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Anastas Krassenov Demirev, Irena Dimitrova Kostadinova, Iliya Rumenov Gabrovski
Objectives: Radiation pneumonitis (RP) can be an adverse complication of radiotherapy (RT) and can limit the application of the already planned radiation dose. It is often associated with RT of lung carcinoma and is occasionally caused by radiation therapy of breast carcinoma and lymphomas located in the mediastinum. Positron emission tomography/computed tomography (PET/CT) emerges lately as a prospective modality for early diagnostics of RP. The aim of this study was to summarize the initial data from diagnostic application of PET/CT in patients suspicious of RP and to derive criteria, which can help differentiate RP from early recurrence of the disease and/or residual tumor...
October 9, 2018: Molecular Imaging and Radionuclide Therapy
F Q Zhou, D K He, Y R Shao, J Shen
Objective: To investigate the effects of methylprednisolone on NOD-like receptor hot protein domain-associated protein 3 (NLRP3) inflammasome in phosgene-induced acute lung injury. Methods: Rats were randomly divided into four groups, 10 rats in Air group (inhalation of air of the same volume as the phosgene group) , 10 rats in Phosgene group (inhalation of 8.33 mg/L with 100% purity phosgene for 5 min) , 10 rats in Saline control group (inhalation of the same dose of phosgene and 2 mg/kg saline via tail vein injection one hour later) , 10 rats in MP group (inhalation of the same dose of phosgene and 2 mg/kg MP via tail vein injection one hour later) ...
August 20, 2018: Chinese Journal of Industrial Hygiene and Occupational Diseases
C D Zhu, M Z Guo, Q Cai, Y Y Li, K X Wu, M Huang
Objective: To investigate the roles of p38 mitogen-activated protein kinases (p38 MAPK) , extracellular regulated protein kinases (ERK) and c-Jun N-tenninal kinases (JNK) of MAPK signaling pathway in Paraquat-induced epithelial to mesenchymal transition (EMT) of type II alveolarepithelial cells. Methods: RLE-6NT cells were incubated with different concentrations of PQ (0, 25, 50, 100μmol/L) for 6, 12 and 24 h. Cell morphology alteration was observed under phase-contrast microscopy. Cell viability was determined using an MTT assay...
August 20, 2018: Chinese Journal of Industrial Hygiene and Occupational Diseases
L X Ji, J Liu
Objective: MicroRNA-221/222 is involved in the pathogenesis of intrahepatic cholestasis of pregnancy (ICP) to promote the apoptosis of placental bile acids through human trophoblastic cells. This study investigates the effects of miR-221/222 on proliferation, apoptosis and apoptosis-related proteins of human trophoblast HTR-8/SVneo (HTR-8 cells) to understand its role in promoting trophoblastic apoptosis. Methods: The experiment was divided into transfection group and negative control group. Transient transfection method was used in both groups...
August 20, 2018: Zhonghua Gan Zang Bing za Zhi, Zhonghua Ganzangbing Zazhi, Chinese Journal of Hepatology
R Qi, C G Lei, Y X Bai, X Xing
Objective: To study the effect and mechanism of angiotensin (Ang II) on the proliferation of human hepatocellular carcinoma HepG2 cells. Methods: The effects of different concentrations of Ang II's (10(-8)-10(-4) mol/L) on proliferated hepatocellular carcinoma HepG2 cells were detected by CCK-8 assay. The expression of angiotensin II type 1 receptor (AT1) protein and activation of ERK1/2 protein in hepatocellular carcinoma HepG2 cells after processing with Ang II were assayed by Western blot. The cells were pretreated with candesartan (AT1 receptor antagonist), sorafenib (Raf kinase inhibitor) and PD98059 (ERK1/2 inhibitor) for 1...
August 20, 2018: Zhonghua Gan Zang Bing za Zhi, Zhonghua Ganzangbing Zazhi, Chinese Journal of Hepatology
Xin-Yue Lian, Ji-Chun Ma, Jing-Dong Zhou, Ting-Juan Zhang, De-Hong Wu, Zhao-Qun Deng, Zhi-Hui Zhang, Xi-Xi Li, Pin-Fang He, Yang Yan, Jiang Lin, Jun Qian
The current study was aimed to investigate integrin beta-like 1 (ITGBL1) methylation pattern and its clinical relevance in patients with acute myeloid leukemia (AML). Real-time methylation-specific polymerase chain reaction (PCR; RQ-MSP) and bisulfite sequencing PCR (BSP) were performed to detect the methylation of ITGBL1 promoter. Real-time quantitative PCR (RT-qPCR) was performed to analyze ITGBL1 transcript level. The results showed that ITGBL1 methylation level in 131 patients with AML was significantly higher than 29 controls (p < 0...
