B10: (1) p-isothiocyanato-phenethyl-closo-decaborate(2-) (B10-NCS), (2) anti-CD45 MAb, BC8, (3) BC8-B10 MAb conjugate, (4) [211At]NaAt, and (5) 211At-BC8-B10. The 211At-labeling reagent, B10-NCS, was synthesized as previously reported...
2018: PloS One
Aljosa Trmcic, Huihui Chen, Monika Trząskowska, Sandeep Tamber, Siyun Wang
Salmonella enterica is one of the pathogens that is frequently identified as the cause of fresh produce-related outbreaks. Biofilm formation is a factor that can contribute to pathogen survival on produce surface. The goal of our current research was to investigate the survival of five S. enterica strains representing different serotypes (i.e., Typhimurium, Enteritidis, Daytona, Poona, and Newport) on whole mini cucumbers stored at refrigeration (4°C) and room temperature (22°C). We also determined the strains survival on glass slides and in phosphate-buffered saline at 4 and 22°C, as well as the ability to form biofilms on a solid-liquid interphase...
October 16, 2018: Journal of Food Protection
Pan Liu, Chun Ying Ren, Zong Ming Wang, Bai Zhang, Lin Chen
Scientific and rapid assessment of the ecological environment quality based on remote sensing can provide basis for regional protection, management and planning. In this study, using Landsat TM/OLI/TIRS in 1990, 2000 and 2015 as data sources, four indices including the wetness component of the tasseled cap transformation (WET), normalized difference vegetation index (NDVI), normalized difference built-up and soil index (NDSI), and land surface temperature (LST) were derived, with the weights being determined by principal components analysis (PCA)...
October 2018: Ying Yong Sheng Tai Xue Bao, the Journal of Applied Ecology
Rona Shaharabani, Maor Ram-On, Yeshayahu Talmon, Roy Beck
Multiple sclerosis (MS) is an autoimmune disease, leading to the destruction of the myelin sheaths, the protective layers surrounding the axons. The etiology of the disease is unknown, although there are several postulated environmental factors that may contribute to it. Recently, myelin damage was correlated to structural phase transition from a healthy stack of lamellas to a diseased inverted hexagonal phase as a result of the altered lipid stoichiometry and low myelin basic protein (MBP) content. In this work, we show that environmental conditions, such as buffer salinity and temperature, induce the same pathological phase transition as in the case of the lipid composition in the absence of MBP...
October 15, 2018: Proceedings of the National Academy of Sciences of the United States of America
E Morrissey-Mccaffrey, S Shephard, F L Kelly, M Kelly-Quinn
This study finds that non-native species and warming temperatures have significant negative effects on Arctic char Salvelinus alpinus abundance in Irish lakes. Eutrophication was not important at the range of total phosphorus tested (0.005-0.023 mg l-1 ). Model results predict that S. alpinus occur across the temperature range sampled (8.2-19.7°C) when non-natives are absent, but S. alpinus catch is predicted to be close to zero irrespective of temperature when non-native catch is high. This result indicates that to persist S...
October 12, 2018: Journal of Fish Biology
Harriet Swearman, George Koustas, Emily Knight, Georgios Liperis, Christopher Grupen, Cecilia Sjoblom
RESEARCH QUESTION: Temperature fluctuation negatively impacts the assembly and function of the meiotic spindle, but does pH have a similar effect? DESIGN: Polarized light microscopy was used to study the spindle in living mouse oocytes under different pH conditions. Female mice (n = 53) were superovulated, and oocytes collected, denuded and allocated to treatment groups. All experiments were performed at 37°C, and standard bicarbonate-buffered medium was used either pre-equilibrated in 6% CO2 or unequilibrated (in ambient CO2 )...
