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https://www.readbyqxmd.com/read/28634865/dynamics-of-tobacco-dna-topoisomerases-ii-in-cell-cycle-regulation-to-manage-topological-constrains-during-replication-transcription-and-mitotic-chromosome-condensation-and-segregation
#1
Badri Nath Singh, V Mohan Murali Achary, Varakumar Panditi, Sudhir K Sopory, Malireddy K Reddy
The topoisomerase II expression varies as a function of cell proliferation. Maximal topoisomerase II expression was tightly coupled to S phase and G2/M phase via both transcriptional and post-transcriptional regulation. Investigation in meiosis using pollen mother cells also revealed that it is not the major component of meiotic chromosomes, it seems to diffuse out once meiotic chromosomal condensation is completed. Synchronized tobacco BY-2 cell cultures were used to study the role of topoisomerase II in various stages of the cell cycle...
June 20, 2017: Plant Molecular Biology
https://www.readbyqxmd.com/read/28634341/terahertz-circular-airy-vortex-beams
#2
Changming Liu, Jinsong Liu, Liting Niu, Xuli Wei, Kejia Wang, Zhengang Yang
Vortex beams have received considerable research interests both in optical and millimeter-wave domain since its potential to be utilized in the wireless communications and novel imaging systems. Many well-known optical beams have been demonstrated to carry orbital angular momentum (OAM), such as Laguerre-Gaussian beams and high-order Bessel beams. Recently, the radially symmetric Airy beams that exhibit an abruptly autofocusing feature are also demonstrated to be capable of carrying OAM in the optical domain...
June 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28632501/evaluation-of-automatic-dose-rate-control-for-flat-panel-imaging-using-a-spatial-frequency-domain-figure-of-merit
#3
Michiel Dehairs, Hilde Bosmans, Walter Desmet, Nicholas W Marshall
Current automatic dose rate controls (ADRCs) of dynamic x-ray imaging systems adjust acquisition parameters in response to changes in patient thickness in order to achieve a constant signal level in the image receptor. This work compares a 3 parameter (3P) ADRC control to a more flexible 5-parameter (5P) method to meet this goal. A phantom composed of 15 composite poly(methyl) methacrylate (PMMA)/aluminium (Al) plates was imaged on a Siemens Artis Q dynamic system using standard 3P and 5P ADRC technique. Phantom thickness covered a water equivalent thickness range of 2...
June 20, 2017: Physics in Medicine and Biology
https://www.readbyqxmd.com/read/28632158/on-the-application-of-image-processing-methods-for-bubble-recognition-to-the-study-of-subcooled-flow-boiling-of-water-in-rectangular-channels
#4
Concepción Paz, Marcos Conde, Jacobo Porteiro, Miguel Concheiro
This work introduces the use of machine vision in the massive bubble recognition process, which supports the validation of boiling models involving bubble dynamics, as well as nucleation frequency, active site density and size of the bubbles. The two algorithms presented are meant to be run employing quite standard images of the bubbling process, recorded in general-purpose boiling facilities. The recognition routines are easily adaptable to other facilities if a minimum number of precautions are taken in the setup and in the treatment of the information...
June 20, 2017: Sensors
https://www.readbyqxmd.com/read/28632144/optimal-pitching-axis-location-of-flapping-wings-for-efficient-hovering-flight
#5
Qi Wang, J Goosen, Fred Van Keulen
Flapping wings can pitch passively about their pitching axes due to their flexibility, inertia, and aerodynamic loads. A shift in the pitching axis location can dynamically alter the aerodynamic loads, which in turn changes the passive pitching motion and the flight efficiency. Therefore, it is of great interest to investigate the optimal pitching axis for flapping wings to maximize the power efficiency during hovering flight. In this study, flapping wings are modeled as rigid plates with non-uniform mass distribution...
