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https://www.readbyqxmd.com/read/30552495/a-novel-switchable-fluorescent-sensor-for-facile-and-highly-sensitive-detection-of-alkaline-phosphatase-activity-in-a-water-environment-with-gold-silver-nanoclusters
#1
Xiaoyan Wang, Zhenjiang Liu, Wanying Zhao, Jianfan Sun, Bin Qian, Xinwei Wang, Huawei Zeng, Daolin Du, Jinsheng Duan
A novel fluorescent sensor based on bovine serum albumin stabilized gold/silver nanoclusters (BSA-Au/Ag NCs) was developed for sensitive and facile detection of alkaline phosphatase (ALP) activity. For this fluorescent sensor, ascorbic acid 2-phosphate (AAP) was decomposed into ascorbic acid (AA) and phosphate by catalysis with ALP. The initial red fluorescence of the BSA-Au/Ag NCs was effectively quenched by KMnO4 and then the fluorescence was recovered by addition of AA. The mechanism of interaction between BSA-Au/Ag NCs and KMnO4 and AA was studied with use of the fluorescence lifetime and UV-vis absorption spectra...
December 15, 2018: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/30549037/wnt-signaling-reprograms-metabolism-in-dental-pulp-stem-cells
#2
Véronica Uribe-Etxebarria, Alice Agliano, Fernando Unda, Gaskon Ibarretxe
Human dental pulp stem cells (DPSCs) can differentiate to a wide range of different cell lineages, and share some gene expression and functional similarities with pluripotent stem cells. The stemness of DPSCs can also be pharmacologically enhanced by the activation of canonical Wnt signaling. Here, we examined the metabolic profile of DPSCs during reprogramming linked to Wnt activation, by a short (48 hr) exposure to either the GSK3-β inhibitor BIO (6-bromoindirubin-3´-oxine) or human recombinant protein WNT-3A...
December 13, 2018: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/30547850/phylogenetic-analysis-of-spirocerca-lupi-and-spirocerca-vulpis-reveal-high-genetic-diversity-and-intra-individual-variation
#3
Alicia Rojas, Eran Dvir, Róbert Farkas, Kalyan Sarma, Sonjoy Borthakur, Abdul Jabbar, Alex Markovics, Domenico Otranto, Gad Baneth
BACKGROUND: Spirocerca lupi is a parasitic nematode of canids that can lead to a severe and potentially fatal disease. Recently, a new species, Spirocerca vulpis, was described from red foxes in Europe, suggesting a high genetic diversity of the Spirocerca spp. infecting canids. The genetic variation and phylogenetic relationships of S. lupi collected from naturally-infected domestic dogs from Australia, Hungary, Israel, Italy, India and South Africa, and S. vulpis from red foxes from Bosnia and Herzegovina, Italy and Spain, was studied using mitochondrial and rDNA markers...
December 14, 2018: Parasites & Vectors
https://www.readbyqxmd.com/read/30544509/vo%C3%A2-b-graphene-composite-based-symmetrical-supercapacitor-electrode-via-screen-printing-for-intelligent-packaging
#4
Jieyu Zhang, Liangzhe Chen, Yixiang Wang, Shaoyong Cai, Yaqianzi Peng, Huijun Yang, Hao Yu, Fuyuan Ding, Chi Huang, Xinghai Liu
More multipurpose and convenient demand driven by Radio Frequency Identification (RFID) and intelligent packaging require flexible power sources. A VO₂(B)/graphene (VO₂(B)/GN) core-shell composite was successfully synthesized by the hydrothermal treatment with V₂O₅ and graphite. The as-obtained sample was characterized by XRD, FT-IR, SEM, TEM, and XPS measurements. In addition, the electrochemical properties of VO₂(B)/GN were tested. Due to its great electrochemical performance and mechanical properties, graphene could increase the electrochemical performance and strengthen the structural stability of the material at the same time...
December 7, 2018: Nanomaterials
https://www.readbyqxmd.com/read/30543042/health-benefits-and-pharmacological-effects-of-porphyra-species
#5
REVIEW
Kalkooru L Venkatraman, Alka Mehta
Porphyra, one of the most cultured red algae has gained economic importance across the globe for its nutritional benefits. Porphyra is being cultivated, harvested, dried, processed and consumed in large quantities in south eastern countries. It contains relatively high amounts of proteins, carbohydrates, and micronutrients. Exploitation of its fundamental attributes led to the discovery of various biologically active compounds like polysaccharides, phycobiliproteins and peptides with effective pharmacological applications...
