Closed kinetic chain

Hybridization chain reaction (HCR) based enzyme-free amplification techniques have recently been developed for the visualization of intracellular messenger RNA (mRNA). However, the slow kinetics and potential interference with the intricate biological environments hinder its application in the clinic and in vivo. Herein, we designed a nanofirecracker probe-based strategy using intramolecular hybridization chain reaction (IHCR) amplifier for rapid, efficient, sensitive, specific detection and imaging of survivin mRNA both in vitro and vivo...

As the number of musculoskeletal disorders caused by smartphone usage, sedentary lifestyles, and active sports activities increases, there is a growing demand for precise and accurate measurement and evaluation of issues such as incorrect compensation patterns, asymmetrical posture, and limited joint operation range. Urgent development of new inspection equipment is necessary to address issues such as convenience, economic feasibility, and post-processing difficulties. Using 4DEYE® , a new multi-view red, green, and blue (RGB) sensor-based motion analysis equipment, and the VICON® ratio, which are infrared-based markers, we conducted a comparative analysis of the simultaneous validity of the joint angle (trajectory) and reliability...

PURPOSE: This study aimed to comprehensively investigate the global implementation of flywheel training (FT) by basketball strength and conditioning (S&C) coaches in various contexts, encompassing daily practice, games, and sessions. METHODS: Survey data were collected from 117 basketball S&C coaches who participated in a 24-question online survey. The survey was structured into 6 key areas, including country and competition, S&C coach context, training methodology, flywheel and competition, postactivation performance enhancement, and recovery...

Aggregation of transthyretin (TTR) is associated with devastating amyloid diseases. Amyloidosis begins with the dissociation of the native homotetramer (a dimer of dimers) to form a monomeric intermediate that assembles into pathogenic aggregates. This process is accelerated in vitro at low pH, but the process by which TTR dissociates and reassembles at neutral pH remains poorly characterized due to the low population of intermediates. Here, we use 19 F-nuclear magnetic resonance (NMR) and a highly sensitive trifluoromethyl probe to determine the relative populations of the species formed by the dissociation of a destabilized variant, A25T...

OBJECTIVES: To investigate the effects of cross-education on RC muscle strength recovery and shoulder function in patients with arthroscopic anterior shoulder stabilization surgery. METHODS: Twenty-eight patients with shoulder stabilization surgery were included in the study (age: 25±6 years and body-mass index: 24.8±3.6 kg/m2 ). The patients were randomly divided into either cross-education (n=14) or control (n=14) groups. All patients received a standardized rehabilitation program until the end of the postoperative 12th week...

Extensive computer simulation studies have been carried out to probe the pH-dependent structure and dynamics of the two most efficient isoenzymes II and IX of human carbonic anhydrase (HCA) that control the pH in the human body. The equilibrium structure and hydration of their catalytic domains are found to be largely unaffected by the variation of pH in the range studied, in close agreement with the known experimental results. In contrast, a significant effect of the change in pH is observed for the first time on the local electrostatic potential of the active site walls and the dynamics of active site water molecules...

BACKGROUND: Pectoralis Major ruptures normally occur in activities and sports such as powerlifting and American football or rugby. It is a rarely reported phenomena in professional footballers. Surgery is normally considered the choice of management to enable a safe return to play with conservatively managed ruptures often resulting in significant strength deficits. This case report highlights the management of a pectoralis major rupture in a professional footballer. CASE PRESENTATION: A 27-year-old professional footballer sustained a left pectoralis major rupture after falling to the floor...

Although beach handball is a popular sport worldwide, information about the functional profile in elite athletes are lacking. This study generate a descriptive profile of stability, performance and upper and lower extremities ROM in elite beach handball athletes. Secondary proposals were to compare these outcome measures between sexes and sides, and to report the frequency of athletes "at risk" according to cutoff values for shoulder ROM and dynamic postural stability of the lower extremities. Twenty-eight (n = 16 males, 28...

Commercial food and l-amino acid industries rely on bioengineered d-amino acid oxidizing enzymes to detect and remove d-amino acid contaminants. However, the bioengineering of enzymes to generate faster biological catalysts has proven difficult as a result of the failure to target specific kinetic steps that limit enzyme turnover, k cat , and the poor understanding of loop dynamics critical for catalysis. Pseudomonas aeruginosa d-arginine dehydrogenase ( Pa DADH) oxidizes most d-amino acids and is a good candidate for application in the l-amino acid and food industries...

BACKGROUND: In air pistol shooters, the number of studies evaluating the physical characteristics is very few. The purpose of this study was to examine the relationship of core, scapula, and shoulder girdle muscles endurance on performance in air pistol shooters and to determine the importance of the endurance parameters. METHODS: Twenty-eight male air pistol shooters were included. The Closed Kinetic Chain Upper Extremity Stability Test, Upper Quarter Y Balance Test, Prone Bridge Test, Side Bridge Endurance Test, and The Scapular Muscle Endurance Test were used to evaluate the endurance of shoulder girdle, core, and scapular muscles...

