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https://www.readbyqxmd.com/read/30090807/ion-exchange-chromatography-coupled-with-dynamic-coating-capillary-electrophoresis-for-simultaneous-determination-of-tropomyosin-and-arginine-kinase-in-shellfish
#1
Linglin Fu, Jinru Zhou, Chong Wang, Xiaohui Li, Lei Zheng, Yanbo Wang
Tropomyosin (TM) and arginine kinase (AK) are known as two major allergens in seafood. For the first time, we demonstrate a newly developed ion-exchange chromatography coupled with dynamic coating capillary electrophoresis (IEC-DCCE) method to simultaneously analyze the TM and AK in shellfish. First, we have optimized the procedure of IEC for simple enrichment of TM and AK crude extract. By using 30 mM borate-borax at pH 9.0 with 0.3% (v/v) Tween-20 as a dynamic coating modifier for capillary electrophoresis (CE) separation, the migration time, separation efficiency and electrophoretic resolution greatly improved...
2018: Frontiers in Chemistry
https://www.readbyqxmd.com/read/30021001/phylogenetic-analysis-of-viruses-in-tuscan-vitis-vinifera-sylvestris-gmeli-hegi
#2
Erika Sabella, Roberto Pierro, Andrea Luvisi, Alessandra Panattoni, Claudio D'Onofrio, Giancarlo Scalabrelli, Eliana Nutricati, Alessio Aprile, Luigi De Bellis, Alberto Materazzi
The health status of the native grapevine Vitis vinifera subsp. sylvestris (Gmeli) Hegi in natural areas in Europe has received little attention. A survey was carried out on wild grapevines in Tuscany (Italy), where isolates of the Grapevine rupestris stem pitting virus (GRSPaV), Grapevine leafroll-associated virus 1 and 3 (GLRaV-1 and GLRaV-3) and Grapevine virus A (GVA) were detected. The complete coat protein (CP) region of these isolates was sequenced to investigate the relationship of the viral variants from Tuscan wild grapevines with isolates from different geographical origins...
2018: PloS One
https://www.readbyqxmd.com/read/30013589/differential-shape-of-geminivirus-mutant-spectra-across-cultivated-and-wild-hosts-with-invariant-viral-consensus-sequences
#3
Sonia Sánchez-Campos, Guillermo Domínguez-Huerta, Luis Díaz-Martínez, Diego M Tomás, Jesús Navas-Castillo, Enrique Moriones, Ana Grande-Pérez
Geminiviruses (family Geminiviridae ) possess single-stranded circular DNA genomes that are replicated by cellular polymerases in plant host cell nuclei. In their hosts, geminivirus populations behave as ensembles of mutant and recombinant genomes, known as viral quasispecies. This favors the emergence of new geminiviruses with altered host range, facilitating new or more severe diseases or overcoming resistance traits. In warm and temperate areas several whitefly-transmitted geminiviruses of the genus Begomovirus cause the tomato yellow leaf curl disease (TYLCD) with significant economic consequences...
2018: Frontiers in Plant Science
https://www.readbyqxmd.com/read/30013119/complex-silica-composite-nanomaterials-templated-with-dna-origami
#4
Xiaoguo Liu, Fei Zhang, Xinxin Jing, Muchen Pan, Pi Liu, Wei Li, Bowen Zhu, Jiang Li, Hong Chen, Lihua Wang, Jianping Lin, Yan Liu, Dongyuan Zhao, Hao Yan, Chunhai Fan
Genetically encoded protein scaffolds often serve as templates for the mineralization of biocomposite materials with complex yet highly controlled structural features that span from nanometres to the macroscopic scale1-4 . Methods developed to mimic these fabrication capabilities can produce synthetic materials with well defined micro- and macro-sized features, but extending control to the nanoscale remains challenging5,6 . DNA nanotechnology can deliver a wide range of customized nanoscale two- and three-dimensional assemblies with controlled sizes and shapes7-11 ...
July 2018: Nature
https://www.readbyqxmd.com/read/29985341/biotin-based-pulldown-assay-to-validate-mrna-targets-of-cellular-mirnas
#5
Sabyasachi Dash, Muthukumar Balasubramaniam, Chandravanu Dash, Jui Pandhare
MicroRNAs (miRNAs) are a class of small noncoding RNAs that post-transcriptionally regulate cellular gene expression. MiRNAs bind to the 3' untranslated region (UTR) of target mRNA to inhibit protein translation or in some instances cause mRNA degradation. The binding of the miRNA to the 3' UTR of the target mRNA is mediated by a 2-8 nucleotide seed sequence at the 5' end of miRNA. While the role of miRNAs as cellular regulatory molecules is well established, identification of the target mRNAs with functional relevance remains a challenge...
