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https://www.readbyqxmd.com/read/27913249/comparison-of-the-transcriptomic-profile-of-hepatic-human-induced-pluripotent-stem-like-cells-cultured-in-plates-and-in-a-3d-microscale-dynamic-environment
#1
Eric Leclerc, Keiichi Kimura, Marie Shinohara, Mathieu Danoy, Morgane Le Gall, Taketomo Kido, Atsushi Miyajima, Teruo Fujii, Yasuyuki Sakai
We have compared the transcriptomic profiles of human induced pluripotent stem cells after their differentiation in hepatocytes like cells in plates and microfluidic biochips. The biochips provided a 3D and dynamic support during the cell differentiation when compared to the 2D static cultures in plates. The microarray have demonstrated the up regulation of important pathway related to liver development and maturation during the culture in biochips. Furthermore, the results of the transcriptomic profile, coupled with immunostaining, and RTqPCR analysis have shown typical biomarkers illustrating the presence of responders of biliary like cells, hepatocytes like cells, and endothelial like cells...
November 29, 2016: Genomics
https://www.readbyqxmd.com/read/27911397/nanostructured-ag-zeolite-composites-as-luminescence-based-humidity-sensors
#2
Eduardo Coutino-Gonzalez, Wouter Baekelant, Bjorn Dieu, Maarten B J Roeffaers, Johan Hofkens
Small silver clusters confined inside zeolite matrices have recently emerged as a novel type of highly luminescent materials. Their emission has high external quantum efficiencies (EQE) and spans the whole visible spectrum. It has been recently reported that the UV excited luminescence of partially Li-exchanged sodium Linde type A zeolites [LTA(Na)] containing luminescent silver clusters can be controlled by adjusting the water content of the zeolite. These samples showed a dynamic change in their emission color from blue to green and yellow upon an increase of the hydration level of the zeolite, showing the great potential that these materials can have as luminescence-based humidity sensors at the macro and micro scale...
November 15, 2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/27910495/cross-calibration-of-fuji-tr-image-plate-and-rar-2492-x-ray-film-to-determine-the-response-of-a-ditabis-super-micron-image-plate-scanner
#3
G Dunham, E C Harding, G P Loisel, P W Lake, L B Nielsen-Weber
Fuji TR image plate is frequently used as a replacement detector medium for x-ray imaging and spectroscopy diagnostics at NIF, Omega, and Z facilities. However, the familiar Fuji BAS line of image plate scanners is no longer supported by the industry, and so a replacement scanning system is needed. While the General Electric Typhoon line of scanners could replace the Fuji systems, the shift away from photo stimulated luminescence units to 16-bit grayscale Tag Image File Format (TIFF) leaves a discontinuity when comparing data collected from both systems...
November 2016: Review of Scientific Instruments
https://www.readbyqxmd.com/read/27910405/high-dynamic-range-neutron-time-of-flight-detector-used-to-infer-the-d-t-n-4-he-and-d-d-n-3-he-reaction-yield-and-ion-temperature-on-omega
#4
C J Forrest, V Yu Glebov, V N Goncharov, J P Knauer, P B Radha, S P Regan, M H Romanofsky, T C Sangster, M J Shoup, C Stoeckl
Upgraded microchannel-plate-based photomultiplier tubes (MCP-PMT's) with increased stability to signal-shape linearity have been implemented on the 13.4-m neutron time-of-flight (nTOF) detector at the Omega Laser Facility. This diagnostic uses oxygenated xylene doped with diphenyloxazole C15H11NO + p-bis-(o-methylstyryl)-benzene (PPO + bis-MSB) wavelength shifting dyes and is coupled through four viewing ports to fast-gating MCP-PMT's, each with a different gain to allow one to measure the light output over a dynamic range of 1 × 10(6)...
November 2016: Review of Scientific Instruments
https://www.readbyqxmd.com/read/27908499/a-strategy-to-modulate-the-electrophoretic-behavior-in-plastic-microchips-using-sodium-polystyrene-sulfonate
#5
Jinxiu Guo, Yu Chen, Lizhi Zhao, Ping Sun, Hongli Li, Lei Zhou, Xiayan Wang, Qiaosheng Pu
Plastic microchips have been broadly used as disposable microfluidic devices, but the poorly defined surface properties limit their application. Herein, we proved that an anionic polymer could be used as the background electrolyte (BGE) to provide a strong and stable cathodic electroosmotic flow (EOF) and modulate the electrophoretic behavior for efficient separation in relative thicker microchannels (∼75μm id). A cathodic EOF of ∼3.3×10(-4)cm(2)V(-1)s(-1) was maintained using sodium polystyrene sulfonate (PSSNa) with a molecular weight of 5×10(5) as the BGE, which ensured fluorescein isothiocyanate labeled biogenic amines (BAs) appeared ahead of other components in the electropherograms obtained with microchips of cyclic olefin copolymer...
