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https://www.readbyqxmd.com/read/29350441/ultrasensitive-detection-of-shiga-toxin-2-and-its-variants-in-shiga-toxin-producing-escherichia-coli-strains-by-a-time-resolved-fluorescence-immunoassay
#1
Tian Wen, Chao Huang, Yi Zhang, Xiaoyan Zeng, Wendong Liu, Zhenbang Jiao, Xiling Guo, Yongjun Jiao
A rapid and sensitive two-step time-resolved fluorescence immunoassay (TRFIA) was developed for the detection of Shiga toxin 2 (Stx2) and its variants in Shiga toxin-producing Escherichia coli (STEC) strains. In sandwich mode, a monoclonal antibody against Stx2 was coated on a microtiter plate as a capture antibody. A tracer antibody against Stx2 labeled with europium(III) (Eu3+ ) chelate was then used as a detector, followed by fluorescence measurements using time-resolved fluorescence. The sensitivity of Stx2 detection was 0...
January 19, 2018: Luminescence: the Journal of Biological and Chemical Luminescence
https://www.readbyqxmd.com/read/29350182/the-role-of-eeg-in-the-diagnosis-and-classification-of-the-epilepsy-syndromes-a-tool-for-clinical-practice-by-the-ilae-neurophysiology-task-force-part-2
#2
Michalis Koutroumanidis, Alexis Arzimanoglou, Roberto Caraballo, Sushma Goyal, Anna Kaminska, Pramote Laoprasert, Hirokazu Oguni, Guido Rubboli, William Tatum, Pierre Thomas, Eugen Trinka, Luca Vignatelli, Solomon L Moshé
The concept of epilepsy syndromes, introduced in 1989, was defined as "clusters of signs and symptoms customarily occurring together". Definition of epilepsy syndromes based on electro-clinical features facilitated clinical practice and, whenever possible, clinical research in homogeneous groups of patients with epilepsies. Progress in the fields of neuroimaging and genetics made it rapidly clear that, although crucial, the electro-clinical description of epilepsy syndromes was not sufficient to allow much needed development of targeted therapies and a better understanding of the underlying pathophysiological mechanisms of seizures...
December 1, 2017: Epileptic Disorders: International Epilepsy Journal with Videotape
https://www.readbyqxmd.com/read/29348436/high-throughput-analysis-using-non-depletive-spme-challenges-and-applications-to-the-determination-of-free-and-total-concentrations-in-small-sample-volumes
#3
Ezel Boyacı, Barbara Bojko, Nathaly Reyes-Garcés, Justen J Poole, Germán Augusto Gómez-Ríos, Alexandre Teixeira, Beate Nicol, Janusz Pawliszyn
In vitro high-throughput non-depletive quantitation of chemicals in biofluids is of growing interest in many areas. Some of the challenges facing researchers include the limited volume of biofluids, rapid and high-throughput sampling requirements, and the lack of reliable methods. Coupled to the above, growing interest in the monitoring of kinetics and dynamics of miniaturized biosystems has spurred the demand for development of novel and revolutionary methodologies for analysis of biofluids. The applicability of solid-phase microextraction (SPME) is investigated as a potential technology to fulfill the aforementioned requirements...
January 18, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29347735/spreading-of-nonmotile-bacteria-on-a-hard-agar-plate-comparison-between-agent-based-and-stochastic-simulations
#4
Navdeep Rana, Pushpita Ghosh, Prasad Perlekar
We study spreading of a nonmotile bacteria colony on a hard agar plate by using agent-based and continuum models. We show that the spreading dynamics depends on the initial nutrient concentration, the motility, and the inherent demographic noise. Population fluctuations are inherent in an agent-based model, whereas for the continuum model we model them by using a stochastic Langevin equation. We show that the intrinsic population fluctuations coupled with nonlinear diffusivity lead to a transition from a diffusion limited aggregation type of morphology to an Eden-like morphology on decreasing the initial nutrient concentration...
