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https://www.readbyqxmd.com/read/28344726/evaluation-of-disposable-microfluidic-chip-design-for-automated-and-fast-immunoassays
#1
Guochun Wang, Champak Das, Bradley Ledden, Qian Sun, Chien Nguyen, Sai Kumar
We report here, the design and development of a disposable immunoassay chip for protein biomarker detection within ∼1 h. The unique design allows for real-time dynamic calibration of immunoassay for multiple biomarker detections on the chip. The limit of detection achieved for this test chip is 10 pg/ml for IL6, and 50 pg/ml for GFAP with a detection time of 1 h. The prototype instrument used for flowing the reagents through the chip can be easily assembled from off-the-shelf components with the final chemiluminescent detection carried out in a commercial plate reader...
January 2017: Biomicrofluidics
https://www.readbyqxmd.com/read/28336861/modified-continuum-mechanics-modeling-on-size-dependent-properties-of-piezoelectric-nanomaterials-a-review
#2
REVIEW
Zhi Yan, Liying Jiang
Piezoelectric nanomaterials (PNs) are attractive for applications including sensing, actuating, energy harvesting, among others in nano-electro-mechanical-systems (NEMS) because of their excellent electromechanical coupling, mechanical and physical properties. However, the properties of PNs do not coincide with their bulk counterparts and depend on the particular size. A large amount of efforts have been devoted to studying the size-dependent properties of PNs by using experimental characterization, atomistic simulation and continuum mechanics modeling with the consideration of the scale features of the nanomaterials...
January 26, 2017: Nanomaterials
https://www.readbyqxmd.com/read/28328070/modeling-and-simulation-of-dielectrophoretic-collective-dynamics-in-a-suspension-of-polarizable-particles-under-the-action-of-a-gradient-ac-electric-field
#3
Shigeru Tada, Yan Shen, Zhiyong Qiu
When a suspension of polarizable particles is subjected to a gradient AC electric field, the particles exhibit collective motion due to an interaction between the dipole induced in the particles and the spatial gradient of the electric field; this is known as dielectrophoresis. In the present study, the collective dynamics of suspended particles in a parallel-plate electric chamber was investigated by simulating numerically the trajectories of individual particles under the action of combined dielectrophoretic and dipole-dipole interparticle forces...
March 22, 2017: Electrophoresis
https://www.readbyqxmd.com/read/28323933/research-resource-transcriptional-dynamics-of-cultured-human-villous-cytotrophoblasts
#4
Joshua F Robinson, Mirhan Kapidzic, Matthew Gormley, Katherine Ona, Terrence Dent, Helia Seifikar, Emily G Hamilton, Susan J Fisher
During human pregnancy, cytotrophoblasts (CTBs) play key roles in uterine invasion, vascular remodeling, and anchoring of the feto-placental unit. Due to the challenges associated with studying human placentation in utero, cultured primary villous CTBs are used as a model of the differentiation pathway that leads to invasion of the uterine wall. In vitro, CTBs emulate in vivo cell behaviors, such as migration, aggregation and substrate penetration. While some of the molecular features related to these cell behaviors have been described, the underlying mechanisms, at a global level, remain undefined at mid-gestation...
March 8, 2017: Endocrinology
https://www.readbyqxmd.com/read/28315254/live-cell-imaging-of-protease-activity-assays-to-screen-therapeutic-approaches
#5
Anita Chalasani, Kyungmin Ji, Mansoureh Sameni, Samia H Mazumder, Yong Xu, Kamiar Moin, Bonnie F Sloane
Methodologies to image and quantify the activity of proteolytic enzymes have been developed in an effort to identify protease-related druggable pathways that are involved in malignant progression of cancer. Our laboratory has pioneered techniques for functional live-cell imaging of protease activity in pathomimetic avatars for breast cancer. We analyze proteolysis in the context of proliferation and formation of structures by tumor cells in 3-D cultures over time (4D). In order to recapitulate the cellular composition and architecture of tumors in the pathomimetic avatars, we include other tumor-associated cells (e...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28314909/localization-of-dynamin-related-protein-1-and-its-potential-role-in-lamellipodia-formation
#6
Youhwa Jo, Hyo Min Cho, Woong Sun, Jae Ryun Ryu
Dynamin-related protein1 (Drp1) plays an essential role in mitochondrial fission: Cytosolic Drp1 is translocated to the mitochondria upon stimulus, and oligomerized Drp1 constricts mitochondria by aid of actin filaments. Drp1 completes the fission process with GTP hydrolysis by its own GTPase activity. The importance of actin filament and its interaction with Drp1 in the mitochondrial fission process have been demonstrated. In this study, we found that Drp1 is enriched in the actin-rich leading edge of lamellipodia of mouse embryonic fibroblasts (MEFs) wherein mitochondria or peroxisomes are absent...
