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Tobacco use

Andriy Bilichak, Igor Kovalchuk
Virus-induced gene silencing (VIGS) is a powerful epigenetic tool that allows in a relatively short period of time to down-regulate the expression of an endogenous gene in infected plants for either monitoring the resulting phenotype or enhancing/modifying a particular trait associated with the gene. Here, we describe the utilization of Tobacco rattle virus (TRV) as a vector for the VIGS technique in Arabidopsis plants. The unique ability of TRV to infect both somatic tissues and gametes allows deciphering the role of genes in these tissues simultaneously...
2017: Methods in Molecular Biology
Kensuke Yamamura, Yoshifumi Baba, Shigeki Nakagawa, Kosuke Mima, Keisuke Miyake, Kenichi Nakamura, Hiroshi Sawayama, Koichi Kinoshita, Takatsugu Ishimoto, Masaaki Iwatsuki, Yasuo Sakamoto, Yoichi Yamashita, Naoya Yoshida, Masayuki Watanabe, Hideo Baba
PURPOSE: Fusobacterium nucleatum (F. nucleatum) is a component of the human microbiome that primarily inhabits the oral cavity. It causes periodontal disease and has also been implicated in the development of human cancers. Although there are several reports of the relationship between F. nucleatum and the clinical outcome in human cancers, its prognostic significance in esophageal cancer remains unclear. EXPERIMENTAL DESIGN: We quantified F. nucleatum DNA in 325 resected esophageal cancer specimens by qPCR...
October 21, 2016: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
Drew A Torigian, Judith Green-McKenzie, Xianling Liu, Frances S Shofer, Thomas Werner, Catherine E Smith, Andrew A Strasser, Mateen C Moghbel, Ami H Parekh, Grace Choi, Marcus D Goncalves, Natalie Spaccarelli, Saied Gholami, Prithvi S Kumar, Yubing Tong, Jayaram K Udupa, Clementina Mesaros, Abass Alavi
RATIONALE AND OBJECTIVES: The aim of this study was to assess the feasibility of (18)F-fluorodeoxyglucose (FDG)-positron emission tomography/computed tomography (PET/CT) to systematically detect and quantify differential effects of chronic tobacco use in organs of the whole body. MATERIALS AND METHODS: Twenty healthy male subjects (10 nonsmokers and 10 chronic heavy smokers) were enrolled. Subjects underwent whole-body FDG-PET/CT, diagnostic unenhanced chest CT, mini-mental state examination, urine testing for oxidative stress, and serum testing...
October 18, 2016: Academic Radiology
Gabrieli Bernardi, Magali Kemmerich, Lucila C Ribeiro, Martha B Adaime, Renato Zanella, Osmar D Prestes
An effective method has been developed and validated for the determination of residues of 55 pesticides in tobacco. The proposed sample preparation method is based on acetonitrile extraction, low-temperature precipitation (LTP) and dispersive solid phase extraction (d-SPE) clean-up. Gas chromatography and ultra-high performance liquid chromatography analysis, both coupled to tandem mass spectrometry (GC-MS/MS and UHPLC-MS/MS), were used for determination. LTP is easy to perform and was crucial to obtain a clean extract...
December 1, 2016: Talanta
Yuyan Shi, John P Pierce, Martha White, Maya Vijayaraghavan, Wilson Compton, Kevin Conway, Anne M Hartman, Karen Messer
BACKGROUND: Electronic cigarettes (e-cigarettes) are heavily marketed and widely perceived as helpful for quitting or reducing smoking intensity. We test whether ever-use of e-cigarettes among early adopters was associated with: 1) increased cigarette smoking cessation; and 2) reduced cigarette consumption. METHODS: A representative cohort of U.S. smokers (N = 2454) from the 2010 Tobacco Use Supplement to the Current Population Survey (TUS-CPS) was re-interviewed 1 year later...
October 21, 2016: BMC Public Health
Alexa A Lopez, Marzena Hiler, Sarah Maloney, Thomas Eissenberg, Alison B Breland
BACKGROUND: Novel tobacco products entering the US market include electronic cigarettes (ECIGs) and products advertised to "heat, not burn" tobacco. There is a growing literature regarding the acute effects of ECIGs. Less is known about "heat, not burn" products. This study's purpose was to expand existing clinical laboratory methods to examine, in cigarette smokers, the acute effects of a "heat, not burn" "loose-leaf tobacco vaporizer" (LLTV). METHODS: Plasma nicotine and breath carbon monoxide (CO) concentration and tobacco abstinence symptom severity were measured before and after two 10-puff (30-s interpuff interval) product use bouts separated by 60min...