June 12, 2018: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29984479/uniform-core-shell-nanoparticles-with-thiolated-hyaluronic-acid-coating-to-enhance-oral-delivery-of-insulin
#6
Houkuan Tian, Zhiyu He, Chengxin Sun, Chengbiao Yang, Pengfei Zhao, Lixin Liu, Kam W Leong, Hai-Quan Mao, Zhijia Liu, Yongming Chen
Oral delivery of protein drugs is an attractive route of administration due to its convenience for repeated dosing and good patient compliance. However, currently oral protein therapeutics show very low bioavailability mainly due to the existence of hostile gastrointestinal (GI) environments, including mucus layers and intestinal epithelial barriers. Herein, using insulin as a model protein therapeutic, the core-shell nanoparticles with thiolated hyaluronic acid (HA-SH) coating (NPHA-SH ) are produced utilizing a two-step flash nanocomplexation process to enhance oral delivery efficiency of insulin...
July 8, 2018: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/29983071/kinetically-stable-triglyceride-based-nanodroplets-and-their-interactions-with-lipid-specific-proteins
#7
Valerija Vezočnik, Vesna Hodnik, Simona Sitar, Halil I Okur, Magda Tušek-Žnidarič, Cornelis Lütgebaucks, Kristina Sepčić, Ksenija Kogej, Sylvie Roke, Ema Žagar, Peter Maček
Understanding of the interactions between proteins and natural and artificially prepared lipid membrane surfaces and embedded nonpolar cores is important in studies of physiological processes and their pathologies and is applicable to nanotechnologies. In particular, rapidly growing interest in cellular droplets defines the need for simplified biomimetic lipid model systems to overcome in vivo complexity and variability. We present a protocol for the preparation of kinetically stable nanoemulsions with nanodroplets composed of sphingomyelin (SM) and cholesterol (Chol), as amphiphilic surfactants, and trioleoylglycerol (TOG), at various molar ratios...
July 31, 2018: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/29982117/magnetic-force-assisted-electrochemical-sensor-for-the-detection-of-thrombin-with-aptamer-antibody-sandwich-formation
#8
Saeromi Chung, Jong-Min Moon, Jaekyu Choi, Hyundoo Hwang, Yoon-Bo Shim
A magnetic force assisted electrochemical aptamer-antibody sandwich assay (MESA) was developed for the detection of thrombin as a model protein in serum samples. The MESA using the formation of sandwich complexes on the electrochemical sensor probe for reaction and the removal of unbound bioconjugates from the sensor surface without washing are controlled by a magnetic field. Thrombin was determined by the cathodic currents of a toluidine blue O (TBO) attached with thrombin antibody modified magnetic nanoparticle (MNP) at the sensor surface...
July 5, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29962903/optimizing-crystal-size-of-photosystem-ii-by-macroseeding-toward-neutron-protein-crystallography
#9
Rana Hussein, Mohamed Ibrahim, Ruchira Chatterjee, Leighton Coates, Frank Müh, Vittal K Yachandra, Junko Yano, Jan Kern, Holger Dobbek, Athina Zouni
Photosystem II (PSII) catalyzes the photo-oxidation of water to molecular oxygen and protons. The water splitting reaction occurs inside the oxygen-evolving complex (OEC) via a Mn4 CaO5 cluster. To elucidate the reaction mechanism, detailed structural information for each intermediate state of the OEC is required. Despite the current high-resolution crystal structure of PSII at 1.85 Å and other efforts to follow the structural changes of the Mn4 CaO5 cluster using X-ray free electron laser (XFEL) crystallography in addition to spectroscopic methods, many details about the reaction mechanism and conformational changes in the catalytic site during water oxidation still remain elusive...
January 3, 2018: Crystal Growth & Design
https://www.readbyqxmd.com/read/29939162/the-copii-cargo-adapter-sec24c-is-essential-for-neuronal-homeostasis
#10
Bo Wang, Joung Hyuck Joo, Rebecca Mount, Brett J W Teubner, Alison Krenzer, Amber L Ward, Viraj P Ichhaporia, Elizabeth J Adams, Rami Khoriaty, Samuel T Peters, Shondra M Pruett-Miller, Stanislav S Zakharenko, David Ginsburg, Mondira Kundu
SEC24 family members are components of the coat protein complex II (COPII) machinery that interact directly with cargo or with other adapters to ensure proper sorting of secretory cargo into COPII vesicles. SEC24C is 1 of 4 mammalian SEC24 paralogs (SEC24A-D), which segregate into 2 subfamilies on the basis of sequence homology (SEC24A/SEC24B and SEC24C/SEC24D). Here, we demonstrate that postmitotic neurons, unlike professional secretory cells in other tissues, are exquisitely sensitive to loss of SEC24C. Conditional KO of Sec24c in neural progenitors during embryogenesis caused perinatal mortality and microcephaly, with activation of the unfolded protein response and apoptotic cell death of postmitotic neurons in the murine cerebral cortex...