November 23, 2016: Journal of Chromatography. A
https://www.readbyqxmd.com/read/27906352/tunable-pancharatnam-berry-metasurface-for-dynamical-and-high-efficiency-anomalous-reflection
#6
He-Xiu Xu, Guang-Ming Wang, Tong Cai, Jun Xiao, Ya-Qiang Zhuang
Pancharatnam-Berry (PB) metasurfaces have intrigued a great deal of interest in recent years for anomalous reflection/refraction, vortex plate, orbital angular momentum, flat lens, photonic spin hall effect (PSHE), holograms and reflect/transmit arrays. However, almost all designs are restricted to fixed electrical performance/functionality once the design is finished. Here, we report for the first time a strategy for PB metasurface with agile working frequency by involving each meta-atom with tunable PIN diodes...
November 28, 2016: Optics Express
https://www.readbyqxmd.com/read/27904716/is-anterior-bridge-plating-for-mid-shaft-humeral-fractures-a-suitable-option-for-patients-predominantly-involved-in-overhead-activities-a-functional-outcome-study-in-athletes-and-manual-laborers
#7
Abhishek S Mahajan, Young Gun Kim, Jae Hwa Kim, Prashanth D'sa, Azhar Lakhani, Hyun Soo Ok
BACKGROUND: To assess the functional and radiological outcomes of minimally invasive anterior bridge plating (ABP) for mid-shaft humerus fractures in patients predominantly involved in overhead activities (athletes and manual laborers). METHODS: Forty-eight patients fulfilling inclusion criteria were treated with ABP at a level-I trauma center using a 4.5-mm dynamic/locking compression plate and followed for a period of 1 year. Functional outcome was assessed using the Constant, Mayo elbow, and Disabilities of the Arm, Shoulder and Hand (DASH) scores...
December 2016: Clinics in Orthopedic Surgery
https://www.readbyqxmd.com/read/27903020/dynamic-decompressive-craniotomy-with-a-novel-reversibly-expandable-plate
#8
Rohit Khanna
Objective To assess the feasibility of a dynamic craniotomy procedure with the use of a novel reversibly expandable cranial bone flap fixation plate. The expandable plate allows outward bone flap migration with an increase in intracranial volume or intracranial pressure (ICP). Methods Dynamic craniotomy intracranial hypertension compliance was evaluated in a skull model with progressive increase in intracranial volume and compared with the standard craniotomy with fixed plates. Results Dynamic craniotomy provided significant control of ICP with increasing intracranial volume compared with the standard craniotomy...
November 30, 2016: Journal of Neurological Surgery. Part A, Central European Neurosurgery
https://www.readbyqxmd.com/read/27900192/vertical-stiffness-is-not-related-to-anterior-cruciate-ligament-elongation-in-professional-rugby-union-players
#9
Benjamin G Serpell, Jennie M Scarvell, Mark R Pickering, Nick B Ball, Diana Perriman, John Warmenhoven, Paul N Smith
BACKGROUND: Novel research surrounding anterior cruciate ligament (ACL) injury is necessary because ACL injury rates have remained unchanged for several decades. An area of ACL risk mitigation which has not been well researched relates to vertical stiffness. The relationship between increased vertical stiffness and increased ground reaction force suggests that vertical stiffness may be related to ACL injury risk. However, given that increased dynamic knee joint stability has been shown to be associated with vertical stiffness, it is possible that modification of vertical stiffness could help to protect against injury...