October 11, 2016: Drug and Alcohol Dependence
Elizabeth M Dlugosz, Scott C Lenaghan, C Neal Stewart
Over the last decade there has been a resurgence in the use of plant protoplasts that range from model species to crop species, for analysis of signal transduction pathways, transcriptional regulatory networks, gene expression, genome-editing, and gene-silencing. Furthermore, significant progress has been made in the regeneration of plants from protoplasts, which has generated even more interest in the use of these systems for plant genomics. In this work, a protocol has been developed for automation of protoplast isolation and transformation from a 'Bright Yellow' 2 (BY-2) tobacco suspension culture using a robotic platform...
September 27, 2016: Journal of Visualized Experiments: JoVE
Mansoo Yu, Paulette E Chavez, René Olate, Clark Peters
Although Latino/Hispanic adolescent substance use is a growing research area in the United States, there is little research examining the prevalence and factors associated with adolescent cigarette smoking status in this population. A nationally representative sample of 5,929 middle and high Latino/Hispanic students in the 2009 US National Youth Tobacco Survey was selected to assess the prevalence and various risk and protective factors associated with cigarette smoking status. Results revealed one in five (20%) were experimental smokers, nearly one in eleven (8...
October 21, 2016: Substance Use & Misuse
Jasmine A Mena, Gifty G Ampadu, James O Prochaska
BACKGROUND: Tobacco use remains the leading cause of preventable disease and death. Most smokers are not motivated to quit; however, most smoking cessation interventions are designed for smokers who are ready to take action. OBJECTIVES: The aim of the present study was to describe participant engagement and satisfaction with telephonic smoking cessation coaching with a population of smokers at different stages of readiness to quit. METHODS: Qualitative description was used to capture the experiences of 62 individuals who participated in telephonic smoking cessation coaching using semistructured interviews...
October 21, 2016: Substance Use & Misuse
Benjamin H Han, Scott Sherman, Pia M Mauro, Silvia S Martins, James Rotenberg, Joseph J Palamar
BACKGROUND AND AIMS: The ageing United States (US) population is providing an unprecedented population of older adults who use recreational drugs. We aimed to estimate the trends in the prevalence of past-year use of cannabis, describe the patterns and attitudes, and determine correlates of cannabis use by adults age 50 and older. DESIGN: Secondary analysis of the National Survey on Drug Use and Health survey from 2006 to 2013, a cross-sectional survey given to a nationally representative probability sample of populations living in US households...
October 21, 2016: Addiction
Abdullah Turhan, Simone Onrust, Peter Ten Klooster, Marcel Pieterse
AIMS: To test effectiveness of the Healthy School and Drugs (HSD) program on tobacco and alcohol use in Dutch secondary special education (SE) schools, and whether this depends on subtypes of SE-schools and the level of implementation. DESIGN: In a quasi-experimental design with baseline and post-treatment follow-up 35 classes (N = 363) were allocated arbitrarily or depending on teacher motivation to either intervention condition (N = 205) or usual curriculum (N = 158)...
October 21, 2016: Addiction
Debjani S Goswami, Tanuja P Manohar
INTRODUCTION: Cardiovascular diseases are becoming the major cause of morbidity and mortality in most of the developing countries including India. Cardiometabolic risk factors play a vital role in causation of coronary artery disease. Present study was carried out to investigate the role of various risk factors in the occurrence of coronary artery disease (CAD) in population from Central India. SETTING AND DESIGN: This was a hospital-based age and sex matched case-control study, carried out at Lata Mangeshkar Hospital, Nagpur...
October 2016: Journal of the Association of Physicians of India
Jack E Henningfield, Tracy T Smith, Bethea A Kleykamp, Reginald V Fant, Eric C Donny
BACKGROUND AND RATIONALE: Steven R. Goldberg was a pioneering behavioral pharmacologist whose intravenous drug self-administration studies advanced the understanding of conditioned stimuli and schedules of reinforcement as determinants of pattern and persistence of drug-seeking behavior, and in particular, the importance of nicotine in tobacco use. His passing in 2014 led to invitations to contribute articles to psychopharmacology dedicated to his work. OBJECTIVES: The objectives of this review are to summarize and put into historical perspective Goldberg's contributions to elucidate the reinforcing effects of nicotine and to summarize the implications of his research for medication development, tobacco regulation, and potential tobacco control policy options...
