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https://www.readbyqxmd.com/read/29776032/polyelectrolyte-brush-bilayers-in-weak-interpenetration-regime-scaling-theory-and-molecular-dynamics-simulations
#1
Parth Rakesh Desai, Shayandev Sinha, Siddhartha Das
We employ molecular dynamics (MD) simulations and develop scaling theories to quantify the equilibrium behavior of polyelectrolyte (PE) brush bilayers (BBLs) in the weakly interpenetrated regime, which is characterized by d_{0}<d_{g}<2d_{0}, where d_{g} is the gap between the opposing plates where the PE brushes are grafted and d_{0} is the unperturbed height of a PE brush grafted at a single plate. Scaling predictions establish that, for the weakly interpenetrated osmotic PE BBLs δ∼N^{1/2}(2-d_{g}/d_{0})^{1/2} (where δ is the interpenetration length and N is the number of Kuhn segments in PE brush)...
March 2018: Physical Review. E
https://www.readbyqxmd.com/read/29774013/dynamics-of-aspen-roots-colonization-by-pseudomonads-reveals-strain-specific-and-mycorrhizal-specific-patterns-of-biofilm-formation
#2
Marie-Francoise Noirot-Gros, Shalaka Shinde, Peter E Larsen, Sarah Zerbs, Peter J Korajczyk, Kenneth M Kemner, Philippe H Noirot
Rhizosphere-associated Pseudomonas fluorescens are known plant growth promoting (PGP) and mycorrhizal helper bacteria (MHB) of many plants and ectomycorrhizal fungi. We investigated the spatial and temporal dynamics of colonization of mycorrhizal and non-mycorrhizal Aspen seedlings roots by the P. fluorescens strains SBW25, WH6, Pf0-1, and the P. protegens strain Pf-5. Seedlings were grown in laboratory vertical plates systems, inoculated with a fluorescently labeled Pseudomonas strain, and root colonization was monitored over a period of 5 weeks...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29768981/a-scalable-pipeline-for-high-throughput-flow-cytometry
#3
Aaron C Wilson, Ioannis K Moutsatsos, Gary Yu, Javier J Pineda, Yan Feng, Douglas S Auld
Flow cytometry (FC) provides high-content data for a variety of applications, including phenotypic analysis of cell surface and intracellular markers, characterization of cell supernatant or lysates, and gene expression analysis. Historically, sample preparation, acquisition, and analysis have presented as a bottleneck for running such types of assays at scale. This article will outline the solutions that have been implemented at Novartis which have allowed high-throughput FC to be successfully conducted and analyzed for a variety of cell-based assays...
May 1, 2018: SLAS Discovery
https://www.readbyqxmd.com/read/29768486/niche-partitioning-as-a-mechanism-for-locally-high-species-diversity-within-a-geographically-limited-genus-of-blastoid
#4
Ryan FitzGerald Morgan
Deltoblastus batheri and Deltoblastus delta occur concurrently in many Permian deposits from Timor. Closely related sister species living in direct proximity without alteration in feeding habit would be in direct violation of Lotka-Volterra dynamics. These two species were measured and compared to see if any evidence of differentiation along feeding lines has occurred in order to reduce direct competition. P-values obtained via Student's t test display significant differentiation across all measured parameters...
2018: PloS One
https://www.readbyqxmd.com/read/29765190/evaluating-equilibrium-in-anterior-cruciate-ligament-reconstruction
#5
REVIEW
Forough Ferdowsi, Zahra Sadat Rezaeian
[Purpose] The purpose of this review was to summarize available methods and equipment which are administered to evaluate the balance in anterior cruciate ligament reconstruction. [Subjects and Methods] A literature search was performed and reviewed using the narrative approach. This study reviewed English articles concerning balance assessment methods in anterior cruciate ligament reconstruction subjects from 1985 to 2015 using the following key words: Anterior Cruciate Ligament Injury, Anterior Cruciate Ligament Reconstruction, Postural Control, Equilibrium, Balance and Stability...