June 25, 2018: Journal of Clinical Investigation
https://www.readbyqxmd.com/read/29935054/tempest-in-a-sugar-coated-lab-vial
#11
EDITORIAL
Duska Dragun, Aurélie Philippe
Angiotensin II type 1 receptor (AT1 R) is a classical G-protein-coupled-receptor (GPCR) displaying complex structure consisting of 7-transmembrane helices connected by intracellular and extracellular loops. Beside Angiotensin II binding within transmembrane sites and mechanically induced ligand free activation, AT1 R can be also activated by agonistic autoantibodies (AT1 R-Ab) recognizing conformational epitopes contained in the second extracellular loop. Direct pathophysiologic involvement of AT1 R-Abs is well established in several autoimmune contexts and organ transplantation (1)...
June 23, 2018: American Journal of Transplantation
https://www.readbyqxmd.com/read/29889083/effect-of-insulin-coated-trimethyl-chitosan-nanoparticles-on-igf-1-igf-2-and-apoptosis-in-the-hippocampus-of-diabetic-male-rats
#12
Giti Kalantarian, Nasrin Ziamajidi, Reza Mahjub, Mohammad Taghi Goodarzi, Massoud Saidijam, Sara Soleimani Asl, Roghayeh Abbasalipourkabir
BACKGROUND: Subcutaneous injection of insulin can lead to problems such as hypoglycemia and edema. OBJECTIVE: The purpose of this research was to evaluate the effect of oral insulin-coated trimethyl chitosan nanoparticles on control of glycemic status, IGF-1 and IGF-2 levels, and apoptosis in the hippocampus of rats with diabetes mellitus. METHODS: Insulin-coated trimethyl chitosan nanoparticles were prepared by the polyelectrolyte complex method (PEC) method...
June 6, 2018: Restorative Neurology and Neuroscience
https://www.readbyqxmd.com/read/29882778/a-wd40-repeat-protein-from-camellia-sinensis-regulates-anthocyanin-and-proanthocyanidin-accumulation-through-the-formation-of-myb%C3%A2-bhlh%C3%A2-wd40-ternary-complexes
#13
Yajun Liu, Hua Hou, Xiaolan Jiang, Peiqiang Wang, Xinlong Dai, Wei Chen, Liping Gao, Tao Xia
Flavan-3-ols and oligomeric proanthocyanidins (PAs) are the main nutritional polyphenols in green tea ( Camellia sinensis ), which provide numerous benefits to human health. To date, the regulatory mechanism of flavan-3-ol biosynthesis in green tea remains open to study. Herein, we report the characterization of a C. sinensis tryptophan-aspartic acid repeat protein (CsWD40) that interacts with myeloblastosis (MYB) and basic helix-loop-helix (bHLH) transcription factors (TFs) to regulate the biosynthesis of flavan-3-ols...
June 6, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29870684/the-binding-of-tbk1-to-sting-requires-exocytic-membrane-traffic-from-the-er
#14
Emari Ogawa, Kojiro Mukai, Kota Saito, Hiroyuki Arai, Tomohiko Taguchi
Stimulator of interferon genes (STING) is essential for the type I interferon and pro-inflammatory responses against DNA pathogens. In response to the presence of cytosolic DNA, STING translocates from the endoplasmic reticulum (ER) to the Golgi, and activates TANK-binding kinase 1 (TBK1), a cytosolic kinase that is essential for the activation of STING-dependent downstream signalling. The organelles where TBK1 binds to STING remain unknown. Here we show that TBK1 binds to STING at the Golgi, not at the ER...
June 7, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29806182/mitogen-activated-protein-kinase-phosphatase-1-reduces-the-replication-efficiency-of-bamboo-mosaic-virus-in-nicotiana-benthamiana
#15
Cheng-Cheng Lee, Yi-Jhen Wu, Chia-Hsin Hsueh, Yu-Ting Huang, Yau-Heiu Hsu, Menghsiao Meng
In plants, the mitogen-activated protein kinase (MAPK) cascades are the central signaling pathways of the complicated defense network triggered by the perception of pathogen-associated molecular patterns to repel pathogens. The Arabidopsis thaliana MAPK phosphatase 1 (AtMKP1) negatively regulates the activation of MAPKs. Recently, the AtMKP1 homolog of Nicotiana benthamiana (NbMKP1) was found in association with the Bamboo mosaic virus (BaMV) replication complex. This study aimed to investigate the role of NbMKP1 in BaMV multiplication in N...