2016: BMJ Open Sport & Exercise Medicine
https://www.readbyqxmd.com/read/27898451/the-effect-of-dynamic-versus-static-plating-systems-on-fusion-rates-and-complications-in-1-level-and-or-2-level-anterior-cervical-discectomy-and-fusion-a-systematic-review
#10
Gregory D Schroeder, Christopher K Kepler, Douglas A Hollern, Ricardo Rodrigues-Pinto, Mark F Kurd, Jefferson R Wilson, Mitchell G Maltenfort, Jonathan T Paul, Andrew N Fleischman, Kathryn Dwight, Paul W Millhouse, Alexander R Vaccaro
STUDY DESIGN: A systematic review. OBJECTIVE: To determine the effect of plate design on fusion rates in patients undergoing a 1- and 2-level anterior cervical discectomy and fusion (ACDF). METHODS: Articles published between January 1, 2002 and January 1, 2015 were systematically reviewed to determine the fusion rate of 1- and 2-level ACDFs using either a fully constrained or semiconstrained locking plate. Additional variables that were collected included the number of levels, the type of graft/cage used, the study design, the method for determining fusion, and complications...
November 28, 2016: Clinical Spine Surgery
https://www.readbyqxmd.com/read/27896682/methods-of-power-force-velocity-profiling-during-sprint-running-a-narrative-review
#11
REVIEW
Matt R Cross, Matt Brughelli, Pierre Samozino, Jean-Benoit Morin
The ability of the human body to generate maximal power is linked to a host of performance outcomes and sporting success. Power-force-velocity relationships characterize limits of the neuromuscular system to produce power, and their measurement has been a common topic in research for the past century. Unfortunately, the narrative of the available literature is complex, with development occurring across a variety of methods and technology. This review focuses on the different equipment and methods used to determine mechanical characteristics of maximal exertion human sprinting...
November 28, 2016: Sports Medicine
https://www.readbyqxmd.com/read/27893564/instrumented-test-of-sensory-integration-for-balance-a-validation-study
#12
Lynn Freeman, Geetanjali Gera, Fay B Horak, Mary T Blackinton, Mark Besch, Laurie King
BACKGROUND AND PURPOSE: Abnormal postural sway is associated with an increase in risk of falls but is difficult for clinicians to accurately quantify without access to laboratory equipment. Instrumenting clinical outcome measures using body-worn movement monitors is a low-cost alternative. This is the first study to compare the modified Clinical Test of Sensory Integration for Balance (i-mCTSIB) to the laboratory test of the Sensory Organization Test (SOT) with dynamic posturography in a group of participants with Parkinson's disease (PD) and subtle balance limitations...
November 23, 2016: Journal of Geriatric Physical Therapy
https://www.readbyqxmd.com/read/27891409/distal-femur-locking-plate-the-answer-to-all-distal-femoral-fractures
#13
Jagandeep Singh Virk, Sudhir Kumar Garg, Parmanand Gupta, Vivek Jangira, Jagdeep Singh, Sudhir Rana
INTRODUCTION: Good results have been published by researchers with distal femur nail, dynamic condylar screw and even addition of a medial plate to a distal femur locking plate for treating distal femur fractures. By this study, we explore the capability of a distal femur locking plate to counter distal femur fractures of extra- articular, partial or intra- articular nature. Positive results have been published by various groups from all over the world. AIM: To study the functional and radiological outcome of distal femoral fractures in skeletally mature patients treated by open reduction and internal fixation with distal femur locking plate...
October 2016: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/27885262/comparison-of-the-effect-on-bone-healing-process-of-different-implants-used-in-minimally-invasive-plate-osteosynthesis-limited-contact-dynamic-compression-plate-versus-locking-compression-plate
#14
Zichao Xue, Haitao Xu, Haoliang Ding, Hui Qin, Zhiquan An
Minimally invasive plate osteosynthesis (MIPO) has been widely accepted because of its satisfactory clinical outcomes. However, the implant construct that works best for MIPO remains controversial. Different plate designs result in different influence mechanisms to blood flow. In this study, we created ulnar fractures in 42 beagle dogs and fixed the fractures using MIPO. The dogs were randomly divided into two groups and were fixed with a limited contact dynamic compression plate (LC-DCP) or a locking compression plate (LCP)...
November 25, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27875995/high-throughput-gene-expression-quantification-of-grapevine-defense-responses-in-the-field-using-microfluidic-dynamic-arrays
#15
Marie-Cécile Dufour, Noël Magnin, Bernard Dumas, Sophie Vergnes, Marie-France Corio-Costet
BACKGROUND: The fight against grapevine diseases due to biotrophic pathogens usually requires the massive use of chemical fungicides with harmful environmental effects. An alternative strategy could be the use of compounds able to stimulate plant immune responses which significantly limit the development of pathogens in laboratory conditions. However, the efficiency of this strategy in natura is still insufficient to be included in pest management programs. To understand and to improve the mode of action of plant defense stimulators in the field, it is essential to develop reliable tools that describe the resistance status of the plant upon treatment...