October 14, 2018: Journal of Cellular Physiology
Jalal Choupani, Mohammad R Alivand, Sima M Derakhshan, Mohammad Zaeifizadeh, Mahmoud S Khaniani
Distinct metastasis is one of the main causes of breast cancer (BC)-related mortality and epithelial-mesenchymal transition (EMT) is a primary step in metastasis dissemination. On the other hand, doxorubicin (DOX) is an effective chemotherapeutic agent against BC; unfortunately, its clinical use is limited by dose-dependent side effects. Therefore, extensive efforts have been dedicated to suppressing metastasis of BC and also to overcome DOX side effects together with keeping its antitumor efficacy. Studies supported the role of oleuropein (OLEU) in reducing DOX-induced side effects besides its antitumor actions...
October 14, 2018: Journal of Cellular Physiology
Lizhen Zhu, Qi Zhu, Huihuang Wen, Xiang Huang, Ge Zheng
BACKGROUND: In this study, we aimed to explore the effects of GAS5 single-nucleotide polymorphisms (SNPs) on GAS5 expression. And the signaling pathways underlying the function of GAS5 during the pathogenesis of prostate cancer (PC) were clarified. MATERIALS AND METHODS: Patients with PC were recruited and grouped according to their specific genotypes of rs55829688 and rs145204276. Kaplan-Meier overall survival curves were calculated and compared among different groups...
October 14, 2018: Journal of Cellular Physiology
Naishi Zhao, Jianfeng Zhang
Vascular endothelial cell growth factor A (VEGF-A) signaling promotes the endothelial cell proliferation, macrophage infiltration and foam cell formation, which play pivotal roles in the pathogenesis of atherosclerosis (AS). However, the role of alternative splicing of VEGF here is not known. Here, ApoE (-/-) mice supplied high-fat diet (HFD mice) were used to generate AS, while ApoE (-/-) mice supplied with normal diet (NOR mice) were used as a control. Aortic endothelial cells (AECs) and infiltrated macrophages were purified and quantified by flow cytometry...
October 13, 2018: Aging
Anat Achiron, Rina Falb, Anna Feldman, Maria Bovim, Onn Rosenblum, Ida Sarova Pinhas, David Magalashvili, Mark Dolev, Shay Menascu, Michael Gurevich
BACKGROUND: The operating molecular mechanisms that characterize a multiple sclerosis (MS) relapse has not been thoroughly studied. We have shown that increased expression of RNA polymerase 1 (POL1) molecular pathway is associated with increased MS disease activity. OBJECTIVE: To assess POL1 pathway expression during acute MS relapse. METHODS: We studied POL1 pathway activation and associated biomarkers during the first acute optic neuritis attack of MS, and in relapsing-remitting MS patients treated with disease-modifying drugs (DMDs) experiencing an acute MS relapse or a radiological relapse using gene expression microarrays and quantitative RT-PCR...
October 11, 2018: Autoimmunity Reviews
Yu-Ying Lian, Hong-Hui He, Chao-Zheng Zhang, Xin-Cang Li, Yi-Hong Chen
Matrix metalloproteinases (MMPs) contribute to both normal and pathological tissue remodeling. They act as regulatory molecules by working in enzyme cascades as well as processing matrix proteins, cytokines, growth factors and adhesion molecules to generate fragments with biological effects. So MMPs could play distrinct roles in the process of pathogen infection. In present study, we cloned a MMP-2 (LvMMP-2) gene from Litopenaeus vannamei. LvMMP-2, highly expressed in epidermis, located to endoplasmic reticulum in S2 cells...
October 11, 2018: Fish & Shellfish Immunology
Andrew Buskard, Brian Zalma, Nicholes Cherup, Catherine Armitage, Craig Dent, Joseph F Signorile
BACKGROUND: Periodization is a systematic training calendar designed to provide variations in performance targeting, while maximizing results and reducing the potential for overtraining. When provided across multiple weeks, termed a mesocycle, it may also incorporate active recovery periods using specified drills designed to translate neuromuscular gains into targeted functional abilities. There are a number of models that can be used when applying periodization to resistance training (RT)...
October 11, 2018: Experimental Gerontology
Jules Lansu, Jan Groenewegen, Frits van Coevorden, Winan van Houdt, Alexander C J van Akkooi, Hester van Boven, Michiel van de Sande, Marcel Verheij, Rick L Haas
AIMS: The purpose of the study was to investigate the time dependent dynamics of wound complications and local control after preoperative radiotherapy (RT) in Extremity Soft Tissue Sarcomas (ESTS). PATIENTS & METHODS: In this retrospective cohort study, all patients treated for an extremity sarcoma with pre-operative radiotherapy followed by surgery were identified from a prospectively maintained database. A wound complication (WC) was defined as any local complication of the surgical area requiring intervention, hospital readmission or significant extension of the initial admission period...
October 6, 2018: European Journal of Surgical Oncology
X Chen, L Liu, W Nie, R Deng, J Li, Q Fu, J Fei, C Wang
BACKGROUND: Refractory infectious wounds on renal transplantation (RT) recipients significantly prolong hospital stay, increase medical costs, and threaten allograft survival. Vacuum sealing drainage (VSD) therapy is a new technique for managing wounds based on the principle of application of controlled negative pressure. The aim of this study was to summarize the efficacy and safety of VSD therapy in the management of refractory infectious wounds following RT. MATERIALS AND METHODS: This is a retrospective study of a cohort of 661 consecutive patients who received renal transplants over a period of 3 years in which the data were collected and analyzed retrospectively...