November 2017: Physical Review. E
https://www.readbyqxmd.com/read/29347632/dynamics-of-a-thermally-driven-film-climbing-the-outside-of-a-vertical-cylinder
#5
Linda B Smolka
The dynamics of a film climbing the outside of a vertical cylinder under the competing effects of a thermally driven surface tension gradient and gravity is examined through numerical simulations of a thin-film model for the film height. The model, including boundary conditions, depends on three parameters, the scaled cylinder radius R[over ̂], the upstream film height h_{∞}, and the downstream precursor film thickness b, and reduces to the model for Marangoni driven film climbing a vertical plate in the limit R[over ̂]→∞...
October 2017: Physical Review. E
https://www.readbyqxmd.com/read/29347539/theory-of-nanobubble-formation-and-induced-force-in-nanochannels
#6
Noriyoshi Arai, Takahiro Koishi, Toshikazu Ebisuzaki
This paper presents a fundamental theory of nanobubble formation and induced force in confined nanochannels. It is shown that nanobubble formation between hydrophobic plates can be predicted from their surface tension and geometry, with estimated values for the surface free energy and the force acting on the plates in good agreement with the results of molecular dynamics simulation and experimentation. When a bubble is formed between two plates, vertical attractive force and horizontal retract force due to the shifted plates are applied to the plates...
October 2017: Physical Review. E
https://www.readbyqxmd.com/read/29347466/intermittency-and-emergence-of-coherent-structures-in-wave-turbulence-of-a-vibrating-plate
#7
Nicolas Mordant, Benjamin Miquel
We report numerical investigations of wave turbulence in a vibrating plate. The possibility to implement advanced measurement techniques and long-time numerical simulations makes this system extremely valuable for wave turbulence studies. The purely 2D character of dynamics of the elastic plate makes it much simpler to handle compared to much more complex 3D physical systems that are typical of geo- and astrophysical issues (ocean surface or internal waves, magnetized plasmas or strongly rotating and/or stratified flows)...
October 2017: Physical Review. E
https://www.readbyqxmd.com/read/29347460/boltzmann-kinetic-equation-for-a-strongly-confined-gas-of-hard-spheres
#8
J Javier Brey, M I García de Soria, P Maynar
A Boltzmann-like kinetic equation for a quasi-two-dimensional gas of hard spheres is derived. The system is confined between two parallel hard plates separated a distance between one and two particle diameters. An entropy Lyapunov function for the equation is identified. In addition to the usual Boltzmann expression, it contains a contribution associated to the confinement of the particles. The steady properties of the system agree with equilibrium statistical mechanics results. Equations describing the energy transfer between the degrees of freedom parallel and perpendicular to the confining plates are obtained for some simple initial configurations...
October 2017: Physical Review. E
https://www.readbyqxmd.com/read/29346994/weakly-non-boussinesq-convection-in-a-gaseous-spherical-shell
#9
Lydia Korre, Nicholas Brummell, Pascale Garaud
We examine the dynamics associated with weakly compressible convection in a spherical shell by running 3D direct numerical simulations using the Boussinesq formalism [Spiegel and Veronis, Astrophys. J. 131, 442 (1960)AJLEEY0004-637X10.1086/146849]. Motivated by problems in astrophysics, we assume the existence of a finite adiabatic temperature gradient ∇T_{ad} and use mixed boundary conditions for the temperature with fixed flux at the inner boundary and fixed temperature at the outer boundary. This setup is intrinsically more asymmetric than the more standard case of Rayleigh-Bénard convection in liquids between parallel plates with fixed temperature boundary conditions...
September 2017: Physical Review. E
https://www.readbyqxmd.com/read/29345852/cadmium-and-cadmium-tolerant-soil-bacteria-in-cacao-crops-from-northeastern-colombia
#10
Daniel Bravo, Sergio Pardo-Díaz, Javier Benavides-Erazo, Gersain Rengifo-Estrada, Olivier Braissant, Clara Leon-Moreno
AIMS: This research aims to assess total-cadmium soil content and microbiological aspects to understand the dynamics of culturable cadmium-tolerant bacteria (CdtB) in cacao soils from northeastern Colombia. METHODS AND RESULTS: An integration of inverted dish plating, Cd determination and a microcalorimetry assay (IMC) was carried out. A farm in Boyacá showed the highest level of total soil Cd (3.74 mg kg-1 ) followed by farms in Santander and Arauca (2.76 and 1...