June 20, 2017: Bioinspiration & Biomimetics
https://www.readbyqxmd.com/read/28632060/the-collision-forces-and-lower-extremity-inter-joint-coordination-during-running
#6
Li-I Wang, Chin-Yi Gu, I-Lin Wang, Sheng-Wun Siao, Szu-Ting Chen
The purpose of this study was to compare the lower extremity inter-joint coordination of different collision forces runners during running braking phase. A dynamical system approach was used to analyse the inter-joint coordination parameters. Data were collected with six infra-red cameras and two force plates. According to the impact peak of the vertical ground reaction force, twenty habitually rearfoot-strike runners were categorised into three groups: high collision forces runners (HF group, n = 8), medium collision forces runners (MF group, n = 5), and low collision forces runners (LF group, n = 7)...
February 28, 2017: Sports Biomechanics
https://www.readbyqxmd.com/read/28631616/on-the-dynamics-of-perching-manoeuvres-with-low-aspect-ratio-planforms
#7
J N Fernando, D E Rival
The dynamics of a simple perching manoeuvre are investigated using circular and aspect-ratio-two elliptical flat plates, as abstractions of low-aspect-ratio planforms observed in highly-manoeuvrable birds. The perching kinematic investigated in this study involves a pitch-up motion from an angle of attack of [Formula: see text] to [Formula: see text], while simultaneously decelerating. This motion is defined by the shape change number, [Formula: see text], which acts as a measure of the relative contributions of added-mass and circulatory effects...
June 20, 2017: Bioinspiration & Biomimetics
https://www.readbyqxmd.com/read/28622442/muscular-basis-of-whisker-torsion-in-mice-and-rats
#8
Sebastian Haidarliu, Knarik Bagdasarian, Namrata Shinde, Ehud Ahissar
Whisking mammals move their whiskers in the rostrocaudal and dorsoventral directions with simultaneous rolling about their long axes (torsion). Whereas muscular control of the first two types of whisker movement was already established, the anatomic muscular substrate of the whisker torsion remains unclear. Specifically, it was not clear whether torsion is induced by asymmetrical operation of known muscles or by other largely unknown muscles. Here we report that mystacial pads of newborn and adult rats and mice contain oblique intrinsic muscles (OMs) that connect diagonally adjacent vibrissa follicles...
June 16, 2017: Anatomical Record: Advances in Integrative Anatomy and Evolutionary Biology
https://www.readbyqxmd.com/read/28620822/digital-imaging-information-technology-for-biospeckle-activity-assessment-relative-to-bacteria-and-parasites
#9
Evelio E Ramírez-Miquet, Humberto Cabrera, Hilda C Grassi, Efrén de J Andrades, Isabel Otero, Dania Rodríguez, Juan G Darias
This paper reports on the biospeckle processing of biological activity using a visualization scheme based upon the digital imaging information technology. Activity relative to bacterial growth in agar plates and to parasites affected by a drug is monitored via the speckle patterns generated by a coherent source incident on the microorganisms. We present experimental results to demonstrate the potential application of this methodology for following the activity in time. The digital imaging information technology is an alternative visualization enabling the study of speckle dynamics, which is correlated to the activity of bacteria and parasites...
June 15, 2017: Lasers in Medical Science
https://www.readbyqxmd.com/read/28620411/vicar-an-adaptive-and-landmark-free-registration-of-time-lapse-image-data-from-microfluidics-experiments
#10
Georges Hattab, Jan-Philip Schlüter, Anke Becker, Tim W Nattkemper
In order to understand gene function in bacterial life cycles, time lapse bioimaging is applied in combination with different marker protocols in so called microfluidics chambers (i.e., a multi-well plate). In one experiment, a series of T images is recorded for one visual field, with a pixel resolution of 60 nm/px. Any (semi-)automatic analysis of the data is hampered by a strong image noise, low contrast and, last but not least, considerable irregular shifts during the acquisition. Image registration corrects such shifts enabling next steps of the analysis (e...