December 13, 2018: Plant Foods for Human Nutrition
https://www.readbyqxmd.com/read/30542760/red-yeast-rice-fermentation-with-bacillus-subtilis-b2-under-blue-light-emitting-diodes-increases-antioxidant-secondary-products-manuscript-id-bpbse-18-0387
#6
Punniyakotti Elumalai, Yool-Jin Park, Min Cho, Patrick J Shea, Byung-Taek Oh
Light and bacteria can be used in combination to enhance secondary metabolite production during fermentation. Red yeast rice powder (RYRP) was inoculated with Bacillus subtilis (B2) isolated from freshwater seafood and incubated under light-emitting diodes (LEDs) of different colors (blue, green, red, white), fluorescent white light, and in darkness. Blue LED-mediated fermentation with B2 significantly enhanced production of phenolic compounds (68.4 ± 1 mg GAE/g DW) and flavonoids (51.7 ± 1 mg QE/g DW) compared to white light and darkness...
December 12, 2018: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/30541618/the-absence-of-retinal-input-disrupts-the-development-of-cholinergic-brainstem-projections-in-the-mouse-dorsal-lateral-geniculate-nucleus
#7
Guela Sokhadze, Tania A Seabrook, William Guido
BACKGROUND: The dorsal lateral geniculate nucleus (dLGN) of the mouse has become a model system for understanding thalamic circuit assembly. While the development of retinal projections to dLGN has been a topic of extensive inquiry, how and when nonretinal projections innervate this nucleus remains largely unexplored. In this study, we examined the development of a major nonretinal projection to dLGN, the ascending input arising from cholinergic neurons of the brainstem. To visualize these projections, we used a transgenic mouse line that expresses red fluorescent protein exclusively in cholinergic neurons...
December 12, 2018: Neural Development
https://www.readbyqxmd.com/read/30539787/enhancer-of-zeste-homologue-2-inhibition-attenuates-tgf-%C3%AE-dependent-hepatic-stellate-cell-activation-and-liver-fibrosis
#8
Rosa Martin-Mateos, Thiago M De Assuncao, Juan Pablo Arab, Nidhi Jalan-Sakrikar, Usman Yaqoob, Thomas Greuter, Vikas K Verma, Angela J Mathison, Sheng Cao, Gwen Lomberk, Philippe Mathurin, Raul Urrutia, Robert C Huebert, Vijay H Shah
BACKGROUND & AIMS: Transdifferentiation of hepatic stellate cells (HSCs) into myofibroblasts is a key event in the pathogenesis of liver fibrosis. Transforming growth factor β (TGF-β) and platelet-derived growth factor (PDGF) are canonical HSC activators after liver injury. The aim of this study was to analyze the epigenetic modulators that differentially control TGF-β and PDGF signaling pathways. METHODS: We performed a transcriptomic comparison of HSCs treated with TGF-β or PDGF-BB using RNA sequencing...
September 15, 2018: Cellular and Molecular Gastroenterology and Hepatology
https://www.readbyqxmd.com/read/30539403/ethylene-response-factors-pp4erf24-and-pp12erf96-regulate-blue-light-induced-anthocyanin-biosynthesis-in-red-zaosu-pear-fruits-by-interacting-with-myb114
#9
Junbei Ni, Songling Bai, Yuan Zhao, Minjie Qian, Ruiyan Tao, Lei Yin, Ling Gao, Yuanwen Teng
Pp4ERF24 and Pp12ERF96 fine tune blue light-induced anthocyanin biosynthesis via interacting with PpMYB114 and promoting the interaction between PpMYB114 and PpbHLH3, which enhances the expression of PpMYB114-induced PpUFGT. The red coloration of pear fruit is attributed to anthocyanin accumulation, which is transcriptionally regulated by the MYB-bHLH-WD40 complex. A number of ethylene response factors (ERF) have been identified to regulate anthocyanin biosynthesis in different plants. In pear, several ERF transcription factor genes were identified to be potentially involved in the light-induced anthocyanin biosynthesis according to transcriptome data...