September 2018: Reproductive Biomedicine Online
Md Ariful Alam, Sheikh Mustafizur Rahman, Yoji Yamamoto, Ricardo Shohei Hattori, Toru Suzuki, Manabu Watanabe, Carlos Augusto Strüssmann
Microinjection has proven useful for introduction of low-permeability cryoprotective agents (CPAs) into fish eggs or embryos for cryopreservation. In this work, we examined the suitable conditions for single or combined microinjection into the perivitelline space (PS) and the yolk mass (YM) of embryos of the Japanese whiting, an alternative marine fish model for embryo cryopreservation studies. The parameters examined were injection volume, CPA type and concentration, vehicle (diluent), and suitable developmental stage...
October 9, 2018: Cryobiology
Quan Han, Yanyan Huo, Longhu Yang, Xiaohui Yang, Yaping He, Jiangyan Wu
A simple and sensitive cloud point extraction method for the preconcentration of ultra-trace amounts of nickel as a prior step to its determination by graphite furnace atomic absorption spectrometry was proposed. It is based on the reaction of nickel with 2-(5-bromo-2-pyridylazo)-5-dimethylaminoaniline (5-Br-PADMA) in HAc⁻NaAc buffer media and mixed micelle-mediated extraction of the complex using the anionic surfactant sodium dodecyl sulfate sodium (SDS) and non-ionic surfactant (1,1,3,3-Tetramethylbutyl)phenyl-polyethylene (Triton X-114)...
October 10, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Cong Wang, Zhen Li, Zhongli Pan, Daoliang Li
The determination of nitrate nitrogen (NO₃-N) in recirculating aquaculture systems is of great significance for the health assessment of the living environment of aquatic animals. Unfortunately, the commonly used spectrophotometric methods often yield unstable results, especially when the ambient temperature varies greatly in the field measurement. Here, we have developed a novel handheld absorbance measurement sensor based on the thymol-NO₃-N chromogenic rearrangement reaction. In terms of hardware, the sensor adopts a dual channel/dual wavelength colorimeter structure that features a modulated light source transmitter and a synchronous detector receiver...
October 10, 2018: Sensors
Pratibha Kumari, Lukas Frey, Alexander Sobol, Nils-Alexander Lakomek, Roland Riek
15 N R2 relaxation measurements are key for the elucidation of the dynamics of both folded and intrinsically disordered proteins (IDPs). Here we show, on the example of the intrinsically disordered protein α-synuclein and the folded domain PDZ2, that at physiological pH and near physiological temperatures amide-water exchange can severely skew Hahn-echo based 15 N R2 relaxation measurements as well as low frequency data points in CPMG relaxation dispersion experiments. The nature thereof is the solvent exchange with deuterium in the sample buffer, which modulates the 15 N chemical shift tensor via the deuterium isotope effect, adding to the apparent relaxation decay which leads to systematic errors in the relaxation data...
October 10, 2018: Journal of Biomolecular NMR
Hirofumi Ochiai, Kenta Yoshida, Hiroshi Shibutani, Akio Kanatani, Yuji Nishiuchi
Reversibly glycosylated conjugates were developed by adding complex-type N-linked oligosaccharides to peptides through self-cleavable linkers with the aim of increasing the solubility and stability of the peptides in plasma. The amino or carboxyl group of the peptide was connected to a glycosylated Ascendis or ester/thioester-type linker, respectively. Use of the linkers enabled extended release of the peptides depending on the pH and temperature of the buffer according to a first order reaction, and their cleavage rate was also affected by the structure of the peptide-linker coupling...
October 10, 2018: Chemical & Pharmaceutical Bulletin
Jie Li, XuZhao Zhai, Chengchao Ding, Yali Liu, Qingli Dong, Dongpo Xu, Xiang Wang, Jingxuan Qiu, Qi Zhang, Jing Pan, Qing Liu
Hybridoma screening is a key step for the successful generation of high-affinity analyte-specific monoclonal antibodies (MAbs). This work presents an innovative screening method, known as a bacterial macroarray, generated by contact printing of hybridoma cell supernatant samples on a nitrocellulose (NC) membrane initially coated with fluorescein isothiocyanate (FITC)-labeled bacteria. Given that bacterial fixation will be influenced by complex bacterial surface structures, we selected both gram-positive bacteria ( Staphylococcus aureus and Listeria monocytogenes) and gram-negative bacteria ( Escherichia coli O157:H7 and Cronobacter sakazakii) to optimize the fixation conditions for binding to the NC membrane, such as the aperture of the NC membrane, the concentration of bacteria, the dosage of glycerin in the spotting buffer, and the fixation time and temperature...
