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Jia You, Ying Shi, Wenli Zhu, Zhigang Wu, Jingyuan Xiong
There are two types of disulfide linkages in IgG antibodies at the hinge region: intra- and inter-disulfide linkage. Characterization of intra-disulfide linked isomer will provide important information on the stability of the antibodies and better understanding of the mechanism of Fab-arm exchange. In this report, HPLC coupled with high resolution mass spectrometry was applied for characterization of disulfide linkages in IgG antibodies at the hinge region. We were able to accurately identify both inter- and intra-disulfide linked peptides and correctly quantify intra-disulfide isomers...
August 19, 2018: Biomedical Chromatography: BMC
D V Grishin, D D Zhdanov, Yu A Gladilina, O V Podobed, V S Pokrovsky, M V Pokrovskaya, S S Aleksandrova, N N Sokolov
Mutant homologues of small chemotactic and DNA-binding proteins from thermophilic bacteria Thermotoga petrophila RKU-1 and Thermotoga naphthophila were obtained. These proteins can be expressed in the recombinant form in E. coli cells. A wide range of properties and parameters that are important for isolation of these proteins were revealed: stability in a wide temperature and pH range, high level of expression, solubility, and the possibility of using simple purification schemes with low number of successive steps...
August 18, 2018: Bulletin of Experimental Biology and Medicine
Avril Mansfield, Anthony Aqui, Cynthia J Danells, Svetlana Knorr, Andrew Centen, Vincent G DePaul, Alison Schinkel-Ivy, Dina Brooks, Elizabeth L Inness, George Mochizuki
OBJECTIVES: No intervention has been shown to prevent falls poststroke. We aimed to determine if perturbation-based balance training (PBT) can reduce falls in daily life among individuals with chronic stroke. DESIGN: Assessor-blinded randomised controlled trial. SETTING: Two academic hospitals in an urban area. INTERVENTIONS: Participants were allocated using stratified blocked randomisation to either 'traditional' balance training (control) or PBT...
August 17, 2018: BMJ Open
Qinghua Wang, Jing Tang, Shujun Jiang, Zan Huang, Anying Song, Siyuan Hou, Xiang Gao, Hai-Bin Ruan
Peroxisome proliferator-activated receptor-γ (PPARγ) is a master regulator of adipogenesis and a target of the thiazolidinedione (TZD) class of antidiabetic drugs; therefore, identifying novel regulators of PPARγ action in adipocytes is essential for the future development of therapeutics for diabetes. MAGE family member D1 (MAGED1), by acting as an adaptor for ubiquitin-dependent degradation pathways and a co-factor for transcription, plays an important role in neural development, cell differentiation, and circadian rhythm...
August 18, 2018: Journal of Endocrinology
Yongcui Wang, Lei Chu, Stefani Daryanto, Linyou Lü, MuSa Ala, Lixin Wang
Currently the amount of data available on the effect of sand dune stabilization on species conservation in inter-dune lowland is very limited, especially for the sand dune systems in semi-arid regions. In this study, we determined whether the characteristics of above-ground vegetation, soil seed bank and their relationships with environmental factors changed with sand dune stabilization in the inter-dune lowlands in Horqin Sandy Land, China. Species composition, abundance and coverage of aboveground vegetation as well as soil seed bank composition and density were surveyed and their correlations with environmental factors (pH, organic matter content, total nitrogen and total phosphorus) were determined...
August 7, 2018: Science of the Total Environment
Haifeng Ji, Xin Song, Chao He, Chengqiang Tang, Lian Xiong, Weifeng Zhao, Changsheng Zhao
Ion exchange materials show great advantages in water purification, food industry, pharmaceutical industry, etc. However, the ion exchange capacities of ion exchange materials, especially for anion-exchange materials, at present are still relatively low. Hydrogels own abundant functional groups and show high hydrophilicity, and thus are recognized as high-potential ion exchange materials, but may deform and even crush in use due to their low mechanical strength and unavoidable swelling behavior. In this study, inspired by the root-soil structure, novel poly(methacryloxyethyltrimethyl ammonium chloride) composite hydrogel microspheres with ultrahigh ion exchange capacity (more than 3...
August 11, 2018: Journal of Colloid and Interface Science
Zhi Wang, Weizhou Xu, Jiyue Kang, Min Li, Jin Huang, Qingbo Ke, Ho Soo Kim, Bingcheng Xu, Sang-Soo Kwak
The multifunctional Orange (Or) protein plays crucial roles in carotenoid homeostasis, photosynthesis stabilization, and antioxidant activity in plants under various abiotic stress conditions. The Or gene has been cloned in several crops but not in alfalfa (Medicago sativa L.). Alfalfa is widely cultivated across the world; however, its cultivation is largely limited by various abiotic stresses, including drought. In this study, we isolated the Or gene from alfalfa (MsOr) cv. Xinjiang Daye. The amino acid sequence of the deduced MsOr protein revealed that the protein contained two trans-membrane domains and a DnaJ cysteine-rich zinc finger domain, and showed a high level of similarity with the Or protein of other plants species...