May 2018: Journal of Physical Therapy Science
https://www.readbyqxmd.com/read/29763813/age-and-gymnastic-experience-effects-on-sensory-reweighting-processes-during-quiet-stand
#6
Albert Busquets, Silvia Aranda-Garcia, Blai Ferrer-Uris, Michel Marina, Rosa Angulo-Barroso
BACKGROUND: The relative contribution of sensory inputs to control balance while standing is dynamically adjusted. These sensory reweighting processes could be impacted by age and sport expertise capabilities, especially when the sport emphasizes equilibrium like artistic gymnastics. RESEARCH QUESTION: The aim of this study was to explore the sensory reweighting processes to adjust standing posture in children and adults with different gymnastic expertise (gymnasts, G, and non-gymnast, NG)...
May 8, 2018: Gait & Posture
https://www.readbyqxmd.com/read/29759642/covering-the-instrument-table-decreases-bacterial-bioburden-an-evaluation-of-environmental-quality-indicators
#7
Troy A Markel, Thomas Gormley, Damon Greeley, John Ostojic, Jennifer Wagner
BACKGROUND: Covering the instrument table during surgery may decrease contamination. We hypothesized that (1) covering the instrument table in an operating room (OR) during static periods of nonuse and dynamic periods of active use would dramatically decrease the bacterial bioburden on the table, and (2) the use of sterile plastic table covers would be equivalent to sterile impervious paper covers in reducing the bioburden in a dynamic environment. METHODS: Bacterial contamination of the instrument table was evaluated by settle plates in static and dynamic ORs...
May 11, 2018: American Journal of Infection Control
https://www.readbyqxmd.com/read/29753125/adaptation-of-kinematic-synergy-and-postural-control-to-mechanical-ankle-constraint-on-an-unsteady-stance-surface
#8
Yi-Ying Tsai, Gwo-Ching Chang, Ing-Shiou Hwang
Joint constraint interferes with the coordinative structure in joint movements used to optimize postural stability. This study aimed to investigate changes in postural synergy when the ankle joints were bilaterally braced during a stabilometer stance. Twenty-four young adults stood on a stabilometer plate while wearing a pair of ankle-foot orthoses, which were either unlocked or locked to restrict ankle motion (the ankle constraint (AC) and non-constraint (NC) conditions). Although ankle constraint did not significantly affect the dynamics of the stabilometer movements, the size and regularity of the first principal component (PC1), which explained more than 80% of the variance of joint movements in the lower limb, were increased...
May 9, 2018: Human Movement Science
https://www.readbyqxmd.com/read/29752220/biomechanical-characteristics-of-biplane-multiplanar-tension-side-fixation-for-lapidus-fusion
#9
Paul Dayton, Daniel J Hatch, Robert D Santrock, Bret Smith
Although plating on the plantar, tension-side of the metatarsocuneiform joint provides an inherent biomechanical advantage for Lapidus arthrodesis, it has not been widely adopted owing to the morbidity associated with plantar application. To overcome these limitations, a modification to 90-90 locked biplanar plating was developed to provide the biomechanical advantages of multiplanar fixation and tension-side fixation, allowing application through a conventional incision. We tested the hypothesis that biplanar plating with tension-side fixation (low-profile straight dorsal plate and anatomic medial-plantar plate) would demonstrate improved mechanical stability compared with a previously tested 90-90 biplanar construct (small straight plate dorsally and medially) under cyclic loading...
May 8, 2018: Journal of Foot and Ankle Surgery: Official Publication of the American College of Foot and Ankle Surgeons
https://www.readbyqxmd.com/read/29746138/microarray-plate-method-for-estimation-of-precipitation-kinetics-of-celecoxib-under-biorelevant-conditions-and-precipitate-characterization
#10
Ikjot Sodhi, Abhay T Sangamwar
Study methodologies of supersaturated state are fast developing as pharmaceutical industry is adopting supersaturating drug delivery systems (SDDS) to overcome solubility issue of drugs. The 'parachute' of sobriquet 'spring-and-parachute' which indicates delayed or slowed intraluminal precipitation of drug from SDDS, is of immense importance to formulation scientists since optimal attainment of 'parachute' governs the success of SDDS in stabilizing supersaturated state that ensues in enhancement of bioavailability...
