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Ya-Jun Zhai, Hui Huang, Jianhua Liu, Hua-Run Sun, Dandan He, Yu-Shan Pan, Gongzheng Hu
Background: Colistin has been used as the last therapeutic resort for treatment of MDR Gram-negative bacteria infections in humans. The two-component system CpxAR has been reported to contribute to the MDR of bacteria. There may be a more complex network mediated by CpxAR contributing to colistin susceptibility than previously understood. Methods: A series of AcrB or CpxR deletion mutants of a multidrug-susceptible standard strain of Salmonella enterica serovar Typhimurium (Salmonella Typhimurium) was constructed in our previous study...
August 10, 2018: Journal of Antimicrobial Chemotherapy
Shujun Ou, Jinfeng Chen, Ning Jiang
Assembling a plant genome is challenging due to the abundance of repetitive sequences, yet no standard is available to evaluate the assembly of repeat space. LTR retrotransposons (LTR-RTs) are the predominant interspersed repeat that is poorly assembled in draft genomes. Here, we propose a reference-free genome metric called LTR Assembly Index (LAI) that evaluates assembly continuity using LTR-RTs. After correcting for LTR-RT amplification dynamics, we show that LAI is independent of genome size, genomic LTR-RT content, and gene space evaluation metrics (i...
August 10, 2018: Nucleic Acids Research
Xin Sui, Shijian Deng, Mengmeng Liu, Linlin Fan, Yunfei Wang, Huaxing Xu, Yao Sun, Anil Kishen, Qi Zhang
BACKGROUND/AIMS: Activation of the Wnt/β-catenin signalling pathway has been widely investigated in bone biology and shown to promote bone formation. However, its specific effects on osteoclast differentiation have not been fully elucidated. Our study aimed to identify the role of β-catenin in osteoclastogenesis and bone homeostasis. METHODS: In the present study, exon 3 in the β-catenin gene (Ctnnb1) allele encoding phosphorylation target serine/threonine residues was flanked by floxP sequences...
August 14, 2018: Cellular Physiology and Biochemistry
Meizhi Wang, Hui Gao, Haijun Qu, Jing Li, Kaili Liu, Zhiwu Han
BACKGROUND: The most frequent type of renal cell carcinoma is called clear-cell renal cell carcinoma (ccRCC) which is associated with a poor prognosis. It has been observed that miR-137 is aberrantly expressed in many different kinds of human malignancies including ccRCC. This research aims to examine the role of miR-137 in ccRCC. METHODS: Quantitative RT-PCR (qRT-PCR) was applied to measure miR-137 expression in ccRCC and adjacent noncancerous tissue. Gene expression was determined by western blot...
April 22, 2018: Pharmacological Reports: PR
Shuang Liang, Jia-Ming Zhang, Zheng-Tao Lv, Peng Cheng, Wen-Tao Zhu, An-Min Chen
SKT11, an important tumor suppressor, is a member of the serine/threonine kinase family and plays a crucial role in tumor invasion and metastasis by activated adenine monophosphate-activated protein kinase (AMPK) and AMPK-related kinase proteins. However, few studies have elaborated its regulations of development and metabolism of cartilage, as well as skeleton. This study was aimed to investigate the role of Stk11-knockout in chondrocyte by bioinformatics analysis. The gene expression profiles for Stk11-knockout and wild-type mice were downloaded from the Gene Expression Omnibus (GEO) database...
August 11, 2018: Gene
Li Ding, Xu Wang, Xiaoli Guo, Shugeng Chen, Hewei Wang, Ning Jiang, Jie Jia
Mirror visual feedback (MVF) is used widely for motor recovery after stroke, but an optimal training setup and systematic procedure are lacking. New optimization strategies have been proposed, one of which is a camera technique. We investigated the effects of a camera-based MVF setup on motor function and motor processes upstream for upper-limb rehabilitation. Seventy-nine stroke patients were assigned randomly to the MVF group (MG; N = 38) or conventional group (CG; N = 41), which respectively received camera-based MVF and dosage-equivalent physiotherapy or/and occupational therapy for 1 h/day and 5 days/week for 4 weeks...
August 13, 2018: IEEE Transactions on Neural Systems and Rehabilitation Engineering
Pengfei Qiao, Shenglong Li, Haogang Zhang, Lei Yao, Fujing Wang
Colorectal cancer (CRC) is among the most common malignancies of the digestive system. Dysregulation of miRNAs and the farnesoid X receptor (FXR) are involved in the progression of CRC. In the present study, the effects of FXR and miR‑135A1 in CRC were evaluated. Reverse transcription quantitative‑polymerase chain reaction (RT‑qPCR) was used to examine the expression of miR‑135A1 in patient CRC tissues and adjacent non‑tumor tissues, as well as cell lines. The association between miR‑135A1 and clinical characteristics of patients with CRC was also investigated...