August 14, 2018: Plant Physiology and Biochemistry: PPB
Rui Guo, Wenya Zhang, Ying Yang, Jian Ding, Wenzhi Yang, Yingmei Zhang
Environmental pollution is known to adversely affect amphibian reproduction and survival, however, the knowledge of environmental heavy metal pollution on fitness of male amphibian is largely unknown. The present study aimed to explore the variation in fitness of male Bufo raddei, a widespread anuran in northwest China, subjected to long-term heavy metal stress in Baiyin (BY) city. BY is heavily polluted by heavy metals mainly copper, zinc, lead and cadmium; meanwhile, Liujiaxia (LJX), a relatively unpolluted area, was chosen as control...
August 16, 2018: Ecotoxicology and Environmental Safety
T Yadav, V Mukherjee
This work deals with the molecular modeling and vibrational spectra of all the twenty conformers of an important biomolecule octopamine which have been investigated using the DFT/B3LYP level of theory in combination with the 6-31++g(d,p) as a suitable basis set. The experimental FTIR and FTRaman spectra of octopamine neurotransmitter were recorded in the spectral region 400-4000 cm-1 and 50-4000 cm-1 respectively and correlated with the calculated spectra of the most stable conformer. The effect of hydrochloride on the important geometrical parameters of most stable conformer of octopamine was also studied...
August 10, 2018: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
Lukasz J Ochyl, Joseph D Bazzill, Charles Park, Yao Xu, Rui Kuai, James J Moon
Despite the promise and advantages of autologous cancer cell vaccination, it remains challenging to induce potent anti-tumor immune responses with traditional immunization strategies with whole tumor cell lysate. In this study, we sought to develop a simple and effective approach for therapeutic vaccination with autologous whole tumor cell lysate. Endogenous cell membranes harvested from cancer cells were formed into PEGylated nano-vesicles (PEG-NPs). PEG-NPs exhibited good serum stability in vitro and draining efficiency to local lymph nodes upon subcutaneous administration in vivo...
August 9, 2018: Biomaterials
Hugo Ten Cate, Anthonie W A Lensing, Jeffrey I Weitz, Saskia Middeldorp, Jan Beyer-Westendorf, Dagmar Kubitza, Timothey Brighton, Gary E Raskob, Patrick Mismetti, Paolo Prandoni, Martin Gebel, Martin H Prins
INTRODUCTION: For treatment of deep vein thrombosis (DVT) and pulmonary embolism (PE), rivaroxaban is given in fixed doses without routine coagulation monitoring. PATIENTS AND METHODS: To determine whether monitoring would enhance its benefit-risk profile, we examined whether peak and trough prothrombin time (PT) values measured in 3797 rivaroxaban-treated patients included in the EINSTEIN DVT and PE studies correlated with subsequent recurrent VTE and major bleeding...
August 15, 2018: Thrombosis Research
Sandip Kumar Nandi, Ayon Chakraborty, Alok Kumar Panda, Rajiv Kumar Kar, Anirban Bhunia, Ashis Biswas
Mycobacterium leprae uptakes various bivalent metal ions via different transporters in host species. Uptake of Cu2+ and Zn2+ are essential for generation of superoxide dismutases and catalases, which provide defense against reactive oxygen species mediated death of this pathogen in macrophages. Furthermore, it has also been noticed that levels of different bivalent metal ions (Ca2+ , Mg2+ , Cu2+ and Zn2+ ) in blood serum are altered in leprotic patients. Mycobacterium leprae HSP18 is an immunodominant antigen which helps in growth and survival of Mycobacterium leprae in host species...
August 10, 2018: Journal of Inorganic Biochemistry
Martina Quaretti, Marina Porchia, Francesco Tisato, Angela Trapananti, Giuliana Aquilanti, Marko Damjanović, Luciano Marchiò, Marco Giorgetti, Matteo Tegoni
The chemistry of copper(I) with water-soluble phosphines is an emergent area of study which has the objective of finding ligands that stabilize copper in its lower oxidation state. Cu(I) has been found relevant in the mechanism of copper transports into cells, and the accessibility of this oxidation state has implications in oxidative stress processes. For these reasons the possibility to deal with stable, water soluble copper(I) is an attractive approach for devising new biologically relevant metal-based drugs and chelating agents...