May 28, 2018: Molecular Plant Pathology
https://www.readbyqxmd.com/read/29793951/varicella-zoster-virus-orf9p-binding-to-cellular-adaptor-protein-complex-1-is-important-for-viral-infectivity
#16
Marielle Lebrun, Julien Lambert, Laura Riva, Nicolas Thelen, Xavier Rambout, Caroline Blondeau, Marc Thiry, Robert Snoeck, Jean-Claude Twizere, Franck Dequiedt, Graciela Andrei, Catherine Sadzot-Delvaux
ORF9p (homologous to herpes simplex virus 1 [HSV-1] VP22) is a varicella-zoster virus (VZV) tegument protein essential for viral replication. Even though its precise functions are far from being fully described, a role in the secondary envelopment of the virus has long been suggested. We performed a yeast two-hybrid screen to identify cellular proteins interacting with ORF9p that might be important for this function. We found 31 ORF9p interaction partners, among which was AP1M1, the μ subunit of the adaptor protein complex 1 (AP-1)...
August 1, 2018: Journal of Virology
https://www.readbyqxmd.com/read/29780325/proteomics-of-stored-red-blood-cell-membrane-and-storage-induced-microvesicles-reveals-the-association-of-flotillin-2-with-band-3-complexes
#17
Michel Prudent, Julien Delobel, Aurélie Hübner, Corinne Benay, Niels Lion, Jean-Daniel Tissot
The storage of erythrocyte concentrates (ECs) induces lesions that notably affect metabolism, protein activity, deformability of red blood cells (RBCs), as well as the release of oxygen. Band 3 is one of the proteins affected during the ex vivo aging of RBCs. This membrane protein is an anion transporter, an anchor site for the cytoskeleton and other membrane proteins as well as a binding site for glycolytic enzymes and bears blood group antigens. In the present study, band 3 complexes were isolated from RBCs stored for 7 and 42 days in average ( n = 3), as well as from microvesicles ( n = 3)...
2018: Frontiers in Physiology
https://www.readbyqxmd.com/read/29759250/metal-chelation-dual-template-epitope-imprinting-polymer-via-distillation-precipitation-polymerization-for-recognition-of-porcine-serum-albumin
#18
Ya-Ping Qin, Hai-Yan Wang, Xi-Wen He, Wen-You Li, Yu-Kui Zhang
A novel dual-template epitope imprinting polymer coated on magnetic carbon nanotubes (MCNTs@D-EMIP) was successfully prepared for specific recognition of porcine serum albumin (PSA) via dual-template epitope imprinting, metal chelation imprinting and distillation-precipitation polymerization (DPP). C-terminal peptides and N-terminal peptides of PSA were selected as templates simultaneously, and zinc acrylate and ethylene glycol dimethacrylate (EGDMA) were used as functional monomer and cross-linker, respectively...
August 1, 2018: Talanta
https://www.readbyqxmd.com/read/29714092/cotton-leaf-curl-disease-which-whitefly-is-the-vector
#19
Li-Long Pan, Xi-Yun Cui, Qun-Fang Chen, Xiao-Wei Wang, Shu-Sheng Liu
Cotton leaf curl disease is one of the most significant constraints to the production of cotton. In the past decades our understanding of the begomoviruses (family Geminiviridae) causing the disease has improved, but little is known regarding transmission of these viruses by the different species of whiteflies in the Bemisia tabaci complex. We compared transmission efficiency of cotton leaf curl Multan virus (CLCuMuV), one of the major begomoviruses associated with cotton leaf curl disease, by four whitefly species, of which two are indigenous to Asia and two are invasive worldwide...
May 1, 2018: Phytopathology
https://www.readbyqxmd.com/read/29697006/toxicity-of-zno-nanoparticles-nps-with-or-without-hydrophobic-surface-coating-to-thp-1-macrophages-interactions-with-bsa-or-oleate-bsa
#20
Xianqiang Li, Xin Fang, Yanhuai Ding, Juan Li, Yi Cao
It is recently shown that biological macromolecules in food could interact with nanoparticles (NPs) and consequently change the biological effects of NPs. In this study, the interactions between ZnO NPs with or without hydrophobic surface coating and bovine serum albumin (BSA) or oleate (OA) complexed to BSA (OA-BSA) were assessed. Atomic force microscope (AFM) showed topographic changes of both types of NPs by BSA or OA-BSA, which could indicate the formation of protein corona. ZnO NPs showed negative Zeta potential, which was slightly decreased by BSA or OA-BSA, with OA-BSA being more effective...
September 2018: Toxicology Mechanisms and Methods
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