March 17, 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/28314109/a-dynamic-survival-detection-and-analysis-system-for-mosquito-larvae-viability-in-drug-assays
#7
Biao Guo, Yuesheng Zhu, Guibo Luo, Xiaorong Zuo
Bioinformatics studies have emerged in the domain of larval behavior analysis in recent years. A dynamic survival detection and analysis system for automatically monitoring a large amount of mosquito larvae in bioassays with multiwell plates by acquiring and processing videos is proposed in this article. In our system, equipment is designed for acquiring the video of the mosquito larvae in several multiwell plates simultaneously by a camera, and a video analysis module is developed for detecting the survival states of larvae in each well in real time...
March 1, 2017: SLAS Technol
https://www.readbyqxmd.com/read/28303130/new-approaches-on-quantification-of-campylobacter-jejuni-in-poultry-samples-the-use-of-digital-pcr-and-real-time-pcr-against-the-iso-standard-plate-count-method
#8
Bojan Papić, Mateja Pate, Urška Henigman, Urška Zajc, Igor Gruntar, Majda Biasizzo, Matjaž Ocepek, Darja Kušar
Campylobacteriosis is the most frequently reported bacterial food-borne illness in the European Union and contaminated broiler meat is considered the most important source of infection in humans. The aim of the present study was to evaluate real-time PCR (qPCR) and digital PCR (dPCR) for quantification of Campylobacter jejuni in 75 broiler neck-skin samples collected from a poultry slaughterhouse, and to compare them with the ISO 10272-2 standard plate count method. For qPCR standard curve, C. jejuni-negative neck-skin samples were spiked with C...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28302766/csf-clearance-in-alzheimer-disease-measured-with-dynamic-pet
#9
Mony J de Leon, Yi Li, Nobuyuki Okamura, Wai H Tsui, Les A Saint Louis, Lidia Glodzik, Ricardo S Osorio, Juan Fortea, Tracy Butler, Elizabeth Pirraglia, Silvia Fossati, Hee-Jin Kim, Roxana O Carare, Maiken Nedergaard, Helene Benveniste, Henry Rusinek
Evidence supporting the hypothesis that reduced cerebrospinal fluid (CSF) clearance is involved in the pathophysiology of Alzheimer's disease (AD) comes from primarily from rodent models. However, unlike rodents where predominant extra-cranial CSF egress is via olfactory nerves traversing the cribriform plate, human CSF clearance pathways are not well characterized. Using dynamic Positron Emission Tomography (PET) with (18)F-THK5117 a tracer for tau pathology, the ventricular CSF time activity was used as a biomarker for CSF clearance...
March 16, 2017: Journal of Nuclear Medicine: Official Publication, Society of Nuclear Medicine
https://www.readbyqxmd.com/read/28297883/simple-empirical-model-for-identifying-rheological-properties-of-soft-biological-tissues
#10
Yo Kobayashi, Mariko Tsukune, Tomoyuki Miyashita, Masakatsu G Fujie
Understanding the rheological properties of soft biological tissue is a key issue for mechanical systems used in the health care field. We propose a simple empirical model using fractional dynamics and exponential nonlinearity (FDEN) to identify the rheological properties of soft biological tissue. The model is derived from detailed material measurements using samples isolated from porcine liver. We conducted dynamic viscoelastic and creep tests on liver samples using a plate-plate rheometer. The experimental results indicated that biological tissue has specific properties: (i) power law increase in the storage elastic modulus and the loss elastic modulus of the same slope; (ii) power law compliance (gain) decrease and constant phase delay in the frequency domain; (iii) power law dependence between time and strain relationships in the time domain; and (iv) linear dependence in the low strain range and exponential law dependence in the high strain range between stress-strain relationships...