August 7, 2018: Oncology Reports
Zhong Qiao, Yinyin Ye, Pin Hsuan Chang, Devibaghya Thirunarayanan, Krista R Wigginton
Determining the influence of higher order structure on UVC photolysis will help inform predictions of nucleic acid fate and microorganism inactivation. We measured the direct UV254 photolysis kinetics of four model viral genomes composed of single-stranded and double-stranded RNA (ssRNA and dsRNA, respectively), as well as single-stranded and double-stranded DNA (ssDNA and dsDNA, respectively), in ultrapure water, in phosphate buffered saline (PBS), and encapsidated in their native virus particles. The photolysis rate constants of naked nucleic acids measured by qPCR (RT-qPCR for RNA) and normalized by the number of bases measured in a particular sequence exhibited the following trend: ssDNA > ssRNA ≈ dsDNA > dsRNA...
August 14, 2018: Environmental Science & Technology
Lu Shi, Ruowen Xiao, Menghe Wang, Meiyin Zhang, Nuoqing Weng, Xinge Zhao, X F Steven Zheng, Huiyun Wang, Shijuan Mai
Deregulated microRNAs play an important role in the development and progression of various types of cancer. In our previous study, we observed that microRNA‑342‑3p (miR‑342‑3p) was one of the most markedly downregulated microRNAs in two nasopharyngeal carcinoma (NPC) cell lines compared to non‑neoplastic cells by using whole genome small RNA sequencing. In the present study, we confirmed that the expression of miR‑342‑3p was significantly reduced in NPC tissues compared with normal nasopharyngeal epithelial tissues...
August 10, 2018: Oncology Reports
Erjie Xia, Adheesh Bhandari, Yanyan Shen, Xiaofen Zhou, Ouchen Wang
The incidence of thyroid cancer has increased in the past decades; however, the underlying molecular mechanisms of thyroid cancer tumorigenesis remain unknown. Using sequencing technology, long intergenic non‑protein coding RNA 673 (LINC00673) was identified to be upregulated in several tumor tissues. However, the biological role of LINC00673 in thyroid carcinoma has yet to be determined. In this study, 60 matched pairs of thyroid tumor tissue and normal tissue were selected for study using reverse transcription-quantitative polymerase chain reaction (RT-qPCR) to validate previous findings; then, clinicopathologic features of the tissues were analyzed...
August 14, 2018: International Journal of Oncology
Dingyuan Luo, Xinke Xu, Junliang Li, Cheng Chen, Wei Chen, Fangyu Wang, Yanping Xie, Fangcheng Li
Glioblastoma multiforme (GBM) is the most common primary malignant tumor affecting the human brain. Despite improvements in therapeutic technologies, patients with GBM have a poor clinical result and the molecular mechanisms responsible for the development of GBM have not yet been fully elucidated. 3-phosphoinositide dependent protein kinase 1 (PDK1) is upregulated in various tumors and promotes tumor invasion. In glioma, transforming growth factor-β (TGF‑β) promotes cell invasion; however, whether TGF‑β directly regulates PDK1 protein and promotes proliferation and invasion is not yet clear...
August 14, 2018: International Journal of Oncology
Na Zhou, Zhao Sun, Ningning Li, Yuping Ge, Jianfeng Zhou, Qin Han, Lin Zhao, Chunmei Bai
The incidence and mortality of colorectal cancer (CRC) have been rising rapidly in China. A number of miRNAs have been confirmed to be involved in diverse biological processes of CRC. However, whether miR‑197 plays a role in migration and invasion of CRC has never been explored. In the present study Transwell chambers were used in in vitro migration and invasion assays. Dual-luciferase reporter assay was employed to confirm the target of miR‑197. RT‑PCR and IHC staining were performed to quantify miR‑197 and IGFBP3 expression, respectively...
August 10, 2018: Oncology Reports
Jiangtao Zhou, Yina Zhao, Guangwen Wu, Bingbing Lin, Zuanfang Li, Xianxiang Liu
The differential microRNA (miRNA) omics of the synovial membrane were investigated using a rat model of knee osteoarthritis (KOA) induced by bilateral anterior cruciate ligament transection, which produced pathological biomarkers in KOA. Sprague‑Dawley rats were randomly divided into two groups; Sham‑operated and KOA‑operated group. The KOA rats were subjected to bilateral anterior cruciate ligament transection. After 6 weeks, total RNA was extracted from the knee joint synovial membrane of the rats and a microRNA (miR) microarray was performed to identify differentially expressed miRs...
August 13, 2018: Molecular Medicine Reports
Ruixing Liu, Xiaoming Yan
The aim of the present study was to examine whether activation of the nuclear factor E2‑related factor 2 (Nrf‑2)/heme oxygenase‑1 (HO‑1) antioxidant pathway in the cornea was involved in the protective effect of sulforaphane (SF) following keratoconus (KC) injury. Following epithelial debridement, collagenase type II was applied in KC groups at room temperature for 30 min. Following this, rabbits were administered with a subconjunctival (s.c.) injection of SF or placebo (maize oil) daily for a total of 2 weeks...