October 10, 2018: SLAS Discovery
Oscar Serrano, Hanan Almahasheer, Carlos M Duarte, Xabier Irigoien
Seagrasses play an important role in climate change mitigation and adaptation, acting as natural CO2 sinks and buffering the impacts of rising sea level. However, global estimates of organic carbon (Corg ) stocks, accumulation rates and seafloor elevation rates in seagrasses are limited to a few regions, thus potentially biasing global estimates. Here we assessed the extent of soil Corg stocks and accumulation rates in seagrass meadows (Thalassia hemprichii, Enhalus acoroides, Halophila stipulacea, Thalassodendrum ciliatum and Halodule uninervis) from Saudi Arabia...
October 9, 2018: Scientific Reports
Ömür Acet, Neşe Hayat Aksoy, Demet Erdönmez, Mehmet Odabaşı
In order to investigate the biocatalytic properties of α-amylase on a composite cryogel matrix with immobilized metal affinity chromatography, Cu+2 -attached poly(2-hydroxyethyl methacrylate) (Cu+2 -PHEMA) beads, (2 µm size) were synthesized, then composite cryogel column was prepared by composing beads and PHEMA cryogels. After the preparation of Cu+2 -PHEMA beads embedded cryogel column (Cu+2 -BEC), some experiments were tested. Accordingly, the highest adsorption capacity (676.8 mg/g particles) of cryogels was achieved at acetate buffer of pH 5...
October 9, 2018: Artificial Cells, Nanomedicine, and Biotechnology
Paolo A Sossi, Frédéric Moynier, Kirsten van Zuilen
Terrestrial and lunar rocks share chemical and isotopic similarities in refractory elements, suggestive of a common precursor. By contrast, the marked depletion of volatile elements in lunar rocks together with their enrichment in heavy isotopes compared with Earth's mantle suggests that the Moon underwent evaporative loss of volatiles. However, whether equilibrium prevailed during evaporation and, if so, at what conditions (temperature, pressure, and oxygen fugacity) remain unconstrained. Chromium may shed light on this question, as it has several thermodynamically stable, oxidized gas species that can distinguish between kinetic and equilibrium regimes...
October 8, 2018: Proceedings of the National Academy of Sciences of the United States of America
Octavio Roncero, Alexandre Zanchet, Alfredo Aguado
Is the rise of the rate constant measured in laval expansion experiments of OH with organic molecules at low temperatures due to the reaction between the reactants or due to the formation of complexes with the buffer gas? This question has importance for understanding the evolution of prebiotic molecules observed in different astrophysical objects. Among these molecules methanol is one of the most widely observed, and its reaction with OH has been studied by several groups showing a fast increase in the rate constant under 100 K...
October 17, 2018: Physical Chemistry Chemical Physics: PCCP
Nuno Vasco Gama, Cláudia Amaral, Tiago Silva, Romeu Vicente, João Araújo Pereira Coutinho, Ana Barros-Timmons, Artur Ferreira
The aim of this study was to enhance the thermal comfort properties of crude glycerol (CG) derived polyurethane foams (PUFs) using phase change materials (PCMs) (2.5⁻10.0% ( wt/wt )) to contribute to the reduction of the use of non-renewable resources and increase energy savings. The main challenge when adding PCM to PUFs is to combine the low conductivity of PUFs whilst taking advantage of the heat released/absorbed by PCMs to achieve efficient thermal regulation. The solution considered to overcome this limitation was to use expandable graphite (EG) (0...
October 4, 2018: Materials
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