August 10, 2018: Journal of Inorganic Biochemistry
Maria Chountoulesi, Natassa Pippa, Stergios Pispas, Evangelia D Chrysina, Aleksander Forys, Barbara Trzebicka, Costas Demetzos
The self-assembly process of amphiphilic molecules into solvents results in different mesophases, such as inverse cubic and hexagonal that both belong to the wider category of lyotropic liquid crystals. The above mesophases can be further exploited upon the formation of liquid crystalline nanoparticles, cubosomes and hexosomes respectively, which may be utilized as drug delivery nanosystems, exhibiting major advantages. In the present study, liquid crystalline nanoparticles were prepared and evaluated in terms of morphology and physicochemical behavior...
August 16, 2018: International Journal of Pharmaceutics
Tyler A Garretson, Hui Shang, Annie K Schulz, Brian V Donohue, Xiao-Wen Cheng
The baculovirus-based bacmid expression vector system has been widely used for protein production in basic research and biotechnological laboratories. Since the first construction of the Autographa californica multiple nucleopolyhedrovirus bacmid (AcBacmid), three more bacmids have been created from Bombyx mori nucleopolyhedrovirus (BmBacmid), Spodoptera exigua nucleopolyhedrovirus (SeBacmid) and Helicoverpa armigera nucleopolyhedrovirus (HaBacmid). Each of these bacmid-derived viruses replicates efficiently in a range of specific and permissive cell types...
August 16, 2018: Virus Research
Hristo Svilenov, Lorenzo Gentiluomo, Wolfgang Friess, Dierk Roessner, Gerhard Winter
The early-stage assessment of the physical stability of new monoclonal antibodies in different formulations is often based on high-throughput techniques that suffer from various drawbacks. Accordingly, new approaches that facilitate the protein formulation development can be of high value to the industry. In this study, a dynamic light scattering (DLS) plate reader is used to measure the aggregation (by means of the increase in the hydrodynamic radius (Rh )) of monoclonal antibody samples that were subject to incubation and subsequent dilution from different concentrations of a denaturing agent, i...
August 16, 2018: Journal of Pharmaceutical Sciences
Youssra K Al-Hilaly, Saskia J Pollack, Janet E Rickard, Michael Simpson, Ana-C Raulin, Thomas Baddeley, Pascale Schellenberger, John M D Storey, Charles R Harrington, Claude M Wischik, Louise C Serpell
Alzheimer's disease (AD) is a tauopathy characterised by pathological fibrillisation of tau protein to form the paired helical filaments (PHFs) which constitute neurofibrillary tangles. The methylthioninium (MT) moiety reverses the proteolytic stability of the PHF core and is in clinical development for treatment of AD in a stable reduced form as leuco-MT (LMT). It has been hypothesised that MT acts via oxidation of cysteine residues which is incompatible with activity in the predominantly reducing environment of living cells...
August 16, 2018: Journal of Molecular Biology
Jianshu Dong, Fahui Li, Feng Gao, Jia Wei, Yajing Lin, Yong Zhang, Jizhong Lou, Guangfeng Liu, Yuhui Dong, Lin Liu, Hongmin Liu, Jiangyun Wang, Weimin Gong
The accurate modification of the tRNAIle anticodon wobble cytosine 34 is critical for AUA decoding in protein synthesis. Archaeal tRNAIle2 cytosine 34 is modified with agmatine in the presence of ATP by TiaS (tRNAIle2 agmatidine synthetase). Yet, no structure of apo-form full length TiaS is available currently. Here, the crystal structures of apo TiaS and a complex of TiaS-agmatine-AMPPCP-Mg are presented, with properly folded zinc ribbon and Cys4-zinc coordination identified. Compared with tRNAIle2 bound form, the architecture of apo TiaS shows a totally different conformation of zinc ribbon...
August 16, 2018: Journal of Molecular Biology
S Kundhavai Natchiar, Sangita Venkataraman, Tina-Marie Mullen, Glen R Nemerow, Vijay S Reddy
We report the revised crystal structure of a pseudo-typed human adenovirus at 3.8 Å resolution that is consistent with the atomic models of minor proteins determined by cryo-electron microscopy (cryo-EM). The diffraction data from multiple crystals were rescaled and merged to increase the data completeness. The densities for the minor proteins were initially identified in the phase-refined omit maps that were further improved by the phases from docked poly-alanine models to build atomic structures. While the trimeric fiber molecules are disordered due to flexibility and imposition of 5-fold symmetry, the remaining major capsid proteins, hexon and penton base (PB) are clearly ordered, with the exception of hypervariable region 1 of hexons, the RGD containing loop and the N-termini of the PB...
August 16, 2018: Journal of Molecular Biology
Katherine R Thorson, Tessa V West
Recent work has demonstrated that people can be influenced by the physiological states of their interaction partners, showing physiological linkage to them from one moment to the next. In a study of unacquainted dyads who interacted for 30 minutes (ndyads  = 47), we examine the novel question: Are people who show physiological linkage to their partners in sympathetic nervous system responding also less stable in their own responses? Understanding this relationship has important implications for how social relationships impact affective functioning and health...
August 16, 2018: Biological Psychology
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