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Coat Protein Complex 1

Abigail Betanzos, Dxinegueela Zanatta, Cecilia Bañuelos, Elizabeth Hernández-Nava, Patricia Cuellar, Esther Orozco
In Entamoeba histolytica , the EhADH adhesin together with the EhCP112 cysteine protease, form a 124 kDa complex named EhCPADH. This complex participates in trophozoite adherence, phagocytosis and cytolysis of target cells. EhCPADH and EhCP112 are both involved on epithelium damage, by opening tight junctions (TJ) and reaching other intercellular junctions. EhADH is a scaffold protein belonging to the ALIX family that contains a Bro1 domain, expresses at plasma membrane, endosomes and cytoplasm of trophozoites, and is also secreted to the medium...
2018: Frontiers in Cellular and Infection Microbiology
Ana M L Sousa, Tai-De Li, Sabu Varghese, Peter James Halling, King Hang Aaron Lau
Proteins represent complex biomolecules capable of wide-ranging but also highly specific functionalities. Their immobilization on material supports can enable broad applications from sensing, industrial biocatalysis, to biomedical interfaces and materials. We demonstrate the advantages of using aqueous-processed crosslinked polyphenol coatings for immobilizing proteins, including IgG, avidin, and various single and multi-domain enzymes on diverse materials, to enable active biofunctional structures (e.g. ca...
October 9, 2018: ACS Applied Materials & Interfaces
Wataru Yamazaki, Ryohei Makino, Konomu Nagao, Hirohisa Mekata, Kenji Tsukamoto
Transboundary animal diseases, including highly pathogenic avian influenza, cause vast economic losses throughout the world. While it is important to identify the sources and propagation routes of the spread, such strategies are often hindered by incomplete epidemiological evidence. Isolation/detection of micro-amounts of pathogens from environmental samples is rarely successful due to the very low contamination level. This paper describes the development of the micro-amount of virion enrichment technique (MiVET), a simple and highly sensitive method that combines the use of a complex comprising a polyclonal antibody and protein G-coated magnetic beads for virion capture, and simple sodium dodecyl benzenesulfonate (SDBS) elution for low volume samples...
September 29, 2018: Transboundary and Emerging Diseases
Hanzey Yasar, Alexander Biehl, Chiara De Rossi, Marcus Koch, Xabi Murgia, Brigitta Loretz, Claus-Michael Lehr
BACKGROUND: Messenger RNA (mRNA) has gained remarkable attention as an alternative to DNA-based therapies in biomedical research. A variety of biodegradable nanoparticles (NPs) has been developed including lipid-based and polymer-based systems for mRNA delivery. However, both systems still lack in achieving an efficient transfection rate and a detailed understanding of the mRNA transgene expression kinetics. Therefore, quantitative analysis of the time-dependent translation behavior would provide a better understanding of mRNA's transient nature and further aid the enhancement of appropriate carriers with the perspective to generate future precision nanomedicines with quick response to treat various diseases...
September 19, 2018: Journal of Nanobiotechnology
Yuefei Shen, Jessica L Cohen, Sarah M Nicoloro, Mark Kelly, Batuhan Yenilmez, Felipe Henriques, Emmanouela Tsagkaraki, Yvonne J K Edwards, Xiaodi Hu, Randall H Friedline, Jason K Kim, Michael P Czech
RNA-guided, engineered nucleases derived from the prokaryotic adaptive immune system CRISPR-Cas represent a powerful platform for gene deletion and editing. When used as a therapeutic approach, direct delivery of Cas9 protein and single-guide (sg)RNA could circumvent the safety issues associated with plasmid delivery and therefore represents an attractive tool for precision genome engineering. Gene deletion or editing in adipose tissue to enhance its energy expenditure, fatty acid oxidation, and secretion of bioactive factors through a "browning" process presents a potential therapeutic strategy to alleviate metabolic disease...