August 10, 2018: International Journal of Molecular Medicine
Xiaobo Hui, Shiming Zhang, Yanping Wang
MicroRNAs (miRNA/miRs) serve crucial roles in the progression of human glioblastoma (GBM); however, the exact regulatory mechanisms of miRNAs in human GBM remain unclear. The present study aimed to investigate the roles of miR‑454‑3p in human GBM. Reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) analysis was performed to examine the expression of miR‑454‑3p in glioma tissues and adjacent tissues. Human GBM cell lines (LN‑229, A172 and GL15) and a normal human astrocyte cells (HA1800) were used for analysis...
August 14, 2018: Molecular Medicine Reports
Wei Zhang, Jian Feng, Biao Cheng, Qing Lu, Xiaoping Chen
Oxidative injury of vascular endothelial cells in the initial event of atherosclerosis (AS) in diabetes was assessed in the present study. The antioxidant effect of oleanolic acid (OA) has attracted much attention. In the present study the potential effects of OA on human umbilical vein endothelial cells (HUVECs) were investigated. Cell viability was examined using the CCK‑8 assay. The activity of oxidative stress parameters was determined using commercial kits. Flow cytometry analysis was performed to detect the level of reactive oxygen species (ROS), mitochondrial membrane potential (MMP) and cell apoptosis...
August 3, 2018: Molecular Medicine Reports
Eirini Vasarmidi, Stella Sarantoulaki, Athina Trachalaki, George Margaritopoulos, Eleni Bibaki, Demetrios A Spandidos, Nikolaos Tzanakis, Katerina Antoniou
Idiopathic pulmonary fibrosis (IPF) is a chronic and irreversible interstitial lung disease with a poor prognosis and limited therapeutic options. Over the past decade, research efforts have focused on the pathogenetic mechanisms involved in this enigmatic lung disease. Rheumatoid arthritis (RA) is a chronic inflammatory autoimmune disease often complicated by the development of interstitial lung disease (ILD), leading to high mortality and morbidity. Autophagy is a process regulating the turnover of subcellular components and organelles, and represents a major cellular homeostatic mechanism...
August 6, 2018: Molecular Medicine Reports
Cai-Yun Long, Xiao-Bing Tang, Wei-Lin Wang, Zheng-Wei Yuan, Yu-Zuo Bai
Anorectal malformations (ARMs) are one of the most common congenital malformations of the digestive tract; however, the pathogenesis of this disease remains to be fully elucidated. MicroRNAs (miRNAs) are important in gastrointestinal development and may be involved in the pathogenesis of ARMs. The present study aimed to profile miRNAs and examine their potential functions in rats with ethylenethiourea (ETU)‑induced ARMs. Pregnant Wistar rats (n=36) were divided randomly into ETU‑treated and control groups...
August 7, 2018: International Journal of Molecular Medicine
Ravi K Chilukoti, Josefine Lendeckel, Katrin Darm, Alicja Bukowska, Andreas Goette, Marc Sühling, Kirsten Utpatel, Barbara Peters, Georg Homuth, Uwe Völker, Carmen Wolke, Christian Scharf, Uwe Lendeckel
Dronedarone improves microvascular flow during atrial fibrillation and reduces the infarct size in acute models of myocardial infarction. However, dronedarone might be harmful in patients with recent decompensated heart failure and increases mortality in patients with permanent atrial fibrillation. A pathophysiological explanation for these discrepant data is lacking. This study investigated the effects of dronedarone on gene and protein expression in the infarcted area and border zone in pigs subjected to anterior ischemia/reperfusion myocardial infarction...
July 2018: Experimental Biology and Medicine
Thomas Hendrix Beckham, Paul Bernard Romesser, Andries Hijlke Groen, Christopher Sabol, Ashok R Shaha, Mona Sabra, Thomas Brinkman, Daniel J Spielsinger, Sean McBride, Jillian Tsai, Nadeem Riaz, R Michael Tuttle, James A Fagin, Eric J Sherman, Richard J Wong, Nancy Y Lee
BACKGROUND: Differentiated thyroid cancer typically has an indolent clinical course but can cause significant morbidity by local progression. Surgery can be difficult due to the proximity to critical normal structures in the neck. Our objective was to review the safety, feasibility, and outcomes of definitive-intent intensity modulated radiation therapy (IMRT) and to analyze whether patients receiving concurrent chemotherapy (CC-IMRT) had higher rates of disease control and survival over IMRT alone in patients with unresectable or gross residual disease (GRD)...
August 14, 2018: Thyroid: Official Journal of the American Thyroid Association
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