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antiviral method

Claudia Palladino, Ifeanyi Jude Ezeonwumelu, Rute Marcelino, Verónica Briz, Inês Moranguinho, Fátima Serejo, José Fernando Velosa, Rui Tato Marinho, Pedro Borrego, Nuno Taveira
Any successful strategy to prevent and control HCV infection requires an understanding of the epidemic behaviour among the different genotypes. Here, we performed the first characterization of the epidemic history and transmission dynamics of HCV subtypes in Portugal. Direct sequencing of NS5B was performed on 230 direct-acting antiviral drugs (DAA)-treatment naïve patients in Lisbon. Phylogenetic analysis was used for subtyping and transmission cluster identification. Bayesian methods were used to reconstruct the epidemic history of HCV subtypes...
August 16, 2018: Scientific Reports
Daniela Iannazzo, Alessandro Pistone, Stefania Ferro, Laura De Luca, Annamaria Monforte, Roberto Romeo, Maria Rosa Buemi, Christophe Pannecouque
Graphene quantum dots (GQD) are the next generation of nanomaterials with great potential in drug delivery and target-specific HIV inhibition. In this study we investigated the antiviral activity of graphene based nanomaterials by using water soluble GQD synthesized from multi-walled carbon nanotubes (MWCNT) through prolonged acidic oxidation and exfoliation and compared their anti-HIV activity with that exerted by reverse transcriptase inhibitors (RTI) conjugated with the same nanomaterial. The antiretroviral agents chosen in this study, CHI499 and CDF119, belong to the class of non-nucleoside reverse transcriptase inhibitors (NNRTI)...
August 14, 2018: Bioconjugate Chemistry
Huiying Zhao, Ghazaleh Taherzadeh, Yaoqi Zhou, Yuedong Yang
Protein-carbohydrate interaction is essential for biological systems, and carbohydrate-binding proteins (CBPs) are important targets when designing antiviral and anticancer drugs. Due to the high cost and difficulty associated with experimental approaches, many computational methods have been developed as complementary approaches to predict CBPs or carbohydrate-binding sites. However, most of these computational methods are not publicly available. Here, we provide a comprehensive review of related studies and demonstrate our two recently developed bioinformatics methods...
August 14, 2018: Current Protocols in Protein Science
Leng Wei Khoo, Siew Audrey Kow, Ming Tatt Lee, Chin Ping Tan, Khozirah Shaari, Chau Ling Tham, Faridah Abas
Clinacanthus nutans (Burm.f.) Lindau (Acanthaceae), commonly known as Sabah snake grass, is a vegetable and a well-known herb that is considered an alternative medicine for insect bites, skin rashes, herpes infection, inflammation, and cancer and for health benefits. Current review aims to provide a well-tabulated repository of the phytochemical screening, identification and quantification, and the pharmacological information of C. nutans according to the experimental design and the plant preparation methods which make it outstanding compared to existing reviews...
2018: Evidence-based Complementary and Alternative Medicine: ECAM
Joanna Joyce, J Embree, H Pymar, V Poliquin
BACKGROUND: Neonatal herpes simplex virus (HSV) infection and its implications have been well defined. Several methods are recommended to mitigate the risk of maternal transmission of HSV to the neonate, including CS, suppressive antiviral therapy for the mother, and prophylaxis for the infant. The utility of CS in women who present with a duration of rupture of membranes greater than 4 hours remains a question. CASE: We present a case of a woman who presented following 10 hours of rupture of membranes with HSV genital lesions, suspected to be the result of untreated recurrent infection...
August 2018: Journal of Obstetrics and Gynaecology Canada: JOGC, Journal D'obstétrique et Gynécologie du Canada: JOGC
Robert L Atmar, Sasirekha Ramani, Mary K Estes
PURPOSE OF REVIEW: Noroviruses are a major cause of gastroenteritis. This review summarizes new information on noroviruses that may lead to the development of improved measures for limiting their human health impact. RECENT FINDINGS: GII.4 strains remain the most common human noroviruses causing disease, although GII.2 and GII.17 strains have recently emerged as dominant strains in some populations. Histo-blood group antigen (HBGA) expression on the gut mucosa drives susceptibility to different norovirus strains...
August 9, 2018: Current Opinion in Infectious Diseases
Alina M Yelins'ka, Olena O Shvaykovs'ka, Vitalii O Kostenko
OBJECTIVE: Introduction: The connective tissue remodeling is essential for periodontal and salivary glands (SG) pathology. Recently there has been demonstrated the number of pharmacological effects of green tea (Camellia sinensis) such as antioxidant, anti-inflammatory, anti-aging, antibacterial, antiviral and DNA-protective effects, associated with the presence of epigallocatechin-3-gallate (EGCG) as an inducer of the Keap1 / Nrf2 / antioxidant response element signaling pathway. However, the EGCG effects on the components of soft connective tissues of periodontium and SG are still unclear...