September 6, 2018: Journal of Biological Chemistry
Thiago Coimbra Pimenta, Cátia da Cruz Santos, Ronaldo Luís Thomasini, Lucas Franco Ferreira
Dengue is an infectious viral disease transmitted by the Aedes aegypti mosquito, the control of which is complex. In addition, the clinical diagnosis is difficult to perform since it resembles other febrile infections; thus, the development of more effective methods to detect dengue virus (DV) has drawn increasing attention. The present study aimed to develop an impedimetric immunosensor for dengue diagnosis using a screen-printed electrode (SPE) functionalized with polymer films derived from 4-aminophenylacetic acid (4-APA)...
September 4, 2018: Biomedical Microdevices
Andrés Trávez, Yoana Rabanal-Ruiz, Jaime López-Alcalá, Laura Molero-Murillo, Alberto Díaz-Ruiz, Rocío Guzmán-Ruiz, Victoria Catalán, Amaia Rodríguez, Gema Frühbeck, Francisco J Tinahones, Stéphane Gasman, Nicolas Vitale, Yolanda Jiménez-Gómez, María M Malagón
Adipocyte dysfunction in obesity is commonly associated with impaired insulin signalling in adipocytes and insulin resistance. Insulin signalling has been associated with caveolae, which are coated by large complexes of caveolin and cavin proteins, along with proteins with membrane-binding and remodelling properties. Here, we analysed the regulation and function of a component of caveolae involved in growth factor signalling in neuroendocrine cells, neuroendocrine long coiled-coil protein-2 (NECC2), in adipocytes...
August 30, 2018: Journal of Cellular and Molecular Medicine
Delfina P González, Helen V Lamb, Diana Partida, Zachary T Wilson, Marie-Claire Harrison, Julián A Prieto, James J Moresco, Jolene K Diedrich, John R Yates, Sara K Olson
Metazoan eggs have a specialized coat of extracellular matrix that aids in sperm-egg recognition. The coat is rapidly remodeled after fertilization to prevent polyspermy and establish a more permanent barrier to protect the developing embryo. In nematodes, this coat is called the vitelline layer, which is remodeled into the outermost layer of a rigid and impermeable eggshell. We have identified three key components of the vitelline layer structural scaffold - PERM-2, PERM-4 and CBD-1, the first such proteins to be described in the nematode C...
October 15, 2018: Developmental Biology
Takeshi Yamasaki, Akio Suzuki, Rie Hasebe, Motohiro Horiuchi
Intracellular dynamics of an abnormal isoform of prion protein (PrPSc ) are tightly associated with prion propagation. However, the machineries involved in the intracellular trafficking of PrPSc are not fully understood. Our previous study suggested that PrPSc in persistently prion-infected cells dynamically circulates between endocytic-recycling compartments (ERCs) and peripheral regions of the cells. To investigate these machineries, we focused on retrograde transport from endosomes to the trans-Golgi network, which is one of the pathways involved in recycling of molecules...
August 16, 2018: Scientific Reports
Tomoo Shimada, Tadashi Kunieda, Sakura Sumi, Yasuko Koumoto, Kentaro Tamura, Kyoko Hatano, Haruko Ueda, Ikuko Hara-Nishimura
The adaptor protein (AP) complexes play crucial roles in vesicle formation in post-Golgi trafficking. Land plants have five types of AP complexes (AP-1 to AP-5), each of which consists of two large subunits, one medium subunit, and one small subunit. Here, we show that Arabidopsis AP-1 complex mediates the polarized secretion and accumulation of a pectic polysaccharide called mucilage in seed coat cells. Previously, a loss-of-function mutant of AP1M2, the medium subunit of AP-1, has been shown to display deleterious growth defects because of defective cytokinesis...