January 18, 2018: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/29345472/current-driven-motion-of-domain-boundaries-between-skyrmion-lattice-and-helical-magnetic-structure
#11
Kiyou Shibata, Toshiaki Tanigaki, Tetsuya Akashi, Hiroyuki Shinada, Ken Harada, Kodai Niitsu, Daisuke Shindo, Naoya Kanazawa, Yoshinori Tokura, Taka-Hisa Arima
To utilize magnetic skyrmions, nano-scale vortex-like magnetic structure, experimental elucidation of their dynamics against current application in various circumstances such as in confined structure and mixture of different magnetic phases is indispensable. Here, we investigate the current-induced dynamics of the coexistence state of magnetic skyrmions and helical magnetic structure in a thin plate of B20-type helimagnet FeGe in terms of in-situ realspace observation using Lorentz transmission electron microscopy (TEM)...
January 18, 2018: Nano Letters
https://www.readbyqxmd.com/read/29343835/a-non-zircon-hf-isotope-record-in-archean-black-shales-from-the-pilbara-craton-confirms-changing-crustal-dynamics-ca-3-ga-ago
#12
Yona Nebel-Jacobsen, Oliver Nebel, Martin Wille, Peter A Cawood
Plate tectonics and associated subduction are unique to the Earth. Studies of Archean rocks show significant changes in composition and structural style around 3.0 to 2.5 Ga that are related to changing tectonic regime, possibly associated with the onset of subduction. Whole rock Hf isotope systematics of black shales from the Australian Pilbara craton, selected to exclude detrital zircon components, are employed to evaluate the evolution of the Archean crust. This approach avoids limitations of Hf-in-zircon analyses, which only provide input from rocks of sufficient Zr-concentration, and therefore usually represent domains that already underwent a degree of differentiation...
January 17, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29343646/role-of-boundary-conditions-in-determining-cell-alignment-in-response-to-stretch
#13
Kellen Chen, Andrea Vigliotti, Mattia Bacca, Robert M McMeeking, Vikram S Deshpande, Jeffrey W Holmes
The ability of cells to orient in response to mechanical stimuli is essential to embryonic development, cell migration, mechanotransduction, and other critical physiologic functions in a range of organs. Endothelial cells, fibroblasts, mesenchymal stem cells, and osteoblasts all orient perpendicular to an applied cyclic stretch when plated on stretchable elastic substrates, suggesting a common underlying mechanism. However, many of these same cells orient parallel to stretch in vivo and in 3D culture, and a compelling explanation for the different orientation responses in 2D and 3D has remained elusive...
January 17, 2018: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29342080/comparisons-of-different-models-on-dynamic-recrystallization-of-plate-during-asymmetrical-shear-rolling
#14
Tao Zhang, Lei Li, Shi-Hong Lu, Hai Gong, Yun-Xin Wu
Asymmetrical shear rolling with velocity asymmetry and geometry asymmetry is beneficial to enlarge deformation and refine grain size at the center of the thick plate compared to conventional symmetrical rolling. Dynamic recrystallization (DRX) plays a vital role in grain refinement during hot deformation. Finite element models (FEM) coupled with microstructure evolution models and cellular automata models (CA) are established to study the microstructure evolution of plate during asymmetrical shear rolling. The results show that a larger DRX fraction and a smaller average grain size can be obtained at the lower layer of the plate...
January 17, 2018: Materials
https://www.readbyqxmd.com/read/29341685/intermittent-granular-dynamics-at-a-seismogenic-plate-boundary
#15
Yasmine Meroz, Brendan J Meade
Earthquakes at seismogenic plate boundaries are a response to the differential motions of tectonic blocks embedded within a geometrically complex network of branching and coalescing faults. Elastic strain is accumulated at a slow strain rate on the order of 10^{-15}  s^{-1}, and released intermittently at intervals >100  yr, in the form of rapid (seconds to minutes) coseismic ruptures. The development of macroscopic models of quasistatic planar tectonic dynamics at these plate boundaries has remained challenging due to uncertainty with regard to the spatial and kinematic complexity of fault system behaviors...