November 22, 2016: BMC Genomics
https://www.readbyqxmd.com/read/27866606/effect-of-gemini-surfactant-16-6-16-on-the-synthesis-of-silver-nanoparticles-a-facile-approach-for-antibacterial-application
#16
A Mohammed Siddiq, Thanusu Parandhaman, A Farzana Begam, Sujoy K Das, Md Sayem Alam
In this report, we describe the effect of Gemini surfactants1, 6-Bis (N, N-hexadecyldimethylammonium) adipate (16-6-16) on synthesis, stability and antibacterial activity of silver nanoparticles (AgNPs). The stabilizing effect of Gemini surfactant and aggregation behavior of AgNPs was evaluated by plasmonic property and morphology of the AgNPs were characterized by UV-vis spectroscopy, Dynamic Light Scattering (DLS), X-ray diffraction (XRD), High resolution transmission electron microscopy (HRTEM) and Energy dispersive X-ray analysis (EDX) techniques...
December 2016: Enzyme and Microbial Technology
https://www.readbyqxmd.com/read/27861456/dynamic-stabilization-of-simple-fractures-with-active-plates-delivers-stronger-healing-than-conventional-compression-plating
#17
Michael Bottlang, Stanley Tsai, Emily K Bliven, Brigitte von Rechenberg, Philipp Kindt, Peter Augat, Julia Henschel, Daniel C Fitzpatrick, Steven M Madey
OBJECTIVES: Active plates dynamize a fracture by elastic suspension of screw holes within the plate. We hypothesized that dynamic stabilization with active plates delivers stronger healing relative to standard compression plating. METHODS: Twelve sheep were randomized to receive either a standard compression plate (CP) or an active plate (ACTIVE) for stabilization of an anatomically reduced tibial osteotomy. In the CP group, absolute stabilization was pursued by interfragmentary compression with six cortical screws...
November 16, 2016: Journal of Orthopaedic Trauma
https://www.readbyqxmd.com/read/27857950/the-thermal-probe-test-a-novel-behavioral-assay-to-quantify-thermal-paw-withdrawal-thresholds-in-mice
#18
Jennifer R Deuis, Irina Vetter
Rodent models are frequently used to improve our understanding of the molecular mechanisms of pain and to develop novel analgesics. Robust behavioral assays that quantify nociceptive responses to different sensory modalities, such has heat, are therefore needed. Here, we describe a novel behavioral assay to quantify thermal paw withdrawal thresholds in mice, called the thermal probe test, and compared it with other methods commonly used to measure heat thresholds, namely the Hargreaves test and the dynamic and conventional hot plate tests...
April 2016: Temperature: Multidisciplinary Biomedical Journal
https://www.readbyqxmd.com/read/27845350/tunable-graphene-based-mid-infrared-plasmonic-wide-angle-narrowband-perfect-absorber
#19
Hongju Li, Lingling Wang, Xiang Zhai
In this paper, the periodic double-layer graphene ribbon arrays placed near a metallic ground plate coated by a dielectric layer are proposed and analyzed by the coupled-mode theory (CMT) to predict the perfect absorption response in the mid-infrared region. Numerical simulations of the finite-difference time-domain (FDTD) method confirm this effect and give the underlying physical origin. The anti-symmetric dipole-dipole coupling mode is supported by the double-layer graphene ribbons and acts as the electrical resonance to suppress the reflection, because of the impedance matching...
November 15, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27844108/transcriptional-responses-of-the-bacterium-burkholderia-terrae-bs001-to-the-fungal-host-lyophyllum-sp-strain-karsten-under-soil-mimicking-conditions
#20
Irshad Ul Haq, Francisco Dini-Andreote, Jan Dirk van Elsas
In this study, the mycosphere isolate Burkholderia terrae BS001 was confronted with the soil fungus Lyophyllum sp. strain Karsten on soil extract agar plates in order to examine its transcriptional responses over time. At the initial stages of the experiment (T1-day 3; T2-day 5), contact between both partner organisms was absent, whereas in the final stage (T3-day 8), the two populations made intimate physical contact. Overall, a strong modulation of the strain BS001 gene expression patterns was found. First, the stationary-phase sigma factor RpoS, and numerous genes under its control, were strongly expressed as a response to the soil extract agar, and this extended over the whole temporal regime...
November 14, 2016: Microbial Ecology
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