August 7, 2018: Plant & Cell Physiology
Linglin Fu, Jinru Zhou, Chong Wang, Xiaohui Li, Lei Zheng, Yanbo Wang
Tropomyosin (TM) and arginine kinase (AK) are known as two major allergens in seafood. For the first time, we demonstrate a newly developed ion-exchange chromatography coupled with dynamic coating capillary electrophoresis (IEC-DCCE) method to simultaneously analyze the TM and AK in shellfish. First, we have optimized the procedure of IEC for simple enrichment of TM and AK crude extract. By using 30 mM borate-borax at pH 9.0 with 0.3% (v/v) Tween-20 as a dynamic coating modifier for capillary electrophoresis (CE) separation, the migration time, separation efficiency and electrophoretic resolution greatly improved...
2018: Frontiers in Chemistry
Erika Sabella, Roberto Pierro, Andrea Luvisi, Alessandra Panattoni, Claudio D'Onofrio, Giancarlo Scalabrelli, Eliana Nutricati, Alessio Aprile, Luigi De Bellis, Alberto Materazzi
The health status of the native grapevine Vitis vinifera subsp. sylvestris (Gmeli) Hegi in natural areas in Europe has received little attention. A survey was carried out on wild grapevines in Tuscany (Italy), where isolates of the Grapevine rupestris stem pitting virus (GRSPaV), Grapevine leafroll-associated virus 1 and 3 (GLRaV-1 and GLRaV-3) and Grapevine virus A (GVA) were detected. The complete coat protein (CP) region of these isolates was sequenced to investigate the relationship of the viral variants from Tuscan wild grapevines with isolates from different geographical origins...
2018: PloS One
Sonia Sánchez-Campos, Guillermo Domínguez-Huerta, Luis Díaz-Martínez, Diego M Tomás, Jesús Navas-Castillo, Enrique Moriones, Ana Grande-Pérez
Geminiviruses (family Geminiviridae ) possess single-stranded circular DNA genomes that are replicated by cellular polymerases in plant host cell nuclei. In their hosts, geminivirus populations behave as ensembles of mutant and recombinant genomes, known as viral quasispecies. This favors the emergence of new geminiviruses with altered host range, facilitating new or more severe diseases or overcoming resistance traits. In warm and temperate areas several whitefly-transmitted geminiviruses of the genus Begomovirus cause the tomato yellow leaf curl disease (TYLCD) with significant economic consequences...
2018: Frontiers in Plant Science
Xiaoguo Liu, Fei Zhang, Xinxin Jing, Muchen Pan, Pi Liu, Wei Li, Bowen Zhu, Jiang Li, Hong Chen, Lihua Wang, Jianping Lin, Yan Liu, Dongyuan Zhao, Hao Yan, Chunhai Fan
Genetically encoded protein scaffolds often serve as templates for the mineralization of biocomposite materials with complex yet highly controlled structural features that span from nanometres to the macroscopic scale1-4 . Methods developed to mimic these fabrication capabilities can produce synthetic materials with well defined micro- and macro-sized features, but extending control to the nanoscale remains challenging5,6 . DNA nanotechnology can deliver a wide range of customized nanoscale two- and three-dimensional assemblies with controlled sizes and shapes7-11 ...
July 2018: Nature
Sabyasachi Dash, Muthukumar Balasubramaniam, Chandravanu Dash, Jui Pandhare
MicroRNAs (miRNAs) are a class of small noncoding RNAs that post-transcriptionally regulate cellular gene expression. MiRNAs bind to the 3' untranslated region (UTR) of target mRNA to inhibit protein translation or in some instances cause mRNA degradation. The binding of the miRNA to the 3' UTR of the target mRNA is mediated by a 2-8 nucleotide seed sequence at the 5' end of miRNA. While the role of miRNAs as cellular regulatory molecules is well established, identification of the target mRNAs with functional relevance remains a challenge...
June 12, 2018: Journal of Visualized Experiments: JoVE
Houkuan Tian, Zhiyu He, Chengxin Sun, Chengbiao Yang, Pengfei Zhao, Lixin Liu, Kam W Leong, Hai-Quan Mao, Zhijia Liu, Yongming Chen
Oral delivery of protein drugs is an attractive route of administration due to its convenience for repeated dosing and good patient compliance. However, currently oral protein therapeutics show very low bioavailability mainly due to the existence of hostile gastrointestinal (GI) environments, including mucus layers and intestinal epithelial barriers. Herein, using insulin as a model protein therapeutic, the core-shell nanoparticles with thiolated hyaluronic acid (HA-SH) coating (NPHA-SH ) are produced utilizing a two-step flash nanocomplexation process to enhance oral delivery efficiency of insulin...