February 2017: Physical Review. E
https://www.readbyqxmd.com/read/28296566/a-new-elisa-based-method-for-detection-of-o-glcnac-transferase-activity-in-vitro
#11
Jieqiong Qi, Ruihong Wang, Yazhen Zeng, Wengong Yu, Yuchao Gu
O-GlcNAcylation is a dynamic, reversible, post-translational modification that regulates many cellular processes. O-GlcNAc transferase (OGT) is the sole enzyme transferring N-acetylglucosamine from UDP-GlcNAc to selected Ser/Thr residues of cytoplasm and nucleus proteins. Aberrant of OGT activity is associated with a number of diseases, suggesting OGT as a novel therapeutic target. In this study, we created a new ELISA-based method for detection of OGT activity. First, CKII, a well-known OGT substrate, was coated onto ELISA plate...
March 15, 2017: Preparative Biochemistry & Biotechnology
https://www.readbyqxmd.com/read/28296316/a-cross-sectional-study-of-the-radiation-dose-and-image-quality-of-x-ray-equipment-used-in-ivr
#12
Yohei Inaba, Koichi Chida, Ryota Kobayashi, Masayuki Zuguchi
There are case reports of injuries caused by the radiation from interventional radiology (IVR) X-ray systems. Therefore, the management of radiation doses in IVR is important. However, no detailed report has evaluated image quality for a large number of IVR X-ray systems. As a result, it is unclear whether the image quality of the X-ray equipment currently used in IVR procedures is optimal. We compared the entrance surface doses and image quality of multiple IVR X-ray systems. This study was conducted in 2014 at 13 medical facilities using 18 IVR X-ray systems...
July 2016: Journal of Applied Clinical Medical Physics
https://www.readbyqxmd.com/read/28293885/quantitative-and-dynamic-imaging-of-atm-kinase-activity
#13
Shyam Nyati, Grant Young, Brian Dale Ross, Alnawaz Rehemtulla
Ataxia telangiectasia mutated (ATM) is a serine/threonine kinase critical to the cellular DNA-damage response, including DNA double-strand breaks (DSBs). ATM activation results in the initiation of a complex cascade of events facilitating DNA damage repair, cell cycle checkpoint control, and survival. Traditionally, protein kinases have been analyzed in vitro using biochemical methods (kinase assays using purified proteins or immunological assays) requiring a large number of cells and cell lysis. Genetically encoded biosensors based on optical molecular imaging such as fluorescence or bioluminescence have been developed to enable interrogation of kinase activities in live cells with a high signal to background...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28283714/immobilized-metal-affinity-cryogel-based-high-throughput-platform-for-screening-bioprocess-and-chromatographic-parameters-of-his6-gtpase
#14
Joyita Sarkar, Ashok Kumar
Among various tools of product monitoring, chromatography is of vital importance as it also extends to the purification of product. Immobilized metal affinity cryogel (Cu(II)-iminodiacetic acid- and Ni(II)-nitrilotriacetic acid-polyacrylamide) minicolumns (diameter 8 mm, height 4 mm, void volume 250 μl) were inserted in open-ended 96-well plate and different chromatographic parameters and bioprocess conditions were analysed. The platform was first validated with lysozyme. Optimum binding of lysozyme (∼90%) was achieved when 50 μg of protein in 20 mM Tris, pH 8...
April 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28281266/a-smartphone-based-colorimetric-reader-for-human-c-reactive-protein-immunoassay
#15
A G Venkatesh, Thomas van Oordt, E Marion Schneider, Roland Zengerle, Felix von Stetten, John H T Luong, Sandeep Kumar Vashist
A smartphone-based colorimetric reader (SBCR), comprising a Samsung Galaxy SIII mini, a gadget (iPAD mini, iPAD4, or iPhone 5s) and a custom-made dark hood and base holder assembly, is used for human C-reactive protein (CRP) immunoassay. A 96-well microtiter plate (MTP) is positioned on the gadget's screensaver to provide white light-based bottom illumination only in the specific regions corresponding to the well's bottom. The images captured by the smartphone's back camera are analyzed by a novel image processing algorithm...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28279923/protein-free-formation-of-bone-like-apatite-new-insights-into-the-key-role-of-carbonation
#16
Alix C Deymier, Arun K Nair, Baptiste Depalle, Zhao Qin, Kashyap Arcot, Christophe Drouet, Claude H Yoder, Markus J Buehler, Stavros Thomopoulos, Guy M Genin, Jill D Pasteris
The nanometer-sized plate-like morphology of bone mineral is necessary for proper bone mechanics and physiology. However, mechanisms regulating the morphology of these mineral nanocrystals remain unclear. The dominant hypothesis attributes the size and shape regulation to organic-mineral interactions. Here, we present data supporting the hypothesis that physicochemical effects of carbonate integration within the apatite lattice control the morphology, size, and mechanics of bioapatite mineral crystals. Carbonated apatites synthesized in the absence of organic molecules presented plate-like morphologies and nanoscale crystallite dimensions...