December 11, 2018: Plant Molecular Biology
https://www.readbyqxmd.com/read/30538708/human-v%C3%AE-9v%C3%AE-2-t-lymphocytes-in-the-immune-response-to-p-falciparum-infection
#10
REVIEW
Jennifer Howard, Irfan Zaidi, Séverine Loizon, Odile Mercereau-Puijalon, Julie Déchanet-Merville, Maria Mamani-Matsuda
Malaria is an infectious disease caused by the protozoan parasite Plasmodium sp , the most lethal being Plasmodium falciparum . Clinical malaria is associated with the asexual replication cycle of Plasmodium parasites inside the red blood cells (RBCs) and a dysregulated immune response. Although the mechanisms of immune responses to blood-or liver-stage parasites have been extensively studied, this has not led to satisfactory leads for vaccine design. Among innate immune cells responding to infection are the non-conventional gamma-delta T-cells...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/30536937/moisture-resistant-mn4-doped-core-shell-structured-fluoride-red-phosphor-exhibiting-high-luminous-efficacy-for-warm-white-leds
#11
Decai Huang, Haomiao Zhu, Zhonghua Deng, Qilin Zou, Hongyu Lu, Xiaodong Yi, Wang Guo, Canzhong Lu, Xueyuan Chen
K2TiF6:Mn4+ is a highly efficient narrow-band emission red phosphor with promising applications in white LEDs and wide-gamut displays. Nevertheless, the poor moisture-resistant property of this material hinders its further commercialization. Herein, we introduce a convenient reverse cation exchange strategy to construct a core-shell structured K2TiF6:Mn4+@K2TiF6 phosphor. The outer pure K2TiF6 shell can not only act as a shield for preventing moisture in the air to hydrolyze inner MnF62- group, but also effectively cut off the path of energy migration to surface defects, thus increasing the emission efficiency, especially for the phosphors doped with high Mn4+ concentration...
December 11, 2018: Angewandte Chemie
https://www.readbyqxmd.com/read/30536210/improvement-of-blood-culture-contamination-rate-blood-volume-and-true-positive-rate-after-introducing-a-dedicated-phlebotomy-team
#12
Moonsuk Bae, Hae In Kim, Joung Ha Park, Byung-Han Ryu, Jeonghyun Chang, Heungsup Sung, Jiwon Jung, Min Jae Kim, Sung-Han Kim, Sang-Oh Lee, Sang-Ho Choi, Yang Soo Kim, Jun Hee Woo, Mi-Na Kim, Yong Pil Chong
The introduction of dedicated phlebotomy teams certified for blood collection has been reported to be highly cost-effective by reducing contamination rates. However, data on their effects on blood volume and true positive rate are limited. Therefore, we investigated the effect of replacing interns with a phlebotomy team on blood culture results. We performed a 24-month retrospective, quasi-experimental study before and after the introduction of a phlebotomy team dedicated to collecting blood cultures in a 2700-bed tertiary-care hospital...
December 7, 2018: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/30530929/diagnosis-and-treatment-of-multi-species-fish-mortality-attributed-to-enteromyxum-leei-while-in-quarantine-at-a-us-aquarium
#13
Michael W Hyatt, Thomas B Waltzek, Elizabeth A Kieran, Salvatore Frasca, Jan Lovy
Enteromyxum leei is an enteric myxozoan parasite of fish. This myxozoan has low host specificity and is the causative agent of myxozoan emaciation disease, known for heavy mortalities and significant financial losses within Mediterranean, Red Sea, and Asian aquaculture industries. The disease has rarely been documented within public aquaria and, to our knowledge, has never been confirmed within the USA. This case report describes an outbreak of E. leei in a population of mixed-species east African/Indo-Pacific marine fish undergoing quarantine at a public aquarium within the USA...
December 11, 2018: Diseases of Aquatic Organisms
https://www.readbyqxmd.com/read/30529413/photolysis-and-photocatalysis-of-tetracycline-by-sonochemically-heterojunctioned-bivo-4-reduced-graphene-oxide-under-visible-light-irradiation
#14
Tayyebeh Soltani, Ahmad Tayyebi, Byeong-Kyu Lee
The widespread use of antibiotics in pharmaceutical therapies and agricultural practice has led to severe environmental pollution. In this study, the simultaneous photolysis and photocatalysis behaviors of tetracycline (TC), one of the most frequently prescribed groups of antibiotics, were investigated using BiVO4 (BVO) supported on reduced graphene oxide (rGO). The resulting BVO/rGO nanocomposite (NC) showed prominent adsorption performance and photocatalytic ability under wide initial pH conditions (from acidic to alkaline: pH 2...
December 4, 2018: Journal of Environmental Management
https://www.readbyqxmd.com/read/30526586/extract-of-paecilomyces-hepiali-mycelia-induces-lipolysis-through-pka-mediated-phosphorylation-of-hormone-sensitive-lipase-and-erk-mediated-downregulation-of-perilipin-in-3t3-l1-adipocytes
#15
Mei Ge, Rui Guo, Hai-Xia Lou, Wen Zhang
BACKGROUND: Cordyceps sinensis has been used for centuries in China as one of the most valued herbal medicine and tonic food. Paecilomyces hepiali, a fungal strain isolated from natural C. sinensis, has been used widely as a substitute of C. sinensis in medicine and health food. P. hepiali has been reported to have various pharmaceutical benefits, including triglyceride-lowing activity. However, its effects on triglyceride metabolism in adipocytes remain unknown. The purpose of the present study was to evaluate the effect of P...