September 2018: Advanced Healthcare Materials
Valerija Vezočnik, Vesna Hodnik, Simona Sitar, Halil I Okur, Magda Tušek-Žnidarič, Cornelis Lütgebaucks, Kristina Sepčić, Ksenija Kogej, Sylvie Roke, Ema Žagar, Peter Maček
Understanding of the interactions between proteins and natural and artificially prepared lipid membrane surfaces and embedded nonpolar cores is important in studies of physiological processes and their pathologies and is applicable to nanotechnologies. In particular, rapidly growing interest in cellular droplets defines the need for simplified biomimetic lipid model systems to overcome in vivo complexity and variability. We present a protocol for the preparation of kinetically stable nanoemulsions with nanodroplets composed of sphingomyelin (SM) and cholesterol (Chol), as amphiphilic surfactants, and trioleoylglycerol (TOG), at various molar ratios...
July 31, 2018: Langmuir: the ACS Journal of Surfaces and Colloids
Saeromi Chung, Jong-Min Moon, Jaekyu Choi, Hyundoo Hwang, Yoon-Bo Shim
A magnetic force assisted electrochemical aptamer-antibody sandwich assay (MESA) was developed for the detection of thrombin as a model protein in serum samples. The MESA using the formation of sandwich complexes on the electrochemical sensor probe for reaction and the removal of unbound bioconjugates from the sensor surface without washing are controlled by a magnetic field. Thrombin was determined by the cathodic currents of a toluidine blue O (TBO) attached with thrombin antibody modified magnetic nanoparticle (MNP) at the sensor surface...
October 15, 2018: Biosensors & Bioelectronics
Rana Hussein, Mohamed Ibrahim, Ruchira Chatterjee, Leighton Coates, Frank Müh, Vittal K Yachandra, Junko Yano, Jan Kern, Holger Dobbek, Athina Zouni
Photosystem II (PSII) catalyzes the photo-oxidation of water to molecular oxygen and protons. The water splitting reaction occurs inside the oxygen-evolving complex (OEC) via a Mn4 CaO5 cluster. To elucidate the reaction mechanism, detailed structural information for each intermediate state of the OEC is required. Despite the current high-resolution crystal structure of PSII at 1.85 Å and other efforts to follow the structural changes of the Mn4 CaO5 cluster using X-ray free electron laser (XFEL) crystallography in addition to spectroscopic methods, many details about the reaction mechanism and conformational changes in the catalytic site during water oxidation still remain elusive...
January 3, 2018: Crystal Growth & Design
Bo Wang, Joung Hyuck Joo, Rebecca Mount, Brett J W Teubner, Alison Krenzer, Amber L Ward, Viraj P Ichhaporia, Elizabeth J Adams, Rami Khoriaty, Samuel T Peters, Shondra M Pruett-Miller, Stanislav S Zakharenko, David Ginsburg, Mondira Kundu
SEC24 family members are components of the coat protein complex II (COPII) machinery that interact directly with cargo or with other adapters to ensure proper sorting of secretory cargo into COPII vesicles. SEC24C is 1 of 4 mammalian SEC24 paralogs (SEC24A-D), which segregate into 2 subfamilies on the basis of sequence homology (SEC24A/SEC24B and SEC24C/SEC24D). Here, we demonstrate that postmitotic neurons, unlike professional secretory cells in other tissues, are exquisitely sensitive to loss of SEC24C. Conditional KO of Sec24c in neural progenitors during embryogenesis caused perinatal mortality and microcephaly, with activation of the unfolded protein response and apoptotic cell death of postmitotic neurons in the murine cerebral cortex...
August 1, 2018: Journal of Clinical Investigation
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