October 2018: Transplantation Proceedings
Chunyan Li, Xuejun Ma, Ziqiang Pan, Fangfang Lv, Zuguang Xia, Kai Xue, Qunling Zhang, Dongmei Ji, Junning Cao, Xiaonan Hong, Ye Guo
The standard treatment of waldeyer's ring DLBCL remains controversial. This retrospective study was designed to evaluate the role of consolidation radiotherapy (RT) in patients with stage I/II diffuse large B-cell lymphoma (DLBCL) limited in Waldeyer's ring (WR). We included 72 patients, 42 were treated with immunochemotherapy alone (CT group) and 30 were treated with immunochemotherapy followed by radiotherapy (CT + RT group). All patients received at least 3 cycles of R-CHOP regimen and achieved complete remission (CR) after immunochemotherapy...
September 24, 2018: Leukemia Research
Xiaohong Zhao, Xianwei Zou, Qian Li, Xu Cai, Liya Li, Jinren Wang, Yao Wang, Chen Fang, Fa Xu, Yun Huang, Benke Chen, Jintian Tang, Huiguo Wang
The inflammatory cellular model of RAW264.7 cells induced by lipopolysaccharide (LPS) has always been used to investigate the effect of anti-inflammatory agents in vitro. In the present study, the anti-inflammatory activity of total flavones extracted from the fermentation broth of the co-culture of Coprinus comatus and Morchella esculenta (MCF-F), and its potential molecular mechanism in LPS-challenged RAW264.7 macrophage cells were investigated. The data revealed that MCF-F exhibited anti-inflammatory activity in LPS-stimulated RAW264...
October 10, 2018: Microbial Pathogenesis
Yuxia Zou, Limin Peng, Shenda Weng, Dongdong Liang, Zhaofei Fan, Zhihao Wu, Xungang Tan, Shuang Jiao, Feng You
Androgens are critical hormones that regulate sex differentiation, sexual maturation, and spermatogenesis in vertebrates, which is mainly mediated by androgen receptors (ARs). Reports on transcript variants of ar (AR encoding gene) in human are almost always associated with cancers and androgen insensitivity syndrome. However, the knowledge of ar variants in teleosts is scarce. In this study, arβ and two transcript variants of arα (arα1 and arα2) in olive flounder (Paralichthys olivaceus) were cloned and analyzed...
October 10, 2018: Gene
Maxime Pichon, Caroline Picard, Bruno Simon, Alexandre Gaymard, Cécile Renard, Bruno Massenavette, Christophe Malcus, Guillaume Monneret, Florence Morfin-Sherpa, Martine Valette, Etienne Javouhey, Gilles Millat, Bruno Lina, Laurence Josset, Vanessa Escuret
INTRODUCTION: A child with severe combined immunodeficiency (SCID) had an influenza A(H1N1)pdm09 infection with viral excretion longer than 6 months, during 2013-2014 influenza season, despite cord blood transplantation and antiviral treatments. METHODS: Conventional real-time RT-PCR methods were used to estimate viral load and to detect the presence of the common N1 neuraminidase (NA) H275Y substitution responsible for oseltamivir resistance. Next-generation sequencing (NGS) of influenza viruses was performed retrospectively to characterize viral quasispecies in specimens...
October 10, 2018: Antiviral Research
Wenbin Wang, Xingyang Zhu, Xiaolong Du, Aman Xu, Xiao Yuan, Yanqing Zhan, Mulin Liu, Shuanhu Wang
Venous thromboembolism (VTE), encompassing deep venous thrombosis (DVT) and pulmonary embolism (PE), is the third most common cardiovascular disease. miR-150 is one of important microRNAs which play critical role in various cellular function such as endothelial progenitor cells (EPCs). In this study, we investigate the effect of miR-150 on EPCs function ex vivo and thrombus resolution in vivo. We determined miR-150 expression in EPCs isolated from DVT patients and control subjects by RT-PCR. Potential target of miR-150 was confirmed by bioinformatics analysis and luciferase reporter respectively...
October 10, 2018: Microvascular Research
A Kaveh, E Merat, S Samani, S Danandeh, S Soltan Nezhad
Abortion in dairy cattle is generally defined as the loss of fetus on days 47-265 of pregnancy and can significantly reduce the income and capital gain of farmers and producers. Therefore, sufficient economical support and preventive measurements are essential for this issue. In this study, genetic materials were extracted from the abomasum rennet, as well as homogenous preparations of brain, kidney, spleen, liver, and lung tissues of 128 aborted fetuses. Afterward, polymerase chain reaction (PCR) and Reverse Transcription Polymerase Chain Reaction (RT-PCR) tests were performed to identify the bovine viral diarrhea virus (BVDV), bovine herpesvirus 1 (BHV-1), Neospora caninum parasite, and serovars of Leptospira species...
December 2017: Archives of Razi Institute
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