December 7, 2018: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/30525020/new-pim-1-kinase-inhibitor-from-the-co-culture-of-two-sponge-associated-actinomycetes
#16
Seham S El-Hawary, Ahmed M Sayed, Rabab Mohammed, Mohammad A Khanfar, Mostafa E Rateb, Tarek A Mohammed, Dina Hajjar, Hossam M Hassan, Tobias A M Gulder, Usama Ramadan Abdelmohsen
Saccharomonospora sp. UR22 and Dietzia sp. UR66, two actinomycetes derived from the Red Sea sponge Callyspongia siphonella , were co-cultured and the induced metabolites were monitored by HPLC-DAD and TLC. Saccharomonosporine A ( 1 ), a novel brominated oxo-indole alkaloid, convolutamydine F ( 2 ) along with other three known induced metabolites ( 3-5 ) were isolated from the EtOAc extract of Saccharomonospora sp. UR22 and Dietzia sp. UR66 co-culture. Additionally, axenic culture of Saccharomonospora sp. UR22 led to isolation of six known microbial metabolites ( 6-11 )...
2018: Frontiers in Chemistry
https://www.readbyqxmd.com/read/30523924/design-and-fabrication-of-bi-functional-tio-sub-2-sub-al-sub-2-sub-o-sub-3-sub-nanolaminates-with-selected-light-extraction-and-reliable-moisture-vapor-barrier-performance
#17
Yalian Weng, Guixiong Chen, Xiongtu Zhou, Qun Frank Yan, Tailiang Guo, Yongai Zhang
Bi-functional thin film with both selected light extraction and reliable moisture vapor barrier was proposed for simultaneous light management and encapsulation in the fields of lighting and display. Atomic layer deposition (ALD) was employed to obtain TiO2 and Al2O3 films with high uniformity, forming distributed Bragg reflector (DBR) structure. The DBRs exhibited excellent and tunable optical properties, as well as reliable moisture barrier performance. With increasing the DBR layers, the transmittances decreased obviously...
November 29, 2018: Nanotechnology
https://www.readbyqxmd.com/read/30521902/polysaccharide-from-a-tunisian-red-seaweed-chondrus-canaliculatus-structural-characteristics-antioxidant-activity-and-in-vivo-hemato-nephroprotective-properties-on-maneb-induced-toxicity
#18
Imen Jaballi, Imen Sallem, Amal Feki, Boutheina Cherif, Choumous Kallel, Ons Boudawara, Kamel Jamoussi, Lotfi Mellouli, Moncef Nasri, Ibtissem Ben Amara
The present study aims to investigate the physicochemical and the structural features of polysaccharide isolated from the red marine macro alga Chondrus canaliculatus (C.C.P) using FT-IR, gel filtration high-pressure chromatograph, HPLC-FID and solid state 13 C NMR analysis. C.C.P was even more tested in vitro for its potential antioxidant properties and in vivo for its hemato-nephroprotective effects against fungicide - maneb (MB) - induced toxicity. Animals treated for 20 days were allocated into six groups per six rats each: group 1 served as vehicle control, group 2 received MB, group 3 received MB + C...
December 3, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/30515330/virulence-of-aerial-conidia-of-beauveria-bassiana-produced-under-led-light-to-ctenocephalides-felis-cat-flea
#19
Sarayut Pittarate, Malee Thungrabeab, Supamit Mekchay, Patcharin Krutmuang
Ctenocephalides felis is an ectoparasitic flea species commonly found on dogs and cats. The current study verified the in vitro virulence of conidia of the entomopathogenic fungus Beauveria bassiana produced under different color LED light (red, blue, purple, green, yellow, and white) to adults of C. felis . The fungal isolates were cultivated on malt extract agar (MEA). Bioassay treatments used aerial conidia in test tubes. Adult fleas were obtained from a house cat in Chiang Mai province, Thailand. The experiments were composed of one control and eleven treatment groups...
2018: Journal of Pathogens
https://www.readbyqxmd.com/read/30507156/highly-efficient-chemically-stable-uv-blue-light-excitable-biluminescent-security-ink-to-combat-counterfeiting
#20
Akhilesh Kumar Singh, Satbir Singh, Bipin Kumar Gupta
A strategy has been demonstrated to design a biluminescent security ink using Eu(TTA)3Phen (ETP) and fluorescein for protecting the currency and other essential documents viz. passport, bank cheque, certificates against counterfeiting. Biluminescent security ink exhibits strong red and green emission under 367 and 445 nm excitations, respectively. As it is quite challenging to prepare a material that possesses two prominent (green and red) and distinguishable colors upon excitation with two separate LEDs sources, emitting at different wavelengths, the biluminescent security ink would be hard to counterfeit as compared to the existing luminescent security ink that exhibits single color under UV light exposure...
December 3, 2018: ACS Applied Materials & Interfaces
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