February 27, 2017: Biomaterials
https://www.readbyqxmd.com/read/28279126/further-insight-into-the-bioactivity-of-the-freshwater-sponge-ochridaspongia-rotunda
#17
Aleksandra Talevska, Boris Pejin, Tanja Beric, Slavisa Stankovic
CONTEXT: Bioprospection has become a dynamic scientific field that explores novel possibilities for the implementation of natural products in medicine and pharmacy. Compared to marine species from all kingdoms, freshwater species have been highly neglected. OBJECTIVE: This work focuses on the screening of acetylcholinesterase inhibitory (AChE) and mutagenic activities of the acetone extract (obtained by maceration) of the freshwater sponge Ochridaspongia rotunda Arndt (Malawispongiidae) in vitro...
December 2017: Pharmaceutical Biology
https://www.readbyqxmd.com/read/28275337/tir-domain-containing-adapter-inducing-interferon-%C3%AE-trif-is-essential-for-mptp-induced-dopaminergic-neuroprotection-via-microglial-cell-m1-m2-modulation
#18
Minghui Shan, Sen Lin, Shurong Li, Yuchen Du, Haixia Zhao, Huarong Hong, Ming Yang, Xi Yang, Yongmei Wu, Liyi Ren, Jiali Peng, Jing Sun, Hongli Zhou, Bingyin Su
Dynamic changes of two phenotypes of microglia, M1 and M2, are critically associated with the neurodegeneration of Parkinson's disease. However, the regulation of the M1/M2 paradigm is still unclear. In the MPTP induced neurodegeneration model, we examined the concentration of dopamine (DA) related metabolites and the survival of tyrosine hydroxylase (TH) positive cells in WT and Trif(-/-) mice. In in vitro experiments, MN9D cells were co-cultured with BV2 cells to mimic the animal experiments. Inhibition of TRIF aggravated TH+ cell loss, and DA-related metabolites decreased...
2017: Frontiers in Cellular Neuroscience
https://www.readbyqxmd.com/read/28271994/nkx2-5-marks-angioblasts-that-contribute-to-hemogenic-endothelium-of-the-endocardium-and-dorsal-aorta
#19
Lyad Zamir, Reena Singh, Elisha Nathan, Ralph Patrick, Oren Yifa, Yfat Yahalom-Ronen, Alaa A Arraf, Thomas M Schultheiss, Shengbao Suo, Jing-Dong Jackie Han, Guangdun Peng, Naihe Jing, Yuliang Wang, Nathan Palpant, Patrick Pl Tam, Richard P Harvey, Eldad Tzahor
Novel regenerative therapies may stem from deeper understanding of the mechanisms governing cardiovascular lineage diversification. Using enhancer mapping and live imaging in avian embryos, and genetic lineage tracing in mice, we investigated the spatio-temporal dynamics of cardiovascular progenitor populations. We show that expression of the cardiac transcription factor Nkx2.5 marks a mesodermal population outside of the cardiac crescent in the extraembryonic and lateral plate mesoderm, with characteristics of hemogenic angioblasts...
March 8, 2017: ELife
https://www.readbyqxmd.com/read/28269632/in-vitro-identification-of-shoulder-joint-and-muscle-dynamics-based-on-motion-capture-and-musculoskeletal-computation
#20
Akihiko Murai, Yusuke Kawano, Ko Ayusawa, Mitsunori Tada, Noboru Matsumura, Takeo Nagura
Dynamics properties of shoulder joint and muscle are experimentally identified under different musculoskeletal conditions for a digital human model with accurate dynamics. Passive swing motions of scapula and upper limb bones in cadaveric specimen with and without muscles are measured by an optical motion capture system. External forces that are applied to the scapula bone are simultaneously measured by a force plate. The dynamics identification process consists of 3 steps: 1) identify the inertial parameters of the cadaveric specimen with and without muscles respectively, 2) identify the viscosity of the glenohumeral joint from the specimen without muscles, and 3) identify the viscosity of the shoulder muscles from the specimen with muscles and the identified joint viscosity...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
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