2017: Frontiers in Genetics
https://www.readbyqxmd.com/read/28610582/behavioral-and-neural-adaptations-in-response-to-five-weeks-of-balance-training-in-older-adults-a-randomized-controlled-trial
#11
Jan Ruffieux, Audrey Mouthon, Martin Keller, Michael Wälchli, Wolfgang Taube
BACKGROUND: While the positive effect of balance training on age-related impairments in postural stability is well-documented, the neural correlates of such training adaptations in older adults remain poorly understood. This study therefore aimed to shed more light on neural adaptations in response to balance training in older adults. METHODS: Postural stability as well as spinal reflex and cortical excitability was measured in older adults (65-80 years) before and after 5 weeks of balance training (n = 15) or habitual activity (n = 13)...
June 13, 2017: Journal of Negative Results in Biomedicine
https://www.readbyqxmd.com/read/28606547/self-assembled-supermolecular-hydrogel-based-on-hydroxyethyl-cellulose-formation-in-vitro-release-and-bacteriostasis-application
#12
Nan Sun, Ting Wang, Xiufeng Yan
Self-assembly of cellulose-based hydrogel is a new supermolecular architecture with potential for biomedical applications. In this study, a novel cellulose-based, supermolecular self-assembled hydrogel (gel-(β)CDP-HEC) was studied, which was based on the host-guest interaction between hydrophobic lauryl side chains grafting on hydroxyethyl cellulose (HECC12) and the cavities in poly(β-cyclodextrin) (β-CDP). The critical concentrations of HECC12 and β-CDP should be both fixed at 30mgmL(-1) by the results of dynamic viscosity, rheological property and swelling ratio...
September 15, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28606433/plate-osteosynthesis-of-proximal-ulna%C3%A2-fractures-a-biomechanical-micromotion-analysis
#13
Michael Hackl, Katharina Mayer, Mareike Weber, Manfred Staat, Roger van Riet, Klaus Josef Burkhart, Lars Peter Müller, Kilian Wegmann
PURPOSE: Double plating has been promoted, in recent years, as an alternative treatment method for proximal ulna fractures. This study aimed to compare the biomechanical properties of double-plate osteosynthesis with posterior plate fixation using a novel investigational design utilizing a 3-dimensional camera system to analyze fracture micromotion. METHODS: Fourteen fresh-frozen specimens were available for this study. Mayo type IIA fractures of the olecranon were created and internal fixation was performed with either an angular stable posterior plate or angular stable double plates...
June 9, 2017: Journal of Hand Surgery
https://www.readbyqxmd.com/read/28606334/an-investigation-into-the-cause-of-the-inner-dark-areas-and-outer-lighter-areas-ghosting-seen-in-dynamically-created-two-dimensional-bare-footprints
#14
Wesley Vernon Obe, Neil Simmonite, Sarah Reel, Selina Reidy
Dynamic bare footprints differ from static bare footprints through the presence of additional, lighter markings around the rear of the heel print and apices of the toe print areas. These images can appropriately be described as inner dark and outer ghosting features. To date, the functional cause of both features has not been understood. To gain such an understanding could potentially allow the further development and use of these features in forensic identification. The aim of this project was to investigate the causes of the inner dark and outer ghosting features seen in dynamic bare footprints through an observational, practice-based action research approach within a gait laboratory...
July 2017: Science & Justice: Journal of the Forensic Science Society
https://www.readbyqxmd.com/read/28605385/time-dependent-increase-in-the-network-response-to-the-stimulation-of-neuronal-cell-cultures-on-micro-electrode-arrays
#15
Monica L Gertz, Zachary Baker, Sharon Jose, Nathalia Peixoto
Micro-electrode arrays (MEAs) can be used to investigate drug toxicity, design paradigms for next-generation personalized medicine, and study network dynamics in neuronal cultures. In contrast with more traditional methods, such as patch-clamping, which can only record activity from a single cell, MEAs can record simultaneously from multiple sites in a network, without requiring the arduous task of placing each electrode individually. Moreover, numerous control and stimulation configurations can be easily applied within the same experimental setup, allowing for a broad range of dynamics to be explored...