May 10, 2018: Molecular Pharmaceutics
https://www.readbyqxmd.com/read/29736584/bioreactors-and-microfluidics-for-osteochondral-interface-maturation
#11
Raphaël F Canadas, Alexandra P Marques, Rui L Reis, J Miguel Oliveira
The cell culture techniques are in the base of any biology-based science. The standard techniques are commonly static platforms as Petri dishes, tissue culture well plates, T-flasks, or well plates designed for spheroids formation. These systems faced a paradigm change from 2D to 3D over the current decade driven by the tissue engineering (TE) field. However, 3D static culture approaches usually suffer from several issues as poor homogenization of the formed tissues and development of a necrotic center which limits the size of in vitro tissues to hundreds of micrometers...
2018: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/29735385/does-lag-screw-fixation-of-condylar-fractures-result-in-adequate-stability-a-finite-element-analysis
#12
Ricardo Augusto Conci, Eleonor Álvaro Garbin, Geraldo Luiz Griza, Natasha Magro Érnica, Pedro Yoshito Noritomi, Flavio Henrique Silveira Tomazi, Guilherme Genehr Fritscher, Claiton Heitz
The great incidence and controversies related to the diagnosis, treatment, surgical accesses, and type of osteosynthesis materials confer an outstanding role to condylar fractures among facial fractures. Plate configurations, with diverse formats and sizes, may be used to surgically resolve condylar fractures. With the purpose of improving the advantages and minimizing the disadvantages of fixation techniques, the neck screw was developed aiming at the needed stabilization to render a correct fixation through a system of dynamic compression...
April 11, 2018: Journal of Cranio-maxillo-facial Surgery
https://www.readbyqxmd.com/read/29733310/ex-utero-electroporation-and-organotypic-slice-cultures-of-embryonic-mouse-brains-for-live-imaging-of-migrating-gabaergic-interneurons
#13
Lara Eid, Mathieu Lachance, Gilles Hickson, Elsa Rossignol
GABAergic interneurons (INs) are critical components of neuronal networks that drive cognition and behavior. INs destined to populate the cortex migrate tangentially from their place of origin in the ventral telencephalon (including from the medial and caudal ganglionic eminences (MGE, CGE)) to the dorsal cortical plate in response to a variety of intrinsic and extrinsic cues. Different methodologies have been developed over the years to genetically manipulate specific pathways and investigate how they regulate the dynamic cytoskeletal changes required for proper IN migration...
April 20, 2018: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29723629/oil-from-the-fruits-of-pterodon-emarginatus-vog-a-traditional-anti-inflammatory-study-combining-in-vivo-and-in-silico
#14
Cleydson Breno Rodrigues Dos Santos, Ryan da Silva Ramos, Brenda Lorena Sánchez Ortiz, Gabriel Monteiro da Silva, Silvana Giuliatti, José Luis Balderas-Lopez, Andrés Navarrete, José Carlos Tavares Carvalho
ETHNOBOTANICAL RELEVANCE: The oil obtained from the fruits of Pterodon emarginatus Vog. (OPe) is used orally and topically, in traditional medicine for some purposes, such as acute and chronic inflammatory states as rheumatoid arthritis. MATERIALS AND METHODS: In this work, the anti-inflammatory activity of the OPe was demonstrated based on several animal models and presented an in silico study based on the 6α,7β-dihydroxy-vouacapan-17β-oic acid (DHVA) majority compound of the OPe to evaluate the interaction this compound, with cyclooxygenase-2 (COX-2) in 4COX (Mus musculus) and 5KIR (Homo sapiens) and molecular dynamics simulation...
April 30, 2018: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/29714277/multiple-light-scattering-in-metallic-ejecta-produced-under-intense-shockwave-compression
#15
J-E Franzkowiak, P Mercier, G Prudhomme, L Berthe
A roughened metallic plate, subjected to intense shock wave compression, gives rise to an expanding ejecta particle cloud. Photonic Doppler velocimetry (PDV), a fiber-based heterodyne velocimeter, is often used to track ejecta velocities in dynamic compression experiments and on nanosecond time scales. Shortly after shock breakout at the metal-vacuum interface, a particular feature observed in many experiments in the velocity spectrograms is what appear to be slow-moving ejecta, below the free-surface velocity...