OBJECTIVE: The objective of this study is to describe the mechanism of injury and situational patterns (based on ball possession and playing action leading to injury) of severe (lay-off time >28 days) lower limb muscle injuries in professional male football (soccer) players during match play. METHODS: Players experiencing a severe muscle injury of the lower limb during Italian first (Serie A) division male football matches over three consecutive seasons (2018-2021) were identified...

Palmer, TG, and McCabe, M. The effect of a novel weight-supported kinetic chain resistance training program on proximal core muscular endurance, trunk-to-arm muscular power, and bat swing speed. J Strength Cond Res 37(11): 2130-2140, 2023-Muscular stability and muscular power at the proximal core segments of the pelvis, spine, and trunk are essential attributes in maximizing bat swing speed in the sport of softball. Weight-supported kinetic chain resistance training (WsKC) is a novel closed kinetic chain technique that provides synergistic multiplanar stressors to the proximal core segments via the lower and upper extremities while limiting joint compression and sheer forces throughout the kinetic chain...

Electrical conductivity measurements of polyethylene indicate that the semicrystalline structure and morphology influence the conductivity. To include this effect in atomistic charge transport simulations, models that explicitly or implicitly take morphology into account are required. In the literature, charge transport simulations of amorphous polyethylene have been successfully performed using short oligomers to represent the polymer. However, a more realistic representation of the polymer structure is desired, requiring the development of fast and efficient charge transport algorithms that can handle large molecular systems through coarse-graining...

OBJECTIVE: The aim of this study was to evaluate the impact of anthropometric data and the level of physical activity on the CKCUEST score on uninjured subjects. METHOD: Subjects were eligible for the study if they were aged between 18 and 25 years old. Anthropometric data were collected, and the physical activity level was assessed using the Global Physical Activity Questionnaire (GPAQ). The primary endpoint was the CKCUEST score. The CKCUEST was performed three times and the score was the mean of the 3 tries...

BACKGROUND: While there have been reliability studies conducted on several upper extremity functional performance tests (UEFPT), there are several complicating factors that inhibit the ability to draw definitive consensus about the reliability of the tests in both females and males. Having reliability estimates for UEFPT in the same cohort facilitates direct comparison of their relative and absolute reliability. PURPOSE: To establish the test-retest reliability of the closed kinetic chain upper extremity stability test (CKCUEST), seated medicine ball chest pass test (SMBCPT) and hands-release push-up test (HRPUT) in a cohort of males and females with a history of non-overhead sport participation...

BACKGROUND: Although the important roles of proprioception and neuromuscular control in carpal instabilities under laboratory conditions have been recognized, only a few studies have translated this knowledge into a routine clinical practice. PURPOSE: This study aimed to evaluate the results of a personalized rehabilitation in patients with carpal instability on functionality and pain intensity. STUDY DESIGN: This was a case series study. METHODS: This case series included 39 adults (mean age: 38...

The Antarctic is the most isolated region in the world; nevertheless, it has not avoided the negative impact of human activity, including the inflow of toxic mercury (Hg). Hg deposited in the Antarctic marine environment can be bioavailable and accumulate in the food web, reaching elevated concentrations in high-trophic-level biota, especially if methylated. Zooplankton, together with phytoplankton, are critical for the transport of pollutants, including Hg to higher trophic levels. For the Southern Ocean ecosystem, one of the key zooplankton components is the Antarctic krill Euphausia superba, the smaller euphausiid Thysanoessa macrura, and the amphipod Themisto gaudichaudii - a crucial food source for most predatory fish, birds, and mammals...

The development of nonprecious metal catalysts to meet the activity-stability balance at industrial-grade large current densities remains a challenge toward practical alkali-water electrolysis. Here, we develop an orderly nanodendritic nickel (ND-Ni) catalyst that consists of ultrafine nanograins in chain-like conformation, which shows both excellent activity and robust stability for large-current-density hydrogen evolution reaction (HER) in alkaline media, superior to currently-applied Raney nickel (R-Ni) catalyst in commercial alkali-water electrolyser (AWE)...

Quaternary ammonium compounds (QACs) have been extensively used in the community, healthcare facilities, and food chain, in concentrations between 20 and 30,000 mg/L. Enterococcus faecalis and Enterococcus faecium are ubiquitous in these settings and are recognized as nosocomial pathogens worldwide, but QACs' activity against strains from diverse epidemiological and genomic backgrounds remained largely unexplored. We evaluated the role of Enterococcus isolates from different sources, years, and clonal lineages as hosts of QACs tolerance genes and their susceptibility to QACs in optimal, single-stress and cross-stress growth conditions...

Soluble aggregates are reported to be the most neurotoxic species of α-Synuclein (αSyn) in Parkinson's disease (PD) and hence are a promising target for diagnosis and treatment of PD. However, the predominantly intracellular location of αSyn limits its accessibility, especially for antibody-based molecules and prompts the need for exceptionally strong soluble αSyn aggregate binders to enhance their sensitivity and efficacy for targeting the extracellular αSyn pool. In this study, we have created the multivalent antibodies TetraSynO2 and HexaSynO2, derived from the αSyn oligomer-specific antibody SynO2, to increase avidity binding to soluble αSyn aggregate species through more binding sites in close proximity...