May 29, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28602859/fibroblast-fibronectin-patterning-and-network-formation-in-3d-fibrin-matrices
#16
Miguel Miron-Mendoza, Eric Graham, Sujal Manohar, W Matthew Petroll
PURPOSE: We previously reported that fibroblasts migrating within 3-D collagen matrices move independently, whereas fibroblasts within 3-D fibrin matrices form an interconnected network. Similar networks have been identified previously during in vivo corneal wound healing. In this study, we investigate the role of fibronectin in mediating this mechanism of collective cell spreading, migration and patterning. METHODS: To assess cell spreading, corneal fibroblasts were plated within fibrillar collagen or fibrin matrices...
June 7, 2017: Matrix Biology: Journal of the International Society for Matrix Biology
https://www.readbyqxmd.com/read/28600315/rna-based-amplicon-sequencing-highlights-the-impact-of-the-processing-technology-on-microbiota-composition-of-artisanal-and-industrial-pdo-lard-d-arnad
#17
Ilario Ferrocino, Alberto Bellio, Angelo Romano, Guerrino Macori, Kalliopi Rantsiou, Lucia Decastelli, Luca Cocolin
"Valle d'Aosta Lard d'Arnad" is a protected designation of origin (PDO) product produced from fat of the shoulder and back of heavy pigs. Its manufacturing process can be very diverse, especially regarding the maturation temperature and the NaCl concentrationused for the brine, thereby the main goal of this study was to investigate the impact of those parameters on the microbiota developed during curing and ripening. Three farms producing Lard d'Arnad were selected. Two plants, reflecting the industrial process characterized either by low maturation temperature (plant A [10% NaCl; 2°C]) or by using a low NaCl concentrations (plant B [2...
June 9, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28597382/performance-and-microbial-community-dynamics-of-electricity-assisted-sequencing-batch-reactor-sbr-for-treatment-of-saline-petrochemical-wastewater
#18
Jiaxin Liu, Shengnan Shi, Xiangyu Ji, Bei Jiang, Lanlan Xue, Meidi Li, Liang Tan
High-salinity wastewater is often difficult to treat by common biological technologies due to salinity stress on the bacterial community. Electricity-assisted anaerobic technologies have significantly enhanced the treatment performance by alleviating the impact of salinity stress on the bacterial community, but electricity-assisted aerobic technologies have less been reported. Herein, a novel bio-electrochemistry system has been designed and operated in which a pair of stainless iron mesh-graphite plate electrodes were installed into a sequencing batch reactor (SBR, designated as S1) to strengthen the performance of saline petrochemical wastewater under aerobic conditions...
June 8, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28596780/katanin-effects-on-dynamics-of-cortical-microtubules-and-mitotic-arrays-in-arabidopsis-thaliana-revealed-by-advanced-live-cell-imaging
#19
George Komis, Ivan Luptovčiak, Miroslav Ovečka, Despina Samakovli, Olga Šamajová, Jozef Šamaj
Katanin is the only microtubule severing protein identified in plants so far. Previous studies have documented its role in regulating cortical microtubule organization during cell growth and morphogenesis. Although, some cell division defects are reported in KATANIN mutants, it is not clear whether or how katanin activity may affect microtubule dynamics in interphase cells, as well as the progression of mitosis and cytokinesis and the orientation of cell division plane (CDP). For this reason, we characterized microtubule organization and dynamics in growing and dividing cotyledon cells of Arabidopsis ktn1-2 mutant devoid of KATANIN 1 activity...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28590427/integral-design-methodology-of-photocatalytic-reactors-for-air-pollution-remediation
#20
REVIEW
Claudio Passalía, Orlando M Alfano, Rodolfo J Brandi
An integral reactor design methodology was developed to address the optimal design of photocatalytic wall reactors to be used in air pollution control. For a target pollutant to be eliminated from an air stream, the proposed methodology is initiated with a mechanistic derived reaction rate. The determination of intrinsic kinetic parameters is associated with the use of a simple geometry laboratory scale reactor, operation under kinetic control and a uniform incident radiation flux, which allows computing the local superficial rate of photon absorption...
June 7, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
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