October 21, 2016: Psychopharmacology
Humberto Parada, Patrick T Bradshaw, Lawrence S Engel, Kathleen Conway, Susan E Steck, Susan L Teitelbaum, Alfred I Neugut, Regina M Santella, Marilie D Gammon
BACKGROUND: Environmental tobacco smoke (ETS) exposure is hypothesized to influence survival after breast cancer, but few studies have examined this association. METHODS: A population-based cohort of women (N=1,508) diagnosed with first primary invasive or in situ breast cancer in 1996-1997 was interviewed shortly after diagnosis and again approximately 5 years later to assess ETS exposure, and women were followed for over 18 years using the National Death Index; 597 deaths (237 associated with breast cancer) were identified...
October 7, 2016: Cancer Epidemiology, Biomarkers & Prevention
S Charntikov, A M Falco, K Fink, L P Dwoskin, R A Bevins
Nicotine is the primary addictive component of tobacco products and its complex stimulus effects are readily discriminated by human and non-human animals. Previous research with rodents directly investigating the nature of the nicotine stimulus has been limited to males. The current study began to address this significant gap in the literature by training female and male rats to discriminate 0.4 mg/kg nicotine from saline in the discriminated goal-tracking task. In this task, access to sucrose was intermittently available on nicotine session...
October 17, 2016: Neuropharmacology
Peter N Lee, Alison J Thornton, Barbara A Forey, Jan S Hamling
OBJECTIVES: The study aimed to review the epidemiological evidence relating environmental tobacco smoke exposure to stroke in never smokers. METHODS: The study is similar to our review in 2006, with searches extended to March 2016. RESULTS: Twelve further studies were identified. A total of 28 studies varied considerably in design, exposure indices used, and disease definition. Based on 39 sex-specific estimates and the exposure index current spousal exposure (or nearest equivalent), the meta-analysis gave an overall fixed-effect relative risk estimate of 1...
October 17, 2016: Journal of Stroke and Cerebrovascular Diseases: the Official Journal of National Stroke Association
Yuqin Wang, Baochan Wang, Shiyan Yan, Feng Shen, Haixia Cao, Jiangao Fan, Rongrong Zhang, Jun Gu
PURPOSE: Questions remain uncertainty regarding the gender differences in the relationship between type 2 diabetes (T2DM) and liver cancer risk. By considering several confounding factors, we aimed to identify this issue according to a meta-analysis of cohort studies. METHODS: We searched EMBASE and MEDLINE for studies on the association between T2DM and risk of liver cancer up to November 30, 2014. A random-effects model was performed to calculate summary relative risks (SRRs) with corresponding 95% confidence intervals (CIs)...
September 22, 2016: Annals of Epidemiology
Priyanka G Bhosale, Manishkumar Pandey, Rajiv S Desai, Asawari Patil, Shubhada Kane, Kumar Prabhash, Manoj B Mahimkar
OBJECTIVES: In the present study, we comprehensively analyzed the prevalence of transcriptionally active human papilloma virus (HPV) in tissue samples of Indian patients with leukoplakia, predominantly hyperplastic lesions and head and neck squamous cell carcinoma (HNSCC). In addition, saliva samples from patients with HNSCC were screened for HPV detection. STUDY DESIGN: P16 overexpression was analyzed by immunohistochemistry. Tissue samples of leukoplakia (n = 121) and HNSCC (n = 427) and saliva from patients with HNSCC (n = 215) were tested for HPV using nested polymerase chain reaction...
November 2016: Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology
Ida Steen, Sandra S Waehrens, Mikael A Petersen, Morten Münchow, Wender L P Bredie
The present study aimed to investigate coffee flavour perception and release as function of serving temperature to support standardisation in the specialty coffee branch. The coffee cultivar Bourbon Caturra was evaluated at six serving temperatures ranging from 31°C to 62°C. Coffee samples were analysed by dynamic headspace sampling gas chromatography-mass spectrometry and descriptive analyses using sip-and-spit tasting. The release of volatiles followed mostly the van't Hoff principle and was exuberated at temperatures above 40°C...
March 15, 2017: Food Chemistry
Jens Christoffer Skogen, Tormod Bøe, Børge Sivertsen, Mari Hysing
OBJECTIVE: To describe potential differences in unhealthy behaviours among ethnic Norwegian adolescents and minority adolescents from countries within the European Union, European Economic Area or US (EU/EEA countries) and adolescents from non-EU/EEA countries. Specifically, we aimed to investigate ethnic differences in use of alcohol, tobacco and illicit drugs, and potential confounding due to socio-demographic characteristics. DESIGN: Cross-sectional population-based study of adolescents aged 16-19 (N = 10,122), with self-reported ethnicity as grouping variable, and self-reported use of alcohol, tobacco and illicit drugs as dependent variables...
October 21, 2016: Ethnicity & Health
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