September 29, 2017: Physical Review Letters
https://www.readbyqxmd.com/read/29339841/role-of-autologous-non-vascularised-intramedullary-fibular-strut-graft-in-humeral-shaft-nonunions-following-failed-plating
#16
Sreekanth Kashayi-Chowdojirao, Aashish Vallurupalli, Vijay Krishna Chilakamarri, Chandrasekhar Patnala, Lalith Mohan Chodavarapu, Nageswara Rao Kancherla, Asif Hussain Khazi Syed
Background: Non-union humeral shaft fractures are seen frequently in clinical practice at about 2-10% in conservative management and 30% in surgically operated patients. Osteosynthesis using dynamic compression plate (DCP), intramedullary nailing, locking compression plate (LCP), Ilizarov technique along with bone grafting have been reported previously. In cases of prior failed plate-screw osteosynthesis the resultant osteopenia, cortical defect, bone loss, scalloping around screws and metallosis, make the management of non-union more complicated...
November 2017: Journal of Clinical Orthopaedics and Trauma
https://www.readbyqxmd.com/read/29338819/in-vitro-efficacy-of-continuous-mild-high-temperature-on-the-biofilm-formation-of-aspergillus-niger
#17
Rong Zeng, Jian Bo Tong, Yu Zhen Liu, Qing Chen, Tong Lin, Min Li, Gui Xia Lü
Objective To investigate whether continuous mild high temperature (increased temperature without causing significant damage to host cells) can inhibit the biofilm formation of Aspergillus niger (A.niger) and its vitality.Methods A.niger biofilms were formed on a coverslip in 24-well tissue culture plate and were checked at the time points 4,8,10,16,24,48 and 72 hours.Confocal laser scanning microscopy (CLSM) was used to image and quantify A.niger biofilm formation under three different continuous mild high temperatures at 37℃,39℃,and 41℃...
December 20, 2017: Zhongguo Yi Xue Ke Xue Yuan Xue Bao. Acta Academiae Medicinae Sinicae
https://www.readbyqxmd.com/read/29338346/foot-mobility-magnitude-and-stiffness-in-children-with-and-without-calcaneal-apophysitis
#18
Simon Christopher McSweeney, Lloyd Reed, Scott Wearing
BACKGROUND: This study evaluated quasi-static measures of foot mobility magnitude (FMM) and foot stiffness (FS) in children, aged 8 to 14 years, with and without calcaneal apophysitis. METHODS: Between 2016 and 2017, FMM and FS measurements were captured on 41 children (22 cases and 19 controls) using a custom-built foot assessment platform. The platform incorporated a portable force plate that allowed quantification of vertical force during double-limb stance (DLS)...
January 1, 2018: Foot & Ankle International
https://www.readbyqxmd.com/read/29332501/-plates-and-dishes-smash-married-couples-clash-cultural-and-social-barriers-to-help-seeking-among-women-domestic-violence-survivors-in-kyrgyzstan
#19
Saltanat Childress
This article develops a grounded theory of help-seeking to investigate the social and cultural determinants of help-seeking among Kyrgyz women who have experienced domestic violence. Results indicate that cultural traditions and social norms-most notably the social construction of marriage, the shame associated with divorce, and the status of daughters-in-law in Kyrgyz society-are used to justify domestic violence and prevent victims from seeking help. The proposed theory and results suggest that scholars, policymakers, and front-line contacts must emphasize dispelling myths, misconceptions, and traditional beliefs about gender and marriage to break the abusive dynamics and provide professional help...
August 1, 2017: Violence Against Women
https://www.readbyqxmd.com/read/29332433/slippage-of-tightrope-button-in-syndesmotic-fixation-of-weber-c-malleolar-fractures-a-case-series
#20
Mas'uud Ibnu Samsudin, Ming Quan Wayne Yap, Ang Wei Luong, Ernest Beng Kee Kwek
BACKGROUND: Tightrope fixation is an emerging technique for syndesmotic fixation with promising results. However, our case series highlights the slippage of Tightrope buttons as a complication of suture button syndesmotic fixation of Weber C malleolar fractures using limited contact dynamic compression (LCDCP) plates. METHODS: We report a series of cases from our database in which slippage of the Tightrope button through the LCDCP holes in Weber C malleolar fractures was noted...
January 1, 2018: Foot & Ankle International
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