2018: Wiadomości Lekarskie: Organ Polskiego Towarzystwa Lekarskiego
Deping Yang, Tingting Hu, Xuan Wu, Ke Li, Qi Zhong, Weiwei Liu
This study aimed to establish a quantitative droplet digital polymerase chain reaction (ddPCR) detection system for clinical hepatitis B virus (HBV) DNA in samples from patients with HBV infection with the aim of monitoring fluctuations and planning future antiviral treatments. Primers and probes for the conserved region of HBV DNA sequence (GenBank: AB554017.1) were designed by sequence alignment with the HBV DNA sequence in GenBank. The PUC57 plasmid containing the gene fragment was then synthesized according to the fragment amplified by primer...
August 7, 2018: Journal of Medical Virology
Anoop Kumar, Manoj Kumar Rajput, Deepika Paliwal, Aakanksha Yadav, Reba Chhabra, Surinder Singh
Hepatitis C virus (HCV) infection is a blood borne and transfusion-transmitted infection (TTI). It has emerged as one of the major health challenges worldwide. In India, around 12-18 million peoples are infected with HCV, but in terms of prevalence percentage, its looks moderate due to large population. The burden of the HCV infection increases due to lack of foolproof screening of blood and blood products before transfusion. The qualified screening and quantification of HCV play an important role in diagnosis and treatment of HCV-related diseases...
May 2018: Indian Journal of Medical Research
Mohamed A Abdel-Lateef, Mahmoud A Omar, Ramadan Ali, Sayed M Derayea
Daclatasvir dihydrochloride (DAC) is a new, direct-acting antiviral drug with powerful inhibitory effect against all hepatitis C virus (HCV) genotypes. A sensitive, simple, fast and specific fluorometric method for estimation of DAC in the presence of sofosbuvir was developed and validated. The method is based on reinforcement the fluorescence intensity of DAC by 170% of its original value in an aqueous solution of hexadecyl trimethyl ammonium bromide (pH 5.5, Teorell and Stenhagen buffer). The fluorescence intensity measurements were accomplished at 387 nm with 328 nm for excitation wavelength...
August 1, 2018: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
Yang Zhao, Dong Liu, Peter Proksch, Demin Zhou, Wenhan Lin
Chemical examination of the EtOAc extract of the sponge-associated fungus Truncatella angustata in solid culture led to the isolation of eight undescribed isoprenylated cyclohexanols, namely truncateols O-V together with 14 known analogues. Their structures were determined on the basis of extensive spectroscopic analyses, including the modified Mosher's method and ECD data for the assignment of their absolute configurations. Truncateol O exhibited significant inhibition toward both HIV-1 and H1 N1 virus, while truncateol P exerted inhibitory effect against HIV-1 virus...
August 1, 2018: Phytochemistry
David C Brice, Zsolt Toth, Gill Diamond
Oral epithelial cells (OECs) represent the first line of defense against viruses that are spread via saliva, including Kaposi's sarcoma-associated herpesvirus (KSHV). Infection of humans by KSHV and viral pathogenesis begins by infecting OECs. One method OECs use to limit viral infections in the oral cavity is the production of antimicrobial peptides (AMPs), or host defense peptides (HDPs). However, no studies have investigated the antiviral activities of any HDP against KSHV. The goal of this study was to determine the antiviral activity of one HDP, LL-37, against KSHV in the context of infecting OECs...
August 1, 2018: Antiviral Research
Xiaoli Han, Jinhua He, Qinyue Chen, Yali Sun, Xiulei Zhang, Zhengyi Gu, Xianyi Sha
Artemitin, a significant flavonol compound existing in Laggera pterodonta (DC.) Benth., Artemisia rupestris L, etc, is pyramidally focused by researchers owing to its pharmacological activities (such as antioxidative, anti-inflammatory, and antiviral). In this work, a highly sensitive and specific high-performance liquid chromatography -electrospray ionization-tandem mass spectrometry (HPLC-ESI-MS/MS) assay combined with protein precipitation has been established and validated for determining Artemitin concentration in rat plasma...