April 10, 2018: Applied Optics
https://www.readbyqxmd.com/read/29712790/nmda-receptor-signaling-is-important-for-neural-tube-formation-and-for-preventing-antiepileptic-drug-induced-neural-tube-defects
#16
Eduardo B Sequerra, Raman Goyal, Patricio A Castro, Jacqueline B Levin, Laura N Borodinsky
Failure of neural tube closure leads to neural tube defects (NTDs), which can have serious neurological consequences or be lethal. Use of antiepileptic drugs (AEDs) during pregnancy increases the incidence of NTDs in offspring by unknown mechanisms. Here we show that during Xenopus laevis neural tube formation, neural plate cells exhibit spontaneous calcium dynamics that are partially mediated by glutamate signaling. We demonstrate that N-methyl-D-aspartate (NMDA) receptors are important for the formation of the neural tube and loss of their function induces an increase in neural plate cell proliferation and impairs neural cell migration, which result in NTDs...
April 30, 2018: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/29712650/activity-of-combinations-of-antistaphylococcal-antibiotics-with-fusidic-acid-against-staphylococcal-biofilms-in-in-vitro-static-and-dynamic-models
#17
Wafi Siala, Hector Rodriguez-Villalobos, Prabhavathi Fernandes, Paul M Tulkens, Françoise Van Bambeke
Staphylococcal biofilms are a major cause of therapeutic failure, especially when caused by multiresistant strains. Oral fusidic acid is currently being re-developed in the US for skin and skin structure and orthopedic infections, in which biofilms play a major role. The aim of this study was to examine the activity of fusidic acid alone or combined with other anti-staphylococcal drugs against biofilms made by a reference strain and five clinical isolates of Staphylococcus aureus or Staphylococcus epidermidis in in vitro static or dynamic models (microtiter plates and CDC reactor) exposed to clinically-relevant concentrations...
April 30, 2018: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/29707436/strain-level-genetic-diversity-of-methylophaga-nitratireducenticrescens-confers-plasticity-to-denitrification-capacity-in-a-methylotrophic-marine-denitrifying-biofilm
#18
Valérie Geoffroy, Geneviève Payette, Florian Mauffrey, Livie Lestin, Philippe Constant, Richard Villemur
Background: The biofilm of a methanol-fed, fluidized denitrification system treating a marine effluent is composed of multi-species microorganisms, among which Hyphomicrobium nitrativorans NL23 and Methylophaga nitratireducenticrescens JAM1 are the principal bacteria involved in the denitrifying activities. Strain NL23 can carry complete nitrate (NO[Formula: see text]) reduction to N2 , whereas strain JAM1 can perform 3 out of the 4 reduction steps. A small proportion of other denitrifiers exists in the biofilm, suggesting the potential plasticity of the biofilm in adapting to environmental changes...
2018: PeerJ
https://www.readbyqxmd.com/read/29704127/correlation-between-femoral-offset-loss-and-dynamic-hip-screw-cut-out-complications-after-pertrochanteric-fractures-a-case-control-study
#19
Baptiste Boukebous, Pascal Guillon, Eric Vandenbussche, Marc Antoine Rousseau
BACKGROUND: Screw-plates disassembly incidence after pertrochanteric fracture (PF) amounts to 1 and 16% among the elderly population. The main occurrence is early cervical screw cut-out. The population at highest risk of disassembly remains difficult to identify. The correlation between femoral offset loss and disassembly occurrence has never been surveyed. OBJECTIVES: A radiological prognosis score for screw plate disassembly was defined to reflect trochanteric impaction (TI); it was based on a femoral offset ratio...
April 27, 2018: European Journal of Orthopaedic Surgery & Traumatology: Orthopédie Traumatologie
https://www.readbyqxmd.com/read/29694206/in-situ-x-ray-diffraction-of-shock-compressed-fused-silica
#20
Sally June Tracy, Stefan J Turneaure, Thomas S Duffy
Because of its widespread applications in materials science and geophysics, SiO_{2} has been extensively examined under shock compression. Both quartz and fused silica transform through a so-called "mixed-phase region" to a dense, low compressibility high-pressure phase. For decades, the nature of this phase has been a subject of debate. Proposed structures include crystalline stishovite, another high-pressure crystalline phase, or a dense amorphous phase. Here we use plate-impact experiments and pulsed synchrotron x-ray diffraction to examine the structure of fused silica shock compressed to 63 GPa...
March 30, 2018: Physical Review Letters
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