August 2, 2018: Biomedical Chromatography: BMC
Zheng Li, Zhengqing Lei, Yong Xia, Jun Li, Kui Wang, Han Zhang, Xuying Wan, Tian Yang, Weiping Zhou, Mengchao Wu, Timothy M Pawlik, Wan Yee Lau, Feng Shen
Importance: A reduced incidence of microvascular invasion (MVI) in hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC) may be associated with a decreased risk of early tumor recurrence and better survival after partial hepatectomy. Objective: To examine the association of preoperative antiviral treatment (AVT) with the incidences of MVI and posthepatectomy early tumor recurrence in HBV-related HCC. Design, Setting, and Participants: Data on a cohort of 2362 patients who underwent R0 resection for HBV-related HCC between January 2008 and April 2010 at the Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai, China, were reviewed...
August 1, 2018: JAMA Surgery
Mariana Bernadett Arregui, Gregorio Juan Mc Callum, Ignacio Smith, Marcela Solange Villaverde, Federico Javier Wolman, Alexandra Marisa Targovnik, María Victoria Miranda
Feline interferon beta is a cytokine that belongs to the type I IFN family, with antitumor, antiviral and immunomodulatory functions. In this work, recombinant feline interferon beta (rFeIFNβ) was expressed in insect larvae that constitute important agronomic plagues. rFeIFNβ accumulated in the hemolymph of Spodoptera frugiperda larvae infected with recombinant baculovirus and was purified by Blue-Sepharose chromatography directly from larval homogenates on day 4 post-infection. rFeIFNβ was recovered after purification with a specific activity of 1 × 106  IU mg-1 ...
August 2018: 3 Biotech
Katarzyna Turecka, Agnieszka Chylewska, Anna Kawiak, Krzysztof F Waleron
Although many antifungal agents are available in clinical treatment, increasing resistance of fungi, especially Candida species, to the available drugs requires the development of new safe and non-toxic compounds with novel modes of action as effective treatment against resistant microorganisms. Cobalt complexes are very interesting and attractive as potential candidates with antimicrobial activity. Their therapeutic uses as antiviral, antibacterial antifungal, antiparasitic, antitumour, transferrin transporters, and anti-inflammatory agents are being intensively investigated...
2018: Frontiers in Microbiology
Antonio Rosato, Alessia Carocci, Alessia Catalano, Maria Lisa Clodoveo, Carlo Franchini, Filomena Corbo, Giuseppe Gerardo Carbonara, Antonio Carrieri, Giuseppe Fracchiolla
Mentha piperita L. essential oil (EO) is employed for external use as antipruritic, astringent, rubefacient and antiseptic. Several studies demonstrated its significant antiviral, antifungal and antibacterial properties. The aim of this work is the study of the synergistic effects of M. piperita EO with antibacterials and antifungals that are widely available and currently prescribed in therapies against infections. The observed strong synergy may constitute a potential new approach to counter the increasing phenomenon of multidrug resistant bacteria and fungi...
2018: PloS One
Wilson R Tavares, Ana M L Seca
Background: Plants and their derived natural compounds possess various biological and therapeutic properties, which turns them into an increasing topic of interest and research. Juniperus genus is diverse in species, with several traditional medicines reported, and rich in natural compounds with potential for development of new drugs. Methods: The research for this review were based in the Scopus and Web of Science databases using terms combining Juniperus , secondary metabolites names, and biological activities...
July 31, 2018: Medicines (Basel, Switzerland)
Bastian Neesgaard, Morten Ruhwald, Henrik B Krarup, Nina Weis
BACKGROUND: With the introduction of direct acting antivirals, treatment of hepatitis C virus (HCV) in hard-to-reach populations is now feasible. Therefore, new cost-effective and reliable test methods are needed. Determination of HCV antibodies and HCV-RNA from dried blood spots samples could represent one such method. Here we examined whether anti-HCV could be detected-and HCV-RNA quantified-from dried blood spots, sent by regular mail. We also investigated, if IP-10 determined from dried blood spots correlated with fibrosis progression appraised by transient elastography...
2018: PloS One
Jean A Bernatchez, Zunhua Yang, Michael Coste, Jerry Li, Sungjun Beck, Yan Liu, Alex E Clark, Zhe Zhu, Lucas A Luna, Christal D Sohl, Byron W Purse, Rongshi Li, Jair L Siqueira-Neto
Zika virus (ZIKV) has been linked to the development of microcephaly in newborns, as well as Guillain-Barré syndrome. There are currently no drugs available to treat infection, and accordingly there is an unmet medical need for discovery of new therapies. High-throughput drug screening efforts focusing on indirect readouts of cell viability are prone to a higher frequency of false positives in cases where the virus is viable in the cell but the cytopathic effect is reduced or delayed. Here, we describe a fast and label-free phenotypic high-content imaging assay used to detect cells affected by the viral-induced cytopathic effect (CPE) using automated imaging and analysis...
July 30, 2018: Antimicrobial Agents and Chemotherapy
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